OA?EIANUeEEE AeA?AEAAIEE OIIAA?NEOAO

AeCTHAA AIEOI?Iss IEEIEA?AIA

OAeE 57.017.67:577.352.38

AIEIAI INIAEEAINOI AIEEAO IOAII? IA

NO?OEOO?II-OOIEOeIIIAEUeIEE NOAI IIOIOIIAe?IE

I IA?AEENIA IEENEAIIss EIIIAeIA NA?Oess UO?IA

03.00.04 — aiioiiiy

AAOI?AOA?AO

aeena?oaoei? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa aiieiai/ieo iaoe

Oa?eia — 1998

?iaioa aeeiiaia a iaoeiai-aeineiaeiiio iinoeoooi aiieiai? Oa?eianueeiai
aea?aeaaiiai oiiaa?neoaoo Iiiinoa?noaa inaioe Oe?a?ie

Iaoeiaee ea?iaiee: aeieoi? aiieiai/ieo iaoe, i?ioani?

Eaiaoei Aieoi? Aaneeueiae/, aee?aeoi? iaoeiai-

aeineiaeiiai iinoeoooo aiieiai? Oa?eianueeiai

aea?aeaaiiai oiiaa?neoaoo Iiiinoa?noaa inaioe Oe?a?ie

Ioioeieii iiiiaioe: aeieoi? aiieiai/ieo iaoe, i?ioani?

Iao?aiei Ieaenaiae? TH?ieiae/, i?iaiaeiee iaoeiaee

niia?iaioiee aiaeaeieo aenia?eiaioaeueii? e?iiiaaee-

oeeie iinoeoooo i?iaeai e?iiaiieiai? i e?iiiaaeeoeeie

IAI Oe?a?ie

aeieoi? aiieiai/ieo iaoe, i?ioani?

AEaaoiia Aaiaaeie Oaaei?iae/, caaiaeoaa/ eaoaae?ith

iaaee/ii? aiieiai?, ia?aceoieiai? oa aaiaoeee

Oa?eianueeiai aea?aeaaiiai iaaee/iiai oiiaa?neoaoo

Iiiinoa?noaa ioi?iie caei?ia’y Oe?a?ie

I?iaiaeia onoaiiaa: Iinoeooo aa?iioieiai? (eaai?aoi?iy iieaeoey?ii?

aaiaoeee) AII Oe?a?ie, i.Ee?a.

Caoeno aiaeaoaeaoueny «16» a?oaeiy 1998 ?. i 1515 aiaeeii

ia caniaeaiii niaoeiaeiciaaii? a/aii? ?aaee E 64.051.17 i?e
Oa?eianueeiio aea?aeaaiiio oiiaa?neoaoi Iiiinoa?noaa inaioe Oe?a?ie ca
aae?anith:

310077, i. Oa?eia, i. Naiaiaee, 4, aoae. III-15.

C aeena?oaoei?th iiaeia iciaeiieoeny o Oeaio?aeueiie iaoeiaie
aiaeiioaoei

Oa?eianueeiai aea?aeaaiiai oiiaa?neoaoo Iiiinoa?noaa inaioe Oe?a?ie ca
aae?anith: 310077, i. Oa?eia, i. Naiaiaee, 4.

Aaoi?aoa?ao ?icineaiee «13» eenoiiaaea 1998 ?.

A/aiee nae?aoa?

niaoeiaeiciaaii? a/aii? ?aaee

eaiaeeaeao aiieiai/ieo iaoe,

noa?oee iaoeiaee niia?iaioiee
Iaaeaeei A.I. 1

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeueiinoue oaie. Na?oeaai-noaeeiii caoai?thaaiiy ciaoiaeyoueny ia
iaeiiio c ia?oeo iinoeue a no?oeoo?i caoai?thaaiiy oa nia?oiinoi
iana-eaiiy a aieueoinoi e?a?i naioo [Eii ?.I., 1992]. Oeae oaeo ii?yae c
ii-oe?aiiyi iia?aoeieieo ao?o/aiue, yei iio?aaothoue oei/aniaiai
i?eieiaiiy e?iaiiaiao a i?aaiao, a oaeiae ona aieueo /anoa aeei?enoaiiy
o?ain-ieaioaoei? i?aaiia, ?iaeyoue ?icoiiiiy iieaeoey?ieo iaoaiiciia
ioaii/iiai iioeiaeaeaiiy i?aaiia iaeii?th c iaeaeooaeueiioeo i?iaeai
no-/anii? iaoee. Aeooaeueiinoue i?iaeaie oaeiae coiiaeth?oueny
iaiaoiaeiinoth ?ic?iaee iiaeo caniaia caiiaiaaiiy ioaii/iiai
iioeiaeaeaiiy oeaiei.

A eioa?aoo?i ? aeinoaoiuei aaeeea eieueeinoue aeaieo aiaeiinii aieeao
ioaii? ia no?oeoo?ii-ooieoeiiiaeueiee noai iiiea?aeo [Eaiaoei A.A. e
ae?., 1986; Aeeaiei I.A., 1989; Kleber A.G., 1990; Downey J.M., 1990;
Coudray C. et al., 1995; Tani M. et al., 1997]. Iacaaaeath/e ia oea,
iaoaiicie ioaii/iiai iioeiaeaeaiiy, i?e/eiii-ianeiaeiee ca’ycie iiae
aa-aaoueia iaoiaaiaoe/ieie oaeoi?aie oa aieiai iniaeeainoi ioaii/iiai
iioeiaeaeaiiy i?aaiia i oeaiei caeeoathoueny ia nueiaiaeiioiie aeaiue
iaaein-oaoiuei aea/aieie.

Iaeio c aieiaieo ?ieae o iaoaiicii ioaii/iiai oa ?aia?oocieiiai
iioeiaeaeaiiy oeaiei aieueoinoue aeineiaeieeia i?eaeieythoue
aieueii?aaeeeaeue-iiio ia?aeeniiio ieeneaiith eiiiaeia (IIE), oi/a aeaii
aiaeiinii iioai-neaiinoi IIE i noaio oa?iaioaoeaii? aioeieneaeaioii?
nenoaie na?oey i?e ioaii? aeinoaoiuei noia?a/ii. Cie?aia, iniothoue
aeaii, ui naiae/aoue, ye i?i iineeaio aaia?aoeith aieueieo
?aaeeeaeia [Suri R.K. et al., 1996] i cieaeaiiy aeoeaiinoi
aioeieneaeaioieo oa?iaioia [Simmons T.W. et al., 1989; Porreca E.
et al., 1994] i?e ioaii?, oae i i?i aiaenooiinoue ciii
ieenethaaeueiiai oa aioeieneaeaioiiai noaoono na?oey [Ji L.L. et
al., 1994; Coudray C. et al., 1995] i?e oeueiio aeaei iaoi-eiai?.

A eioa?aoo?i iaia? oaeiae /ioei? aiaeiiaiaei ia ieoaiiy i?i ia?oi-?yaeia
cia/aiiy iioeiaeaeaiiy ?icieo noaeeioeiieo no?oeoo? a ioaii/iiio
ii?ooaiii ooieoeie eeioei i oeaiei [Hoffstein S. et al., 1975; Nayler
W.G., 1982; Aeaio?ia A.A. e ae?., 1985]. Aaeeeo oaaao a oeueiio ieaii
i?eaa?oathoue aei naaa iioioiiae?i? [Schmiedl A. et al., 1990;
Veitch K. et al., 1992; Miller T.W. et al., 1995], ineieueee aiie ?
iniiaiei niiaeeaa/ai eenith oa iaeii/anii aieiaiei aeaea?aeii aia?ai? a
eeioeii.

Aeoiaey/e ci neacaiiai aeua oa i?eeiath/e aei oaaae ?aiio i?iaaaea-

2

ii aeineiaeaeaiiy a iaoie oa iioeo eaai?aoi?iyo [Eaiaoei A.A. e ae?.,
1989; Stio M. et al., 1994] aiaeiinii aieeao noa?iiiy oaa?ei ia
?iaaiue aia?aaoe/iiai iaiiio oa eiiiia?aeeniee iioaioeiae iiiea?aeo,
iai oyaeyeiny aaaeeeaei i?iaaaeaiiy ii?iaiyeueiiai aiaeico ciii
iioai-neaiinoi IIE, eiai oa?iaioaoeaii? ?aaoeyoei? oa
no?oeoo?-ii-ooieoeiiiaeueiiai noaio iioioiiae?ie iiiea?aeo iieiaeeo i
noa?eo uo?ia i?e ioaii?.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. Aeena?-oaoeieia
?iaioa aeeiiaia caiaeii c ieaiaie iaoeiai-aeineiaeieo ?iaio: «Onoaiiaeoe
?ieue oa?iaioaoeaii? ?aaoeyoei? ia?aeeniiai ieeneaiiy eiiiaeia a
iaoaiiciao aieiai? ia?aaoaeiae oa ioaii/iiai iioeiaeaeaiiy iaia?ai
iiiea?aeo oa iioeo oeaiei» (N aea?ae. ?a?no?aoei? 0187.0009268, Oa?eia:
1990), «Aea/aiiy aieiaeo iniaeeainoae ioaii/iiai iioeiaeaeaiiy oeaiei i
?ic?iaea e?eoa?i?a iiooeo iiaeo ea?aeii- i aa?ii?ioaeoi?ia» (N aea?ae.
?a?no?aoei? 01.9.10036893, Oa?eia: 1993), «Aeineiaeaeaiiy
eiii-ia?aeeniiai iaoaiicio ioaii/iiai iioeiaeaeaiiy aiiiaia?ai i
?ic?iaea iiaeo iaia?aiii?ioaeoi?ia» (N aea?ae. ?a?no?aoei? UA 01008697P,
Oa?eia: 1996).

