CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue i?iaeaie. C?inoaiiy e?eueeino? caoai?thaaiue,
ao?ieia?/iei oaeoi?ii yeeo ? Staphylococcus aureus neeaaea? iaeio ?c
aeooaeueieo i?iaeai i?e?ia?ieia?? oa ee?i?/ii? iaaeeoeeie. Iniaeeai?
aino?ioe iaaoaa? oey i?iaeaia a ca`yceo ?c ?iciianthaeaeaiiyi
aioea?ioeei?acenoaioieo ooai?a, ui caaaea? o?oaeiiu?a i?e i?aeai??
e?ea?nueeeo cania?a aeey ai?ioueae ?c aeaiith ?ioaeoe??th. A inoaii?e
/an, ii?yae ?c aenieith no?ee?noth aei aioea?ioee?a, ye?
aeei?enoiaoaaeeny ?ai?oa, oaeaeei a?aeaoaa?oueny i?enoinoaaiiy ? aei
iiaeo i?aia?ao?a, ui ai?iaaaeaeothoueny a e?eoaaeueio i?aeoeeo.

Oe?iea iioe?aiiy ?acenoaioieo ooai?a noao?eieiea, ca aeoieith a?eueoino?
aaoi??a, iia,ycaia ?c aeoaea ieanoe/iei iai?iii ?a/iaei, ui aea?
iiaeeea?noue aeey iiyae iaoaaie?/ieo ?aaeoe?e, ye? eaaeaoue a iniia?
a?io?i?/ieo iaoai?ci?a ?acenoaioiino?. C ae?oaiai aieo, aaiaoe/i?
aeaoa?i?iaioe, ui eiaeothoue aioea?ioeeino?ee?noue a oiae? i?ioean?a
aaiaoe/ii? ?aeiia?iaoe?? aeoaea oaeaeei iioe?ththoueny a iiioeyoe?yo
noao?eieie?a.

Iaoai?cie no?eeino? i?e?ii?aai?ci?a aeey aaaaoueio aioea?ioee?a
?icoeo?iaai?. Oaiioei?/iee i?iya no?eeino? iiaea aooe iia,ycaiee ?c
neioacii oa?iaio?a, ui ?iaeoeaothoue aioea?ioee, ?c ii?ooaiiyi
i?iieeeeaino? iiaa?oiaaeo no?oeoo?, ui caiia?aa? noai?aiith
aaeoa??inoaoe/ieo eiioeaio?aoe?e i?aia?aoa ana?aaeei? ee?oeie,
iiaeeo?eaoe??th i?oai? ae?? aioea?ioeea oa aeieeiaiith iao?aeieo
iaoaaie?/ieo oeyo?a. E??i oiai, ye iieaco? aiae?c e?oa?aoo?ieo aeaieo,
?acenoaioi?noue aei ?yaeo aioea?ioee?a iiaea aooe iia,ycaia iaeii/anii
?c ?icaeoeii aeae?eueeio a?io?i?/ieo iaoai?ci?a no?eeino?.

Ana oea na?ae/eoue i?i oa, ui no?ee?noue aei aioea?ioee?a ? neeaaeiei
a?ieia?/iei yaeuai, yea iia,ycaii c iaeiei ?c iai?yie?a aaiethoe??
iaoiaaiieo aaeoa??e oa oi?ea?oueny anueiai iaoaaie?cio i?e?iaii?
ee?oeie. Oiio iaiao?aeii eiiieaenia ii??aiyeueia aea/aiiy ?aaeoe?e
aia?aaoe/iiai oa eiino?oeoeaiiai iai?i?a o /ooeeaiai oa no?eeeo aei
aioea?ioee?a ooai?a noao?eieiea c iaoith iiooeo caaaeueieo
caeiiii??iinoae oa oeyo?a ?icaeoeo a?io?i?/ieo iaoai?ci?a
?acenoaioiino?, iaoai?ci?a aaiaoe/ii? aeaoa?i?iaoe?? oaiioei?/iiai
i?iyao no?eeino?, a oaeiae iiaeeeaeo oeyo?a ia?aaea/? aaiaoe/ieo
aeaoa?i?iaio a iiioeyoe?? noao?eieie?a.

Iaoa ? caaaeaiiy aeine?aeaeaiue. A ca,yceo ?c aeeeaaeaiei, iaoith ?iaioe
aoa ii??aiyeueiee aiae?c ?ioaineaiino? ?aaeoe?e aia?aaoe/iiai oa
eiino?oeoeaiiai iai?i?a o /ooeeaiai ooaio noao?eieiea oa ?yaeo eiai
aa??aio?a, ye? no?ee? aei oao?aoeeee?io, oei?aioai?eieo, a?eo?ii?oeeio,
iai?oeee?io oa ooceaeeio.

Aeey aeeiiaiiy ?iaioe iaiao?aeii aoei ae??oeoe oae? caaea/?:

1. Aea/eoe iniaeeaino? ooieoe?iioaaiiy iniiaieo eaoaaie?/ieo oeeee?a
ooai?a noao?eieiea a ca,yceo c no?ee?noth aei aioea?ioee?a.

2. I?iaanoe ii??aiyeueia aea/aiiy ieenethaaeueieo i?ioean?a o /ooeeaiai
oa ?acenoaioieo ooai?a noao?eieiea.

3. Aeine?aeeoe iniiai? ?aaeoe?? a?ineioaco a?ee?a, ioeea?iiaeo eeneio oa
e?i?ae?a o no?eeeo aei aioea?ioee?a noao?eieie?a.

Iaoeiaa iiaecia ?iaioe. Aia?oa iieacaii, ui no?ee?noue aei ?yaeo ??cieo
ii iaoai?cio ae?? ia aaeoa??aeueio ee?oeio aioea?ioee?a o noao?eieiea
noi?iaiaeaeo?oueny caaaeueieie caeiiii??iinoyie ia?aaoaeiae
aia?aaoe/iiai oa eiino?oeoeaiiai iai?i?a.

Anoaiiaeaii ci?io ni?aa?aeiioaiiy iniiaieo eaoaaie?/ieo oeeee?a o
?acenoaioieo aa??aio?a noao?eieiea a ii??aiyii? ?c /ooeeaei ooaiii.
Iieacaii iineeaiiy ?ie? ae?eie?co ia oii? cieaeaiiy aeoeaiino?
iaioicioinoaoiiai oeeeeo a eaoaaie?ci? aoaeaaiae?a.

Aeyaeaii ciaioaiiy oaeaeeino? ieeneaiiy i??oaaoo oa aoeaoaoo no?eeeie
ooaiaie, ui na?ae/eoue i?i cieaeaiiy ieenethaaeueii? aeoeaiino? OeOE oa
a?eueo ?ioaineaiee a?aeoie ?ioa?iaae?ao?a oeeeeo ia a?ineioaoe/i?
i?ioeane.

No?ee?noue aei aioea?ioee?a, ye? aea/aeenue noi?iaiaeaeo?oueny
iineeaiiyi neioaco iaea?eueo aaaeeeaeo aeey ee?oeie
a?iiie?ia??a-ioeea?iiaeo eeneio, a?ee?a, e?i?ae?a.

I?aeoe/ia cia/aiiy ?iaioe. Io?eiai? ?acoeueoaoe iiaeooue aooe
aeei?enoai? aeey neeaaeaiiy a?eueo iiaii? eiiieaenii? oa?aeoa?enoeee
iaoai?ci?a no?eeino? aei aioea?ioee?a, ui a naith /a?ao cia/ii
iieaaoeoue i?aea?? e?ea?nueeeo cania?a aeey ai?ioueae c iaoiaaiieie
noao?eieieaie oa aeanoue iniiao aeey ?ic?iaee noai ?aoe?iiaeueii?
aioea?ioeeioa?ai?? caoai?thaaiue noao?eieieiai? ao?ieia??.

