OE?A?INUeEA AEAAeAI?ss AA?A?IEO IAOE

?INOEOOO CAIEA?IANOAA ? A?IEIA?? OAA?EI

IIEIOEEI IEAA NOAIAIIAE*

OAeE 636.2:
612.015.3: 591.13: 591.175

AIEEA O?C?IEIA?*IIAI NOAIO ? OAEOI??A AEEAEAIIss IA IAI?I ?A*IAEI A
I?AAI?CI? EI??A

03.00.04 – a?io?i?y

A a o i ? a o a ? a o

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe

Euea?a – 1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia a ?inoeooo? caiea?ianoaa ? a?ieia?? oaa?ei OAAI

( i. Euea?a)

Iaoeiaee ea??aiee: aeieoi? a?ieia?/ieo iaoe, i?ioani?

ssIIAE* Aaaeei Aai?a?eiae/,

?inoeooo caiea?ianoaa ? a?ieia?? oaa?ei
OAAI,

caa?aeoaa/ eaai?aoi???th a?ineioaco
i?iaeoeo?a

oaa?eiieoeoaa.

Io?oe?ei? iiiiaioe: aeieoi? a?ieia?/ieo iaoe, i?ioani?

Aiae Noao Eineiiae/,

Euea?anueeee aea?aeaaiee
aa?a?iee oi?aa?neoao,

caa?aeoaa/ eaoaae?ith
oaa?eiieoeoaa;

aeieoi? a?ieia?/ieo iaoe

Ae?iiee A?eai??e Aaneeueiae/,

Euea?anueea aea?aeaaia aeaaeai?y

aaoa?eia?ii? iaaeeoeeie ?i. N. C.
Aaeeoeueeiai,

caa?aeoaa/ eaoaae?ith oaoiieia?? iieiea ?
iiei/ieo

i?iaeoeo?a.

I?ia?aeia onoaiiaa: Oa?e?anueeee ciiaaoa?eia?iee ?inoeooo

I?i?noa?noaa aa?ii?iieneiaiai
eiiieaeno Oe?a?ie,

eaoaae?a a?io?i??.

Caoeno a?aeaoaeaoueny “ 22 ” /a?aiy 1999 ?. i “ 9-00 ” aiaeei? ia

can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae 35.368.01. a ?inoeooo?
caiea?ianoaa ? a?ieia?? oaa?ei OAAI ca aae?anith 290034, i. Euea?a,
aoe. A.Noona, 38.

C aeena?oaoe??th iiaeia iiciaeiieoeny o a?ae?ioaoe? ?inoeoooo
caiea?ianoaa ? a?ieia?? oaa?ei OAAI ca aae?anith 290034, i. Euea?a, aoe.
A.Noona, 38.

Aaoi?aoa?ao ?ic?neaiee “ 20 ” o?aaiy 1999 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee
*a?e?i A.A.

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue oaie. Anaa?/ia aea/aiiy iai?io ?a/iaei a i?aai?ci? ei??a
a caeaaeiino? a?ae o?c?ieia?/iiai noaio, oaeoi??a aiae?ae? ? oo?eiaiiy ?
iaiao?aeiith ia?aaeoiiaith ni?yiiaaiiai aieeao ia ?o i?iaeoeoeai?noue,
ye?noue iieiea, iieaoo ei?io. Cie?aia, iaoeiaee ? i?aeoe/iee ?ioa?an
noaiiaeoue c’ynoaaiiy o?c?ieiai-a?io?i?/ieo iaoai?ci?a, c yeeie
iia’ycai? iniaeeaino? iai?io ?a/iaei a i?aai?ci? ei??a i?ioyaii
o?eueiino? ? eaeoaoe?? ? oaeoi??a ?o ?aaoeyoe??. Aeooaeuei?noue oaeeo
aeine?aeaeaiue iaoiiaeaia iaaeinoaoi?i c’ynoaaiiyi a?io?i?/ieo
iaoai?ci?a, ye? eaaeaoue a iniia? cieaeaiiy aeeai? iane
aenieii?iaeoeoeaieo ei??a o ii/aoeiaee ia??iae eaeoaoe?? [Iiaiaeaaa
N.O., 1991; Gibb M.J. et.al.,1992; McNamara J.P., 1994; Waltner S.S. et.
al., 1994] ci?i iaoaaie?/iiai i?io?eth a ?o e?ia? i?ioyaii aaa?oiino? ?
eaeoaoe?? [Ia/a?aiei Y. A. e ae?., 1987; Halse K., Tveit B., 1994;
Gueorquier I.P. et. al., 1995].

Iaoeiaee ? i?aeoe/iee ?ioa?an noaiiaeoue oaeiae aeine?aeaeaiiy aieeao
oaeoi??a aeeaeaiiy ia iiei/io i?iaeoeoeai?noue ei??a ? iaoaaie?/iee
i?io?eue a ?o e?ia?. Aeooaeuei?noue aea/aiiy oeueiai ieoaiiy iaoiiaeaia
iaiao?aei?noth aeaeueoiai aaeineiiaeaiiy ?nioth/i? a Oe?a?i? nenoaie
aeeaeaiiy aenieii?iaeoeoeaieo ei??a, yea ia a?aoiaa? ?yae i?eioeei?a ?
iieiaeaiue, ye? iieeaaeai? a iniiao a nenoaiao aeeaeaiiy ei??a ?yaeo
e?a?i [ARC, 1984; NRC, 1985, 1989]. Oea noino?oueny, cie?aia,
iaiao?aeiino? c’ynoaaiiy aieeao ??ciiai ni?aa?aeiioaiiy i?ae ai?noii
?icuaiethaaiiai ? ia?icuaiethaaiiai a ?oaoe? i?ioa?io, a oaeiae ??ciiai
ni?aa?aeiioaiiy i?ae ai?noii oeoe?o ? e?ioiaeth a ?aoe?ii? ei??a ia
iiei/io i?iaeoeoeai?noue, ia neioac aaeoa??aeueiiai i?ioa?io ?
i?iaeoeoe?th iecueeiiieaeoey?ieo aee?ieo eeneio o ?oaoe?, ia nooi?iue
iiaeeiaiiy ai?iieeneio, aetheice ? aee?ieo eeneio iiei/iith caeicith ?
aeei?enoaiiy ?o o neioac? eiiiiiaio?a iieiea. Iayai? a e?oa?aoo?? noaoo?
c oeueiai ieoaiiy [Naaanoueyiiaa I.A, 1980; Oethiei A.A., 1981;
Ia/a?aiei Y.A., 1986, 1991; ?aae/aieia A.I., 1989; Iaothuaiei I.A.,
1992; Baldwin R.L., 1994] iaaeinoaoiuei aena?oeththoue ?yae eiai
aniaeo?a.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. Aeena?oaoe?eia
?iaioa ? o?aaiaioii oaie Ae? 0196UI10484, oeo? 01.01.: “Aeine?aeeoe
iaoai?cie noano?aoii? ?aaoeyoe?? i?ioean?a o?aaeaiiy ? canai?iiy
iiaeeaieo ?a/iaei ei?io aeey neioaco neeaaeiaeo /anoei oeaiei i?aai?cio
? iieiea o aenieii?iaeoeoeaieo ei??a”, ui aeeiioaaeanue a eaai?aoi???
aeeaeaiiy ei??a ? ?aaoeyoe?? iieieiooai?aiiy ?inoeoooo caiea?ianoaa ?
a?ieia?? oaa?ei OAAI.

Iaoa ? caaaeaiiy aeine?aeaeaiiy. Iaoith aeena?oaoe?eii? ?iaioe aoei
aeine?aeeoe aieea o?c?ieia?/iiai noaio ? oaeoi??a aeeaeaiiy ia
iaoaaie?/iee i?io?eue a e?ia? ? a?io?i?/i? i?ioeane o neaeaoieo i’ycao
ei??a oa ?o i?iaeoeoeai?noue.

Aeey aeinyaiaiiy oe??? iaoe aoee iinoaaeaii? ianooii? caaaeaiiy:

– aeine?aeeoe iaoaaie?/iee i?io?eue o e?ia ? ei??a a ia??iae
o?eueiino?, ia ii/aoeo ? a e?ioe? eaeoaoe?? oa i?e ??cieo oeiao
aiae?ae?;

– aecia/eoe ?ioaineai?noue neioaco ? ?iciaaeo a?ee?a, neioaco
e?i?ae?a ? nooi?iue aeei?enoaiiy aee?ieo eeneio, aetheice ? ai?iieeneio
a aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao ei??a a oiiaao in vitro a
ia??iae o?eueiino? ? ia aeacaieo noaae?yo eaeoaoe??;

– c’ynoaaoe aieea ?aoe?ii?a c ??ciei ni?aa?aeiioaiiyi
?icuaiethaaiiai ? ia?icuaiethaaiiai i?ioa?io, a oaeiae c ??ciei
ni?aa?aeiioaiiyi i?ae ai?noii e?ioiaeth ? oeoe?o o ?aoe?ii? ei??a ia
canai?iiy iiaeeaieo ?a/iaei ei?io, ia ??aaiue ai?iieeneio, aetheice ?
aee?ieo eeneio a ieaci? e?ia? ? ?o iiaeeiaiiy iiei/iith caeicith, ia
i?iaeoeoeai?noue ei??a ? neeaae iieiea.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aia?oa i?iaaaeaii ii??aiyeueia
aeine?aeaeaiiy ?ioaineaiino? neioaco a?ee?a ? ?o ?iciaaeo, ?ioaineaiino?
neioaco e?i?ae?a c ??cieo iiia?aaeiee?a ? nooiaiy aeei?enoaiiy i?/aieo
?aae?iaeoeaiei aoaeaoeai aee?ieo eeneio, aetheice ? ai?iieeneio a
aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao ei??a o ia??iae o?eueiino?, ia
ii/aoeo ? a e?ioe? eaeoaoe??. A ?acoeueoao? oeeo aeine?aeaeaiue
anoaiiaeaii caeaaei?noue neioaco e?i?ae?a, neioaco ? ?iciaaeo a?ee?a o
neaeaoieo i’ycao ei??a a?ae ?o o?c?ieia?/iiai noaio, a?eueoee aieea
o?eueiino? ? noaae?? eaeoaoe?? ei??a ia neioac a?ee?a o neaeaoieo
i’ycao, i?ae ia ?o ?iciaae. Anoaiiaeaia e?euee?nia noi?iia aeei?enoaiiy
aetheice, aee?ieo eeneio ? ai?iieeneio o noano?aoiiio caaacia/aii?
neioaco ie?aieo eean?a e?i?ae?a ? aia?aaoe/ieo i?ioean?a o neaeaoieo
i’ycao ei??a ca ??ciiai o?c?ieia?/iiai noaio. Iaea?aeaii iia? aeai?
i?i aieea oeio aiae?ae? ia iaoaaie?/iee i?io?eue o e?ia? ei??a, i?i
aieea ??a aiae?ae? ia canai?iiy iiaeeaieo ?a/iaei ei?io o o?aaiiio
eaiae? ei??a, ?o iiaeeiaiiy iiei/iith caeicith ? aeei?enoaiiy o neioac?
eiiiiiaio?a iieiea aiinyoue cia/iee aeeaae a ?ic?iaeo
o?c?ieiai-a?io?i?/ieo iniia aeeaeaiiy ei??a ? iiaeooue aooe aeei?enoai?
i?e noai?aii? iiai? nenoaie aeeaeaiiy ei??a. Anoaiiaeaiee aieea
o?c?ieia?/iiai noaio, oeio aiae?ae? ? neeaaeo ?aoe?iio ia iaoaaie?/iee
i?io?eue e?ia? iiaea iineoaeeoe iniiaith aeey ?ic?iaee iaoiae?a
eiio?ieth e?i?ooth/eo i?ioeane iieieiooai?aiiy eaiie iai?io ?a/iaei.

aiae?ae? ia canai?iiy iiaeeaieo ?a/iaei ei?io o o?aaiiio eaiae? ei??a,
?o iiaeeiaiiy iiei/iith caeicith ? aeei?enoaiiy o neioac? eiiiiiaio?a
iieiea aiinyoue cia/iee aeeaae a ?ic?iaeo o?c?ieiai-a?io?i?/ieo iniia
aeeaeaiiy ei??a ? iiaeooue aooe aeei?enoai? i?e noai?aii? iiai? nenoaie
aeeaeaiiy ei??a. Anoaiiaeaiee aieea o?c?ieia?/iiai noaio, oeio aiae?ae?
? neeaaeo ?aoe?iio ia iaoaaie?/iee i?io?eue e?ia? iiaea iineoaeeoe
iniiaith aeey ?ic?iaee iaoiae?a eiio?ieth e?i?ooth/eo i?ioeane
iieieiooai?aiiy eaiie iai?io ?a/iaei.

