Iaoe?iiaeueia Aeaaeai?y iaoe Oe?a?ie

?inoeooo iieaeoey?ii? a?ieia?? ? aaiaoeee

E?enai Einoyioei Ay/aneaaiae/

OAeE 577.29

577.218

Ia?aaoaeiae no?oeoo?e ieaci?aee pATV 8 o o?ainaaiieo ieoae

03.00.03 — iieaeoey?ia a?ieia?y

Aaoi?aoa?ao

aeenna?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo
iaoe

Ee?a — 1999

Aeenna?oaoe??th ? ?oeiien

?iaioa aeeiiaia a ?inoeooo? iieaeoey?ii? a?ieia?? ? aaiaoeee IAI
Oe?a?ie, i.Ee?a

Iaoeiaee ea??aiee: aeieoi? a?ieia?/ieo iaoe

Nieiiei Ieaenaiae? Iao?iae/

?inoeooo iieaeoey?ii? a?ieia??

? aaiaoeee IAI Oe?a?ie, i.Ee?a

caa?aeoaa/ a?aeae?eii a?io?i?/ii? aaiaoeee

Io?oe?ei? iiiiaioe: aeieoi? a?ieia?/ieo iaoe

Oaaae Aiaoie?e Aeaaeaeiae/

?inoeooo iieaeoey?ii? a?ieia??

? aaiaoeee IAI Oe?a?ie, i.Ee?a

caa?aeoaa/ a?aeae?eii iieaeoey?ii? a??onieia??

aeieoi? a?ieia?/ieo iaoe

Aaee?i Aiaoie?e Iaaeiae/

?inoeooo a?ii?aai?/ii? o?i?? oa iaooio?i??

IAI Oe?a?ie, i.Ee?a

caa?aeoaa/ a?aeae?eii a?i?iaeaia???

I?ia?aeia onoaiiaa: ?inoeooo ee?oeiii? a?ieia?? oa aaiaoe/ii?

?iaeaia??? IAI Oe?a?ie, i.Ee?a

Caoeno a?aeaoaeaoueny 18 o?aaiy 1999 ?. i 10-00 aiaeei? ia can?aeaii?
niaoe?ae?ciaaii? a/aii? ?aaee Ae.26.237.01 ?inoeoooo iieaeoey?ii?
a?ieia?? ? aaiaoeee IAI Oe?a?ie ca aae?anith:

252143, a.Ee?a-143, aoe. Aeaaeai?ea Caaieioiiai, 150.

C aeena?oaoe??th iiaeia iciaeiieoeny a a?ae?ioaoe? ?inoeoooo
iieaeoey?ii? a?ieia?? ? aaiaoeee IAI Oe?a?ie.

Aaoi?aoa?ao ?ic?neaiee 14 ea?oiy 1999 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee

eaiaeeaeao a?ieia?/ieo iaoe E.E.Eoeao

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue i?iaeaie. O?ainaaiic ia?ac? noaa iaeiei c aaaeeea?oeo
i?aeoiae?a aeey aea/aiiy iaoai?ci?a ooieoe?iioaaiiy aaiiio a oe?eiio,
aca?iiae?? eiai /anoei, ?ie? ie?aieo aai?a oa ?aaoeyoe?? ?o aeni?an??.
Oeae iaoiae oe?iei aeei?enoiao?oueny aeey aeyaeaiiy ooieoe?e ie?aieo
aai?a oeyoii ?o ni?yiiaaiiai ?oeioaaiiy aai aeiaaeeiaiai ?ina?oe?eiiai
iooaaaiaco c ianooiiei aeine?aeaeaiiyi io?eiaieo oaiioeiiaeo aoaeo?a,
aea/aiiy aeoeaaoe?? i?iiioi??a ia ??cieo aoaiao iioiaaiaco oa ?i..
Iaaecae/aeii oe?iea aeei?enoaiiy oeae iaoiae oaeiae ciaoiaeeoue i?e
?ica`ycaii? i?eeeaaeieo caaaeaiue a?ioaoiieia?? oa a?iiaaeeoeeie. Ooo
ne?ae iacaaoe ni?yiiaaio ci?io aaiioeio n?euenueeiainiiaea?nueeeo oaa?ei
aeey io?eiaiiy ii??ae, ui iathoue iia? ainiiaea?/i-oe?ii? iciaee, oa
ei?aeoe?th aaiaoe/ieo caoai?thaaiue ethaeeie oeyoii aaaaeaiiy cciai? a
?? aaiii iiaiioe?iieo eii?e aeaoaeoieo aai?a. Iaeiae, aeanoaa?e?caoe?y
aaiiio ?aoeei??ioa, aeeeeeaia aianaiiyi /oaei??aeii? aaiaoe/ii?
?ioi?iaoe??, a oaeiae ia??aeei aaaeeiia?aaeaa/oaaia iiaaae?iea
o?ainaai?a, iia’ycaia c ?o /anoith iiaeeo?eaoe??th oa iaeiio?ieueiaaiith
aeni?an??th, ?noioii oo?oaeith? aeei?enoaiiy o?ainaaiico ye a
aeine?aeieoeueeeo, oae ? a i?eeeaaeieo oe?eyo. A ca’yceo c oeei
aaaeeeaiai cia/aiiy iaaoaa? aea/aiiy aca?iiae?? i?ae o?ainaaiaie oa
aaiiiaie oacy?a, a oaeiae noai?aiiy iiaeaeueieo nenoai aeey iiooeo
caeiiii??iinoae oe??? aca?iiae??.

A?aeii? o?e iniiai? aa??aioe iiaaae?iee o?ainaai?a — iiaia ae?i?iaoe?y,
?ioaa?aoe?y a aaiii ?aoe?i?aioo oa ?nioaaiiy a aeno?ao?iiiniiiiio noai?.
sse ?ioaa?iaai?, oae ? aeno?ao?iiiniii? oi?ie o?ainaai?a /anoi
caciathoue ?noioieo iiaeeo?eaoe?e, iaoai?cie ? caeiiii??iino? yeeo
ia?ac? i?aeoe/ii ia aeine?aeaeaii. E??i oiai, ?ioaa?iaai? o?ainaaie (ia
a?aei?io a?ae a?eueoino? aeno?ao?iiiniiieo) iiaeooue aeeeeeaoe iaaaaeai?
?ina?oe?ei? iooaoe??, ui iaaea? aaaeeeaiai cia/aiiy aea/aiith
nii??aeiaiino? o?ainaai?a aei eiie?aoieo aaiiiieo ioeeaioeaeieo
iine?aeiaiinoae. C oi/ee ci?o ia?aaeaa/oaaiino? oa ea?o?iino? a?eueo
c?o/ieie aeaeathoueny o?ainaaie, caeaoi? ?nioaaoe iicao?iiiniiii.
Iaeiae, aanue eiiieaen oaeoi??a, ui aecia/athoue oi?io ?nioaaiiy
o?ainaaio oa aieeaathoue ia eiai noae?noue, iiee ui caeeoa?oueny
iaaea/aiei.

Ia i?ioyc? aaaaoueio ?ie?a o a?aeae?e? a?io?i?/ii? aaiaoeee aaaeooueny
?iaioe c aea/aiiy aca?iiae?? aaoa?ieia?/ieo aaiii?a. Nooo?ao /anoeio
oeeo ?ia?o neeaaea? aeine?aeaeaiiy aca?iiae?? aaiiio ieo? oa
?aeiia?iaioii? ieaci?aee pATV8, ui i?noeoue ioeeaioeaei? iine?aeiaiino?
aaeoa??aeueiiai aaeoi?o pBR322 oa i?ia??onii? AeIE a??ono na?eiie ?aona
(RSV). Oeae a??on ? ?aie?eaoeaii iiaiioe?iiei aenieiiieiaaiiei
?ao?ia??onii ioao?a. Ieoa ia ? ia?i?neaiei oacy?i RSV, aea eiai aeiaa?
e?ioeaa? iiaoi?e (LTR), ui i?noyoue neeueiee i?iiioi?,
aeei?enoiaothoueny i?e noai?aii? aeni?anoth/eo aaeoi??a nnaaoe?a. sse
LTR, oae ? ?io? a??oni? aaie iathoue aenieo ?aeiia?iaoe?eio aeoeai?noue.
?aeiia?iaoe?eia aeoeai?noue aaeoa??aeueieo ioeeaioeaeieo
iine?aeiaiinoae, ui aoiaeyoue aei neeaaeo a?eueoino? aoea??ioe/ieo
/iaieeiaeo aaeoi??a, oaeiae oe?eaaa ye oaeoi? aeanoaa?e?caoe?? aaiiio
?aoeei?aioo oa noaa?eueiino? o?ainaaio. O ca’yceo c oeei aaaeeeai
c’ynoaaoe oa?aeoa? aca?iiae?? iaaaaeaii? ?aeiia?iaioii? ieaci?aee oa
aaiiio ieo?; aeyaeoe aca?iiee aieea aaoa?ieia?/ieo aaiii?a; aeine?aeeoe
oaiioeiia? i?iyae iane?aee?a o?ainaaiico. Ca’ycie ?iaioe c iaoeiaeie
i?ia?aiaie, ieaiaie, oaiaie. Aeine?aeaeaiiy i?iaiaeeeeny a ?aieao
oaiaoe/ieo i?ia?ai ?IA?A IAIO: ? 2.34.1.3 “Aea/aiiy ?aaoeyoe?? aeni?an??
aaiiio ia ?aii?o noaae?yo aia??iaaiaco ieo?” (1991-1995) oa ? 2.28.7.2
“Aeine?aeaeaiiy yaea?ii-oeeoiieaciaoe/ieo aca?iiae?e a
aei?iieaioaoe?eiiio ?icaeoeo ieo?” (1996-2000).