Ca,ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. I?aaenoaaeai?
aeine?aeaeaiiy-oea /anoeia ?iaioe, ui i?iaiaeeoueny ia eaoaae??
i?e?ia?ieia?? oa a??onieia?? Aei?i?iiao?ianueeiai aea?aeaaiiai
oi?aa?neoaoo a ?aieao aea?aeathaeaeaoii? oaie ?01-11-97 “Aeine?aeaeaiiy
iniaeeainoae iaoaaie?/ieo i?ioean?a o i?e?ii?aai?ci?a oa ?ic?iaea
oaoi?/ieo oiia ae?iaieoeoaa iiaeo a?ieia?/ii-aeoeaieo i?aia?ao?a”, yea
eii?aeeio?oueny ieaiii iaoeiai-aeine?aeieo oai I?i?noa?noaa ina?oe
Oe?a?ie.

Iniaenoee aianie caeiaoaa/a. ?iaioa aeeiiaia iniaenoi aaoi?ii i?ae
ea??aieoeoaii aeieo.a?ie.iaoe, i?ioani?a A.?.A?ii?eiaa. Iniaenoa o/anoue
aeena?oaioa iieyaaea o i?iaaaeaii? eaai?aoi?ieo aeine?aeaeaiue, aiae?c?,
ocaaaeueiaii? iaea?aeaieo aenia?eiaioaeueieo aeaieo, ni?anoaaeaii? ?o c
e?oa?aoo?ieie iaoa??aeaie. Aei??thaaiiy aeoeaiino? oa?iaio?a i?iaaaeaii
ni?eueii c eaiae.a?ie.iaoe,aeioe. A.A.Aaa?eethe oa
eaiae.a?ie.iaoe,aeioe. E.I.Aieiaeie. Aaoi? i?eiineoue ue?o aaey/i?noue
ni?a?ia?oieeai eaoaae?e a?io?i?? Ee?anueeiai iaoe?iiaeueiiai
oi?aa?neoaoo aeieo.a?ie.iaoe,i?io. A.I. Aieoeeoeueeiio oa
aeieo.a?ie.iaoe,aeioe. N.A.Oeaeiye ca aeiiiiiao a i?iaaaeaii?
aenia?eiaioo ii aeeth/aiith i?/aieo niieoe a ee?oeie noao?eieie?a.

Ai?iaaoe?y ?iaioe. Iaoa??aee aeena?oaoe?eii? ?iaioe aoee i?aaenoaaeai?
ia I I?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe?? /Aei?i?iiao?ianuee
1995/; II Oe?a?inueeiio ?aae?ia?ieia?/iiio c`?cae? /Aei?i?iiao?ianuee,
1995/; Eaenoiinueeiio neiiiceoi? c iieaeoey?ii? oa ee?oeiii? a?ieia??
/NOA,1996, 1997/; VII c,?cae? Oe?a?inueeiai a?io?i?/iiai oiaa?enoaa
/Ee?a,1997/; II Oe?a?inueeiio a?io?ce/iiio c,?cae? /Oa?e?a, 1998/; I
I?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe?? “Iaoea oa ina?oa”
/Aei?i?iiao?ianuee, 1998/, VII I?aeia?iaei?e eiioa?aioe?? “Aeueyin
O?ainac” (Aei?i?iiao?ianuee, 1998).

Ioae?eaoe??. Ii oai? aeena?oaoe?? iioae?eiaaii 8 iaoeiaeo ?ia?o.

no?oeoo?a oa ia’?i aeena?oaoe??. Aeena?oaoe?eia ?iaioa neeaaea?oueny
c anooio, iaeyaeo e?oa?aoo?e, aenia?eiaioaeueii? /anoeie, iaaiai?aiiy,
aeniiae?a ? nieneo oeeoiaaii? e?oa?aoo?e, yeee aeeth/a? 227
iaeiaioaaiue. ?iaioa aeeeaaeaia ia 140 noi??ieao iaoeiiieniiai oaenoo,
?ethno?iaaia 17 ?enoieaie oa 19 oaaeeoeyie.

CI?NO ?IAIOE

Iaeyae e?oa?aoo?e

Iaeyae e?oa?aoo?e neeaaea?oueny ?c 3 ?icae?e?a, a yeeo aena?oeai?
ieoaiiy eaoaaie?/iiai iai?io ? aeoioeyoe?? aia?a?? o noao?eieie?a,
ioa?aeoa?eciaai? iniaeeaino? a?ineioaco iniiaieo eean?a niieoe – a?ee?a,
ioeea?iiaeo eeneio, e?i?ae?a oa aoaeaaiae?a, a oaeiae i?eaaaeai? iniiai?
a?io?i?/i? iaoai?cie no?eeino? aei ?yaeo iaea?eueo aaaeeeaeo
aioea?ioee?a. Iieacaii, ui i?ioeane iaoaaie?cio o noao?eieie?a aea/ai?
iaaeinoaoiuei ? oiio iaiao?aeia iiaeaeueoa a?eueo aeaoaeueia aea/aiiy ye
ie?aieo aia?aaoe/ieo oa a?ineioaoe/ieo ?aaeoe?e, oae ? iaoaaie?cio a
oe?eiio, ui ni?eyoeia a?eueo aoaeoeai?e ai?iouea? ?c aeaiith
?ioaeoe??th.

iaoa??aee ? iaoiaee aeine?aeaeaiue

Ia,?eoii aeine?aeaeaiue aoee ooaie Staphylococcus aureus: 8325-4 oa eiai
aa??aioe, ye? i?noyoue ieaci?aee ?acenoaioiino? aei ??cieo aioea?ioee?a.
Ooaie aoee i?aaeinoaaeaii c eieaeoei? iinoeoooo aiiaeaiiieiai? oa
iie?iaiieiai? ii. Ae.E.Aaiaea? AII ?ini?.

Aeey iaea?aeaiiy noniaici? «nii/eaath/eo» eeioei i?iaiaeeee iaeiie/aiiy
aiiiane ooai?a noaoieieiea ia oaa?aeiio aeeaeeueiiio na?aaeiaeui, ui
iinoeoue IIA, ?I 7,0-7,2 i?e t =37i N aei na?aaeeie eiaa?eoii/ii? oace
?inoo. Aeey iaea?aeaiiy aaceeioeiiiai aiiiaaiaoo ?oeioaaiiy eeioei
noaoieieiea i?iaiaeeee ia oeueo?acaoeiaiio aeaceioaa?aoi?i OCAeI-1 c
aeniiiaioeiaeueiei aei?iiiithaa/ai. Icao/oaaiiy i?iaiaeeee ia i?ioyci 10
oaeeei i?a?eaenoi c iioa?aaeii a 30 nae. Iac?oeiiaai? eeioeie oa
eeioeiii noiiee inaaeaeoaaee oeaio?eooaoaaiiyi i?e 20 000 ia/oa. ia
i?ioyci 60 oa.

I?e ae?iuoaaiii ooai?a noaoieieiea a aaceeniaaiio na?aaeiaeui aiaa?iaii
oiiae noai?thaaeenue a aiaa?inoaoi i?e caiiii eiai aoiinoa?e ia
aaciiiaeiaiee acio.

Aeoeaiinoi eaoaaie?/ieo oa?iaioia aeieieico, iaioicioinoaoiiai oeyoo oa
oeeeeo o?eea?aiiiaeo eeneio aecia/aee a?aeiia?aeieie caaaeueiii?eeiyoeie
iaoiaeaie Aeoeai?noue aaenie?iace, eaeoaoaeaa?ae?iaaiace oa
ooia?aoaiae?aoace aecia/aee ca iaoiaeaie i?eaaaeaieie o Ei/aoiaa /1980/,
6-oinoio?oeoie?iace, aeueaeieace o?oeoici-1-6-aeeoinoaoo, i??oaaoe?iace,
IAAeI-aeaaiae?iaaiace, icioeeo?aoaeaaiae?iaaiace — ca iaoiaeaie
i?eaaaeaieie a Methods in enzymol./1982/,
aetheici-6-oinoaoaeaaiae?iaaiace oa 6-oinoiaetheiiaoaeaaiae?iaaiace ca
iaoiaeii Kornberg, Horecker /1955/, o?aineaoieace — ca iaoiaeii Dische a
iiaeeoieaoei? Brownstone, Denstedt /1961/, oeeo?aoneioace — ca iaoiaeii
Muller-Kraft, Babel /1983/, iaeaoaeaaiae?iaaiace — ca iaoiaeii E? E?i
Aaia /1970/.

