1

OE?A?INUeEEE AeA?AEAAIEE OI?AA?NEOAO OA?*IAEO OAOIIEIA?E

A?AA??*AE IAOAE?ss IEEIEA?AIA

OAeE 612.3:576.8

?IC?IAEA OA AI?IAAAeAEAIIss NIINIAO IAeA?AEAIIss IAENOIIIEO
A?II?AIA?AO?A CA AeIIIIIAITH OEIOAOe??

03.00.20 — a?ioaoiieia?y

AAOI?AOA?AAO

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa oaoi?/ieo iaoe

EE?A — 1999

Aeena?oaoe??th ? ?oeiien.

?iaioa aeeiiaia a Oe?a?inueeiio aea?aeaaiiio oi?aa?neoao? oa?/iaeo
oaoiieia?e I?i?noa?noaa ina?oe Oe?a?ie.

Iaoeiaee ea??aiee: eaiaeeaeao oaoi?/ieo iaoe, aeioeaio Noaai?eiaa Ieaia
Anaaieiae?aia, Oe?a?inueeee aea?aeaaiee oi?aa?neoao oa?/iaeo oaoiieia?e.

Io?oe?ei? iiiiaioe: ae.o.i., i?ioani? Oeeaaieia na?a?e iao?iae/,
canooiiee aee?aeoi?a c iaoee ? iiai? oaoi?ee ?inoeoooo oa?/iai? o?i?? ?
oaoiieia?? Aea?aeoa?/ii?iio ? IAI Oe?a?ie.

E.a.i. Ea?iaiei aaea??e ?aaiiae/, aeioeaio eaoaae?e aeieia?? ?
a?ieia?? i?aeia?iaeiiai iacaeaaeiiai oi?aa?neoaoo “Ee?ai-Iiaeeyinueea
aeaaeai?y”.

I?ia?aeia onoaiiaa: Aea?aeaaiee oi?aa?neoao “Euea?anueea iie?oaoi?ea”,
i.Euea?a.

Caoeno a?aeaoaeaoueny: “20” aeiaoiy 1999 ? i 14 aiaeei? ia can?aeaii?
niaoe?ae?c?aaii? a/aii? ?aaee Ae 26.058.3 a Oe?a?inueeiio aea?aeaaiiio
oi?aa?neoao? oa?/iaeo oaoiieia?e ca aae?anith: 252033, Ee?a-33, aoe.
Aieiaeeie?nueea,68, ei?ion A, aoae.311.

C aeena?oaoe??th iiaeia iciaeiieoeny o a?ae?ioaoe? Oe?a?inueeiai
aea?aeaaiiai oi?aa?neoaoo oa?/iaeo oaoiieia?e ca aae?anith: 252033,
Ee?a-33, aoe. Aieiaeeie?nueea,68.

Aaoi?aoa?ao ?ic?neaiee “16 “ aa?aniy 1999 ?.

A/aiee nae?aoa? niaoe?ae?ciaaii?

a/aii? ?aaee, e.o.i. A.I.?iiiaeaiiaa

CAAAEUeIA OA?AEOA?ENOEEA AeENA?OAOe?EII? ?IAIOE

Aeooaeuei?noue oaie. A?ioaoiieia?y ia nueiaiaei?oi?e /an aeciaia
i??i?eoaoiith aaeoccth ?icaeoeo ia?iaeiiai ainiiaea?noaa a Oe?a?i?, ui
a?aei?/aii o ??oaii? ?aaee c ieoaiue iaoee oa iaoeiai-oaoi?/ii? iie?oeee
i?e I?aceaeaioia? Oe?a?ie a?ae 15. 01. 98?. «I?i iniiai? i??i?eoaoe
?icaeoeo iaoee oa iaoeiai-oaoi?/ii? iie?oeee a Oe?a?i? oa nenoaio
aea?aeaaieo iaoeiaeo oa iaoeiai-oaoi?/ieo i?ia?ai».

A?ioaoiieia?/i? iaoiaee iathoue aaeeea cia/aiiy i?e ae??oaii? i?iaeai
ioi?iie aeiae?eey, cie?aia i?e ?ic?iaoe? i?aia?ao?a aeey i/euaiiy aiaee
a?ae iaooii?iaeoeo?a. Aeina?ae aeei?enoaiiy a?ii?aia?ao?a ia iniia?
i?e?ii?aai?ci?a, ?iiia?e?ciaaieo ia i?e?iaeieo ? neioaoe/ieo iin?yo,
aeey ?ioaineo?eaoe?? i/euaiiy aiaee a?ae aoaeaaiaei?a iaooe ?
aeinoaoiuei a?aeiiei ? iioe?aiei. Iaeia c iniiaieo i?ecia/aiue iin?y —
oo?eiaiiy ee?oei i?e?ii?aai?ci?a a i?eiiaa?oiaaiio oa?? aiaee, aea
a?aeaoaathoueny i?ioeane aeano?oeoe?? i?aai?/ieo caa?oaeiaiue.

Ia?niaeoeaiei iai?yieii aeieia?/ii? a?ioaoiieia?? iiaea aooe ?ic?iaea
iaoiae?a iaea?aeaiiy a?ii?aia?ao?a ia iniia? ee?oei c? ci?iaieie
iiaa?oiaaeie aeanoeainoyie, ui aeicaieythoue ?i naiino?eii
eiioeaio?oaaoeny a iaenoiii?e a?iie?aoe? ? cia/ii i?aeaeuoaaoe
oaeaee?noue aeaa?aaeaoe?? iaooii?iaeoeo?a o aiae?.

A oaoiieia?? oaeeo a?ii?aia?ao?a iiaeeeai aeei?enoaiiy oeioaoe?eiiai
o?aeoe?iioaaiiy aaeoa??aeueieo ee?oei ca ii?oieiai-a?io?i?/ieie
aeanoeainoyie ? aeae?eaiiy o?aeoe?e ee?oei c i?aeaeuaiith caeaoi?noth
aei aaeaac?? ia iiaa?oi? ?icae?eo ??aeeia-aac.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. ?iaioa aeeth/a?
aeine?aeaeaiiy, i?iaaaeai? a ?aieao aeeiiaiiy i?ia?aie Iaoe?iiaeueiiai
eii?oaoo ii iaooe? ? oaoiieia?yi 4.1.6. “?ic?iaea iaoeiaeo iniia ?
noai?aiiy iiaeo a?ioaoiieia?/ieo i?aia?ao?a oa i?iaeoeo?a”, i?iaeo
4.1.6.28.

Iaoa ?iaioe. Iaoith ?iaioe ? ?ic?iaea niiniao iaea?aeaiiy iaenoiiieo
a?ii?aia?ao?a ca aeiiiiiaith oeioaoe?? ? aeine?aeaeaiiy aoaeoeaiino? ?o
aeei?enoaiiy aeey i/euaiiy aiaee a?ae iaooii?iaeoeo?a oa iie?auaiiy
nai?oa?iiai noaio aiaeieieu.

Iinoaaeaia iaoa i?eionea? ae??oaiiy ianooiieo caaaeaiue:

— aeine?aeaeaiiy oeioaoe?eii? caeaoiino? aaeoa??e ? ?? aca?iica’ycie c
a?ae?ioiaieie aeanoeainoyie iiaa?oi? ee?oei;

— aecia/aiiy ?aaeei?a oeioaoe?eiiai o?aeoe?iioaaiiy aaeoa??aeueieo
noniaic?e aeey aeae?eaiiy ee?oei c i?aeaeuaieie a?ae?ioiaieie
aeanoeainoyie;

— aecia/aiiy oiia i?iaaaeaiiy aaoinaeaeoe?? aaeoa??e c i?aeaeuaieie
a?ae?ioiaieie aeanoeainoyie ?o iiaa?oi? ca aeiiiiiaith oeioaoe??;

— aeine?aeaeaiiy aoaeoeaiino? i?ioeano i/euaiiy aiaee a?ae
iaooii?iaeoeo?a oa ?? iaacca?aaeaiiy i?e aeei?enoaii? iaenoiiieo
a?ii?aia?ao?a;

— ?ic?iaea ?aeiiaiaeaoe?e aeey ae?iaieoeoaa oa canoinoaaiiy
a?ii?aia?aoo.

