32

?INOEOOO I?E?IA?IEIA?? ? A??ONIEIA??

?i.Ae.E.CAAIEIOIIAI IAI OE?A?IE

NI?IEOEIAA ??EIA AI?EN?AIA

OAeE 579.852.11: 615.331

Oai?aoe/Ia iaA?OIOOAAIIss ? i?aeoeea CANOINOAAIIss aaeoa??e ?iaeO
Bacillus aeey eiino?oTHaaiIy iiaEo i?ia?ioee?a

03.00.07 — i?e?ia?ieia?y

A A O I ? A O A ? A O

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

aeieoi?a a?ieia?/ieo iaoe

Ee?a — 1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia o a?aeae?e? aioea?ioee?a ?inoeoooo i?e?ia?ieia?? ?
a??onieia?? ?i.Ae.E.Caaieioiiai IAI Oe?a?ie

Iaoeiaee eiinoeueoaio:

aeieoi? iaaee/ieo iaoe, i?ioani?, aeaaeai?e IAI Oe?a?ie

Ni??iia Aaea??e Aai?ai?iiae/, caa?aeoaa/ a?aeae?eo aioea?ioee?a
?inoeoooo i?e?ia?ieia?? ? a??onieia?? ?i.Ae.E.Caaieioiiai IAI Oe?a?ie

Io?oe?ei? iiiiaioe:

aeieoi? a?ieia?/ieo iaoe, /e.-ei?. IAI Oe?a?ie, i?ioani? Iaoeaetho
Aiaaeai Iaaeiae/, ?inoeooo i?e?ia?ieia?? ? a??onieia??
?i.Ae.E.Caaieioiiai IAI Oe?a?ie, caa?aeoaa/ a?aeae?eo aaiaoeee
i?e?ii?aai?ci?a;

aeieoi? a?ieia?/ieo iaoe, i?ioani? Oaaei?ianueea Ieaia Ieaen??aia,
Ee?anueeee iaoeiai-aeine?aeiee ?inoeooo aaiaoieia?? oa ia?aeeaaiiy
e?ia? IIC Oe?a?ie, caa?aeoaa/ eaai?aoi??? i?e?ia?ieia?? oa ?ioiieo
i?aia?ao?a e?ia?;

aeieoi? iaaee/ieo iaoe, i?ioani? E?aiai/ooeueeee Aaiaae?e Ieeieaeiae/,
Aei?i?iiao?ianueea aea?aeaaia iaaee/ia aeaaeai?y, caa?aeoaa/ eaoaae?e
i?e?ia?ieia?? ? ai?aeai?ieia??

I?ia?aeia onoaiiaa:

Ee?anueeee iaoeiai-aeine?aeiee ?inoeooo ai?aeai?ieia?? ? ?ioaeoe?eieo
oai?ia ?i. E.A.A?iiaoaanueeiai IIC Oe?a?ie

Caoeno a?aeaoaeaoueny “ 16 ” /a?aiy 1999 ?. i 10 aiaeei? ia can?aeaii?
niaoe?ae?ciaaii? a/aii? ?aaee Ae26.233.01 ?inoeoooo i?e?ia?ieia?? ?
a??onieia?? ?i. Ae.E.Caaieioiiai IAI Oe?a?ie (252143, i.Ee?a,
aoe.Caaieioiiai, 154)

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? ?inoeoooo
i?e?ia?ieia?? ? a??onieia?? ?i. Ae.E.Caaieioiiai IAI Oe?a?ie (252143,
i.Ee?a, aoe.Caaieioiiai, 154)

Aaoi?aoa?ao ?ic?neaiee “ 11 ” o?aaiy 1999 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee,

eaiae. a?ie. iaoe
Io??o E.I.

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue oaie.

Ooiaeaiaioaeuei? aeine?aeaeaiiy no/anii? a?ieia?/ii? ? iaaee/ii? iaoee,
oni?oe a i?ciaii? aaaaoia?aiieo aniaeo?a aca?iia?aeiioaiue iae?i- ?
i?e?ii?aai?ci?a aeicaieeee ?ic?iaeoe oa ai?iaaaeeoe a i?aeoeeo
a?ii?aia?aoe-i?ia?ioeee, iniiao yeeo neeaaeathoue aeea? i?e?iai?
eoeueoo?e.

A iao /an, yeee aeaye? a/ai? (Lyons, 1986) iaceaathoue “i?eeaeaoiueith
aiioith i?ia?ioee?a”, a?ii?aia?aoe ia iniia? aeeaeo i?e?iaieo eoeueoo?
oe?iei canoiniaothoueny a iaaeeoeei? oa aaoa?eia??? aeey ei?aeoe??
i?e?ioei?e. Vanbelle et al (1990) a?aei?/athoue, ui o?eueee a e?a?iao
?AN ca?a?no?iaaii a?eueo ye 20 ??cieo i?ia?ioee?a. Iaeiae ? iio?aaa a
iiaeo a?ii?aia?aoao, iaoiiaeaia iaiaaeai?noth niaeo?a niaoeeo?/ii?
aeoeaiino? a?aeiieo i?ia?ioee?a ? ??ciiiai?oi?noth i?e?iaeieia?/ieo
ii?ooaiue ii?iaeueii? i?e?ioei?e, ui aeeeeeathoue aeenaaeoa??ic.

Ia?niaeoeaiei iai?yieii ?ic?iaee iiaeo a?ii?aia?ao?a ? canoinoaaiiy
aaeoa??e ?iaeo Bacillus. Oe? i?e?ii?aai?cie, caaaeyee aenieei
aaeaioeaiei iiaeeeainoyi, oe?iei ?iciianthaeaeai? a i?e?iae?, cie?aia, a
oeo ia’?eoao, c yeeie ethaeeia eiioaeoo? iaeo?ni?oa (oa?/ia? i?iaeoeoe,
aiaea, iia?o?y oa ?io.) (Nie?iia e ae?., 1982; Matches et al.,1987;
Odunfa, Oyewole, 1986). Caaaeyee oeueiio aaoeeee iino?eii ? a cia/i?e
e?eueeino? iaaeoiaeyoue aei i?aai?cio ethaeeie ?, ine?eueee, ? no?eeeie
aei e?oe/ieo ? o?aaieo oa?iaio?a, caa??aathoue aeeoo?caeaoi?noue ia
anueiio i?ioyc? oeoieiai-eeoeiaiai o?aeoo. Oaeei /eiii, aa?iai?
nii?iooai?thth/? aaeoa??? ?iaeo Bacillus ? iaeiei c aaaeeeaeo
eiiiiiaio?a aeciaaiii? i?e?ioei?e. Na?aae ?ioeo i?aaenoaaiee?a
aeciaaiii? i?e?ioei?e aaoeeee iathoue ?yae ia?aaaa, ye? aeicaieythoue
aaaaeaoe ?o iaeaoaeoeai?oeie aeey eiino?othaaiiy iiaeo a?ii?aia?ao?a:

1) oe? aaeoa??? (e??i B.anthracis ? B.cereus), ye i?aaeei, aacia/i? aeey
iae?ii?aai?cio;

2) na?aae ieo iiaeia a?ae?a?aoe ooaie, aioaaii?noe/ia aeoeai?noue yeeo
a?eueo ae?aaeaia ? i?iyaey?oueny uiaei a?eueo oe?ieiai niaeo?a
iaoiaaiieo oa oiiaii iaoiaaiieo i?e?ii?aai?ci?a, i?ae o ?ioeo
i?aaenoaaiee?a aeciaaiii? oa aiaeiaaiii? i?e?ioei?e;

3) aaoeeee oa?aeoa?ecothoueny aenieith oa?iaioaoeaiith aeoeai?noth, ui
iiaea iaoiiaeoe ?aaoethaaiiy oa noeioethaaiiy o?aaeaiiy,
i?ioeaea?aaiio oa aioeoiene/io ae?th;

4) oe? i?e?ii?aai?cie oaoiieia?/i? o ae?iaieoeoa?, noaa?euei? i?e
caa??aaii?;

5) aiie aeieia?/ii aacia/i?.

Aaaeeeai, ui aaiaoe/i? iniaeeaino? aaoeee aeia?a aea/aii, ? oiio aiie ?
ia?niaeoeaiith nenoaiith aeey eeiioaaiiy /oaei??aeieo aai?a. O iao /an
iaoiaeaie aaiaoe/ii? ?iaeaia??? ia iniia? aa?iaieo nii?iooai?thth/eo
aaeoa??e iaea?aeaii noia?i?iaeooeaioe a?ieia?/ii aeoeaieo ?a/iaei. Oea
a?aee?eaa? ia?niaeoeao eiino?othaaiiy ooai?a aaoeee c caieaiiaaieie
aeanoeainoyie aeey aeei?enoaiiy ?o o neeaae? iiaeo i?ia?ioee?a.

Oaeei /eiii, aa?iai? nii?iooai?thth/? aaeoa??? ?iaeo Bacillus ?
ia?niaeoeaiith a?oiith aeey ?ic?iaee ia ?o iniia? i?eioeeiiai iiaeo
aenieiaoaeoeaieo a?ii?aia?ao?a, o oiio /ene? c caaeaieie aeanoeainoyie
iaoiaeaie aaiaoe/ii? ?iaeaia???.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie,oaiaie.

?iaioo aeeiiaii ca?aeii c ieaiaie iaoeiai-aeine?aeieo ?ia?o ?inoeoooo
i?e?ia?ieia?? ? a??onieia?? ?i.Ae.E.Caaieioiiai IAI Oe?a?ie, a oaeiae
i?ia?aiaie AeEIO (i?iaeo 01.11.09/001-92 “?ic?iaeoe i?aia?ao a?inii?ei,
i?ecia/aiee aeey i?io?eaeoeee oa e?eoaaiiy o ethaeae aeenaaeoa??ic?a ?
aino?eo oeoieiai-eeoeiaeo caoai?thaaiue, iaoiiaeaieo iaoiaaiieie oa
oiiaii iaoiaaiieie i?e?ii?aai?ciaie”; i?iaeo 01.02.02/018-92 “?ic?iaeoe
iiaee aeieia?/ii /enoee a?ii?aia?ao c aioeaaeoa??aeueiith oa
aioea??oniith aeoeai?noth”; i?iaeo 05.02/106 “Aea/aiiy ?aaoeyoe??
neioaco aiaeiaaiiiai ?ioa?oa?iio i?ae aieeaii B.subtilis a ee?oeiao
??cieo nenoai i?aai?cio oaieie?iaieo”), AeIOI “?ioiii?io?eaeoeea
ianaeaiiy” (i?iaeo 11 “?ic?iaea iaoiae?a i?aeaeuaiiy aoaeoeaiino?
canoinoaaiiy ?ioiia?ieia?/ieo i?aia?ao?a oa ai?iaaaeaeaiiy ?o o i?aeoeeo
ioi?iie caei?ia’y”), I?iiaoee Oe?a?ie (i?iaeo 02.02/5179 “Ai?iaaaeeoe a
iaaee/io i?aeoeeo i?eioeeiiai iiaee i?ia?ioee noaae?i eiiieaenii?
ae??”).

Iaoa ? caaea/? aeine?aeaeaiiy.

Iaoith ?iaioe aoei noai?aiiy ia iniia? aa?iaieo nii?iooai?thth/eo
aaeoa??e ?iaeo Bacillus i?eioeeiiai iiaeo i?ia?ioee?a oa ai?iaaaeaeaiiy
?o a iaaee/io oa aaoa?eia?io i?aeoeeo.

O a?aeiia?aeiino? aei iinoaaeaii? iaoe aoei iaiao?aeii ?ica’ycaoe oae?
caaea/?:

1) anaa?/ii aea/eoe a?ieia?/i? aeanoeaino? ooai?a aaoeee, ia?niaeoeaieo
aeey aeeth/aiiy aei neeaaeo i?ia?ioeea;

2) aeine?aeeoe aacia/i?noue a?ae?a?aieo aaeoa??aeueieo eoeueoo? ?
?ic?iaeaiiai a?ii?aia?aoo;

3) i?iaanoe ee?i?/ia aea/aiiy aoaeoeaiino? i?aia?aoo;

4) ?ic?iaeoe oaoiieia?th ae?iaieoeoaa i?aia?aoo oa iaoiaee eiai
eiio?ieth ;

5) i?iaanoe ii??aiyeueia aea/aiiy iiaiai i?aia?aoo oa eiia?oe?eieo
i?ia?ioee?a ia iniia? aaoeee;

6) iaoiaeaie aaiaoe/ii? ?iaeaia??? neiino?othaaoe ooai B.subtilis, yeee
i?iaeoeo? -2 ?ioa?oa?ii ethaeeie, ? ia eiai iniia? ?ic?iaeoe i?ia?ioee c
aioeaaeoa??aeueieie oa aioea??onieie aeanoeainoyie;

7) aea/eoe aeaye? aniaeoe iaoaiecio ae?? i?ia?ioee?a c aaoeee;

8) ?ic?iaeoe ii?iaoeaio aeieoiaioaoe?th ia i?aia?aoe oa ai?iaaaeeoe
i?ia?ioeee a i?aeoeeo.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a.

-Oai?aoe/ii iaa?oioiaaii oa aenia?eiaioaeueii aeiaaaeaii aeioe?euei?noue
noai?aiiy iiaeo i?ia?ioee?a ia iniia? aa?iaieo nii?iooai?thth/eo
aaeoa??e ?iaeo Bacillus.

-?ic?iaeaii iaoeia? canaaee eiino?othaaiiy ?aeiia?iaioieo aaoeee c
caieaiiaaieie aeanoeainoyie.

-Aia?oa ia iniia? aa?iaieo nii?iooai?thth/eo aaeoa??e ?iaeo Bacillus
?ic?iaeaii i?eioeeiiai iiaee i?ia?ioee a?inii?ei.

-Iieacaii, ui a?inii?ei oa?aeoa?eco?oueny oi?eaeueiei niaeo?ii
aioaaii?noe/ii? aeoeaiino?: a?i aoaeoeaiee i?ioe oaeeo i?e?ii?aai?ci?a
(Candida spp., Campylobacter spp., Yersinia spp.), ia ye? ia ae?thoue
?io? i?ia?ioeee.

-Aenia?eiaioaeueii iaa?oioiaaii i?eioeeie aeni?an-ae?aaiinoeee
ni?aaaeiino? eoeueoo? B.subtilis 3 ? B.licheniformis 31, ui
neeaaeathoue iniiao a?inii?eio.

-Aia?oa iaoiaeaie aaiaoe/ii? ?iaeaia??? neiino?oeiaaii ?aeiia?iaioiee
i?ia?ioee noaae?i, yeee oa?aeoa?eco?oueny aioeaaeoa??aeueiith oa
aioea??oniith aeoeai?noth.

-Iieacaii aoaeoeai?noue ia?i?aeueiiai canoinoaaiiy noaae?io i?e
aenia?eiaioaeueieo a??onieo ?ioaeoe?yo.