Aeaii niaeo?ioioiiao?e/ieo aeii?thaaiue iaea?aeoaaee ia “Specord uv vis”
(I?ia//eia) i?e 300 N. Aeoeaiinoue oa?iaioia ae?aaeaee a iiieue
ia?aoai?aiiai noano?aoo ca 1 oaeeeio ia 1 ia aieea.

Iioaineaiinoue ieeneaiiy noano?aoia aeeoaeueiei eaioethaii aea/aeinue
iiey?ia?aoi/iei iaoiaeii ia iiey?ia?aoi LP-7 (*NN?), c aeei?enoaiiyi
cae?eoiai aeaeo?iaea oeia Eea?ea.

Aaia?aoe?th o?ainiaia?aiii? ??cieoe? aeaeo?e/ieo iioaioe?ae?a (( ia
oe?eeo ee?oeiao noao?eieiea aea/aee ca iaoiaeii, yeee ?ic?iaeaiee
Liberman, Skulachev/1970/ ?c canoinoaaiiyi neioaoe/ieo e?iio?eueieo
i?iieeath/eo ?ii?a oao?aoai?eoinoii?th (O??+). E??i oiai, aaia?aoe?th ((
aei??thaaee ii ?ioaineaiino? oeoi?anoeaioe??
8-ai?e?iiiaooae?i-1-noeueoiiaoo (AIN-).

Aecia/aiiy iaeiie/aiiy eeth/iaeo iaoaaieioia eaoaaieicio aetheice:
iiei/ii? eeneioe, ioeoiai? eeneioe, ii?iaeiia?aaeii? eeneioe i?iaiaeeee
ca iaoiaeaie i?eaaaeaieie o Anao?ai? /1961/. Eiioeaio?aoe?th NI2
aecia/aee iaiiiao?e/iei iaoiaeii a aia?ao? Aa?ao?aa. Aecia/aiiy
iaeiie/aiiy iaoaaieioia a aiaa?iaieo oiiaao i?iaiaeeee i?e iieoaoaaiii a
i?iai?eao Ooiaa?aa (aaeooi -4 ii ?o. noiaioey).

A aenia?eiaioao ii aei? iiaiaioi?a NaF ia iioaineaiinoue aeieieico
aeacaiee iiaiaioi? aiineee a iieoaaoeieia na?aaeiaeua a eiioeaio?aoei?
10-3 I.

Aecia/aiiy aeoeaiinoi a?ineioaoe/ieo oa?iaio?a ania?oao- oa
aeaiiiaiiiio?ainoa?ac i?iaiaeeee ca iaoiaeii Oaieaethi oa ?i. /1986/,
aoeaoee-NiA-ea?aieneeace – ca iaoiaeii Olsen and Merrik /1968/.

A aeine?aeao ii aeeth/aiith ii/aieo iiia?aaeieeia aieea oa ioeea?iiaeo
eeneio ee?oeie noao?eieie?a ae?iuoaaee aei ?aiiuei? aeniiiaioe?aeueii?
oace ia IIA i?e 370N. I?ney aecia/aiiy eiioeaio?aoe?? a?eea a ee?oeiao a
aeine?aei? i?ia??ee aiineee noniaic?th, ui i?noeea 1-2 ia a?eea.

Aeey oa?aeoa?enoeee neioaco a?eea aiineee: DL- [4- 14N ] ania?aa?iiao
eeneioo, DL –[iaoee-14 N] — iao?ii?i, [14 N] — na?ei, [14N] —
?cieaeoeei, [2,3-3I] -o?eioioai. Aeey aeine?aeaeaiiy neioaco ioeea?iiaeo
eeneio aiineee [8- 14N] — aaeai?i, [2-14N] — o?aoeee, [5-iao?e-3I] —
oei?i. Eiio?ieai neoaeeee noniaic?? ee?oei noao?eieiea, ui aoee
?iaeoeaiaai? i?ia??aaiiyi ia i?ioyc? 10 oa.

O?aeoe?iioaaiiy a?iiane i?iaiaeeee ca iaoiaeii Park, Hancock /1960/.
?aaeiiaeoeaiinoue aecia/aee ca aeiiiiiaith noeeioeeeyoeieiiai
ei/eeueieea oi?ie “ Intertechnigue” (O?aioeiy). ?ioaineai?noue
aeeth/aiiy i?/aieo iiia?aaeiee?a ioe?ithaaee a ?iioeuenao ca 1 oa ia 1
ia a?eea.

Aecia/aiiy aieea a aaceeioeiiiio aiiiaaiaoi i?iaiaeeee ca iaoiaeii Lowry
et al. /1951/. Noaoenoe/io ia?iaeo aenia?eiaioaeueieo aeaieo i?iaiaeeee
ii Eae?io /1990/

?acoeueoaoe aeine?aeaeaiue oa ?o iaaiai?aiiy

1.Aeoeai?noue eaoaaie?/ieo oa?iaio?a o ooai?a noao?eieiea, ui
aea/aeenue.

A ?iaio? aoei i?iaaaeaii ii??aiyeueia aea/aiiy aeoeaiino? eeth/iaeo
oa?iaio?a iniiaieo eaoaaie?/ieo oeyo?a oa iaeiie/aiiy oeaio?aeueieo
iaoaaie?o?a o /ooeeaiai oa ?acenoaioieo aei ?yaeo aioea?ioee?a ooai?a
noao?eieiea.

Ia ia?oiio aoai? aoei i?iaaaeaii aea/aiiy iaeiie/aiiy e?ioeaaeo
i?iaeoeo?a ae?eie?co. Anoaiiaeaii, ui a?eueoa /anoeia aetheice, yea
ooee?co?oueny ii noai? Aiaaeaia-Iaea?aioa -Ia?iana, ia?aoai?th?oueny a
iiei/io eeneioo. Oaeaee?noue iaeiie/aiiy iiei/ii? eeneioe ciaoiaeeoueny
a iaaeao 0,210-0,270 ieiieue ia 1 ia a?eea ca 1 aiae.
Aioea?ioeei?acenoaioi? ooaie noao?eieie?a oa?aeoa?ecothoueny a?eueoeie
ia 14-29% oaeaeeinoyie iaeiie/aiiy eaeoaoo. Ii?yae c oeei, a?aei?/aii
iaeiie/aiiy a iaaaeeeeo e?eueeinoyo aoeaoaoo oa NI2 (oaae.1).

Oaaeeoey 1

Iaeiie/aiiy e?ioeaaeo i?iaeoeoia aeieieico oeieeie eeioeiaie ooai?a
noaoieieiea a aiaa?iaieo oiiaao.

Ooaie E?euee?noue, ie iieue ia 1 ia a?eea ca 1 aiae.

noao?eieiea Eaeoao Aoeaoao NI2

8325-4 0,210+0,030 0,011+0,002 0,010+0,001

NO-4 0,270+0,040 0,014+0,003 0,012+0,002

NN-4 0,250+0,050 0,013+0,003 0,011+0,001

Ii??aiyeueia aea/aiiy iaeiie/aiiy eaeoaoo a aa?iaieo oa aiaa?iaieo
oiiaao o ooai?a noao?eieiea, ui aea/aeenue, aeaco? ia iacia/io ??cieoeth
a oaeaeeino? ae?eie?co a aeaieo oiiaao. Aoaeo Ianoa?a, ?ic?aoiaaiee ii
oi?ioe? A Eeaeioeaeea?a /1965/, aeinoeaaa 25,2-28,0% (oaae.2).

Oaaeeoey2

.Iaeiie/aiiy eaeoaoo oeieeie eeioeiaie ooai?a noaoieieiea a aa?iaieo oa
aiaa?iaieo oiiaao.