Iaoeiaa iiaecia ?iaioe. I?iaaaeaieie aeine?aeaeaiiyie anoaiiaeaii:

— ?nioaaiiy iiceoeaii? ei?aeyoe?? i?ae oeioo?i?noth oa a?ae?ioiai?noth
iiaa?oi? ee?oei aaeoa??e;

— ?nioaaiiy a?aei?iiinoae oeioaoe?eieo o?aeoe?e ee?oei aaeoa??e ca
ii?oieia?/ieie oa a?io?i?/ieie iciaeaie;

— iiaeeea?noue aaoinaeaeoe?? iaenoiiieo oi?i aaeoa??e c aeei?enoaiiyi
oeioaoe??;

— iiaeeea?noue aeei?enoaiiy iaenoiiieo a?ii?aia?ao?a aeey iaacca?aaeaiiy
aiaeieieu oa ?ioaineo?eaoe?? i/euaiiy aiaee a?ae iaooii?iaeoeo?a.

I?aeoe/ia oe?ii?noue ?iaioe.

Aacoth/enue ia ?acoeueoaoao i?iaaaeaieo aeine?aeaeaiue, cai?iiiiiaaia
oaoiieia?y iaenoiiieo a?ii?aia?ao?a c aeei?enoaiiyi oeioaoe??. Iieacaia
aoaeoeai?noue aeei?enoaiiy iaenoiiieo a?ii?aia?ao?a aeey i/euaiiy aiaee
a?ae iaooii?iaeoeo?a oa iaacca?aaeaiiy aiaee, ui aeicaiey?
?aeiiaiaeoaaoe aeei?enoaiiy iaenoiiieo a?ii?aia?ao?a aeey e?ea?aeaoe??
aaa??eieo aeeea?a iaooii?iaeoeo?a oa iie?auaiiy nai?oa?iiai noaio
aiaeieieu.

?ic?iaeaii oeioaoe?eiee iaoiae io?eiaiiy eiioeaio?aoo nii? aaeoa??e.

Cai?iiiiiaaii iaoiae iaea?aeaiiy neio?iiieo eoeueoo? aaeoa??e i?e
aeei?enoaii? oeioaoe??.

Ai?iaaoe?y ?iaioe. Iniiai? iieiaeaiiy aeena?oaoe?eii? ?iaioe
aeiiia?aeaeeny oa iaaiai?thaaeeny Anaoe?a?inuee?e iaoeiai-oaoi?/i?e
eiioa?aioe?? ”?ic?iaea oa ai?iaaaeaeaiiy i?ia?aneaieo oaoiieia?e oa
iaeaaeaiiy o oa?/iao oa ia?a?iaio i?iieneia?noue” (Ee?a, 1995) oa ia
nooaeaionueeeo eiioa?aioe?yo OAeOOO 1989-1990??.

Ioae?eaoe??. C oaie aeena?oaoe?eii? ?iaioe iioae?eiaaii 7 ?ia?o.

Iniaenoee aianie caeiaoaa/a iieyaa? o i?iaaaeaii? aenia?eiaio?a a
eaai?aoi?ieo oa aeine?aeii-i?iieneiaeo oiiaao, i?aeaioiaoe? aei
ioae?eaoe?? ?acoeueoao?a aeine?aeaeaiue. Aiae?c ?acoeueoao?a oa ?o
ocaaaeueiaiiy i?iaaaeeeenue ni?eueii c iaoeiaei ea??aieeii.
Aei?iaoaaiiy iaenoiiiiai a?ii?aia?aoo aeey i/enoee aiaee a?ae
iaooii?iaeoeo?a a ?aaeueieo oiiaao i?iaiaeeee ni?eueii c e.a.i., no.i.n.
a?aeae?eo oaoiieia?? a?ineioaco ?inoeoooo i?e?ia?ieia?? ? a??onieia?? AI
Oe?a?ie I.I.Aeoeueaa?iaei.

Ia’?i oa no?oeoo?a ?iaioe. Aeena?oaoe?eia ?iaioa neeaaea?oueny ?c
anooio, 6 ?icae?e?a, aeniiae?a, nieneo e?oa?aoo?e ( 249 iaeiaioaaiue, a
oiio /ene? 107 ca?oa?aeieo) oa aeiaeaoeo. ?iaioa aeeeaaeaia ia 126
noi??ieao ae?oeiaaiiai oaenoo, i?noeoue 12 oaaeeoeue oa 25
?enoie?a.

?iaioa aeeiioaaeanue ia aac? eaoaae?e a?ioaoiieia?? i?e?iaiiai neioaco
Oe?a?inueeiai aea?aeaaiiai oi?aa?neoaoo oa?/iaeo oaoiieia?e, a?aeae?eo
oaoiieia?? a?ineioaco ?inoeoooo i?e?ia?ieia?? ? a??onieia?? ?i.
Ae.E.Caaieioiiai Iaoe?iiaeueii? Aeaaeai?? iaoe Oe?a?ie (?IA IAI Oe?a?ie)
i?ae ea?oaaiiyi ae.a.i. ?aaiiaa A.I., Oe?a?inueeiai ?aae?ieia?/iiai
oeaio?o, ?inoeoooo o?ce/ii? oa a?ieia?/ii? o?i?? (Anoii?y).

CI?NO ?IAIOE

O anooi? iaa?oioiaaia aeooaeuei?noue, noi?ioeueiaai? iaoa ? caaea/?
aeine?aeaeaiue, iieacai? iaoeiaa iiaecia oa i?aeoe/ia oe?ii?noue ?iaioe.
Iaaaaeai? ?acoeueoaoe i?i no?oeoo?o, aeinoia??i?noue, ai?iaaoe?th ?iaioe
oa iniaenoee aianie aeena?oaioa.

O ia?oiio ?icae?e? “Oeioaoe?y a a?ioaoiieia??” iieacaii, ui oeioaoe?th
canoiniaothoue aeey caouaiiy i?e?iaieo noniaic?e, aeey eiioeaio?oaaiiy
aeoeaiiai ioeo i?e a?io?i?/iiio i/euaii? no?/ieo aiae, a oaeiae aeey
aeae?eaiiy aeayeeo i?iaeoeo?a i?e?iaiiai neioaco. Iaaaaeaii aeai?, ui
i?e eoeueoeaoaaii? ae??aeaeae?a iiaeia canoiniaoaaoe oeioaoe?th aeey
?icae?eaiiy a?iiane ca a?eiaeie oa aaiaoe/ieie aeanoeainoyie, o?i?/iei
neeaaeii a?iiane ee?oei, a oaeiae aeaeiaei neeaaeii ci?oaieo eoeueoo? .
Ia iniia? aiae?co e?oa?aoo?ieo aeaieo c?iaeaii aeniiaie i?i
iiaeeea?noue canoinoaaiiy oeioaoe?? a i?ioeanao eoeueoeaoaaiiy aaeoa??e.

O ae?oaiio ?icae?e? “Iiaa?oiaa? aeanoeaino? ee?oei” a?aei?/aii, ui
aeaeo?ie?iaoe/i? oa a?ae?ioiai? aeanoeaino? ee?oeiii? iiaa?oi?
a?ae?a?athoue iaea?eueo aaaeeeao ?ieue a i?ioeanao aca?iiae???
i?e?ii?aai?ci?a c iiaa?oiath ?icae?eo ??aeeia-aac

Ee?oeie i?e?iaii? iiioeyoe?? a?ae??ciythoueny iaeia a?ae iaeii?
aeaeo?ie?iaoe/ieie aeanoeainoyie nai?? iiaa?oi?, iathoue ??ciee ii
aaee/ei? ca?yae aai ca’ycaiee c iei aecaoa-iioaioe?ae, ui aecia/a?
iiaeeea?noue oeioaoe?eiiai o?aeoe?iioaaiiy ee?oei.

A?aei?/aii, ui a?ae?ioiai? aeanoeaino? ee?oeie a?ae?a?athoue iaaio ?ieue
a aecia/aii? nooiaiy ?o aaeaac?? ia iiaa?oi? ?icae?eo ??aeeia-aac.
Cia/aiiy iieacieea a?ae?ioiaiino? ee?oei i?e?ii?aai?ci?a caeaaeeoue a?ae
o?ceei-o?i?/ieo oaeoi??a na?aaeiaeua ?o i?iaeeaaiiy.

