.

Вплив антибіотиків і середовищ для культивування ооцитів in vitro на якісні показники сперми бугаїв: Автореф. дис… канд. с.-г. наук / П.М. Скляров,

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OE?A?INUeEA AEAAeAI?ss AA?A?IEO IAOE

?INOEOOO OAA?EIIEOeOAA

Ia i?aaao ?oeiieno

Neey?ia Iaaei Ieeieaeiae/

OAeE 619.615.33:591.3591.463.1:636.22/.28

AIEEA AIOEA?IOEE?A ? NA?AAeIAEU AeEss

EOEUeOEAOAAIIss IIOeEO?A IN VITRO IA ssE?NI?

IIEACIEEE NIA?IE AOAA?A

Niaoe?aeuei?noue: 03.00.20. – a?ioaoiieia?y

Aaoi?aoa?ao

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe

Oa?e?a-1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia a eaai?aoi??? eoeueoeaoaaiiy ? o?ainieaioaoe?? aia??ii?a
?inoeoooo oaa?eiieoeoaa Oe?a?inueei? aeaaeai?? aa?a?ieo iaoe

Iaoeiaee ea??aiee aeieoi? a?ieia?/ieo iaoe, i?ioani?,

aieiaiee iaoeiaee ni?a?ia?oiee

a?aeae?eo a?ioaoiieia?? oa aaiii? ?iaeaia??? ?O OAAI

Aoa?ia Ieaen?e Aeieo?iae/

?inoeooo oaa?eiieoeoaa OAAI

Io?oe?ei? iiiiaioe:

1. Aeieoi? a?ieia?/ieo iaoe, i?ioani?, /eai-ei?aniiiaeaio OAAI,

Naoaoeueeee Ieeiea ?aaiiae/, aee?aeoi? ?inoeoooo ioao?aieoeoaa OAAI

2. Eaiaeeaeao a?ieia?/ieo iaoe, noa?oee iaoeiaee ni?a?ia?oiee,

E?oaeye Aiae??e Iao?iae/, caa?aeoaa/ eaai?aoi??? ?icaaaeaiiy
/a?aiii-?yai? ooaeiae oa aaiioiiaeo ii??ae ?inoeoooo ?icaaaeaiiy ?
aaiaoeee oaa?ei OAAI

I?ia?aeia onoaiiaa – Aea?aeaaiee iaoeiai-aeine?aeiee eiio?ieueiee
?inoeooo aaoa?eia?ieo i?aia?ao?a ? ei?iiaeo aeiaaaie, i. Euea?a

Caoeno aeena?oaoe?? a?aeaoaeaoueny 23 aa?aciy 1999 ?ieo i 1000 aiaeei?
ia can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae.65.356.02 i?e ?inoeooo?
oaa?eiieoeoaa OAAI (312120, Oa?e?anueea iaeanoue, Oa?e?anueeee ?aeii,
i/a Eoeeie/?)

C aeena?oaoe??th iiaeia iciaeiieoenue a a?ae?ioaoe? ?inoeoooo
oaa?eiieoeoaa OAAI

Aaoi?aoa?ao ?ic?neaiee 16 ethoiai 1999 ?ieo

A/aiee nae?aoa? niaoe?ae?ciaaii?

a/aii? ?aaee, eaiaeeaeao a?ieia?/ieo iaoe Ni?ao? O.I.

1

Caaaeueia oa?aeoa?enoeea ?iaioe

Aeooaeuei?noue oaie. Nia?ia aoaa?a aeei?enoiao?oueny aeey ooo/iiai
in?iai?iiy ? caie?aeiaiiy in vitro. A inoaiiueiio aeiaaeeo aeey ??
iiia?aaeiuei? ia?iaee (eaiaoeeoaoe??) ? eoeueoeaoaaiiy c iioeeoaie
canoiniaothoue ??ci? na?aaeiaeua ? aeiaaaee. Iaeiae, i?e oeueiio
i?aeoe/ii ia aeine?aeaeaiei caeeoa?oueny ieoaiiy i?i aieea oeeo
na?aaeiaeu oa aeiaaaie ia oaeee iieaciee nia?ie, ye aeaeeaai?noue.