Iaoa i caaea/i aeineiaeaeaiiy. Iaoith ?iaioe aoei c’ynoaaiiy aieiaeo
caeiiiii?iinoae ciiie no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie,
iioaineaiinoi IIE i eiai ?aaoeyoei? i?e oioaeueiie ioaii? oa ioaii?
-?aia?ooci? na?oey uo?ia.

Aeoiaey/e c iaoe, aoee aecia/aii ianooiii caaea/i:

1. Aea/eoe aieiai iniaeeainoi aieeao oioaeueii? oa ia?a?aaii? ioaii?
na?oey uo?ia ia iioaineaiinoue IIE.

2. Aeineiaeeoe aieiai iniaeeainoi aieeao ioaii? ia noai oa?iaioaoeaii?
aioeieneaeaioii? nenoaie iiiea?aeo uo?ia.

3. Aea/eoe aieiai iniaeeainoi aieeao oioaeueii? ioaii? ia
no?oeoo?-ii-ooieoeiiiaeueiee noai iioioiiae?ie na?oey.

4. Ioeiieoe i?ioeioaii/io aeith ?yaeo i?e?iaeieo iaoaaieioia c iaoith
?ic-?iaee caniao caoenoo iiiea?aeo aiae ioaii/iiai iioeiaeaeaiiy.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoaoia. Aia?oa aeineiaeaeaii aieiai
ciiie iioaineaiinoi IIE oa eiai ?aaoeyoei?, iioeiaeaeaiiy na?eieaie oa
ii?ooaiiy no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie o aeeiaiioei
ioaii/iiai iioeiaeaeaiiy iiiea?aeo uo?ia i anoaiiaeaii, ui aeoeaaoeiy
IIE aiaeaoaa?oueny ia?aae ii?ooaiiyi oeieinoiinoi na?eieaie oa no?oeoo?e
i ooieoei? iioioiiae?ie i ? neeaaeiaith /anoeiith iaoaiicio
iioeiaeaeaiiy iaia?ai ea?aeiiiiioeeoia. Aeyaeaii, ui iaeaieueoo
/ooeeainoue aei

3

ioaii/iiai iioeiaeaeaiiy aeyaey? iiiea?ae noa?eo uo?ia.

Ia iiaenoaai aeineiaeaeaiiy i?ioeioaii/ieo aeanoeainoae ?yaeo
i?e?iae-ieo iaoaaieioia ?ic?iaeaii na?aaeiaeua aeey caiiaiaaiiy
ioaii/iiai iioeiaeaeaiiy oeaiei iiae /an o?ainieaioaoei? i?aaiia, yea
nooo?ai ciai-oo? iioeiaeaeaiiy iaia?ai ea?aeiiiiioeeoia, iaeiie/aiiy
i?iaeoeoia IIE oa iieiioo? aiaeiiaeaiiy nei?i/oaaeueii? aeoeaiinoi
na?oey iiney ioaii? — ?aia?ooci? (A.n. SU 1632427 A1, A 61 E 9/08).

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoaoia. ?acoeueoaoe i?iaaaeaieo
aeineiaeaeaiue aeicaieythoue aeeaaea c?icoiioe i?e/eiii-ianeiaeiee
ca’ycie iiae ?icieie iaoiaaiaoe/ieie oaeoi?aie, yei i?ecaiaeyoue aei
iacai?io-iueiai iioeiaeaeaiiy na?oey i?e ioaii?, i iioe?eoe inioth/i
oyaeaiiy i?i iaoaiicie ?aaeoei? eeioei iiiea?aeo ia ioaii/iee aieea.

Ia iniiai iaea?aeaieo aeaieo aiaeiinii i?ioeioaii/ieo aeanoeainoae
i?e?iaeieo iaoaaieioia ?ic?iaeaii na?aaeiaeua aeey iiia?aaeaeaiiy
ioaii/iiai iioeiaeaeaiiy i?aaiia, yea iiaea aooe aeei?enoiaaiei a
aen-ia?eiaioaeueiie i eeiii/iie ea?aeiieiai? (A.n. SU 1632427 A1, A 61 E
9/08).

Iniaenoee aianie caeiaoaa/a. I?e aeeiiaiii aeena?oaoeieii? ?iaioe aaoi?
iniaenoi aea?aa oa e?eoe/ii ioeiiea i?enooiio eioa?aoo?o ca oa-iith
aeineiaeaeaiiy. Iiae /an aeeiiaiiy aenia?eiaioaeueii? /anoeie ?iaioe
aeineiaeaeaiiy noiniaii iioaineaiinoi i?ioeania IIE oa
no?oeoo?-ii-ooieoeiiiaeueiiai noaio iioioiiae?ie /anoeiai aoee
i?iaaaeaii niieueii ci niia?iaioieeaie IAeI aiieiai? OAeO e.a.i.
Iieio/aiei TH.A. oa e.a.i. Oaeiaieiaith I.A., ui ciaeoei aiaeia?aaeaiiy
o niieueieo ioaeieaoeiyo. Aeianaii ia caoeno iieiaeaiiy i ?acoeueoaoe
iaea?aeaii aee-na?oaioii naiinoieii. Aeena?oaioii ?acii c iaoeiaei
ea?iaieeii i?iaa-aeaii aiaeic iaea?aeaieo ?acoeueoaoia i c?iaeaii
aeniiaee ii ?iaioi.

Ai?iaaoeiy ?acoeueoaoia aeena?oaoei?. Iniiaii iieiaeaiiy ?iaioe
aei-iiaiaeaeeny ia II iaoeiiiaeueiiio eiia?ani aa?iioieiaia i aa?iao?ia
Oe-?a?ie (i.Ee?a, 1994 ?.), ia iaoeiai-i?aeoe/iie eiioa?aioei? «Iaoeiai
aeinyaiaiiy i i?iaeaie ae?iaieoeoaa eiea?nueeeo caniaia» (i.Oa?eia,
1995 ?.), ia eiioa?aioei? iieiaeeo o/aieo aiieiai/iiai oaeoeueoaoo i
IAeI aiieiai? (i.Oa?eia, 1997 ?.), ia III iiaeia?iaeiiio niiiicioii
«Aiieiai/ii iaoaiicie noa?iiiy» (i.Oa?eia, 1998 ?.).

Ioaeieaoei?. Iniiaii iieiaeaiiy ?iaioe iioaeieiaaii o 17 iaoeiaeo
?iaioao, na?aae yeeo 4 noaooi oa 1 aaoi?nueea naiaeioeoai.

No?oeoo?a oa ianya aeena?oaoei?. Aeena?oaoeiy neeaaea?oueny ic anooio,
iaeyaeo eioa?aoo?e c i?iaeaie, 3 ?icaeieia, ui aiaeia?aaeothoue
?acoeueoaoe

4

aeanieo aeineiaeaeaiue, aeniiaeia oa nieneo aeei?enoaieo aeaea?ae, yeee
iinoeoue 239 aeaea?ae. ?iaioo aeeeaaeaii ia 128 noi?iieao iaoeiiieno,
iethno?iaaii 11 ?enoieaie i 21 oaaeeoeath.

IAOA?IAEE I IAOIAeE AeINEIAeAEAIIss

O aeineiaeao aeei?enoiaoaaee uo?ia eiii? Ainoa? 1-, 3-, 12- i
24-iiny/iiai aieo.

Ioaii/ia iioeiaeaeaiiy eeioei na?oey aea/aee ia aeaio iiaeaeyo:
oi-oaeueii? ioaii? i ia?a?aaii? ioaii? icieueiaaiiai na?oey,
ia?oociaaiiai na?aaeiaeuai Oaiena aac aetheice ca noaiith «19 oaeeei
ioaii? — 1 oae-eeia ?aia?ooci?». I?e aea/aiii i?ioeioaii/ieo
aeanoeainoae i?e?iaeieo iaoaaieioia aeineiaeaeoaaio ?a/iaeio
aeiaeaaaee o aecia/aiie eiioeaio?aoei? a na?aaeiaeua ia?ooci? na?oey.

Aiaeic iioeiaeaeaiiy iioioiiae?ie na?oey i?iaiaeeee a nenoaii
aiiiaa-iaoo oeaieie. Oaeaeeinoue aeeoaiiy i ieenethaaeueiiai
oinoi?eethaaiiy aec-ia/aee iiey?ia?aoi/iei iaoiaeii [Eiiae?aoaaa I.I. e
ae?., 1973]. Ii e?eaeo niiaeeaaiiy eenith ?ic?aoiaoaaee oaeaeeinoue
aeeoaiiy a iaoa-aiei/ieo noaiao 3 i 4 ca *ainii (V3 i V4), a
?ic’?aeiaiiio noaii (V3?) i aeeoaeueiee eiio?ieue (AeE). AOOacio
aeoeaiinoue aiiiaaiaoia na?-oey aeii?thaaee iioaioeiiiao?e/iei iaoiaeii
[Aieaeu?aa A.A., 1977]. Aeni-eiaiieiooaeia iaaooaiiy iioioiiae?ie
?a?no?oaaee ii ciiiaiith iioe/ii? uieueiinoi i?e aeiaaeeii oaeei 610 ii
[Oaeiaeeiaa A.A., 1994].