Ia iniia? aiae?co e?oa?aoo?ieo aeaieo c?iaeaii aeniiaie i?i
iiaeeea?noue canoinoaaiiy oeioaoe?? aeey iaea?aeaiiy ee?oei aaeoa??e,
ye? a?ae??ciythoueny iiaa?oiaaeie aeanoeainoyie, aecia/aii iaoo ?
caaaeaiiy aeine?aeaeaiue.

O o?aoueiio ?icae?e? “Iaoa??aee ? iaoiaee aeine?aeaeaiue” iaaaaeaii
oa?aeoa?enoeeo i?e?ia?ieia?/ieo ia’?eo?a oa iiaeaii ia?ae?e iaoiae?a,
yeeie ei?enooaaeeny i?e aeeiiaii? ?iaioe.

A ?iaio? aeine?aeaeoaaeenue 12 ooai?a aaeoa??e c eieaeoe?? ?IA IAI
Oe?a?ie, ?IAe? n?euenueeiainiiaea?nueei? i?e?ia?ieia??, eaoaae?e
i?e?ia?ieia?? Iineianueeiai aea?aeaaiiai oi?aa?neoaoo (?in?y).

Eoeueoeaoaaiiy i?e?iii?aai?ci?a cae?enithaaee ia neioaoe/ieo
na?aaeiaeuao ??ciiai neeaaeo. sse aeaea?aei aoaeaoeth aeei?enoiaoaaee
aetheico, naoa?ico, aaeaeoico, aoaiie, aaenaaeaeai, aeeaoeeaiae?eieue,
aeecaeueia iaeeai. Ae?iuoaaiiy aaeoa??e i?iaiaeeee a eieaao ia ea/aeoe?
(240 ea//oa), a aia?ao? aacia?a?aiiai eoeueoeaoaaiiy AIEOI-2I, a
oa?iaioa?? “A — 0,1”. I?ioean eoeueoeaoaaiiy aaee a ia??iaee/iiio
?aaeei? oa a ?aaeei? oaiinoaoa aacia?a?aii c oaeaee?noth ?icaaaeaiiy
0,06 aiae-1. O?aeoe?iioaaiiy ee?oei aaeoa??aeueieo noniaic?e i?iaiaeeee
ia eaai?aoi?i?e oeioaoe?ei?e onoaiiaoe?.

Aecia/aiiy ii?oieia?/ii? oa a?eiai? no?oeoo?e ee?oeiieo iiioeyoe?e
cae?enithaaee ca aeiiiiiaith na?oeiai? oa aeaeo?iiii? i?e?ineii?? ia
i?e?ineiiao ETHIAI-E2 oa JAI-7. Oeeoioeoi?eiao?e/iee aiae?c
?iciiae?eaiiy ee?oei ca ai?noii AeIE, a?eea ? ?ici??aie i?iaiaeeee ca
aeiiiiiaith i?ioi/iiai oeeoioeoi?eiao?a FACStar Plus. Ai?no aoaeaaiaei?a
a i?iaao aiaee oa eoeueoo?aeuei?e ??aeei? aecia/aee iaoiaeii
?io?a/a?aiii? niaeo?ioioiiao??? ia aiae?caoi?? iaooii?iaeoeo?a AI-1.
A?ae?ioiai? aeanoeaino? iiaa?oi? ee?oei aea/aee ca iaoiaeii Rosenberg.

Aaoinaeaeoe?th iaenoiiieo oi?i aaoeee cae?enithaaee ne?aeoth/ei /eiii:
aeae?eyee o?aeoe?th oaeaeeioeiooth/eo ee?oei c i?aeaeuaieie
a?ae?ioiaieie aeanoeainoyie, aeei?enoiaoth/e ?? ye iin?aiee iaoa??ae
aeey ianooiiiai eoeueoeaoaaiiy i?e?ii?aai?ci?a aei aeinyaiaiiy
noaoe?iia?ii? oace ?inoo. Ae?iuaio eoeueoo?o a?aenoithaaee, a iio?i ca
aeiiiiiaith aaeoa??aeueii? iaoe? ia?aiineee a na?aea iiaeeaia
na?aaeiaeua. Iaenoiii? oi?ie aaeoa??e iaea?aeoaaee ia i?ioyc?
5-6-e?aoiiai ia?an?ao eoeueoo?e.

Noaoenoe/io ia?iaeo aenia?eiaioaeueieo aeaieo i?iaiaeeee ca
niaoe?aeueieie i?ia?aiaie aeey ia?niiaeueiiai eiii’thoa?a.

O /aoaa?oiio ?icae?e? “ Aeine?aeaeaiiy oeioaoe?eii? caeaoiino? ee?oei
aaeoa??e ? ?? ca’ycie c a?ae?ioiaieie aeanoeainoyie ?o iiaa?oi?”
iaaaaeaii ?acoeueoaoe aeine?aeaeaiue oeioaoe?eiiai ?icae?eaiiy
aaeoa??aeueieo ee?oei ca a?eii ia??iaee/ii? eoeueoo?e, iieacaii ci?ie
iieacieea a?ae?ioiaiino? ee?oei a oiae? ia??iaee/iiai eoeueoeaoaaiiy.
Aea/aii ii?oieia?/i? oa a?io?i?/i? iniaeeaino? oeioaoe?eieo o?aeoe?e
ee?oei aaeoa??e, iaaaaeaii oa?aeoa?enoeeo a?ae?ioiaieo aeanoeainoae
oeioaoe?eieo o?aeoe?e aaeoa??e.

Iieacaii, ui a oiae? ia??iaee/iiai eoeueoeaoaaiiy eoeueoo? aaoeee
niinoa??aa?oueny i?aeaeuaiiy a?ae?ioiaiino? iiaa?oi? ee?oei (oaae. 1),
i?e oeueiio iieaciee oeioo?iio? aeey aeine?aeaeoaaieo eoeueoo?
ca?eueooaaany: aeey Ban. thuringiensis c 33% aei 65%, Ban. licheniformis
c 3% aei 27%, Ban. megaterium c 54% aei 74%.

Oaaeeoey 1 — Ci?ia a?ae?ioiaieo aeanoeainoae iiaa?oi? ee?oei

aaoeee ca /an ia??iaee/iiai eoeueoeaoaaiiy

I?e?ii?aai?ci

*an eoeueoeaoaaiiy, aiae

Iieaciee a?ae?ioiaiino? nii?, A %8121620

Ban.thur?ng?ansis

4,50,22

7,50,12

14,20,21

22,50,09

59,4 0,18Ban.licheniformis
04,90,318,30,1510,00,1342,80,22Ban.megaterium3,00,183,50,2112,60,1230,00
,1561,90,31

Eiao?oe??io ei?aeyoe?? i?ae iieacieeii oeioo?iino? oa a?ae?ioiaiino?
neeaaea? 0,97 aeey Ban. thuringiensis, 0,85 aeey Ban. licheniformis oa
0,98 aeey Ban. megaterium . Iaea?aeai? ?acoeueoaoe na?ae/aoue, ui
a?ae?ioiai? aeanoeaino? iiaa?oi? ee?oei iathoue cia/iee aieea ia ?o
oeioaoe?eio caeaoi?noue ? oea iaiao?aeii a?aoiaoaaoe i?e ?ic?iaoe?
a?ioaoiieia?/ieo i?ioean?a eoeueoeaoaaiiy i?e?ii?aai?ci?a.

A ?acoeueoao? aeine?aeaeaiue anoaiiaeaii, ui a i?ioean? ia??iaee/iiai
eoeueoeaoaaiiy o aaoeee iineeth?oueny ?o aaeaac?y ia iiaa?oi? ?icae?eo
??aeeia-aac. Aeei?enoaiiy oeioaoe?eii? ia?iaee noniaic?? ee?oei aaoeee
aeicaiey? i?iaiaeeoe ?o a?eiaa o?aeoe?iioaaiiy: i?ia caaaa/o?oueny
nii?aie, a oie /an ye a eoeueoo?aeuei?e ??aeei? caeeoathoueny
aaaaoaoeai? ee?oeie ( ?en. 1).