Iniaenoee aianie caeiaoaa/a. I?aea?? ? ii?aoethaaiiy a?o/eciyii? ?
ca?oa?aeii? e?oa?aoo?e ii oai? aeena?oaoe?eii? ?iaioe aeeiiai? aaoi?ii
iniaenoi. Aeena?oaio naiino?eii aecia/aa a?io?i?/i? iieacieee e?ia?
ei??a, ?ioaineai?noue neioaco ? ?iciaaeo a?ee?a, neioaco e?i?ae?a ?
aeei?enoaiiy aetheice, aee?ieo eeneio ? ai?iieeneio a aia?aaoe/ieo
i?ioeanao o neaeaoieo i’ycao ei??a. Aeine?aeaeaiiy aieeao oaeoi??a
aeeaeaiiy ia iai?i ?a/iaei a i?aai?ci? ei??a i?iaaaeaii ?acii ?c
ni?a?ia?oieeaie eaai?aoi??? aeeaeaiiy aeoeieo ? ?aaoeyoe??
iieieiooai?aiiy ?inoeoooo caiea?ianoaa ? a?ieia?? oaa?ei OAAI, ye? ?
ni?aaaoi?aie aeayeeo iaoeiaeo i?aoeue aeena?oaioa. Aiae?c ? iaaiai?aiiy
io?eiaieo aeaieo i?iaaaeaii ni?eueii c iaoeiaei ea??aieeii ?
caa?aeoaa/ai eaai?aoi???.

Ai?iaaoe?y ?acoeueoao?a aeine?aeaeaiue. Iaoa??aee aeena?oaoe?eii? ?iaioe
aeiiia?aeaeenue ia: ca?oi?e nan?? ?inoeoooo caiea?ianoaa ? a?ieia??
oaa?ei OAAI (1997); eiioa?aioe?yo iieiaeeo a/aieo aeacaiiai ?inoeoooo
(1997,1998); i?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe?? “No/ani?
i?iaeaie a?ieia??, aaoa?eia?ii? iaaeeoeeie, cii?iaeaia??? oa oaoiieia?e
i?iaeoeo?a oaa?eiieoeoaa (Euea?a, 1997); i?aeia?iaei?e
iaoeiai-i?aeoe/i?e eiioa?aioe?? iieiaeeo a/aieo “Iaoeia? aeinyaiaiiy a
aaeoc? aaoa?eia?ii? iaaeeoeeie” (Oa?e?a, 1997); VII Oe?a?inueeiio
a?io?i?/iiio c’?cae? (Ee?a, 1997), XVII conf. “Dni fyziologie
hospodarskych zvierat”(Kosice, 1997); XV c’?cae? Oe?a?inueeiai
o?c?ieia?/iiai oiaa?enoaa (Aeiiaoeuee-Euea?a, 1998); ???
i?aeaeoia?iaeiiio neiiic?oi? “A?ieia?/i? iaoai?cie noa??iiy (Oa?e?a,
1998); II i?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe?? “I?iaeaie
ia?ioaeoe?eii? iaoieia?? oaa?ei” (A?ea Oea?eaa, 1998); i?aeia?iaei?e
eiioa?aioe?? “A?ieia?/i? iniiae aeeaeaiiy n?euenueeiainiiaea?nueeeo
oaa?ei” (Euea?a, 1998); i?aeeaai?aoi?iiio can?aeaii? ?inoeoooo
caiea?ianoaa ? a?ieia?? oaa?ei OAAI (1999).

Ioae?eaoe?? ?acoeueoao?a aeine?aeaeaiue. Ca ?acoeueoaoaie aeine?aeaeaiue
iioae?eiaaii 10 iaoeiaeo ?ia?o, a yeeo aeeeaaeaii iniiaiee ci?no
iaoeiaeo aeine?aeaeaiue.

No?oeoo?a oa ia’?i aeena?oaoe??. Aeena?oaoe?eia ?iaioa aeeeaaeaia ia 171
noi??ieao iaoeiiieniiai oaenoo, i?noeoue 29 oaaeeoeue, neeaaea?oueny c
anooio, iaeyaeo e?oa?aoo?e, iaoa??ae?a ? iaoiae?a aeine?aeaeaiue,
aeanieo aeine?aeaeaiue, iaaiai?aiiy ?acoeueoao?a aeine?aeaeaiue,
aeniiae?a, nieneo aeei?enoaieo aeaea?ae, yeee i?noeoue 408 aeaea?ae,
na?aae yeeo 306 ?iicaii?.

AEA?? IAI?ssIE?A AeINE?AeAEAIUe, IAOA??AE OA IAOIAeE AEEIIAIIss ?IAIOE

C iaoith aeine?aeaeaiiy aieeao o?c?ieia?/iiai noaio ? oaeoi??a aiae?ae?
ia iaoaaie?/iee i?io?eue a e?ia? ? a?io?i?/i? i?ioeane o neaeaoieo
i’ycao ei??a i?iaaaeaii aea? na??? aeine?ae?a. Aenia?eiaioaeueia /anoeia
ia?oi? na??? aeine?ae?a ?iaioe aeeiiaia ia 57 aieiaao aaeeei? ?iaaoi?
ooaeiae (54-o ei?iaao ? 3-o oaeeoeyo) a naeyinuee?e ni?eoe?
“Caa??’yinueea” Cai??anueeiai ?aeiio Oa?iii?euenueei? iaeano?.

O ia?o?e na??? aeine?ae?a i?iaaaeaii aeine?aeaeaiiy ?yaeo a?io?i?/ieo
ii-eaciee?a o e?ia? ei??a a e?ioe? ia?oiai ? nueiiiai i?nyoey eaeoaoe??,
a oaeiae o nooino?eieo ei??a o e?ioe? aeaa’oiai i?nyoey o?eueiino?. O
e?ia? ei??a ia aeacaieo noaae?yo eaeoaoe?? ? a e?ioe? o?eueiino?
aecia/aee ai?no aetheice [Aaeaoianeee E.N., 1987], iaaoa?eo?eiaaieo
aee?ieo eeneio [I?ioi?iaa I.E., 1982], a?eueieo ai?iieeneio [Muting D.,
1963], na/iaeie [Coulombe J.J., 1963], caaaeueiee a?eie ne?iaaoee e?ia?
?ao?aeoiiao?e/ii [Aieaei A.E., 1974] ? ni?aa?aeiioaiiy ie?aieo eiai
o?aeoe?e iaoiaeii aeaeo?ioi?aco ia aaa?? [Eeeia A., Ieeieia O., 1959],
caaaeueiee ai?no e?i?ae?a ? ni?aa?aeiioaiiy ie?aieo ?o eean?a
iaoiaeii oiieioa?iai? o?iiaoia?ao?? ia nee?eaaae? [Eaeon M., 1975],
a oaeiae aee?iieeneioiee neeaae caaaeueieo e?i?ae?a iaoiaeii
aaci??aeeiii? o?iiaoia?ao?? [Eaeon M., 1975]. Aeacai? aeine?aeaeaiiy
i?iaaaeaii o ianiaeuiee ? no?eeiaee ia??iaee (o aeiaoi?, a?oaei?,
ethoiio ? ea?oi?), ui aeicaieeei c’ynoaaoe oaeiae aieea oei?a aiae?ae? ?
neeaaeo ?aoe?iio ia aeine?aeaeoaai? iieacieee. A aeiaoi? i?nyoe? ei?iae
niiaeeaaee ceaeiai-aiaia? o?aae ? caeaio iano eoeo?oaece, o a?oaei? ?
ethoiio io?eioaaee ?aoe?ii neeiniiai, a ea?oi? — neeiniiai ?
neeinii-n?iaaeiiai oei?a. Ii?ioaaiiy aiae?ae? i?iaiaeeee ca
aeaoae?ciaaieie ii?iaie [Eaeaoieeia A.I. e ae?., 1985].

Aeey aeine?aeaeaiiy aieeao o?c?ieia?/iiai noaio ia neioaoe/i? ?
aia?aaoe/i? i?ioeane o neaeaoieo i’ycao aeei?enoiaoaaee c?acee
/ioe?ueioaieiaiai i’yca noaaia ei??a ? oaeeoeue, iaea?aeai? oeyoii
a?iin?? [Aeeaa A.A., 1974].

Aeine?aeaeaiiy ?ioaineaiino? neioaco a?ee?a o neaeaoiiio i’yc?
i?iaiaeeee oeyoii ?ieoaaoe?? aiiiaaiao?a i’yca o oinoaoiiio aooa??
E?aan-??iaa?a, aei yeiai aeiaeaaaee 1 ieEth??
D,L-3-oai?e-[1-14C]-aeai?io c ianooiiei aecia/aiiyi ?aae?iaeoeaiino?
neioaciaaieo a?ee?a ia ??aeeiiiio noeeioeeyoe?eiiio e?/eeueieeo LKB
(Oaaoe?y) [I?ioi?iaa I.E., 1982] o oieoieiaiio noeeioeeyoi?? i?ney
aeno?aeoe?? c aiiiaaiaoo e?i?ae?a. Aeine?aeaeaiiy ?ioaineaiino? neioaco
e?i?ae?a o neaeaoiiio i’yc? i?iaiaeeee oeyoii ?ieoaaoe?? aiiiaaiao?a
i’yca a ?ieoaaoe?eiiio na?aaeiaeu? o oinoaoiiio aooa?? E?aan-??iaa?a,
aei yeiai aeiaeaaaee 1ieEth?? [1-14C] ioeoiai?, [1-14C] noaa?eiiai?
eeneio, [6-14C] aetheice aai D,L-3-oai?e-[1-14C]-aeai?io c ianooiiei
aecia/aiiyi ?aae?iaeoeaiino? ooai?aiiai NI2 ? neioaciaaieo e?i?ae?a ia
aeacaiiio e?/eeueieeo. I?e oeueiio aecia/aee ?aae?iaeoeai?noue ie?aieo
eean?a e?i?ae?a, ye? i?ney aeno?aeoe?? caaaeueieo e?i?ae?a noi?ooth
oei?ioi?i-iaoaiie 2:1 [Folch G. et.al., 1957] aeae?eyee oeyoii
oiieioa?iai? o?iiaoia?ao?? ia nee?eaaae? o nenoai? aaioai-aeeaoeeiaee
ao??-eueiaeiaa ioeoiaa eeneioa o a?aeiioaii? 70:30:1 [Eaeon M., 1975].