Iioaineaiinoue ia?aeeniiai ieeneaiiy eiiiaeia ioeiithaaee ii
iioai-neaiinoi oaiiethiiianoeaioei? (OEI) i oaeaeeinoi iaeiie/aiiy
iaeiiiaiai aeiaeueaeaaiaeo (IAeA) ye iienaii a ?iaioi [Eaiaoei A.A. e
ae?., 1987]. Aiino aiae?iia?aeenia eiiiaeia o aiiiaaiaoao na?oey
aecia/aee ca iaoi-aeii Ohkawa et al. c aeayeeie iiaeeoieaoeiyie
[Eaiaoei A.A. e ae?., 1986].

Aeooaoiiiia?ieneaeacio aeoeaiinoue aeii?thaaee niaeo?ioioiiao?e/ii a
ni?yaeaiie aeooaoiii?aaeoeoaciie ?aaeoei? ca iaoiaeii Paglia et al. c
aeayeeie iiaeeoieaoeiyie [Eaiaoei A.A. e ae?., 1987].
Aeooaoiii-S-o?ain-oa?acio aeoeaiinoue aeii?thaaee niaeo?ioioiiao?e/ii
i?e 340 ii [Jones M. et al., 1980] c 1-oei?-2,4-aeiiio?iaaicieii a
yeinoi noano?aoo. Aeooaoiii?aaeoeoacio aeoeaiinoue aecia/aee
oeoi?eiao?e/ii ca ciaioai-iyi ?iaith NADPH [Aa?aneiia A.I. e ae?.,
1976]. Eaeoaoaeaaiae?iaaiacio aeoeaiinoue a ia?oocaoao ioaiiciaaieo i
eiio?ieueieo na?aeaoeue aeii?thaa-ee ca iaoiaeii [Eaaiia E.E. e ae?.,
1974], ?a?no?oth/e oeoi?eiao?e/ii

5

aiaeiiaeaiiy NAD+. Aetheici-6-oinoaoaeaaiae?iaaiacio aeoeaiinoue
aeii?th-aaee oeoi?eiao?e/ii ii oaeaeeinoi aiaeiiaeaiiy NADP+ [Baquer
N.Z. et al., 1967]. NADP+-caeaaeio iaeaoaeaaiae?iaaiacio aeoeaiinoue
aecia/aee oeoi?eiao?e/ii ii oaeaeeinoi aiaeiiaeaiiy NADP+ [Onaoaiei I.N.
e ae?., 1974]. NADP+-caeaaeio icioeeo?aoaeaaiae?iaaiacio aeoeaiinoue
aecia/aee oeoi?eiao?e/ii ii oaeaeeinoi aiaeiiaeaiiy NADP+ [Bauman D.E.
et al., 1970]. Noia?ieneaeaeeniooacio (NIAe) aeoeaiinoue aecia/aee
niaeo?ioioi-iao?e/ii ca iaoiaeii Fridovich o iiaeeoieaoei? Eaieiia
[Eaieei A.C. e ae?., 1976]. Eaoaeacio aeoeaiinoue aecia/aee
niaeo?ioioiiao?e/ii i?e 240 ii [Marklund S. et al., 1981]. ?iaaiue
iai?aaii/iiai oinoaoo (Oi) aecia/aee ye iienaii [?uaaeue/aiei A.E. e
ae?., 1988].

Aeno?aaoaaiiy eiiiaeia ic iaia?ai iiiea?aeo i?iaiaeeee ye iienaii [Folch
J. et al., 1957]. Aiino caaaeueieo eiiiaeia aecia/aee ca
iaoi-aeii [March J.B. et al., 1966]. Aioeieenethaaeueio aeoeaiinoue
aeno?aai-aaieo eiiiaeia aecia/aee ye iienaii a ?iaioi [Eeaaaiia A.E. e
ae?., 1988].

Aiino aieeo a c?aceao aecia/aee ca iaoiaeii Eio?i i niiaaao. o
iiaeeoieaoei? Iieea?a [Miller G.L., 1959].

Noaoenoe/io ia?iaeo ?acoeueoaoia i?iaiaeeee ca iaoiaeii
Nouethaeai-oa-Oioa?a [Oa?aioueaa I.A. e ae?., 1977].

INIIAII ?ACOEUeOAOE AeINEIAeAEAIIss

Aieea ioaii? na?oey ia iioaineaiinoue ia?aeeniiai ieeneaiiy ei-iiaeia i
eiai oa?iaioaoeaio ?aaoeyoeith o uo?ia ?iciiai aieo. Aeineiaeaeaiiy
iioaineaiinoi IIE, yeo oa?aeoa?ecoaaee ii iaeiie/aiith IAeA i
iioaineaiinoi oaiiethiiianoeaioei?, a aiiiaaiaoao na?oey uo?ia ?iciiai
aieo iieacaei, ui a eiio?iei niinoa?iaaeinue inoioia cieaeaiiy c
aieii iioaineaiinoi anei?aao-iiaeoeiaaiiai IIE. Oei ?acoeueoaoe
oc-aiaeaeothoueny c ?aiio iaea?aeaieie a iaoie eaai?aoi?i? aeaieie
[Eaiaoei A.A. e ae?., 1987]. Aeeiaiiea oaeaeeinoi iaeiie/aiiy IAeA aoea
yeinii noiaeith c aeeiaiieith ciiie iioaineaiinoi oaiiethiiianoeaioei?.

Iiney 3 aiaeei ia?a?aaii? ioaii? iioaineaiinoue anei?aao-iiaeoeiaa-iiai
IIE ?icei caieueooaaeany aiaeiinii eiio?ieth: a 1,4 — 1,5 ?ace — o
1-iiny/ieo i aieueo iiae o 5 ?acia — o 12- i 24-iiny/ieo uo?ia
(oaae. 1). Noiaei aieiai iniaeeainoi aieeao ioaii? ia iioaineaiinoue IIE
aoee iieacaii i aeey ia/iiee [Ieeeo/aiei TH.A. e ae?., 1997].

6

Oaaeeoey 1 Iaeiie/aiiy IAeA i iioaineaiinoue oaiiethiiianoeaioei? i?e

anei?aao-iiaeoeiaaiiio IIE o aiiiaaiaoao na?oey uo?ia ?iciiai aieo

a eiio?iei i iiney 3 aiaeei ia?a?aaii? ioaii? (n = 5 — 7)

————————————————————————
——————————Aa?iaioe :
Aie, iin

aeineiaeo
:————————————————————————
———-

: 1 : 12 :
24

————————————————————————
——————————

Iaeiie/aiiy IAeA, iiieue IAeA/ia aieeo ca 10 oa iieoaaoei?

Eiio?ieue 7,7 1,1 0,8 0,5
1,4 0,7

Ioaiiy 11,8 1,4 (a) 6,2 2,3 (a) 8,3
2,1 (a)

Iioaineaiinoue oaiiethiiianoeaioei?, oen. iii./ia aieeo

ca 10 oa iieoaaoei?