?en. 1- Oeioaoe?eia o?aeoe?iioaaiiy noniaic?? ee?oei aaeoa??e:

a — iiaaoaia i?ia; a — noniaic?y o oeioaoi??; 1, 2, 3 — ai?no o
o?aeoe?yo aaaaoaoeaieo ee?oei, i?inii? ? nii? a?aeiia?aeii.

Canoiniaoth/e iaoaiaoe/io iiaeaeue oeioaoe?eiiai o?aeoe?iioaaiiy
aaeoa??aeueii? noniaic??, iiaeia ca aeiiiiiaith aaaaoinooiaiaai?
oeioaoe?? aeiaanoe ai?no nii? a eiioeaio?ao? iaeaea aei 100%.

Anoaiiaeaia iiaeeea?noue oeioaoe?eiiai ?icae?eaiiy aaeoa??aeueii?
noniaic?? ca ii?oieia?/ieie iciaeaie (?ici??e ee?oei). I?e oeueiio
o?aeoe?? oaeaeeioeiooth/eo ee?oei oa?aeoa?ecothoueny iaioei ai?noii
a?eea, i?ioa a?aei?ii?noue o ai?no? AeIE aeey oeioaoe?eieo o?aeoe?e ia
anoaiiaeaia (?en.2).

Ca?aeii aeaieo i?ioi/ii? oeeoioeoi?eiao??? oeioo?i?noue ee?oei ia
iia’ycaia c iieiaeaiiyi ee?oeie a oeeee? ?aie?eaoe?? o?iiiniiii? AeIE (c
ai?noii AeIE a ee?oei?).

Ca ?acoeueoaoaie aiae?co o?aeoe?e ee?oei aaeoa??e iaoiaeii i?ioi/ii?
oeeoioeoi?eiao??? aeyaeaii, ui ee?oeie c i?aeaeuaieie a?ae?ioiaieie
aeanoeainoyie iathoue iaio? ?ici??e. Oea i?aeoaa?aeaeo? oaeo e?aui?
oeioo?iino? ee?oei aaeoa??e, ye? ciaoiaeyoueny ia ii/aoeo ee?oeiiiai
oeeeeo. A ca’yceo c oeei iiaeia i?eionoeoe, ui a aa?oiueiio oa??
oa?iaioa?a aoaeooue ia?aaaaeaoe ae??ai? ee?oeie aaeoa??e, ye?
ciaoiaeyoueny ia ii/aoeo oeeeea ae?eaiiy.

Oeioaoe?eia o?aeoe?iioaaiiy aaeoa??aeueii? noniaic?? aeei?enoiaoaaee
aeey neio?ii?caoe?? ?iciiiaeaiiy aaeoa??e. A ?acoeueoao? oeioaoe?eii?
ia?iaee noniaic?? aaoeee iaea?aeoaaee o?aeoe?? oaeaeeioeiooth/eo ee?oei,
canoinoaaiiy yeeo ye iin?aiee iaoa??ae, iaoiiaeth? neio?ii?caoe?th
?iciiiaeaiiy aaeoa??e (?iaeaen neio?ii?caoe?? neeaaeaa 0,56 — 0,64) ?
neio?ii?caoe?th ?aie?eaoe?? AeIE (?en. 3).

?en.2 — ?iciiae?eaiiy ee?oei aaoeee o oeioaoe?eieo o?aeoe?yo:

*O — ?ioaineai?noue /a?aiii? oeoi?anoeaioe??, AN — ?ioaineai?noue
aieiaiai ?icn?thaaiiy na?oea, CO — ?ioaineai?noue caeaii?
oeoi?anoeaioe??; a — ai?no AeIE, a — ?ici??e, a — ai?no a?eea ___-
oaeaeeioeiooth/? ee?oeie —— — aeo?aeia iiioeyoe?y

Aac. thuringiensis Bac.
megaterium

?en. 3 — Neio?ii?caoe?y ?iciiiaeaiiy aaeoa??e:

0 _____ 0 — ai?no AeIE a eoeueoo?? _____ — eiioeaio?aoe?y
ee?oei

Ae260 — eiioeaio?aoe?y AeIE ( a iaeeieoeyo iioe/ii? aonoeie)

Oeioaoe?eiee iaoiae neio?ii?caoe?? aeicaiey? aea/aoe i?ioeane
aeeoo?ae?yeueiino? aaeoa??e i?ioyaii ee?oeiiiai oeeeeo.

Iieacaii, ui i?e oeioaoe?eiiio o?aeoe?iioaaii? aaeoa??aeueieo noniaic?e
iaea?eueo eaaei oeioothoueny ee?oeie, ye? iathoue i?aeaeuai? a?ae?ioiai?
aeanoeaino?. A ?acoeueoao? aeine?aeaeaiue anoaiiaeaii, ui
a?ae?ioiai?noue oaeaeeioeiooth/eo aaeoa??e oei a?eueoa, /ei iaioee
a?aenioie a?iiane ia?aoiaeeoue a i?iio o?aeoe?th (oaae.2).

Oaaeeoey 2 — Iieaciee a?ae?ioiaiino? oeioaoe?eieo o?aeoe?e ee?oei
aaeoa??e

Eoeueoo?a aaeoa??eA?iiana a i?ii?e o?aeoe??, % a?aeai?oAeo?aoe iia?o?y,
e/e oa

A?ae?ioiai?noue,%oaeaeeioeiooth/eo

ee?oeiee?oei, ye? caee-oeeeny a ??aeei?Bacillus 10 0,8 41,30,71
15,41,21megaterium 201,2 22,71,18 18,70,94Bacillus
10 0,8 14,60,52 6,30,75licheniformis 20 1,2
9,61,09 7,70,84Bacillus 10 0,8 39,71,01
15,40,36 thuringiensis

20 1,2 20,00,71 17,21,15

O iyoiio ?icae?e? “Aaoinaeaeoe?y iaenoiiieo oi?i aaeoa??e” ?icaeyiooi
iiaeeea?noue aaoinaeaeoe?? i?e?ii?aai?ci?a, ye? iathoue i?aeaeuai?
a?ae?ioiai? aeanoeaino?, aeey iaea?aeaiiy iaenoiiieo a?ii?aia?ao?a.

I?iaaaeaiee oeeoioeoi?eiao?e/iee aiae?c ?iciiae?eaiiy ee?oei Ban.
megaterium 1AAe, Al. paradoxus AN-1, Ps. putida AN-2 ca ?ici??aie
iieacaa, ui a iaenoiii?e ie?aoe? ia?aaaaeathoue ae??ai? ee?oeie, a oie
/an ye ee?oeie aaeoa??e, ui ciaoiaeyoueny a oiau? aiaeiiai na?aaeiaeua,
oa?aeoa?ecoaaeenue aaeeeeie ?ici??aie. Ii??aiyiiy iieaciee?a
a?ae?ioiaiino? iiaa?oi? ee?oei iaenoiiii? oa ieaieoiiii? oi?i
aeacaieo o?ueio eoeueoo? aaeoa??e i?aeoaa?aeaeo? oie oaeo, ui a oiiaao
i?e?iaeii? oeioaoe?? e?auo oeioaoe?eio caeaoi?noue iathoue ee?oeie
a?eueo ae??ai?oeo ?ici???a c i?aeaeuaiith a?ae?ioiai?noth iiaa?oi?
(?en.4 ).

I?iaaaeaii aaoinaeaeoe?th aaeoa??aeueieo eoeueoo? ca iieacieeii
a?ae?ioiaiino? ?o iiaa?oi? (?en.5 ).

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?en. 4 — A?ae?ioiai?noue iiaa?oi? ee?oei aaeoa??e :

1 — Ban. megaterium 1BD, 2 — Ps. putida AN — 2, 3 — Al. paradoxus AN —
1

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f900c700ed00d100040000002d010300050000000902ff8080000e00000024030500e200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?en.5 — A?ae?ioiai?noue iiaa?oi? ee?oei aaeoa??aeueieo eoeueoo? i?e ?o
aaoinaeaeoe?? (A,%): 1- Al. paradoxus AN-1, 2- Ps. putida AN-2, 3 —
Mathylobakterium sp. 1K, Bac. thuringiensis, 5 — Ban. megaterium 1AAe.

