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Закономірності деградації хроматину тимоцитів за променевого ураження щурів: Автореф. дис… канд. біол. наук / Ю.П. Пархомець, Київ. ун-т ім. Т.Шевче

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Ee?anueeee oi?aa?neoao ?iai? Oa?ana Oaa/aiea

Ia?oiiaoeue TH??e Iao?iae/

OAeE 576.315.42

Caeiiii??iino? aeaa?aaeaoe?? o?iiaoeio oeiioeeo?a ca i?iiaiaaiai
o?aaeaiiy uo??a

03.00.01-?aae?ia?ieia?y

Aaoi?aoa?ao aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa
a?ieia?/ieo iaoe

Ee?a-1999

Aeena?oaoe??th ? ?oeiien.

?iaioa aeeiiaia a eaai?aoi??? o?ceei-o?i?/ii? a?ieia?? eaoaae?e a?io?i??
a?ieia?/iiai oaeoeueoaoo Ee?anueeiai oi?aa?neoaoo ?iai? Oa?ana Oaa/aiea.

Iaoeiaee ea??aiee- aeieoi? a?ieia?/ieo iaoe Oeoaecaae/ Ai?en
Ieaenaiae?iae/, i?ioani? eaoaae?e a?io?i?? Ee?anueeiai oi?aa?neoaoo
?iai? Oa?ana Oaa/aiea

Io?oe?ei? iiiiaioe- aeieoi? a?ieia?/ieo iaoe Ae?oaeeia Ieeiea
Ieaenaiae?iae/, i?ia?aeiee iaoeiaee ni?a?ia?oiee a?aeae?eo a?io?ceee
?inoeoooo aenia?eiaioaeueii? iaoieia??, iieieia?? ? ?aae?ia?ieia?? ?i.
?.?. Eaaaoeueeiai IAI Oe?a?ie

aeieoi? a?ieia?/ieo iaoe Aeieo?aiei Ieeiea Iao?iae/, caa?aeoth/ee
eaai?aoi???th a?io?i?? ?inoeoooo aeia?a??ie ? oieneeieia??

?i. E.?. Iaaeaaaey IIC Oe?a?ie

I?ia?aeia onoaiiaa- Oe?a?inueeee iaoeiaee oeaio? ?aae?aoe?eii?
iaaeeoeeie AII Oe?a?ie, a?aeae?e ?aae?aoe?eii? a?io?i??, i. Ee?a

Caoeno aeena?oaoe?? a?aeaoaeaoueny “22“ /a?aiy 1999?. i 12 aiae ia
can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae 26. 001. 24 Ee?anueeiai
oi?aa?neoaoo ?i. Oa?ana Oaa/aiea ca aae?anith: 252127, i. Ee?a, i?iniaeo
Aeooeiaa 2, ei?ion 12 ( a?ioae ), aoae. 215

Iiooiaa aae?ana: 252033. Ee?a-33, aoe. Aieiaeeie?nueea, 64, niaoe?aaea
Ae 26.001.24, a?ieia?/iee oaeoeueoao

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? oi?aa-?neoaoo:
Aieiaeeie?nueea, 58.

Aaoi?aoa?ao ?ic?neaiee “18” o?aaiy 1999 ?.

A/aiee nae?aoa? niaoe?ae?ciaaii?

a/aii? ?aaee, i?ioani? I.A.
A?aeii

1

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue i?iaeaie. Ae?y /eneaiieo aeno?aiaeueieo oaeoi??a,
aeeth/ath/e ?ii?coth/o ?aae?aoe?th ia i?aai?ci, aeeeeea? ?yae
?ioiiaeao?oeeoieo oa aooi?ioiieo noai?a. Iniiaieie i?iyaaie
?ioiiaeao?oeeoo ? ci?ia e?euee?niiai ? noaiiioeyoe?eiiai neeaaeo
?ioiieiiiaoaioieo ee?oei, cieaeaiiy i?ie?oa?aoeaii? aeoeaiino?
e?ioioeeo?a, ?o i?a?aoe?eieo aeanoeainoae. Oeaio?aeueia i?noea o
ooieoe?iioaaii? ?ioiii? nenoaie iaeaaeeoue e?ioioeeoai, yeei oa?aeoa?ia
aeniea ?aae?i/ooeea?noue ( ss?eeei, 1988 ; Aeieo?aiei, 1997 ).

Aeine?aeaeaiiy aieeao ?ii?coth/i? ?aae?aoe?? ia ?ioiio nenoaio, aea/aiiy
oeiiaeo ci?i ? iniaeeainoae no?oeoo?ieo, iaoaaie?/ieo oa
ooieoe?iiaeueieo ii?ooaiue a e?ioioeeoao i?iaeiaaeo? aooe iaei??th ?c
iniiaieo i?iaeai no/anii? ?aae?ia?ieia??. Ca?aeii no/anieo oyaeaiue,
caaeaaeue e?ioi?aeieo ee?oei i?e i?iiaiaaiio o?aaeaii? iia’ycaia c
aaciina?aaei?i iioeiaeaeaiiyi iaia?ai ? no?oeoo?ii? oe?e?niino?
o?iiaoeio ( Aieyineee, 1994; Ao?eaeiaa, 1992 ). A?aeiii, ui o?iiaoei a
e?ioi?aeieo ? aaiiiiaoe/ieo oeaieiao ii?ii?iaieo oaa?ei ?icuaieth?oueny
ia e?aoi? ioeeainiiai o?aaiaioe, ui ooai?ththoue ia aeaeo?ioi?aa?aiao
oa?aeoa?io ea?oeio “ae?aaeie” ( Brown, 1993 ). Iiaeaeueoa aea/aiiy
iaoai?ci?a i?aeio-eeainiiii? aeaa?aaeaoe?? o?iiaoeio iieacaee, ui oeae
i?ioean ?i?oe?th?oueny aiaeiaaiieie ioeeaacaie ? ia? i?noea o aaaaoueio
aeiaaeeao i?ia?aiiaaii? ee?oeiii? caaeaae? ( ss?eeei, 1996; Oeoaecaae/
1997 ).