Ooaie E?euee?noue eaeoaoo, ieiieue ia 1ia a?eea ca 1 aiae Aoaeo

noao?eieiea aa?iai? oiiae aiaa?iai? oiiae Ianoa?a,

%

8325-4 0,157+0,021 0,210+0,030 25,2

NO-4 0,197+0,025 0,270+0,040 27,0

NN-4 0,180+0,035 0,250+0,050 28,0

Aeey c’ynoaaiiy ?aaoeyoi?ieo iaoai?ci?a ae?eie?co o noao?eieiea aoei
aea/aii iaeiie/aiiy eaeoaoo i?e aianaii? ?yaeo ?ioa?iaae?ao?a ia oii?
i?enooiino? a na?aaeiaeu? aetheice. Oaeaee?noue ae?eie?co c?inoa? i?e
aeiaeaaaii? aetheici-6-oinoaoo, o?oeoici-1,6-aeeoinoaoo oa i??oaaoo.
Iaie aoee c?iaeai? i?eiouaiiy, ui o noao?eieiea e?i?ooaaiiy ae?eie?co
iiaea a?aeaoaaoeny ia ??ai? aaenie?iace, oinoio?oeoie?iace,
i??oaaoe?iace. Aeieacii oeueiio iineoaeeee aecia/ai? iaie iecuee?
aeoeaiino? aeacaieo o?ueio oa?iaio?a a ii??aiyii? ?c aeueaeieacith
o?oeoici-1,6-aeeoinoaoo, yea ia a?aeiineoueny aei oa?iaio?a, ui
?aaoeththoue oaeaee?noue ae?eie?co.

A ?icaeoie io?eiaieo ?acoeueoao?a aoei i?iaaaeaii ioe?ieo ?ie? ae?eie?co
a caaaeueiiio eaoaaie?ci? aetheice ii aeoeaiino? aaenie?iace oa
eaeoaoaeaa?ae?iaaiace, a oaeiae ii ??aith iaeiie/aiiy i??iaeiia?aaeii?
eeneioe oa aieeao ia oeae i?ioean ?ia?a?oi?a ooi?enoiai iao??th o
aeine?aeaeo?ieo ooai?a noao?eieiea.

Aeoeai?noue aaenie?iace oa eaeoaoaeaa?ae?iaaiace o ooai?a, ye? ianooue
ieaci?aee ?acenoaioiino? aeua, i?ae o aeo?aeiiai ooaio ia 15-69%.
??aaiue aeoeaiino? a aiaa?iaieo oiiaao aeuee i?ae a aa?iaieo aeey an?o
ooai?a (oaae.3).

Aioea?ioeei?acenoaioi? ooaie oa?aeoa?ecothoueny ? a?eueoeie aaee/eiaie
iaeiie/aiiy i??oaaoo ia 29-57% (?en.1). NaF ?ia?ao? iaeiie/aiiy i??oaaoo
noao?eieieaie a aa?iaieo oiiaao ia 11-62%, a aiaa?iaieo oiiaao ia
35-61%. I?ioeaio ?ia?aoaaiiy aeey ?acenoaioieo aa??aio?a cia/ii aeuee a
ii??aiyii? ?c /ooeeaei ooaiii, ui na?ae/eoue i?i ia?aaaao ae?eie?oe/iiai
oeyoo a eaoaaie?ci? aoaeaaiae?a o ?acenoaioieo ooai?a.

Aeniea iioaineaiinoue aa?iaiiai aeieieico o noaoieieieia, ui oaeiae
aeyaeaia a ?yaei aeineiaeaeaiue (Aa?oaiiae/ /1962,1964/), iiaeeeai,
iiynith?oueny oei, ui noaoieieiee — oaeoeueoaoeaii aiaa?iaii
iie?ii?aaiicie.

Oaaeeoey 3

Aeoeaiinoue oa?iaio?a ae?eie?co o ooaiia noao?eieiea, /ooeeaiai oa

noieeeo aei aioeaiioeeia.(iiieue ca1oa. ia 1ia aieea).

Ooaie

noaoieieiea Aaenie?iaca Eaeoaoaeaa?ae?iaaiaca

Aa?iaii oiiae Aiaa?iaii oiiae Aa?iaii oiiae Aiaa?iai? oiiae

8325-4 15,0(1,2 16,8(0,4 ?(0,05 30,2(3,1 40,4(2,5 ?(0,05

NO — 1 13,1(0,3 ?(0,05 12,9(0,8 ?(0,05 48,2(5,2 ?(0,05 51,3±4,5
?(0,05

NO — 4 17,9(0,6 ?(0,05 19,6(0,7 ?(0,05 39,4±3,4 ?(0,05 50,3(4,2 ?(0,05

NN — 1 17,3(0,9 ?(0,05 19,4(0,6 ?(0,05 34,7(2,3 ?(0,05 49,3(4,1 ?(0,05

NN — 4 15,8(0,4 ?(0,05 18,1(0,7 ?(0,05 43,2(1,6 ?(0,05 46,4(2,5 ?(0,05

994 18,1(0,8 ?(0,05 17,4(0,9 ?(0,05 47,1(3,2 ?(0,05 52,4(2,7 ?(0,05

NA — 1 17,1(0,4 ?(0,05 20,0(0,6 ?(0,05 24,6(3,7 ?(0,05 24,3(3,4 ?(0,05

NF — 8 18,9(0,4 ?(0,05 20,3(0,4 ?(0,05 51,1(4,4 ?(0,05 64,3(6,2 ?(0,05

NA — 5 17,6(0,7 ?(0,05 19,5(0,6 ?(0,05 42,8(3,4 ?(0,05 54,3(2,7 ?(0,05

NF — 2 18,0(0,6 ?(0,05 19,3(0,5 ?(0,05 39,6(4,8 ?(0,05 44,3(5,2 ?(0,05

Aeueoa?iaoeaiei ae?eie?co ? iaioicioinoaoiee oeyo, yeee iinoaaey?
?ioa?iaae?aoe aeey i?ioean?a a?ineioaco. I?e aiae?c? ?acoeueoao?a ii
aeoeaiino? oa?iaio?a iaioicioinoaoiiai oeeeeo iaie aoei anoaiiaeaii, ui
aeoeai?noue oa?iaio?a ieenethaaeueiiai aoaio aeaa?ae?iaaiac
aetheici-6-oinoaoo oa 6-oinoiaetheiiaoo cieaeaia o ?acenoaioieo aei
aioea?ioee?a aa??aio?a a na?aaeiueiio ia 10-38 % a ii??aiyii? ?c
/ooeeaei ooaiii (?en2).

Aeoeai?noue oa?iaioa a?ineioaoe/ii? /anoeie -o?aineaoieace oaeiae ieae/a
o no?eeeo aa??aio?a ia 14-48%. Aeey an?o ooai?a oa?aeoa?ii iacia/ia
ca?eueoaiiy aeoeaiino? oa?iaio?a iaioicioinoaoiiai oeeeeo a aa?iaieo
oiiaao.

Oaeei /eiii, aeey ?acenoaioieo aei ??cieo aioea?ioee?a aa??aio?a
oa?aeoa?ia ci?ia ni?aa?aeiioaiiy iniiaieo eaoaaie?/ieo i?ioean?a:
i?aeaeuaiiy ae?eie?oe/iiai oeyoo ia oii? cieaeaiiy ?ioaineaiino?
iaioicioinoaoiiai oeeeeo.

3.Iniaeeaino? i?ioean?a ieeneaiiy o no?eeeo aei aioea?ioee?a

noao?eieie?a

A ?acoeueoao? ii??aiyeueiiai aeine?aeaeaiiy /ooeeaiai oa ?acenoaioieo
aa??aio?a ii ieeneaiith ?yaeo noano?ao?a iaie anoaiiaeaii, ui
noao?eieiee aeoeaii ieeneththoue aetheico, i??oaao oa aoeaoao (oaae.4).
Niiaeeaaiiy eenith i?e ieeneaii? aetheice ciaoiaeeoueny a iaaeao
47,1-83,6 iiieue ca 1oa. ia 1ia a?eea. No?ee? aei aioea?ioee?a aa??aioe
oa?aeoa?ecothoueny a?eueoeie ia 25-77% aaee/eiaie ieeneaiiy aetheice.
Aea aaea i?e ieeneaii? i??oaaoo ??cieoey a oaeaeeino? ieeneaiiy i?ae
aeo?aeiei ooaiii oa ?acenoaioieie aa??aioaie cia/ii ciaioo?oueny. A
ii??aiyeueiee aiae?c oaeaeeino? ieeneaiiy aoeaoaoo aeaco? ia cieaeaiiy
aeoeaiino? ieenethaaeueieo oa?iaio?a OeOE o ?acenoaioieo aa??aio?a oa
a?eueo ?ioaineaia oieinoa ieeneaiiy.