A i?ioean? aaoinaeaeoe?? aeine?aeaeoaaieo eoeueoo? niinoa??aa?oueny
ca?eueoaiiy iieacieea a?ae?ioiaiino? ee?oei aaeoa??e ca 6 ia?an?a?a:
aeey Al. paradoxus AN-1 a?ae 26 aei 54; Ps. putida AN-2 — a?ae 35 aei
72; Mathylobakterium sp. 1K — 31 aei 57; Bac. thuringiensis — 30 aei 54;
Ban. megaterium 1AAe- 32 aei 64.

I?e ia??iaee/iiio (96 aiae eoeueoeaoaaiiy ) oa aacia?a?aiiio (72 aiae
eoeueoeaoaaiiy c oaeaee?noth ?icaaaeaiiy 0,06 aiae-1 ) eoeueoeaoaaii?
iaenoiiii? oi?ie Ban. megaterium 1AAe ? Ps. putida AN-2 iieaciee
a?ae?ioiaiino? ee?oei ia e?iaoeue eoeueoeaoaaiiy neeaaeaa a?aeiia?aeii
63 ? 62 aeey Ban. megaterium 1AAe oa 67 ? 70 aeey Ps. putida AN-2 ,
oiaoi niinoa??aa?oueny caa?aaeaiiy a?aenaeaeoe?iiiaaii? iciaee.

O oinoiio ?icae?e? “I?aeoe/ia canoinoaaiiy iaenoiiieo aaeoa??aeueieo
a?ii?aia?ao?a” iieacaii aoaeoeai?noue aeei?enoaiiy iaenoiiieo
i?aia?ao?a aeey i/euaiiy i?e?iaeieo aiaeieieu a?ae iaooiaeo caa?oaeiaiue
oa iie?auaiiy ?o nai?oa?iiai noaio.

Anoaiiaeaia iiaeeea?noue iaea?aeaiiy iaenoiiii? oi?ie a?ii?aia?aoo ia
iniia? aaoeee, ye? iathoue oe?ieee niaeo? aioei?e?iaii? ae??, a oiio
/ene? ? ia i?aaenoaaiee?a iaoiaaiii? i?e?ioei?e. I?e aeo?eioaai? no?/ii?
aiaee c a?ii?aia?aoii i?ioyaii 48 aiaeei niinoa??aaee cieaeaiiy
caaaeueiiai i?e?iaiiai /enea oa ca?eueiaiiy a?ae i?enooiino? a aiae?
noao?eieiee?a oa oeaae, iayai?noue yeeo a aiae? na?ae/eoue i?i ??
caa?oaeiai?noue aaeoa??yie a?oie eeoa/ii? iaei/ee. Aoaeoeai?noue
iaacca?aaeaiiy caeaaeaea a?ae aeice a?ii?aia?aoo, ui aiineany
(oaae. 3).

Oaaeeoey 3 — Aoaeoeai?noue iaacca?aaeaiiy no?/ieo aiae i/enieo

nii?oae i.?aoiaa iaenoiiiei a?ii?aia?aoii

I?e?ii?-Eiio?ieue aacEiioeaio?aoe?y i?aia?aoa, 105
ee/ieaai?ciei?aia?aoa 0,93 9,3 930aiae 48
aiae24aiae 48 aiae24 aiae 48 aiae24 aiae 48 aiaeNoao?ei-

eiee

0,9×107 1,2×106

4,8×106 i/a

3,2×104 i/a

0,44×104 i/a

Oeaaee

0,9×107 2,0×103

3,1×104 i/a

2,7o104 i/a

2,1 o104 i/aCaaaeueia

i?e?iaia

/enei,

ee/ie

— —

— 10,0o106

— 12,5o104

— 11,8o103

I?ei?oea: i/a — i?eii?aai?cie ia aeyaeai?

I?aia?ao “73”, ui i?aaenoaaey? niaith iaa?? ooai?a aa?iaieo
nii?iooai?thth/eo aaoeee, ethaycii iaaeaii aeey iaoeo aeine?aeaeaiue
aeieoi?ii iaae.iaoe N.?.?aci?eii (a?aeae?e aioea?ioee?a ?i-oo
i?e?ia?ieia?? oa a??onieia?? IAI Oe?a?ie)

Ia iniia? a?aenaeaeoe?iiiaaii? eoeueoo?e Ban. megaterium 1AAe c
i?aeaeuaieie a?ae?ioiaieie aeanoeainoyie iaea?aeaii a?ii?aia?ao, yeee
aeine?aeaeoaaany ia iiaeeea?noue aeei?enoaiiy eiai aeey i/euaiiy
aiaeieieu a?ae iaooii?iaeoeo?a.

Aoaeoeai?noue aeei?enoaiiy iaenoiiii? oi?ie io?eiaiiai a?ii?aia?aoo ia
iniia? eoeueoo?e Ban. megaterium 1AAe aeey i/euaiiy aiaee a?ae
aoaeaaiaei?a iaooe ii??aithaaee c eiio?ieai — i?ioeanii naiii/euaiiy
caa?oaeiaii? aiaee oa c i?ioeanii i/euaiiy aiaee ii??aithaaee c
eiio?ieai — i?ioeanii naiii/euaiiy caa?oaeiaii? aiaee oa c i?ioeanii
i/euaiiy aiaee i?ae aieeaii noniaic?? ee?oei Ban. megaterium 1 AAe, ui
ia iaee i?aeaeuaieo a?ae?ioiaieo aeanoeainoae (?en. 6).

?en.6 — I/euaiiy aiaee a?ae aoaeaaiaei?a iaooe :

1 — eiio?ieue (aiaea aac i?aia?aoo)

2 — aiaea c noniaic??th Ban.megaterium

3 — aiaea c iaenoiiiith oi?iith ee?oei Ban.megaterium

I?e aeei?enoaii? iaenoiiii? oi?ie a?ii?aia?aoo aoaeoeai?noue i?ioeano
i/euaiiy neeaea 89%, oiae? ye a eiio?ie? oa i?e canoinoaaii?
a?ii?aia?aoo, ui ia iaa i?aeaeuaii? a?ae?ioiaiino? ee?oei, aiia neeaea
61% oa 77% a?aeiia?aeii.

Aoaeoeai?noue i/euaiiy oa oaeaee?noue aeaa?aaeaoe?? iaooii?iaeoeo?a
caeaaeeoue a?ae aeice aianaiiai a?ii?aia?aoo a aiaeo, ui i/eua?oueny
(oaae. 4).

Oaaeeoey 4 — Aieea aeice a?ii?aia?aoo ia i?ioean i/euaiiye aiaee a?ae
aoaeaaiaei?a*

Aianaiiy a?ii?a-

ia?aoo, ia/eAi?no iaooii?i-

aeoeo?a o ac??oe?,

ia/eAoaeoeai?noue aea-

a?aaeaoe?? iaooi-

i?iaeoeo?a, %Oaeaee?noue aeaa?a-

aeaoe?? iaooii?i-aeoeo?a ia/e ca aeaiueEiio?ieue (aiaea

aac a?ii?aia?aoa)

347

56,1

150,3029063,3173,0026866,11730,0020074,720300,004893,925

— Ii/aoeiaee caaaeueiee ai?no iaooii?iaeoeo?a a aiae? — 790 ia/e.

Ca?eueoaiiy aeice a?ii?aia?aoo a oeny/o ?ac (a?ae 0,3 ia/e aei 300 ia/e)
aeicaieeei i?aeaeueoe aoaeoeai?noue i/euaiiy ca 30 aei?a c 63,3% aei
93,3%, a oaeaee?noue aeaa?aaeaoe?? iaooe a 1,5 ?ace.

Aeine?aeaeoaaany aieea oaiia?aoo?e ia nooi?iue i/euaiiy aiaee a?ae
iaooii?iaeoeo?a iaenoiiiei a?ii?aia?aoii (oaae. 5).