I?e ooo/iiio in?iai?ii? aaaeeeaei ? oaea ieoaiiy, ye naiaoe?y nia?ie
aoaa?a-ie?aeiee?a. Aeooaeuei?noue eiai iia’ycaia c oei, ui ?nioth/?
iaoiaee iaea?aeaiiy oa caa??aaiiy nia?ie ia caaacia/othoue a on?o
aeiaaeeao a?eueii? a?ae i?e?ii?aai?ci?a nia?ie. Naiaoe?y i?e oeueiio ia
caaaeaee i?eaiaeeoue aei aaaeaieo ?acoeueoao?a a ca’yceo c oei, ui
aeaye? i?aia?aoe aai ia aoaeoeai? ii a?aeiioaiith aei i?e?ioei?e nia?ie,
aai ? oiene/ieie aei nia?i??a.

Iaoa aeine?aeaeaiue. Iaoith aeine?aeaeaiue aoei aea/aiiy aieeao ia
ye?ni? iieacieee nia?ie aoaa?a neioaoe/ieo na?aaeiaeu ? aeiaaaie, ye?
aeei?enoiaothoueny aeey eoeueoeaoaaiiy iioeeo?a in vitro, a oaeiae
?ic?iaea aoaeoeaiiai aioeaaeoa??aeueiiai i?aia?aoo aeey naiaoe?? nia?ie
aoaa?a-ie?aeiee?a.

A caaaeaiiy aeine?aeaeaiue aoiaeeei:

1. Anoaiiaeaiiy aieeao ia aeaeeaai?noue nia?i??a na?aaeiaeu ? aeiaaaie
aeey eoeueoeaoaaiiy iioeeo?a.

2.Aecia/aiiy aeaeeaaiino? nia?i??a a i?ioean? eaiaoeeoaoe??.

3. Aea/aiiy aieeao ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia
?acoeueoaoeai?noue e??ieiina?aaoe??.

4. Aecia/aiiy aieeao aaioai?oeeio oa aii?oeee?io ia aeaeeaai?noue
iaoeaii? nia?ie aoaa?a.

5. Aecia/aiiy aeaeeaaiino? ? caie?aeithaaeueii? caeaoiino? nia?i??a i?e
aeiaeaaaii? aioea?ioee?a ia?aae caii?iaeoaaiiyi ? i?e ?icii?iaeoaaii?.

6. ?ic?iaea aoaeoeaiiai aioei?e?iaiiai i?aia?aoo aeey naiaoe?? nia?ie
aoaa?a.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aia?oa aeaia a?ieia?/ia
ii??aiyeueia ioe?iea na?aaeiaeu ? aeiaaaie, ye? aeei?enoiaothoueny aeey
eoeueoeaoaaiiy iioeeo?a iica i?aai?ciii. Anoaiiaeaii iiceoeaiee aieea
na?aaeiaeu (TALP, BWW, A??inoa?a, ONI-199, E?aan-??iaa?a) ? aeiaaaie
(ai?oae?th iaoee ? yeoeaaiae?a, oie?eoey?ii? ??aeeie, oaoaeueii?
ne?iaaoee, aaia?eio, eioa?io), ye? aeei?enoiaothoueny aeey
eoeueoeaoaaiiy iioeeo?a ? aia??ii?a in vitro ia aeaeeaai?noue iaoeaii? ?
e??ieiina?aiaaii? nia?ie aoaa?a. Iaea?eueo aoaeoeaiei aoei na?aaeiaeua
TALP, a c aeiaaaie – ai?oae?e yeoeaaiaeo a eiioeaio?aoe?? 25%.
Aeieacaii, ui i?ioean ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia
/eieoue iaaaoeaiiai aieeao ia aeaeeaai?noue nia?i??a ia?aae ? i?ney
e??ieiina?aaoe??, a oaeiae ia ?o caie?aeithaaeueio caeaoi?noue.