Iaoa oa caaaeaiiy aeine?aeaeaiiy. Iaoith ?iaioe aoei aeine?aeaeaiiy
no?oeoo?ii-ooieoe?iiaeueii? aca?iiae?? o?ainaaio oa aaiiio oacy?ia, a
oaeiae aiae?c a?ieia?/ieo aoaeo?a o?ainaaiico o aeuaiaaaaeai?e
iiaeaeuei?e nenoai?. Aeey aeinyaiaiiy iinoaaeaii? iaoe, iio??aii aoei
ae??oeoe oae? caaaeaiiy:

1. Aecia/eoe oi?io ?nioaaiiy o?ainaaio.

2. Aeine?aeeoe no?oeoo?o o?ainaaio oa ?? oniaaeeoaaiiy o ieoae ??cieo
noae?i?e.

3. Aea/eoe aieea o?ainaaio ia aaiii ieo? ia oaiioeiiaiio ??ai?.

Iaoeiaa iiaecia io?eiaieo ?acoeueoao?a. Aia?oa anoaiiaeaii, ui aaaaeaiiy
?aeiia?iaioii? iieaeoee AeIE, yea i?noeea eeiiiaaio i?ia??onio AeIE
a??ono na?eiie ?aona eo?ae o ceaioe iaia?i?neaiiai oacy?ia — ieo?,
i?ecaiaeeoue aei ooai?aiiy aeaio oi?i o?ainaaio — ?ioaa?iaaii? o aaiii
oa aeno?ao?iiiniiii?. Iieacaii, ui aaaaeaia AeIE aeeeeea? nooo?ao
aeanoaa?e?caoe?th aaiiio ieo?. Aeyaeaii, ui a ?acoeueoao? aca?iiae?? c
aaiiiii ceaioe, aaaaeaia AeIE cacia? ?noioieo ia?aaoaeia o aeaeyae?
aeaeaooaaiiy cia/ii? /anoeie i?ia??onieo ioeeaioeaeieo iine?aeiaiinoae.
sse ?ioaa?iaaia, oae ? aeno?ao?iiiniiia oi?ie o?ainaaio, ci?ieaoenue
iaeii?aciai ia noaae?? ceaioe, aeae? ia?aaeathoueny ianooiiei iieie?iiyi
o?ainaaiieo ieoae aac ci?i. Aia?oa io?eiaii iieaeoeo AeIE, caeaoio aei
aeno?ao?iiiniiii? ?aie?eaoe?? oa naa?aaaoe?? o ee?oeiao o?ainaaiieo
ieoae ia i?ioyc? aaaaoueio iieie?iue. Noaa?eueio ?aie?eaoe?th
aeno?ao?iiiniiii? oi?ie o?ainaaio caaacia/othoue iiaiooai?ai? a
?acoeueoao? ia?aaoaeia eiia?iaoe?? aaeoa??aeueiiai oa a??oniiai aaiii?a.
Aia?oa iieacaii, ui aeno?ao?iiiniiiee o?ainaai ieo? oaeiae caeaoiee aei
iicao?iiiniiii? ?aie?eaoe?? o ee?oeiao eiiao aac no?oeoo?ieo ci?i.

I?aeoe/ia oe?ii?noue io?eiaieo ?acoeueoao?a. ?ic?iaeaia iiaeaeueia
nenoaia iaaea? iiaeeeaino? iioei?coaaoe aenia?eiaioe c? noai?aiiy
o?ainaaiieo oaa?ei oeyoii i?iaiicoaaiiy iiaaae?iee aeciaaiii? AeIE o
aaoa?ieia?/ieo ee?oeiao oa ?aaeoe?? aaiiio ia ?? aaaaeaiiy. Aiae?c
io?eiaieo ?acoeueoao?a aeaco? ia oa, ui i?e aaaaeaii? aeciaaiii? AeIE o
?aii? ceaioe, /ano?oa ooai?ththoueny ?ioaa?iaai?, a o i?ci? —
aeno?ao?iiiniii? oi?ie o?ainaai?a. Io?eiaiee aeno?ao?iiiniiiee o?ainaai
iiaea aooe aeei?enoaiee aeey noai?aiiy oe?ieiai niaeo?o aaeoi??a aeey
aaiioa?ai??, i?iaeoeoaaiiy a?ieia?/ii aeoeaieo i?ioa?i?a a aoea??ioe/ieo
ee?oeiao oa aeey aeine?aeieoeueeeo iio?aa.

Iniaenoee aianie caeiaoaa/a. Iniiai? ?acoeueoaoe, aeeeaaeai? o
aeena?oaoe??, io?eiaii aaoi?ii naiino?eii.

Ai?iaaoe?y ?iaioe. Iaoa??aee aeena?oaoe?? aeiiia?aeaeeny ia a?aee?eoiio
nai?ia?? a?oie c oiiae?iaaiaco, inoaiaaiaco oa ?aiiueiai ?icaeoeo
e?ioe?aie nnaaoe?a (Naaaae, Oai?ueia, eenoiiaae, 1996), iaoeia?e
eiioa?aioe?? ?inoeoooo a?io?i?? Oai?nueei? Aeaaeai?? Iaoe (Naaaae,
Oai?ueia, o?aaaiue 1997), ?iai/?e ia?aae? a?aeae?eo a?io?i?/ii? aaiaoeee
?IA?A (ethoee, 1998), i?aea?aeae?euenueeiio nai?ia?? ?IA?A (ethoee,
1999).

Ioae?eaoe??. Ci?no ?iaioe aeeeaaeaii o 3 ae?oeiaaieo ?iaioao.

No?oeoo?a oa ia’?i ?iaioe. ?iaioa neeaaea?oueny c anooio, iaeyaeo
e?oa?aoo?e, iieno iaoa??ae?a ? iaoiae?a, ?acoeueoao?a oa iaaiai?aiiy,
caeeth/aiiy oa aeniiae?a. Aeena?oaoe?th aeeeaaeaii ia noi??ieao
oaenoo. ?ethno?aoeaiee iaoa??ae i?aaenoaaeaii 1 oaaeeoeath oa 10
?enoieaie. Nienie aeei?enoaieo aeaea?ae i?noeoue 309 ?ia?o.

IAOA??AEE ? IAOIAeE

I?e?i?i’?eoaaiiy ieaci?aeii? AeIE o ceaioe ieoae. Aeiii?aie yeoeaee?oei
aeey i?iaaaeaiiy ?i’?eoe?e aoee ieo? e?i?? ICR, ai?iiiaeueii
noeioeueiaai? aeey iaea?aeaiiy neio?ii?ciaaieo yeoeaee?oei.
I?e?i?i’?eoe?? i?iaiaeeeeny o /ieia?/ee i?iioeeaon ceaio ia noaae??
aeaio i?iioeeaon?a. Aaiaeeee 200-400 eii?e ieaci?aee ia yeoeaee?oeio,
i?ney /iai ceaioe ia?anaaeaeoaaee a aiioeo yeoeaaiaeo inaaaeiaaa?oieo
naiie o??? ae naii? e?i??. Inaaaeiaaa?oieo naiie io?eioaaee oeyoii
nia?thaaiiy c aacaeoiiiaaieie naioeyie.

Aiae?c o?ainaaiieo oaa?ei. Aeio- ? aeio-aiae?c o?ainaaiieo ieoae
i?iaiaeeee, ye iienaii o [Sambrook J. et al., 1989]. Ioeeaioeaeio
iine?aeiai?noue aecia/aee ca iaoiaeii ae?aeacien?oa?i?iaoe?? (Naiaa?a)
[Sanger F. et al., 1974]. N?eaaioaaee iaeii- oa aeaieaioethaiao AeIE c
aeei?enoaiiyi 35S-aeAOO oa 32?-aeOeOO c ianooiiei ?icae?eaiiyi
o?aaiaio?a o 6% iie?ae??eai?aeiiio aeaiaoo?oth/iio aae?.
Iaeiieaioethaiao AeIE io?eioaaee c aeei?enoaiiyi aaeoa??ioaaa-iii?/ieea
VSC M 13 ca iaoiaeii, iienaiei o [Sambrook J. et al., 1989]. Aeey
n?eaaioaaiiy aeiaaeo o?aaiaio?a aeei?enoiaoaaeiny ?o nooi?i/anoa
aei?i/aiiy ca aeiiiiiaith ioeeaac Exo III oa S1 [Sambrook J. et al.,
1989].

O?ainoaeoe?y ee?oei eiiao. O?ainoaeoe?th iiiioa?iai? eoeueoo?e ee?oei
Spodoptera frugiperda e?i?? SF 21, yea ae?iuoaaeany o ieanoeeiaiio
iinoae? o??ie “Costar”, i?aia?aoaie ieaci?aeieo AeIE (5 iea AeIE ia
3o106 ee?oei) i?iaiaeeee c aeei?enoaiiyi e?iioaeoeio o??ie “Boehringer
Mannheim” a oiiaao, ?aeiiaiaeiaaieo ae?iaieeii.

?ACOEUeOAOE OA IAAIAI?AIIss

No?oeoo?a oa oa?aeoa? iiaeeo?eaoe?e ?ioaa?iaaii? oi?ie o?ainaaio.

Ooieoe?thaaiiy aaiii?a aoea??io?a (iniaeeai, ia ?aii?o aoaiao
iioiaaiaco) o?nii iia’ycaia ?c aca?iiae??th ie?aieo ?o /anoei. Oey
aca?iiae?y aeeth/a? noaae??- oa oeaieiiniaoeeo?/i? aeoeaaoe?th /e
?ai?an?th iaaieo aai?a, ca?eueoaiiy ?oiuei? aeice oeyoii aiie?o?eaoe??
oa niaaeeia? ia?aaoaeiae aaiaoe/iiai iaoa??aeo. Ii?ooaiiy oeeo i?ioean?a
o ?acoeueoao? iaoieia?/ieo ci?i o ee?oei?, no?ani?ieo aieea?a aai
aaaaeaiiy aeciaaiii? AeIE, ui caeaoia aieeaaoe ia aca?iiae?th aeaiaio?a
aaiiio, caeaoia i?ecaanoe aei ?noioieo ii?ooaiue aeeoo?ae?yeueiino?
ee?oeie oa, iaa?oue, aei caaeaae? onueiai i?aai?cio. Iiaeaethaaiiy
caaaeaieo neooaoe?e oa aea/aiiy aoaeo?a, ui aeieeathoue i?e oeueiio,
iaaea? aaaeeeao ?ioi?iaoe?th i?i ooieoe?thaaiiy aaiiio. Iaea?eueo
ia?niaeoeaiei iaoiaeii aeaea?oueny aaaaeaiiy aeciaaiieo AeIE o
i?iioeeaon caie?aeiaieo yeoeaee?oei, ui aeicaiey? noai?eoe iiaeaeueio
nenoaio, ca aeiiiiiaith yei? iiaeia ye aea/aoe aieea ia ?icaeoie
i?aai?cio aaaaeaieo AeIE c iaaieie ooieoe?iiaeueieie ioeeaioeaeieie
iine?aeiaiinoyie, oae ? noaaeeoe ca aeieath naieo o?ainaai?a.

C iaoith noai?aiiy oaei? iiaeaeueii? nenoaie, o ceaioe ieo? oeyoii
i?e?i?i’?eoe?? aoei aaaaeaii ieaci?aeo pATV 8. Oey ieaci?aea
neeaaea?oueny c i?ia??onii? AeIE a??ono na?eiie ?aona eo?ae, ui i?noeoue
iaeei aeiaaee e?ioeaaee iiaoi?, yeo aoei eeiiiaaii ii naeoo aeey
?ano?eeoace Hind III o aaeoa??aeueiee aaeoi? pBR 322 [Katz R.A. et al.,
1982]. Aiae?c ?acoeueoao?a co?auaiiy o?ainaaiieo ieoae c ?ioaeoieie
iniaeiaie o??? ae naii? e?i?? iieacaa, ui /anoeia o?ainaaiieo ieoae
ia?aaeaaaea o?ainaai iauaaeeai ca iaiaeaeaanueeei oeiii oniaaeeoaaiiy,
ui na?ae/eei i?i ?ioaa?aoe?th aaaaeaii? ioeeaioeaeii? iine?aeiaiino?
a iaeio c o?iiinii ieo?. O ?ioi? /anoeie (noae?i?? A 1 oa A 6)
iaiaeaeaanueea oniaaeeoaaiiy aoei a?aenooi? — a?ae 80 aei 100% iauaaee?a
i?noeee o?ainaai. Oea aeicaieeei i?eionoeoe, ui a oeeo noae?i?yo
o?ainaai aai ?nioaaa o aeno?ao?iiiniiiiio noai?, aai aoa ?ioaa?iaaiee,
uiiaeiaioa, o aea? iaaiiieia?/i? o?iiiniie.