Oaaeeoey 5 – Aieea oaiia?aoo?e ia i?ioean aeano?oeoe?? iaooii?iaeoeo?a
iaenoiiiei a?ii?aia?aoii (ii/aoeiaee ai?no iaooii?iaeoeo?a aiae? – 80
ia/e)

Oaiia?aoo?a, 0 NNooi?iue i/euaiiy , % a caeaaeiino? a?ae /ano
(ae?a)1234518

102649688930

21508599100

C iaaaaeaieo a oaae. 5 aeaieo aeaeii , ui i?e iiieaeaii? oaiia?aoo?e
nooi?iue aeano?oeoe?? aoaeaaiaei?a iaooe ciaioo?oueny a 1,5-2 ?ace.
Iaeiae, a?ae?ioiai?noue aaoeee nooo?ai ia ci?ith?oueny: ia ii/aoie
i/euaiiy neeaaeaea 0,68 ? ia e?iaoeue i/euaiiy – 0,7 aeey iaio
aa??aio?a.

Iieacaia aoaeoeai?noue aeei?enoaiiy iaenoiiii? oi?ie a?ii?aia?aoo aeey
i/euaiiy aiaeieieua a?ae iaooiaiai caa?oaeiaiiy a caieaaei? ??ee Noea.
Caa?oaeiaiiy aiaeieieua noaeiny a ?acoeueoao? aaa??eiiai ?oeioaaiy
o?oaii?iaiaeo aaciaiai eiiaeainaoo ? aoei eieae?ciaaii ia iaaaeee?e
ae?eyioe? aiaeieieua (7 o 20 i) .Aianaiiy a?ii?aia?aoo
noi?iaiaeaeoaaeinue cieaeaiiyi eiioeaio?aoe?? aoaeaaiaei?a a 3-4 ?ace
ca 90 aei?a.

A ?acoeueoao? aeei?enoaiiy iaenoiiii? oi?ie aaoeee aeey e?ea?aeaoe??
aaa??eiiai aeeeao ianoaa?eueiiai aaiceio aaee/eia caeoeo, caaaeaiiai
iaaeieeoiueiio na?aaeiaeuo ciaioo?oueny a 40 ?ac?a.

Aeey inoaoi/ii? ioe?iee aoaeoeaiino? i?aeoe/iiai aeei?enoaiiy
iaenoiiiiai a?ii?aia?aoo, i?iaaaeaii ii??aiyiiy ?acoeueoao?a i/euaiiy
aiaee a?ae caa?oaeiaiiy iaooii?iaeoeoaie ?ioeie a?aeiieie a?ii?aia?aoaie
(oaae. 6).

Oaaeeoey 6 – Ii??aiyeuei? a?aeiiino? ii aeei?enoaiith a?ii?aia?ao?a aeey
i/euaiiy aiaee a?ae iaooe ? iaooii?iaeoeo?a

Niin?a i/euaiiy

Caa?oae-ithaa/Ii/.eii-oeaio?aoe?y aoaeaai-aei?aE?ioe.eii -oeaio?aoe?y

aoaeaaiae-i?aO?eaa-e?noue i/euaiiy,ae?aNoo-i?iue i/euaiiy,%

E?oa?aoo?aAcinetobacter

deovorumAeec. iaeeai

15 ea/i3i/euaii

10

84,5A.n.NNN?

1809822A?ii?aia?ao “Yeiiaaeei”Aeec. iaeeai

5,0ie/ei/euaii

6

97,6Iaoaio ?O

2031680Acinetobacter

valentisAeec. iaeeai

7 a/i3i/euaii

5

99,0Iaoaio ?O

2053204Rhodococcus sp. AEIIS

Iaooa

14,5ia/e

0,07ia/e

20

99,5

Iaoaio ?O

2039714

A?ii?aia?ao “Aeaai?iee”

Iaooa

2,5 ia.%i/euaii

20

100Iaoaio ?O

2023686Iaenoiiiee a?ii?aia?ao

Iaooa

800 ia/e

0,04ia/e

55

99,9Iao? aeai?

sse aeaeii (oaae. 6), iaenoiiiee a?ii?aia?ao ia niia? eoeueoo?e Bacillus
megaterium 1AAe ia? aaeeeo a?iaeaa?aaeoth/o aeoeai?noue ? iiaea aooe
canoiniaaiee i?e aenieeo ii/aoeiaeo eiioeaio?aoe?yo caa?oaeithaa/a o
aeiaaeeo aaa??eieo ?iceea?a iaooii?iaeoeo?a.

Ia iniia? i?iaaaeaieo aeine?aeaeaiue ?ic?iaeaii i?aeoe/i?
?aeiiaiaeaoe?? ii ae?iaieoeoao oa ai?iaaaeaeaiith iaenoiiiiai
a?ii?aia?aoo aeey i/euaiiy aiaeieieu a?ae iaooii?iaeoeo?a. A aeiaeaoeo
iaaaaeaii ?aeiiaiaeaoe?? ii aeaioiaeaiith iaenoiiiiai a?ii?aia?aoo, ye?
i?noyoue oaoiieia?/io noaio io?eiaiiy a?ii?aia?aoo oa iien noaae?e
iniiaieo oaoiieia?/ieo i?ioean?a (?en. 7).

AENIIAEE OA ?AEIIAIAeAOe??

1. Iieacaii, ui aaaaoaoeai? ee?oeie, i?inii?e oa nii?e aaoeee
a?ae??ciythoueny ca nai?th caeaoi?noth aei oeioaoe??. Oeioaoe?eia
ia?iaea noniaic?? ee?oei aaeoa??e Bac.cereus ? Bac. subtilis aeicaiey?
i?iaiaeeoe ?o a?eiaa o?aeoe?iioaaiiy: i?ia caaaa/o?oueny nii?aie, a
oie /an ye aaaaoaoeai? ee?oeie oa i?inii?e caeeoathoueny a ??aeei?.
Aeei?enoaiiy aeainoaae?eiiai oeioaoe?eiiai o?aeoe?iioaaiiy aeicaiey?
i?aeaeueoe /anoeo nii? aei 85% aeey Bac.cereus ? aei 94% aeey Bac.
subtilis .

2. Iieacaia iiaeeea?noue iaea?aeaiiy neio?iiieo eoeueoo? ca aeiiiiiaith
oeioaoe??. Aeei?enoaiiy ye iin?aiee iaoa??ae o?aeoe?? oaeaeeioeiooth/eo
ee?oei aneio?iiieo eoeueoo? Bac. acidocaldarius ? Bac. thuringiensis
caaacia/o? neio?ii?caoe?th ?o ?iciiiaeaiiy c ?iaeaenii neio?ii?caoe??
0,56-0,64.

3. Anoaiiaeaii, ui oaeaeeioeiooth/? ee?oeie aaeoa??e iathoue iaio?
?ici??e ? i?noyoue iaioa a?eea, i?ae ee?oeie aeo?aeii? iiioeyoe??.
Iaea?eueoa caeaoi?noue aei aaeaac?? niinoa??aa?oueny o ee?oei, ui
ae?eyoueny, aai o?eueee ii ?icae?eaieo ee?oei.

4. Anoaiiaeaii, ui oeioaoe?eia caeaoi?noue ee?oei aaeoa??e caeaaeeoue
a?ae a?ae?ioiaiino? ?o iiaa?oi? i?e eiao?oe??io? ei?aeyoe?? 0,97 aeey
Bac. thuringiensis, 0,85 aeey Bac. licheniformis, 0,98 aeey Bac.
megaterium. Oeioaoe?eia o?aeoe?iioaaiiy aaeoa??aeueii? noniaic??
aeicaiey? aeae?eeoe ee?oeie c i?aeaeuaieie a?ae?ioiaieie aeanoeainoyie
i?e 10% a?aeai?? a?iiane i?iii? o?aeoe??.

5. Iiaeeeai i?iaiaeeoe aaoinaeaeoe?th iaenoiiieo oi?i aaeoa??e c
i?aeaeuaiith a 3-4 ?ace a?ae?ioiai?noth iiaa?oi? ee?oei. Aeei?enoaiiy
iaenoiiii? oi?ie aaeoa??e, iaea?aeaieo ia iniia? aa?iaieo
nii?iooai?thth/eo aaoeee, ui iathoue oe?ieee niaeo? aioea?ioe/ii?
aeoeaiino?, aeicaiey? i?iaiaeeoe a?ieia?/ia iaacca?aaeaiiy no?/ieo
aiae.