Anoaiiaeaii iiceoeaiee aieea eiiieaeno aioea?ioee?a aaioai?oeeio ?
aii?oeee?io ia aeaeeaai?noue na?aei?icaaaeaii? ? caii?iaeaii-a?aeoaiooi?
nia?ie aoaa?a. Aecia/aii iioeiaeueio eiioeaio?aoe?th aaioai?oeeio – 25
iea/ie ? aii?oeee?io – 100 IAe, ye? cieaeothoue i?e?iaio eiioai?iaoe?th
nia?ie aoaa?a c? caa?aaeaiiyi ?? caie?aeithaaeueii? caeaoiino?.

?ic?iaeaii aioei?e?iaia na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie aoaa?a o
a?aee?eoeo a?aioeao ? niin?a o?eaaeiai eiai caa??aaiiy a ??aeeiio acio?.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a. I?iaaaeaii ii??aiyeueia
aea/aiiy aeaeeaaiino? nia?i??a aoaa?a i?e aeei?enoaii? na?aaeiaeu ?
aeiaaaie aeey eoeueoeaoaaiiy in vitro, a oaeiae aea/aii aeaeeaai?noue
nia?i??a o i?ioean? ia?iaee ?o ia?aae caie?aeiaiiyi in vitro ? aecia/aii
aieea oeueiai i?ioeano ia ?acoeueoaoeai?noue e??ieiina?aaoe??.

?ic?iaeaii i?aia?ao aeey naiaoe?? nia?ie aoaa?a, yeee caaacia/o?
i?aeaeuaiiy caie?aeiaiino? nia?ie ia 11-18 % ? ??aaiue i?e?iaii?
caa?oaeiaiino? o a?aeiia?aeiino? aei aeiia aea?aenoaiaea?oo aeey nia?ie,
caii?iaeaii? o a?aee?eoeo a?aioeao. E??i oeueiai, i?aeoe/ia cia/aiiy
i?aia?aoo iieyaa? a oiio, ui a?i ia? aioei?e?iaio aeoeai?noue i?ioyaii
o?eaaeiai /ano i?e caa??aaii? a ??aeeiio acio? ? caaacia/o? naiaoe?th
nia?ie aaciina?aaeiuei ia?aae aaaaeaiiyi ?? a noaoaa? oeyoe naiee.

Iniaenoee aianie caeiaoaa/a. A?eueo?noue aeine?aeaeaiue aeeiiaii aaoi?ii
naiino?eii. Iaoeiai-ae?iaie/? aeine?aee i?iaaaeaii ca o/anoth aaoi?a.
Iaoiaee/i? i?aeoiaee oa noaie aeine?ae?a a?aei?aoeueiaaii ni?eueii c
iaoeiaei ea??aieeii.

Ai?iaaaeaeaiiy ?acoeueoao?a aeine?aeaeaiue. ?acoeueoaoe aeine?aeaeaiue
ai?iaaaeaeaii a ae/a “Eoooc?aea”, a?aeae?eeo “O?oica” ae/a “Oe?a?iea” oa
aea?aeieaicaaiae? “*a?aiiee Aaeaoaiue” Oa?e?anueei? iaeano?.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??. ?acoeueoaoe aeine?aeaeaiue aoee
aeeeaaeai?, iaaiai?ai? ? io?eiaee iiceoeaio ioe?ieo ia a/aieo ?aaeao
?inoeoooo oaa?eiieoeoaa OAAI, a oaeiae ia I?aeia?iaei?e
iaoeiai-i?aeoe/i?e eiioa?aioe??, i?enay/ai?e 100-??//th a?ae aeiy
ia?iaeaeaiiy ssoeaiea I.A. (Oa?e?a, 1997?.); I?aeia?iaei?e
iaoeiai-ae?iaie/?e eiioa?aioe?? “Aeei?enoaiiy o?ainieaioaoe?? aia??ii?a
a naeaeoe?? ? a?aeoai?aii? n?euenueeiainiiaea?nueeeo oaa?ei”
(Aneai?y-Iiaa, 1997 ?.); I?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe??,
i?enay/ai?e 100-??//th a?ae aeiy ia?iaeaeaiiy N.C. Aaeeoeueeiai “No/ani?
i?iaeaie a?ieia??, aaoa?eia?ii? iaaeeoeeie, cii?iaeaia??? oa oaoiieia??
i?iaeoeo?a oaa?eiieoeoaa” (Euea?a, 1997?.); I?aeia?iaei?e eiioa?aioe??,
i?enay/ai?e 70-??//th a?ae aeiy ia?iaeaeaiiy O.?. Inoaoei (Oa?e?a,
1998?.); eiioa?aioe?? iieiaeeo a/aieo ?O OAAI (Oa?e?a, 1998 ?.);
I?aeia?iaei?e eiioa?aioe?? “Aeei?enoaiiy no/anieo iieaeoey?ii-aaiaoe/ieo
? a?ioaoiieia?/ieo ?ic?iaie o aaiaoeei-naeaeoe?eieo aeine?aeaeaiiyo”
(Iaeana, 1998 ?.); I?aeia?iaei?e iaoeia?e eiioa?aioe?? “No?aoaaey e
oaeoeea ai?ueau n eioaeoeeiiiuie aieaciyie” (Oa?e?a, 1998 ?.).