-Anoaiiaeaii cia/aiiy o?aineieaoe?? aaeoa??e-i?ia?ioee?a aeey
noeioeyoe?? ?ioiii? ?acenoaioiino? iae?ii?aai?cio.

-Aeyaeaii noeioeyoe?th niaoeeo?/ii? ?ioiii? a?aeiia?ae? i?e
ia?i?aeueiiio canoinoaaii? ?ic?iaeaieo i?ia?ioee?a i?ae /an
aaeoeeiaoe??.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a.

O iaaee/io i?aeoeeo ai?iaaaeaeaii i?eioeeiiai iiaee i?ia?ioee a?inii?ei
(iaoaio Oe?a?ie ? 689 ? iaoaio ?in?enueei? Oaaea?aoe?? ? 1722502).
A?inii?ei a?ae??ciy?oueny a?ae ?ioeo ca?oa?aeieo eiia?oe?eieo
i?ia?ioee?a, ye? aacothoueny ia aa?iaieo nii?iooai?thth/eo aaeoa??yo
(oea?aia?iaai, aaeoenoaoee, aioa?iaa?i?i). I?e ii??aiyeueiiio aea/aii?
a?inii?eio oa oeeo i?aia?ao?a anoaiiaeaii, ui a?inii?ei
oa?aeoa?eco?oueny oi?eaeueiei niaeo?ii aioaaii?noe/ii? aeoeaiino? ? a
oie aea /an ? iaeaacia/i?oei na?aae ieo.

I?e aea/aii? ee?i?/ii? aoaeoeaiino? a?inii?eio a iaaee/ieo caeeaaeao
Ee?aa, Aei?i?iiao?ianueea, Iineae ia ??cieo eiioeiaaioao oai?eo iieacaii
aacia/i?noue a?inii?eio, eiai aenieo e?eoaaeueio aoaeoeai?noue.
Anoaiiaeaii iiaio ae?i?iaoe?th naeueiiiae oa oeaae o an?o oai?eo, cia/ia
cieaeaiiy ??aaiy /e iiaia ae?i?iaoe?y oiiaii iaoiaaiieo aioa?iaaeoa??e,
aeeth/ath/e iaoiaaiio eeoeiao iaee/eo (o 73,9 e 64% oai?eo,
a?aeiia?aeii), iaoiaaiieo noao?eieie?a (o 75% oai?eo) oa a?ea?a ?iaeo
Candida (o 75% oai?eo).

Iaeacii I?i?noa?noaa ioi?iie caei?ia’y Oe?a?ie ? 219 a?ae 22.10.93 ?.
oa I?i?noa?noaa ioi?iie caei?ia’y ?in?enueei? Oaaea?aoe?? ? 353 a?ae
29.12.92 ?. a?inii?ei aeicaieaii aei canoinoaaiiy a iaaee/i?e
i?aeoeoe?.

A?inii?ei ca?a?no?iaaii a Oe?a?i? ( ? 12.22.10.93) oa a ?in?enuee?e
Oaaea?aoe?? ( ? 010094). Caoaa?aeaeaii Oa?iaeiiaei? noaoo? ia a?inii?ei
a Oe?a?i? (ON 42O- 200/8-142-97) ? a ?in?enuee?e Oaaea?aoe?? (ON
42-3476-98), ?ino?oeoe?? ii canoinoaaiith i?aia?aoo, ?aaeaiaioe
ae?iaieoeoaa a?inii?eio.

I?iieneiaee aeione a?inii?eio a Oe?a?i? i?iaiaeeoueny AAO “Aei?i?ioa?i”.
A 1993-98 ?.?. aeiouaii a?eueo ye 5 iei. aiioe i?aia?aoo. Ca oeae
ia??iae ia ca?a?no?iaaii aeiaaee?a i?iieneiaiai a?aeo, aeiaeii?
?aeeaiaoe?? ia caaiae ia iinooiaei.

Ca oeeee ?ia?o “Oai?aoe/ia iaa?oiooaaiiy, eiino?othaaiiy, inai?iiy
ae?iaie/iai aeioneo oa ai?iaaaeaeaiiy a ee?i?/io i?aeoeeo i?eioeeiiai
iiaiai iaaee/iiai i?ia?ioeea — a?inii?ei” i?enoaeaeaii Aea?aeaaio
i?ai?th Oe?a?ie a aaeoc? iaoee ? oaoi?ee ca 1995 ?. (aeeieii ? 4050).

E?oeaic?th ia a?inii?ei i?iaeaii a ?in?enueeo Oaaea?aoe?th ? i?iieneiaee
aeione a?inii?eio inai?iee Oeaio?ii iaoeiai-oaoi?/ieo i?iaeai
a?ieia?/iiai caoenoo IAe? i?e?ia?ieia?? II ?O (i.?eaoa?eiao?a).

O aaoa?eia?io i?aeoeeo ai?iaaaeaeaii iiaee ?aeiia?iaioiee i?ia?ioee
noaae?i, yeee ia ia? aiaeia?a o na?o? (iaoaioe ?in?enueei? Oaaea?aoe?? ?
1839459, 2035185). Noaae?i ?aeiiaiaeiaaii aeey i?io?eaeoeee oa e?eoaaiiy
oai?ia aaeoa??aeueii? oa a??onii? ao?ieia?? o oaa?ei (?a?no?aoe?eiee ?
IA?1.03.0728-98). Caoaa?aeaeaii oaoi?/i? oiiae ia noaae?i, ianoaiiao ii
canoinoaaiith i?aia?aoo o aaoa?eia???. Ae?iaieoeoai noaae?io
cae?enith?oueny AAO “Aei?i?ioa?i”.

A Oe?a?i? cae?i/othoueny ee?i?/i? aei?iaoaaiiy aoaeoeaiino? noaae?io,
ye? i?iaiaeeeeny a?aeiia?aeii aei ??oaiiy Eii?oaoo c ieoaiue
?ioiia?ieia?/ieo i?aia?ao?a IIC Oe?a?ie ca?aeii c caoaa?aeaeaiith
i?ia?aiith.

Iniaenoee aianie caeiaoaa/a.

Aaoi?o iaeaaeeoue: ?ic?iaea eiioeaioe?? oa iai?yieo aeine?aeaeaiue,
aaciina?aaei? i?iaaaeaiiy aenia?eiaioaeueieo aeine?aeaeaiue, aiae?c ?
ocaaaeueiaiiy iaea?aeaieo aeaieo, oi?ioethaaiiy aeniiae?a. O ?iaio?
aeei?enoai? iaoa??aee, a iniiaiiio io?eiai? iniaenoi aaoi?ii, a oaeiae o
ni?aaaoi?noa?.

Ie?ai? aeine?aeaeaiiy aoei i?iaaaeaii ?acii c N.?.?acieeii (aeaye? aoaie
?ic?iaee a?inii?eio); A.I.Aaeyanueeith (eiino?othaaiiy ?aeiia?iaioiiai
ooaia B.subtilis); A.I. E?aia?iaei (aea/aiiy naeo-niaoeeo?/ieo
aiaeiioeeaac aaeoa??e ?iaeo Bacillus); N.E.?eaaeei, A.A.Oai??i (aea/aiiy
aioea??onii? aeoeaiino? i?ia?ioee?a, oanooaaiiy ?ioa?oa?iio),
A.A.?oaeaiei (aea/aiiy /ooeeaino? aaoeee aei aioea?ioee?a),
A.I.I?eiaith (iaea?aeaiiy ethi?ianoeaioieo ne?iaaoie), I.I.A?a/iaith,
A.I.Aeiia?aaeiaei, A.A.Oioaiii (i?iaaaeaiiy ee?i?/ieo aei?iaoaaiue
a?inii?eio). An?i ni?aaaoi?ai aeneiaethth aeeaieo iiaeyeo.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??.

Iniiai? ?acoeueoaoe i?iaaaeaieo aeine?aeaeaiue aoee i?aaenoaaeai? ia:
VI ? VII c’?caeao Oe?a?inueeiai i?e?ia?ieia?/iiai oiaa?enoaa
(Aeiiaoeuee, 1984; *a?i?aoe?,1989); VII c’?cae? Ananithciiai
i?e?ia?ieia?/iiai oiaa?enoaa (Aeia-Aoa, 1985); XI Oe?a?inueeiio
?anioae?eainueeiio c’?cae? i?e?ia?ieia?a, ai?aeai?ieia?a, ia?aceoieia?a
(Iaeana, 1985); ananithcieo eiioa?aioe?yo “Aeineioac aoi?e/iuo
iaoaaieeoia” (Ioueii, 1987), “Eieiiecaoeeiiiay ?acenoaioiinoue e
oeieioa?aiaaoe/aneea aioeaaeoa?eaeueiua i?aia?aou” (Iineaa, 1988),
“Aeineioac oa?iaioia iee?ii?aaieciaie” (Oaoeaio, 1988); “Iieaeoey?iua
iaoaieciu aaiaoe/aneeo i?ioeannia” (Iineaa, 1990); “Iee?iaeieiae/aneea e
aeioaoiieiae/aneea iniiau eioaineoeeaoeee ?anoaieaaiaenoaa e
ei?iii?iecaiaenoaa” (Aeia-Aoa, 1990); “Aeieiae/aneea iniiau aunieie
i?iaeoeoeaiinoe naeueneioicyenoaaiiuo aeeaioiuo” (Ai?ianuee, 1990);
ana?in?enueeeo eiioa?aioe?yo “Iiaua iai?aaeaiey aeioaoiieiaee” (Ioueii,
1994); “Aeenaaeoa?eicu e yoaeioeee” (Iineaa, 1996); IV ?in?eneiio
iaoe?iiaeueiiio eiia?an? “*aeiaae e eaea?noai” (Iineaa, 1997);
iaoeiai-i?aeoe/i?e eiioa?aioe?? “Ia?niaeoeau eniieueciaaiey yoaeioeea
“Aeinii?ei” a i?aeoeea cae?aaiio?aiaiey e aiaiii-iaaeeoeeineie neoaeau”
(?eaoa?eiao?a, 1997); i?aeia?iaei?e eiioa?aioe??,

i?enay/ai?e iai’yo? aeaaeai?ea A.A.Aa?aa (Iineaa, 1996); I?aeia?iaeieo
eiia?anao “Bacteriology & Mycology” (Inaea, ssiii?y, 1990); “Industrial
Use of Enzymes” (I?ca, ?oae?y 1990); “Genetics of Industrial
Microorganisms” (No?anao?a, O?aioe?y, 1990); VII I?aeia?iaeiiio eiia?an?
c ?ioaeoe?eieo oai?ia (Aiieiia, 1996); VI I?aeia?iaei?e eiioa?aioe?? c
iaaeeoeeie (Aeaioeueei, Iaeneea, 1995); IX I?aeaeoia?iaei?e eiioa?aioe??
ii aaoeeeai (Eicaiia, Oaaeoea??y, 1997).

Ioae?eaoe??.

?acoeueoaoe aeena?oaoe?? aeeeaaeaii o 63 iaoeiaeo i?aoeyo, na?aae yeeo
1 iaoiaee/i? ?aeiiaiaeaoe??, 20 noaoae a iaoeiaeo aeo?iaeao, 30 oac o
ca??ieeao eiioa?aioe?e, 9 iaoaio?a, 3 iiceoeaieo ??oaiiy i?i aeaea/o
iaoaio?a.

No?oeoo?a ? ianya aeena?oaoe??.

Aeena?oaoe?y neeaaea?oueny c? anooio, 11 ?icae?e?a iniiaii? /anoeie,
ocaaaeueiaiiy ?acoeueoao?a aeine?aeaeaiue, aeniiae?a, nieneo
aeei?enoaieo e?oa?aoo?ieo aeaea?ae.

?iaioo aeeeaaeaii ia 377 noi??ieao, ?ethno?iaaii 49 oaaeeoeyie, 42
iaethieaie (52 ia ie?aieo a?eooao). Nienie aeei?enoaii? e?oa?aoo?e
aeeth/a? 520 aeaea?ae, o oiio /ene? 337- ?iicaiieie iiaaie.

INIIAIEE CI?NO

A o?ueio ?icae?eao iaeyaeo e?oa?aoo?e i?aaenoaaeaii oa?aeoa?enoeeo
no/aniiai noaio i?iaeaie i?ia?ioee?a ? oai?aoe/ii iaa?oioiaaii i?aeoiaee
aei eiino?othaaiiy i?ia?ioee?a ia iniia? aaeoa??e ?iaeo Bacillus.

?icae?e 4. Iaoa??aee ? iaoiaee aeine?aeaeaiue.

Ia’?eoaie aeine?aeaeaiue aoee 215 ooai?a aa?iaieo nii?iooai?thth/eo
aaeoa??e ?iaeo Bacillus c iocath ?inoeoooo i?e?ia?ieia?? ? a??onieia??
IAI Oe?a?ie, a oaeiae aeecueei 200 ooai?a i?e?ii?aai?ci?a ?ioeo
oaeniiii?/ieo a?oi. Aenia?eiaioaeuei? aeine?aeaeaiiy i?iaaaeaii ia
eaai?aoi?ieo oaa?eiao (o oiio /ene? ?ia?aaeieo), iaaiao iaeaeao-?acon,
n?eueneiainiiaea?nueeeo oaa?eiao.

O ?iaio? aeei?enoiaoaaee:

— aeeaioi?ni? eoeueoo?e a??on?a a?eio A/A/1/68(I3N2); A/PR8(HoN1);

— oeaieii? eoeueoo?e a??on?a: aa?iano I aioeaaiiiai oeio (ooai ON) ?
II aioeaaiiiai oeio (ooai MS) ; aioeaoaeii??e?oo eiiae; aaceeoey?iiai
noiiaoeoo (ooai ?iae?aia).

Aoaeiii? ooaie iaea?aeoaaee c eieaeoe?? a??on?a ?inoeoooo a??onieia??
?i.Ae.?.?aaiianueeiai AII ?O, a??on aioeaoaeii??e?oo eiiae — c eieaeoe??
eoeueoo? AeIOe AA “Aaeoi?”.

Eoeueoo?e ee?oei:

— aeeiei?aei? ee?oeie o?a?iaeano?a ethaeeie (AeOE AeE-58);

— ia?aaeai? eoeueoo?e o?a?iaeano?a ieo? (L-929);

— ia?aaeai? eoeueoo?e ee?oei ie?ee naei? (NIAA)

Ieaci?aee: pPL608 , ?AIA 104 , ?AIA 105, pBR322, pUC19 — iaea?aeai? c
AeIOe AA “Aaeoi?” oa ?inoeoooo a?eea AI N?N?.

*ooeea?noue aaoeee aei aioei?e?iaieo i?aia?ao?a aea/aee
aeenei-aeeooc?eiei iaoiaeii (Iaiueoeeia, 1987).

Aeeiai?eo ?inoo i?e?ii?aai?ci?a o i?enooiino? aioei?e?iaieo i?aia?ao?a
aea/aee ca aeiiiiiaith oaeaee?nii? aaoiiaoeciaaii? nenoaie aeey
i?e?ia?ieia?/ieo ? iaoeiaeo aeine?aeaeaiue MS-2 o??ie “Abbott”.