Aeoeai?noue eeneeo (pH-3.0) ? iaeo?aeueieo (pH-7.0) i?ioaac o neaeaoieo
i’ycao ei??a ? oaeeoeue aecia/aee oeyoii ?ieoaaoe?? aiiiaaiao?a,
aeai-oiaeaieo c aeine?aeaeoaaiiai i’yca a?aeiia?aeii a aoeaoaoiiio ?
oinoaoiiio aooa?ao, ye? i?noeee ye noano?ao i?ioaie?co a?aeiia?aeii
aaiiaeia?i ? eaca?i, c ianooiiei aecia/aiiyi o na?aaeiaeu? e?eueeino?
oe?iceio [Aaeayi A.I., Eaaeoeueeee A.I., 1982]. Ai?no ?ic/eiieo a?ee?a o
?ieoaaoe?eiiio na?aaeiaeu? aecia/aee ca iaoiaeii Eio?? ? ni?aaaoi??a
[Lowry I.I. et. al., 1951].

O ae?oa?e na??? aeine?ae?a, aeeiiaieo o aeine?aeiiio ainiiaea?noa?
?inoeoooo caiea?ianoaa ? a?ieia?? oaa?ei OAAI “*eoee” aea/aany aieea
?aoe?ii?a c ??ciei ni?aa?aeiioaiiyi, c iaeiiai aieo, i?ae ai?noii oeoe?o
? e?ioiaeth, a c ae?oaiai — i?ae ai?noii eaaei- ? aaaeei?icuaiethaaiiai
i?ioa?io ia canai?iiy iiaeeaieo ?a/iaei ei?io ? aeei?enoaiiy
aia?aaoe/ieo ? ieanoe/ieo noano?ao?a o neioac? eiiiiiaio?a iieiea.
Aeine?aee i?iaaaeai? iaoiaeii ia??iae?a ia o?ueio ei?iaao /i?ii-?yai?
ii?iaee, 5-6 ??/iiai a?eo, c iaaei?i ca eaeoaoe?th 3800-4200 e iieiea.

O ia?oiio (eiio?ieueiiio) ia??iae? o?eaae?noth 30 aei?a aiae?aey ei??a
cae?enithaaeanue ca ?aoe?iiii, caaeainiaaiei ca iniiaieie aeaiaioaie
aeeaeaiiy ca?aeii aeaoae?ciaaieo ii?i [Eaeaoieeia A.I. e ae?., 1985].
E?euee?noue ?icuaiethaaiiai i?ioa?io a ?aoe?ii? ei??a noaiiaeea 71%,
a?aeiioaiiy oeoe?o aei e?ioiaeth — 1:1,6. O ae?oaiio ia??iae? (30
aei?a) a?aeiioaiiy oeoe?o aei e?ioiaeth noaiiaeei 1:1,1. Ci?ia
ni?aa?aeiioaiiy aeinyaaeanue oeyoii aaaaeaiiy a ?aoe?ii aeno?oaeiaaiii?
y/i?iii? ? ioaie/ii? aea?o? ? i?aeai?ii ei?i?a. Ioeoe?thaaiiy e?ioiaeth
oeyoii aeno?oaeoaaiiy eiioeaio?ao?a i?eaiaeeei aei ca?eueoaiiy a
?aoe?ii? /anoee iiii- ? aeenaoa?eae?a ia 13,4%. O o?aoueiio ia??iae? (30
aei?a) a ?aoe?ii? ei??a e?euee?noue aaaeei?icuaiethaaiiai i?ioa?io aoea
ia 11.5% a?eueoa, i?ae o eiio?ieueiiio ia??iae?. Ca?eueoaiiy /anoee
aaaeei?icuaiethaaiiai i?ioa?io a ?aoe?ii? ei??a aeinyaaeanue oeyoii
aaaaeaiiy a iueiai ia?iaeaii? oi?iaeueaeaa?aeii niiyoieeiai? iaeooe.

O e?ioe? eiaeiiai ia??iaeo i?iaiaeeee aaeainia? aeine?aee c iaoith
aea/aiiy ia?ao?aaiino? ? canai?iiy iiaeeaieo ?a/iaei ei?io: nooi?
?a/iaeie, i?ioa?io ee?oeiaeie, ne?iai aee?o, AA?. Aeey a?io?i?/ieo
aeine?aeaeaiue aeei?enoiaoaaee e?ia c /a?aaii? ai?oe ? iiei/ii? aaie,
eae, na/o ? iieiei. A e?ia? aecia/aee ai?no ioeoiai? eeneioe
[Aieiaaoeueeee ?.Ae., 1961], eaoiiiaeo o?e [Iaoaeuenii, 1961],
iaaoa?eo?eiaaieo aee?ieo eeneio [I?ioi?iaa I.E., 1982], aetheice
aetheiciieneaeaciei iaoiaeii, caaaeueiee ai?no a?ee?a ?ao?aeoiiao?e/ii,
ai?no caeeoeiaiai acioo ca iaoiaeii E’?eueaeaey ? na/iaeie [Coulombe
J.J., 1963]. O iieioe? aecia/aee ai?no aee?o, caaaeueiiai ? caeeoeiaiai
acioo, acioo eaca?i?a, aeueaoi?i?a ? aeiaoe?i?a, eaeoice, na/iaeie
caaaeueiiaaeeaaieie iaoiaeaie.

Io?eiai? oeeo?ia? aeai? ii?aoeueiaoaaee noaoenoe/ii.