Ianya oa no?oeoo?a aeena?oaoe??. Aeena?oaoe?y aeeeaaeaia ia 186
noi??ieao iaoeiiieniiai oaenoo ? neeaaea?oueny c anooio, iaeyaeo
e?oa?aoo?e, iaoa??ae?a oa iaoiae?a aeine?aeaeaiue, aeniiae?a oa
i?iiiceoe?e ae?iaieoeoao. I?noeoue 38 oaaeeoeue, 1 ?enoiie oa 11
aeiaeaoe?a. Nienie e?oa?aoo?e aeeth/a? 352 iaeiaioaaiiy, o oiio /ene?
179 ?iicaiiith iiaith.

Iaoa??aee oa iaoiaeeea aeine?aeaeaiue

Eaai?aoi?i? aeine?aeaeaiiy i?iaiaeeeeny a a?aeae?e? a?ioaoiieia?? ?
aaiii? ?iaeaia??? ?inoeoooo oaa?eiieoeoaa OAAI ? Oa?e?anuee?e
iaenaiai?aenoaioe??; iaoeiai-ae?iaie/? aeine?aee – a a?aeae?eeo “O?oica”
ae/a “Oe?a?iea”, ae/a “Eoooc?aea” ? AeIC “*a?aiiee Aaeaoaiue”
Oa?e?anueei? iae.

Iaoa??aeii aeey aeine?aeaeaiue aoea iaoeaia nia?ia, iaea?aeaia a?ae
aoaa?a-ie?aeiee?a, ye? oo?eioaaeeny ia noaioe?? ooo/iiai in?iai?iiy ae/a
“Oe?a?iea”, ? caii?iaeaii-a?aeoaiooa nia?ia c e??iaaieo a?aeae?eo
a?ioaoiieia?? ? aaiii? ?iaeaia???.

O aeine?aeao aeei?enoiaoaaee na?aaeiaeua TALP, BWW, A??inoa?a, ONI-199,
E?aan-??iaa?a ? aeiaaaee – aaia?ei, eioa?i, ai?oae?e iaoee ? yeoeaaiaeo,
oie?eoey?io ??aeeio, oaoaeueio ne?iaaoeo.

Eaiaoeeoaoe?th nia?i??a i?iaiaeeee oeyoii ?ieoaaoe?? c aaia?eiii oa
oeaio?eooaoaaiiy ca iaoiaeeeith J.J. Parrish et al. (1986).

Caii?iaeoaaiiy nia?ie a iaeeoeueiaaieo oa a?aee?eoeo a?aioeao i?iaiaeeee
ca Oa?e?anueeith oaoiieia??th ? ia ooi?iieanoia?e ieanoei? ca
caaaeueiii?eeiyoith iaoiaeeeith c ?ic?aooieo ia iaio 15 iei. aeoeaieo
nia?i??a a aeic? i?ney ?icii?iaeoaaiiy.