Aea/aiiy i?e?ia?ioeaiico eeoa/ieea i?iaiaeeee ca?aeii c iaoiaee/ieie
?aeiiaiaeaoe?yie (A?a/aaa e ae?., 1986).

I?e i/euaii? naeo-niaoeeo?/ieo aiaeiioeeaac (NNA) aeei?enoiaoaaee
oinoioeaetheico ?-II (“Whatman”, NOA); ieneaiaoeo, yeee neioacoaaee
ca?aeii c iienaiith iaoiaeeeith (Mazin,1974); aieoao naoa?ico ? aaia?ei
naoa?ico — aiooaaee ia iniia? naoa?ice CL6B (“Pharmacia”, Oaaoe?y)
ca?aeii c a?aeiieie iaoiaeaie (Bickle et al, 1977; Dean et al, 1979).

Niaoeeo?/i?noue NNA aecia/aee ca ?acoeueoaoaie a?ae?ie?co ieie ??cieo
AeIE c a?aeiiith ia?aeiiith no?oeoo?ith (pBR322, pUC19, oaa?a , I-13,
O174).

Iaea?aeaiiy eiiiaoaioieo ee?oei aaoeee ? o?ainoi?iaoe?th ieaci?aeiith
AeIE i?iaiaeeee ca iaoiaeii Spizizen (1958).

Ieaci?aeio AeIE ?c ooai?a aaoeee aeae?eyee noaiaea?oieie iaoiaeaie
eoaeiiai e?ceno (Aeiaa?, 1988) oa iiaeeo?eiaaieie iaie iaoiaeaie.

Iayai?noue eaeeioeeoa?iiai -2 ?ioa?oa?iio ethaeeie a eoeueoo?aeueiiio
na?aaeiaeu? ?aeiia?iaioiiai ooaio B.subtilis 3 (105) aecia/aee i?yiei
?ioiioa?iaioiei aiae?cii ?c canoinoaaiiyi eiia?oe?eiiai iaai?o
“Tween-Test” (Naieo-Iaoa?ao?a), a oaeiae ca a?ieia?/iith aeoeai?noth
(aaeueioaaiiyi oeeoiiaoe/ii? ae?? a??ono aaceeoey?iiai noiiaoeoo).

Aeey aecia/aiiy noaa?eueiino? ieaci?aeii? AeIE o ee?oeiao aaoeee
eoeueoo?e ae?iuoaaee ia iiaeeaieo na?aaeiaeuao aac naeaeoeaiiai oeneo, a
oaeiae ia?aaeaaee /a?ac i?aai?ci eaai?aoi?ieo oaa?ei.

No?oeoo?io noaa?euei?noue ieaci?ae (cie?aia caa?aaeaiiy aaia ?ioa?oa?iio
oeio -2) aea/aee iaoiaeii a?a?eaeecaoe?? (Iaieaoen e ae?., 1984).

Aeaeo?ioi?ac o iie?ae?eeai?aeiiio aae? a i?enooiino? aeiaeaoeeenoeueoaoo
iao??th i?iaiaeeee ca iaoiaeii Laemmli (1970).

Aeiee?i?/ia aea/aiiy i?ia?ioee?a i?iaiaeeee ca?aeii c ?Ae
42-28-8-89.

I?e aecia/aii? aacia/iino? aaeoa??aeueieo eoeueoo? oa i?ia?ioee?a ia ?o
iniia? i?iaiaeeee a?noieia?/ia aea/aiiy i?aai?a eaai?aoi?ieo oa
n?euenueeiainiiaea?nueeeo oaa?ei.

Niaoeeo?/i?noue ?ioiieo ethi?ianoeaioieo ne?iaaoie aea/aee ii
a?aeiioaiith aei 78 ooai?a oanoe aeae?a aaoeee (B.subtilis, B.
licheniformis, B. cereus, B. pumilus, B. coagulans, B. macerans), na?aae
yeeo aoee iocaei? eoeueoo?e, a oaeiae ooaie, ia iniia? yeeo
?ic?iaeythoueny iia? a?ii?aia?aoe.

Iae?ioaae ia?eoiiaaeueiiai aenoaeaoo iaea?aeoaaee ca iienaiith
iaoiaeeeith (O/eoaeue, 1978). Ooieoe?iiaeueio aeoeai?noue iae?ioaa?a
aecia/aee iaoiaeii ethi?iiecaeaaeii? oai?ethi?ianoeaioe?? (Aeaaeeie?ia e
ae?.,1989).

Aioea??oni? aeanoeaino? i?ia?ioee?a aea/aee ia iiaeaeyo
aenia?eiaioaeueii? aa?iana??onii? ? a?eiicii? ?ioaeoe?? ieoae,
aai?oaeueiiai aa?iano ii?nueeeo naeiie (Ia?aiieeiaa e ae?., 1986) oa
iniaeeai iaaacia/ii? a??onii? ?ioaeoe??, iaoiiaeaii? a??onii
aioeaoaeii??e?oo eiiae o ii?nueeeo naeiie.

Aenia?eiaioaeueio eaii?eiaaeoa??icio ?ioaeoe?th a?aeoai?thaaee ca
?ic?iaeaiith iaie iaoiaeeeith (Sorokulova et al., 1997).

Aeey aea/aiiy o?aineieaoe?? aaeoa??e oaa?eiai aaiaeeee ia?i?aeueii ii
iaei?e aeic? a?ii?aia?ao?a (eie?aaeoa?eio, eaeoiaaeoa?eio,
a?o?aeoiaaeoa?eio, a?inii?eio, noaae?io, aaeoenoaoeeo) aai /eno?
eoeueoo?e aaeoa??e, ye? neeaaeathoue iniiao oeeo i?aia?ao?a, o aeic?
a?ae 103 aei 1010 i?e?iaieo ee?oei.

Acyooy e?ia? a ieoae aeey aaeoa??ieia?/iiai aeine?aeaeaiiy i?iaiaeeee c
i?aeeeth/e/ii? aaie i?e aaoaiac?? aai i?eaeeoo?ai — c ?ao?ii?a?oaeueiiai
aaiiciiai nieao?iiy. I?iae e?ia? aen?aaee ia aeaioacia na?aaeiaeua
(sseiaeaa, E?aniiiaaoeaaa, 1964) oa ia a?aeiia?aeia aaa?eciaaia
n?aaeiaeua.

Aiiiaaiaoe aioo??oi?o i?aai?a, iaea?aeai? anaioe/ii, aen?aaee ia
aaa?eciaai? iiaeeai? na?aaeiaeua. E?euee?niee i?ae?aooiie i?iaiaeeee ca
Gould (1965) aai aen?aii iine?aeiaieo aeanyoee?aoieo ?icaaaeaiue
aiiiaaiao?a.

Aenia?eiaioaeuei? aeai? ia?iaeyee noaoenoe/ii (Aoia?ei, Ai?iaueaa,
1962).

?icae?e 5. noai?aiiy oa aenia?eiaioaeueia aea/aiiy a?inii?eio

Aeoiaey/e c ?acoeueoao?a aaaaoi??/iiai aea/aiiy a?ieia?? aa?iaieo

nii?iooai?thth/eo aaeoa??e ?iaeo Bacillus, a oaeiae aacoth/enue ia
oai?aoe/ieo ia?aaeoiiaao aei i?aeai?o ooai?a, ia?niaeoeaieo aeey
aeeth/aiiy aei neeaaeo a?ii?aia?ao?a, a?ae?a?aee aea? eoeueoo?e —
B.subtilis 3 oa B.licheniformis 31,- ye iniiao iiaiai i?ia?ioeea —
a?inii?eio. Oe? eoeueoo?e oa?aeoa?ecothoueny oeiiaeie aeey aeaieo aeae?a
o?c?ieiai-a?io?i?/ieie iciaeaie, ye? iiaeooue iaoiiaeoe oe?oee ae?aiacii
?o ae?? i?e noi?niiio aeei?enoaii?. Oae, B. licheniformis 31, ia
a?aei?io a?ae B. subtilis 3, oa?aeoa?eco?oueny caeaoi?noth ?inoe a
aiaa?iaieo oiiaao, ui ? iaeiei c iieaciee?a ??cii? aaeaioaoe?eii?
iiaeeeaino? eoeueoo? o ??cieo a?aeae?eao oeoieiai-eeoeiaiai o?aeoo.
A?ae??ciythoueny oe? eoeueoo?e oaeiae ? ca ?ioeie aeanoeainoyie:
/ooeea?noth aei aioei?e?iaieo i?aia?ao?a, niaeo?ii aioaaii?noe/ii?
aeoeaiino?. Iieacieee niaoeeo?/ii? aeoeaiino? B.subtilis 3 ? B.
licheniformis 31 uiaei S.sonnei, S.flexneri, S.typhi, S.typhimurium, S.
aureus, C. albicans noaoenoe/ii aeinoia??ii a?ae??ciythoueny

Aenia?eiaioaeueieie aeine?aeaeaiiyie anoaiiaeaii, ui eoeueoo?e a
neeaae? i?aia?aoo iathoue aooe i?aaenoaaeai? a oaeiio e?euee?niiio
ni?aa?aeiioaii?: B.subtilis 3 — 1.109-8.109; B.licheniformis 31 — 0,1
. 109 — 2 . 109.

I?e niieo/aii? oeeo aeaio eoeueoo? o neeaae? a?ii?aia?aoo noia?ia
aioaaii?noe/ia aeoeai?noue a?eueo ae?aaeaia aei oe?ieiai niaeo?o
iaoiaaiieo oa oiiaii iaoiaaiieo i?e?ii?aai?ci?a-caoaeiee?a
oeoieiai-eeoeiaeo ?ioaeoe?e (oaae. 1).

Iniaeeai? oaaae caneoaiao? aecia/aia aeniea aioaaii?noe/ia aeoeai?noue
a?inii?eio uiaei eaii?eiaaeoa??a, e??nei?e, a?ea?a ?iaeo Candida. Aein?
iaa?aeii? a?ii?aia?aoe, ye? oa?aeoa?ecothoueny aioaaii?noe/iith ae??th
i?ioe oeeo i?e?ii?aai?ci?a. Aaaeeeai oaeiae a?aecia/eoe, ui a?inii?ei
ia i?eai?/o? i?e?ii?aai?ci?a-i?aaenoaaiee?a ii?iaeueii? i?e?ioei?e.

Aea/aiiy aacia/iino? eoeueoo?, ui aoiaeyoue aei neeaaeo a?inii?eio, oa
eaai?aoi?ieo na??e i?aia?aoo i?iaiaeeee ia eaai?aoi?ieo oaa?eiao ca?aeii
c ?Ae 42-28-8-89, a oaeiae ia iaaiao iaeaeao-?acon.

I?e aea/aii? aino?i? oiene/iino? aoei anoaiiaeaii, ui aaaaeaiiy oaa?eiai
eoeueoo?, ye? neeaaeathoue iniiao a?inii?eio, ? aaaaeaiiy naiiai
i?aia?aoo ??cieie niiniaaie (oaeiae ? ia?aioa?aeueii) a aeicao, ui
cia/ii ia?aaeuothoue ?aeiiaiaeiaai? aeey aaeeaaiiy, o oaa?ei ia
niinoa??aa?oueny aoaeue-yeeo iaoieia?/ieo ci?i, ui aaccanoa?aaeii
aeiaiaeyoue aeai? a?noieia?/ieo aeine?aeaeaiue. Aiaeia?/i? ?acoeueoaoe
aacia/iino? a?inii?eio aeyaeaii i?e aea/aii? o?ii?/ii? oiene/iino?.

?acoeueoaoe, io?eiai? ia eaai?aoi?ieo oaa?eiao, i?aeoaa?aeaeai? aea-

ieie aei?iaoaaiiy i?aia?aoo ia i?eiaoao — iaeaeao-?acon. Aaaaeaiiy
a?inii?eio oaa?eiai i?ioyaii 10 aei?a aea?/? ia aeaiue ia aeeeeeaei
i?yeeo neiioii?a ii?ooaiue a i?aai?ci? iaai. O ii?i? caeeoaeeny an?
ee?i?/i? ? aaeoa??ieia?/i? iieacieee.

?icae?e 6. Ee?i?/ia aea/aiiy a?inii?eio

?aaeoiaaii?noue ? aacia/i?noue i?aia?aoo aea/aee ia aeia?iaieueoeyo —
caei?iaeo ethaeyo a?eii a?ae 25 aei 57 ?ie?a, na?aae yeeo aoei 11 ae?iie
? 10 /ieia?e?a. An? aeia?iaieueoe? ciaoiaeeeeny i?ae nenoaiaoe/iei
e?ea?nueeei iaaeyaeii ia iaio ye 2 i?nyoe? a?ae ii/aoeo i?eeiio
i?aia?aoo. Aoee i?iaaaeai? ee?i?/i? ianoaaeaiiy, a oaeiae
aaeoa??ieia?/i? aeine?aeaeaiiy ca?aeii c i?ia?aiith aei?iaoaaiue. Ca
aanue ia??iae niinoa?aaeaiiy a aeiaeiiio aeiaaeeo ia iii?/aii iieacaiue
aei aeino?ieiaiai i?eieiaiiy i?eeiio i?aia?aoo. An? iniiai? ee?i?/i? oa
aaeoa??ieia?/i? iieacieee caeeoaeeny a iaaeao ii?ie. Oaeei /eiii,
i?iaaaeai? aeine?aeaeaiiy aeiaaee aacia/i?noue a?inii?eio aeey
aeia?iaieueoe?a.

Aea?aeaai? aei?iaoaaiiy i?aia?aoo aoei i?iaaaeaii o a?aeiia?aeiino? aei
caoaa?aeaeaii? i?ia?aie ca?aeii c Iaeacii I?i?noa?noaa ioi?iie caei?ia’y
N?N? ? 107-O io 20.07.91 a. Aieiaiith iaoith ? caaaeaiiyi aei?iaoaaiue
aoei aea/aiiy ee?i?/ii? aoaeoeaiino? a?inii?eio ye caniao i?io?eaeoeee ?
e?eoaaiiy aeenaaeoa??ic?a oa aino?eo oeoieiai-eeoeiaeo ?ioaeoe?e.

Aeey aea?aeaaieo aei?iaoaaiue a?inii?ei aoei i?aaenoaaeaii o aeaeyae?
na??e c ai?noii a iaei?e aeic? 4 .105- 2. 106 oa 1. 109- 4,5. 109
aeeaeo i?e?iaieo ee?oei. sse i?aia?ao ii??aiyiiy aeei?enoiaoaaee
eaeoiaaeoa?ei.