Oaaeeoey 4

Ieenethaaeueia aeoeai?noue oe?eeo ee?oei ooai?a
noao?eieiea

Ooaie Oaeaee?noue iiaeeiaiiy eenith iiieue I2 oa-1. ia-1 a?eea

noao?eieiea Aetheica I??oaao Aoeaoao

8325-4 47,1 ( 3,0 31,1 ( 3,4 15,2 ( 2,7

NT-1 75,8 (6,3 ?(0,05 25,3 ( 4,3 ?(0,05 12,4 ( 2,5 ?(0,05

NC-1 83,6 ( 7,1 ?(0,05 27,5 ( 5,6 ?(0,05 12,5 ( 3,5 ?(0,05

994 71,5 ( 6,8 ?(0,05 21,8 ( 3,7 ?(0,05 11,1 ( 2,8 ?(0,05

NF-8 59,0 ( 4,0 ?(0,05 20,8 ( 3,1 ?(0,05 9,9 ( 1,9 ?(0,05

NF-2 69,3 ( 5,7 ?(0,05 20,3 ( 4,6 ?(0,05 9,8 ( 1,5 ?(0,05

?acoeueoaoe aeine?ae?a ii ieeneaiith ?yaeo ai?iieeneio ooaiaie
noao?eieiea iieacaee iaio ?ioaineaia ieeneaiiy a ii??aiyii? c
aetheicith.

Oaaeeoey 5

?ioaineai?noue ieeneaiiy ?yaeo ai?iieeneio oe?eeie ee?oeiaie ooai?a
noao?eieiea.

Ooaie Oaeaee?noue ieeneaiiy, iiieue I2 oa-1. ia a?eea-1

noao?eieiea Ania?aa?iiaa eeneioa Iao?ii?i ?cieaeoeei O?eioioai Na?ei

8325-4 14,0( 3,6 12,3(3,5 11,4(3,0 10,5(3,1 15,3(3,5

NT-1 11,8( 2,7 10,9(2,2 10,7(3,2 9,6(2,0 12,5(2,9

NC-1 11,5( 2,4 11,0(2,9 10,9(2,8 9,5(2,1 12,0(1,8

994 11,0( 2,0 10,5(1,7 10,0(3,1 9,3(1,9 11,9(2,4

NF-8 10,2 (2,8 10,4(2,1 9,0(2,0 9,0(1,5 10,1(2,5

NF-2 10,4( 2,2 10,1(1,8 9,5(1,9 9,2(2,3 10,2(2,3

Aaee/eie ieeneaiiy a na?aaeiueiio neeaee 9-15 iiieue I2 ca oa. ia ia
a?eea (oaae.5). Aeacoth/e ia iaaaeeeo ??cieoeth i?ae ?acenoaioieie
aa??aioaie oa /ooeeaei ooaiii, iiaeia a?aei?oeoe oeaio?aeueio
oaiaeaioe?th aei cieaeaiiy ?ioaineaiino? ieeneaiiy ai?iieeneio o no?eeeo
ooai?a, ui i?aeoaa?aeaeo? ?ai?oa c?iaeaiee aeniiaie, i?i caaaeueia
cieaeaiiy ieenethaaeueieo oa?iaio?a o aeaieo ooai?a.

C iaoith c’ynoaaiiy, ye a?aeaoaa?oueny ieeneaiiy ai?iieeneio o ooai?a
noao?eieiea /a?ac aeeoaeueiee eaioetha, /e oea iia,ycaii c oieinoei
ieeneaiiyi, iaie i?iaaaeaii aecia/aiiy aaia?aoe?? o?ainiaia?aiii?
??cieoe? aeaeo?e/ieo iioaioe?ae?a ia nii?yaath/?e iaia?ai? noao?eieie?a
i?e aeei?enoaii? a yeino? noano?aoa — ai?iieeneio. Iaea?aeai?
?acoeueoaoe na?ae/aoue i?i iinooiaiiy a?aeiiaethth/eo aea?aaeaio?a a
aeeoaeueiee eaioetha i?e ieeneaii? ai?iieeneio.

I?i cieaeaiiy ieenethaaeueii? aeoeaiino? o ?acenoaioieo ooai?a
noao?eieie?a iiaeia noaa?aeaeoaaoe ? ia iniia? ciaioaiiy aeoeaiino?
eeth/iaiai oa?iaioo OeOE -oeeo?aoneioace ia 20-37% a ii??aiyii? c
/ooeeaei aa??aioii. Aea, a oie aea /an, i?e aecia/aii? aeoeaiino?
oa?iaio?a, iia’ycaieo c i?ioeanaie a?ineioaco — cai?ioiuei?
iaeaoaeaa?ae?iaaiace oa ooia?aoa?ae?aoace ie aeyaeee ca?eueoaiiy
aeoeaiino? oa?iaio?a o no?eeeo ooai?a a na?aaeiueiio ia 15-86% (?en3).

A aiaa?iaieo oiiaao aeoeai?noue oa?iaio?a OeOE i?iyaey?oueny, aea
cieaeaia a na?aaeiueiio ia 10-37%.

I?eaaaeai? aeai? na?ae/aoue i?i cieaeaiiy ieenethaaeueii? aeoeaiino?, o
?acenoaioieo aa??aio?a oa i?i iai?aaeai?noue ?ioa?iaae?ao?a OeOE ia
a?ineioaoe/i? i?ioeane.

4.A?ineioaoe/i? i?ioeane noao?eieie?a

A?aeiii, ui aia?aaoe/iee iai?i o?nii aca?iiiia,ycaiee c a?ineioaoe/ieie
?aaeoe?yie. Iaie aoee aecia/ai? aeoeaiino? oa?iaio?a neioaco
ai?iieeneio- ania?oao- oa aeai?iai?iio?ainoa?ac o eieaeoe?? ooai?a
noao?eieiea (oaae.6). Anoaiiaeaii, ui ?aaeoe?y ia?aai?ioaaiiy

Oaaeeoey 6

Aeoeaiinoue ania?oao- oa aeai?iaiiiio?ainoa?ac o ooaiia noaoieieiea,

yei noieei aei aioeaiioeeia (iiieue noano?aoo ca 1oa. ia 1 ia aieea
)