Aiae?c i?iaeaie caaeaae? e?ioioeeo?a iieaco?, ui o i?ciaii? iieaeoey?ieo
iniia i?ioeano aeaa?aaeaoe?? AeIE, iaoai?ci?a eiai ?aaoeyoe??
aeinyaiooee iaaiee i?ia?an ( Oiaineee, 1996; Oainii, 1997 ).
Aeeneon?eieie caeeoathoueny ieoaiiy ?i?oe?aoe?? oa ?aaoeyoe?? ioneiaeo
iaoai?ci?a o?aaiaioaoe?? o?iiaoeio e?ioioeeo?a i?ney ae?? ia oaa?ei
iecueeeo aeic ?ii?coth/i? ?aae?aoe??, iaia? ae/a?iii? ea?oeie noaio
eiiiainaoi?ieo, caoenii-a?aeiiaieo ? iaoieia?/ieo ?aaeoe?e o
a?aeie?ath/eo ee?oeiao.

Ia aeeeeea? noii?ao, ui eiiieaeni? aeine?aeaeaiiy iieaeoey?ieo
iaoai?ci?a ?icaeoeo caaeaae? ee?oei i?eaaaeooue aei iiaeeaeaiiai
?icoi?iiy i?e?iaee aaaaoueio aaaeeeaeo caoai?thaaiue ethaeeie, ui
iia’ycai? c? ci?iaie iieaciee?a ?ioiii? nenoaie ? a?aee?ethoue iia?
ia?niaeoeae aoaeoeaiiai aieeao ia ?ioiiaeao?oeeoi? noaie a oiiaao ae??
?ii?coth/i? ?aae?aoe??.

Ioaea, i?iaeaia, ye?e i?enay/aia ?iaioa, ? aeooaeueiith ? yaey? niaith
cia/iee oai?aoe/iee ? i?aeoe/iee ?ioa?an.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. Aeena?oaoe?eia
?iaioa ? /anoeiith oaiaoe/iiai ieaio iaoeiai-aeine?aeieo ?ia?o eaoaae?e
a?io?i??, ui aeeiio?oueny ia caiiaeaiiy Eaa?iaoo I?i?no??a Oe?a?ie a?ae
1995 aei 2000 ?., ?

2

aea?ae?a?no?aoe?? 0197 U 003173 “A?io?i?/i? iaoai?cie ?icaeoeo
i?iiaiaaiai o?aaeaiiy i?ney ae?? ?ii?coth/i? ?aae?aoe??”.

Iaoa ? caaea/? aeine?aeaeaiiy. Iaoith aeaii? ?iaioe ? aeine?aeaeaiiy
iaoai?cio ?icaeoeo ? ?aaoeyoe?? aeaa?aaeaoe?? no?oeoo?e o?iiaoeio,
aeeiai?ee ianiaoeeo?/ieo no?ani?ieo ?aaeoe?e a oeiioeeoao oaa?ei, ui
caciaee ii?ii?iaiiy, oa aea/aiiy iniaeeainoae aieeao ia oe? i?ioeane
?aae?iiiaeoeyoi?a-i?eioeiai?aeo.

O a?aeiia?aeiino? c iaoith aoee iinoaaeai? oae? caaaeaiiy: 1.
Aeine?aeeoe aieea ??cieo aeic ?ii?coth/i? ?aae?aoe?? ia aeaa?aaeaoe?th
o?iiaoeio oeiioeeo?a uo??a. Aeyaeoe oiiae i?iyao oeeoiaaiaoe/iiai
aoaeoo, oiaoi, aeico ?ii?coth/i? ?aae?aoe?? oa iaeiaioee /an
noaa?eueiiai oi?ioaaiiy aeene?aoieo cii o?iiaoeio ca oeiii “ae?aaeie”.
2. Aeine?aeeoe iaoai?ci aeaa?aaeaoe?? o?iiaoeio oa noai ?aia?aoe?eii?
nenoaie AeIE oeiioeeo?a uo??a i?e iaeii?aciaiio ?aioaai?anueeiio
ii?ii?iaii? oa ae?? o?ii?/iiai aei?ii?ithaaiiy iecueei? ?ioaineaiino?.
3. Aeine?aeeoe i?ioaie?oe/io aeoeai?noue oeiioeeo?a i?e aieea?
?ii?coth/i? ?aae?aoe??. 4. Aeine?aeeoe aieea oioaeueiiai iaeii?aciaiai
?aioaai?anueeiai aei?ii?ithaaiiy ia noai i?ioean?a ia?aeeniiai ieeneaiiy
e?i?ae?a ( IIE ) a oeiioeeoao. 5. Aea/eoe iiaeeo?eoth/o ae?th
i?eioeiai?aeo ia aeaa?aaeaoe?th AeIE oeiioeeo?a ii?ii?iaieo uo??a.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aia?oa iieacaii, ui
aeaa?aaeaoe?y o?iiaoeio a oeiioeeoao uo??a c oi?ioaaiiyi aeene?aoieo
AeIE-o?aaiaio?a ca oeiii “ae?aaeie” ?icii/eia?oueny /a?ac 3 aiaeeie
i?ney iaeii?aciaiai ?aioaai?anueeiai ii?ii?iaiiy a aeic? 1 A?. O?eaaea
o?ii?/ia ciai?oi? oa aioo??oi? ii?ii?iaiiy c caaaeueiei ?aae?aoe?eiei
iaaaioaaeaiiyi 25 nA? aeeeeea? iiae?aio ea?oeio aeaa?aaeaoe?? o?iiaoeio.
Aeyaeai? caeiiii??iino? ooai?aiiy iaeiiieoeiaeo oa aeaiieoeiaeo ?ic?ea?a
AeIE, ye? a?aeaoaathoueny a o?iiaoei? oeiioeeo?a i?ney ae?? ia uo??a
?ii?coth/i? ?aae?aoe?? a aeic? 1 A?, ?o ca”ycie c ?iaioith ?aia?aoe?eii?
nenoaie ee?oeie.