Eiio?ieue 10,0 1,6 1,7 0,6
2,2 0,8

Ioaiiy 14,4 0,9 (a) 8,7 2,4 (a) 10,0
2,5 (a) I?eiioea: a — ? < 0,05 aiaeiinii eiio?ieth Aeey c'ynoaaiiy aieiaeo iniaeeainoae iioeiaeaeaiiy oeaiei i?e ioaii? ? aaaeeeaei ieoaiiy i?i oa, a yeie ii?i aieueo aeniea iioai-neaiinoue IIE o na?oei iieiaeeo uo?ia o eiio?iei aiaeaeaa?oueny ia nooia-ith ioaii/iiai iioeiaeaeaiiy iaia?ai. E?eoa?i?i ioaii/iiai iioeiaeaeaiiy iaia?ai ea?aeiiiiioeeoia iiaea aooe aeoiae eaeoaoaeaaiae?iaaiace (EAeA) ic icieueiaaiiai ia?oociaaiiai na?oey [Coudray C. et al., 1995]. Anoaiia-eaii, ui aeeiaiiea aeoiaeo EAeA o iieiaeeo i noa?eo uo?ia iiiioii aiae?iciythoueny. O ?aiii no?iee ioaii? - ?aia?ooci? (20 - 40 oaeeei) nooiiiue iioeiaeaeaiiy iaia?ai ea?aeiiiiioeeoia aoa o 1,5 - 2,2 ?ace aeua o 1-iiny/ieo uo?ia, iiae o 24-iiny/ieo, oiaei ye i?e aieueo o?eaaeie ioaii? - ?aia?ooci? (100 oaeeei) - o noa?eo aeua a 1,7 ?ace o ii?ia-iyiii c iieiaeeie ianoaoo?aic?ieeie oaa?eiaie. Iaeneiaeueiee aeoiae oa?iaioo ic na?aeaoeue oaa?ei onio aeineiaeaeaieo a?oi aiaeaoaaany iiae 80 i 100 oaeeeiaie ia?a?aaii? ioaii?. C iaoith c'ynoaaiiy iineiaeiaiinoi i i?e/eiii-ianeiaeiiai ca'yceo iiae ie?aieie eaieaie ioaii/iiai iioeiaeaeaiiy ea?aeiiiiioeeoia caeaaaeiny aaaeeeaei aeineiaeaeaiiy ciiie iioaineaiinoi IIE i?e ia?a?aaiie ioaii? ?icii? o?eaaeinoi. Onoaiiaeaii, ui aaea 20-oaeeeiia ioaiiy i?ecaiaeeea aei caieueoaiiy iioaineaiinoi OEI o 12-iiny/ieo uo?ia aieueo, iiae o 2 ?ace aiaeiinii eiio?ieth. O 1-iiny/ieo uo?ia aiaeiinii ciiie aoee cia/-ii ieae/eie. I?e ii?iaiyiii aeaieo ii aeoiaeo EAeA i iioaineaiinoi OEI 7 iiaeia c?iaeoe aeniiaie, ui aeoeaaoeiy IIE i?e ioaii? na?oey cia/ii ia-?aaeo? i, i/aaeaeii, a cia/iie ii?i coiiaeth? iioeiaeaeaiiy na?eieaie. Aeoeaaoeith aieueii?aaeeeaeueiiai ieeneaiiy eiiiaeia i?e ioaii? oeaiei /anoi iia'ycothoue c iineaaeaiiyi aioeieneaeaioiiai caoenoo [Iaa?nii O.C., 1984; Aeeaiei I.A., 1989; Kirshenbaum L.A. et al., 1993; Porreca E. et al., 1994]. A eioa?aoo?i ? aeinoaoiuei ia?aeiieeai aeaii, yei naiae/aoue, ui i?e ioaii? ciaioo?oueny aiino i?aeoe/ii anio aee?i- i aiaei?ic/eiieo aioeieneaeaioia [Yoshida S. et al., 1982; Iaa?nii O.C.,1984; Aeeaiei I.A., 1989; ]. Iaie oaeiae anoaiiaeaii cieaeaiiy aioeieenethaaeueii? aeoeaiinoi eiiiaeia iaia?ai ( ui ? iieacieeii noaio iaoa?iaioaoeaii? aioeieneaeaioii? nenoaie) aaea ia 40 oa ioaii? na?oey, yea i?e ia?a?aaiie ioaii? aoei aieueo ae?aaeaiei, iiae i?e oioaeueiie. Aiaeiinii noaio oa?iaioaoeaii? aioeieneaeaioii? nenoaie i?e ioaii? iiiea?aeo aeaii eioa?aoo?e aeinoaoiuei noia?a/ii. Iaea?aeaii a ?acoeueoaoi aeineiaeaeaiiy aieeao ioaii? ia oa?iaioaoeaio aioeieneaeaioio nenoaio iiiea?aeo uo?ia aeaii (oaae.2) naiae/aoue, ui a eiio?iei aeooaoiiiia?ieneaeacia i aeooaoiii-S-o?ainoa?acia aeoeaiinoi o noa?eo uo?ia aoee ai?iaiaeii aeua, iiae o iieiaeeo oaa?ei. Oei ?acoeue-oaoe ocaiaeaeothoueny c ?aiio iaea?aeaieie a iaoie eaai?aoi?i? [Eaiaoei A.A. e ae?., 1987] i iiaeooue iiynithaaoe aeyaeaia cieaeaiiy eiiiia?aeen-iiai iioaioeiaeo iiiea?aeo c aieii oaa?ei. 30-oaeeeiia oioaeueia ioaiiy ia i?ecaiaeeea aei ai?iaiaeii? ciiie aeoeaiinoi aeineiaeoaaieo oa?iaioia a aeiaeiie aieiaie a?oii. 120-oaeeeiia ioaiiy aeeeeeaea caieueoaiiy NIAe aeoeaiinoi o 3-iiny/ieo uo?ia a 1,6 ?ace o ii?iaiyiii c eiio?ieai i 30-oaeeeiiith ioaii?th. I?e oeueiio o iieiaeeo oaa?ei niinoa?iaaeiny oa-eiae inoioia caieueoaiiy aeooaoiiiia?ieneaeacii? aeoeaiinoi i oaiaeaioeiy aei caieueoaiiy eaoaeacii? aeoeaiinoi. O noa?eo oaa?ei ioaiiy i?aeoe/-ii ia i?ecaiaeeea aei ciiie aeoeaiinoi aea/aieo aioeieneaeaioieo oa?-iaioia. I?e aeineiaeaeaiii aieeao ioaii? ia aeooaoiii?aaeoeoacio, ici-oeeo?ao-, iaeao- i aetheici-6-oinoaoaeaaiae?iaaiacio aeoeaiinoi iiiea?aeo uo?ia anoaiiaeaii, ui 30-oaeeeiia ioaiiy ia i?ecaiaeeea aei ciiie aea-/aieo aeoeaiinoae o uo?ia 3- i 24-iiny/iiai aieo. A i?e aieueo o?e-aaeie ioaii? (120 oa) niinoa?iaaeiny ai?iaiaeia caieueoaiiy icioeeo?ao-aeaaiae?iaaiacii? aeoeaiinoi a iiiea?aei 3-iiny/ieo uo?ia. A aeaiie ?iaioi aoei oaeiae aeineiaeaeaii aieea aiaeiinii aieueo aei?n-oeiai ioaii/iiai iioeiaeaeaiiy, a naia ioaii?-?aia?ooci?, ia noai oa?-iaioaoeaii? aioeieneaeaioii? nenoaie i anoaiiaeaii, ui aaea ia 40 oa ia?a?aaii? ioaii? niinoa?iaaeany oaiaeaioeiy aei caieueoaiiy NIAe i aeo- 8 Oaaeeoey 2 Aieea oioaeueii? ioaii? na?oey uo?ia ?iciiai aieo ia aeoeaiinoue aioeieneaeaioieo oa?iaioia (n = 5 - 8) ------------------------------------------------------------------------ ----------------------------- Aie, : O?eaaeinoue ioaii?, oa iin :----------------------------------------------------------------------- ------------------- : 0 (eiio?ieue) : 30 : 120 ------------------------------------------------------------------------ ----------------------------- Noia?ieneaeaeiniooacia aeoeaiinoue, iae. aeo./oa.ia aieeo 3 100,2 9,1 99,8 5,5 156,8 13,9 (a,a) 24 96,6 10,8 125,7 9,4 (a) 116,2 9,9 Eaoaeacia aeoeaiinoue, ieiieue H2O2/oa.ia aieeo 3 8,04 0,80 9,39 0,72 10,85 1,04 (a) 24 9,02 0,51 8,43 0,73 7,65 0,76 Aeooaoiiiia?ieneaeacia aeoeaiinoue c I2I2, iiieue NADPH/oa.ia aieeo 3 58,9 7,3 69,8 10,1 94,1 12,5 (a) 24 204,5 25,9 (a) 228,8 42,8 171,0 21,3 Aeooaoiiiia?ieneaeacia aeoeaiinoue c aiae?iia?aeenii eoiiea, iiieue NADPH/oa.ia aieeo 3 75,5 5,2 95,0 13,5 106,2 13,0 (a) 24 232,4 30,6 (a) 255,3 44,8 199,1 24,8 Aeooaoiii-S-o?ainoa?acia aeoeaiinoue, iiieue OAeIA/oa.ia aieeo 3 22,0 1,9 19,3 1,4 18,6 1,3 24 28,1 1,4 (a) 26,2 1,8 23,0 2,8 Aeooaoiii?aaeoeoacia aeoeaiinoue, iiieue NADPH/oa.ia aieeo 3 5,58 0,43 5,50 0,71 5,75 0,69 24 6,01 0,63 5,09 0,72 4,79 0,56 Icioeeo?aoaeaaiae?iaaiacia aeoeaiinoue, iiieue NADPH/oa.ia aieeo 3 1063,2 81,2 1199,3 97,4 1457,4 124,9 (a) 24 1150,2 115,1 1300,0 84,2 1167,8 113,1 