Aeine?aeaeaiiy noia?ii? AeIE c oaino?a o?ainaaiieo ieoae noae?i?e A 9 oa
A 14 oeyoii aeio-a?a?eaeecaoe?? c ?aae?iaeoeaii i?/aiith pATV 8
iieacaei, ui o ii??aiyii? c aaaaeaiith ieaci?aeith pATV 8, ui iaea
?ici?? aeecueei 14 o. i. i. oa i?noeea /ioe?e naeoe aeey ?ano?eeoace Eco
RI (?ici??e o?aaiaio?a: 7,7; 3,4; 2,3; 0,44 o. i. i.), o ??cieo
noae?i?yo o?ainaaiieo ieoae iieie?iiy F0 aeyaey?oueny ii iaeiiio
o?aaiaioo iaeiaeiaiai ?ici??o. O?aaiaioe iaee ?ici?? aeecueei 2,6 o. i.
i., ui ia a?aeiia?aea? aeiaeiiio c Eco RI-o?aaiaio?a pATV 8.
A?a?eaeecaoe?y noia?ii? AeIE ianooiieo iieie?iue o?ainaaiieo ieoae oeo
naieo noae?i?e, ?icuaieaii? Eco RI, c oei naiei ciiaeii, aeaea
aiaeia?/iee ?acoeueoao — iaeio nioao ?ici??ii aeecueei 2,6 o. i.
i.. A?aenooi?noue oa?aeoa?iiai iaoa?io ?ano?eeoe?? na?ae/eei i?i
ia?aaoaeiao no?oeoo?e aaaaeaii? ieaci?aee. ?ioaineai?noue neaiaeo aoea
i?eaeecii iaeiaeiaith o an?o c?aceao AeIE. Ii??aiyiiy c ?ioaineai?noth
neaiaeo iiceoeaiiai eiio?ieth aeicaieeei ioe?ieoe eii?ei?noue
o?ainaaio ye iaeio eii?th ia aeeiei?aeiee aaiii . O oie naiee
/an i/aaeaeii, ui ci?ieaoenue iaeii?aciai, no?oeoo?a o?ainaaio
noaa?e?coaaeany oa a?i ia?aaeaaaany ianooiiei iieie?iiyi o?ainaaiieo
ieoae aac ci?i. Aiaeia?/ia yaeua, eiee o?ainaai (aenieiiieiaaiiee
aaeaiia??on iaai SA 7) i?ney i?e?i?i’?eoe?? o ceaioo ieo?, ?ioaa?oaaa o
aaiii, caciath/e, iaeiae, i?e oeueiio ci?i o aeaeyae? aeaeaoe?e ie?aieo
ioeeaioeaeieo iine?aeiaiinoae, iienaia o ?iaio? [Eociaoeiaa A.Ae. e ae?.
1988]. Ci?ieaoenue o iieie?ii? F0, oeae o?ainaai oniaaeeiaoaaany
ianooiieie iieie?iiyie aac ci?i.

O? ae nai? o?eueo?e, ui aeei?enoiaoaaeeny aeey aeio-a?a?eaeecaoe?? o
iiia?aaeiueiio aenia?eiaio?, aoei a?aeieoi a?ae ciiaeo oa
ia?aa?a?eaeeciaaii c ?aae?iaeoeaii i?/aieie ?cieueiaaieie o?aaiaioaie
i?ia??onii? AeIE a??ono na?eiie ?aona, ye? i?noeee ie?ai? aaie
i?ia??ono. ?acoeueoao aeyaeany iaaaoeaiei o an?o aeiaaeeao. Oea
na?ae/eei i?i iiaio ae?i?iaoe?th i?ia??onieo iine?aeiaiinoae c
?ioaa?iaaiiai o?ainaaio. Iiae?aia ae?i?iaoe?y ioeeaioeaeieo
iine?aeiaiinoae o?ainaai?a cono??/a?oueny aeineoue /anoi. Oae, o
i?ia??oni?e AeIE a??ono na?eiie ?aona, yeo aoei ?i’?eiaaii o ceaioe
ieoae, a?aeaoaa?oueny ae?i?iaoe?y ie?aieo ae?eyiie oe??? AeIE [Aaca?yi
E.A. e ae?., 1985; Hu W.-S. et al., 1990]. I?ia??onia AeIE ?ioiai
?ao?ia??ono — a??ono eaeeai?? Iieii?, o o?ainaaiieo ieoao oaeiae
cacia? aeeaieeo ia?aaoaeia, o ia?oo /a?ao, aeiaaeo aeaeaoe?e
[Varela-Echavarria A. et al., 1993]. Aeaeaoe?? a??onii? AeIE aeyaeaii
oaeiae a ?ioaa?iaaiiio a??on? SV 40 o ee?oeiao ieoae [Gurney T. Jr. et
al., 1989].

Ca aeiiiiiaith aeaoaeueiiai aiae?co c aeei?enoaiiyi iaai?o ?ano?eeoac oa
aeio-a?a?eaeecaoe??, aaeaeiny anoaiiaeoe i?eaeeci? iaae? ?ioaa?iaaii?
ae?eyiee pATV 8 (?en. 1). ?ici?? ae?eyiee — aeecueei 1,5 o. i. i., oa
aiia ?ioaa?iaaia o Eco RI-o?aaiaio aaiiiii? AeIE ?ici??ii 1,1 o. i. i..
Iaeei c e?ioe?a ?ioaa?iaaii? ae?eyiee iaeaaeeoue aaio no?eeino? aei
aii?oeee?io, a ae?oaee — aaio-?ai?ani?o, ui iaiaaeo? eii?ei?noue pBR 322
o ee?oeiao E. coli. O neeaae? ae?eyiee caeeoeeany aaeoa??aeueia
noa?oiaa oi/ea ?aie?eaoe?? pBR 322. Iiaeia i?eionoeoe, ui /oaei??aeia
AeIE ia caoeuaia, iai?eeeaae, iaoeethaaiiyi, a?ae ae?? ioeeaac
?aoeei??ioa aai oi?io? no?oeoo?e, ye? ai?ciathoueny oeeie oa?iaioaie, ye
i?oai?. A?aeiii, ui e?eueoeaa? iieaeoee AeIE, i?ney aaaaeaiiy o
ca?iaeie, e?iaa?ecothoueny oacyenueeith ioeeaacith, i?ney /iai
a?aeaoaa?oueny aeaeaoe?y ie?aieo ae?eyiie [Eiceia A.I. e ae?., 1988]. C
?ioiai aieo, neeueiee i?iiioi?, ui i?noeoueny o aeiaaiio e?ioeaaiio
iiaoi?ia? a??ono na?eiie ?aona, iiaea neoaoaaoe oi/eith ?i?oe?aoe??
o?aine?eioe?? i?e aca?iiae?? c a?aeiia?aeieie oaeoi?aie
ee?oeie. Ne?ae a?aecia/eoe, ui aa?ieiaiiy aia??iiaeueiiai aaiiio ieo?
a?aeaoaa?oueny ia aeaiee?oeii?e noaae??, a ia iaeiiee?oeii?e a?eueo?noue
aai?a ?ai?aniaaii [Clegg K.B. et al., 1983]. ?acii c oei, ia
iaeiiee?oeii?e noaae?? aaiii ieo? noa? o?aine?eioe?eii ia?i?neaiei (oae
caaia, i?ii?ia aeoeaaoe?y aaiiio) [Schultz R.M., 1993]. Caaaeyee oeueiio
yaeuo, aaea ia i?ci?e iaeiiee?oeii?e noaae?? iiaea a?aeaoaaoeny
aeni?an?y o?ainaai?a [Ram P.T. et al., 1993; Kasuya M. et al., 1994;
Christians E. et al., 1995]. Oea aiai?eoue i?i oa, ui oaeoi?e, ye? ?
iaiao?aeieie aeey ?i?oe?aoe?? o?aine?eioe?? oa iiaeaeueoeo iiae?e,
i?enooi? o ceaioao ieoae aaea ia iaeiiee?oeii?e noaae??, oa eeoa
?ai?an?y i?iiioi??a aeieo? aeni?an?th aaiiio. Ia aeeeth/aii e oa, ui
aeni?an?y o?ainaai?a ia iaeiiee?oeii?e noaae?? iiynith?oueny
aea?ai?aniaaiei noaiii i?iiioi??a oa ?ioeo ?aaoeyoi?ieo iine?aeiaiinoae,
ye? aoiaeyoue aei ?oiueiai neeaaeo (oiaoi, a?aenooi?noth iaoeethaaiiy,
ca’yceo c? niaoeeo?/ieie a?eeaie, oiui). Naia oaea yaeua iiaei
a?aeaooeny o iaoiio aeiaaeeo, eiee neeueiee i?iiioi? (o neeaae? aeiaaiai
e?ioeaaiai iiaoi?o a??ono na?eiie ?aona), ui ciaoiaeeany o aeoeaiiio
noai?, iio?aiea aei ceaioe ieo?. Oo?i, ine?eueee ieoa ia ? ia?i?neaiei
oacy?i aeey a??ono na?eiie ?aona, iaa?oue o ?ac? ?i?oe?aoe??
o?aine?eioe?? ia a??oniiio i?iiioi??, aeiiaaoe?y iiaea ia a?aeaoaaoeny
(iaoai?ci ?i?oe?aoe?? ? a?eueo eiina?aaoeaiei, i?ae iaoai?ci
aeiiaaoe??). O oeueiio aeiaaeeo iaeiieaioethaiaa ae?eyiea, ui ooai?eeany
i?ae /an ?i?oe?aoe??, iiaea noaoe i?oaiith aeey ioeeaac, ye?
ooai?ththoue aeaieaioethaiaee ?ic?ea, a aeae? ?io? oa?iaioe
i?iaeiaaeothoue a?ae?ie?c a iaio iai?yieao. Coieieoeny oeae i?ioean
iiaea o i?noe? ciaoiaeaeaiiy aoi?eiieo no?oeoo? AeIE, ye? /anoi
ooai?ththoueny iiaoi?thaaieie iine?aeiaiinoyie.