Aea/ai? oa?aeoa?i? caeiiii??iino? i?io?eaiiy ?aaeoe?e IIE ? anoaiiaeaii,
ui a ii?ii?iaieo oeiioeeoao ?aaeoe?? IIE iathoue oaciao caeaaei?noue, ui
a?aeiia?aea? iniiaiei oa?i?iai aeaa?aaeaoe?? o?iiaoeio.

Aeine?aeaeaii i?ioaie?oe/io aeoeai?noue oeiioeeo?a uo??a, ui caciaee
aieeao ?ii?coth/i? ?aae?aoe?? oa iiaeeo?eoth/i? ae?? i?eioeiai?aeo.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a. Io?eiai? ?acoeueoaoe uiaei
caeiiii??iinoae iaoai?cio aeaa?aaeaoe?? o?iiaoeio oeiioeeo?a ii?ii?iaieo
oaa?ei iathoue, ia?o ca ana, ooiaeaiaioaeueia cia/aiiy, ine?eueee
aeicaieythoue nooo?ai iaa?oiooaaoe ?icaeoie i?ney?aae?aoe?eieo ii?ooaiue
a e?ioi?aeieo ee?oeiao ia iieaeoey?iiio ??ai? ? eaaeaoue a iniia?
?ic?iaee cania?a oe?eani?yiiaaii? ?o ei?aeoe??. Aeine?aeaeaii oa?aeoa?
ianiaoeeo?/ieo ?aaeoe?e oa ci?ie i?ioa?iacieo

3

aeoeaiinoae oa?iaio?a, ui aeiiiaiththoue a?aeii? ia nueiaiaei?oi?e
aeaiue i?eioeeie ? aoaie caaeaae? e?ioi?aeieo ee?oei i?ney ae??
?ii?coth/i? ?aae?aoe??.

?acoeueoaoe aeine?aeaeaiue iiaeooue aooe iieeaaeai? a iniiao i?e
?ic?iaoe? iaoeiai-iaa?oioiaaieo oeyo?a noai?aiiy aoaeoeaieo cania?a,
caeaoieo iiaeeo?eoaaoe ae?th ?ii?coth/i? ?aae?aoe?? ia i?aai?ci ?
nenoaie i?aai?a. Oae, i?eioeiai?ae noeioeth? ?iciaae AeIE, ? oei naiei,
i?enei?th? aiiioic a e?ioi?aeieo ee?oeiao.

Iniaenoee aianie caeiaoaa/a. A i?ioean? aeeiiaiiy aeena?oaoe?? aaoi?
iniaenoi a?ae?a?aa oa e?eoe/ii i?iaiae?coaaa aanue ianya a?o/eciyii? oa
ca?oa?aeii? e?oa?aoo?e noiniaii oaie i?iaaaeaiiai iaoeiaiai
aeine?aeaeaiiy, ? cai?iiiioaaa eiai aieiaio iaoo. Aeena?oaioii
a?ae?a?ai? oa iiaeeo?eiaai? iaoiaee ae??oaiiy iinoaaeaieo caaaeaiue,
?ioa?i?aoiaaii io?eiai? ?acoeueoaoe. Aenia?eiaioaeuei? aeine?aeaeaiiy
i?iaiaeeeenue naiino?eii, a oaeiae ni?eueii c ni?a?ia?oieeaie
eaai?aoi??? o?ceei-o?i?/ii? a?ieia??. A aeena?oaoe?? aeei?enoai?
?acoeueoaoe iaoeiaeo ioae?eaoe?e, a oiio ae /ene? iaienai? o
ni?aaaoi?noa?, aeiey iniaenoi? o/ano? o i?aeaioiaoe? eieaeoeaieo
ioae?eaoe?e -90 %.

Ai?iaaoe?y ?iaioe. Iniiai? iaoa??aee aeena?oaoe?eii? ?iaioe
aeiiia?aeaeeny oa iaaiai?thaaeeny ia 3-io neiiic?oi?, i?enay/aiiio
iai’yo? aeieoi?a a?ieia?/ieo iaoe A.A. Ieoaeeianueei? “Ae?aaiinoeea oa
i?io?eaeoeea iaaaoeaieo iane?aee?a ?aae?aoe??” ( Ee?a, 1997 ), VII
Oe?a?inueeiio a?io?i?/iiio c’?cae? ( Ee?a, 1997 ), ??? c’?cae? ii
?aae?aoe?eiei aeine?aeaeaiiyi ( Iineaa, 1997 ), ??? c”?cae?
Oe?a?inueeiai a?io?ce/iiai oiaa?enoaa ( Oa?e?a, 1998 ),
i?ioani?nueei-aeeeaaeaoeuee?e eiioa?aioe?? EO, 1998.

Ioae?eaoe??. Iniiaiee ci?no aeena?oaoe?eii? ?iaioe aeeeaaeaii o 7
ioae?eaoe?yo ( 3 noaoo?, 4 oace aeiiia?aeae ).