?ACOEUeOAOE AeINE?AeAEAIUe ? ?O IAAIAI?AIIss

Aieea o?c?ieia?/iiai noaio ? oeio aiae?ae? ia iaoaaie?/iee i?io?eue a
e?ia? ei??a. Ia oeueiio aoai? ?iaioe aoei i?iaaaeaii ii??aiyeueia
aeine?aeaeaiiy ai?noo a?ee?a, e?i?ae?a ? ?o iaoaaie?o?a o e?ia? ei??a o
e?ioe? ia?oiai, nueiiiai i?nyoe?a eaeoaoe?? ? a e?ioe? aeaa’yoiai
i?nyoey o?eueiino? i?e caiaeiaoaaii? ?i caeaieo ei?i?a, ?aoe?ii?a
neeiniiai ? neeinii-n?iaaeiiai oeio. I?ioyaii eaeoaoe?? ia
niinoa??aa?oueny aeinoia??ieo ci?i caaaeueiiai a?eeo o ne?iaaooe? e?ia?
ei??a, oi/ ??aaiue eiai aeaui aeuee ia ii/aoeo eaeoaoe??, ui iaoiiaeaii
ca?eueoaiiyi o oeae ia??iae eiioeaio?aoe?? aeueaoi?i?a (?<0,05). Eiioeaio?aoe?y g-aeiaoe?i?a aoea aeuith a e?ioe? eaeoaoe?? ? o?eueiino? (?<0,05). Ai?no ai?iiiai acioo a ne?iaaooe? e?ia? ei??a ia ii/aoeo eaeoaoe?? a?eueoee, i?ae a e?ioe? eaeoaoe?? ? o?eueiino?, ui iiaeia iiynieoe aeyaeaiei iaie iineeaiiyi ?iciaaeo a?ee?a neaeaoieo i’yc?a. Eiioeaio?aoe?y na/iaeie o ieaci? e?ia? ei??a o e?ioe? eaeoaoe?? ? o?eueiino? aoea cia/ii a?eueoa i?e an?o oeiao aiae?ae? (?<0,05 - 0,001), i?ae ia ii/aoeo eaeoaoe??. Oe? aeai? na?ae/aoue i?i i?aeaeuaiiy ?aoaioe?? acioo a i?aai?ci? ei??a a ia??iae ?ioaineaii? eaeoaoe??, ui iaoiiaeaii iineeaiei aeei?enoaiiyi iiei/iith caeicith oee?eoethth/eo o ieaci? e?ia? a?eueieo ai?iieeneio o neioac? a?ee?a iieiea ? cieaeaiiyi ?ioaineaiino? neioaco a?ee?a o neaeaoieo i’ycao. Iacaeaaeii a?ae o?c?ieia?/iiai noaio eiioeaio?aoe?y na/iaeie a ne?iaaooe? e?ia? ei??a i?e caiaeiaoaaii? ?i n?iaaeo a?eueoa, i?ae i?e caiaeiaoaaii? neeino. Caaaeueiee ai?no e?i?ae?a o ieaci? e?ia? ei??a o e?ioe? ia?oiai i?nyoey eaeoaoe?? aoa a?eueoee, i?ae o e?ioe? nueiiiai i?nyoey ?, iniaeeai, a e?ioe? nooino?eiiai ia??iaeo i?e an?o oeiao aiae?ae? (?<0,01-0,05). Ai?no IAAEE o ieaci? e?ia? ei??a ia ii/aoeo eaeoaoe?? a?eueoee, i?ae o e?ioe? eaeoaoe?? ? nooino?eiiai ia??iaeo (?<0,05), a i?e caiaeiaoaaii? neeino - , i?ae i?e caiaeiaoaaii? n?iaaeo ? neeino (?<0,5). Oae? ci?ie iiaeia iiynieoe iineeaiiyi iia?e?caoe?? e?i?ae?a c aee?iaeo aeaii ei??a o aeacaiee ia??iae o ca’yceo c ?ioaineaiith eaeoaoe??th [Emery R.S., 1979, McNamara J.R., 1994]. Oaaeeoey 1. Ai?no iaoaaie?o?a e?ia? ei??a ca ??ciiai o?c?ieia?/iiai noaio i?e neeiniiio oei? aiae?ae? (M±m, n=10) Aeine?aeaeoaai? O?c?ieia?/iee noai eiiiiiaioe Ii/aoie eaeoaoe?? E?iaoeue eaeoaoe?? Nooino?eiee ia??iae A?eee,% 8,37±0,22 7,87 ± 0,19 7,87± 0,26 E?i?aee,% 409±9,5 361±8,7* 324±8,3* IAAEE, ieiieue 225±5,7 193±6,3* 183±7,9* Aetheica, ia% 53,3±1,4 50,1±1,5 59,7±2,1* Na/iaeia, ia% 34,8±1,3 42,2±1,7* 46,5±1,6* I?ei?oea. O oe?e ? ianooii?e oaaeeoeyo *- a??ia?aei?noue ??cieo o aeine?aeaeoaaieo cia/aiiyo iieaciee?a (?<0,05-0,001) o e?ia? ei??a o e?ioe? eaeoaoe?? ? a nooino?eiee ia??iae ii??aiyii c ii/aoeii eaeoaoe??. Eiioeaio?aoe?y aetheice o ieaci? e?ia? ei??a ia ii/aoeo eaeoaoe?? ? a e?ioe? o?eueiino? a?eueoa (?<0,05), i?ae a e?ioe? eaeoaoe??. Oea na?ae/eoue i?i aieea ia eiioeaio?aoe?th aetheice a ieaci? e?ia? ei??a o?c?ieia?/iiai noaio, ye oaeoi?a, a?ae yeiai caeaaeeoue nooi?iue aeei?enoaiiy oeueiai iiiinaoa?eaeo iiei/iith caeicith aeey neioaco eaeoice ? iiei/iiai aee?o [Halse K., 1994], a oaeiae - o?ainii?o ?? /a?ac ieaoeaioo a i?aai?ci ieiaea [Hay W.W., 1984, 1988]. Iaie aeyaeai? ??cieoe? o ni?aa?aeiioaii? ie?aieo o?aeoe?e ne?iaaoeiaeo a?ee?a o ne?iaaooe? e?ia? ei??a i?e caiaeiaoaaii? ?i ?aoe?ii?a neeiniiai ? neeinii-n?iaaeiiai oeio. ?acii c oei, i?iaaaeai? aeine?aeaeaiiy iieacaee, ui oei aiae?ae? cia/iith i??ith aieeaa? ia aee?iieeneioiee neeaae caaaeueieo e?i?ae?a ieacie e?ia? ei??a. Cie?aia, o caaaeueieo e?i?aeao ieacie e?ia? ei??a, yeei caiaeiaoaaee ?aoe?ii neeiniiai oeio, aeyaeaii iaioee ai?no iaeuei?oeiiai? ? iea?iiai? ? a?eueoee ai?no e?iieaai? ? e?iieaiiai? eeneio, i?ae o ei??a, yeei caiaeiaoaaee ?aoe?ii neeinii-n?iaaeiiai oeio (?<0,05-0,01). A oe?eiio ?acoeueoaoe iaea?aeai? i?e aeeiiaii? oeueiai aoaia ?iaioe na?ae/aoue i?i aieea oeio aiae?ae? ? neeaaeo ?aoe?iio ia iaoaaie?/iee i?io?eue o e?ia? ei??a, ui ne?ae a?aoiaoaaoe i?e aeine?aeaeaii? aieeao o?c?ieia?/iiai noaio ei??a ia ai?no iaoaaie?o?a o ?o e?ia?. Aieea o?c?ieia?/iiai noaio ei??a ia iai?i a?ee?a o neaeaoieo i’ycao a oiiaao in vitro. Iaoith oeueiai aoaio aeena?oaoe?eii? ?iaioe aoei ii??aiyeueia aeine?aeaeaiiy, c iaeiiai aieo, ?ioaineaiino? neioaco ? ?iciaaeo a?ee?a o neaeaoieo i’ycao ei??a a e?ioe? ia?oiai ? nueiiiai i?nyoe?a eaeoaoe??, o iaeaeooth/eo ei??a a e?ioe? aeaa'yoiai i?nyoey o?eueiino? ? o 18-i?ny/ieo oaeeoeue, c ae?oaiai - aeine?aeaeaiiy ?ioaineaiino? neioaco e?i?ae?a c ??cieo iiia?aaeiee?a, c o?aoueiai - aeine?aeaeaiiy nooiaiy aeei?enoaiiy aetheice, aee?ieo eeneio ? ai?iieeneio o aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao aeine?aeaeoaaieo ei??a ? oaeeoeue a oiiaao in vitro. A ?acoeueoao? i?iaaaeaieo aeine?aeaeaiue anoaiiaeaii, ui ?aae?iaeoeai?noue a?ee?a i?e ?ieoaaoe?? aiiiaaiao?a neaeaoiiai i’yca ei??a c D,L-3-oai?e-[1-14N]-aeai?iii o e?ioe? ia?oiai i?nyoey eaeoaoe?? cia/ii iaioa, i?ae o e?ioe? nueiiiai i?nyoey (?<0,05) ? o e?ioe? nooino?eiiai ia??iaeo (?<0,01). Oe? aeai? na?ae/aoue, ui aeyaeaia ?yaeii aaoi??a [Gibb M.J., 1992; Eiieia H.A., 1992; Waltner S.S., 1994] ciaioaiiy aeeai? iane ei??a ? ai?noo a?ee?a a neaeaoieo i’ycao a ia??iae ?ioaineaii? eaeoaoe?? a ia?o? aeaa i?nyoe? i?ney ioaeaiiy iaoiiaeaii ia o?eueee iineeaiiyi ?iciaaeo i’ycaaeo a?ee?a, a e cieaeaiiyi ?ioaineaiino? ?o neioaco. C iaea?aeaieo ?acoeueoao?a aeieeaa?, ui i?aeaeuaia iiaeeiaiiy ai?iieeneio iiei/iith caeicith ei??a aeey neioaco a?ee?a iieiea a ia??iae ?ioaineaii? eaeoaoe?? i?eaiaeeoue aei ciaioaiiy aeei?enoaiiy ai?iieeneio o neioac? a?ee?a neaeaoieo i’yc?a. Iineeaiiy ?ioaineaiino? neioaco a?ee?a o neaeaoieo i’ycao ei??a o e?ioe? eaeoaoe?? ? iniaeeai a ia??iae o?eueiino? iaoiiaeaia noeioethth/ei aieeaii ?inoe?io aiane?aeie i?aeaeuaiiy eiai ??aiy o e?ia? [Bikkerstaffe R., 1993]. C i?eaaaeaieo o oaaeeoe? 2 aeaieo aeaeii, ui ?aae?iaeoeai?noue a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca oaeeoeue i?e ?ieoaaoe?? c D,L-3-oai?e-[1-14N]-aeai?iii ia 70, 43 ? 31% a?eueoa (?<0,001) ii??aiyii aei ?aae?iaeoeaiino? a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoieo i’yc?a ei??a a e?ioe? ia?oiai ? nueiiiai i?nyoe?a eaeoaoe?? ? o nooino?eiee ia??iae. Oea iiynith?oueny aieeaii ia ?ioaineai?noue neioaco a?ee?a o neaeaoieo i’ycao aaeeei? ?iaaoi? ooaeiae, c iaeiiai aieo, o?c?ieia?/iiai noaio, a c ae?oaiai - a?eo oaa?ei. C ?ioeo aniaeo?a iaea?aeaieo iaie ?acoeueoao?a caneoaiao? ia oaaao cia/ia ?aae?iaeoeai?noue a?ee?a i?ney ?ieoaaoe?? aiiiaaiao?a neaeaoiiai i'yca ei??a ? oaeeoeue c [6-14N] aetheicith ? [1-14N] ioeoiaith eeneioith. Cie?aia, ?aae?iaeoeai?noue a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca oaeeoeue, Oaaeeoey 2. ?aae?iaeoeai?noue a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca ei??a ? oaeeoeue i?ney ?ieoaaoe?? c ??cieie iiia?aaeieeaie (M±m, b-?ici./100ia ne?. oe./oa, n=3). Iiia?aaeieee E i ? i a e Oaeeoe? a?ee?a 1-e i?n.eaeo. 7-e i?n.eaeo. nooino?ei? D,L-3-oai?e-[1-14N]-aeai?i 5520±198 6562±265* 7175±372* 9400±293* [6-14N] aetheica 493±19 643±22* 755±37* 534±14 [1-14N] ioeoiaa eeneioa 641±30 779±29* 631±25 963±37* ei??a ia ii/aoeo ? a e?ioe? eaeoaoe?? ? a e?ioe? o?eueiino? i?ney ?ieoaaoe?? c [6-14N] aetheicith noaiiaeoue a?aeiia?aeii 5,67; 8,98; 4,80 ? 