?ioei iaoai?ciii, caeaoiei cae?enieoe ae?i?iaoe?th ie?aieo /anoei
o?ainaaio, ? aioo??oiuei- oa i?aeiieaeoey?ia ?aeiia?iaoe?y.
I?aeiieaeoey?ia ?aeiia?iaoe?y a?aeaoaa?oueny i?ae o?ainaaiii oa
o?iiiniiaie oacy?ia ia?aae eiai ?ioaa?aoe??th, a aioo??oiueiiieaeoey?ia
— i?ae ae?eyieaie iaiaaeaii? aiiieia?? ana?aaeei? o?ainaaio ia?aae aai
i?ney eiai ?ioaa?aoe?? o o?iiiniio oacy?ia. I?ia??oni? ioeeaioeaei?
iine?aeiaiino? a??ono na?eiie ?aona ? aaeueie ?aeiia?iaoe?eii aeoeaieie.
Aeiaaee e?ioeaaee iiaoi? a??ono i?noeoue “aa?y/? oi/ee” ?aeiia?iaoe??,
caeaoi? ?aeiia?ioaaoe c aiiieia?/ieie iine?aeiaiinoyie aiaeiaaiieo
?ao?iaeaiaio?a [Edelmann W. et al., 1989]. Iayai?noue caaaeaii?
aiiieia?? iiaea ni?eyoe ye ae?i?iaoe?? ie?aieo iine?aeiaiinoae
o?ainaaio, oae ? ?ioaa?aoe?? eiai o o?iiiniio a ?acoeueoao? aiiieia?/ii?
?aeiia?iaoe??. Aeoaea aaaeeeaei ? oie oaeo, ui i?ae /an aiae?co
o?ainaaiieo ieoae aoei aeyaeaii aea? noae?i?? (A 9 oa A 14), AeIE yeeo
aeaaaea i?e a?a?eaeecaoe?? nioae iaeiaeiaiai ?ici??o. Oea aiai?eoue i?i
iayai?noue niaoeeo?/ii? ?ioaa?aoe?? aeaiiai o?ainaaio.

?ioaa?aoe?y o?ainaaio a o?iiiniio iiaea a?aeaooeny oaeiae oeyoii
?aeiia?iaoe?? ii ae?eyieao neaaei? aiiieia?? i?ae ei?ioeeie iiaoi?aie,
ui aoiaeyoue aei eiai neeaaeo oa iiaoi?thaaieie iine?aeiaiinoyie aaiiio
ieo?. O oeueiio aeiaaeeo ia?aaoaeiae o?ainaaio iiaee a?aeaooeny
aiane?aeie ?ioaa?aoe?? o ianoaa?euei? iaeano? o?iiinii, ye? caciathoue
/anoeo ia?aaoaeia o i?ioean? aia??iaaiaco [Rudiger N.S. et al., 1995;
Gibbs M. et al., 1993]. Iaeiae, ia aeeeth/aii, ui ?ioaa?aoe?y a?aeaoeany
ii i?noeth aeaieaioethaiaiai ?ic?eao o?iiiniie oa ianooiiith
?aia?aoe??th iioeiaeaeaiiy. Oo?i, a oeueiio aeiaaeeo ne?ae i?eionoeoe
iayai?noue niaoeeo?/iino? ooai?aiiy oaeiai ?ic?eao oa nii??aeiaiino? aei
iueiai o?ainaaio, ine?eueee i?eiaeii? o aeaio noae?i?yo o?ainaaiieo
ieoae ?ioaa?aoe?y a?aeaoeany o oa ae naia i?noea o?iiiniie.

Ia aeeeth/aia iiaeeea?noue oiai, ui ia?aae noaa?e?caoe??th no?oeoo?e
o?ainaaio, a?i i?ieoia e?eueea ?aoiae?a ?ioaa?aoe??-aenoeec??, eiaeai c
yeeo noi?iaiaeaeoaaany ao?aoith /anoeie eiai ioeeaioeaeieo
iine?aeiaiinoae. ?acii c oei, i?ney an?o ia?aaoaeia, o?ainaai noaa
iano?eueee noaa?eueiei, ui ia ci?ithaaany ia i?ioyc? aaaaoueio
iieie?iue, oi/a aeineoue /anoi niinoa??aa?oueny iaioa noaa?euei?noue
o?ainaai?a — iinooiiaa ae?i?iaoe?y, aae aei iiaiiai cieeiaiiy c aaiiio
[Reik W. et al., 1985].

Iiaaae?iea o?ainaaio o iaoiio aeiaaeeo aiai?eoue i?i oa, ui: a)
ia?aaoaeiaa o?ainaaio a?aeaoeany ia ?aii?e noaae?? ?icaeoeo ca?iaeea,
ine?eueee o?ainaaii? ieo? ia aoee iica?eaie; a) c? neeaaeo o?ainaaio
ae?i?ioaaeeny an? ?aeiia?iaoe?eii aeoeai? oa a?aceea? ioeeaioeaei?
iine?aeiaiino?; a) ?ioaa?aoe?y o?ainaaio a?aeaoeany o noaa?eueio
ae?eyieo aaiiio. O ia?aaoaeiaao o?ainaaio a?aee o/anoue aeaa iaoai?cie:
a) ioeeaacia aeaa?aaeaoe?y, ?i?oe?eiaaia aeieeiaiiyi ?ic?ea?a o i?noeyo
ooai?aiiy iaeiieaioethaiaeo ae?eyiie oa a) ?aeiia?iaoe?y aiiieia?/ieo
iaeanoae oa ae?eyiie neaaei? aiiieia??.

No?oeoo?a aeno?ao?iiiniiiiai o?ainaaio ieo?.

O oie /an, ye o noae?i?yo A 9 oa A 14 o?ainaai ?ioaa?oaaa o aaiii ieo? o
e?eueeino? iaei??? eii?? oa niinoa??aaeiny eiai iaiaeae?anueea
oniaaeeoaaiiy, a noae?i?yo A 1 oa A 6 o?ainaai oniaaeeiaoaaee a?ae 80
aei 100% iauaaee?a o?ainaaiieo ieoae. Oea aoei iieacaii oeyoii
a?a?eaeecaoe?? noia?ieo AeIE, aeae?eaieo c oaino?a o?ainaaiieo ieoae oa
?icuaieaieo ?ano?eeoacith Eco RI, c ?aae?iaeoeaii i?/aiith pBR 322. I?e
oeueiio ?ici?? o?aaiaioo, ui a?a?eaeecoaaany, neeaaeaa aeecueei 5,5 o.
i. i.. C iaoith i?aeoaa?aeaeaiiy aeno?ao?iiiniiii? eieae?caoe??
o?ainaaio ie o?ainoi?ioaaee i?aia?aoaie noia?ii? AeIE c ia/?iee,
ueoiaeaeii? caeice, oeiono, iiceo oa oe??e o?ainaaiieo ieoae caaaeaieo
noae?i?e eiiiaoaioi? ee?oeie E. coli c ianooiiith naeaeoe??th ia
na?aaeiaeuao, ui i?noeee: a) aii?oe?e?i+oao?aoeeee?i; a) aii?oe?e?i; a)
oao?aoeeee?i. C AeIE noae?i?e A 1, A 5, A 6 aoei io?eiaii
o?ainoi?iaio?a, no?eeeo aei aii?oe?e?io, c A 4 — aei oao?aoeeee?io.
Iaeiae, eieii?? io?eiai? c AeIE noae?i?e A 4 oa A 5, ?inee iaaecae/aeii
iia?eueii iaa?oue ia aaaaoeo aeeaeeueieo na?aaeiaeuao, ui caaaaeeei
?oiueiio iai?aoethaaiith aeey aeine?aeaeaiiy oa caiaeii ?o aoei
ao?a/aii. Ia na?aaeiaeu?, ui i?noeei aii?oe?e?i+oao?aoeeee?i, ia aoei
io?eiaii aeiaeii? eieii?? c aeiaeii? noae?i??.

I?ia??onio AeIE a??ono na?eiie ?aona, ui aoiaeeea aei neeaaeo pATV 8,
aoei aaoaeiaaii o Hind III-naeo ?BR 322, ?icoaoiaaiee o 5`-e?ioe? aaio
no?eeino? aei oao?aoeeee?io, ui ?iaeoeaoaaei oeae aai. Oiio iiyaa o
e?eueeio noae?i?yo o?ainoi?iaio?a, no?eeeo aei oao?aoeeee?io, aiai?eoue
i?i iiaio ae?i?iaoe?th i?ia??onieo ioeeaioeaeieo iine?aeiaiinoae c?
neeaaeo o?ainaaia oa a?aeiiaeaiiy ooieoe?iiaeueiiai aaia. Oaea oi/ia
aenoeec?y o?ainaai?a oa a?aeiiaeaiiy aai?a, iioeiaeaeaieo o ?acoeueoao?
?ina?oe?eiiai iooaaaiaco, iieacaia o ?iaio? E. O. Aaca?yia oa
ni?a?ia?oiee?a [Aaca?yi E.A. e ae?., 1984]. I?e aaaaeaii? o ?aii?
aia??iie ae?icio?ee i?ia??onii? AeIE a??ono na?eiie ?aona, aaeaiia??ono
SA 7 oa ?ioeo AeIE, aoei iaea?aeaii oaiioeiia? iooaoe??. O iauaaee?a
o?ainaaiieo ioo oe? iooaoe?? cieeaee, oiaoi ooieoe?? iioeiaeaeaieo aai?a
a?aeiiaethaaeeny. I?e/eiith oaeiai oi/iiai “aeaeaeaiiy” /oaei??aeieo
ioeeaioeaeieo iine?aeiaiinoae c aaiii?a ?aoeei??io?a iiaea aooe
a?aenooi?noue o oeeo iine?aeiaiinoyo niaoeeo?/ieo neaiae?a, ui
caaacia/othoue iaoeethaaiiy oa, a?aeiia?aeii, noaa?euei?noue
(iai?eeeaae, no?ee?noue aei ioeeaac) ioi/oth/eo ae?eyiie aaiiio.

Aeaoaeueii aoei aeine?aeaeaii ieaci?aeo, “a?yoiaaio” c AeIE noae?i?? A
6. Oeth ieaci?aeo aoei iacaaii ?A6.5, ca iiia?ii o??? ieo?, c yei?
?? aoei aia?oa aeae?eaii. I?e aoaeoeaiino? o?ainoi?iaoe?? 1o107
eieii?e/iea AeIE, aeey iaeiaeiaeo e?eueeinoae noia?ii? AeIE aoei
iaea?aeaii oae? ?acoeueoaoe: AeIE oe??e — 40 eieii?e, AeIE ia/?iee — 12
eieii?e, AeIE oeiono — 135 eieii?e, no?eeeo aei aii?oe?e?io. I?ney
?iciiiaeaiiy eieii?e oa aeae?eaiiy c ieo ieaci?aeieo AeIE, aoei
anoaiiaeaii ?aeaioe/i?noue ?oi?o ?ici???a oa iaoa?i?a ?ano?eeoe?? o an?o
aeine?aeaeaieo i?aaiao. Iauaaeee ieo? A6.5 oaeiae i?noeee
aeno?ao?iiiniiiee o?ainaai, yeee aeyaeany ?aeaioe/iei oiio, ui aoa
aeae?eaiee c ieo? iieie?iiy F 0 [*aueia E.E. e ae?., 1997]. Oie oaeo, ui
aeaiee o?ainaai aoa caeaoiee aei ?aie?eaoe?? o aaeoa??aeueieo ee?oeiao,
na?ae/ea i?i iayai?noue o eiai neeaae?, ii?yae c aaiii no?eeino? aei
aii?oe?e?io, aaeoa??aeueii? noa?oiai? oi/ee ?aie?eaoe??.