Ia’?i ? no?oeoo?a aeena?oaoe??. Aeena?oaoe?eia ?iaioa neeaaea?oueny c
anooio, iaeyaeo e?oa?aoo?e ( 3 ?icae?ee ), iaoiae?a, ?acoeueoao?a
aeine?aeaeaiue oa ?o iaaiai?aiiy ( 2 ?icae?ee ), caeeth/aiiy, aeniiae?a,
nieneo aeei?enoaii? e?oa?aoo?e ( 160 aeaea?ae ). Aeena?oaoe?y aeeeaaeaia
ia 124 noi??ieao iaoeiiieno.

IAOA??AEE OA IAOIAeE AeINE?AeAEAIUe

Aeine?aeaeaiiy i?iaiaeeeeny ia a?eeo iae?i?eieo uo?ao-naioeyo ianith
130-170 a. Canoiniaoaaee oae? aenia?eiaioaeuei? iiaeae? ii?ii?iaiiy: 1.
Iaeii?aciaa oioaeueia ii?ii?iaiiy oaa?ei a ??cieo aeicao ( 1,29 o 10-2
Ee o ea-1, 2,58 o 10-2 Ee o ea-1, 5,16 o 10-2 Ee o ea-1, 10,32 o 10-2 Ee
o ea-1, 15,48 o 10-2 Ee o ea-1, 20,64 o 10-2 Ee o ea-1 ) i?iaiaeeee ia
?aioaai?anuee?e onoaiiaoe? ?OI-17 ca oiia: o?eueo?e 0,5 ii Nu oa

1 ii Al; iai?oaa 200 eA, neea no?oio 5 iA; oe??ii-oieonia a?aenoaiue 50
ni;

4

iiooaei?noue aeice a iia?o??-8,3 o 10-4 A o ea-1. 2. O?ii?/ia
eiia?iiaaia ciai?oi? oa aioo??oi? ii?ii?iaiiy uo??a i?iaiaeeee i?e
iino?eiiio, ia i?ioyc? 6 i?nyoe?a, ia?aaoaaii? uo??a ia
aenia?eiaioaeuei?e aac? i. *i?iiaeeue. Aioo??oi? ?aae?aoe?eia
iaaaioaaeaiiy noai?thaaeiny ca aeiiiiiaith ?aoe?iio i?noeaaiai
ae?iaieoeoaa. Caaaeueia ?aae?aoe?eia iaaaioaaeaiiy ca oiia o?ii?/iiai
aeine?aeo neeaaeaei 25 nA?, aac o?aooaaiiy u?eueii?ii?coth/i? eiiiiiaioe
a?ae o?aino?aiiaeo aeaiaio?a.

A aeine?aeao caaacia/oaaee noeioeyoe?th a?ineioaco
i?eioeiai?aeaaeai?i-aeeioeeaioeaeo ( IAAe ) iaeii?aciaei
aioo??oiuei/a?aaiei aaaaeaiiyi ?ioaeoiei oaa?eiai 20 ia i?eioeiai?aeo ia
100 a iane o?ea.

Oeion eiio?ieueieo oa ii?ii?iaieo oaa?ei ia?aoe?aee /a?ac 4 oa?e
iaeeiiiai? n?oee o na?aaeiaeu? Oaiena, aeae? io?eioaaee noniaic?th
oeiioeeo?a ( Oiaineee, 1986 ). O?aaiaioaoe?th AeIE, oa?aeoa?io aeey
aiiioico, aeyaeyee ca aeiiiiiaith aeaeo?ioi?aco a 1,2 % aaa?iciiio aae?,
aeaa?aaeaoe?th AeIE aecia/aee iaoiaeii Nieia”yia, ( 1993 ). Aeey
aeyaeaiiy iaeii – oa aeaiieoeiaeo iioeiaeaeaiue AeIE ( I?, Ae? ) a
noniaic?? oeiioeeo?a aeei?enoiaoaaee iaoiae Aeieo?aiea, ( 1997 ).
Aecia/aee iicaieaiiaee neioac AeIE ( Nieoeianeee, 1992 ). I?iaiaeeee
aeine?aeaeaiiy iaeo?aeueii? i?ioaie?oe/ii? aeoeaiino? yaea?iiai iao?eeno
( Iaeaoiaa, 1990 ) oa aeoeaiino? eaoainei A-iiae?aieo i?ioa?iac
( *i?ia, 1996 ). Aeine?aeaeoaaee noai ia?aeeniiai ieeneaiiy e?i?ae?a (
Noaeueiay, Aa?eoaeee, 1977 ). Aecia/aiiy Na2+, Ig2+-caeaaeiiai oa
Ig2+-caeaaeiiai iaeiie/aiiy iie?-aeacieneioeeaioeae?a ( IAeI ) o
oeiioeeoao ii?ii?iaieo uo??a i?iaiaeeee ca iaoiaeii Oiainueeiai, ( 1988
). Aecia/aiiy a?eea i?iaiaeeee ca iaoiaeii Lowry, ( 1951 ). Noaoenoe/io
ia?iaeo ?acoeueoao?a cae?enithaaee c aeei?enoaiiyi i?ia?ai Microsoft
Excel.

?ACOEUeOAOE OA ?O IAAIAI?AIIss

I?e ae?? aeno?aiaeueieo oaeoi??a ia ee?oeio aeieea? aaiiiia oa
aiiainoacia ianoaa?euei?noue ( Eoiao, 1995 ). Iayai?noue
aioo??oiueiee?oeiieo iaoai?ci?a, ye? iiaeooue caaacia/eoe aaiiii? ?
iaoaaie?/i? ia?aaoaeiae, ia? ia o?eueee aaiethoe?eia, a e
aaciina?aaeiuei aaeaioeaia cia/aiiy ( Nieiaueyi, 1990 ). Oaeee noai
i?aai?cio aeioe?eueii cia?aceoe noaiith: no?an -aaeaioaoe?y -aeaeeaaiiy
aai nia?oue.