Iaeaoaeaaiae?iaaiacia aeoeaiinoue, iiieue NADPH/oa.ia aieeo 3 16,1 1,23 17,02 1,04 17,25 1,36 24 20,7 1,21 (a) 18,89 1,68 17,70 1,52 Aetheici-6-oinoaoaeaaiae?iaaiacia aeoeaiinoue, iiieue NADPH/oa.ia aieeo 3 2,73 0,24 3,21 0,37 3,64 0,48 24 3,60 0,20 (a) 3,60 0,19 3,94 0,66 I?eiioee: a - ? < 0,05 aiaeiinii eiio?ieth; a - ? < 0,05 aiaeiinii 30 oa ioaii?; a - ? < 0,05 aiaeiinii 3 iin; a - 0,05 < ? < 0,1 aiaeiinii eiio?ieth. 9 oaoiiiia?ieneaeacii? (c aiae?iia?aeenii eoiiea) aeoeaiinoae i caieue-oaiiy eaoaeacii? i Se-caeaaeii? aeooaoiiiia?ieneaeacii? aeoeaiinoae a 1,5 i 1,3 ?ace, aiaeiiaiaeii, o aiaeiioaiii aei eiio?ieth. Aeo-oaoiii-S-o?ainoa?acia aeoeaiinoue i?e ia?a?aaiie ioaii?, ye i i?e oi-oaeueiie, inoioii ia ciiithaaeanue ia ?aiiie noaaei? ioaii?. I?e caieue-oaiii /ano ioaii? aei 60 oa niinoa?iaaeiny iiaeaeueoa caieueoaiiy eaoa-eacii? aeoeaiinoi oiaei, ye Se-caeaaeia aeooaoiiiia?ieneaeacia ae-oeaiinoue cieaeoaaeanue aei ?iaith eiio?iey. Aeoeaiinoue oa?iaioia, yei caaacia/othoue aiaeiiaethaaeueieie aeaiaaeaioaie aeooaoiii-caeaaeio aioe-ieneaeaioio nenoaio na?oey, ia ?aiiie noaaei? ia?a?aaii? ioaii? inoioii ia ciiithaaeanue. I?e aieueo o?eaaeie ioaii? aeooaoiii?aaeoeoacia ae-oeaiinoue cieaeoaaeanue a 1,5 ?ace, a icioeeo?aoaeaaiae?iaaiacia - caieue-ooaaeanue a 1,2 ?ace o ii?iaiyiii c eiio?ieai. Iaea?aeaii ?acoeueoaoe aeicaieythoue c?iaeoe aeniiaie, ui aeoeaiinoi aea/aieo aioeieneaeaioieo oa?iaioia a na?oei ia ?aiiie noaaei? ioaii? ia ciiiththoueny /e iaaioue caieueoothoueny. Oaeei /eiii, ?icaeyiooi aeaii naiae/aoue, ui aaea ia 20 oa ioaii? niinoa?iaa?oueny aeoeaaoeiy IIE, yea aiaeaoaa?oueny ia?aae iioeiaeaeaiiyi iaia?ai ea?aeiiiiioeeoia. I?e oeueiio aiaeiinia aeoeaaoeiy IIE i iioeiae-aeaiiy na?eieaie o aiaeiiaiaeue ia aeith ioaii? aoee aieueo ae?aaeaieie o noa?eo oaa?ei. Aiaenooiinoue inoioii? ciiie oa?iaioaoeaii? aioeiene-aeaioii? nenoaie i?e ioaii? iiaea naiae/eoe i?i iiaeeeainoue aoaeoeaiiai i?ioeioaii/iiai caoenoo na?oey oeyoii aeiaeaoeiaiai caaacia/aiiy oei?? nenoaie i?e?iaeieie iaoaaieioaie, yei iiaeaeuothoue ?iaaiue aeiii?ia aiaeiiaethaaeueieo aeaiaaeaioia (NADPH i GSH). Aieiai iniaeeainoi aieeao ioaii? iiiea?aeo ia no?oeoo?-ii-ooieoeiiiaeueiee noai iioioiiae?ie na?oey uo?ia. Iaeiei c ianeiaeeia aeoeaaoei? IIE ia ?aiiie noaaei? ioaii? iiaea aooe ciiia no?oeoo?-ii-ooieoeiiiaeueiiai noaio iioioiiae?ie i, ye ?acoeueoao, ii?ooaiiy aia?aicaaacia/aiiy iiiea?aeo. Aeineiaeaeaiiy aieeao ioaii? ia aeeoaiiy oa ieenethaaeueia oinoi?eethaaiiy iioioiiae?ie na?oey iieiaeeo i noa?eo uo?ia iieacaei (oaae. 3), ui aaea /a?ac 30 oa ioaii? a iioioiiae?iyo iiiea?-aeo noa?eo (24 iin) uo?ia niinoa?iaaeiny ai?iaiaeia ciaioaiiy oaeae-einoi aeeoaiiy a noaii 3 i?e ieeneaiii noeoeeiaoo. Inoioia ciaioaiiy aeaiiai ia?aiao?o o iieiaeeo oaa?ei aiaeaoaaeiny eeoa ia 120 oa oioaeue-ii? ioaii?. sseinii noiaeei /eiii ciiithaaeinue i aeeoaiiy a ?ic'?aeiaiiio noaii (V3?). V4 inoioii ia ciiithaaeanue. Aaee/eia AeE o noa?eo uo?ia ai?iaiaeii cieaeoaaeanue ia 30 oa, a o iieiaeeo - ia 120 oa ioaii?. 10 Ia aiaeiiio aiae noeoeeiaoo, i?e ieeneaiii aeooaiaoo c iaeaoii niinoa?iaaeiny ciaioaiiy V4 o ii?iaiyiii c eiio?ieai aaea ia 30 oa oioaeueii? ioaii? a 1,4 i 1,6 ?ace aeey 3- i 24-iiny/ieo uo?ia, aiaeiiaiaeii. Ia 120 oa niinoa?iaaeiny iiaeaeueoa ciaioaiiy oeueiai ia?a-iao?o, i?e/iio aieueo ae?aaeaia - o noa?eo uo?ia. Oa?aeoa? aiaeiiaiaei V3 e V3? ia aeith ioaii? i?e ieeneaiii aeooaiaoo c iaeaoii ia aiae?iciyany yeinii aiae oaeiai i?e ieeneaiii noeoeeiaoo. Cieaeaiiy ae-oeaiinoi aeeoaeueiiai eaioethao iioioiiae?ie i?e ieeneaiii aeooaiaoo c iaeaoii ia aieueo ?aiiie noaaei? ioaii? na?oey, iiae i?e ieeneaiii noe-oeeiaoo, iiaea aooe iiyniaiei aieueoith /ooeeainoth aei aei? ioaii? aeeoaeue-iiai eiiieaeno NADH-aeaaiae?iaaiaca - oaioiiii [Rouslin W., 1983]. Ii?ooaiiy aeeoaiiy oa ieenethaaeueiiai oinoi?eethaaiiy iioioiiae?ie i?e ioaii? iiaea aooe iia'ycaiei ia oieueee ci cieaeaiiyi aeoeaiinoi aeanii aeeoaeueiiai eaioethao iioioiiae?ie, aea i c iioeiaeaeaiiyi aaciina-?aaeiuei AOO-neioaoaciiai eiiieaeno. Aeienii, ic iaaaaeaieo a oaae. 3 aeaieo aeaeii, ui AeIO-noeioeueiaaia AOOacia aeoeaiinoue aiiiaaiaoo iiiea?aeo a na?aaeiaeui iieoaaoei? aac Mg2+ o noa?eo oaa?ei cieaeoaaeanue aaea ia 30 oa oioaeueii? ioaii?. O iieiaeeo uo?ia ai?iaiaeia cieaeaiiy aeaii? aeoeaiinoi niinoa?iaaeiny ee-oa ia 120 oa ioaii?. Aeoeaaoeiy ?iaioe eiiieaeno aeiaeaaaiiyi a na?aaei-aeua iieoaaoei? Mg2+ iiaaethaaea aieea ioaii? ia aeaio aeoeaiinoue o ii-eiaeeo uo?ia, o noa?eo uo?ia caeeoaeanue eeoa oaiaeaioeiy aei cieaeaiiy oieueee iiney 120 oa ioaii?. *ooeeaei iieacieeii ciiie no?oeoo?ii-ooieoeiiiaeueiiai noaio iioi-oiiae?ie i?e ioaii? [Eaiaoei A.A. e ae?., 1989] ? eiaoioeiaio noeioeth-aaiiy AOOacii? aeoeaiinoi 2,4-aeiiio?ioaiieii. A onio aeineiaeaeaieo aieiaeo a?oiao niinoa?iaaeiny ai?iaiaeia ciaioaiiy oeueiai ia?aiao?o aaea ia 30 oa ioaii?, i?e/iio o noa?eo oaa?ei a 2,2 ?ace aiaeiinii eiio?ieth, a o iieiaeeo - oieueee a 1,8 ?ace (a na?aaeiaeui iieoaaoei? aac Mg2+). Aeineiaeaeaiiy ieiaiiioeei/ooeeai?, naia iioioiiae?iaeueii?, AOOacii? aeoeaiinoi aeicaieeei anoaiiaeoue, ui ?? ciiia i?e ioaii? ao-ea yeinii noiaeith ci ciiiith caaaeueii? AeIO-noeioeueiaaii? AOOacii? ae-oeaiinoi, aea aieueo ae?aaeaiith. Ieiaiiioeei?acenoaioia, ia iioi-oiiae?iaeueia, AOOacia aeoeaiinoue neeaaeaea eeoa iaeio i'yoo /anoeo aiae caaaeueii? AeIO-noeioeueiaaii? AOOacii? aeoeaiinoi i inoioii ia ciiith-aaeany i?e oioaeueiie ioaii?. Oei aeaii iiaeooue naiae/eoe i?i oa, ui iioioiiae?iaeueia AOOaca aiineoue iniiaiee aeeaae a AeIO-noeioeueiaaio AOOacio aeoeaiinoue eeioeie i, i/aaeaeii, naia aiia a ia?oo /a?ao ca- 11 cia? aieea ioaii?. Oaaeeoey 3 Aieea ioaii? na?oey ia aeeoaiiy, ieenethaaeueia oinoi?eethaaiiy (* - iaoii I/oa.ia aieeo) i AOOacio aeoeaiinoue (iiieue Oi/oa.ia aieeo) aiiiaaiaoia iiiea?aeo iieiaeeo i noa?eo uo?ia (n = 8 - 10) ------------------------------------------------------------------------ ----------------------------- Aeineiaeaeoaaiee: O?eaaeinoue ioaii?, oa ia?aiao? :----------------------------------------------------------------------- -------- : 0 (eiio?ieue) : 30 : 120 ------------------------------------------------------------------------ ----------------------------- 3-iiny/ii uo?e V4 * 51,3 4,4 45,0 3,6 42,1 5,3 V3 * 103,8 8,8 89,6 7,4 57,8 6,2 (a) V3?