Aea?aeaai? aei?iaoaaiiy a?inii?eio aoei i?iaaaeaii a
Aei?i?iiao?ianueeiio iaoeiai-aeine?aeiiio ?inoeooo? aano?iaioa?ieia??,
ee?i?/iiio a?aeae?e? Iineianueeiai iaoeiai-aeine?aeiiai ?inoeoooo
ai?aeai?ieia?? ? i?e?ia?ieia?? ?i.A.I.Aaa?e/aanueeiai, Ee?anueeiio
?inoeooo? oaeineiiaeaiiy e?ea??a aeey e?eoaaiiy aei?ineeo ? ae?oae c
aino?eie eeoeiaeie ?ioaeoe?yie oa o?ii?/ieie caoai?thaaiiyie i?aai?a
o?aaeaiiy, oneeaaeiaieo aeenaaeoa??icaie.

I?e e?eoaaii? aino?eo eeoeiaeo ?ioaeoe?e ca an?ia ee?i?/ieie ? iniaeeai
ca aaeoa??ieia?/ieie iieacieeaie iaee?au? ?acoeueoaoe io?eiaii aeey
a?inii?eio c aenieei ai?noii aeeaeo i?e?iaieo ee?oei a

Oaaeeoey 1

Niaoeeo?/ia aeoeai?noue i?aia?aoo a?inii?ei

Oano-eoeueoo?eE?euee?noue aeine?aeaeaieo ooai?aCiia ca-

o?eieeanueiaic ieo /ooeeaeo?inoo, iiBacillus anthracis
22228Campylobacter coli43171C. jejuni77194C. laridis100Escherichia coli
1818134Enterobacter sp. 22228Klebsiella pneumoniae 11102Klebsiella
sp.9983Proteus mirabilis44134Proteus vulgaris88214Pseudomonas
aeruginosa400Salmonella abortus-equi 11121S. derby 11162S. greiz 11195S.
newport 11111S. stanley 11151S. typhi 11202S. typhimurium 77122Serratia
marcescens 66155Shigella flexneri 22231S. sonnei 1313214Yersinia
enterocolitica 99182Y. pseudotuberculosis 1111201Candida albicans
2319303C. parapsilosis44282C. pseudotropicalis 2242C.
stellatoidea99304C. tropicalis54324Lactobacillus fermentum 600L.
plantarum 800Staphylococcus aureus 2525225S. epidermidis
22204Streptococcus faecium11121iaei?e aeic? (oaae.2). Aiaeia?/i?
?acoeueoaoe aeyaeaii i?e e?eoaaii? iaoe??io?a c o?ii?/ieie
caoai?thaaiiyie i?aai?a o?aaeaiiy, oneeaaeiaieo aeenaaeoa??icaie ??ciiai
nooiaith. sse ? i?e aino?eo eeoeiaeo ?ioaeoe?yo, ii?iae?caoe?y
i?e?ioei?e aoea iaeaoaeoeai?oa i?e canoinoaaii? a?inii?eio c aenieei
ai?noii aeeaeo i?e?iaieo ee?oei (oaae.3).

I?aenoiiaoth/e aeai?, io?eiai? a ??cieo ee?i?/ieo caeeaaeao, iiaeia
c?iaeoe aeniiaie, ui ia an?o aea/aieo eiioeiaaioao oai?eo a?aecia/aii
aacia/i?noue a?inii?eio, eiai aenieo ee?i?/io aoaeoeai?noue. Iaee?au?
ee?i?/i? oa aaeoa??ieia?/i? iieacieee anoaiiaeaii i?e canoinoaaii?
a?inii?eio c aenieei ai?noii aeeaeo i?e?iaieo ee?oei. Oae, a?aecia/aii
iiaio ae?i?iaoe?th naeueiiiae ? oeaae o an?o oai?eo, cia/ii cieaeoaaeany
e?euee?noue aai iiai?noth ae?i?ioaaeeny oiiaii iaoiaaii? aioa?iaaeoa???,
aeeth/ath/e iaoiaaiio eeoeiao iaee/eo (o 73,9 ? 64% oai?eo
a?aeiia?aeii), iaoiaaii? noao?eieiee (o 75% oai?eo) ? a?eae ?iaeo
Candida (o 75% oai?eo). Ee?i?/iee aoaeo i?e canoinoaaii? a?inii?eio c
iecueeei ai?noii aeeaeo i?e?iaieo ee?oei ia a?ae??ciyany a?ae aieeao
eaeoiaaeoa?eio ? aoa iaio ae?aaeaiee.

Aaaeeeaei ?acoeueoaoii i?iaaaeaieo ee?i?/ieo aei?iaoaaiue ? aeai? i?i
aoaeoeai?noue e?euee?niiai ai?noo aeeaeo i?e?iaieo ee?oei a iaei?e aeic?
i?aia?aoo. Anoaiiaeaii, ui iaeiioeiaeuei?oa ?aciaa aeica ? 1.109 —
1.1010 aeeaeo i?e?iaieo ee?oei. ?acoeueoaoe ee?i?/ieo aei?iaoaaiue
i?aeoaa?aeeee aeai?, io?eiai? in vitro, ye? oaeiae iaa?oioiaothoue oe?
e?euee?ni? oa?aeoa?enoeee a?inii?eio.

?icae?e 7. ii??aiyeueia aea/aiiy a?inii?eio oa ?ioeo eiia?oe?eieo
i?aia?ao?a ia iniia? aaoeee

O iaaee/i?e i?aeoeoe? ??cieo e?a?i canoiniaothoue i?aia?aoe ia iniia?
aaeoa??e ?iaeo Bacillus. Oae, oe?ieiai ?iciianthaeaeaiiy a e?a?iao
Cao?aeii? ?a?iie iaaoa i?ia?ioee aaeoenoaoee ae?iaieoeoaa o??ie
“Galenika” (THaineaa?y) ? o?aioeocuenueei? o??ie “Marion Merrell“
(Tihole, 1982). A iniia? oeueiai i?aia?aoo — ooai B. cereus IP 5832 c
eieaeoe?? ?inoeoooo Ianoa?a (Ia?eae). A?aeiiee oaeiae a?ii?aia?ao
oea?aia?iaai (“Xing Jian”, Eeoae), ae?y yeiai iaoiiaeaia ooaiii B.
cereus DM-423 (Wei, Kan, 1981). Iniiaith aioa?iaa?i?io (“Sanofi
Winthrop”, ?oae?y) ? eoeueoo?a B. subtilis (Coppi, 1985).

I?iaiaeeee ii??aiyeueia aea/aiiy ?ic?iaeaiiai i?ia?ioeea a?inii?eio oa
a?aeiieo eiia?oe?eieo a?ii?aia?ao?a. Iaei??th c iaeaaaeeea?oeo
oa?aeoa?enoee i?ia?ioee?a ? ?o aacia/i?noue oa niaoeeo?/ia aeoeai?noue.
I?e aea/aii? niaoeeo?/ii? aeoeaiino? iniaeeai? oaaae i?eae?ey

ee ae?? oeeo a?ii?aia?ao?a ia oano-eoeueoo?e, ?acenoaioi? aei
aioea?ioee?a, a oaeiae ia o? aeaee oiiaii iaoiaaiieo i?e?ii?aai?ci?a,
uiaei yeeo ?io? a?aeii? i?ia?ioeee ia aoaeoeai?.

Io?eiai? aeai? na?ae/aoue i?i oa, ui na?aae i?ia?ioee?a c aaoeee o?eueee
a?inii?ei oa?aeoa?eco?oueny aenieith aioaaii?noe/iith aeoeai?noth uiaei
ee?i?/ieo ?cieyo?a eaiaeeae, noao?eieie?a, eaii?eiaaeoa??e, e??n?i?e,
aioa?iiaoiaaieo Escherichia, a oiio /ene? ? ?acenoaioieo aei
aioea?ioee?a (oaae. 4). Oae, ooai E. coli 683, no?eeee iaeii/anii aei
naie aioea?ioee?a, /ooeeaee aei ae?? a?inii?eio (ae?aiao? ciie cao?eiee
?inoo — 242 ii), a oie aea /an ?io? i?ia?ioeee c aaoeee eiai ia
i?eai?/othoue. Ooaie Campylobacter, ye? oa?aeoa?ecothoueny aenieith
?acenoaioi?noth aei aaaaoueio aioea?ioee?a, aoee /ooeeaeie aei
a?inii?eio. ?io? ia?aa??ai? a?ii?aia?aoe ia aeyaeee aioaaii?noe/ii? ae??
ia oe? eoeueoo?e.

Oaeei /eiii, iaea?aeai? aeai? na?ae/aoue i?i oa, ui ?ic?iaeaiee
i?ia?ioee a?inii?ei nooo?ai a?ae??ciy?oueny niaoeeo?/iith aeoeai?noth
a?ae a?aeiieo eiia?oe?eieo i?aia?ao?a c aaoeee.

I?iaiaeeee oaeiae ii??aiyeueia aea/aiiy aino?i? oiene/iino? i?ia?ioee?a
c aaoeee ye iaeiiai c iieaciee?a ?o aacia/iino?. Anoaiiaeaii, ui LD50
aeey a?inii?eio i?e aioo??oiuei/a?aaiiio aaaaeaii? ia?aaeuo? 1010 EOI.
Aeey aaeoenoaoeeo ? oea?aia?iaaio oe? iieacieee neeaaeathoue
a?aeiia?aeii 5.108 e 1.109 (oaae.4). I?iaaaeai? a?noieia?/i?
aeine?aeaeaiiy aioo??oi?o i?aai?a ieoae, ye? iaea?aeoaaee aaeoenoaoee a
aeicao, ?aeiiaiaeiaaieo aeey canoinoaaiiy ethaeyi, na?ae/eee i?i
ia?aianai? oieia? ?aaeoe??: ia??aiii??io iiaiie?iai?noue aioo??oi?o
i?aai?a,

aio?caio eaaai?a, ??ceo iiaiie?iai?noue ia/?iee, e?iaiaeeeae a
naeac?ioe?, ?oai?th ei?e ie?ie, aeeno?io?th i?iea?aea, aaiaoioeeo?a,
ia?aiaeo ee?oei ei?e aieiaiiai iiceo. Aeai?, iaea?aeai? aeey a?inii?eio,
na?ae/aoue i?i oa, ui aaaaeaiiy i?aia?aoo oaa?eiai iaa?oue o aeicao, ye?
cia/ii ia?aaeuothoue ?aeiiaiaeiaai? aeey canoinoaaiiy, ia aeeeeea? a ieo
aoaeue-yeeo iaoieia?/ieo ci?i, ui aeiaaaeaii ye i?e iae?ineii?/iiio
aea/aii?, oae ? i?e a?noieia?/ieo aeine?aeaeaiiyo.

Iaiao?aeii i?aee?aneeoe, ui a iniia? aaeoenoaoeeo ? oea?aia?iaaio —
ooaie B.cereus. Na?aae aaeoa??e ?iaeo Bacillus oea ?aeeiee aeae, yeee
a?aeianaii aei oiiaii iaoiaaiieo, ine?eueee /anoi a?i ? ao?ieia?/iei
oaeoi?ii oa?/iaeo oieeei?ioaeoe?e /a?ac i?iaeoeoe?th oiene/ieo
iaoaaie?o?a.

Oaeei /eiii, ca iniiaieie iieacieeaie a?inii?ei ia?aaa?oo? a?aeii?
i?ia?ioeee, ye? aacothoueny ia aaeoa??yo ?iaeo Bacillus.Oaaeeoey 4

Ii??aiyeueia oa?aeoa?enoeea i?ia?ioee?a c aaoeee

A?ii?aia?aoIniiaa i?aia?aooAino?a oiene/i?noue, LD50A?inii?eiB.subtilis
3 B.licheniformis 31> 1010 EOIAaeoenoaoeeB. cereus IP 58325 . 108 EOI
Oea?aia?iaaiB. cereus DM-4231 . 109 EOI

Niaoeeo?/ia aeoeai?noue i?ia?ioee?a c aaoeee

Oano-eoeueoo?eE?euee?noue aeine?ae-aeaieo ooai?a

E?euee?noue ooai?a, /ooeeaeo aei

i?ia?ioee?aA?inii-?eiAaeoe-noaoeeOea?aia?-iaaiAioa?iaa?-i?iCampylobacter
coli43000C. jejuni77000Candida albicans 2319000C. parapsilosis44000C.
pseudotropicalis 22000C. stellatoidea99000C.
tropicalis54000Staphylococcus aureus 2222000Yersinia
enterocolitica99000Y. pseudotuberculosis1111000

?icae?e 8. Oaoiieia?/i? i?eioeeie aeaioiaeaiiy a?inii?eio oa ?ic?iaea
iaoiae?a eiio?ieth i?aia?aoo

?ic?iaeai? aea? oaoiieia?? ae?iaieoeoaa a?inii?eio: iaeia c ieo —
iaoiaeii iiaa?oiaaiai ae?iuoaaiiy aaeoa??aeueieo eoeueoo? ia
aaa?eciaaieo iiaeeaieo na?aaeiaeuao, a ?ioa — c aeei?enoaiiyi aeeaeiiiai
eoeueoeaoaaiiy. Oaoiieia?/ia noaia iaeiaeiaa aeey iaio iaoiae?a,
ine?eueee aeeth/a? oae? iniiai? noaae??: iaea?aeaiiy iaoi/ieo eoeueoo?;
iaea?aeaiiy iaoeaii? a?iiane; i?eaiooaaiiy i?aia?aoo; e?io?e?caoe?y
i?aia?aoo; eiio?ieue aioiaiai i?aia?aoo ca?aeii c oa?iaeiiaeiith
noaooath; iaeoaaiiy oa ia?eoaaiiy i?aia?aoo.

A?inii?ei ae?iaey?oueny AAO “Aei?i?ioa?i” iaoiaeii iiaa?oiaaiai
eoeueoeaoaaiiy ? Oeaio?ii a?enueeiai-oaoi?/ieo i?iaeai a?ieia?/iiai
caoenoo IAe? i?e?ia?ieia?? II ?O — aeeaeiiei eoeueoeaoaaiiyi.

A?inii?ei, iaea?aeaiee ??cieie iaoiaeaie, ia a?ae??ciy?oueny ye ca
iniiaieie iieacieeaie yeino? ca?aeii c oa?iaeiiaeiith noaooath ia
i?aia?ao, oae ? ca ee?i?/iith aoaeoeai?noth.

Ii??aiyeuei? ee?i?/i? aei?iaoaaiiy e?eoaaeueii? ? i?io?eaeoe/ii?
aoaeoeaiino? a?inii?eio, aeaioiaeaiiai aeaiia iaoiaeaie, i?iaaaeaii
ca?aeii c caoaa?aeaeaiith i?ia?aiith. Iaea?aeai? ?acoeueoaoe
i?aeoaa?aeeee ?aeaioe/i?noue a?inii?eio, iaea?aeaiiai ??cieie iaoiaeaie,
ca oa?aeoa?ii ? nooiaiai ae?aaeaiino? e?eoaaeueiiai oa i?io?eaeoe/iiai
aoaeoo.