6. Iaenoiiio oi?io aaeoa??aeueieo i?aia?ao?a aeioe?eueii
aeei?enoiaoaaoe a i?ioeanao i/enoee aiaeieieu a?ae iaooiaeo
caa?oaeiaiue. I?e aeei?enoaii? iaenoiiii? oi?ie aaeoa??e B.megaterium
1AAe nooi?iue aeano?oeoe?? aoaeaaiaei?a neeaaeaea 89% a ii??aiyii? c
eiio?ieai ( aeo?aeia eoeueoo?a B.megaterium ) -77%.

7. Canoinoaaiiy iaenoiiii? oi?ie aaeoa??e B. megaterium 1AAe aeey
e?ea?aeaoe?? aaa??eiiai aeeeao ianoaa?eueiiai aaiceio o i?e?iaeiiio
aiaeieieu? aeicaiey? ciaioeoe noio caeoeo, caaaeaiiai iaaeieeoiueiio
na?aaeiaeuo a 40 ?ac?a.

8. Ca ?acoeueoaoaie eaai?aoi?ieo oa aeine?aeii-i?iieneiaeo
aeine?aeaeaiue ?ic?iaeaii ?aeiiaiaeaoe?? ii oaoiieia?? iaenoiiiiai
a?ii?aia?aoo aeey i/euaiiy aiaeieieu a?ae iaooiaeo caa?oaeiaiue.

Ioae?eaoe?? ca iaoa??aeaie aeena?oaoe??

1. Noaaieeiaa A.A., Oeeiaa?a A.A., A?aae?/ae I.I. Neio?iiecaoeey
?aciiiaeaiey aaeoa?ee n iiiiuueth oeioaoeee // Iee?iaeieiaey.-1990. -O.
59, ? 2. — n.278-282.

2. Noaaieeiaa A.A., A?aae?/ae I.I., Oa?aiaiei O.I. Iniaaiiinoe
oeioaoeee eeaoie e nii? aaoeeee / /Iee?iaeie.ae. -1991.- O.53, ? 3.
-n.37-42.

3. Noaaieeiaa A.A., Ieeueei A.N., A?aae?/ae I.I. Oeioaoeeiiiia
o?aeoeeiie?iaaiea eeaoie R- e S- aa?eaioia aaeoa?ee // Iee?iaeie.
ae.-1991. -O. 53, ? 4. — n.52-54.

4. Noaaieeiaa A.A., A?aae?/ae I.I., Oa?aiaiei O.I., Ioaeueaa A.TH.
Aaoinaeaeoeey iaenoiiiuo oi?i aaeoa?ee // Iee?iaeie. ae. -1991. -O. 53,
? 5. — n.33-36.

5. Noaaieeiaa A.A., A?aae?/ae I.I., Eaaiia A.I. Aic?anoiay
niaoeeoe/iinoue acaeiiaeaenoaey eeaoie aaoeeee n iiaa?oiinoueth ?acaeaea
aeeaeeinoue-aac // Iee?iaeieiaey. -1992. -O. 61, ? 6. — n.1038-1042.

6. Noaaieeiaa A.A., Eaaiia A.I., A?aae?/ae I.I., Aeoeueaa?ia A.I.
Eniieueciaaiea iaenoiiiuo oi?i aaoeeee aeey i/enoee e iaacca?aaeeaaiey
aiaeiaiia // Iee?iaeie. ae. -1993. -O. 55, ? 2. — n.88-93.

7. Ivanov V.N., Svechnikova T.A., Stabnikova E.V., Gregirchak N.N.
Structure of cell cycle and age structure of Bacillus population //
Iee?iaeie.ae. -1995. -O. 57, ? 4. — n.3-11.

8. Noaai?eiaa I.A., Naethe A.?., A?aa??/ae I.I. A?ioaoiieia?y i/euaiiy
a?oio?a oa aiaeiei, caa?oaeiaieo iaooii?iaeoeoaie // Anaoe?a?i.
iaoeiai-oaoi. eiio-y “?ic?iaea oa ai?iaaaeaeaiiy i?ia?aneaieo oaoiieia?e
oa iaeaaeiaiiy o oa?/iao oa ia?a?iaio i?iieneia?noue. Ee?a,
17-20 aeiaoiy. -1995. — n.105.

A?aa??/ae I.I. ?ic?iaea oa ai?iaaaeaeaiiy niiniao iaea?aeaiiy iaenoiiieo
a?ii?aia?ao?a ca aeiiiiiaith oeioaoe??. -?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa oaoi?/ieo iaoe
ca niaoe?aeuei?noth 03.00.20 — a?ioaoiieia?y. — Oe?a?inueeee aea?aeaaiee
oi?aa?neoao oa?/iaeo oaoiieia?e, Ee?a, 1999.

Aeena?oaoe?th i?enay/aii ieoaiiyi ?ic?iaee niiniao iaea?aeaiiy ?
canoinoaaiiy a?ioaoiieia?? iaenoiiieo a?ii?aia?ao?a. Iniiaith oaeeo
a?ii?aia?ao?a ? ee?oeie c i?aeaeuaiith a?ae?ioiai?noth iiaa?oi?. Oey
aeanoea?noue aeicaiey? ee?oeiai eiioeaio?oaaoeny a iiaa?oiaaiio oa??
aiaee oa ?ioaineo?eoaaoe ?? i/euaiiy. ?ic?iaeaii iiaee oeioaoe?eiee
iaoiae naeaeoe?? ee?oei aaeoa??e c i?aeaeuaieie a?ae?ioiaieie
aeanoeainoyie. Oeioaoe?y aeicaiey? a?aeae?aoe o?aeoe?? ee?oei, ye?
a?ae??ciythoueny ca nai?ie ii?oieia?/ieie oa a?io?i?/ieie aeanoeainoyie.

Cai?iiiiiaaii aeei?enoaiiy iaenoiiiiai i?aia?aoo ia iniia? Bacillus spp.
c oe?ieei niaeo?ii aioei?e?iaii? aeoeaiino? aeey iaacca?aaeaiiy
iiaa?oaaiai oa?o aiaee. Iaenoiiio oi?io Bac megaterium 1BD aeioe?eueii
aeei?enoiaoaaoe aeey i/euaiiy aiaee a?ae iaooiaeo caa?oaeiaiue.
Canoinoaaiiy oaeiai a?ii?aia?aoo a i?e?iaeieo oiiaao iieacaei cia/ia
ca?eueoaiiy a?ii/euaiiy aiaee a?ae caa?oaeiaiiy iaooii?iaeoeoaie ?
aeicaieeei ciaioeoe noio caeoeo, caaaeaiiai iaaeieeoiueiio na?aaeiaeuo a
40 ?ac?a.

Eeth/ia? neiaa: iaenoii, aaeoa??aeuei? i?aia?aoe, oeioaoe?y
i?e?ii?aai?ci?a, a?ae?ioiai?noue, oeeoioeoi?eiao??y, neio?ii?caoe?y.

A?aae?/ae I.I. ?ac?aaioea e aiaae?aiea niiniaa iieo/aiey iaenoiiiuo
aeii?aia?aoia n iiiiuueth oeioaoeee.- ?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa oaoie/aneeo iaoe
ii niaoeeaeueiinoe 03.00.20 — aeioaoiieiaey. — Oe?aeineee
ainoaea?noaaiiue oieaa?neoao ieuaauo oaoiieiaee, Eeaa, 1999.

Aeena?oaoeey iinayuaia aii?inai ?ac?aaioee niiniaa iieo/aiey e
eniieueciaaiey aeioaoiieiaee iaenoiiiuo aeii?aia?aoia. Iniiaie oaeeo
aeii?aia?aoia yaeythony eeaoee n iiauoaiiie aeae?ioiaiinoueth eo
iiaa?oiinoe, iieo/aiiua n iiiiuueth oeioaoeee.