Ooaie noaoieieiea Ania?oaoai?iio?ainoa?aca Aeai?iai?iio?ainoa?aca

Aa?iaii oiiae Aiaa?iaii oiiae Aa?iaii oiiae Aiaa?iaii oiiae

8325-4 23,4(2,4 29,5( 1,3 ?(0,05 10,6(0,7 11,9(0,3 ?(0,05

NO — 1 30,4(1,6 ?(0,05 34,4( 0,9 ?(0,05 12,5(0,4 ?(0,05 14,0( 0,7 ?(0,05

NO — 4 36,0(2,1 ?(0,05 41,9(0,7 ?(0,05 16,0(1,2 ?(0,05 17,0 ( 0,9 ?(0,05

NN — 1 28,0(0,6 ?(0,05 31,8, (1,3 ?(0,05 15,3(0,2 ?(0,05 14,6 ( 0,4
?(0,05

NN — 4 38,4(0,7 ?(0,05 39,3(1,8 ?(0,05 12,7(0,6 ?(0,05 14,9 ( 0,5 ?(0,05

994 39,8(2,3 ?(0,05 41,9 (0,3 ?(0,05 14,8(0,9 ?(0,05 16,7 ( 0,3 ?(0,05

NA — 1 36,5(0,5 ?(0,05 37,4(1,1 ?(0,05 18,0(0,7 ?(0,05 19,5 ( 0,4 ?(0,05

NF — 8 25,7(1,3 ?(0,05 30,4(1,6 ?(0,05 12,1(0,9 ?(0,05 12,7 (1,1 ?(0,05

NA — 5 34,4(0,4 ?(0,05 36,0(1,2 ?(0,05 14,4(0,2 ?(0,05 15,4 ( 0,6
?(0,05

NF — 2 30,4(2,0 ?(0,05 34,2(0,8 ?(0,05 17,8(0,5 ?(0,05 19,1 ( 0,3
?(0,05

ai?iieeneio a?aeaoaa?oueny ?ioaineai?oa o no?eeeo ooai?a ia 20-70%. A
aiaa?iaieo oiiaao aeoeai?noue oa?iaio?a aeua a ii??aiyii? ?c aa?iaieie
aeey an?o ooai?a, aeeth/ath/e aeo?aeiee, ui iiaeeeai iia,ycaii ?c
cieaeaiiyi ieenethaaeueieo i?ioean?a oa iineeaiiyi ?aaeoe?e
a?ineioaco.Aiaeia?/i? ?acoeueoaoe aoee iaea?aeai? ? ii aeeth/aiith
ai?iieeneio a ee?oeie noao?eieie?a (oaae.7). ?acenoaioi? aa??aioe
oa?aeoa?ecothoueny a?eueoei ia 14-31% aaee/eiaie aeeth/aiiy
ania?aa?iiai? eeneioe, iao?ii?io, ?cieaeoeeio, o?eioioaio oa na?eio. Oea
iiaea na?ae/eoe i?i iineeaiiy neioaco a?eea o ooai?a noao?eieie?a, ye?
?acenoaioi? aei aioea?ioee?a.

Oaaeeoey 7

Aeeth/aiiy ii/aieo aiiiieeneio a ee?oeie /ooeeaiai oa
noieeeo ooaiia noaoieieiea (?ii.ca oa-1.ia ia a?eea-1)