Aaaeeeaei iieacieeii o i?ioean? ae?iaieoeoaa ? i?e eiio?ie? a?ii?aia?aoo
? ia?aa??ea ni?aaaeiino? ooai?a, ye? aoiaeyoue aei eiai neeaaeo. Oiio
aeey caaacia/aiiy iaae?eiiai oa oaeaeeiai eiio?ieth ni?aaaeiino?
ae?iaie/eo ooai?a iaiao?aeii aoei ?ic?iaeoe iaoiaee aeni?an-ae?aaiinoeee
B. subtilis 3 ? B. licheniformis 31.

Iaie ?acii ?c ni?a?ia?oieeaie IAI “Aeioaoiieiaey” (i. Iineaa) io?eiaii
ae?aaiinoe/i? ne?iaaoee aeey ?ioiioeoi?anoeaioiiai aiae?co ae?iaie/eo
eoeueoo?. Niaoeeo?/i?noue ne?iaaoie ia?aa??aii ii a?aeiioaiith aei
eoeueoo? aa?iaieo nii?iooai?thth/eo aaeoa??e ?c eieaeoe?? ?inoeoooo
i?e?ia?ieia?? ? a??onieia?? IAI Oe?a?ie. Aea/aee iocaei? eoeueoo?e
??cieo aeae?a, a oaeiae ooaie, ia iniia? yeeo ?ic?iaeythoueny iia?
a?ii?aia?aoe. Anueiai aoei aea/aii 78 ooai?a oanoe aeae?a aaoeee
(B.subtilis, B. licheniformis, B. cereus, B. pumilus, B. coagulans, B.
macerans). O aeine?aeaeaieo ?icaaaeaiiyo (1:8 aeey ne?iaaoee aei
B.subtilis 3 ? 1:4 — aeey ne?iaaoee aei B. licheniformis 31) ia aeyaeaii
ia?ao?anieo aca?iiae?e oeeo ne?iaaoie c aaoa?ieia?/ieie eoeueoo?aie.
Niaoeeo?/i? ?aaeoe?? niinoa??aaee o?eueee c a?aeiia?aeieie aiiieia?/ieie
eoeueoo?aie.

Oaeei /eiii, iaea?aeai? ae?aaiinoe/i? ne?iaaoee aei ooai?a B. subtilis
3 ? B. licheniformis 31, ye? caaacia/othoue iaae?eio
aeni?an-ae?aaiinoeeo ni?aaaeiino? ae?iaie/eo eoeueoo? ia an?o
oaoiieia?/ieo aoaiao ae?iaieoeoaa a?inii?eio.

?icae?e 9. noai?aiiy i?eioeeiiai iiaeo aenieiaoaeoeaieo ?aeiia?iaioieo
i?ia?ioee?a ia iniia? aaoeee

A?inii?ei, ye ? an? ?io? a?aeii? i?ia?ioeee, ia ia? ae?aaeaii?
aioea??onii? ae??. O oie aea /an a?aeiii, ui a no?oeoo?? caoai?thaaiue
ethaeeie ? oaa?ei cia/io /anoeio neeaaeathoue ?ioaeoe?? a??onii? oa
a??onii-aaeoa??aeueii? ao?ieia??. Oiio ?ic?iaea aenieiaoaeoeaieo
i?aia?ao?a c aioeaaeoa??aeueieie oa aioea??onieie aeanoeainoyie ?
aeooaeueiith i?iaeaiith.

Iaeia?niaeoeai?oeie aeey noai?aiiy oaeeo i?aia?ao?a ? aa?iai?
nii?iooai?thth/? aaeoa???, oiio ui aiie oa?aeoa?ecothoueny ae?aaeaiith
aioaaii?noe/iith aeoeai?noth uiaei oe?ieiai niaeo?o iaoiaaiieo oa oiiaii
iaoiaaiieo i?e?ii?aai?ci?a. A?aeiii oaeiae, ui aaoeeee — c?o/ia ? aeia?a
aea/aia nenoaia aeey eeiioaaiiy /oaei??aeieo i?i- oa aoea??ioe/ieo
aai?a; ioaea ia ?o iniia? iiaeia eiino?othaaoe ooaie c caaeaieie
aeanoeainoyie.

Noai?aiiy ?aeiia?iaioieo i?ia?ioee?a iaaiai?th?oueny a e?oa?aoo?? ye
iaeia c aaaeeeaeo iai?yie?a aeine?aeaeaiue (Goldin, 1992; Tannock,1992;
Havenaar, 1992; Oaiaea?ia ,1997), aea aein? oae? i?ia?ioeee ia
?ic?iaeai?.

Aeoiaey/e c oeeo iineeaiue, aoei iinoaaeaii caaaeaiiy neiino?othaaoe
iaoiaeaie aaiaoe/ii? ?iaeaia??? ia iniia? aaoeee c aenieith
aioaaii?noe/iith aeoeai?noth uiaei iaoiaaiieo oa oiiaii iaoiaaiieo
i?e?ii?aai?ci?a iia? ooaie, ye? ii?yae c oeeie aaaeeeaeie aeanoeainoyie
iaee i?iyaeyoe aioea??onio aeoeai?noue caaaeyee aaaaeaiith aaiaoe/ii?
?ioi?iaoe??, ui eiaeo? i?iaeoeoe?th oi?aa?naeueii? aioea??onii? niieoee
— ?ioa?oa?iio.

Iaei??th c aaaeeeaeo oiia caaacia/aiiy oni?oii? o?ainoi?iaoe?? aaoeee
/oaei??aeiith AeIE ? a?aenooi?noue o ?aoeei??ioa nenoaie
?ano?eeoe??-iiaeeo?eaoe?? (Uaeeoiia, 1986). Oiio ia?oei aoaiii i?e
a?aeai?? ia?niaeoeaieo eoeueoo? aeey eeiioaaiiy AeIE aoei aea/aiiy
?ano?eeoacii? aeoeaiino? aaoeee.

Aeey i?iaaaeaiiy aeine?aeaeaiue aoei a?ae?a?aii 183 eoeueoo?e aa?iaieo
nii?iooai?thth/eo aaeoa??e ?c eieaeoe?? ?inoeoooo i?e?ia?ieia?? ?
a??onieia?? IAI Oe?a?ie, na?aae ieo iocaei? eoeueoo?e, a oaeiae
?cieueiaai? c a?oioo, ?ecinoa?e ?ineei, i?aai?cio ethaeeie,
n?euenueeiainiiaea?nueeeo oa eaai?aoi?ieo oaa?ei. Naeo-niaoeeo?/i?
aiaeiioeeaace (NNA) aoei aeyaeaii o 76 ooaiao. Niaoeeo?/i?noue
ciaeaeaieo oa?iaio?a aaeaeiny aecia/eoe aeey a?eueoino? eoeueoo?, aea
aeey aeayeeo iayai?noue aiaeiioeeaac aoei o?eueee iieacaii. Anueiai
aeyaeaii ?ano?eeoace oanoe ??cieo niaoeeo?/iinoae (oaae. 5).

Oaaeeoey 5

Noano?aoia niaoeeo?/i?noue aeyaeaieo aiaeiioeeaac

Iacaa ?ano?eeoaceIine?aeiai?noue ioeeaioeae?a, ui
ai?cia?ouenyI?ioioeiBce751IGGATCCBamHIBli
86IATCGATClaIBli17IGGTCTC(N)1/5PpaIBsu22ITCCGGABspMIIBsu687ICTGCAGPstIBs
u53Ae2IGGNCCSau96I

Aiae?c oi/ie ?icuaieaiiy ??cieo AeIE c a?aeiiith ia?aeiiith no?oeoo?ith
aeiaiaeeoue, ui aea/ai? oa?iaioe ? ?noeiieie ?cioeciia?aie a?aeiieo
?ano?eeoac.

A?aei?/aii caeaaei?noue /anoioe aeyaeaiiy aaeoa??e-i?iaeooeaio?a a?ae
aeii?o? ?o aeae?eaiiy. Iaea?eueoo e?euee?noue ooai?a, ye? neioacothoue
NNA, ciaeaeaii na?aae eoeueoo?, ?cieueiaaieo c a?oioo, iaeiaioo — na?aae
eoeueoo? c i?aai?cio ethaeeie ? oaa?ei. Aea inoaii? oa?aeoa?ecothoueny
iae??ciiiai?oi?oei niaeo?ii ooai?a-i?iaeooeaio?a NNA — na?aae ieo
a?ae?a?ai? eoeueoo?e, ye? i?iaeoeothoue oa?iaioe /ioe?ueio
niaoeeo?/iinoae (?en. 1).

Io?eiai? aeai? na?ae/aoue i?i oa, ui a?eueo ye 36 % aeine?aeaeaieo
eoeueoo? aaoeee oa?aeoa?ecoaaeeny ?ano?eeoaciith aeoeai?noth. Oiio
i/aaeaeii, ui i?e a?aeai?? ooai?a aaoeee ye ?aoeei??io?a AeIE i?e
aaiii-?iaeaia?ieo aeine?aeaeaiiyo, iaiao?aeii aea/aoe iayai?noue o ieo
aiaeiioeeaac ?ano?eeoe??.

Ia iniia? io?eiaieo ?acoeueoao?a aeey iiaeaeueoi? ?iaioe a?ae?a?aee
aeae?eueea ooai?a — B.subtilis 3 (iniiaa a?inii?eio); ooaie B. subtilis
2895 ? 2896, ye? oa?aeoa?ecothoueny aenieith aioaaii?noe/iith
aeoeai?noth, a oaeiae eaai?aoi?i? ooaie B. subtilis BD 170 oa BD 224,
ye? aeei?enoiaothoueny a aaiii-?iaeaia?ieo aenia?eiaioao.

O?ainoi?iaoe?th a?ae?a?aieo aaeoa??aeueieo eoeueoo? i?iaiaeeee
ieaci?aeaie na??? pBMB, ye? eiaeothoue neioac nae?aoi?iiai (?AIA 105) oa
aioo??oiueiee?oeiiiai (?AIA 104) ?ioa?oa?iio (aeine?aeaeaiiy
i?iaiaeeeeny ni?eueii n AeIOe AA “Aaeoi?”, i.Iiainea??nuee). Ieaci?aee
?AIA i?noyoue aai ?ioa?oa?iio o aeaeyae? o?i?/ii neioaciaaiiai aiaeiaa
aaia ethaenueeiai eaeeioeeoa?iiai -2 ?ioa?oa?iio. O no?oeoo?? ieaci?aee
?AIA 105 aai ?ioa?oa?iio noeeiaaiee c “aaiii” neaiaeueiiai iaioeaeo,
aiiieia?/iiai iine?aeiaiino? aaia -ai?eace, yeee caaacia/o? aoaeoeaio
nae?aoe?th c ee?oeie aaoa?ieia?/iiai a?eea. Ieaci?aea ?AIA 104 ia ia? a
no?oeoo?? oaei? iine?aeiaiino? ioeeaioeae?a ia?aae aaiii ?ioa?oa?iio,
oiio i?iaeoeiaaiee a?eie caeeoa?oueny ana?aaeei? ee?oeie.

I?e aea/aii? noaa?eueiino? ieaci?ae a ??cieo ooaiao aaoeee anoaiiaeaii,
ui iaea?eueoith noaa?euei?noth a?ae??ciy?oueny ieaci?aea ?AIA 105 a
ooai? B.subtilis 3. Iaa?oue /a?ac 10 ianaae?a ia na?aaeiaeu? aac
naeaeoeaiiai oeneo niinoa??aaeiny ?? 100 %-ia caa?aaeaiiy. Oiio
iiaeaeueo? aeine?aeaeaiiy i?iaiaeeee c ?aeiia?iaioiei ooaiii B.subtilis
3 (105).

?ano?eeoe?eiei aiae?cii, a oaeiae iaoiaeii a?a?eaeecaoe?? c o?aaiaioii
ieaci?aee, yeee i?noeoue aai ?ioa?oa?iio, iieacaii no?oeoo?io
oe?e?ni?noue ieaci?aee i?ney ianoaaiiy eoeueoo?e ia iiaeeaieo
na?aaeiaeuao aac naeaeoeaiiai oeneo ? /a?ac i?aai?ci eaai?aoi?ieo
oaa?ei.

I?e aea/aii? a?ieia?/ieo aeanoeainoae neiino?oeiaaiiai ooaio iniaeeao
oaaao i?eae?eyee aeine?aeaeaiith i?iaeoeoe?? -2 ?ioa?oa?iio. Aeey
oeueiai ia?o ca ana aiae?coaaee neeaae a?ee?a eoeueoo?aeueiiai
na?aaeiaeua i?ney ?inoo B.subtilis 3(?AIA 105), aaeaaoenue aei
aeaeo?ioi?aco a iie?ae?eeai?aeiiio aae?. A eoeueoo?aeueiiio na?aaeiaeu?
?aeiia?iaioiiai ooaio aeyaeaii aeiaeaoeiaee a?eie, iiae?aiee ca nai?th
aeaeoi?ioi?aoe/iith ?ooeea?noth aei -2 ?ioa?oa?iio. A?ieia?/iei
oanooaaiiyi, a oaeiae ?ioiioa?iaioiei aiae?cii i?aeoaa?aeaeaii
iayai?noue ?ioa?oa?iio a eoeueoo?aeueiiio na?aaeiaeu? i?ney ?inoo
B.subtilis 3(?AIA 105) ? anoaiiaeaii, ui eoeueoo?a i?iaeoeo? ?ioa?oa?ii
a e?eueeino? 104 — 105 II/ie eoeueoo?aeueiiai na?aaeiaeua. Ii?yae c
oe??th iiaith aeey ooaio aeanoea?noth a?i caa??a aenieo aioaaii?noe/io
aeoeai?noue iaoa?einueei? eoeueoo?e uiaei iaoiaaiieo oa oiiaii
iaoiaaiieo i?e?ii?aai?ci?a.

O aeine?aeao, i?iaaaeaieo in vitro oa ia eaai?aoi?ieo oaa?eiao,
aeiaaaeaii, ui aaaaeaia ieaci?aeia AeIE ia ia?aaea?oueny a?ae
B.subtilis 3(105) ?ioei i?e?ii?aai?ciai — ye i?aaenoaaieeai
ii?iaeueii? i?e?ioei?e, oae ? oiiaii iaoiaaiiei oa iaoiaaiiei
eoeueoo?ai.

?aeiia?iaioiee ooai B.subtilis 3(105) noaa iniiaith iiaiai i?ia?ioeea —
noaae?io.

I?iaaaeai? anaa?/i? aei?iaoaaiiy aacia/iino? (aeeth/ath/e a?noieia?/ia
aea/aiiy ??cieo i?aai?a ? oeaiei) i?aia?aoo noaae?io oa eoeueoo?e, yea
aoiaeeoue aei eiai neeaaeo, ia eaai?aoi?ieo oa n?euenueeiainiiaea?nueeeo
oaa?eiao aeiaaee, ui i?e ??cieo niiniaao aaaaeaiiy (a oiio /ene? ?
ia?aioa?aeueii) a aeicao, ye? cia/ii ia?aaeuothoue ?aeiiaiaeiaai? aei
canoinoaaiiy, i? eoeueoo?a, i? i?aia?ao ia aeeeeeathoue o oaa?ei
aoaeue-yeeo iaoieia?/ieo ci?i.