* 103,5 11,1 91,2 7,7 63,7 5,9 (a) AeE 2,27 0,08 2,07 0,07 1,44 0,09 (a) 24-iiny/ii uo?e V4 * 51,1 4,6 42,6 2,8 45,9 4,3 V3 * 117,2 8,6 77,2 3,1 (a) 68,0 8,7 (a) V3?* 122,0 10,7 73,6 6,1 (a) 69,8 8,6 (a) AeE 2,32 0,05 1,94 0,08 (a) 1,61 0,08 (a) AeIO-noeioeueiaaia AOOacia aeoeaiinoue 3-iiny/ii uo?e aac Mg2+ 259,6 19,7 266,7 21,5 191,4 22,4 (a) c Mg2+ 285,3 15,0 285,2 19,5 275,1 15,6 24-iiny/ii uo?e aac Mg2+ 291,7 15,5 244,0 17,1 (a) 174,6 11,5 (a,a) c Mg2+ 302,7 16,0 294,1 14,9 252,3 18,1 (a) Ieiaiiioeei/ooeeaa AeIO-noeioeueiaaia AOOacia aeoeaiinoue 3-iiny/ii uo?e aac Mg2+ 210,5 23,6 216,3 15,7 143,1 19,4 (a,a) c Mg2+ 161,4 9,0 155,6 11,7 148,0 14,0 24-iiny/ii uo?e aac Mg2+ 230,3 14,6 180,2 16,5 (a) 120,3 8,8 (a,a) c Mg2+ 183,0 9,9 145,7 8,0 (a) 120,2 12,9 (a,a) I?eiioee: a - P < 0,05 aiaeiinii eiio?ieth; a - 0,05 < ? < 0,1 aiaeiinii eiio?ieth; a - ? < 0,05 aiaeiinii 30 oa ioaii?; a - 0,05 < ? < 0,1 aiaeiinii 30 oa ioaii? 12 Ciiia ooieoeiiiaeueiiai noaio iioioiiae?ie i?e aei? ?icieo oice/ieo i oiii/ieo oaeoi?ia, a oaeiae i?e ?yaei iaoieiai/ieo noaiiaeu i, cie-?aia, i?e ioaii? iiaea i?ecaiaeeoe aei aenieiaiieiooaeiiai iaaooaiiy i?-aaiae, nooiiiue yeiai, ye i?aaeei, caeaaeeoue aiae iioaineaiinoi i o?e-aaeinoi aei? oaeoi?o /e aaaeeinoi caoai?thaaiiy [Schmiedl A. et al., 1990]. I?iaaaeaii aeineiaeaeaiiy iieacaee, ui 30-oaeeeiia ioaiiy i?ecaiaeeea aei ciaioaiiy /ano iaiiaiaaooaiiy iioioiiae?ie o noa?eo uo?ia a 1,5 ?ace o ii?iaiyiii c eiio?ieai. O iieiaeeo uo?ia ai?iaiaeia cieaeaiiy aeaiiai ia?aiao?o niinoa?iaaeiny eeoa ia 120-e oa ioaii?. Noiaea aiei-aa ni?yiiaaiinoue ciiie /ano iaiiaiaaooaiiy o aiaeiiaiaeue ia aeith ioaii? iaea?aeaia ?aiio a iaoie eaai?aoi?i? oaeiae i aeey iioioiiae?ie ia/iiee uo?ia [Oaeiaeeiaa A.A., 1994]. Ca aeoieith ?yaeo aaoi?ia, iaeii?th c iniiaieo i?e/ei caieueoaiiy i?i-ieeiinoi aioo?ioiuei? iaia?aie iioioiiae?ie i, ye ianeiaeie, ?icaeoeo iaaooaiiy, ? ciiia no?oeoo?e iaia?aie ca ?aooiie aeoeaaoei? IIE [Non-eiaa A.E. e ae?., 1988; Carbonera D. et al., 1988], yea a iaoeo aeineiaeaeaiiyo niinoa?iaaeany aaea ia 20 oa ioaii?. I?e/eiaie aeniei-aiieiooaeiiai iaaooaiiy iioioiiae?ie iiaeooue aooe oaeiae aioo?ieeioeiiee aoeeaeic, iaeiie/aiiy i?iaeoeoia ?iciaaeo aaeaiiiioeeaioeaeia oa iioi oae-oi?e [Crompton M. et al., 1990]. Aeienii, iaie aeyaeaii, ui aaea ia 10 oa oioaeueii? ioaii? iiiea?aeo uo?ia niinoa?iaa?oueny ai?iaiaeia caieue-oaiiy eiioeaio?aoei? i?ioiiia i oinoaoa iai?aaii/iiai. Oaeei /eiii, iaaaaeaii aeaii i?i no?oeoo?ii-ooieoeiiiaeueiee noai iioioiiae?ie na?oey iieiaeeo i noa?eo uo?ia naiae/aoue, ui i?e oioaeueiie ioaii? na?oey uo?ia ciiia iioaineaiinoi aeeoaiiy i ieenethaaeueiiai oin-oi?eethaaiiy, AOOacii? aeoeaiinoi i nooiaith aenieiaiieiooaeiiai iaao-oaiiy iioioiiae?ie aoea aieueo ae?aaeaiith o noa?eo oaa?ei. Neiae aiaecia/eoe, ui aeyaeaii aieiai iniaeeainoi aei? ioaii? ia no?oeoo?ii-ooieoeiiiaeueiee noai iioioiiae?ie aoee yeinii noiaeeie c iniaeeainoyie ciiie iioaineaiinoi IIE. Ia aeeeth/aii, ui aeoeaaoeiy IIE ia aiaeiinii ?aiiie noaaei? ioaii? iiaea ni?e/eithaaoe ii?ooaiiy nenoaie iioioiiae?iaeueiiai ieeneaiiy. Iioith, i ia iaio aaaeeeaith, i?e-/eiith oeueiai iiaea aooe iaaeinoaoiy caaacia/aiinoue iiiea?aeo i?e ioaii? noano?aoaie ieeneaiiy. I?ioeioaii/ia aeiy i?e?iaeieo iaoaaieioia. ?icaeyiooi a iiia?aaeiue-iio ?icaeiei aeaii naiae/aoue, ui aeiaeaoeiaa caaacia/aiiy na?oey i?e?iaeie-ie iaoaaieioaie, yei ? noano?aoaie iioioiiae?iaeueiiai ieeneaiiy oa 13 aeaea?aeii aiaeiiaethaaeueieo aeaiaaeaioia aeey NADPH-GSH-caeaaeii? aioeie-neaeaioii? nenoaie, iiaea aooe aoaeoeaiei caniaii caoenoo iiiea?aeo aiae ioaii/iiai iioeiaeaeaiiy. C oei?th iaoith a aeaiie ?iaioi aoei i?iaaaeaii aeineiaeaeaiiy i?i-oeioaii/ieo aeanoeainoae ?yaeo i?e?iaeieo iaoaaieioia. I?ioeioaii/io aeith aeineiaeaeoaaieo ?a/iaei oa?aeoa?ecoaaee ii ?o caeiaiinoi oiiaieueith-aaoe aeoiae EAeA ic ea?aeiiiiioeeoia i?e ia?a?aaiie ioaii? i ciaiooaaoe iaeiie/aiiy iaeiiai c eiioeaaeo i?iaeoeoia IIE - IAeA. A ?acoeueoaoi i?iaaaeaieo aenia?eiaioia aoei anoaiiaeaii, ui c onio aeineiaeaeaieo iaoaaieioia, yei aiaeiiaiaeathoue ca aaia?aoeith aiaeiia-ethaaeueieo aeaiaaeaioia a eeioeii (icioeeo?ao, aetheici-6-oinoao, 6-oinoiaetheiiao, iaeao, GSH), iaeaieueo aoaeoeaio i?ioeioaii/io aeith iaa icioeeo?ao. Oae, cie?aia, aeiaeaaaiiy icioeeo?aoo a eiioeaio?aoei? 5 iI a na?aaeiaeua ia?ooci? na?oey i?ecaiaeeei aei ciaioaiiy aeoiaeo EAeA ic ea?aeiiiiioeeoia ia 100-e oa ioaii? a 1,8 ?ace o ii?iaiyiii c eiio?i-eai i ciaioaiith iaeiie/aiiy IAeA /a?ac 3 aiaeeie ioaii? - ?aia?ooci? a 1,4 ?ace. Aeaii eioa?aoo?e i?i oa, ui iniiaio ?ieue a iiaeo?eioaaiii ?iaith NADPH o iiiea?aei a?a? icioeeo?aoaeaaiae?iaaiaca [Pfeifer R. et al., 1986], aeicaieythoue iiynieoe niinoa?aaeaio aoaeoeaio i?ioeioaii/io aeith icioeeo?aoo. Aeineiaeaeaiiy iioeo i?e?iaeieo iaoaaieioia (aetheici-1-oinoao, aeo-oaiao, -eaoiaeooa?ao, noeoeeiao, ii?oaao, aetheica) aeicaieeei anoaii-aeoe, ui aeinoaoiuei ae?acio i?ioeioaii/io aeith iaee aeooaiao, ii?oaao i aetheica, yei i?e aeiaeaaaiii a na?aaeiaeua ia?ooci? a eiioeaio?aoei? 