I?e aeno?aiaeuei?e ae?? no?aoaa?y i?aai?cio iai?aaeaia ia oa, ui
ee?oeie, ye? a?i ia iiaea a?aeiiaeoe ? eiio?iethaaoe, i?aeeyaathoue
caaeaae? oeyoii aeeth/aiiy i?ia?aie aiiioico.

Iniiaiei ? caaaeueiia?aeiiei a?io?i?/iei ia?ea?ii aiiioico a naieo
??cieo ee?oeiao ? i?ae ae??th ??cieo noeioe?a ? oii?yaeeiaaia
i?aeioeeainiiia aeaa?aaeaoe?y

5

o?iiaoeio. Oeae i?ioean ? ia?oith iacai?ioiith iiae??th, ui aecia/a?
aeieth ee?oeie. Iaeiei ?c eiai i?iya?a ? iaeiie/aiiy ?ic/eiieo
aeacieneioeeaii?ioa?ae?a ( AeII ).

?. O?aaiaioaoe?y AeIE oeiioeeo?a ca oiia i?iiaiaaiai o?aaeaiiy

Caeiiii??iino? aeaa?aaeaoe?? o?iiaoeio oeiioeeo?a ii?ii?iaieo uo??a.
A?aeiii, ui iniiaieie iane?aeeaie ae?? ?ii?coth/i? ?aae?aoe?? ia ee?oeie
?aae?i/ooeeaeo i?aai?a ? ?iciaae o?iiaoeio ? iaeiie/aiiy
iie?aeacieneioeeaioeae?a. Iaeiae a iaoeia?e e?oa?aoo?? iaiaeue aeaieo
i?i oa?aeoa? oa caeiiii??iino? aeaa?aaeaoe?? o?iiaoeio ? iniaeeaino?
ii/aoeiaeo iaoai?ci?a oeeo i?ioean?a. ?niothoue oaeiae noia?a/eea? aeai?
i?i aeiciao caeaaei?noue, ?icaeoie oeeo iiae?e a /an?, ?ioaineai?noue
?iciaaeo a oiiaao ae?? ?ii?coth/i? ?aae?aoe?? ??cii? ?ioaineaiino?.

Oiio ia ia?oiio aoai? ?iaioe ie aeine?aeaeoaaee oa?aeoa? oa iniiai?
caeiiii??iino? aeaa?aaeaoe?? o?iiaoeio oeiioeeo?a ii?ii?iaieo uo??a i?e
ae?? ??cieo aeic ?aae?aoe??, a oaeiae ii??aithaaee ae?th iaeii?aciaiai
?aioaai?anueeiai ii?ii?iaiiy ia uo??a c iane?aeeii ae?? o?ii?/iiai
?ii?coth/iai ii?ii?iaiiy iecueei? ?ioaineaiino?.

I?e aeei?enoaii? aeaeo?ioi?aco a aaa?iciiio aae? ia aaea?oueny
niinoa??aaoe

/?oei? ea?oeie o?aaiaioaoe?? o?iiaoeio oeiioeeo?a /a?ac 3 aiae i?ney
ae?? ia oaa?ei aei?ii?ithaaiiy a aeic? 0.5 A?, aea a?aei?/a?oueny eiai
aeaa?aaeaoe?y. *a?ac 1oa 2 aiae i?ney ae?? aei?ii?ithaaiiy a aeic? 1 A?
aoei aeyaeaii iineeaiiy aeaa?aaeaoe?? o?iiaoeio, aea a?aenooi? /?oei
noi?iiaai? aeene?aoi? iaeano? AeIE. sse aeaeii c ?en. 1, iiyaa iaai?o
AeIE-o?aaiaio?a a?aeaoaa?oueny /a?ac 3 aiae i?ney ae?? ia uo??a
?ii?coth/i? ?aae?aoe?? a aeic? 1 A?. Ioeeainiii? o?aaiaioe oi?iothoue
aeene?aoi? iaeano? ca oeiii “ae?aaeie” a?aeiia?aeii aei ?o ?ici??o. I?e
oeueiio ia aeaeo?ioi?aa?ai? a aaa?iciiio aae? aeyaeythoueny ae?aci?
nioae, ui, ye i?aaeei, a?aeiia?aeathoue o?aaiaioai AeIE aeiaaeeiith 200,
600 oa 800 ia? ioeeaioeae?a ( Iaoeoaanueea, 1997 ).

I?e ii?ii?iaii? a aeic? 2 oa 4 A? niinoa??aa?oueny iaeiie/aiiy
aeene?aoieo AeIE-o?aaiaio?a. E?euee?noue o?aaiaio?a ia ca?eueoo?oueny c
i?aeaeuaiiyi aeice. Ca?eueoaiiy aeice aei 8 A? i?eaiaeeoue aei
i?aeaeuaiiy ai?noo AeIE a oa?i?iaeueieo ciiao aeaeo?ioi?aa?aie.
Ia?aeaeueii a?aeaoaa?oueny oi?ioaaiiy iiaeo o?aaiaio?a AeIE, ui iathoue
a?eueoo iieaeoey?io iano.

I?e aea/aii? no?oeoo?iiai noaio o?iiaoeio oeiioeeo?a o?ii?/ii
ii?ii?iaieo uo??a anoaiiaeaii, ui a?aeaoaa?oueny eiai o?aaiaioaoe?y.
E?euee?noue

7

o?aaiaio?a AeIE cia/ii c?inoa? oa ?iciiae?eai? aiie ??aiii??i?oa ca
niaeo?ii iieaeoey?ieo ian ( ?en. 2 ).