?icae?e 10. aoaeoeai?noue a?inii?eio ? noaae?io i?e aenia?eiaioaeueieo
aaeoa??aeueieo ? a??onieo ?ioaeoe?yo

Niaoeeo?/io aeoeai?noue i?ia?ioee?a aea/aee i?e ??cieo
aenia?eiaioaeueieo ?ioaeoe?yo ia eaai?aoi?ieo oaa?eiao.

?acoeueoaoe i?iaaaeaieo aeine?aeaeaiue na?ae/aoue i?i oa, ui iaa?oue
iaeii?aciaa i?io?eaeoe/a aaaaeaiiy a?inii?eio ? noaae?io, ni?e/eiy?
aenieee i?ioaeoeaiee aoaeo i?e eaii?eiaaeoa??ici?e ?ioaeoe??. ??aaiue
caoenoo i?e LD50 neeaaea? 90-100 % (?en.2), a i?e LD100 — 80 % (?en.
3). I?e oeueiio ia iii?/aia ??cieoey a aoaeoeaiino? i?ia?ioee?a.

Aeniea aioaaii?noe/ia aeoeai?noue a?inii?eio ? noaae?io uiaei ooai?a
eaii?eiaaeoa??e, ca?a?no?iaaia in vitro, ei?aeth? c ?o aeoeai?noth a
aeine?aeao ia oaa?eiao. Aeey a?inii?eio oaeee aca?iica’ycie
i?iaeaiiino?iaaii uiaei ?ioeo oiiaii iaoiaaiieo i?e?ii?aai?ci?a
(naeueiiiae, oeaae, eaiaeeae, noao?eieie?a, eeaan??e, i?ioa?a oa ?i.)
i?e ii??aiyeueiiio aiae?c? ?acoeueoao?a ee?i?/ieo aei?iaoaaiue ?
aioaaii?noe/ii? aeoeaiino? i?aia?aoo in vitro.

Nooo?ao ??cieoeth i?ae i?ia?ioeeaie aeyaeaii i?e aenia?eiaioaeueieo
a??onieo ?ioaeoe?yo.

Anoaiiaeaii, ui ?iaeaen aoaeoeaiino? noaae?io iaa?oue i?e iaeii?aciaiio
ia?i?aeueiiio aaaaeaii? ca aeiao aei ca?aaeaiiy a??onii a?eio neeaaea?
73, a oie /an ye aeey a?inii?eio — 27 (?en. 4).

I?e aaaaoi?aciaiio aaaaeaii? i?aia?ao?a (ca 12 aiaeei aei ?io?eoaaiiy, a
iio?i ua aea?/? c ?ioa?aaeii o 12 aiaeei) caoeniee aoaeo noaae?io aoa
oaeiae aeuei. I?e ca?eueoaii? aeice a??ono aeey ca?aaeaiiy ??cieoey a
i?ioaeoeaieo aeanoeainoyo i?aia?ao?a ca?eueooaaeany. Oae, i?e
?io?eoaaii? a??onii a?eio a aeic? 10LD50 ?iaeaen aoaeoeaiino? noaiiaea
70 aeey noaae?io ? 40 aeey a?inii?eio, a i?e 100LD50 — 50 ? iaioa ca
10, a?aeiia?aeii (?en. 5).

Aiaeia?/i? ?acoeueoaoe io?eiaii i?e aenia?eiaioaeuei?e aa?iana??oni?e
?ioaeoe??. Canoinoaaiiy i?aia?ao?a iaeii?aciai iaoiiaeei ?iaeaen
aoaeoeaiino? aeey noaae?io 65, aeey a?inii?eio — 33. I?e o?e?aciaiio
aaaaeaii? i?aia?ao?a ??cieoey a aoaeoeaiino? i?ia?ioee?a aoea oei
aeuith, /ei a?eueoa aeica a??ono aeei?enoiaoaaeany aeey ca?aaeaiiy
oaa?ei.

Aenieo aioea??onio aeoeai?noue noaae?io iieacaii ia iiaeae?
aai?oaeueiiai aa?iano o ii?nueeeo naeiie i?e i?noeaaiio oa ?aeoaeueiiio
canoinoaaii?. Aoei anoaiiaeaii, ui noaae?i ia iinooia?oueny nai?th ai
oea??oniith ae??th eiia?oe?eiiio i?aia?aoia? ?eaeinoeio.

I?e iniaeeaiiaaacia/i?e ?ioaeoe??, iaoiiaeai?e a??onii aioeaoaeii??e?oo
eiiae, o?eueee noaae?i aeyaeany aoaeoeaiei caniaii caoenoo oaa?ei.
Iieacieee caoenoo noaiiaeee 30%, ui a?aeiia?aea? iii??iiio caoenoo,
yeee niinoa??aa?oueny i?e ia?aioa?aeueiiio aaaaeaii? ?aeiia?iaioiiai -2
?ioa?oa?iio — ?aaoa?iio.

?icae?e 11. aeaye? iaoai?cie a?ieia?/ii? ae?? i?aia?ao?a c aaoeee
ia iae?ii?aai?ci

Anoaiiaeaii, ui i?e ia?i?aeueiiio aaaaeaii? i?ia?ioee?a aai /enoeo
eoeueoo?, ye? aoiaeyoue aei ?o neeaaeo, /anoeia aeeoo?caeaoieo aaeoa??e
(i?eaeecii 0,1%) a ia?o? oaeeeie i?iieea? a e?ia ? i?aaie eaai?aoi?ieo
oaa?ei, ia aeeeeeath/e i?e oeueiio i?yeeo iaoieia?/ieo i?ioean?a. O
iiaeaeueoiio aaeoa??? oaeaeei ae?i?iothoueny ye c e?ia?, oae ? c
aioo??oi?o i?aai?a.

A?aecia/ai? oaeoe aeaaaee i?aenoaao i?eioneaoe, ui o?aineieaoe?y
ia?i?aeueii aaaaeaieo i?e?ii?aai?ci?a ? iaeiei c iaoai?ci?a ae??
a?ii?aia?ao?a, yeee nooo?ai iineeth? caoeni? ?aaeoe?? iae?ii?aai?cio.

Oiio ianooiiei aoaiii ?iaioe aoei aea/aiiy aieeao i?ia?ioee?a ia aeaye?
iieacieee ?ioiii? ?acenoaioiino? i?aai?cio.

Aeyaeaii, ui ye aioo??oiuei/a?aaia, oae ? ia?i?aeueia aaaaeaiiy
a?inii?eio ? noaae?io noeioeth? ooieoe?iiaeueio aeoeai?noue iae?ioaa?a
ia?eoiiaaeueiiai aenoaeaoo. Iniaeeaee ?ioa?an aeeeeeaee aeai? i?i ci?io
ooieoe?iiaeueii? aeoeaiino? iae?ioaa?a i?e ia?i?aeueiiio canoinoaaii?
i?ia?ioee?a. Iieacaii, ui i?e iaeii?aciaiio ia?i?aeueiiio aaaaeaii?
oeeo i?ia?ioee?a niinoa??aa?oueny iinooiiaa aeoeaaoe?y iae?ioaa?a c
iaeneioiii /a?ac 4 aiaeeie (?en. 6). sse ? i?e aioo??oiuei/a?aaiiio
aaaaeaii?, ??aaiue noeioeyoe?? iae?ioaa?a caeaaeeoue a?ae e?eueeino?
ia?i?aeueii aaaaeaieo i?e?iaieo ee?oei; iaeaeu? cia/aiiy ?iaeaeno
noeioeyoe?? ca?a?no?iaaii i?e canoinoaaii? i?ia?ioee?a o aeic? 109
i?e?iaieo ee?oei.

Aeoiaey/e c io?eiaieo aeaieo i?i noeioeyoe?th a?inii?eiii ? noaae?iii
ooieoe?iiaeueii? aeoeaiino? iae?ioaa?a, aaaaeaee, ui aeioe?eueii aea/eoe
aieea oeeo i?ia?ioee?a ia ?io? iieacieee ianiaoeeo?/ii? ?acenoaioiino?
iae?ii?aai?cio, cie?aia, ia ?iaeoeoe?th aiaeiaaiiiai ?ioa?oa?iio.

I?iaaaeai? aeine?aeaeaiiy ia eaai?aoi?ieo oaa?eiao iieacaee, ui
i?ia?ioeee c aaoeee ? aoaeoeaieie ?iaeoeoi?aie ?ioa?oa?iio. ??aaiue
?iaeoeoe?? a?ae??ciy?oueny a ??cieo i?aaiao (a?ae 2.102 aei 6,4.103
II/ie):iaeaeu? cia/aiiy ?a?no?othoueny a oeion?, e?ioaoe/ieo aoceao,
naeac?ioe?. Nooo?aa ??cieoey i?ae a?inii?eiii ? noaae?iii a?aecia/aia ca
caeaoi?noth ?iaeoeoaaoe aiaeiaaiiee ?ioa?oa?ii a eeoa/ieeo. Oae, /a?ac 4
aiaeeie i?ney iaeii?aciaiai ia?i?aeueiiai aaaaeaiiy noaae?io ??aaiue
?ioa?oa?iio a eeoa/ieeo noaiiaea 1,6.103 II/ie ? caa??aaany aei 24
aiaeei. I?e aaaaeaii? a?inii?eio ?iaeoeoe?? ?ioa?oa?iio a eeoa/ieeo ia
a?aeaoaa?oueny.

Ine?eueee ooai, ui aoiaeeoue aei neeaaeo noaae?io, oa?aeoa?eco?oueny
caeaoi?noth i?iaeoeoaaoe -2 ?ioa?oa?ii ethaeeie, iaiao?aeii aoei
anoaiiaeoe, /e i?iaeoeo?oueny ?ioa?oa?ii a i?aai?ci? i?e ia?i?aeueiiio
aaaaeaii? i?ia?ioeea. Aeey oeueiai aiiiaaiaoe aioo??oi?o i?aai?a ieoae,
yeei aaiaeeee noaae?i, ia?aa??yee ia iayai?noue -2 ?ioa?oa?iio ethaeeie.
?ioa?oa?ii -2 ethaeeie aeyaeaii o an?o oanoiaaieo i?aaiao. Iaea?eueoo
e?euee?noue eiai ca?a?no?iaaii a ia/?ioe? (3,2.103 — 6,4.103 II/ie),
eaaaiyo (8,0.102 — 1,6.103 II/ie) ? eeoa/ieeo (8,0.102 — 3,2.103 II/ie).

Oaeei /eiii, i?iaaaeai? aeine?aeaeaiiy aeiaaee, ui i?ia?ioeee c aaoeee ?
aoaeoeaieie ?iaeoeoi?aie aiaeiaaiiiai ?ioa?oa?iio. Noaae?i i?e
ia?i?aeueiiio canoinoaaii? i?iaeoeo? a ??cieo i?aaiao -2 ?ioa?oa?ii
ethaeeie. Iiaeeeai, caoeniee aoaeo a?inii?eio i?e a??onieo ?ioaeoe?yo
iia’ycaiee c ?iaeoeoe??th aiaeiaaiiiai ?ioa?oa?iio, ine?eueee aeyaeaia
e?euee?noue ?ioa?oa?iio aeinoaoiy aeey i?ioea??oniiai caoenoo.

Aea/aee aieea ?ic?iaeaieo iaie i?ia?ioee?a ia niaoeeo?/io ?ioiio
a?aeiia?aeue i?e aaeoeeiaoe??. Anoaiiaeaii, ui iaa?oue iaeii?aciaa
ia?i?aeueia aaaaeaiiy a?inii?eio ? noaae?io oaa?eiai ia?aae
a?eoeeiaoe??th iaoiiaeth? a?eueo o?eaaeee ? aenieee ??aaiue niaoeeo?/ii?
?ioiii? a?aeiia?ae? aei aeeooa??eiiai oa i?aaoeaaiai eiiiiiaio?a
AEAeI-aaeoeeie.

Aiaeia?/i? ?acoeueoaoe io?eiaii i?e aeei?enoaii? ?ioeo aaeoeei. Oae,
?ioi?caoe?y aaeoeeiith c ee?i?/iiai ooaio Candida albicans 335 aoea
aoaeoeai?oith i?e iaeii/aniiio ia?i?aeueiiio aaaaeaii? i?ia?ioee?a c
aaoeee (?en. 7).

Oaeei /eiii, oai?aoe/ii iaa?oioiaai? ? i?aeoe/ii ?aae?ciaai? i?aeoiaee
aei noai?aiiy ia iniia? aaeoa??e ?iaea Bacillus a?ii?aia?ao?a c
oi?eaeueieie aeanoeainoyie. Iaea?aeai? aeai? i?i iiaeeea?noue
eiino?othaaiiy iaoiaeaie aaiaoe/ii? ?iaeaia??? ?aeiia?iaioieo ooai?a c
caieaiiaaieie aeanoeainoyie a?aee?eaathoue iia? iai?yiee ?ic?iaee
a?ii?aia?ao?a ? aea/aiiy iaoai?ci?a aca?iiae?? iae?i- ? i?e?ii?aai?ci?a.

aeniiaee

1. Aa?iai? nii?iooai?thth/? aaeoa??? ?iaeo Bacillus ? iaa?ae’?iiei
eiiiiiaioii aeciaaiii? i?e?ioei?e oaieie?iaieo. Na?aae ?ioeo
i?aaenoaaiee?a aeciaaiii? i?e?ioei?e aaoeeee oa?aeoa?ecothoueny
ia?aaaaaie, ye? aeicaieythoue aaaaeaoe ?o iaea?eueo aoaeoeaieie aeey
noai?aiiy iiaeo i?ia?ioee?a.

2. Ia iniia? aea/aiiy a?ieia?/ieo iniaeeainoae aa?iaieo
nii?iooai?thth/eo aaeoa??e ?ic?iaeaii i?eioeeiiai iiaee aenieiaoaeoeaiee
i?ia?ioee a?inii?ei (iaoaio Oe?a?ie ? 689 ? iaoaio ?inn?enueei?
Oaaea?aoe?? ? 1722502). Aei neeaaeo i?aia?aoo aeeth/aii aea? eoeueoo?e
aaoeee, ye? aeiiiaiththoue iaeia iaeio ca o?c?ieiai-a?io?i?/ieie
aeanoeainoyie oa niaoeeo?/iith aeoeai?noth.

3. ?ic?iaeai? iaoeia? i?eioeeie eiino?othaaiiy ?aeiia?iaioieo aaoeee c
caieaiiaaieie aeanoeainoyie, ia aac? yeeo noai?aii a?ii?aia?ao noaae?i,
ui ia ia? aiaeia?a o na?oia?e i?aeoeoe? (iaoaioe ?in?enueei?
Oaaea?aoe?? ? 1839459, 2035185).