A ?aaioa eco/aiu ii?oieiae/aneea e aeioeie/aneea iniaaiiinoe
oeioaoeeiiiuo o?aeoeee eeaoie aaeoa?ee. Ioia/aii, /oi a oiaea
ia?eiaee/aneiai eoeueoeae?iaaiey eoeueoo? aaoeeee oaaee/eaaaony
aeae?ioiaiinoue iiaa?oiinoe eeaoie, i?e yoii iieacaoaeue oeioe?oaiinoe
aeey enneaaeiaaiuo eoeueoo? oaaee/eaaaony: aeey Ban. thuringiensis n 33
aei 65, Ban. licheniformis n 3 aei 27, Ban. megaterium n 54 aei 74.
Eiaoeoeeaio ei?aeyoeee iaaeaeo iieacaoaeai oeioe?oaiinoe e
aeae?ioiaiinoe ninoaaeyao 0,97 aeey Ban. thuringiensis, 0,85 aeey Ban.
licheniformis e 0,98 aeey Ban. megaterium , /oi naeaeaoaeuenoaoao i
cia/eoaeueiii aeeyiee aeae?ioiaiuo naienoa iiaa?oiinoe eeaoie ia eo
oeioaoeeiiioth niiniaiinoue.

Eniieueciaaiea oeioaoeeiiiie ia?aaioee noniaicee aaoeeee iicaieyao
i?iaiaeeoue eo aic?anoiia o?aeoeeiie?iaaiea: iaia iaiaauaaony nii?aie, a
oi aea a?aiy a eoeueoo?aeueiie aeeaeeinoe inoathony aaaaoaoeaiua eeaoee.
Niaeanii iaoaiaoe/aneie iiaeaee oeioaoeeiiiiai o?aeoeeiie?iaaiey n
iiiiuueth iiiainooiai/aoie oeioaoeee iiaeii aeiaanoe niaea?aeaiea nii? a
eiioeaio?aoa ii/oe aei 100%.

Iieacaii, /oi i?e oeioaoeeiiiii o?aeoeeiie?iaaiee aaeoa?eaeueiuo
noniaicee iaeaieaa eaaei oeioe?othony eeaoee, eioi?ua eiatho iiauoaiiua
aeae?ioiaiua naienoaa e iaiueoea ?acia?u. Yoi iiaeoaa?aeaeaao oio oaeo,
/oi a oneiaeyo anoanoaaiiie oeioaoeee eo/ooth oeioaoeeiiioth
niiniaiinoue eiatho eeaoee aieaa iaeeeo ?acia?ia n iiauoaiiie
aeae?ioiaiinoueth iiaa?oiinoe.

I?iaaaeaii aaoinaeaeoeeth aaeoa?eaeueiuo eoeueoo? ca iieacaoaeai
aeae?ioiaiinoe eo iiaa?oiinoe. A i?ioeanna aaoinaeaeoeee enneaaeoaiuo
eoeueoo? iaaethaeaaony oaaee/aiea iieacaoaey eeaoie aaeoa?ee ca 6
ia?anaaia: aeey A. paradoxus AN-1 io 26 aei 54; Ps. putida AN-2 — io
35 aei 72; Mathylobakterium sp. 1K — io 31 aei 57; Bac. thuringiensis —
30 aei 54; Ban. megaterium 1AAe- 32 aei 64. I?e ia?eiaee/aneii (96
/an eoeueoeae?iaaiey ) oa iaia?a?aiii (72 /an eoeueoeae?iaaiey ni
nei?inoueth ?icaaaeaiey 0,06 /an-1 ) eoeueoeae?iaaiee iaenoiiiie oi?iu
Ban. megaterium 1AAe e Ps. putida AN-2 iieacaoaeue aeae?ioiaiinoe
eeaoie ia eiiaoe eoeueoeae?iaaiey ninoaaeye niioaaonoaaiii 63 e 62 aeey
Ban. megaterium 1AAe e 67 ? 70 aeey Ps. putida AN-2 , oi anoue
iaaethaeaaony nio?aiaiea ionaeaeoeeiie?iaaiiiai i?eciaea.

Onoaiiaeaia aiciiaeiinoue iieo/aiey iaenoiiiie oi?iu aeii?aia?aoa ia
iniiaa aaoeeee, eioi?ua iaeaaeatho oe?ieei niaeo?ii aioeiee?iaiiai
aeaenoaey. I?e auaea?aeeaaiee noi/iie aiaeu n aeii?aia?aoii ia
i?ioyaeaiee 48 /ania iaaethaeaee iiieaeaiea iauaai iee?iaiiai /enea e
inaiaiaeaeaiey io i?enoonoaey a aiaea noaoieieieeia e oeaae.
Yoaeoeaiinoue iaacca?aaeeaaiey caaenaea io aeicu aianaiiiai
aeii?aia?aoa.

Ia iniiaaiee ionaeaeoeeiie?iaaiiie eoeueoo?u Ban. megaterium 1AAe n
iiauoaiiuie aeae?ioiaiuie naienoaaie iieo/ai aeii?aia?ao, eioi?ue
enneaaeiaaeny ia aiciiaeiinoue eniieueciaaiey aai aeey i/enoee aiaeiaiia
io iaooai?iaeoeoia. I?e eniieueciaaiee iaenoiiiie oi?iu aeii?aia?aoa
yoaeoeaiinoue i?ioeanna i/enoee ninoaaeyea 89%, oiaaea eae a eiio?iea e
i?e eniieueciaaiee aeii?aia?aoa aac iiauoaiiie aeae?ioiaiinoe eeaoie iia
ninoaaeyea 61% e 72% niioaaonaaiii.

Yoaeoeaiinoue i/enoee e nei?inoue aeaa?aaeaoeee iaooai?iaeoeoia caaeneo
io aeicu aianaiiiai aeii?aia?aoa a i/euaaioth aiaeo. Oaaee/aiea aeicu
aeii?aia?aoa a ouny/o ?ac (io 0,3 ia/ e aei 300 ia/e) iicaieyao
iiauneoue yoaeoeaiinoue i/enoee ca 30 aeiae n 63,3% aei 93,3%, a
nei?inoue aeaa?aaeaoeee iaooe a 1,5 ?aca (ia/aeueiia niaea?aeaiea iaooe
a aiaea 790 ia/e).

Iieacaia yoaeoeaiinoue eniieueciaaiey iaenoiiiiai aeii?aia?aoa aeey
i/enoee aiaeiaia io iaooacaa?yciaiey a iieia ?aee Noea. Aianaiea
aeii?aia?aoa nii?iaiaeaeaeinue nieaeaieai eiioeaio?aoeee oaeaaiaei?iaeia
a 3-4 ?aca ca 90 aeiae. A ?acoeueoaoa eniieueciaaiey iaenoiiiie oi?iu
aaoeeee aeey eeeaeaeaoeee aaa?eeiiai ?iceeaa aaiceia aaee/eia oua?aa,
iaianaiiiai ie?oaeathuae n?aaea oiaiueoeeanue a 40 ?ac.

Ia iniiaaiee i?iaaaeaiiuo enneaaeiaaiee ?ac?aaioaiu i?aeoe/aneea
?aeiiaiaeaoeee ii i?iecaiaenoao e aiaae?aieth iaenoiiiiai aeii?aia?aoa
aeey i/enoee aiaeiaiia io iaooai?iaeoeoia.

Eeth/aaua neiaa: iaenoii, aaeoa?eaeueiua i?aia?aou, oeioaoeey
iee?ii?aaieciia, aeae?ioiaiinoue, oeeoioeoi?eiao?ey, neio?iiecaoeey.

Gregirchak N.M. Development and inculcation of recipiency method
neuston biopreparations by means of flotation . — Manuscript.

The dissertation on winning of scientific candidate degree of technical
sciences by speniality 03.00.20 — biotechnology. — Ukrainian State
University of Food Technologies, Kyiv, 1999.

The subject of the dissertation is the development of manufacturing
and application biotechnologies of neustonic biopreparation. Neustonic
biopreperation ?s based on the cells with increased hydrophobicity. This
character allows them to concentrate within the surrface layer of water
and to intensify its purification. New flotative method for the
selection of the cells with high hydrophobicity was developed. Flotation
allows to select the cell fractions, which differ by their morphological
and biochemical properties.

One neustonic biopreparation from Bacillus spp. with wide spectrum of
antimicrobial activity was proposed to use for the decontamination of
surface layer of water. Another biopreparation , which is based on
neustonic variant of Bac. megaterium 1BD, was proposed for the
bioremediation of the oil polluted water. Application of this
biopreparation in the field showed that it significantly increased the
bioremediation of water polluted by oils and decreased the sum the
damage to environmental in 40 times.

Key words: bacterial preparation, flotation of microorganisms, neuston,
hydrophobicity, flow cytofluorometry, synchronization.

17

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