Ooaie noaoieieiea Ania?aaiii-aa eeneioa O?eioi-oai Na?ei Iaoiiiii
?cieaeoeei

8325-4 8340(317 5910(400 7120(560 6810(340 7380(506

NT-1 7839(405 ?(0,05 6737(210 ?(0,05 6194(310 ?(0,05 7491(420 ?(0,05
9077(114 ?(0,05

NC-1 9924(470 ?<0,05 6974(220 ?<0,05 7761(150 ?(0,05 7763(210 ?(0,05 7675(300 ?(0,05 994 10008(320 ?(0,05 7565(417 ?(0,05 8473(480 ?(0,05 8512(610 ?(0,05 8708(280 ?(0,05 NF-8 9674(280 ?(0,05 7683(250 ?(0,05 8829(610 ?(0,05 8921(706 ?(0,05 8856(410 ?(0,05 NF-2 10675(510 ?(0,05 6914(320 ?(0,05 8402(340 ?(0,05 8035(510 ?(0,05 8339(530 ?(0,05 Aeey aea/aiiy ?ioaineaiino? neioaco ioeea?iiaeo eeneio aoei i?iaaaeaii aeeth/aiiy acioenoeo iniia a ee?oeie noao?eieie?a. ?acoeueoaoe iieacaee a?eueo ?ioaineaia aeeth/aiiy ?acenoaioieie aa??aioaie. Aaeai?i oa oei?i aeeth/athoueny ia 12-45% aeoeai?oa, o?aoeee ia 10-20% (?en.5) A?eueoa aeoeai?noue oa?aeoa?ia ? aeey neioaco e?i?ae?a o ?acenoaioieo aa??aio?a. Ia oea aeaco? ca?eueoaiiy ia 32-89% aeoeaiino? aoeaoee-EiA-ea?aieneeace-eeth/iaiai oa?iaioo o neioac? e?i?ae?a o no?eeeo ooai?a a ii??aiyii? c /ooeeaei ooaiii.(?en.4). Iaea?aeai? ?acoeueoaoe ii aeoeaiino? a?ineioaoe/ieo oa?iaio?a, aeeth/aiith ai?iieeneio oa acioenoeo iniia aeacothoue ia a?eueo ?ioaineaiee neioac iaea?eueo aaaeeeaeo eean?a niieoe- ioeea?iiaeo eeneio, a?ee?a oa e?i?ae?a o no?eeeo aei aioea?ioee?a aa??aio?a. AENIIAEE 1. O noao?eieie?a, ye? acyo? aeey aeine?ae?a, iniiaia /anoeia aetheice, ui ooee?co?oueny ii noai? Aiaaeaia-Iaea?aioa-Ia?iana ia?aoai?th?oueny a iiei/io eeneioo, iaeiie/aiiy eaeoaoo neeaaea? 0,210-0,270 ieiieue, e??i oiai a i?i?iaeueieo e?eueeinoyo iaeiie/o?oueny aoeaoao oa NI2. Aoaeo Ianoa?a aeinoeaaa 25,2-28,0%. E?i?ooaaiiy ae?eie?co cae?enith?oueny ia ??ai? aaenie?iace, oinoio?oeoie?iace, i??oaaoe?iace. 2.Aioea?ioeei?acenoaioi? aa??aioe noao?eieie?a oa?aeoa?ecothoueny a?eueo aenieeie ia 15-26% aeoeaiinoyie aaenie?iace oa ia 15-69% eaeoaoaeaa?ae?iaaiace ye a aa?iaieo oae ? a aiaa?iaieo oiiaao. 3.Aeoeai?noue aeaa?ae?iaaiac aetheici-6-oinoaoo oa 6-oinoiaetheiiaoo ia 10-38%, o?aineaoieace ia14-48% ieae/a o ?acenoaioieo ooai?a noao?eieiea, ui na?ae/eoue i?i cieaeaiiy aeoeaiino? iaioicioinoaoiiai oeeeeo. 4.Ieeneaiiy aetheice oe?eeie ee?oeiaie noao?eieiea aeua ia 25-77% o ?acenoaioieo aa??aio?a, a i??oaao oa aoeaoao ia 15-25% ?ioaineai?oa ieeneththoueny /ooeeaei ooaiii. O no?eeeo aei aioea?ioee?a ooai?a ieae/a aeoeai?noue iniiaieo aia?aiiinoa/ath/eo ?aaeoe?e OeOE oa a?eueo ?ioaineaia oieinoa ieeneaiiy. 5.Aeoeai?noue aoeaoee-EiA-ea?aieneeace ia 32-89%, iaeaoaeaa?ae?iaaiace ia 15-36%, ooia?aoa?ae?aoace ia 15-86%, aeua o ?acenoaioieo aa??aio?a, ui na?ae/eoue i?i iai?aaeaii?noue iioieo ?ioa?iaae?ao?a OeOE ia a?ineioaoe/i? i?ioeane. 6.Ooaie noao?eieiea, ye? no?ee? aei aioea?ioee?a, oa?aeoa?ecothoueny a?eueo aenieeie ia (20-70%) aeoeaiinoyie oa?iaio?a ai?iieeneioiiai iai?io - ania?oao- oa aeai?iai?iio?ainoa?ac. 7.?acenoaioi? aei aioea?ioee?a aa??aioe ia 14-31% ?ioaineai?oa aeeth/athoue i?/ai? ai?iieeneioe: ania?aa?iiao eeneioo, iao?ii?i, o?eioioai, ?cieaeoeei, na?ei a ii??aiyii? ?c /ooeeaei aa??aioii, ui aeaco? ia a?eueo ?ioaineaiee neioac a?eea. 8.?aae?iaeoeai? acioeno? iniiae –aaeai?i, oei?i oa o?aoeee - ia 10-45% ?ioaineai?oa aeeth/athoueny ee?oeiaie ?acenoaioieo ooai?a, i?ae /ooeeaei ooaiii, ui iiaea na?ae/eoe i?i a?eueo ?ioaineaiee neioac ioeea?iiaeo eeneio. Nienie ioae?eaoe?e ii oai? aeena?oaoe?? 1. Aaa?eethe A.A.,Eiceoeeay N.I.,Aieiaeie E.I.,Aeiieeia A.E. Aeoeaiinoue eeth/aauo oa?iaioia aeeeieeoe/aneiai e iaioicioinoaoiiai iooae o ieacieaeiniaea?aeaueo noaoeeieieeia// Oe?. aeioei. aeo?i.-1996.-?1.-N.45-48. 2. Aaa?eethe A.A.,Eiceoeeay N.I.,Aieiaeie E.I.,Aeiieeia A.E. Iniaaiiinoe iaoaaieecia noaoeeieieeia,onoie/eauo e aioeaeioeeai// Aanoiee AeAO na?ey Aeieiaey e aeieiaey .-1996.-N.31-38. 3. Eiceoeeay N.I.,Aaa?eethe A.A., Aieiaeie E.I.,Aeiieeia A.E.Aeoeaiinoue ?yaea oa?iaioia aiaaiee/aneiai iaiaia o noaoeeieieeia, niaea?aeaueo ieacieaeu onoie/eainoe e aioeaeioeeai// Iee?iaeie.aeo?i.-1997.-?1.- N.37-41. 4. N.I.Eiceoeeay A.A.Aaa?eethe E.I.Aieiaeie A.E.Aeiieeia Iniaaiiinoe i?ioeannia eaoaaieecia oaeaaiaeia o ieacieaeiniaea?aeaueo noaoeeieieeia // Nai?iee AeAO“?aaoeyoeey a aeeauo nenoaiao”.-1998.- N.67-71 5. Eiceoeueea N.I. ?ioaineai?noue aeeth/aiiy i?/aieo ai?iieeneio a oiae? a?ineioaco a?eea o noao?eieie?a, ye? no?ee? aei aioea?ioee?a// Oac.VII Oe?.a?io?i. c ?caeo.- Ee?a.-N.138-139. 6. N.I.Eiceoeueea A.A.Aaa?eethe E.I.Aieiaeie A.?.A?ii?eia Aeoeai?noue ?yaeo oa?iaio?a aiaaie?/iiai iai?io o noao?eieie?a, ye? i?noyoue ieaci?aee no?eeino? aei aioea?ioee?a// Oac.VII Oe?.a?io?i. c ?caeo.- Ee?a.-N.139-140. 7..Kozitskaia S.N.,.Gavrilyuck V.G.,Golodok L.P.,Vinnikov A.I.Regulation du metabolisme constructif des staphylocoques contenant des plasmides de resistance aux antibiotiques// V-e Conference Internat.France Ukraine T.3.- 1998.-P.66-67 8. Eiceoeueea N.I.,Aaa?eethe A.A.,Aieiaeie E.I.,A?ii?eia A.?. Aea/aiiy iniiaieo iaoaaie?/ieo ?aaeoe?e o no?eeeo aei aioea?ioee?a noao?eieie?a//1 I?aeia?iaeia iaoeiai-i?aeo. eiio. “Iaoea oa ina?oa”.-Aei?i?iiao?ianuee.- 1998.-N.62-63. Eiceoeueea N.I. Aca?iica’ycie aia?aaoe/iiai oa eiino?oeoeaiiai iai?io o ooai?a noao?eieiea, ye? no?ee? aei aioea?ioee?a.-?oeiien. Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo iaoe ca niaoe?aeuei?noth 03-00-07-i?e?ia?ieia?y.-?inoeooo i?e?ia?ieia?? ? a??onieia?? IAI Oe?a?ie, Ee?a, 1999. Aeena?oaoe?y i?enay/aia aea/aiith ?aaeoe?e aia?aaoe/iiai oa eiino?oeoeaiiai iai?i?a o /ooeeaiai oa ?acenoaioieo aei ?yaeo aioea?ioee?a ooai?a noao?eieiea. ?acoeueoaoe aeine?aeaeaiue iieacaee, ui no?ee? aei aioea?ioee?a ooaie noao?eieiea oa?aeoa?ecothoueny ?ioaineo?eaoe??th ?aaeoe?e ae?eie?oe/iiai ?icuaieaiiy aoaeaaiae?a. Iniiaia /anoeia aetheice ia?aoai?th?oueny a iiei/io eeneioo, a iacia/ieo e?eueeinoyo iaeiie/o?oueny aoeaoao oa NI2. Aoaeo Ianoa?a aeinoeaaa 25,2-28,0%. Iieacaii, ui no?ee? aa??aioe noao?eieiea oa?aeoa?ecothoueny a?eueoeie ia 15-69% aeoeaiinoyie aaenie?iace oa eaeoaoaeaa?ae?iaaiace. Aeoeai?noue oa?iaio?a iaioicioinoaoiiai oeeeeo – aeaa?ae?iaaiac aetheici-6-oinoaoo oa 6-oinoiaetheiiaoo, a oaeiae o?aineaoieace cieaeaia o ?acenoaioieo aa??aio?a ia (10-38% oa 14-48% a?aeiia?aeii). Iieacaii, ui aetheico aeoeai?oa ieeneththoue ?acenoaioi? aa??aioe, a aoeaoao oa i??oaao- /ooeeaee ooai. Aeoeai?noue eeth/iaiai oa?iaioa OeOE-oeeo?aoneioace o no?eeeo ooai?a cieaeaia ia oii? ?ioaineo?eaoe?? ooia?aoa?ae?aoace. Anoaiiaeaii, ui aeoeai?noue a?ineioaoe/ieo oa?iaio?a –o?ainai?iac aeai?io oa ania?oaoo, a oaeiae aeoeai?noue aoeaoee-EiA-ea?aieneeace aeua o ?acenoaioieo aa??aio?a a na?aaeiueiio ia 20-89%. I?e aiae?c? aeeth/aiiy i?/aieo iiia?aaeiee?a a?eea oa ioeea?iiaeo eeneio a ee?oeie noao?eieie?a iieacaii a?eueo ?ioaineaia aeeth/aiiy ?acenoaioieie aa??aioaie a ii??aiyii? ?c aeo?aeiei /ooeeaei ooaiii. Eeth/ia? neiaa: noao?eieiee, ieaci?aee, aioea?ioeei?acenoaioi?noue, aia?aaoe/iee iai?i, ?aaeoe?? a?ineioaco. Eiceoeeay N.I. Acaeiinaycue yia?aaoe/aneiai e eiino?oeoeaiiai iaiaia o ooaiiia noaoeeieieea, onoie/eauo e aioeaeioeeai.