Na?aaeiaeua c aioea?ioeeaie aiooaaee c ?ic?aooieo 12, 25, 50 /e 100 iea
aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie na?aaeiaeua, aeey /iai
aeei?enoiaoaaee 4%-iee ?ic/ei aaioai?oeeio noeueoaoo oa aii?oeee?io
iao???ao n?eue. Nia?iinai-3 aeiaeaaaee c ?ic?aooieo 50 oen. IAe ia 100
ie na?aaeiaeua.

Naioth/a na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie i?ney i?eaiooaaiiy
?icoaniaoaaee a iie?aoeeaiia? aiioee ii 1 ie, aa?iaoe/ii oiaeiaoaaee,
caii?iaeoaaee oeyoii caio?aiiy a ??aeeee acio, a yeiio caa??aaee aei
aeei?enoaiiy. I?e aeaeiina?aaoe?? nia?ie iiia?aaeiuei ?icii?iaeoaaee
na?aaeiaeua ia aiaeyi?e aai? i?e t=40°N i?ioyaii 5 oa., a iio?i
aeei?enoiaoaaee eiai ye cae/aeiee 2,9 %-iee ?ic/ei oeeo?aoo iao??th.

Aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? nia?ie aecia/aee ca
A.E. I?eiaaiiaei (1962):

picscalex122010009000003730100000300170000000000050000000902000000000400
000002010100050000000102ffffff00040000002e011800050000003102010000000500
00000b0200000000050000000c02e003000a1200000026060f001a00ffffffff00001000
0000c0ffffffbeffffffc00900009e0300000b00000026060f000c004d61746854797065
0000c00009000000fa02000010000000000000002200040000002d010000050000001402
000239030500000013020002030510000000fb02c0fd0000000000009001000000020002
001053796d626f6c0002040000002d01010008000000320ab902b00601000000e5001000
0000fb0280fe0000000000009001000000020002001053796d626f6c0002040000002d01
020004000000f001010008000000320a60029d05010000002b0008000000320a6002fc01
010000003d0017000000fb0280fe0000000000009001010000cc0402001054696d657320
4e657720526f6d616e2043797200cc7e040000002d01010004000000f001020008000000
320a6002860802000000617408000000320a6e01530301000000610008000000320a6002
340001000000530017000000fb0220ff0000000000009001010000cc0402001054696d65
73204e657720526f6d616e2043797200cc7e040000002d01020004000000f00101000800
0000320ac002210101000000610017000000fb0280fe0000000000009001000000cc0402
001054696d6573204e657720526f6d616e2043797200cc7e040000002d01010004000000
f001020008000000320a8a03be0301000000320017000000fb0220ff0000000000009001
000000cc0402001054696d6573204e657720526f6d616e2043797200cc7e040000002d01
020004000000f001010008000000320ace0167040100000031000a00000026060f000a00
ffffffff01000000000010000000fb021000070000000000bc02000000cc010202225379
7374656d00cc040000002d01010004000000f0010200030000000000,

aea
picscalex116010009000003b60000000200170000000000050000000902000000000400
000002010100050000000102ffffff00040000002e011800050000003102010000000500
00000b0200000000050000000c02800240021200000026060f001a00ffffffff00001000
0000c0ffffffa6ffffff00020000260200000b00000026060f000c004d61746854797065
0000800017000000fb0220ff0000000000009001000000cc0402001054696d6573204e65
7720526f6d616e2043797200ccdc040000002d01000008000000320ae001410101000000
310017000000fb0280fe0000000000009001010000cc0402001054696d6573204e657720
526f6d616e2043797200ccdc040000002d01010004000000f001000008000000320a8001
3a000100000061000a00000026060f000a00ffffffff01000000000010000000fb021000
070000000000bc02000000cc0102022253797374656d00cc040000002d01000004000000
f0010100030000000000 – iieaciee ii/aoeiai? aeoeaiino? nia?i??a;
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- noia
iieaciee?a aeoeaiino? /a?ac eiaeio aiaeeio.