A ?acoeueoao? i?iaaaeaieo aeine?aeaeaiue anoaiiaeaii, ui ae?y o?ii?/iiai
?ii?coth/iai aei?ii?ithaaiiy a aeic? 25 nA?, ye ? iaeii?aciaiai
?aioaai?anueeiai ii?ii?iaiiy, i?eaiaeeoue aei aeoeaaoe?? i?ioean?a
o?aaiaioaoe?? AeIE a oeiioeeoao c iiae?aiei oa?aeoa?ii.

Ae?oaei ?c i?aeoiae?a aeey aecia/aiiy nooiaiy aeaa?aaeaoe?? o?iiaoeio a
ee?oeiao aoei aeine?aeaeaiiy aeoiaeo iecueei-iieaeoey?ieo o?aaiaio?a
AeIE – IAeI. Iaie anoaiiaeaii, ui c i?aeaeuaiiyi aeice aei?ii?ithaaiiy
niinoa??aa?oueny aeinoia??ia ca?eueoaiiy iaeiie/aiiy IAeI ( ?en. 3 ).

A ?acoeueoao? iaeiie/aiiy IAeI, ye? iathoue ??cio iieaeoey?io iano,
a?aeaoaa?oueny oi?ioaaiiy aeene?aoieo iaeanoae ia aeaeo?ioi?aa?aiao.
Oaeo ?o iiyae aea? ciiao c?iaeoe aeniiaie i?i aeoeaaoe?th a ee?oei?
i?ioean?a, ye? aaea ia i?aeeyaathoue eiio?ieth c aieo ?aia?aoe?eii?
nenoaie. Io?eiai? iaie aeai? oa ?acoeueoaoe e?oa?aoo?e ( Nieaeaoaieia,
1989 ) aeicaieythoue noaa?aeaeoaaoe, ui a ii?ii?iaieo e?ioioeeoao
aeaa?aaeaoe?y AeIE ? ?aiiueith iiae??th ? ia?aaeo? caaeaae? oeeo ee?oei.

Ooai?aiiy ?ic?ea?a AeIE oa ?o ?aia?aoe?y. Aeey aiae?co o?aaiaioaoe??
AeIE oeiioeeo?a ii?ii?iaieo uo??a ie aea/aee aeeiai?eo no?oeoo?ieo ci?i
o?iiaoeio. Oe?e?ni?noue iie?ia?ii? AeIE i?ney ii?ii?iaiiy ii?ooo?oueny
aiane?aeie ooai?aiiy iaeii-ieoeiaeo oa aeaiieoeiaeo ?ic?ea?a. sse aeaeii
c ?en. 4, i?ioean ?icuaieaiiy no?oeoo?e AeIE ia? oaciaee oa?aeoa?. Oae,
aaea /a?ac 15 oa i?ney ii?ii?iaiiy a?aeaoaa?oueny ca?eueoaiiy e?eueeino?
iaeiiieoeiaeo ?ic?ea?a, ui ia aeae?

8

iiaea aecia/aoe ?icaeoie iiae?e. Oi/a a?eueo?noue ?o e?ea?aeothoueny,
aeayea /anoeia caeeoa?oueny ia?aia?iaaiith ? i?eaiaeeoue aei aeieeiaiiy
aeiaeaoeiaeo aeaiieoeiaeo ?ic?ea?a, e?euee?noue yeeo /a?ac 120 oa.
ii/eia? ca?eueooaaoenue. E?eoe/iee ??aaiue aeaiieoeiaeo ?ic?ea?a,
iiaeeeai, ? neaiaeii a ?acoeueoao? yeiai a?aeaoaa?oueny aeoeaaoe?y
nenoai aeaa?aaeaoe?? aaiaoe/iiai iaoa??aeo ( Bruant, 1984 ).

O a?aeiia?aeue ia ae?th aei?i-i?ithaaiiy a?aeaoaa?oueny aeoeaaoe?y
ooieoe?iioaaiiy ?aia?aoe?eieo nenoai, ye? iathoue cia/iee, aea iaaiee
?aca?a. ( ?ya/aiei, 1982, Shall, 1982 ). Oea i?eaiaeeoue aei oiai, ui
i?eaeecii /a?ac 2 aiae e?euee?noue iaeiiieoeiaeo ?ic?ea?a ciaioo?oueny.
?iaeaen ?aia?aoe?? ca?eueoo?oueny ii??aiyii c ia?oei aeine?aeaeaiei
oa?i?iii. Oa a ?ioa?aae? aei 60 oa iicaieaiiaee neioac AeIE i?aeaeuaiee
noiniaii ?ioaeoieo oaa?ei ( ?en. 5 ).

C i?eaaaeaieo aeua aeaieo aeaa?aaeaoe?? ? ?aia?aoe?? iiaeia c?iaeoe
aeniiaie i?i oa, ui ia ia?oiio aoai? ( 120 oa ) a ?acoeueoao? ae??
?ii?coth/i? ?aae?aoe?? a?aeaoaa?oueny iaeiie/aiiy aeaiieoeiaeo ?ic?ea?a
AeIE. A ca?eueoaiiy e?eueeino? iaeiiieoeiaeo ?ic?ea?a AeIE i?eaiaeeoue
aei ciaioaiiy neioaoe/iiai iioaioe?aeo ee?oeie.