10,54% ?aae?iaeoeaiino? a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca aeacaieo oaa?ei i?e ?ieoaaoe?? c D,L-3-oai?e-[1-14N]-aeai?iii. ?aae?iaeoeai?noue a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca oaeeoeue, ei??a ia ii/aoeo ? a e?ioe? eaeoaoe?? ? nooino?eiiai ia??iaeo i?ney ?ieoaaoe?? c [1-14N] ioeoiaith eeneioith noaiiaeoue a?aeiia?aeii 10,23; 11,65; 8,82 ? 8,80% ?aae?iaeoeaiino? a?ee?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca oaa?ei i?ney ?ieoaaoe?? c D,L-3-oai?e-[1-14N]-aeai?iii. Oe? aeai? na?ae/aoue i?i aeei?enoaiiy a neioac? aoaeaoeaaiai neaeaoa cai?iieo ai?iieeneio o neaeaoieo i’ycao ei??a ? oaeeoeue aoaeaoeaaiai eaioethaa aetheice ? ioeoiai? eeneioe, i?i aieea ia nooi?iue oeueiai aeei?enoaiiy o?c?ieia?/iiai noaio ? a?eo oaa?ei. C i?eaaaeaieo a oaaeeoe? 3 aeaieo aeaeii, ui ??cieoe? o aeoeaiino? eeneeo i?ioaac o neaeaoieo i’ycao ei??a ia ii/aoeo ? a e?ioe? eaeoaoe??, a oaeiae a e?ioe? nooino?eiiai ia??iaeo noaoenoe/ii iaa??ia?aei? (?<0,5). Ia a?aei?io a?ae eeneeo i?ioaac, aeoeai?noue iaeo?aeueieo i?ioaac o neaeaoieo i’ycao ei??a a e?ioe? ia?oiai i?nyoey eaeoaoe?? cia/ii aeua, i?ae a e?ioe? nueiiiai (?<0,05) ? iniaeeai a e?ioe? aeaa’yoiai i?nyoey o?eueiino? (?<0,01). Oe? aeai? na?ae/aoue i?i a?eueo ?ioaineaiee ?iciaae i’yciaeo a?ee?a o ei??a ia ii/aoeo eaeoaoe??, yeee oa?aeoa?eco?oueny aenieeie iio?aaaie a ai?iieeneioao aeey neioaco iieiea. Oaaeeoey 3. Aeoeai?noue i?ioaac o neaeaoieo i’ycao ei??a ? oaeeoeue (M±m, ieiieue oe?iceio/a a?eeo, n=3). I?ioaace E i ? i a e Oaeeoe? 1-e i?n.eaeo. 7-e i?n.eaeo. Nooino?ei? Eene? 21,23±0,78 22,00±1,15 23,90±1,55 44,20±3,78* Iaeo?aeuei? 19,23±0,78 16,03±0,95* 14,00±0,81* 32,77±2,23* ?acii c oeei, iaea?aeai? ?acoeueoaoe na?ae/aoue i?i cia/ii aeuo aeoeai?noue eeneeo ? iaeo?aeueieo i?ioaac o neaeaoieo i’ycao oaeeoeue, i?ae o neaeaoieo i’ycao ei??a, ui iaoiiaeaii ?ioaineaiei iiiaeaiiyi i’yciaeo a?ee?a [Lobley, 1980]. Aieea o?c?ieia?/iiai noaio ia neioac e?i?ae?a o neaeaoieo i’ycao ei??a a oiiaao in vitro. C i?eaaaeaieo o oaaeeoe? 4 aeaieo aeaeii, ui ?ioaineai?noue neioaco e?i?ae?a o neaeaoieo i’ycao ei??a caeaaeeoue a?ae o?c?ieia?/iiai noaio ? a?eueoith i??ith a?ae ?o iiia?aaeieea. E?euee?nia noi?iia aeei?enoaiiy aeine?aeaeoaaieo e?iiaaiieo noano?ao?a o neioac? e?i?ae?a o neaeaoieo i’ycao ei??a ? oaeeoeue niaoeeo?/ia aeey eiaeiiai c ieo cie?aia. Oae, ?aae?iaeoeai?noue e?i?ae?a, neioaciaaieo aiiiaaiaoaie neaeaoieo i’yc?a ei??a a e?ioe? ia?oiai i?nyoey eaeoaoe?? i?e ?ieoaaoe?? c [1-14N] ioeoiaith ? [1-14N] noaa?eiiaith eeneioaie ieae/a, a i?e ?ieoaaoe?? c [6-14N] aetheicith ? D,L-3-oai?e-[1-14N]-aeai?iii - aeua, ii??aiyii aei ?aae?iaeoeaiino? e?i?ae?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca ei??a a e?ioe? nueiiiai i?nyoey eaeoaoe?? ? iniaeeai a e?ioe? o?eueiino?. Ine?eueee ioeoiaa eeneioa ? aeiaaieaioethaia? a?euei? aee?i? eeneioe aiinyoue iaea?eueoee aeeaae o noano?aoia caaacia/aiiy neioaco e?i?ae?a o oeaieiao aeoeieo, a oiio /ene? o iiei/i?e caeic?, oi c iaea?aeaieo iaie ?acoeueoao?a aeieeaa?, ui aeyaeaia ca?eueoaiiy aeei?enoaiiy [1-14N] ioeoiai? ? [1-14N] noaa?eiiai? eeneio o neioac? e?i?ae?a aiiiaaiaoaie neaeaoieo i’yc?a ei??a o e?ioe? eaeoaoe?? ? nooino?eiiai ia??iaeo iaoiiaeaia ciaioaiiyi ?o aeei?enoaiiy o neioac? e?i?ae?a iieiea. I?e oeueiio caa?oa? ia naaa oaaao ia?aeae?ci o ci?iao ?ioaineaiino? neioaco a?ee?a ? e?i?ae?a o neaeaoieo i’ycao ei??a i?e ci?iao ?o o?c?ieia?/iiai noaio, ui iaoiiaeaii aaaeeeaei cia/aiiyi a?ee?a ? oinoie?i?ae?a, ye no?oeoo?ieo eiiiiiaio?a ee?oei. I?aaenoaaeai? o oaaeeoe? 4 aeai? na?ae/aoue i?i aenieee nooi?iue aeei?enoaiiy a neioac? e?i?ae?a o neaeaoieo i’ycao ei??a a oiiaao in vitro ia o?eueee [1-14N] ioeoiai? ? [1-14N] noaa?eiiai? eeneio, a e D,L-3-oai?e-[1-14N]-aeai?io ? [6-14N] aetheice. ?aae?iaeoeai?noue e?i?ae?a, neioaciaaieo c aeacaieo iiia?aaeiee?a o neaeaoieo i’ycao ei??a ciaiuo?oueny o ?yae?: [1-14N] noaa?eiiaa eeneioa, [1-14N] ioeoiaa eeneioa, [6-14N] aetheica, D,L-3-oai?e-[1-14N]-aeai?i, ui na?ae/eoue i?i iniiaio ?ieue aeiaaieaioethaiaeo aee?ieo eeneio o neioac? Oaaeeoey 4. ?aae?iaeoeai?noue caaaeueieo e?i?ae?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca ei??a ? oaeeoeue i?ney ?ieoaaoe?? c ??cieie iiia?aaeieeaie (M±m, b-?ici./100ia ne?.oe./oa,n=3). Iiia?aaeieee E i ? i a e e?i?ae?a 1-e i?n.eaeo. 7-e i?n.eaeo. nooino?ei? [1-14N] ioeoiaa eeneioa 977±42 1090±54 1178±47* [6-14N] aetheica 615±27 524±24* 561±19 [1-14N] noaa?eiiaa eeneioa 2615±130 2878±116 3328±158* D,L-3-oai?e-[1-14N]-aeai?i 964±39 715±22* 752±44* o neaeaoieo i’ycao ei??a ? oaeeoeue. ?acii c oei, c iaea?aeaieo iaie ?acoeueoao?a aeieeaa?, ui aoeaoee-NiA o neaeaoieo i’ycao ei??a ooai?th?oueny ia o?eueee oeyoii aeoeaaoe?? ioeoiai? eeneioe, yea ? i?iaeoeoii ?oaoeaai? oa?iaioaoe?? ? i?ney aniieooaaiiy iiiaaea? o aioo??oiueiee?oeiiee ioe e?iiaaiieo noano?ao?a, a oaeiae a i?ioean? iaoaaie?cio aetheice ? ai?iieeneio, cie?aia, oai?eaeai?io. A ?acoeueoao? i?iaaaeaieo aeine?aeaeaiue anoaiiaeaii, ui ?ioaineai?noue neioaco ie?aieo eean?a e?i?ae?a o neaeaoieo i’ycao ei??a ? oaeeoeue caeaaeeoue, c iaeiiai aieo, a?ae iniaeeainoae iaoaaie?cio aeine?aeaeoaaieo e?iiaaiieo noano?ao?a, a c ae?oaiai - a?ae o?c?ieia?/iiai noaio oaa?ei. Oea iieiaeaiiy ?ethno?othoue aeai?, i?eaaaeai? o oaaeeoe? 5, c yeeo aeaeii, ui i?e ?ieoaaoe?? aiiiaaiao?a aeine?aeaeoaaiiai neaeaoiiai i’yca ei??a c [1-14N] ioeoiaith eeneioith 14,72-29,03% ?aae?iaeoeaii? i?oee aeyaeaii o oinoie?i?aeao, 6,14-15,03% - o iiii- ? aeeaoeeeae?oea?ieao, 9,53-20,14% - o o?eaoeeeae?oea?ieao, 23,03-31,89% - o iaaoa?eo?eiaaieo aee?ieo eeneioao, a o a?eueiiio ? aoa?eo?eiaaiiio oieanoa?ie? - a?aeiia?aeii 8,99-20,36 ? 8,04-13,00%. I?e ?ieoaaoe?? aiiiaaiao?a neaeaoiiai i’yca oaeeoeue c [1-14N] ioeoiaith eeneioith ?aae?iaeoeai?noue neioaciaaieo oinoie?i?ae?a, iiii- ? aeeaoeeeae?oea?ie?a, IAAEE, o?eaoeeeae?oea?ie?a, a?eueiiai ? aoa?eo?eiaaiiai oieanoa?ieo noaiiaeoue a?aeiia?aeii 20,14; 7,63; 23,09; 20,14; 13,43 ? 15,63% caaaeueii? ?aae?iaeoeaiino? aeacaieo eean?a e?i?ae?a. I?eaaaeai? aeai? na?ae/aoue i?i aenieo ?ioaineai?noue neioaco o neaeaoieo i’ycao ei??a ? oaeeoeue ia o?eueee oinoie?i?ae?a, ye? ? no?oeoo?ieie eiiiiiaioaie, a ? an?o eean?a iaeo?aeueieo e?i?ae?a, iniaeeai oieanoa?ieo ? o?eaoeeeae?oea?ie?a. Aenieee a?aeiiniee ai?no iaaoa?eo?eiaaieo aee?ieo eeneio o neaeaoieo i’ycao ei??a ? oaeeoeue, neioaciaaieo c ioeoiai? Oaaeeoey 5. ?aae?iaeoeai?noue ie?aieo eean?a e?i?ae?a, neioaciaaieo aiiiaaiaoaie neaeaoiiai i’yca oaeeoeue, eaeooth/eo ? nooino?eieo ei??a i?e ?ieoaaoe?? c [1-14N] ioeoiaith eeneioith (M±m, n=3). Eeane e?i?ae?a Ei?iae Oaeeoe? 1-ee i?n. eaeo. 7-ee i?n. eaeo. nooino?ei? Oinoie?i?aee, a a 144±4,7 14,72 292±10,5* 26,71 342±12,9* 29,03 174±4,5* 20,14 Iiii- ? aeeaoeee- a ae?oea?iee, a 60±1,9 6,14 102±4,3* 9,30 177±8,4* 15,03 66±2,1 7,63 A?eueiee a oieanoa?ie a 199±6,6 20,36 96±3,9* 8,99 152±4,2* 12,93 116±6,1* 13,43 IAAEE a a 312±8,2 31,89 309±10,4 28,31 300±12,6 25,45 199±5,4* 23,09 O?eaoeee- a ae?oea?iee a 135±4,9 13,81 193±7,2* 17,70 112±4,4 9,53 174±8,3* 20,14 Aoa?eo?eiaaiee a oieanoa?ie a 127±5,1 13,00 98±3,3* 8,99 95±4,5* 8,03 135±5,0 15,63 I?ei?oea: a - ?aae?iaeoeai?noue (b-?ici./100ia ne?.oe./oa), a - % a?ae caaaeueii? ?aae?iaeoeaiino?. eeneioe na?ae/eoue i?i a?aeiinii iecueeee nooi?iue ?o aeei?enoaiiy o i?ioeanao aoeeethaaiiy i?e neioac? e?i?ae?a. Io?eiai? aeai? (oaae. 5) na?ae/aoue i?i cia/iee aieea o?c?ieia?