4. A?inii?ei oa?aeoa?eco?oueny oi?eaeueiei niaeo?ii aioaaii?noe/ii?
aeoeaiino? uiaei iaoiaaiieo oa oiiaii iaoiaaiieo i?e?ii?aai?ci?a ? ia
aieeaa? iaaaoeaii ia i?aaenoaaiee?a ii?iaeueii? i?e?ioei?e. Ia a?aei?io
a?ae a?aeiieo i?ia?ioee?a, a?inii?ei i?eai?/o? ?icaeoie eaiaeeae,
e??nei?e, eaii?eiaaeoa??e.

5. Aea?aeaai? ee?i?/i? aei?iaoaaiiy a?inii?eio iieacaee eiai aenieo
i?io?eaeoe/io ? e?eoaaeueio aoaeoeai?noue i?e aino?eo eeoeiaeo
?ioaeoe?yo, a oaeiae aeey ei?aeoe?? i?e?ioei?e i?e aeenaaeoa??icao
??cii? ao?ieia??.

6. A?inii?ei ia?aaa?oo? a?aeii? ca?oa?aei? eiia?oe?ei? i?aia?aoe c
aaoeee ca iniiaieie iieacieeaie aacia/iino? oa niaoeeo?/ii? aeoeaiino?.
C on?o aea/aieo i?ia?ioee?a, iniiaaieo ia aaoeeeao, o?eueee a?inii?ei
oa?aeoa?eco?oueny aioaaii?noe/iith aeoeai?noth uiaei iaoiaaiieo oa
oiiaii iaoiaaiieo i?e?ii?aai?ci?a, a oiio /ene? ? iie??acenoaioieo aei
aioea?ioee?a.

7. Noaae?i ii?yae c aenieith aioei?e?iaiith aeoeai?noth aei oe?ieiai
niaeo?a iaoiaaiieo oa oiiaii iaoiaaiieo i?e?ii?aai?ci?a
oa?aeoa?eco?oueny aioea??onieie aeanoeainoyie ca ?aooiie neioaco
?ioa?oa?iio ethaeeie -2 oeio. ?iaeaen aoaeoeaiino? i?e iaeii?aciaiio
ia?i?aeueiiio aaaaeaii? noaae?io noaiiaeoue 73 i?e a?eiici?e ?ioaeoe?? ?
65 — i?e aenia?eiaioaeuei?e aa?iana??oni?e ?ioaeoe??. I?e aaaaoi?aciaeo
aaaaeaiiyo noaae?io aoaeoeai?noue caoenoo caeaaeeoue a?ae aeice
aaaaeaiiai a??ono.

8. I?iaeaiiino?iaaii aoaeoeai?noue a?inii?eio ? noaae?io i?e
aenia?eiaioaeuei?e eaii?eiaaeoa??ici?e ?ioaeoe??. ??aaiue caoenoo
iaeiaeiaee aeey iaio i?aia?ao?a ? i?e LD50 noaiiaeoue 90-100 %, a i?e
LD100 — 80 %.

9. Aia?oa anoaiiaeaii, ui i?e ia?i?aeueiiio aaaaeaii? i?ia?ioee?a aai
eoeueoo?, ye? neeaaeathoue ?o iniiao, /anoeia aeeoo?caeaoieo aaeoa??e
(a?ey 0,1%) a ia?o? oaeeeie i?iieea? a e?ia ? i?aaie oaieie?iaieo, ia
aeeeeeath/e i?e oeueiio i?yeeo iaoieia?/ieo i?ioean?a. Iieacaii, ui
o?aineieaoe?y aaeoa??e-i?ia?ioee?a ? iaeiei c oaeoi??a noeioeyoe??
?ioiii? ?acenoaioiino? iae?ii?aai?cio.

10. A?inii?ei ? noaae?i i?e ia?i?aeueiiio aaaaeaii? cia/ii i?aeaeuothoue
ianiaoeeo?/io ?ioiio ?acenoaioi?noue iae?ii?aai?cio, a oaeiae
niaoeeo?/io ?ioiio a?aeiia?aeue i?e canoinoaaii? ?o i?ae /an
aaeoeeiaoe??.

11. ?ic?iaeaii oa caoaa?aeaeaii a onoaiiaeaiiio ii?yaeeo iaiao?aeio
ii?iaoeaio aeieoiaioaoe?th aeey canoinoaaiiy a?inii?eio a iaaeeoeei?, a
noaae?io — a aaoa?eia?i?e i?aeoeoe?.

NIENIE ?IA?O, UI IIOAE?EIAAI? CA OAIITH AeENA?OAOe??

1. Nie?iia A.A., ?aciee N.?., Ni?ieoeiaa E.A. Iaoiaee/aneea
?aeiiaiaeaoeee ii auaeaeaieth e eaeaioeoeeaoeee aaeoa?ee ?iaea Bacillus
ec i?aaiecia /aeiaaea e aeeaioiuo — Eeaa. — 1983. — 50 n.

2. Ni?ieoeiaa E.A. Ia?niaeoeau i?eiaiaiey aaeoa?ee ?iaea Bacillus aeey
eiino?oe?iaaiey iiauo aeii?aia?aoia. // Aioeaeioeee e oeieioa?aiey. —
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4. Ni?ieoeiaa E.A. Eco/aiea aaciianiinoe e ?aaeoiaaiiinoe iiaiai
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60, ? 1. — N. 43-46.

5. Ni?ieoeiaa E.A. Aeeyiea i?iaeioeeia ec aaoeeee ia ooieoeeiiaeueioth
aeoeaiinoue iae?ioaaia // Aioeaeioeee e oeieioa?aiey. — 1998. — 43, ? 2.
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6. Smirnov V.V., Sorokulova I.B., Reznik S.R. Aerobic spore-forming
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7. Nie?iia A.A., Ni?ieoeiaa E.A., ?aciee N.?. Nii?iia?acothuea ay?iaiua
aaeoa?ee eae yoeieiae/aneee oaeoi? aaeoa?eaiee // AEo?iae aeaeaiu,
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8. Nie?iia A.A., ?aciee N.?., Ni?ieoeiaa E.A., Auethieoeeay A.A. I
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9. E?aia?ia A.I., Ne?uieia I.A., Niieyieiia A.A., Nie?iia A.A., ?aciee
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15. Belyavskaya V.A., Sorokulova I.B., Ilyichev A.A. Prospective for
constructing immune preparation based on recombinant bacilli // Intern.
J. Immunorehabilitation. -1994.- ?1, Suppl. — ?. 56.

16. *oaeiianeay I.A., ?uaaeei N.E., Ni?ieoeiaa E.A., Aaeyaneay A.A.,
?aciee N.?., Nie?iia A.A. Aioeae?oniay aeoeaiinoue i?iaeioeeia ec
aaoeeee // Aeiiia?ae? Iaoe?iiaeueii? aeaaeai?? iaoe Oe?a?ie. — 1995. — ?
2. — N. 124-126.

17. Nie?iia A.A., ?aciee N.?., Auethieoeeay A.A., Ni?ieoeiaa E.A.,
Eeoaei A.I. Aeeyiea eiiieaeniiai i?iaeioeea nii?ieaeoa ia iee?iaeioeaiic
eeoa/ieea oaieie?iaiuo// Iee?iaeie. aeo?i. — 1995. — 57, ? 4. — N.
42-49.

18. A?a/aaa I.I., Aaa?eeia A.O., Nieiaueaaa A.E., Nie?iia A.A.,
Ni?ieoeiaa E.A., ?aciee N.?., *oaeiianeay I.A. Yooaeoeaiinoue iiaiai
aaeoa?eeiiai i?aia?aoa aeinii?eia i?e ea/aiee ino?uo eeoa/iuo eioaeoeee
// AEo?i. iee?iaeie. — 1996. — ? 1. — N. 75-77.

19. Ni?ieoeiaa E.A., Aaeyaneay A.A., Ianu/aaa A.E., Nie?iia A.A.
?aeiiaeiaioiua i?iaeioeee: i?iaeaiu e ia?niaeoeau eniieueciaaiey a
iaaeeoeeia e aaoa?eia?ee// Aanoiee ?inneeneie Aeaaeaiee iaaeeoeeineeo
iaoe. — 1997. — ? 3. — 46-49.

20. Sorokulova I.B., Kirik D.L., Pinchuk I.V. Probiotics against
Campylobacter pathogens// J. Travel Med. — 1997. — ? 4. — ?. 167-170.

21. Ineiiaa E.A., Ni?ieoeiaa E.A., Oa?aoeeia I.A., A?eai?ueaaa A.A.
Eco/aiea aaciianiinoe aaeoa?ee ?iaea Bacillus, ninoaaeythueo iniiao
iaeioi?uo i?iaeioeeia // AEo?iae iee?iaeieiaee, yieaeaieieiaee e
eiioiiaeieiaee. — 1998. — ? 6. — N.68-70.

22. A.n. 1398868 NNN?, IEE4 A 61 E 35/74. Niinia ea/aiey
aiieii-naioe/aneeo iinea?iaeiauo caaieaaaiee / Nie?iia A.A., ?aciee
N.?., Ni?ieoeiaa E.A., Auethieoeeay A.A., Aoiineay N.A., Aieioa A.ss.,
Aoeiiaaiiaa A.I., Aieioa E.A., Eaaithoa N.I., A?ioieueneee A.A. (NNN?).
— ? 3902829/28-14; Cayaeaii 03.06.85; Iioaeee. 30.05.88, Athe. ? 20. — 2
n.

23. A.n. 1645299 NNN?, IEE5 N 12 N 9/22, 1/20. Ooaii aaeoa?ee Bacillus
cereus — i?iaeooeaio yiaeiioeeaacu Bce 751 1/ Nie?iia A.A., ?aciee N.?.,
Ni?ieoeiaa E.A., E?aia?ia A.I., Niieyieiia A.A., Iaoaeaiei I.E. (NNN?).
— ? 4495837/13; Cayaeaii 20.10.88; Iioaeee. 30.04.91, Athe. ? 16.- 2 n.

24. A.n. 1659479 NNN?, IEE5 N 12 N 9/14, 1/20. Ooaii aaeoa?ee Bacillus
licheniformis -i?iaeooeaio ?ano?eeoacu Bli 49 1/ E?aia?ia A.I.,
Niieyieiia A.A., Iaoaeaiei I.E., Nie?iia A.A., ?aciee N.?., Ni?ieoeiaa
E.A. (NNN?). — ? 4499064/13; Cayaeaii 27.10.88; Iioaeee. 30.06.91,Athe.
? 24.- 2 n.

25. Iao. 689 Oe?a?ia, IEE5 A 61 E 39/02, 35/74.I?aia?ao a?inii?ei aeey
i?io?eaeoeee oa e?eoaaiiy oeoieiai-eeoeiaeo caoai?thaaiue ethaeeie/
Cie?iia A.A., ?aciee N.?., Ni?ieoeiaa ?.A., A’thieoeueea A.I.,
Neaainieoeueea A.O., Aa?eeei A.A., Nei?eeiaa ?.A., *aieeinueeee A.ss.,
Oioai A.A.(Oe?a?ia). — ? 4641513/13; Cayae. 06.02.89; Iioae.

26. Iao. 8443 Oe?a?ia, IEE A 61 E 35/74. Niin?a e?eoaaiiy
ai?eii-naioe/ieo i?ney?iaeiaeo caoai?thaaiue / Ni??iia A.A., ?aciee
N.?., Ni?ieoeiaa ?.A., A’thieoeueea A.I., Aoiinueea N.A., Aieioa A.ss.,
Ao?iiaaiiaa E.I., Aieioa E.A., ?aaithoa N.I., A?ioieuenueeee A.A.
(Oe?a?ia). -AI 13968868; Cayae. 03.06.85; Iioae. 30.09.96, Athe. ?
3.-2n.

27. Iao. 1722502 ?inneeneay Oaaea?aoeey, IEE5 A 61 E 39/02, 35/74.
I?aia?ao aeinii?ei aeey i?ioeeaeoeee e ea/aiey aeaeoaei/ii-eeoa/iuo
caaieaaaiee /aeiaaea / Nie?iia A.A., ?aciee N.?., Ni?ieoeiaa E.A.,
Auethieoeeay A.A., Neaainieoeeay A.O., Aa?eeei Y.A., Nei?eeiaa E.A.,
*aieeineee A.ss., Oioai A.A. (Oe?aeia) — ? 4641513/13; Cayae. 06.02.89;
Iioae. 30.03.92, Athe. ? 12. — 7 n.

28. Iao. 1839459 ?inneeneay Oaaea?aoeey, IEE5 N 12 N 1/21. Ooaii
aaeoa?ee Bacillus subtilis, iaeaaeathuee aioeae?oniie e
aioeaaeoa?eaeueiie aeoeaiinoueth / Nie?iia A.A., Aaeyaneay A.A.,
Eeuee/aa A.A., Iao?aiei A.A., Oeiioaaa E.A., Ianoa?ia A.A., ?aciee N.?.,
Ni?ieoeiaa E.A. — ? 4836055/13 ; Cayae. 07.06.90; Iioaeee.23.06.94. — 4
n.

29. Iao. 2035185 ?inneeneay Oaaea?aoeey, IEE6 A 61 E 35/66.
I?ioeeaeoe/aneee aeii?aia?ao noaaeei / Nie?iia A.A., ?aciee N.?.,
Ni?ieoeiaa E.A., Naiaeao/eaa E.N., Iao?aiei A.A., Eeuee/aa A.A.,
Aaeyaneay A.A., Oeiioaaa E.A. — ? 5025233/13; Cayae. 31.01.92; Iioaeee.
20.05.95, Athe. ? 14.- 5n.

30. Iao. 2035186 ?inneeneay Oaaea?aoeey, IEE6 A 61 E 35/66.
I?ioeeaeoe/aneee aeii?aia?ao nii?ieaeo/ ?aciee N.?., Ni?ieoeiaa E.A.,
Auethieoeeay A.A., Eeoaei A.I., Nie?iia A.A. (Oe?aeia) — ? 5054722/13;
Cayae. 09.07.92; Iioaeee. 20.05.95, Athe. ? 14. — 2 n.

31. Nie?iia A.A., Ni?ieoeiaa E.A., Ineiiaa E.A., Nicaaaa E.A., Na?eenia
N.Y. Niinia ei??aeoeee iee?ioei?u aaaeiu/ Iieiaeeoaeueiia ?aoaiea i
auaea/a iaoaioa ii cayaea ? 96122712/13 io 27.11.96 a.

32. Nie?iia A.A., Ni?ieoeiaa E.A., Ineiiaa E.A. Aeii?aia?ao — noaoeeie
aeey i?ioeeaeoeee e ea/aiey eioaeoeeiiiuo aieaciae / Iieiaeeoaeueiia
?aoaiea i auaea/a iaoaioa ii cayaea ? 98101454/13 io 04.02.98 a.