Ieaci?aea ?A6.5, aeae?eaia c o?ainaaiieo ieoae, iaea ?ici?? aeecueei 5,5
o. i. i., oiae? ye pATV 8, yeo aoei aaaaeaii — 14 o. i. i.. Oea aiai?eei
i?i oa, ui o oiae? aca?iiae?? c aaiiiii ieo? o?ainaai caciaa aeeaieeo
ia?aaoaeia, iia’ycaieo c aeaeaoe??th aeiaaeo ae?eyiie. Aeey eieae?caoe??
aeaeaoiaaieo ioeeaioeaeieo iine?aeiaiinoae aoei iiaoaeiaaii o?ce/io
ea?oo o?ainaaio (?en. 2). Ne?ae a?aecia/eoe, ui i?ae /an
aeio-a?a?eaeecaoe?? noia?ii? AeIE o?ainaaiieo ieoae noae?i?? A 6,
?icuaieaii? ?ano?eeoacith Eco RI, aoei aeyaeaii o?aaiaio ?ici??ii 5,5 o.
i. i., ui a?a?eaeecoaaany. Oaeei naiei aoa ? aecia/aiee i?ci?oa ?ici??
aeno?ao?iiiniiiiai o?ainaaio, oiaoi, aeai? a?a?eaeecaoe?? na?ae/eee i?i
iayai?noue o o?ainaai? iaeiiai naeoo aeey Eco RI. Iaeiae, i?ae /an
aeaoaeueiiai ea?ooaaiiy ieaci?aee ?A6.5, iai?aoeueiaaii? o ee?oeiao E.
coli, aeyaeeiny, ui aiia i?noeoue aeaa naeoe aeey Eco RI. Eiia??i?noue
iiaeeo?eaoe?? o?ainaaio i?ae /an ?iciiiaeaiiy o aaeoa??aeueieo ee?oeiao
aeeeth/aia, ine?eueee aiaeia?/i? ?acoeueoaoe io?eiaii a e?eueeio
eiio?ieueieo aenia?eiaioao. Oeae oaeo “?acenoaioiino?” iaeiiai c Eco
RI-naeo?a o?ainaaio, nei??o ca ana, na?ae/eoue i?i iayai?noue
niaoeeo?/iiai iaoeethaaiiy oeueiai naeoo, aai, oaeaeoa, oe?ei? iaeano?
o?ainaaio, aea a?i ciaoiaeeoueny. I?ney ?iciiiaeaiiy o ee?oeiao
aaeoa??e, iaoa?i iaoeethaaiiy, oa?aeoa?iee aeey ee?oei ieo?, ia
caa??any. “*anoeiaa” iaoeethaaiiy o?ainaaio iiaea aooe aeeeeeaia
iayai?noth o iueiio niaoeeo?/ieo neaiae?a ai?ciaaaiiy aeey aoea??ioe/ieo
iaoeeac.

Ei?enooth/enue o?ce/iith ea?oith, ie eeiioaaee oa n?eaaioaaee o?aaiaioe
?A6.5, ui caiaeii aeicaieeei aecia/eoe iiaio ioeeaioeaeio
iine?aeiai?noue o?ainaaio, ooi/ieoe o?ce/io ea?oo oa iaea?aeaoe a?aieoe?
aeaeaoiaaieo ae?eyiie. Aeyaeeiny, ui aeaeaoe?? oi?eioeeny, aieiaiei
/eiii, i?ia??onii? AeIE a??ono na?eiie ?aona. Ia a?aei?io a?ae
?ioaa?iaaiiai o?ainaaio, aea i?ia??onia AeIE aoea iiai?noth ae?i?iiaaia,
a aeno?ao?iiiniiiiio o?ainaai? caeeoeany ?? o?aaiaio ?ici??ii aeecueei
2850 i. i.. A?i ai?uo? iaeaea iiaio iine?aeiai?noue aaio env oa
i?aeaaiio iaeanoue aai?a env oa src. Iaeei c e?ioe?a o?aaiaioo
ciaoiaeeoueny ana?aaeei? eiaeoth/i? iaeano? aaio env, ca 110 i. i. i?ney
noa?oiai? oi/ee o?aine?eioe?? oeueiai aaio, a ae?oaee — o i?aeaaii?e
iaeano? aai?a env oa src, ca 170 i. i. i?ney cae?i/aiiy eiaeoth/i?
iine?aeiaiino? aaio env oa ia?aae ii/aoeii iaeiaeoth/i? iine?aeiaiino?
aaio src. Ia iaio e?ioeyo o?aaiaioo iiaeeco eiai a?aieoeue ? ei?ioe?
AO-aaaao? ae?eyiee. O aeo?aei?e ieaci?ae? pATV 8 i?ia??onio AeIE aoei
eeiiiaaii o Hind III-naeo aaeoi?a pBR 322. O ?A6.5, e??i ae?i?iaoe??
a?eueoi? /anoeie i?ia??onieo iine?aeiaiinoae, o?aaiaio, ui caeeoeany,
aoa o?ainiiciaaiee a ?ioo, ?icoaoiaaio iiaeeco ae?eyieo aaeoi?a, i?e/iio
naeo aeey Hind ??? a?aeiiaeany. O?ainiiceoe?y a?aeaoeany o 5`-e?iaoeue
aaio no?eeino? aei oao?aoeeee?io pBR 322, i?e oeueiio aeaeaooaaeany
ei?ioea ae?eyiea oeueiai aaio aeiaaeeiith aeecueei 100 i. i.. Oey
ae?eyiea oaeiae aoea oeaieiaaia ei?ioeeie AO-aaaaoeie ioeeaioeaeieie
iine?aeiaiinoyie. Ae?oaa aeaeaoe?y aaeoa??aeueii? /anoeie pATV 8 iaea
?ici?? 560 i. i. oa oi?eioeany aaio-?ai?ani?o, yeee iaiaaeo? eii?ei?noue
ieaci?aee o ee?oeiao E. coli. Aeaeaoiaaiee o?aaiaio aoa iaiaaeaiee
ae?eyieaie, aaaaoeie A ? O. A?aieoe? aeaeaoe?e oa o?ainiiceoe?e, a
oaeiae iiaiooai?ai? ae?eyiee, iieacaii ia ?en. 3. Oe?eaaei ? oa, ui naia
o ?aeii? aaio-?ai?ani?o ciaoiaeeoueny iaeia c a?aieoeue ?ioaa?iaaiiai
o?ainaaio. Iiaeeeai, i?e/eiith oeueiai aoee no?oeoo?i? iniaeeaino?
aeaii? iaeano?. Acaaae?, caa?oa? ia naaa oaaao oie oaeo, ui an? iaeano?
aeno?ao?iiiniiiiai o?ainaaio, ye? caciaee ia?aaoaeia, aoee oeaieiaaieie
AO-aaaaoeie ae?eyieaie. A ca’yceo c oeei, caea?oueny eiia??iei, ui oae?
ae?eyiee noaee i?oaiyie aeey niaoeeo?/ieo ?aeiia?iaoe?eieo iaoai?ci?a,
ye? ae?thoue ia ?aii?o noaae?yo aia??iaaiaco ieo?. Oea iiaeooue aooe ye
niaaeeia? ia?aaoaeiae aaiiio, oae ? aiie?o?eaoe?y /e i?aeaioiaea aei
aiie?o?eaoe?? ie?aieo ioeeaioeaeieo iine?aeiaiinoae. Ine?eueee
?aeaioe/i? iieaeoee o?ainaaio i?noeeeny o an?o aeine?aeaeaieo i?aaiao
o?ainaaiieo ieoae noae?i?? A 6, ieo? ia aoee iica?eaie, oiaoi,
ia?aaoaeiae o?ainaaio a?aeaoeeny o ?aiiueiio ca?iaeeo. Ia?aaoaeiae
aeno?ao?iiiniiiiai o?ainaaio iiaee a?aeaooeny ye o ?acoeueoao?
?ioaa?aoe?? o ianoaa?eueio aai caeaoio aei aiie?o?eaoe?? iaeanoue aaiiio
oa ianooiii? aenoeec??, oae ? o iicao?iiiniiiiio noai?, i?e
?aeiia?iaoe?? c aeno?ao?iiiniiieie AeIE ieo?. Iiaeeea?noue oaei?
?aeiia?iaoe?? iieacaia o iiaeaeueieo nenoaiao o ee?oeiao ieoae [Hellgren
D. 1992; Lin F.-L.M. et al., 1987] oa ?ioeo i?aai?ci?a [Puchta H. et
al., 1992]. Oe?eaai, ui iaeia c a?aieoeue aeaeaoe?e iaeaea caaaeaee
ciaoiaeeeany o 5`-e?ioe? aaio env. Ine?eueee o ieaci?ae? ?A6.5,
yeo ie aeine?aeaeoaaee, iaeia c a?aieoeue aeaeaoiaaiiai o?aaiaioo
eaaeeoue naia o 5`-iaeano? aaio env, iiaeia i?eionoeoe, ui ioeeaioeaei?
iine?aeiaiino?, ye? ciaoiaeyoueny o oeueiio i?noe?, ? “aa?y/eie oi/eaie”
?aeiia?iaoe??.

Oae naii, ye ? o aeiaaeeo ?ioaa?iaaiiai o?ainaaio, o aeaio noae?i?yo
o?ainaaiieo ieoae (A 1 oa A 6) c iaiaiaeae?anueeei oniaaeeoaaiiyi
o?ainaaio, aoei aeyaeaii o?aaiaioe iaeiaeiaiai ?ici??o, ui
a?a?eaeecoaaeeny. Oi/a caiaeii aeaoaeueii aoei aeine?aeaeaii
aeno?ao?iiiniiiee o?ainaai o?eueee c noae?i?? A 6, iiaeia i?eionoeoe, ui
a iaio noae?i?yo o?ainaaie aoee iaeiaeiaeie. Oa, ui iaeiaeia? aeciaaii?
AeIE iiaeooue iaaeae? ?nioaaoe ye o aeno?ao?iiiniiiiio, oae ?
?ioaa?iaaiiio noaiao, iiaeia iiynieoe ii-??ciiio. Ii-ia?oa, oea
iiaea aooe iane?aeeii ?ioaa?aoe?? o ??ci? ae?eyiee aaiiio,
?acoeueoaoii /iai noathoue ??ci? ia?aaoaeiae o?ainaaio oa, a?aeiia?aeii,
caeaoi?noue /e iacaeaoi?noue aei ianooiii? aenoeec?? oa
aeno?ao?iiiniiiiai ?nioaaiiy. Ii-ae?oaa, yeui ia?aaoaeiae o?ainaaio
a?aeaoaathoueny o iicao?iiiniiiiio noai?, noa? iiaeeeaith
naeaeoe?y ieaci?ae, caeaoieo aei aaoiiiiii? ?aie?eaoe??, iai?eeeaae,
?acii c i?e?iaeieie aeno?ao?iiiniiieie AeIE. Ii-o?ao?, ??ci?
i?e/eie iiaeooue iaoiiaeoe ia?aaoaeiao o?ainaai?a ia ie?aieo aoaiao
?aiiueiai ?icaeoeo ca?iaeea, ui aieeia ia oa?aeoa? ia?aaoaeia oa ia
oi?io ?nioaaiiy o?ainaaio. Oaeith i?e/eiith iiaea aooe iaiaeii/ani?noue
aaaaeaiiy aeciaaiii? AeIE i?e ?iaio? c aaeeeith e?euee?noth ca?iaee?a.
Iaa?oue ca iaaaeeeee i?ii?aeie /ano o ceaio? iiaeooue a?aeaooeny
i?ioeane, ui noaiooue ae??oaeueieie aeey aeie? o?ainaaio, oae? ye
ia?ao?ae a?ae ?ai?aniaaiiai noaio aaiiio aei eiai i?ii?ii? aeoeaaoe?? o
i?ci?e G2-oac?.