/iiai noaio ia ?ioaineai?noue neioaco aeayeeo eean?a e?i?ae?a o neaeaoieo i’ycao ei??a. Cie?aia, a?aeiinia ?ioaineai?noue neioaco a?eueiiai ? aoa?eo?eiaaiiai oieanoa?ieo c [1-14N] ioeoiai? eeneioe o neaeaoieo i’ycao ei??a ia ii/aoeo eaeoaoe?? aeua, a ?ioaineai?noue neioaco oinoie?i?ae?a ? o?eaoeeeae?oea?ie?a, iiii- ? aeeaoeeeae?oea?ie?a - ieae/a, i?ae a e?ioe? eaeoaoe??. Oe? ??cieoe? iiaeia iiynieoe iecueeith ?ioaineai?noth neioaco a?ee?a o neaeaoieo i’ycao ei??a ia ii/aoeo eaeoaoe??, ine?eueee neioac a?ee?a ? oinoie?i?ae?a o oeaieiao oaa?ei i?e ae?? ai?iii?a noeioeth?oueny iaeiaeiaith i??ith [Tata J.R., 1972]. Iineeaiiy neioaco oinoie?i?ae?a o neaeaoieo i’ycao ei??a a e?ioe? eaeoaoe?? ? a nooino?eiee ia??iae ei?aeth? c aeyaeaiei iaie iineeaiiyi neioaco a?ee?a, ui aeaco? ia iayai?noue iaaiiai ca’yceo i?ae aeacaieie i?ioeanaie, ye? ca’ycai? c a?aeiiaeaiiyi aeeai? iane ei??a ? ?inoii ieiaea. I?e oeueiio caa?oa? ia naaa oaaao cieaeaiiy ?ioaineaiino? neioaco o?eaoeeeae?oea?ie?a o neaeaoieo i'ycao ei??a o e?ioe? o?eueiino?, ui iaoiiaeaii iineeaiiyi ?o neioaco o aee?ia?e oeaiei? [Iaothuaiei I.A., 1992]. Aieea o?c?ieia?/iiai noaio ia aia?aaoe/i? i?ioeane o neaeaoieo i’ycao ei??a ? oaeeoeue a oiiaao in vitro. Aeeaae aeine?aeaeoaaieo aia?aaoe/ieo noano?ao?a o caaacia/aiiy iio?aae neaeaoieo i’yc?a ei??a ? oaeeoeue a iaoaaie?/i?e aia?a?? ciaioo?oueny a ?yae?: [1-14N] noaa?eiiaa eeneioa, [1-14N] ioeoiaa eeneioa, [6-14N] aetheica, D,L-3-oai?e-[1-14N]-aeai?i. I?e oeueiio a?aoiaaii, ui a i?ioean? ieeniaiiy iieaeoee [1-14N] noaa?eiiai? ? [1-14N] ioeoiaa eeneio, [6-14N] aetheice ? D,L-3-oai?e-[1-14N]-aeai?io ooai?th?oueny a?aeiia?aeii 9, 1, 2 ? 2 iieaeoee aoeaoee-NiA, c yeeo eeoa iaeia iieaeoea i?/aia ?aae?iaeoeaiei aoaeaoeai. C oeeo aeaieo aeieeaa?, ui aeiaaieaioethaia? aee?i? eeneioe aiinyoue cia/ii a?eueoee aeeaae o noano?aoia caaacia/aiiy aia?aaoe/ieo i?ioean?a o neaeaoieo i’yc?a ei??a ? oaeeoeue, i?ae ?io? aia?aaoe/i? noano?aoe, cie?aia, ioeoiaa eeneioa, aetheica ? ai?iieeneioe. I?iaeoeoe?y NI2 ca ?aooiie ieeniaiiy aeine?aeaeoaaieo aia?aaoe/ieo noano?ao?a, ca aeiyoeii oai?eaeai?io, o neaeaoieo i’ycao ei??a a e?ioe? eaeoaoe?? ? iniaeeai o nooino?eiee ia??iae cia/ii a?eueoa, i?ae ia ii/aoeo eaeoaoe??. Cie?aia, i?iaeoeoe?y NI2 ca ?aooiie ieeniaiiy [1-14N] ioeoiai? eeneioe, [6-14N] aetheice ? [1-14N] noaa?eiiai? eeneioe o neaeaoieo i’ycao nooino?eieo ei??a a?aeiia?aeii a 1,45; 1,34 ? 1,27 ?ace a?eueoa, i?ae o neaeaoieo i’ycao ei??a ia ia?oiio i?nyoe? eaeoaoe??. I?aeaeuaiiy aeei?enoaiiy aeacaieo noano?ao?a a aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao Oaaeeoey 6 - ?aae?iaeoeai?noue NI2, ooai?aiiai aiiiaaiaoaie neaeaoiiai i’yca oaeeoeue, eaeooth/eo ? nooino?eieo ei??a i?e ?ieoaaoe?? c ??cieie aia?aaoe/ieie noano?aoaie (M±m, b-?ici./100ia ne?.oe./oa,n=3). Aia?aaoe/i? Ei?iae Oaeeoe? noano?aoe 1-ei?n.eaeo. 7-ee i?n. aeo. nooino?ei? [1-14N] ioeoiaa eeneioa 234±6,3 302±10,9* 341±9,7* 321±11,4* [6-14N] aetheica 119±3,8 136±4,5* 160±5,9* 170±3,6* [1-14N] noaa?eiiaa eeneioa 197±5,9 224±5,0* 324±10,4* 297±8,2* D,L-3-oai?e-[1-14N]- aeai?i 130±5,2 135±4,3 126±6,1 147±5,4* ei??a o nooino?eiee ia??iae iiaeia iiynieoe aeyaeaiei iaie i?aeaeuaiiyi o oe?e oeaiei? ?ioaineaiino? neioaco a?ee?a ? e?i?ae?a. Oea iaoiiaeaii oei, ui neioac a?ee?a ? e?i?ae?a o oeaieiao oaa?ei - aia?aicaeaaei? i?ioeane, a yeeo aeei?enoiao?oueny aia?a?y AOO, ui ocaiaeaeo?oueny c aeyaeaiith iaie aenieith i?iaeoeoe??th 14NI2 ca ?aooiie ieeniaiiy an?o aeine?aeaeoaaieo aia?aaoe/ieo noano?ao?a o neaeaoieo i’ycao oaeeoeue, o yeeo aeyaeaiee iaea?eueo ?ioaineaiee neioac a?ee?a. Ioaea, ?ioaineai?noue aia?aaoe/ieo i?ioean?a o neaeaoieo i’ycao ei??a a oiiaao in vitro cia/iith i??ith caeaaeeoue a?ae ?o o?c?ieia?/iiai noaio. Aieea oaeoi??a aeeaeaiiy ia iaoaaie?/iee i?io?eue o e?ia? ei??a, canai?iiy iiaeeaieo ?a/iaei ei?io ? aeei?enoaiiy ?o o neioac? eiiiiiaio?a iieiea. O ?acoeueoao? i?iaaaeaieo aeine?aeaeaiue anoaiiaeaii, ui caoaeaiiy a?aeiioaiiy i?ae ai?noii oeoe?o ? e?ioiaeth a ?aoe?ii? ei??a c 1:1,6 (ia?oee ia??iae) aei 1:1,1 (ae?oaee ia??iae) i?eaaei aei ca?eueoaiiy ia?ao?aaiino? ne?iai i?ioa?io c 66,9 aei 71,1% ? iacia/iiai cieaeaiiy ia?ao?aaiino? nooi? ?a/iaeie, ee?oeiaeie, ne?iai aee?o, AA? a ?aoe?ii?.. O ai?noeiiio ?oaoey ei??a a ae?oaiio ia??iae?, ii??aiyii aei ia?oiai, aeyaeaia a?eueoa eiioeaio?aoe?y a?eeiaiai acioo, eaoeeo aee?ieo eeneio ? oeoe??a oa iaioa eiioeaioaoe?y ai?aeo ? iaa?eeiaiai acioo (?<0,05-0,001). Oe? aeai? na?ae/aoue i?i ?nioaaiiy ca'yceo, c iaeiiai aieo, i?ae i?ioeanaie oa?iaioaoe?? iayaieo o ei?iao aoaeaaiae?a, a ?acoeueoao? yei? ooai?ththoueny iecueeiiieaeoey?i? aee?i? eeneioe (EAEE), a c ae?oaiai - i?ae neioacii aaeoa??aeueiiai i?ioa?io. Ci?ia a?aeiioaiiy oeoe?o aei e?ioiaeth a ?aoe?ii? ei??a cia/iith i??ith aieeioea ia ai?no aeayeeo aeine?aeaeoaaieo iaoaaie?o?a o e?ia? ? nooiaiy ?o iiaeeiaiiy iiei/iith caeicith, i?i ui na?ae/aoue aeyaeai? iaie ??cieoe? o ?o eiioeaio?aoe?? a e?ia? /a?aaii? ai?oe ? iiei/ii? aaie. Cie?aia, o e?ia? /a?aaii? ai?oe ei??a aeyaeaii a?eueoee ai?no a?ee?a, eaoiiiaeo o?e, ioeoiai? eeneioe, aetheice ? iaioee - caeeoeiaiai acioo ? na/iaeie (?<0,05-0,001). Cieaeaiiy eiioeaio?aoe?? inoaii?o iaoaaie?o?a aeaco? ia a?eueoo ?aoaioe?th acioo a i?aai?ci? ei??a i?e ca?eueoaii? /anoee oeoe?o a ?o ?aoe?ii?. I?e oeueiio niinoa??aaeiny cia/ii a?eueoa iiaeeiaiiy iiei/iith caeicith a?ee?a, aetheice, IAAEE, ioeoiai? eeneioe (?<0,05-0,01), ui iaoiiaeei i?aeaeuaiiy ai?noo aee?o ? eaeoice a iieioe? a?aeiia?aeii ia 9,7 ? 10,8% . Iaea?aeai? iaie ?acoeueoaoe na?ae/aoue i?i a?eueo aoaeoeaia aeei?enoaiiy oeoe?o, i?ae e?ioiaeth a aia?aaoe/ieo i?ioeanao i?e?ii?aai?ciaie a ?oaoe? ? ca?eueoaiiy i?iaeoeoe?? iaoaaie?o?a - iiia?aaeiee?a neioaco eiiiiiaio?a iieiea. O o?aoueiio ia??iae? ca?eueoaiiy /anoee aaaeei?icuaiethaaiiai i?ioa?io o ?aoe?ii? ei??a i?eaiaeeei aei ca?eueoaiiy eiao?oe??ioo canai?iiy ne?iai i?ioa?io ia 14,5%. Iieacaii, ui aeeth/aiiy a ?aoe?ii ei??a "caoeuaii?" iaeooe aieeaaei ia ai?no aeinooiiiai aeey canai?iiy i?ioa?io ? neioac aaeoa??aeueiiai ?ioa?io a ?oaoe? [Ei??iaoeue TH. ss. ? ?i., 1997]. I?e oeueiio o iinoi?ei?e/iiio o?ion? niinoa??aaeiny c?inoaiiy e?eueeino? ne?iai i?ioa?io. Ca?eueoaiiy iinooieaiiy inoaiiueiai a 12-iaeo eeoeo iaoiiaeaii ciaioaiiyi ?icuaieaiiy i?ioa?io a ?oaoe?. I?e niiaeeaaii? ei?iaaie ei?i?a c a?eueo iecueeith ?icuaiethaai?noth i?ioa?io o ai?noeiiio ?oaoey ciaioo?oueny ??aaiue ai?aeo ? iaa?eeiaiai acioo (?<0,05), a e?ia? cieaeo?oueny eiioeaio?aoe?y caeeoeiaiai acioo (c 31,61 aei 29,79 ia%) ? na/iaeie (c 14,74 aei 13,19 ia%), ui na?ae/eoue i?i e?aua aeei?enoaiiy acioo oaa?eiaie. Eiao?oe??io canai?iiy nooi? ?a/iaeie o o?aaiiio eaiae? ei??a o o?aoueiio ia??iae? aoa iaioee ia 4,5%, ne?i? ee?oeiaeie - ia 1,0%, ne?iai aee?o – ia 2,9%, AA? – ia 2,7%, i?ae o ei??a o ia?oiio ia??iae?. Oea iiynith?oueny cieaeaiiyi ?ioaineaiino? oa?iaioaoeaieo i?ioean?a o ?oaoe? ei??a o o?aoueiio ia??iae? aiane?aeie ciaioaiiy a ?o ?aoe?ii? /anoee eaaei?icuaiethaaiiai i?ioa?io, yeee ? iniiaiei aeaea?aeii acioo aeey ?inoo i?e?ii?aai?ci?a o ?oaoe? ? neioaco aaeoa??aeueiiai i?ioa?io [Leng R.A., 1984; Bull L.S., 1985], ? i?eai?