O?ii?/ii ii?ii?iai? oaa?eie iaee ?ioee ?iciiae?e aeine?aeaeaieo
iieaciee?a. Oae, i?e aiae?c? iaeii – oa aeaiieoeiaeo ?ic?ea?a AeIE ?o
e?euee?noue a?aeiia?aea? cae?i/aiith ia?oi? oace aeaa?aaeaoe??
aaiaoe/iiai iaoa??aeo. Aea ?iaeaen ?aia?aoe?? ia?aaeuo? ii?io, ui iiaea
na?ae/eoe i?i iioaioe?eio aeeoo?caeaoi?noue ee?oei. I?eeiath/e aei oaaae
?ai?oa io?eiai? aeai? ca aeiiiiiaith aeaeo?ioi?aco: a?eueoo, o
ii??aiyii? c iaeii?aciaei ?aioaai?anueeei ii?ii?iaiiyi, e?euee?noue
aeene?aoieo cii aeaa?aaeaoe?? o?iiaoeio, ?o ??aiii??ia ?iciiae?eaiiy ca
niaeo?ii iieaeoey?ieo ian, cieeiaiiy cacia/aieo iniaeeainoae /a?ac 2-3
oeaei? i?ney ii?ii?iaiiy oaa?ei iiaeia c?iaeoe aeniiaie, ui oe? ee?oeie
ciaoiaeyoueny o noai? aaeaioaoe??.

?acoeueoaoe ii??aiyiiy ae?? iaeii?aciaiai ?aioaai?anueeiai oa o?ii?/iiai
aei?ii?ithaaiiy na?ae/aoue i?i oa, ui ciaioaiiy ?iaeaeno ?aia?aoe?? ia
iia’ycaii c? ciaioaiiyi ?ano?no ee?oeie, a ? iane?aeeii ae?? i?ia?aie
aaeaioaoe?? a?eueo neeaaeii? ca ???a?o??th a?ieia?/ii? nenoaie.

9

Iaoai?ci ?icaeoeo o?aaiaioaoe?? o?iiaoeio oeiioeeo?a ii?ii?iaieo uo??a.

Aenia?eiaioaeueiei oeyoii aoei anoaiiaeaii, ui Na2+, Mg2+-caeaaeia
aiaeiioeeaaca cae?enith? ?ic?eae ieoie AeIE, ui ciaoiaeyoueny i?ae
ioeeainiiaie ( Cohen, 1984 ). Aiane?aeie oeueiai c’yaeythoueny ?ic?eae,
ye? ianooue 3’-II e?ioe? ( Aaeaoeeee, 1987 ), iaeiie/othoueny IAeI. Oiio
/anoeia iaoi? ?iaioe aoea iai?aaeaia ia aea/aiiy oa?iaioaoeaii?
aeaa?aaeaoe?? AeIE oa aecia/aiiy i?e?iaee ioeeaac, ui a?aeiia?aeathoue
ca oeth aeaa?aaeaoe?th a oeiioeeoao ii?ii?iaieo uo??a. *a?ac 30 oa i?ney
ae?? ?aae?aoe?? a oeiioeeoao ii/eia? ca?eueooaaoenue Na2+, Mg2+-caeaaeia
iaeiie/aiiy IAeI, ui /a?ac 120 oa iaaoaa? iaeneiaeueiiai cia/aiiy ( i?e
oeueiio aiii ca?eueoo?oueny a i’youe ?ac?a o ii??aiyii? c eiio?ieai ).
?aia?aoe?ei? nenoaie aaea ia ni?aaeythoueny c iiaith oaeeath ooai?aiiy
oa?iaioaoeaieo ?ic?ea?a, ? /a?ac 180 oa niinoa??aaeanue aii?yaeeiaaia
aeaa?aaeaoe?y AeIE. Aeae? a?aeaoaa?oueny ae?i?iaoe?y ee?oei c
aeaa?aaeiaaiei o?iiaoeiii.

Iaie aoei aeine?aeaeaii ? Mg2+-caeaaeia iaeiie/aiiy IAeI, ine?eueee
Mg2+-caeaaeia ioeeaaca a?aeiia ye oa?iaio, ui oaeiae aeaa?aaeo? iaoeaio
AeIE ( Ieeiiiaa, 1982 ). sse aeaeii c ?en. 6, /a?ac 120 oa.
Mg2+-caeaaeia iaeiie/aiiy IAeI ca?eueoo?oueny o aeaa ?ace, aea o
ii??aiyii? c aeoeai?noth Na2+, Mg2+-caeaaeii? aiaeiioeeaace iiaeia
c?iaeoe aeniiaie i?i iacia/iee aeeaae oeueiai oa?iaioo o oi?ioaaiiy
aoaeoo.

Oaeei /eiii, iiaeia cacia/eoe, ui a oiiaao iaoiai aenia?eiaioo 120 oa
i?ney ae?? ii?ii?iaiiy iiaeia aaaaeaoe iacai?ioiei oa?i?iii i?ioeano
iioeiaeaeaiiy ee?oei. Na2+, Mg2+-caeaaeia aiaeiioeeaaca -?i?oe?th?
i?ioean oa?iaioaoeaiiai ?iciaaeo o?iiaoeio, ? ?? oa?iaioaoeaia
aeoeai?noue aecia/a? o?aaiaioaoe?th aaiaoe/iiai iaoa??aeo. Neaiaeii aeey
ca?eueoaiiy aeoeaiino? oa?iaioo iiaea aooe ca?eueoaiiy ia?aia?iaaieo
aeaiieoeiaeo ?ic?ea?a.

??. Noai IIE oeiioeeo?a i?e ae?? ?aioaai?anueeiai ii?ii?iaiiy

A oeiioeeoao i?ney ii?ii?iaiiy oaeaeei i?enei?ththoueny
a?eueii?aaeeeaeuei? ?aaeoe?? ia?aeeniiai ieeneaiiy, ui
noi?iaiaeaeothoueny ?icuaieaiiyi neeaaeieo

10

i?aai?/ieo niieoe, aeeneieoaoe??th ?o aia?aaoe/iiai iioaioe?aeo ?
ooai?aiiyi oiene/ieo i?iaeoeo?a e?iiia?aeenii? i?e?iaee ( Eoae?yoia,
1961; Na?e?c, 1990; Eoae?yoia, 1992 ). Oiio ianooiiei aoaiii iaoeo
aeine?aeaeaiue aoei aea/aiiy aeeiai?ee niiioaiiiai, oa?iaioaoeaiiai oa
iaoa?iaioaoeaiiai ieeneaiiy a oeiioeeoao ii?ii?iaieo uo??a.