-?oeiienue Aeena?oaoeey ia nieneaiea iao/iie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.07-iee?iaeieiaey.-Einoeooo iee?iaeieiaee e ae?onieiaee IAI Oe?aeiu, Eeaa, 1999. Aeena?oaoeey iinayuaia eco/aieth ?aaeoeee aia?aaoe/aneiai e eiino?oeoeaiiai iaiaiia o /oanoaeoaeueiiai e ?acenoaioiuo e ?yaeo aioeaeioeeia ooaiiia noaoeeieieea. ?acoeueoaou enneaaeiaaiee iieacaee, /oi onoie/eaua e aioeaeioeeai ooaiiu noaoeeieieea oa?aeoa?ecothony eioaineoeeaoeeae ?aaeoeee aeeeieeoe/aneiai ?anuaieaiey oaeaaiaeia. Iniiaiay /anoue aetheicu i?aa?auaaony a iiei/ioth eeneioo, a iacia/eoaeueiuo eiee/anoaao iaeaieeaaaony aoeaoao e NI2. Aaee/eiu iaeiieaiey eaeoaoa aeey enneaaeoaiuo ooaiiia a aa?iaiuo oneiaeyo ninoaaeee 0,157 – 0,197 ieiieue ia 1 ia aaeea ca 1 /an, a aiaa?iaiuo –0,210 – 0,270 ieiieue. Aioeaeioeei?acenoaioiua ooaiiu noaoeeieieeia oa?aeoa?ecothony aieueoeie ia 14-29% aaee/eiaie iaeiieaiey eaeoaoa a ay?iaiuo e aiay?iaiuo oneiaeyo a n?aaiaiee n /oanoaeoaeueiui ooaiiii. Yooaeo Ianoa?a aeinoeaae 25,2—28,0%. I?e eco/aiee nei?inoe aeeeieeca ia aaneeaoi/iii aiiiaaiaoa i?e aianaiee ?yaea eioa?iaaeeaoia auei onoaiiaeaii, /oi nei?inoue aeeeieeca oaaee/eaaaony i?e aeiaaaeaiee aetheici-6-oinoaoa, o?oeoici-1,6-aeeoinoaoa, ie?oaaoa, a oiaea a?aiy eae o?oeoici-6-oinoao, aeeoea?aeueaeaaeae-3-oinoao, 3-oinoiaeeoea?ao, 2-oinoiaeeoea?ao, oinoiaiieie?oaao iiaeiaiiai aeaenoaey ia ieacuaatho. Iieacaii, /oi onoie/eaua aa?eaiou noaoeeieieea oa?aeoa?ecothony aieueoeie ia 15-69% aeoeaiinoyie aaenieeiacu e eaeoaoaeaaeae?iaaiacu. Iaeiieaiea ie?oaaoa eeaoeaie ?acenoaioiuo ooaiiia oaeaea aieueoa ia 29-57% a n?aaiaiee n /oanoaeoaeueiui ooaiiii. NaF a eiioeaio?aoeee 10-3 I eiaeae?oao iaeiieaiea ie?oaaoa enneaaeoaiuie ooaiiaie. I?ioeaio eiaeae?iaaiey aeey enoiaeiiai ooaiia ieaea eae a ay?iaiuo oae e a aiay?iaiuo oneiaeyo, /ai aeey ?acenoaioiuo ooaiiia. Aeoeaiinoue oa?iaioia iaioicioinoaoiiai oeeeea-aeaaeae?iaaiac aetheici-6-oinoaoa e 6-oinoiaetheiiaoa, a oaeaea o?aineaoieacu nieaeaia o ?acenoaioiuo aa?eaioia ia (10-38% e 14-48% niioaaonoaaiii). A ?acoeueoaoa n?aaieoaeueiiai enneaaeiaaiey /oanoaeoaeueiiai e ?acenoaioiuo aa?eaioia ii ieeneaieth ?yaea noano?aoia onoaiiaeaii, /oi eeaoee ?acenoaioiuo ooaiiia noaoeeieieea aeoeaii ieeneytho aetheico, a ie?oaao e aoeaoao - /oanoaeoaeueiue ooaii. Ieeneaiea aieiieeneio enneaaeoaiuie ooaiiaie i?ienoiaeeo iaiaa eioaineaii, /ai aetheicu e ioia/aii iacia/eoaeueiia nieaeaiea ieeneaiey o ?acenoaioiuo aa?eaioia. Aeoeaiinoue eeth/aaiai oa?iaioa OeOE-oeeo?aoneioacu o onoie/eauo ooaiiia nieaeaia ia oiia eioaineoeeaoeee ooia?aoaeae?aoacu. Ii?aaeaeaii, /oi aeoeaiinoue aeineioaoe/aneeo oa?iaioia -o?ainaieiac aeaieia e ania?oaoa auoa o ?acenoaioiuo aa?eaioia a n?aaeiai ia 20-70%, Aiaeiae/iua ?acoeueoaou auee iieo/aiu e i?e enneaaeiaaiee aeeth/aiey aieiieeneio a eeaoee noaoeeieieeia. ?acenoaioiua aa?eaiou oa?aeoa?ecothony aieueoei ia 14-31% o?iaiai aeeth/aiey ania?aaeiiaie eeneiou, iaoeiieia, ecieaeoeeia, o?eioioaia e na?eia. Aeoeaiinoue oa?iaioa, naycaiiiai n neioacii eeieaeia - aoeaoee-EiA-ea?aieneeacu oaeaea auoa ia 32-89% o ooaiiia, onoie/eauo e aioeaeioeeai. Aeeth/aiea ia/aiuo i?aaeoanoaaiieeia ioeeaeiiauo eeneio a eeaoee noaoeeieieeia aieaa eioaineaii inouanoaeyaony ?acenoaioiuie aa?eaioaie a n?aaiaiee n enoiaeiui /oanoaeoaeueiui ooaiiii. Eeth/aaua neiaa: noaoeeieieee, ieacieaeu, aioeaeioeei?acenoaioiinoue, yia?aaoe/aneee iaiai, ?aaeoeee aeineioaca. S.N.Kozitskaya. Interrelation of Energetic and Constructive Metabolism in staphylococcus strains resistant to antibiotics. -Manuscript. The dissertation on competition of a scientific degree of the candidate of biological sciences on a speciality 03.00.07- microbiology. –Institute of microbiology and virology Nati.Acad.Sci.of Ukraine, Kyiv, 1999. The dissertation is devoted to the investigation of the energetic and constructive metabolism reactions in staphylococcus strains sensitive and resistant to some antibiotics. The investigation results have shown that staphylococcus strains resistant to antibiotics are characterized by the intensification of Carbohydrates glycolitic splitting reactions. Glucose major part transforms into dairy acid. Acetate and CO2 are accumulated in small amounts. Paster effect reached 25,2 — 28,0%. It is shown that staphylococci resistant variants are characterized by 15-69 % larger hexokinase and lactate dehydrogenase activities. Ensyme activities of pentosophosphate cyclo dehydrogenase glucose-6-phosphate and 6-phosphogluconate and transketolase are reduced in resistant variants on (10-38 % and 14-48 % respectively) .It is shown that glucose is oxidized more actively by resistant variants, and acetate and pyruvate are oxidized by sensitive strains. Citrate syntase key enzyme activity in resistant strains is increased on the fumarase intensification background. It is determined that the activity of biosynthetic enzymes, alanin transaminase and aspartat transaminase and also acetyl-KoA-carboxylase activity is higher in resistant variants on the average on 20-89%. The involrement of proteins and nucleic acids labelled predecessors into staphylococci cells more intensive in resistant variants compared to the initial sensitive strain. Key words: staphylococci, plasmids, antibioticresistance, energetic metabolism, biosynthesis reactions. . iao/iie 1-8325-4, 2 - NO-1, 3 - NO-4, 4 - NN-1, 5 - NN-4, 6 - 994, 7 - NF-8. Iaoe?iiaeueia aeaaeai?y iaoe oe?a?ie ?inoeooo i?e?ia?ieia?? oa a??onieia?? ?I. Ae.E.caaieioiiai Eiceoeueea Na?oeaia Ieeiea?aia oaee 579.222.6 Aca?iica’ycie aia?aaoe/iiai oa eiino?oeoeaiiai iai?io o ooai?a noao?eieiea, ye? no?ee? aei aioea?ioee?a 03-00-07-i?e?ia?ieia?y Aaoi?aoa?ao aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo iaoe Ee?a – 1999 Aeena?oaoe??th ? ?oeiien. ?iaioa aeeiiaia ia eaoaae?? i?e?ia?ieia?? oa a??onieia?? Aei?i?iiao?ianueeiai aea?aeaaiiai oi?aa?neoaoo. Iaoeiaee ea??aiee: aeieoi? a?ieia?/ieo iaoe, i?ioani? A?ii?eia Aeueaa?o ?aaiiae/ Io?oe?ei? iiiiaioe: aeieoi? a?ieia?/ieo iaoe Nieieia ?aai Aai?a?eiae/ aeieoi? iaaee/ieo iaoe Naeueieeiaa Ieueaa Iao??aia I?ia?aeia i?aai?caoe?y - Ee?anueeee Iaoe?iiaeueiee oi?aa?neoao ?i.Oa?ana Oaa/aiea Caoeno a?aeaoaeaoueny 17 ethoiai 1999?ieo i 10 aiaeei? ia can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae 26.233.01.i?e ?inoeooo? i?e?ia?ieia?? oa a??onieia?? ?i Ae.E.Caaieioiiai IAI Oe?a?ie (252143, Ee?a, aoe.Caaieioiiai, 154, ?inoeooo i?e?ia?ieia?? ? a??onieia?? IAI Oe?a?ie, cae can?aeaiue) C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? ?inoeoooo i?e?ia?ieia?? ? a??onieia?? IAI Oe?a?ie. Aaoi?aoa?ao ?ic?neaiee “______”____________________ 1998?. A/aiee nae?aoa? niaoe?ae?ciaaii? a/aii? ?aaee eaiaeeaeao a?ieia?/ieo iaoe Io??o E.I. PAGE 21 - PAGE 21 PAGE 21 PAGE 2

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