Eiao?oe??io oiene/iino? (iaoe?aeeeaino?) ?ic?aoiaoaaee ca oi?ioeith,
cai?iiiiiaaiith I.A. Aaeaoiaei (1972):
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aea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 – na?aaei? /enei ?ooeeaeo nia?i??a o aeo?aei?e
nia?i?;
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 – na?aaei? /enei ?ooeeaeo nia?i??a /a?ac 3 aiaeeie
i?ney ?icaaaeaiiy nia?ie na?aaeiaeuai c i?aia?aoii.

Caie?aeithth/o caeaoi?noue nia?i??a aecia/aee ca a?aenooiino? ianooiieo
noaoaaeo oeeee?a i?ioyaii 2-o i?nyoe?a ? ca aeaieie ?aeoaeueiiai
aeine?aeaeaiiy ia o?euei?noue i?ney in?iai?iiy /a?ac 3 i?nyoe?.
Caie?aeiai?noue aecia/aee ca a?aenioeii o?eueieo a?ae caaaeueii?
e?eueeino? ia?aeiii in?iaiaieo ei??a. A aeine?ae? ? eiio?ie?
aeei?enoiaoaaee ee?i?/ii caei?iaeo oaa?ei ca i?eioeeiii a?oi-aiaeia?a ca
ii?iaeith, a?eii ? /anii i?iyao ia?oiai noaoaaiai oeeeeo i?ney ioaeaiiy.
Aeyaeaiiy ei??a a ioio? i?iaiaeeee aea?/? ia aeiao (a?aioe? oa aaa/a??).
Nia?io aaiaeeee iaii- ? ?aeoi-oea?a?eaeueiei niiniaaie ca?aeii ae?th/i?
?ino?oeoe??.

A?aea?? i?ia oa aaeoa??ieia?/i? aeine?aeaeaiiy i?iaiaeeee a?aeiia?aeii
aei aea?aenoaiaea?o?a 20909.1-75 oa 20909.2-75.

Noaoenoe/io ia?iaeo ?acoeueoao?a i?iaiaeeee ca aeiiiiiaith
ia?ni-iaeueiiai eiii’thoa?a Pentium-120 ca?aeii i?ia?aiiiai
caaacia/aiiy: WORD 7.0, EXEL 7.0, MICROSOFT OFFICE o??ie “MICROSOFT”
(Ieaei E.A., Ieioeineee I.A., 1970, Eaeei A.O., 1990).

?acoeueoaoe aeine?aeaeaiue

A ia?o?e na??? aeine?ae?a ie aea/aee aieea ia aeaeeaai?noue nia?i??a
na?aaeiaeu oa aeiaaaie aeey eoeueoeaoaaiiy in vitro iioeeo?a.
?acoeueoaoe aeine?aeaeaiue i?eaaaeaii a oaae. 1.

Oaaeeoey 1

Aeaeeaai?noue nia?i??a a na?aaeiaeuao aeey eoeueoeaoaaiiy in vitro

Na?aaeiaeuaIaoeaia nia?iaCaii?iaeaii-a?aeoaiooa
nia?iaeiio?ieueaeine?aeeiio?ieueaeine?ae1.TALP:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,3±0,40

42,3±2,1

12

11,4±0,26 **

49,8±2,2 *

12

6,1±0,24

19,9±0,9

12

8,1±0,24 **

23,5±1,0

122. BWW:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,8±0,22

43,6±1,3

15

10,5±0,30 **

46,4±1,3

15

6,1±0,27

20,8±0,9

10

7,7±0,15 **

24,1±0,7

103. A??inoa?a:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

9,1±0,23

43,2±1,4

12

10,8±0,27 **

46,3±1,2

12

6,1±0,24

18,9±0,5

11

6,6±0,26 *

21,2±0,6

114. ON-199:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

9,9±0,22

47,1±1,1

11

11,0±0,36

50,8±1,4

11

5,9±0,26

26,1±1,3

9

7,1±0,26 *

29,3±1,2

95. E?aan-??iaa?a:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,3±0,35

43,2±1,6

12

8,8±0,36

44,4±1,5

12

6,2±0,24

24,4±0,5

12

6,5±0,26

24,9±0,6

12* – P

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