Oa?iaioaoeaia nenoaia IIE aeei?enoiao? aeiii?ii a?aeiiaeaieo
aea?aaeaio?a IAAeOI, a iaoa?iaioaoeaia-anei?a?iiao eeneioo. Niiioaiia
ia?aeenia ieeneaiiy aei??thaaee i?e ?ieoaaoe?? noniaic?? ee?oei aac
aeiaeaaaiiy a?aeiiaiee?a.

Oaae.7. Iaeiie/aiiy iaeiiiaiai ae?aeueaeaa?aeo a oeiioeeoao uo??a i?e
ii?ii?iaii? a aeic? 1 A? ( iiieue IAeA ia 2,5 o 107 ee., I ± m )

Iaeiie/aiiy IAeAEiio?ieue15 oaeeei120 oaeeei180
oaeeeiniiioaiia192±28307±35*384±41*769±101*anei?aaocaeaaeia500±43577±21*
961±67*641±48*IAAeOI-caeaaeia288±25512±38*1250±84*404±35** – ?aaeaioaoeey->auaeeaaiea eee nia?oue. Ia noaaeee aaeaioaoeee
aaeain eiiiainaoi?iuo, caueoii-ainnoaiiaeoaeueiuo e iaoaei-ae/aneeo
?aaeoeee ii?aaeaeyao iaeei ec aiciiaeiuo iooae iinono?anni?iiai ?acaeoey
eeaoee. I?e yoii no?aoaaey i?aaiecea iai?aaeaia ia yeeieiaoeeth eeaoie,
eioi?ua ii ia iiaeao ainnoaiiaeoue e eiio?iee?iaaoue iooai aeeth/aiey
i?ia?aiiu aiiioica. A ?acoeueoaoa i?ienoiaeeo

18

naeaeoeey eeaoi/iuo iiioeyoeee. Oaeay aeeiaie/iinoue eiioiiie nenoaiu
aeaao aiciiaeiinoue i?aaiecio aaeaioe?iaaoueny e iiaeaea?aeeaaoue
iioeiaeueiia ninoiyiea a iinoiyiii eciaiythueony aiaoieo oneiaeyo.

Eniieueciaaiea ieeioeiaieaea a oeceieiae/aneeo eiioeaio?aoeeyo, eae
?aaeeiiiaeeoeoee?othuaai i?aia?aoa, i?eaiaeeo e aeoeaaoeee aeaa?aaeaoeee
o?iiaoeia oeiioeeoia iaeo/aiiuo e?un. Aaaaeaiea ieeioeiaieaea
niinianoaiaaei oiaiueoaieth iaeiiieoaauo ?ac?uaia ii i?e yoii eiee/anoai
aeaoieoaauo ?ac?uaia aic?anoaao. I?e yoii iaaethaeaaony iiauoaiea
aeoeaiinoe Na2+, Mg2+-caaeneiie yiaeiioeeaacu a ia/aeueiue ia?eiae
aeaa?aaeaoeee o?iiaoeia. Oaeie yooaeo iiaeii iaynieoue oai, /oi
ieeioeiaieae, a iaoeo oneiaeyo yenia?eiaioa, i?iyaeyao naay eae
eiaeaeoi? AAeO-?eaiceee?iaaiey. Yoi i?eaiaeeo e oiaiueoaieth a?aiaie
aeaa?aaeaoeee aaiaoe/aneiai iaoa?eaea a oeiioeeoao iaeo/aiiuo aeeaioiuo.

Eeth/aaua neiaa: oeiioeeou, ?aioaaiianeia eceo/aiea, o?iiaoei,
iaeiiieoeaua e aeaoieoeaua ?ac?uau, i?ioaeiacu, ieeioeiaieae.

Parhomets Yu.P. Consistent patterns of degradation of chromatin
thymocites under radiation exposition of rats.- Manuscript.

Grace thesis for scientific degree Ph.D in biology with the
spesialization 03.00.01 – radiobiology. Tarasa Shevchenko Kyiv
University, Kyiv, 1999

The thesis is devoted to research of the all-out effect of X-ray and
chronic emanation of low activity on degradation of the structure of the
thymocites chromatin of rats. It was detected that fragmentation by
“lesenka” type of thymocites chromatin of rats is started in 3 hours
after one time X-ray emanation in doze of 1Gr. The influence of
combined chronic emanation in doze 25cGr. leads to the similar type
chromatin degradation. The dynamic of one chain and double chain break,
reparation processes, lipids peroxidation, proteinase activity in
thymocites of X-rayed rats. The use of nicotinamid as a radiomodeling
agent, leads to the activation of the process of degradation chromatin.

Key words: thimocytes, X-ray, chromatin, one and double break,
proteinases, nicotinamide

I?aeienaii aei ae?oeo 17.05.99?. Oi?iao 60o90/16

Oi. ae?oe. a?e. 1,0. Iae.-aeae.a?e.1,0

Iaeeaae 100. Cai.214.

i.Ee?a-5, aoe.*a?aiiia?i?enueea,57/3,e.201.

Oiaa?enoai “Ciaiiy” Oe?a?ie

Aeaeaaieoeoai oa iia?aoeaia iie?a?ao?y

227-41-23, 294-71-27

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