?aie?eaoe?y oa naa?aaaoe?y aeno?ao?iiiniiiiai o?ainaaio ieo?.

Ui noino?oueny aoaeoeaii? aeno?ao?iiiniiii? ?aie?eaoe?? oa naa?aaaoe??,
oi ?o caaacia/oaaee, nei??o ca ana, ooai?ai? de novo aiane?aeie
ia?aaoaeia eiia?iaoe?? a??onii? oa aaeoa??aeueii? neeaaeiaeo o?ainaaio.
Ia iiaeia aeeeth/aoe iiaeeea?noue ? oiai, ui o aeeiiaii? oeeo ooieoe?e
a?aee o/anoue aaeoa??aeuei? ioeeaioeaei? iine?aeiaiino?,
a?aeiia?aeaeuei? ca oe? i?ioeane o ee?oeiao E. coli. I?ae /an aea/aiiy
ori ?aie?eaoe?? ??cieo aoea??io?a, aoei aeyaeaii eiina?aaoeaio AO-aaaaoo
eaiii?/io iine?aeiai?noue [Donovan S. et al., 1996]. Aiae?c
ioeeaioeaeii? iine?aeiaiino? ?A6.5 iieacaa, ui iiaiooai?ai? a
?acoeueoao? aeaeaoe?e oa o?ainiiceoe?e iine?aeiaiino? (iaaaaeath, ui
a?aieoe? ia?aaoaeia o?ainaaio i?noyoue AO-aaaao? ae?eyiee), iathoue
aenieo aiiieia?th c eaiii?/iith iine?aeiai?noth ori ?aie?eaoe??
aoea??io?a (?en. 4). Oaeei /eiii, iiaeia i?eionoeoe, ui noaa?eueia
aeno?ao?iiiniiia ?aie?eaoe?y o?ainaaio o ee?oeiao ieo? cae?enithaaeany
caaaeyee oeei ioeeaioeaeiei iine?aeiaiinoyi. Oae? oaeoe, eiee
?aie?eaoe?th ?i?oe?aoe?ththoue, e??i ori, ?io? ae?eyiee AeIE, a?aeii?.
Eeiiiaai? iiaoi?e ?iaeeie Alu I, caaacia/oaaee ?aie?eaoe?th ieaci?ae, ui
?o i?noyoue, o aoea??ioe/ieo ee?oeiao [Johnson E.M. et al., 1986]. E??i
oiai, a?aecia/aii, ui ori ?aie?eaoe?? pBR 322 ai?cia?oueny
aoea??ioe/ieie oaeoi?aie ?aie?eaoe?? [Goldberg E. Z., 1981].

Aaaeeeaa cia/aiiy iiaea iaoe oie oaeo, ui o aaeoa??aeuei?e /anoei?
o?ainaaio a?aeaoeany aeaeaoe?y iaeano?, ui i?noeea aai, yeee iaiaaeo?
eii?ei?noue pBR 322 o ee?oeiao E. coli. A?oia aeine?aeiee?a i?ae
ea??aieoeoaii M. Botchan iieacaea, ui naia oey ae?eyiea pBR 322 (i?ae
aaeoa??aeueiith noa?oiaith oi/eith ?aie?eaoe?? oa naeoii aeey
?ano?eeoace Pvu II) i?noeoue oeen-ae?th/o iine?aeiai?noue, yea ?noioii
?ia?ao? ?aie?eaoe?th a??ono SV 40, eeiiiaaiiai o pBR 322, o ia?i?neaieo
ee?oeiao [Lusky M. et al., 1981].

Oaeei /eiii, caeaoi?noue ?A6.5 aei aeno?ao?iiiniiii? ?aie?eaoe??
coiiaeaia: a) noiae?noth iiaiooai?aieo o ?acoeueoao? ia?aaoaeia
o?ainaaio aai aeayeeo aaeoa??aeueieo iine?aeiaiinoae c iine?aeiaiinoyie
ori ?aie?eaoe?? aoea??io?a; a) ao?aoith aaeoa??aeueiiai aaio-iaiaaeoaa/a
eii?eiino?; a) iayai?noth o neeaae? ?A6.5 o?aaiaioo i?ia??onii? AeIE
a??ono na?eiie ?aona, ui i?noeoue i?aeaaiio iaeanoue aai?a env oa src.

Naa?aaaoe?y o?ainaaia iiaea a?aeaoaaoeny ca o?iiiniiiei iaoai?ciii, yeui
eiai iine?aeiaiino? acyee ia naaa ooieoe?? oeaio?iia?, aea a?eueo
eiia??iei caea?oueny iaeei c aeaio ?ioeo iaoai?ci?a naa?aaaoe??. Ia?oee
c ieo — oea ?icae?e iieaeoe o?ainaaio i?ae aei/??i?ie yae?aie o i?ioean?
ee?oeiiiai iiae?eo. Ia ei?enoue oaeiai oeyoo naa?aaaoe?? aiai?eoue iaeee
?ici?? ?A6.5, yeee aeicaiey? ieaci?ae? oaeaeei ?aie?eoaaoeny oa
iaeiie/oaaoeny o ee?oei? o aaeee?e e?eueeino?. I?e oeueiio, a aei/??i?
ee?oeie i?ae /an iiae?eo iio?aiey? i?eaeecii iaeiaeiaa e?euee?noue
o?ainaaio.

Ae?oaee eiia??iee iaoai?ci naa?aaaoe?? o?ainaaio — oea ?iciiae?e ca
i?eioeeiii, ui aeei?enoiao?oueny i?e ?icae?e? iecueeieii?eieo ieaci?ae o
i?iea??io?a. Oae? ieaci?aee eiaeothoue a?eee, ye? ca’ycothoueny c
iaaieie iine?aeiaiinoyie oeeo ieaci?ae, oa iaeii/anii, c aaeoa??aeueiith
o?iiiniiith. I?ae /an ee?oeiiiai iiae?eo ieaci?aee ?icoiaeyoueny o
aei/??i? ee?oeie ?acii c aei/??i?ie o?iiiniiaie, a iio?i
a?ae’?aeiothoueny a?ae ieo. ?A6.5 ia eiaeo? iiae?aieo a?ee?a, iaeiae,
iiaeia i?eionoeoe, ui o ee?oeiao ieo? ? ?io? AeIE-ca’ycoth/? a?eee c
aeaiia oeaio?aie ca’ycoaaiiy. Oae? a?eee, anioeeeiaai? c o?iiiniiaie
ieo?, iiaeooue i?e?aeioaaoe ?A6.5 oa ia?aiineoe ?? o aei/??i? ee?oeie
i?ae /an ee?oeiiiai iiae?eo.

?aie?eaoe?y aeno?ao?iiiniiiiai o?ainaaio ieo? o ee?oeiao eiiao

A?aoiaoth/e oie oaeo, ui eaiii?/ia iine?aeiai?noue ori ?aie?eaoe??
aoea??io?a ? eiina?aaoeaiith o an?o aoea??io?a, ie ae??oeee ia?aa??eoe
caeaoi?noue aeno?ao?iiiniiiiai o?ainaaio ieo? ?aie?eoaaoeny o ee?oeiao
?ioeo aoea??io?a. Aeey oeueiai ie aaaee ?A6.5 o ee?oeie eiiao e?i?? SF
21. A yeino? eiio?ieth o o?ae nai? ee?oeie aaiaeeeeny ieaci?aee pBR 322
oa pATV 8. A aenia?eiaioao A. C. Oa?aiooea ?aeiia?iaioia ieaci?aea, ui
neeaaeaeany c pBR 322 oa aeiaaiai e?ioeaaiai iiaoi?a a??ono na?eiie
?aona, i?e i?e?i?i’?eoe?? o a?aio oooiaiai oiaeii?yaeo, iaaoea
caeaoiino? aei aeno?ao?iiiniiii? ?aie?eaoe?? aiane?aeie ?ina?oe??
o?aaiaioo aaiiiii? AeIE eiiaoe [Ieeieaaa A.E. e ae?., 1992].