/aiiyi neioaco i?e?iaiiai i?ioa?io i?e canoinoaaii? oi?iaeueaeaa?aeo aeey caoenoo ei?iiaiai a?eea. I?e cieaeaii? /anoee eaaei?icuaiethaaiiai i?ioa?io a ?aoe?ii? ei??a aeyaeaii iacia/ia ca?eueoaiiy eiioeaio?aoe?? eaoeeo aee?ieo eeneio o ai?noeiiio ?oaoey ? eiioeaio?aoe?? ioeoiai? eeneioe a e?ia?. Iiaeeiaiiy ?? iiei/iith caeicith ei??a ii??aiyii c eiio?ieueiei ia??iaeii aoei aeuei ia 28%. Ai?no aee?o a iieioe? ei??a i?e oeueiio c?inoaa ia 20% , eaeoice - ia 16%. C iaea?aeaieo ?acoeueoao?a aeieeaa?, ui niiaeeaaiiy ei?iaaie ei?iiaiai i?ioa?io, caoeuaiiai a?ae iaaei??iiai ?icuaieaiiy a ?oaoe? i?eaiaeeoue aei a?eueoiai iinooieaiiy eiai a oiieee eeoa/iee ? a?eueo aoaeoeaiiai eiai aeei?enoaiiy. AENIIAEE 1. A ?acoeueoao? i?iaaaeaieo aeine?aeaeaiue iaea?aeaii iia? aeai? i?i aieea o?c?ieia?/iiai noaio (noaae?? eaeoaoe?? ? aaa?oiino?) ? oeio aiae?ae? ia iaoaaie?/iee i?io?eue o e?ia?, neioaoe/i? ? aia?aaoe/i? i?ioeane o neaeaoieo i’ycao ei??a, i?i aieea ??ciiai a?aeiioaiiy i?ae ai?noii oeoe?o ? e?ioiaeth, eaaei- ? aaaeei?icuaiethaaiiai i?ioa?io a ?aoe?ii? ei??a ia iaoaaie?/iee i?io?eue e?ia?, oa?iaioaoeai? i?ioeane o ?oaoe?, canai?iiy iiaeeaieo ?a/iaei ei?io ? aeei?enoaiiy ??cieo iiia?aaeiee?a iieiea o neioac? eiai eiiiiiaio?a o iiei/i?e caeic? ei??a, ye? aoaeooue aeei?enoai? i?e ?ic?iaoe? iiai? nenoaie ?o aeeaeaiiy ? eiio?ieth ca o?c?ieia?/iei noaiii. 2. Iaoaaie?/iee i?io?eue a e?ia? ei??a cia/iith i??ith caeaaeeoue a?ae ?o o?c?ieia?/iiai noaio, oeio aiae?ae? ? neeaaeo ?aoe?iio. Iaea?eueoa ci?ith?oueny a e?ia? ei??a o caeaaeiino? a?ae aeacaieo oaeoi??a eiioeaio?aoe?y caaaeueieo e?i?ae?a, o?eaoeeeae?oea?ie?a, aetheice ? na/iaeie (?<0,05-0,001). 3. ?ioaineai?noue neioaco a?ee?a a oiiaao in vitro o neaeaoieo i'ycao ei??a ia ii/aoeo eaeoaoe?? cia/ii ieae/a, i?ae o e?ioe? ? a nooino?eiee ia??iae, ? iniaeeai o neaeaoieo i'ycao 18-i?ny/ieo oaeeoeue. ?ioaineai?noue neioaco a?ee?a o neaeaoieo i'ycao eaeooth/eo ei??a a oiiaao in vitro aeicicaeaaeii i?aeaeuo?oueny i?e aeiaeaaaii? aei ?ieoaaoe?eiiai na?aaeiaeua ?inoe?io o aenieeo aeicao. 4. Aeoeai?noue iaeo?aeueieo i?ioaac o neaeaoieo i'ycao ei??a ia ii/aoeo eaeoaoe?? aeua, i?ae a e?ioe? ? a nooino?eiee ia??iae, oiae? ye ??cieoey a aeoeaiino? eeneeo i?ioaac i?e oeueiio a?aenooiy. Aeoeai?noue iaio i?ioaac o neaeaoieo i'ycao 18-i?ny/ieo oaeeoeue a 1,5-2 ?ace aeua, i?ae o neaeaoieo i'ycao eaeooth/eo ? o?eueieo ei??a. 5. ?ioaineai?noue neioaco e?i?ae?a o neaeaoieo i'ycao ei??a a oiiaao in vitro i?e aeei?enoaii? ye iiia?aaeieea [1-14N] noaa?eiiai? ? [1-14N] ioeoiai? eeneio cia/ii ieae/a, a i?e aeei?enoaii? D,L-3-oai?e-[1-14N]-aeai?io ? [6-14N] aetheice - aeua ia ii/aoeo eaeoaoe??, i?ae o e?ioe? ? a nooino?eiee ia??iae. 6. O neaeaoieo i'ycao ei??a a oiiaao in vitro [1-14N] noaa?eiiaa eeneioa cia/ii a?eueoith i??ith aeei?enoiao?oueny a neioac? e?i?ae?a, i?ae [1-14N] ioeoiaa eeneioa, [6-14N] aetheica ? D,L-3-oai?e-[1-14N]-aeai?i. 7. ?ioaineai?noue ieeniaiiy [6-14N] aetheice, [1-14N] ioeoiai? ? [1-14N] noaa?eiiai? eeneio a aia?aaoe/ieo i?ioeanao o neaeaoieo i'ycao ei??a a oiiaao in vitro ia ii/aoeo eaeoaoe?? cia/ii ieae/a, i?ae a e?ioe? ? iniaeeai o nooino?eiee ia??iae. 8. A aia?aaoe/ieo i?ioeanao o neaeaoieo i'ycao eaeooth/eo ? nooino?eieo ei??a a oiiaao in vitro [1-14N] noaa?eiiaa ? [1-14N] ioeoiaa eeneioe aeei?enoiaothoueny cia/ii a?eueoith i??ith, i?ae [6-14N] aetheica ? 3-oai?e-[1-14N]-aeai?i. 9. Ci?ia ni?aa?aeiioaiiy i?ae ai?noii oeoe?o ? e?ioiaeth a ?aoe?ii? eaeooth/eo ei??a c 1:1,6 aei 1:1,1 i?eaiaeeoue aei ca?eueoaiiy ia?ao?aaiino? i?ioa?io ? ciaioaiiy ia?ao?aaiino? ee?oeiaeie, aei ca?eueoaiiy i?iaeoeoe?? EAEE ? neioaco aaeoa??aeueiiai i?ioa?io a ?oaoe? ? a?eueoiai iiaeeiaiiy ioeoiai? eeneioe ? aetheice iiei/iith caeicith, aei ca?eueoaiiy ai?noo aee?o ? eaeoice a iieioe?. 10. Ca?eueoaiiy /anoee aaaeei?icuaiethaaiiai i?ioa?io a ?aoe?ii? eaeooth/eo ei??a c 29 aei 41% i?eaiaeeoue aei ca?eueoaiiy ia?ao?aaiino? i?ioa?io a eeoa/ieeo, aei i?aeaeuaiiy eiioeaio?aoe?? aetheice, ioeoiai? eeneioe ? eaoiiiaeo o?e a e?ia?, aei ca?eueoaiiy ai?noo aee?o ? eaeoice a iieioe?. NIENIE ?IA?O, IIOAE?EIAAIEO CA OAIITH AeENA?OAOe?? 1. Ei??iaoeue TH.ss., *a?e?i A.A., O???anueeee I.?., Iieioeei I.N., Aeiaeee ?.C. Aieea cieaeaiiy ??aiy eaaei?icuaiethaaiiai i?ioa?io a ?aoe?ii? ei??a ia i?ioeane o?aaeaiiy ? canai?iiy iiaeeaieo ?a/iaei ei?i?a // Iaoe.-oaoi. athe. ?ino. o?c?ie. ? a?io?i. o-i.- 1997.- Aei. 19 (1).- N. 78-81. (Aeena?oaio a?aa o/anoue o iinoaiiaoe? aeine?aeo, a?aeai?? iaoa??aeo, aein-e?aeaeaii? a?io?i?/ieo iieaciee?a e?ia?, aiae?c? iaea?aeaieo aeaieo). 2. Iieioeei I.N., Ei??iaoeue TH.ss., ssiiae/ A.A. Aieea o?c?ieia?/iiai noaio ei??a ia aia?aaoe/i? i?ioeane o neaeaoieo i’ycao // Ia?aaea??ia oa a??nueea caiea?ianoai ? oaa?eiieoeoai.- 1999.- ? 40-41.- N. 118-119. (Aeena?oaio i?ia?a aenia?eiaioaeuei? aeine?aeaeaiiy, noaoenoe/io ia?iaeo, aiae?c ? iienaiiy io?eiaieo aeaieo). 3. Iieioeei I.N., Ei??iaoeue TH.ss., Iaiooo O.A. Iaoaaie?/iee i?io?eue e?ia? ei??a ca ??ciiai o?c?ieia?/iiai noaio // A?niee A?eioea?e?anueeiai aea?aeaaiiai aa?. Oi-oo.- Aeione 5.-*ano. 1.- A?ea Oea?eaa.- 1998.- N. 217-218. (Aeena?oaio i?ia?a aenia?eiaioaeuei? aeine?aeaeaiiy, aeine?aeea a?io?i?/i? iieacieee e?ia?, i?ia?a noaoenoe/io ia?iaeo, aiae?c ? iienaiiy iaea?aeaieo aeaieo). 4. Iieioeei I.N., Ei??iaoeue TH.ss., ssiiae/ A.A. Aieea o?c?ieia?/iiai noaio ei??a ia neioac a?ee?a ? e?i?ae?a o neaeaoieo i’ycao // A?ieia?y oaa?ei.- 1999.- ?1.- N. 56-59. Aeena?oaio i?ia?a aenia?eiaioaeuei? aeine?aeaeaiiy, noaoenoe/io ia?iaeo ? aiae?c io?eiaieo aeaieo. 5. Iieioeei I.N., ssiiae/ A.A. Aieea ?inoe?io ia neioac a?ee?a o neaeaoieo i’ycao eaeooth/eo ei??a ? oaeeoeue a oiiaao in vitro // Ca. noaoae i?aeia?. iaoe.-i?aeo. eiio. “No/ani? i?iaeaie a?ieia??, aaoa?eia?ii? iaaeeoeeie, cii?iaeaia??? oa oaoiieia?e i?iaeoeo?a oaa?eiieoeoaa”.- Euea?a, 9-11 aeiaoiy 1997.- N. 376-378. Aeena?oaio i?ia?a aenia?eiaioaeueio /anoeio ?iaioe, noaoenoe/io ia?iaeo io?eiaieo aeaieo ? ?o iienaiiy. 6. Iieioeei I.N., Iaiooo O.A., Ei??iaoeue TH.ss., ssiiae/ A.A. Aieea a?eo ? o?c?ieia?/iiai noaio ia neioac ? ?iciaae a?ee?a o neaeaoieo i’ycao aaeeei? ?iaaoi? ooaeiae // ??? Iaaeaeoia?. neii. “Aeieiae/aneea iaoaieciu noa?aiey”.- Oa?ueeia.-1998.- N. 25. 7. Ei??iaoeue TH.ss., *a?e?i A.A., O???anueeee I.?., Iieioeei I.N., Aeiaeee ?.C. Aeei?enoaiiy aia?a?? ia ??ci? ooieoe?? a i?aai?ci? ei??a i?e cieaeaii? ?icuaieaiiy i?ioa?io ?aoe?iio // XV c’?cae Oe?. o?c?ie. o-aa.: Oace aeii.- O?c?ie. aeo?i.- 1998.- O. 44,? 3.- N. 231-232. 8. O.S. Pocotylo., V.G. Janovich. Influence of physiological status on metabolic profile in cows blood // XVII Conf. ”Dni fyziologie hospodarskych zvierat”. Abstrakty.- Ko?ice, 12-14 november 1997.- P. 61. 9. Iaiooo O.A., Iieioeei I.N., Ei??iaoeue TH.ss., ssiiae/ A.A. Aieea neeiniiai ? n?iaaeiiai oei?a aiae?ae? ia iaoaaie?/iee i?io?eue a e?ia? ei??a // Oace aeii. i?aeia?. eiio. “A?ieia?/i? iniiae aeeaeaiiy n?euenueeiainiiaea?nueeeo oaa?ei”.- Euea?a.- 1998.- N. 42. 10. Iieioeei I.N., Ei??iaoeue TH.ss., ssiiae/ A.A. Aieea ?inoe?io ia aeei?enoaiiy [1-14N] oai?eaeai?io a neioac? a?ee?a ? aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao ei??a ca ??ciiai o?c?ieia?/iiai noaio // XV c’?cae Oe?. o?c?ie. o-aa.: Oace aeii.- O?c?ie. aeo?i.- 1998.- O. 44,? 3.- N. 235-236. Iieioeei I.N. Aieea o?c?ieia?/iiai noaio ? oaeoi??a aeeaeaiiy ia iai?i ?a/iaei a i?aai?ci? ei??a.- ?oeiien. Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe ca niaoe?aeuei?noth 03.00.04 - a?io?i?y.- ?inoeooo caiea?ianoaa ? a?ieia?? oaa?ei OAAI, Euea?a, 1999. O aeena?oaoe?? i?eaaaeai? aeai? i?i ci?ie iaoaaie?/iiai i?io?eth e?ia?, ?ioaineaiino? neioaoe/ieo ? aia?aaoe/ieo i?ioean?a o neaeaoieo i’ycao ei??a a caeaaeiino? a?ae o?c?ieia?