5 iI ciaiooaaee aeoiae EAeA ic ea?aeiiiiioeeoia ia 100-e oa ioaii? - ?a-ia?ooci? a 1,6, 1,8 i 2,2 ?ace, aiaeiiaiaeii, o ii?iaiyiii c eiio?i-eai. I?e/iio, oni aeineiaeaeaii i?e?iaeii iaoaaieioe, yei caeaoii aoae-oeaii oiiaieueithaaoe aeoiae EAeA ic ea?aeiiiiioeeoia, inoioii ciaiooaaee i iaeiie/aiiy IAeA. Iaea?aeaii aeaii noaee iiaenoaaith aeey aeineiaeaeaiiy aieeao eiiieaenia ic 2 /e 3 iaeaieueo aoaeoeaieo iaoaaieioia ia nooiiiue ioaii/iiai iio-eiaeaeaiiy na?oey. Naiei aoaeoeaiei ic ieo aeyaeany eiiieaen, yeee neeaaea?oueny ic aeooaiaoo, icioeeo?aoo i ii?oaaoo o aiaeiiaiaeieo eieue-einoyo. Aeaii, iaaaaeaii a oaae. 4, naiae/aoue, ui eiiieaen iaoaaieioia a eiioeaio?aoei? 1,0, 2,5 i 5,0 iI (eiaeiiai ic iaoaaieioia) ai?iaiaeii cieaeoaaa aeoiae EAeA a ia?oocao. I?e oeueiio iaeaieueo aoaeoeaiei aoa eiiieaen iaoaaieioia a eiioeaio?aoei? 2,5 iI. Oae, ia 100-e oa ioaii? oeae eiiieaen oiiaieueithaaa aeoiae EAeA a 3,4 ?ace aiaeiinii eiio?ieth. 14 Oaaeeoey 4 Aieea noiioia i?e?iaeieo iaoaaieioia ia aeoiae eaeoaoaeaaiae?iaaiace i?e ia?a?aaiie ioaii? icieueiaaiiai na?oey uo?ia (a % aei aeoiaeo oa?iaioo ia 180-e oa ioaii?; n = 6 - 22) ------------------------------------------------------------------------ ----------------------------- Aeineiaeaeoaaii : O?eaaeinoue ioaii?, oa iaoaaieioe :----------------------------------------------------------------------- -- : 20 : 60 : 100 : 140 : 180 ------------------------------------------------------------------------ ----------------------------- Eiio?ieue 7,10,8 21,12,0 59,93,1 91,23,6 100 Icioeeo?ao + ii?oaao + aeooaiao 0,4 iI eiaeiiai 5,80,6 19,21,9 54,88,5 92,02,8 100 1,0 iI eiaeiiai(a) 2,70,2 7,20,3 26,90,9 62,32,3 100 2,5 iI eiaeiiai(a) 2,00,7 5,20,9 17,52,8 57,74,9 100 5,0 iI eiaeiiai(a) 2,00,5 5,30,3 19,84,7 69,77,3 100 10,0 iI eiaeiiai 6,20,6 21,22,9 59,35,7 92,46,8 100 Aetheica 5,0 iI (a) 4,11,2 8,92,0 27,63,5 62,75,6 100 2,5 iI icioeeo?ao + 2,5 iI ii?oaao + 2,5 iI aeooaiao + 5,0 iI aetheica(a,a) 1,40,5 3,20,5 13,41,6 47,67,2 100 I?eiioee: a - ? < 0,05 aiaeiinii eiio?ieth; a - ? < 0,05 aiaeiinii "iio?ieiiai" eiiieaeno (1 - 5 iI). Nooiiiue caoenii? aei? "iio?ieiiai" eiiieaeno caieueooaaeany i?e aeiiia-ithaaiii eiai 5 iI aetheice. A oeueiio aeiaaeeo aeoiae EAeA a ia?oocao ia 100-e oa ia?a?aaii? ioaii? cieaeoaaany a 1.3 ?ace aeiaeaoeiai o aiaeii-oaiii aei oaeiai i?e aeei?enoaiii oieueee "iio?ieiiai" eiiieaeno i a 4,5 ?ace - o aiaeiioaiii aei eiio?ieth. Aeiiiaiaiee aetheicith eiiieaen iaoaaieioia i?ecaiaeea aei ai?iaiaeiiai cieaeaiiy ?iaith IAeA a 1,9 ?ace o ii?iaiyiii c eiio?ieai i a 1,4 ?ace o ii?iaiyiii c ?iaiai IAeA, yeee ca?a?no?iaaii aeey na?aeaoeue, yei ia?oocoaaee c aeiaeaaaiiyi oieueee aeth-eice. A iaoeo aeineiaeaeaiiyo oaeiae iieacaii, ui oeae eiiieaen iie?auo-aaa iiiiaeaiiy nei?i/oaaeueii? aeoeaiinoi na?oey ia aiaeiinii ?aiiie noaaei? ioaii?-?aia?ooci? i ciaiooaaa iaa?ye iiiea?aeo. Oaeei /eiii, i?iaaaeaii aeineiaeaeaiiy naiae/aoue, ui ?ic?iaeaiee eiiieaen i?e?iaeieo iaoaaieioia (5 iI aetheice i ii 2,5 iI icioeeo?aoo, 15 ii?oaaoo i aeooaiaoo) cia/ii oiiaieueith? ioaii/ia iioeiaeaeaiiy na?oey. Ia iiaenoaai oeeo aeaieo aoea iiaeaia cayaea ia aeiaoiae i io?eiaii aa-oi?nueea naiaeioeoai ia na?aaeiaeua, yea iiia?aaeaeo? ioaii/ia iioeiaeaeaiiy oeaiei i?e o?ainieaioaoei? i?aaiia (A.n. SU 1632427 A1, A 61 E 9/08). AENIIAEE 1. I?iaaaeaii ii?iaiyeueiee aiaeic ciiie iioaineaiinoi ia?aeeniiai ieeneaiiy eiiiaeia, eiai oa?iaioaoeaii? ?aaoeyoei? oa no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie a aeeiaiioei ioaii/iiai iioeiae-aeaiiy iiiea?aeo iieiaeeo i noa?eo uo?ia i anoaiiaeaii, ui aeoeaaoeiy IIE aiaeaoaa?oueny aaea /a?ac 20 oaeeei ioaii? i ia?aaeo? ii?ooaiith aa?'??ii? ooieoei? na?eieaie. A oie aea /an aeoeaiinoue ?yaeo aioeie-neaeaioieo oa?iaioia a na?oei uo?ia ia ?aiiie noaaei? oioaeueii? oa ia?a?aaii? ioaii? ia ciiith?oueny aai iaaioue iiaeaeuo?oueny (noia?ie-neaeaeeniooacia i?e oioaeueiie ioaii?, eaoaeacia i aeooaoiiiia?iene-aeacia - i?e ia?a?aaiie ioaii?). 2. Aieiai iniaeeainoi aieeao ioaii? ia no?oeoo?ii-ooieoeiiiaeueiee noai iioioiiae?ie na?oey uo?ia aeyaeyeeny a ianooiiiio: - a cieaeaiii iioaineaiinoi aeeoaiiy a iaoaaiei/ieo noaiao 3 i 3? i aeeoaeueiiai eiio?ieth i?e ieeneaiii noeoeeiaoo oa iioaineaiinoi aee-oaiiy a noaiao 3, 3? i 4 (ca *ainii) i?e ieeneaiii aeooaiaoo c iaeaoii, yea o noa?eo uo?ia niinoa?iaaeiny ia aieueo ?aiiie noaaei? ioaii?, iiae o iieiaeeo oaa?ei; - a cieaeaiii caaaeueii? AeIO-noeioeueiaaii? oa ieiaiiioeei/ooeeai? AOOacii? aeoeaiinoae o 24-iiny/ieo uo?ia /a?ac 30 oaeeei ioaii?, a o 3-iiny/ieo - oieueee /a?ac 120 oaeeei ioaii?; - a ciaioaiii /ano iaiiaiaaooaiiy iioioiiae?ie, yea o noa?eo oaa-?ei aiaeaoaaeiny ia aieueo ?aiiie noaaei? ioaii?, iiae o iieiaeeo uo-?ia. 3. Anoaiiaeaii, ui aiaeiinia aeoeaaoeiy IIE, iioeiaeaeaiiy na?eieaie i ii?ooaiiy no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie na?oey ao-ee aieueo cia/ieie o noa?eo, 24-iiny/ieo, uo?ia. Aeoiaey/e c inia-eeainoae ciii aeineiaeaeaieo i?ioeania o aeeiaiioei ioaii/iiai iioeiae-aeaiiy, i?eionea?oueny, ui ii?ooaiiy no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie iiiea?aeo noa?eo uo?ia ia ?aiiie noaaei? ioaii? coiiaeaii aieueo ae?aaeaiith aeoeaaoei?th IIE o noa?eo oaa?ei. 16 4. Aeineiaeaeaii i?ioeioaii/ii aeanoeainoi ?yaeo i?e?iaeieo iaoaaieioia, yei ? aeiii?aie aiaeiiaethaaeueieo aeaiaaeaioia aeey oa?iaioaoeaii? aioeieneaeaioii? nenoaie i noano?aoaie iioioiiae?iaeueiiai ieeneai-iy, oa ia iniiai eiiieaeno c iaeaieueo aoaeoeaieo iaoaaieioia ?ic-?iaeaii na?aaeiaeua aeey iiia?aaeaeaiiy ioaii/iiai iioeiaeaeaiiy oeaiei (A.n. SU 1632427 A1, A 61 E 9/08). NIENIE I?AOeUe, UI AIAeIA?AAEATHOUe INIIAII IIEIAEAIIss AeENA?OAOeI? 