O?eaae?noue ee?oeiiiai oeeeeo o ee?oei e?i?? SF 21 noaiiaeoue aeecueei
iaei??? aeiae, oiio ?aie?eaoeaia iiaaae?iea ieaci?ae aeine?aeaeoaaeany
ia 2-e, 3-e, 4-e oa 7-e aei? i?ney o?ainoaeoe??. Aeey oeueiai ae?eaioe
ee?oeiieo eoeueoo?, ui i?noeee iaeiaeia? e?eueeino? ee?oei (3o105),
iaiineee ia iaeeiiiaee o?eueo?, e?coaaee oa a?a?eaeecoaaee c
?aae?iaeoeaii i?/aiith pBR 322 (?en. 5A). Ia aeaineoia?ai? (?en. 5A)
aeaeii, ui ai?no ?A6.5 o ee?oeiao caeeoa?oueny iaeaea iaeiaeiaei ia 2-e,
3-e oa 4-e aei?, ui aiai?eoue i?i ?aie?eaoe?th ieaci?aee. Ine?eueee ia
eiaeiiio aoai? aiae?coaaeeny iaeiaeia? e?eueeino? ee?oei, i?e
a?aenooiino? ?aie?eaoe?? niinoa??aaeiny a i?ia?anoth/a cieaeaiiy ai?noo
o?ainaaio i?ney eiaeiiai ee?oeiiiai iiae?eo. A?aoiaoth/e oie oaeo, ui o
iiioeyoe?? ee?oei ii?yae c oeie, ui i?noeee o?ainaai, aoee ?
iao?aino?eiaai?, iiaeia c?iaeoe aeniiaie, ui ?aie?eaoe?y a?aeaoaa?oueny
aeineoue aeoeaii oa o?ainaai ia i?eai?/o? aeeoo?ae?yeuei?noue ee?oeie oa
ia aieeaa? ia o?eaae?noue ee?oeiiiai oeeeeo. Ia 7-e aeaiue
a?aeaoaa?oueny aeaa?aaeaoe?y eoeueoo?e ee?oei. Ai?no eiio?ieueieo
ieaci?ae pBR 322 oa pATV 8 aaea ia 2-e aeaiue aoa iii?oii iaioei, i?ae
?A6.5 (oi/a aeey o?ainoaeoe?? aeei?enoiaoaaeeny iaeiaeia? e?eueeino?
ieaci?aeieo AeIE), a aeae? ??cei cieaeoaaeiny, aae aei iiaiiai cieeiaiiy
ia 4-e aeaiue. Oea na?ae/eoue i?i a?aenooi?noue ?aie?eaoe?? oa
ei?ioei/ania “ia?aaeeaaiiy” ieaci?ae o ee?oeiao. Aeey anoaiiaeaiiy
oaeoo naia iicao?iiiniiii? ?aie?eaoe?? ?A6.5 o ee?oeiao eiiao, c ieo
aoei aeae?eaii noia?io AeIE. Oey AeIE aeei?enoiaoaaeany aeey
o?ainoi?iaoe?? eiiiaoaioieo ee?oei E. coli ooaiio DH 5 c ianooiiith
naeaeoe??th ia na?aaeiaeu?, ui i?noeei aii?oeee?i, a ?acoeueoao? /iai
aoei io?eiaii eieii??, ye? i?noeee ieaci?aeo, ui aoea ?aeaioe/iith
?A6.5. Oea aoei iieacaii oeyoii ii??aiyiiy ?ano?eeoe?eieo iaoa?i?a aeaio
ieaci?ae i?e ?icuaieaii? ?ano?eeoacaie Bgl II, Eco RI, Hind III oa Pst
I. Oeae oaeo aiai?eoue i?i a?aenooi?noue ia?aaoaeia o?ainaaio i?ae /an
?aie?eaoe?? o ee?oeiao iiaiai oacy?ia. Ae?oao /anoeio noia?ii? AeIE
ee?oei, o?aino?eiaaieo ?A6.5, aoei aeei?enoaii aac ?icuaieaiiy
?ano?eeoacaie aeey i?iaaaeaiiy aeio-a?a?eaeecaoe?? c oei naiei ciiaeii,
ui aeei?enoiaoaaany aeey aeio-a?a?eaeecaoe?? (?en. 5A). Aeaeii, ui
?ici??e nioa, ye? a?a?eaeecothoueny o noia?ieo AeIE, a?aeiia?aeathoue
oaeei o eiio?ieth — ieaci?aee pA6.5. Aaiiii? AeIE o?aino?eiaaieo ee?oei
ia a?a?eaeecothoueny, ui na?ae/eoue i?i a?aenooi?noue ?ioaa?aoe??
o?ainaaio o aaiii oacy?ia.

Oa, ui ioeeaioeaei? iine?aeiaiino?, ye? caaacia/othoue ?aie?eaoe?th
aeno?ao?iiiniiiiai o?ainaaio o ee?oeiao ieo?, caa??aathoue nai? ooieoe??
oaeiae o ee?oeiao aaiethoe?eii aeaeaeeo i?aai?ci?a, i?aeoaa?aeaeo?
aenieo eiina?aaoeai?noue ?aie?eaoe?eiiai aia?aoo aoea??io?a. Ne?ae
a?aecia/eoe, ui ine?eueee aeiaeia c eiio?ieueieo ieaci?ae ia aoea
caeaoiith ?aie?eoaaoeny o ee?oeiao eiiao, ioeeaioeaei? iine?aeiaiino?,
ye? aoiaeyoue aei ?oiueiai neeaaeo, ia iiaeooue ooieoe?iioaaoe ye ori
?aie?eaoe?? a oeeo ee?oeiao. Oiio iiaeia c?iaeoe aeniiaie, ui o ?A6.5
oey ooieoe?y aeeiio?oueny iiaiooai?aieie o ?acoeueoao? ia?aaoaeia
o?ainaaio iine?aeiaiinoyie AeIE. Ia iniia? oeueiai iiaeia i?eionoeoe, ui
? o ee?oeiao ieo? naia iiaiooai?ai? ioeeaioeaei? iine?aeiaiino? aoee
oi/eaie ?i?oe?aoe?? ?aie?eaoe?? o?ainaaio. Oie oaeo, ui o ee?oeiao eiiao
?A6.5 ia caciaaaea ia?aaoaeia oa ia ?ioaa?oaaea o aaiii, ?nioth/e
aeno?ao?iiiniiii, aiai?eoue i?i noaa?euei?noue no?oeoo?e o?ainaaio oa
eiai iecueeo ?aeiia?iaoe?eio aeoeai?noue. Oae? aeanoeaino?, ye noae?
?aie?eaoe?y oa naa?aaaoe?y, noaa?euei?noue no?oeoo?e, a?aenooi?noue
aieeao ia aeeoo?ae?yeuei?noue ee?oei, aeicaieythoue o iaeaooiueiio
aeei?enoiaoaaoe ?A6.5 aeey noai?aiiy aeno?ao?iiiniiieo aoea??ioe/ieo
aaeoi??a c oe?ieei eieii oacy?a.

OAIIOEIIA? I?IssAE O?AINAAIICO

Aiae?c ?acoeueoao?a co?auaiue o?ainaaiieo ieoae c ii?iaeueieie ieoaie
o??? naii? e?i?? na?ae/eoue i?i iayai?noue ii?ooaiue ?ai?iaeoeoeaii?
caeaoiino? o ?yaeo o?ainaaiieo naiie, ye? i?noeee ioeeaioeaei?
iine?aeiaiino? pATV 8. A?ae iaei??? naiee acaaae? ia aaeaeiny io?eiaoe
iauaaee?a o co?auaiiyo c naioeyie, yeeo ia?aae oeei aoei ia?aa??aii o
?ioeo co?auaiiyo. Cia/ia cieaeaiiy ?ai?iaeoeoeaii? caeaoiino?
a?aecia/aii oaeiae o iieie?ii? F1 o?ainaaiieo naiie. O iaei?e c noae?i?e
na?aae iauaaee?a ae?oaiai oa o?aoueiai iieie?iue aeyaeaii
ia?oaiia?iaeaeaieo ieoaiyo c iaaei?icaeiooeie oainoaie. I?e ii??aiyii?
ieoae iaeiaeiaeo a?eiaeo a?oi aeyaeaii, ui /anoioa aeieeiaiiy iooeei o
o?ainaaiieo naiie ?noioii ia?aaeuo? eiio?ieueiee ??aaiue.

O o?ainaaiieo oaa?ei iaio noaoae o a?oe? a?ae oanoe i?nyoe?a aoei
aeyaeaii aeaoaeoe oe??e, aeiaaeaiiy oa?no?, aaeee? ae?acee ia o?e? oa
aooao, ii?ooaiiy eii?aeeiaoe?? ?oo?a — iae?iiao??. *anoioa i?iya?a oeeo
iciae ci?ithaaeany o ianooiieo iieie?iiyo, aea aiie aeyaeyeeny iino?eii.
E??i oiai, o iieie?iiyo F3-F5 a?aecia/aii iiyao ieoae c oaiiiaiii
“iie?i?” oa?no?, ui ?noioii a?aenoaaaee o aac? a?ae ii?iaeueieo oaa?ei.

Ae?i?iaoe?y on?o i?ia??onieo oa cia/ii? /anoeie aaeoa??aeueieo
ioeeaioeaeieo iine?aeiaiinoae o aeiaaeeo ?ioaa?iaaiiai o?ainaaio, ia
aeicaiey? iia’ycaoe oaiioeiia? aoaeoe c aeni?an??th aai?a, ui aoiaeeee
aei eiai neeaaeo. Ioaea, a?ieia?/i? aoaeoe ?ioaa?iaaiiai o?ainaaio
coiiaeai?, nei??o ca ana, oei, ui i?ae /an ?ioaa?aoe?? o?ainaai ii?ooea
?aaoeyoe?th aai ?iaeoeaoaaa iaaiee aai (aai aaie i?e iayaiino? e?eueeio
?aoiae?a ?ioaa?aoe??/aenoeec??). Aiaeia?/iee iaoai?ci i?a ae?yoe ? o
aeiaaeeo aeno?ao?iiiniiiiai o?ainaaio, yeui eiai noaa?e?caoe?? o
aeno?ao?iiiniiiiio noai? ia?aaeoaaea ?ioaa?aoe?y o aaiii oa ianooiia
aenoeec?y.

Oie oaeo, ui o aeayeeo noae?i?yo o?ainaaiieo ieoae /anoioa i?iyao
aoaeo?a ci?ithaaeany, a oaeiae c’yaeyeeny iia? oaiioeiia? iciaee
(“iie?a” oa?noue oa aeao?oeeo aaae o ieoae a iieie?iiyo F3-F5),
aiai?eoue i?i oa, ui o oeeo noae?i?yo o?ainaai i?a ?ioaa?oaaoe o
ianoaa?euei? iaeano? o?iiinii oa aiane?aeie oeueiai a?aeaoaaeeny eiai
o?ainiiceoe?? o iia? ae?eyiee aaiiio. I?e oeueiio iine?aeiaiino?
o?ainaaio caa??aaeeny oa aieeaaee ia o?ainaaiieo ieoae, aeeeeeath/e
iiyao oaiioeiiaeo aoaeo?a.

AENIIAEE

1. I?e i?e?i?i’?eoe?? o ceaioe ieo? ieaci?aee pATV 8 /anoeia io?eiaieo
o?ainaaiieo ieoae i?noeoue o?ainaai a aeno?ao?iiiniii?e, a /anoeia — a
?ioaa?iaai?e o aaiii oi?i?. Iaeaea? oi?ie o?ainaaio caciaee aeeaieeo
ia?aaoaeia o ii??aiyii? c aaaaeaiith ieaci?aeith.

2. I?ae /an ?ioaa?aoe?? c? neeaaeo o?ainaaio ae?i?ioaaeany any
i?ia??onia AeIE a??ono na?eiie ?aona oa a?eueoa /anoeia aaeoa??aeueieo
iine?aeiaiinoae.

3. O neeaae? aeno?ao?iiiniiiiai o?ainaaio caeeoeeany iaeaea iiaia
iine?aeiai?noue aaio env, yea aoea o?ainiiciaaia c naeoo eeiioaaiiy o
?icoaoiaaio iiaeeco ae?eyieo iieaeoee. ?aie?eaoe?y aeno?ao?iiiniiiiai
o?ainaaio caaacia/oaaeany iiaiooai?aieie o ?acoeueoao? eiai ia?aaoaeia
ioeeaioeaeieie iine?aeiaiinoyie.

4. sse aeno?ao?iiiniiiee, oae ? ?ioaa?iaaiee o?ainaaie noaa?eueii
oniaaeeiaothoueny aac iiaeaeueoi? ci?ie no?oeoo?e oa aeyaeythoueny o
an?o oeaieiao ? i?aaiao.