/iiai noaio ? oaeoi??a aeeaeaiiy. Cie?aia, anoaiiaeaii caeaaei?noue ai?noo caaaeueiiai a?eea ? ni?aa?aeiioaiiy ie?aieo o?aeoe?e, caaaeueieo e?i?ae?a, o?eaoeeeae?oea?ie?a, iaaoa?eo?eiaaieo aee?ieo eeneio, aetheice ? na/iaeie a e?ia? ei??a a?ae ?o o?c?ieia?/iiai noaio, oeio aiae?ae? ? neeaaeo ?aoe?iio. Anoaiiaeaii oaeiae, ui ?ioaineai?noue neioaco a?ee?a, e?i?ae?a ? aia?aaoe/ieo i?ioean?a o neaeaoieo i’ycao ei??a ia ii/aoeo eaeoaoe?? ieae/a, i?ae o e?ioe? ? iniaeeai o nooino?eiee ia??iae. Aeyaeai? ?noioi? ??cieoe? o nooiai? aeei?enoaiiy noaa?eiiai? ? ioeoiai? eeneio, aetheice ? oai?eaeai?io o noano?aoiiio caaacia/aii? neioaco e?i?ae?a ? aia?aaoe/ieo i?ioeanao o neaeaoieo i’ycao ei??a ia ii/aoeo ? a e?ioe? eaeoaoe??, a oaeiae o e?ioe? o?eueiino?. Iieacaii, ui ca?eueoaiiy /anoee oeoe?o ? aaaeei?icuaiethaaiiai i?ioa?io o ?aoe?ii? ei??a aieeaa? ia oa?iaioaoeai? i?ioeane ? neioac aaeoa??aeueiiai i?ioa?io o ?oaoe?, ia iiaeeiaiiy iiei/iith caeicith iiia?aaeiee?a iieiea, ia ca?eueoaiiy ai?noo aee?o ? eaeoice a iieioe?. Ca?eueoaiiy /anoee aaaeei?icuaiethaaiiai i?ioa?io a ?aoe?ii? eaeooth/eo ei??a i?eaiaeeoue aei ca?eueoaiiy ia?ao?aaiino? ? canai?iiy i?ioa?io, aei ca?eueoaiiy eiioeaio?aoe?? aetheice, ioeoiai? eeneioe ? eaoiiiaeo o?e a e?ia? ? ?o aoaeoeai?oiai aeei?enoaiiy a neioac? eiiiiiaio?a iieiea a iiei/i?e caeic?. Eeth/ia? neiaa: ei?iae, aeeaeaiiy, eaeoaoe?y, o?euei?noue, iaoaaie?/iee i?io?eue e?ia?, neaeaoi? i’yce, neioac, a?eee, e?i?aee, NI2. Iieioeei I.N. Aeeyiea oeceieiae/aneiai ninoiyiey e oaeoi?ia ieoaiey ia iaiai aauanoa a i?aaiecia ei?ia.- ?oeiienue. Aeenna?oaoeey ia nieneaiea iao/iie noaiaie eaiaeeaeaoa naeueneioicyenoaaiiuo iaoe ii niaoeeaeueiinoe 03.00.04 - aeioeiey. - Einoeooo caieaaeaeey e aeieiaee aeeaioiuo OAAI, Eueaia, 1999. A aeenna?oaoeee i?aaenoaaeaiu aeaiiua ia eciaiaieyo iaoaaiee/aneiai i?ioeey a e?iae, neioaoe/aneeo e yia?aaoe/aneeo i?ioeannia o neaeaoiuo iuooeao ei?ia a ia/aea e eiioea eaeoaoeee, e a eiioea nooinoieiiai ia?eiaea, i aeeyiee ia ieo oeia ei?ieaiey e ninoaaa ?aoeeiia, i aeeyiee ?aciiai niioiioaiey iaaeaeo eiee/anoaii eaaei - e o?oaeii?anuaieyaiiai i?ioaeia, naoa?a e e?aoiaea a ?aoeeiia eaeoe?othueo ei?ia ia eo i?iaeoeoeaiinoue, onaiaiea ieoaoaeueiuo aauanoa ei?ia, niaea?aeaiea a e?iae iniiaiuo i?aaeoanoaaiieeia eiiiiiaioia iieiea e eo iiaeiuaiea iiei/iie aeaeacie. Onoaiiaeaii, /oi oeceieiae/aneia ninoiyiea ei?ia, oei ei?ieaiey e ninoaa ?aoeeiia aeeyao ia niaea?aeaiea eeieaeia, o?eaoeeeaeeoea?ieia, iayoa?eoeoee?iaaiuo aee?ieo eeneio, aetheicu e ii/aaeiu a ieacia e?iae. Onoaiiaeaii oaeaea, /oi a ia/aea eaeoaoeee oae iacuaaaiue “yooaeo naeaeaaiey” ei?ia iaoneiaeai, n iaeiie noi?iiu, iiauoaieai aeoeaiinoe iaeo?aeueiuo i?ioaeiac, eioi?ua eaoaaieece?otho ?aniaae ieioea?eey?iuo aaeeia, n ae?oaie - nieaeaieai eioaineaiinoe neioaca aaeeia a neaeaoiuo iuooeao. A eiioea eaeoaoeee e nooinoieiiai ia?eiaea eioaineaiinoue neioaca aaeeia o neaeaoiuo iuooeao ei?ia iiauoaaony, a aeoeaiinoue iaeo?aeueiuo i?ioaeiac nieaeaaony, aeaaiaea?y /aio iaania/eaaaony ainnoaiiaeaiea iannu iuoa/iie oeaie e aeeaie iannu . Eioaineaiinoue neioaca eeieaeia o neaeaoiuo iuooeao ei?ia ec [1-14N] oenoniie e [1-14N] noaa?eiiaie eeneio o neaeaoiuo iuooeao ei?ia a caaeneiinoe io eo oeceieiae/aneiai ninoiyiey eciaiyaony ia?aeeaeueii eciaiaieyi eioaineaiinoe neioaca aaeeia, /oi iaoneiaeaii aaaeiie no?oeoo?iie ?ieueth oinoieeieaeia, eciaiaiey eioaineaiinoe neioaca eioi?uo ei??aee?otho n eciaiaieyie neioaca iuoa/iuo aaeeia. Eniieueciaaiea enneaaeoaiuo i?aaeoanoaaiieeia a neioaca eeieaeia neaeaoiuo iuooe ei?ia oiaiueoaaony a ?yaeo: noaa?eiiaay eeneioa, oenoniay eeneioa, oaieeaeaiei, aetheica. I?e yoii noaa?eiiaay e oenoniay eeneiou a cia/eoaeueii aieueoae noaiaie eniieuecothony a neioaca eeieaeia o neaeaoiuo iuooeao ei?ia, /ai aetheica e oaieeaeaiei. Eciaiaiea eioaineaiinoe yia?aaoe/aneeo i?ioeannia o neaeaoiuo iuooeao ei?ia i?e eciaiaieyo oeceieiae/aneiai ninoiyiey iaoiaeyony a i?yiie caaeneiinoe io eioaineaiinoe neioaca aaeeia, eioi?ua yaeythony yia?aaoe/anee caaeneiuie i?ioeannaie. I?e yoii naeaeaii caeeth/aiea, /oi noaa?eiiaay e oenoniay eeneiou a cia/eoaeueii aieueoae noaiaie eniieuecothony a yia?aaoe/aneeo i?ioeannao o neaeaoiuo iuooeao ei?ia, /ai aetheica e oaieeaeaiei. A ?acoeueoaoa i?iaaae?iiuo enneaaeiaaiee oaeaea onoaiiaeaii, /oi oaaee/aiea ioiineoaeueiiai eiee/anoaa naoa?a e o?oaeii?anuaieyaiiai i?ioaeia a ?aoeeiia eaeoe?othueo ei?ia i?eaiaeeo e iiauoaieth ia?aaa?eiinoe i?ioaeia, aai onaiaiea. Yoi aeeyao ia i?iaeoeoeeth ieceiiieaeoey?iuo aee?iuo eeneio e neioac aaeoa?eaeueiiai i?ioaeia a ?oaoea, ia iiaeiuaiea aetheicu e oenoniie eeneiou iiei/iie aeaeacie ia iiei/ioth i?iaeoeoeaiinoue ei?ia. I?e yoii a iieiea ei?ia oaaee/eaaaony niaea?aeaiea aee?a e eaeoicu. Iieo/aiiua ?acoeueoaou naeaeaoaeuenoaotho i aeeyiee oeia ei?ieaiey e ninoaaa ?aoeeiia ia iaoaaiee/aneee i?ioeeue a e?iae ei?ia, /oi iaiaoiaeeii o/eouaaoue a enneaaeiaaieyo aai i?e ?aciii oeceieiae/aneii ninoiyiee, i aeeyiee eciaiaiey niioiioaiey iaaeaeo niaea?aeaieai eaaei- e o?oaeii?anuaieyaiiai i?ioaeia, naoa?a e e?aoiaea a ?aoeeiia ei?ia ia oa?iaioaoeeth a ?oaoea, niaea?aeaiea i?aaeoanoaaiieeia eiiiiiaioia iieiea a e?iae e eo iiaeiuaiea iiei/iie aeaeacie, ninoaa iieiea. Eeth/aaua neiaa: ei?iau, ieoaiea, eaeoaoeey, noaeueiinoue, iaoaaiee/aneee i?ioeeue e?iae, neaeaoiua iuooeu, neioac, aaeee, eeieaeu, NI2. Pokotylo O.S. The influence of physiological state and nutritional factors on metabolism in cows organism. Manuscript. Thesis for a scientific degree of candidate of agriculture sciences by speciality 03.00.04- biochemistry.- Institute of agriculture and animal biology UAAS, L’viv, 1999. The data about changes of metabolic profile of blood, intensity of synthetically and energetically processes in skeletal muscles depending on physiological state and nutritional factors are presented. The dependence between physiological state, type of nutrition, diet composition and total protein content and their saparate fractions interrelation, total lipids, triacylglicerols, nonesterificated fatty acids, glucose and carbamide level in cows blood has been shown. It has been also established that intensity of lipid and protein synthesis and energetically processes in skeletal muscles of cows at the beginning of lactation is lower than at the end particularly during interlactation period. The significant differences in utilisation of stearic and acetic acids, glucose and phenylalanine in substrate ensuring of lipid synthesis and energetically processes in skeletal muscles of cows at the beginning and at the end of lactation and also at the end of pregnancy have been established. It has been shown that increase of sugar and nondegraded protein part in cows diet influences enzymatic processes, bacterial protein synthesis in rumen, absorbtion and utilisation of milk precursors by mammary gland and increase of milk fat and lactose content. The increase of nondegraded protein part in diet of lactic cows causes decrease of cellulose digestion increase of protein digestion and utilisation, increase of blood glucose, acetic acid and ketone bodies concentration and their more effective utilisation in milk compounds synthesis in mammary gland. Key words: cows, nutrition, lactation, pregnancy, metabolic profile of blood, skeletal muscles, synthesis, protein, lipid, NI2.

Похожие записи