33. Iiaa?ee E.A., Oa?a/ei A.O., Eociaoeia A.A., Oeeei A.A., Aei?iiei
A.I., Nie?iia A.A., Ni?ieoeiaa E.A. Niinia iieo/aiey yoaeioeea
aeinii?eia// I?ei?eoaoiay ni?aaea ii cayaea ? 96123690

34. Smirnov V.V., Kramarov V.M., Sorokulova I.B., Reznik S.R. ,
Matvienko N.I. Biosynthesis of site-specific endonucleases by
bacteria of the genus Bacillus // «The Industrial Applications of
Enzymes».- Pisa.- 1990.- P. 14.

35. Smirnov V.V., Sorokulova I.B., Belyavskaya V.A., Reznik S.R.,
Illichev A.A., Petrenko V.A. Perspectives for use of Bacillus subtilis
recombinant strains in biopreparations.// 6-th International
Symposium on Genetics of Industrial Microorganisms. GIM 90. —
Strasbourg. — 1990. — P.21.

36. Smirnov V.V., Kramarov V.M., Sorokulova I.B., Reznik S.R.,
Matvienko N.I. Bacilli as producers of site-specific endonocleases.
//6-th International Symposium on Genetics of Industrial
Microorganisms. GIM 90. — Strasbourg. — 1990.- P.37.

37. Sorokulova I.B. Translocation of exogenous microflora through
mucous membranes of warm-blooded. //IUMS Congress: Bacteriology &
Mycology. — Osaka. — 1990. — P.149.

38. Naiai?iaeneay I.A., Ni?ieoeiaa E.A., ?aeei I.I. Eco/aiea aeaenoaey
iaeioi?uo aeii?aia?aoia ia aaeoa?ee ?iaea Yersinia // Iee?iaeie. aeo?i.
— 1994. — 56, ? 4. — N. 81-82.

39. Aaeyaneay A.A., Ianu/aaa A.E., Ni?ieoeiaa E.A., Eeuee/aa A.A.
?aeiiaeiaioiua i?iaeioeee: i?iaeaiu aaciianiinoe// Iaaeaeoia?. eiio,
iinayu. iaiyoe aeaaeaieea A.A.Aaaaa. — Iineaa. — 1996. — N.143.

40. Sorokulova I.B., Smirnov V.V. New approach to the treatment for
enteric infections // 7-th Intern. Congress Inf. Dis. — Hong Kong. —
1996. — P.188.

41. Reva O.N., Sorokulova I.B., Smirnov V.V. Computerized scheme for
identification of bacteria of the genus Bacillus and assessment of their
biotechnological importance // 9-th International Conference on Bacilli.
— Lausanne. — 1997. — P. 28.

42. Rybalko S.L., Shapiro A.W., Antonjak S.N., Dyadun S.T., Sorokulova
I.B., Grytsak T.F., Selnikova O.P. Use of eubiotics and
carbohydrate-containing bacterial biopolimers for anti-candidiasis
vaccine construction // 12th World AIDS Conference — Geneva- 1998. — P.
539.

43. Pinchuk I., Bresslier P., Verneuil B., Sorokulova I.B., Megraud F.,
Urdaci M. Inhibition of Helicobacter pylori by the probiotic strain
Bacillus subtilis 3, a potentially novel antibiotic producer //Gut.
-1998. -43, N 2, Suppl. — P. A10-A11.

Ni?ieoeiaa ?.A. Oai?aoe/ia iaa?oiooaaiiy ? i?aeoeea canoinoaaiiy
aaeoa??e ?iaeo Bacillus aeey eiino?othaaiiy iiaeo i?ia?ioee?a. —
?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy aeieoi?a a?ieia?/ieo iaoe ca
niaoe?aeuei?noth 03.00.07 — i?e?ia?ieia?y. — ?inoeooo i?e?ia?ieia?? ?
a??onieia?? ?i.Ae.E.Caaieioiiai IAI Oe?a?ie, Ee?a, 1999.

Aeena?oaoe?th i?enay/aii ieoaiiyi ?ic?iaee i?eioeeiiai iiaeo i?ia?ioee?a
ia iniia? aa?iaieo nii?iooai?thth/eo aaeoa??e ?iaeo Bacillus. Oai?aoe/ii
iaa?oioiaaii ? i?aeoe/ii ?aae?ciaaii i?aeoiaee aei noai?aiiy
a?ii?aia?ao?a c oi?eaeueieie aeanoeainoyie. Aeiaaaeaii, ui iaoiaeaie
aaiaoe/ii? ?iaeaia??? ia iniia? aaoeee iiaeia eiino?othaaoe
?aeiia?iaioi? i?ia?ioeee c caaeaieie ia?aiao?aie. I?iaaaeaii aanue
eiiieaen aeiee?i?/iiai aea/aiiy a?ii?aia?ao?a ? aeiaaaeaii ?o
aacia/i?noue aeey iae?ii?aai?cio. Iieacaii aenieo aoaeoeai?noue
?ic?iaeaieo a?ii?aia?ao?a i?e aenia?eiaioaeueieo aaeoa??aeueieo ?
a??onieo ?ioaeoe?yo, a oaeiae i?e canoinoaaii? a iaaeeoeei? oa
aaoa?eia???. Aeyaeaii iia? aniaeoe iaoai?cio ae?? cai?iiiiiaaieo
a?ii?aia?ao?a. ?ic?iaeaii oaoiieia?th ae?iaieoeoaa i?aia?ao?a oa iaoiaee
?o eiio?ieth. O anoaiiaeaiiio ii?yaeeo caoaa?aeaeaii iaiao?aeio
ii?iaoeaio aeieoiaioaoe?th, ia iniia? yei? cae?enith?oueny i?iieneiaee
aeione ?ic?iaeaieo i?ia?ioee?a — a?inii?eio ? noaae?io.

Eeth/ia? neiaa: aa?iai? nii?iooai?thth/? aaeoa??? ?iaeo Bacillus,
a?ii?aia?aoe, ?aeiia?iaioi? i?ia?ioeee, aenia?eiaioaeuei? ?ioaeoe??,
iaoai?ci ae?? i?ia?ioee?a.

Ni?ieoeiaa E.A. Oai?aoe/aneia iainiiaaiea e i?aeoeea i?eiaiaiey aaeoa?ee
?iaea Bacillus aeey eiino?oe?iaaiey iiauo i?iaeioeeia. — ?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie aeieoi?a aeieiae/aneeo iaoe ii
niaoeeaeueiinoe 03.00.07 — iee?iaeieiaey. — Einoeooo iee?iaeieiaee e
ae?onieiaee ei. Ae.E.Caaieioiiai IAI Oe?aeiu, Eeaa, 1999.

Aeenna?oaoeey iinayuaia aii?inai ?ac?aaioee i?eioeeieaeueii iiauo
i?iaeioeeia ia iniiaa ay?iaiuo nii?iia?acothueo aaeoa?ee ?iaea Bacillus.
Oai?aoe/anee iainiiaaiu e i?aeoe/anee ?aaeeciaaiu iiaeoiaeu e nicaeaieth
aeii?aia?aoia n oieeaeueieie naienoaaie.

Ia iniiaa aeaoo ooaiiia aaoeeee, eioi?ua aeiiieiytho ae?oa ae?oaa ii
oeceieiai-aeioeie/aneei naienoaai e niaeo?o aioaaiienoe/aneie
aeoeaiinoe, neiino?oe?iaai i?iaeioee aeinii?ei. Aeinii?ei ioee/aaony io
ae?oaeo ca?oaaaeiuo eiiia?/aneeo aeii?aia?aoia, aace?othueony ia
aa?iaiuo nii?iia?acothueo aaeoa?eyo (aaeoenoaoee, oea?aiaeiaai,
yia?iaa?iei) i?eaeiaeueiui niaeo?ii aioaaiienoe/aneie aeoeaiinoe e
aunieie noaiaiueth aaciianiinoe. I?e eco/aiee eeeie/aneie yooaeoeaiinoe
aeinii?eia a iaaeeoeeineeo o/?aaeaeaieyo Eeaaa, Iineau, Aeiai?iiao?ianea
ia ?aciuo eiioeiaaioao aieueiuo iieacaia aaciianiinoue i?aia?aoa, aai
oi?ioay ia?aiineiinoue, aunieay ea/aaiay yooaeoeaiinoue. Ioia/aia iieiay
yeeieiaoeey naeueiiiaee e oeaaee o anao aieueiuo, iieo/aaoeo aeinii?ei,
cia/eoaeueii nieaeaeinue eiee/anoai eee iieiinoueth yeeieio?iaaeenue
oneiaii iaoiaaiiua yioa?iaaeoa?ee, aeeth/ay iaoiaaiioth eeoa/ioth
iaei/eo (o 73,9 e 64% aieueiuo niioaaonoaaiii), iaoiaaiiua noaoeeieieee
(o 75% aieueiuo) e a?eau ?iaea Candida (o 75% aieueiuo).

Iieo/aiu oeoi?anoee?othuea aeeaaiinoe/aneea nuai?ioee aeey
eiioiioeoi?anoeaioiiai aiaeeca B.subtilis 3 e B.licheniformis 31,
ninoaaeythueo iniiao aeinii?eia, i?eiaiaiea eioi?uo iaania/eaaao
iaaeaaeioth yeni?ann-aeeaaiiinoeeo iiaeeeiiinoe i?iecaiaenoaaiiuo
ooaiiia ia anao oaoiieiae/aneeo yoaiao i?iecaiaenoaa i?aia?aoa.

A ianoiyuaa a?aiy aeinii?ei ca?aaeno?e?iaai a Oe?aeia e ?inneeneie
Oaaea?aoeee, ooaa?aeaeaia any iaiaoiaeeiay ii?iaoeaiay aeieoiaioaoeey ia
aeinii?ei — Oa?iaeiiaeiay noaouey (a Oe?aeia e ?inneeneie Oaaea?aoeee),
?aaeaiaio i?iecaiaenoaa, eino?oeoeey ii i?eiaiaieth i?aia?aoa.

I?iiuoeaiiue auione aeinii?eia inouanoaeyaony IAI “Aeiai?ioa?i”
(a.Aeiai?iiao?iane) e Oeaio?ii aiaiii-oaoie/aneeo i?iaeai
aaeoa?eieiae/aneie caueou (a. Aeaoa?eiao?a) (a niioaaonoaee n
eeoeaiceiiiui aeiaiai?ii n Einoeoooii iee?iaeieiaee e ae?onieiaee IAI
Oe?aeiu).

?ac?aaioaiu iao/iua iniiau eiino?oe?iaaiey ?aeiiaeiaioiuo i?iaeioeeia n
caaeaiiuie ia?aiao?aie iaoiaeaie aaiaoe/aneie eiaeaia?ee.
O?ainoi?iaoeeae ooaiia B.subtilis, aoiaeyuaai a ninoaa aeinii?eia,
ieacieaeiie AeIE n aaiii eaeeioeeoa?iiai -2 eioa?oa?iia /aeiaaea,
iieo/ai iiaue ooaii, eioi?ue ia?yaeo n aunieie aioaaiienoe/aneie
aeoeaiinoueth a ioiioaiee iaoiaaiiuo e oneiaii iaoiaaiiuo
iee?ii?aaieciia oa?aeoa?ecoaony aioeae?oniie aeoeaiinoueth. Iieacaii,
/oi aaaaeaiiay ieacieaeiay AeIE noaaeeueii nio?aiyaony a ?aeiiaeiaioiii
ooaiia i?e iiiai/eneaiiuo ia?anaaao eoeueoo?u a ieoaoaeueiuo n?aaeao aac
naeaeoeaiiai aeaaeaiey e i?e iannaaeao /a?ac i?aaieci eaai?aoi?iuo
aeeaioiuo. A iiuoao, i?iaaaeaiiuo in vitro e ia eaai?aoi?aiuo aeeaioiuo
aeieacaii, /oi ieacieaeiay AeIE ia ia?aaeaaony ae?oaei iee?ii?aaieciai
— eae i?aaenoaaeoaeyi ii?iaeueiie iee?ioei?u, oae e iaoiaaiiui
eoeueoo?ai.

Iieo/aiiue ooaii yaeeny iniiaie i?iaeioeea noaaeeia, eioi?ue ia eiaao
aiaeiaia a ie?iaie i?aeoeea. I?iaaaeai aanue eiiieaen aeieeeie/aneiai
eco/aiey i?aia?aoa e a ianoiyuaa a?aiy caeai/eaathony eeeie/aneea
eniuoaiey yooaeoeaiinoe noaaeeia, eioi?ua i?iaiaeyony a niioaaonoaee n
?aoaieai Eiieoaoa ii aii?inai eiioiiaeieiae/aneeo i?aia?aoia IC Oe?aeiu.

Iieacaia aunieay yooaeoeaiinoue ?ac?aaioaiiuo i?aia?aoia i?e
yenia?eiaioaeueiuo aaeoa?eaeueiuo e ae?oniuo eioaeoeeyo, a oaeaea i?e
i?iaiaiee eo a iaaeeoeeia e aaoa?eia?ee.

Auyaeaiu iaeioi?ua iiaua aniaeou iaoaiecia aeaenoaey i?aaeeiaeaiiuo
aeii?aia?aoia. Iieacaii cia/aiea o?aineieaoeee aaeoa?ee, ninoaaeythueo
iniiao i?iaeioeeia, aeey noeioeyoeee eiioiiie ?acenoaioiinoe
iae?ii?aaiecia. Onoaiiaeaia oaeaea noeioeyoeey niaoeeoe/aneiai eiioiiiai
ioaaoa i?e ia?i?aeueiii i?eiaiaee i?iaeioeeia ai a?aiy aaeoeeiaoeee.

Eeth/aaua neiaa: ay?iaiua nii?iia?acothuea aaeoa?ee ?iaea Bacillus,
aeii?aia?aou, ?aeiiaeiaioiua i?iaeioeee, yenia?eiaioaeueiua eioaeoeee,
iaoaieci aeaenoaey i?iaeioeeia.

Sorokulova I.B. Theoretical basis and practice for use of the Bacillus
genus bacteria for the construction of new probiotics. — Manuscript.

Thesis for a doctor’s degree by speciality 03.00.07 — microbiology. —
Institute of Microbiology and Virology of National Academy of Sciences
of Ukraine, Kyiv, 1999.

The dissertation is devoted to elaboration of principally new probiotics
on the base of the aerob spore-forming bacteria of the Bacillus genus.
New approaches to the creation of biopreparations with unique properties
were theoretically based and practically carried out. The possibility to
construct the recombinant probiotics with planned properties by the
methods of genetical engineering has been proved. Preclinical safety
evaluation of new probiotics has been realized. The high efficacy of
probiotics has been shown in experimental bacterial and viral infections
and during their use in medicine and veterinary. New aspects of
probiotic action have been discovered. The technology of production and
the methods of the biopreparations control were elaborated. All
necessary documentation for the production of probiotics — biosporin and
subalin — was maintained.

Key words: aerob spore-forming bacteria of the Bacillus genus,
biopreparations, recombinant probiotics, experimental infections,
mechanism of probiotic’s action.

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