1. Eaiaoei A.A., Ieeeo/aiei TH.A., O?iyiiaa (Aecthaa) A.I., Iaeaiaea I., Aaeinoioeeay E.E. Iniaaiiinoe eoaie/aneiai iia?aaeaeaiey iaia?ai e aeoeaaoeey ia?aeeniiai ieeneaiey eeieaeia a ieiea?aea e?un ?aciiai aic?anoa // Oe?. aeioei. aeo?i. - 1989. - O. 61,N2. - N. 98-105. 2. O?iyiiaa (Aecthaa) A.I., Ieeeo/aiei TH.A., Eaiaoei A.A. Aic?anoiua iniaaiiinoe iia?aaeaeaiey iaia?ai na?aeoea e?un i?e yenia?eiaioaeue-iie eoaiee e aiciiaeiinoue caueou i?e?iaeiuie iaoaaieeoaie // Aano-iee Oa?ueeianeiai oi-oa. - 1990. - N346. - N. 31-33. 3. Nikitchenko Yu.V., Dzuba V.N., Lemeshko V.V. Protective effect of some natural metabolites under ischemia of rat hearts // School of Fundam. Med. J. - 1996. - V. 2, N1. - P. 24-27. 4. Ieeeo/aiei TH.A., Aecthaa A.I., Eaiaoei A.A. Ia?aeeniia ieeneaiea eeieaeia e aai oa?iaioaoeaiay ?aaoeyoeey i?e eoaiee ia/aie iieiaeuo e noa?uo e?un // Aeieiae/aneee aanoiee. - 1997. - O. 1, N1. -N. 23-29. 5. A.n. SU 1632427 A1, A 61 E 9/08. N?aaea aeey i?aaeioa?auaiey eoaie-/aneiai iia?aaeaeaiey oeaiae i?e o?ainieaioaoeee i?aaiia /TH.A.Ieeeo-/aiei, A.I.O?iyiiaa (Aecthaa), A.A.Eaiaoei (NNN?). - N 4443960/14; Cayaeaii 20.06.88; Iioae. 07.03.91, Athe.N9. - 4 n. 6. Eaiaoei A.A., Ieeeo/aiei TH.A., Aecthaa A.I. Ia?aeeniia ieeneaiea eeieaeia, aai oa?iaioaoeaiay ?aaoeyoeey e ninoiyiea ieoioiiae?ee a aeeiaieea eoaiee na?aeoea e ia/aie iieiaeuo e noa?uo e?un // II iaoe. eiia?. aa?iio. e aa?eao?. Oe?aeiu. - Eeaa, 1994. - N. 388. 7. Aecthaa A.I., Oaeiaeeiaa A.A., Oiiieia O.A., Eaiaoei A.A. Aeeyiea eoaiee ia no?oeoo?ii-ooieoeeiiaeueiia ninoiyiea ieoioiiae?ee e ia?a-eeniia ieeneaiea eeieaeia na?aeoea iieiaeuo e noa?uo e?un // III iaae-aeoi. neii. "Aeieiae/aneea iaoaieciu noa?aiey". - Oa?ueeia, 1998. - N. 20. 17 AIIOAOeIss Aecthaa A.I. Aieiai iniaeeainoi aieeao ioaii? ia no?oeoo?-ii-ooieoeiiiaeueiee noai iioioiiae?ie i ia?aeenia ieeneaiiy eiiiaeia na?oey uo?ia. - ?oeiien. Aeena?oaoeiy ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa aiieiai/ieo iaoe ca niaoeiaeueiinoth 03.00.04 - aiioiiiy. - Oa?eianueeee aea?aeaaiee oiiaa?neoao, Oa?eia, 1998. Aeena?oaoeith i?enay/aii aeineiaeaeaiith iaoaiicio ioaii/iiai iioeiae-aeaiiy na?oey uo?ia. A ?iaioi aea/aii aieiai iniaeeainoi ciiie ia?a-eeniiai ieeneaiiy eiiiaeia, eiai oa?iaioaoeaii? ?aaoeyoei? oa no?oeoo-?e i ooieoei? iioioiiae?ie na?oey uo?ia i?e ioaii? i?aaio. Anoaiiaeaii, ui aeoeaaoeiy IIE i?e ioaii? ia?aaeo? ii?ooaiith aa?'??ii? ooieoei? na?-eieaie i no?oeoo?ii-ooieoeiiiaeueiiai noaio iioioiiae?ie na?oey uo?ia. Iieacaii, ui na?oea noa?eo uo?ia iaio noieea aei aei? ioaii?. Aeineiaeaea-ii i?ioeioaii/io aeith ?yaeo i?e?iaeieo iaoaaieioia i ia iiaenoaai iaea?-aeaieo aeaieo ?ic?iaeaii na?aaeiaeua aeey iiia?aaeaeaiiy ioaii/iiai iio-eiaeaeaiiy oeaiei i?e o?ainieaioaoei? i?aaiia. Eeth/iai neiaa: ioaiiy iiiea?aeo, ia?aeenia ieeneaiiy eiiiaeia, oa?iaioaoeaia aioeieneaeaioia nenoaia, aeeoaiiy i ieenethaaeueia oinoi-?eethaaiiy iioioiiae?ie, aenieiaiieiooaeia iaaooaiiy iioioiiae?ie, i?i-oeioaii/iee cania. AIIIOAOeEss Aecthaa A.I. Aic?anoiua iniaaiiinoe aeeyiey eoaiee ia no?oeoo?-ii-ooieoeeiiaeueiia ninoiyiea ieoioiiae?ee e ia?aeeniia ieeneaiea eeie-aeia na?aeoea e?un. -?oeiienue. Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.04 - aeioeiey. - Oa?ueeianeee ainoaea?n-oaaiiue oieaa?neoao, Oa?ueeia, 1998. Aeenna?oaoeey iinayuaia enneaaeiaaieth iaoaiecia eoaie/aneiai iia-?aaeaeaiey na?aeoea e?un. A ?aaioa eco/aiu aic?anoiua iniaaiiinoe ecia-iaiey eioaineaiinoe ia?aeeniiai ieeneaiey eeieaeia, aai oa?iaioaoea-iie ?aaoeyoeee e no?oeoo?u e ooieoeee ieoioiiae?ee na?aeoea e?un i?e eoa-iee i?aaia. Onoaiiaeaii, /oi aeoeaaoeey IIE i?e eoaiee i?aaeoanoaoao ia?ooaieth aa?uea?iie ooieoeee na?eieaiiu e no?oeoo?ii-ooieoeeiiaeueiiai 18 ninoiyiey ieoioiiae?ee na?aeoea e?un. Iieacaii, /oi na?aeoea noa?uo e?un iaiaa onoie/eai e aeaenoaeth eoaiee. Enneaaeiaaii i?ioeaieoaie/aneia aeaenoaea ?yaea i?e?iaeiuo iaoaaieeoia e ia iniiaaiee iieo/aiiuo aeaiiuo ?ac?aaioaia n?aaea aeey i?aaeioa?auaiey eoaie/aneiai iia?aaeaeaiey oeaiae i?e o?ainieaioaoeee i?aaiia. Eeth/aaua neiaa: eoaiey ieiea?aea, ia?aeeniia ieeneaiea eeieaeia, oa?iaioaoeaiay aioeieneaeaioiay nenoaia, aeuoaiea e ieeneeoaeueiia oin-oi?eee?iaaiea ieoioiiae?ee, aunieiaiieeooaeiia iaaooaiea ieoioiiae?ee, i?ioeaieoaie/aneia n?aaenoai. SUMMARY Dzuba V.N. Age peculiarities of ischemia effect on mitochondrial structural-functional state and lipid peroxidation of rat heart. - Manuscript. Thesis for a candidate's degree by speciality 03.00.04 -biochemistry. - Kharkov State University, Kharkov, 1998. The dissertation is devoted to investigation of mechanism of rat heart ischemic injury. Age peculiarities of changes of lipid peroxidation, its enzymatic regulation and mitochondria structure and function in rat heart during myocardial ischemia were studied. It was established that lipid peroxidation activation during ischemia precedes the damage of sarcolemmal barrier function and mitochondrial structural-functional state in rat heart. Old rat heart has proved to be less stable to ischemia effect. The anti-ischemic effect of some natural metabolites was studied and on the basis of obtained data the medium for tissue ischemic injury prevention during organ transplantation has been worked out. Key words: myocardial ischemia, lipid peroxidation, enzymatic antioxidant system, respiration and oxidative phosphorylation of mitochondria, high amplitude swelling of mitochondria, anti-ischemic remedy. I?aeienaii aei ae?oeo 09.11.98 ?. Cai. 300 Oe?aae – 100 i?ei. Oiia. ae?oe. a?e. 1.0 Oi?iao 60o841/16 OIA “Ciaiiy”, Oa?e?a, aoe. A?oaia, 32

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