5. Aiae?c no?oeoo?e o?ainaaio na?ae/eoue ia ei?enoue oiai, ui o eiai
ia?aaoaeiaao a?aee o/anoue ?aeiia?iaoe?ei? iaoai?cie oa ioeeaacia
aeaa?aaeaoe?y. A aeno?ao?iiiniiiiio o?ainaai? ?aeiia?iaoe?y a?aeaoeany
ca o/anoth ei?ioeeo AO-aaaaoeo ioeeaioeaeieo iine?aeiaiinoae.

6. Aeno?ao?iiiniiiee o?ainaai caeaoiee noaa?eueii ?aie?eoaaoeny o
ee?oeiao eiiao aac ci?ie eiai no?oeoo?e.

7. Oaiioeiia? a?aei?iiino? o?ainaaiieo ieoae a?ae ii?iaeueieo oaa?ei
coiiaeai? ?ina?oe?eiith ?iaeoeaaoe??th aai ii?ooaiiyi aeni?an?? ie?aieo
aai?a, /e caaaeueiith aeanoaa?e?caoe??th aaiiio ?aoeei??ioa.

IA?AE?E ?IA?O C OAIE AeENA?OAOe??

1. *aueia E.E., Iao?aiei A.E., Aaaeia E.I., E?enai E.A., Nieiiei A.I.
Iiaeeoeeaoeey no?oeoo?u AeIE ieacieaeu pATV8 o o?ainaaiiuo iuoae. 1.
Enneaaeiaaiea no?oeoo?u eioaa?e?iaaiiiai o?ainaaiiia e aeieiae/aneea
yooaeou o?ainaaiica / / Aeiiieeia?u e eeaoea.- 1997.- O.13, ? 4.-
N.323-327.

2. *aueia E.E., E?enai E.A., Iaoeianeee A.E., Nieiiei A.I. Iiaeeoeeaoeey
no?oeoo?u AeIE ieacieaeu pATV-8 o o?ainaaiiuo iuoae. 2. Oece/aneia
ea?oe?iaaiea aaoiiiiiiai o?ainaaiiia e neeaaie?iaaiea eeiie?iaaiiuo
o?aaiaioia aai iiaeeoeoee?iaaiiiai o/anoea / / Aeiiieeia?u e eeaoea.-
1997.- O.13, ? 5.- N.1-6.

3. E?enai E. A., Eeoii E. I., No?ieianeay E. E., Nieiiei A. I.
Iiaeeoeeaoeey no?oeoo?u AeIE ieacieaeu pATV8 o o?ainaaiiuo iuoae. 3.
Aiaeec ioeeaioeaeiie iineaaeiaaoaeueiinoe yeno?ao?iiiniiiiai o?ainaaia e
aai ?aieeeaoeey a eeaoeao ianaeiiuo / / Aeiiieeia?u e eeaoea.- 1999.-
O.15, ? 1.- N.57-62.

AIIIOAOeEss

E?enai E.A. Ia?ano?ieee no?oeoo?u ieacieaeu pATV 8 o o?ainaaiiuo
iuoae.-?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe
ii niaoeeaeueiinoe 03.00.03 — iieaeoey?iay aeieiaey.— Einoeooo
iieaeoey?iie aeieiaee e aaiaoeee IAI Oe?aeiu, Eeaa, 1999.

Aeenna?oaoeey iinayuaia i?iaeaiai acaeiiaeaenoaey o?ainaaia e aaiiia
oicyeia a iiaeaeueiie nenoaia. Iieacaii, /oi i?e aaaaeaiee a ceaiou iuoe
ieacieaeu pATV 8, ninoiyuae ec aaeoa?eaeueiiai aaeoi?a pBR 322 e
i?iae?oniie AeIE ae?ona na?eiiu ?aona, /anoue iieo/aiiuo o?ainaaiiuo
iuoae niaea?aeeo o?ainaai a yeno?ao?iiiniiiie, a /anoue — a
eioaa?e?iaaiiie a aaiii oi?ia. Eae yeno?ao?iiiniiiay, oae e
eioaa?e?iaaiiay oi?iu noaaeeueii ianeaaeiaaeenue iioiieaie o?ainaaiiuo
iuoae.

Ioia/aii, /oi iaa oi?iu o?ainaaia i?aoa?iaee aeoaieea ia?ano?ieee,
eioi?ua i?iecioee ia naiuo ?aiieo noaaeeyo yia?eiiaeueiiai ?acaeoey,
iineieueeo o?ainaaiiua iuoe ia auee iicaeeaie. I?e yoii, eciaieaoenue
iaeiie?aoii, no?oeoo?a o?ainaaiia noaaeeece?iaaeanue, e a iineaaeothueo
iieieaieyo o?ainaaiiuo iuoae ia eciaiyeanue. I?e eioaa?aoeee ec ninoaaa
o?ainaaia yeeieie?iaaeenue ana i?iae?oniua e aieueoay /anoue
aaeoa?eaeueiuo ioeeaioeaeiuo iineaaeiaaoaeueiinoae. Iaeei ec eiioeia
eioaa?e?iaaiiiai o?aaiaioa i?eiaaeeaaeeo aaio onoie/eainoe e
aiieoeeeeeio pBR 322, a aoi?ie — aaio-?ai?anni?o, ia?aie/eaathuaio
eiieeiinoue ieacieaeu a eeaoeao E. coli, ?acia? o?aaiaioa — ieiei 1,5
o.i.i.. ?acia? yeno?ao?iiiniiiiai o?ainaaia — 5,5 o.i.i., io enoiaeiie
ieacieaeu ii ioee/aaony aeaeaoeeae cia/eoaeueiie /anoe i?iae?oniuo e
/anoe aaeoa?eaeueiuo ioeeaioeaeiuo iineaaeiaaoaeueiinoae. Inoaaoeeny
o?aaiaio i?iae?oniie AeIE ?acia?ii ieiei 1,8 o.i.i. niaea?aeeo ii/oe
iieioth iineaaeiaaoaeueiinoue aaia env e iaaeaaiioth iaeanoue aaiia env
e src. Yoio o?aaiaio aue o?ainiice?iaai ec naeoa eeiie?iaaiey
i?iae?oniie AeIE a ae?oaie, aeeceaaeauee o/anoie iieaeoeu. Aeaeaoeey
aaeoa?eaeueiie /anoe o?ainaaia cao?iioea oiiieiaaoeeny ?aiaa
aai-?aaoeyoi? eiieeiinoe. Aiaeec a?aieoe ia?ano?iaiiuo o/anoeia iieacae,
/oi ana iie auee oeaiee?iaaiu ei?ioeeie AO-aiaaouie
iineaaeiaaoaeueiinoyie. Enneaaeiaaiea iniaaiiinoae ioeeaioeaeiie
iineaaeiaaoaeueiinoe o?ainaaia iicaieeei naeaeaoue auaiae i oii, /oi
i?e/eiie aai ia?ano?iae yaeythony ?aeiiaeiaoeey n eeaoi/iuie aiiieiaaie
i?iae?oniuo aaiia e ioeeaaciay aeaa?aaeaoeey, eieoeee?iaaiiay a ianoao
eieaeueiiai ieaaeaiey AeIE o?ainaaia e ia?aciaaiey iaeiioeaii/a/iuo
o/anoeia.

Noaaeeueioth yeno?ao?iiiniiioth ?aieeeaoeeth o?ainaaia iaania/eaaee
iiaiia?aciaaiiua a ?acoeueoaoa aai ia?ano?iae ioeeaioeaeiua
iineaaeiaaoaeueiinoe. Aiaeec yoeo iineaaeiaaoaeueiinoae iicaieee
auyaeoue o/anoee, aiiieiae/iua eaiiie/aneie iineaaeiaaoaeueiinoe ori
?aieeeaoeee yoea?eio.

Onoaiiaeaii, /oi i?e o?ainoaeoeee a eoeueoo?o eeaoie ianaeiiuo,
yeno?ao?iiiniiiue o?ainaai iuoe niiniaai onoie/eai ?aieeoee?iaaoueny e
naa?aae?iaaoue aac eioaa?aoeee a aaiii e eciaiaiey no?oeoo?u.
Eiio?ieueiua ieacieaeu pBR 322 e pATV 8 a aiaeiae/iii yenia?eiaioa ia
?aieeoee?iaaeenue e yeeieie?iaaeenue ec eeaoie iinea 1-2 eeaoi/iuo
aeaeaiee. I?aaeiieaaaaony, /oi ?aieeeaoeey o?ainaaia a eeaoeao ianaeiiuo
iaania/eaaeanue oaie aea iineaaeiaaoaeueiinoyie, /oi e a eeaoeao iuoe,
/oi aiai?eo i aunieie eiina?aaoeaiinoe ?aieeeaoeaiiai aiia?aoa yoea?eio.

Eeth/aaua neiaa: o?ainaaiiua iuoe, i?iae?oniay AeIE ae?ona na?eiiu
?aona, eioaa?aoeey, ia?ano?ieee o?ainaaia, ?aieeeaoeey, o?ainoaeoeey.

AIIOAOe?ss

E?enai E.A. Ia?aaoaeiae no?oeoo?e ieaci?aee pATV 8 o o?ainaaiieo
ieoae.-?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo iaoe
ca niaoe?aeuei?noth 03.00.03 — iieaeoey?ia a?ieia?y.— ?inoeooo
iieaeoey?ii? a?ieia?? ? aaiaoeee IAI Oe?a?ie, Ee?a, 1999.

Aeena?oaoe?th i?enay/aii i?iaeaiai aca?iiae?? o?ainaaio ? aaiiio oacy?ia
o iiaeaeuei?e nenoai?. Iieacaii, ui i?e aaaaeaii? a ceaioe ieo?
ieaci?aee pATV 8, yea i?noeoue i?ia??onio AeIE a??ono na?eiie ?aona,
/anoeia io?eiaieo o?ainaaiieo ieoae i?noeoue o?ainaai a
aeno?ao?iiiniii?e, a /anoeia — a ?ioaa?iaai?e o aaiii oi?i?.
A?aecia/aii, ui iaeaea? oi?ie o?ainaaio caciaee aeeaieeo ia?aaoaeia.
Aiae?c a?aieoeue ia?aaoaeiaaieo ae?eyiie aeicaieea aecia/eoe iaoai?cie
ia?aaoaeia o?ainaaio oa aeacaoe ia iniaeeaino? eiai ioeeaioeaeii?
iine?aeiaiino?, ui i?ecaaee aei oeeo ia?aaoaeia. Anoaiiaeaii, ui
aeno?ao?iiiniiiee o?ainaai ieo? caeaoiee noaa?eueii ?aie?eoaaoeny aac
no?oeoo?ieo ci?i o eoeueoo?? ee?oei eiiao. Aeine?aeaeaiiy eiai
ioeeaioeaeii? iine?aeiaiino? aeicaieeei aeyaeoe ae?eyiee, aiiieia?/i?
eaiii?/i?e iine?aeiaiino? ori ?aie?eaoe?? aoea??io?a.

Eeth/ia? neiaa: i?e?i?i’?eoe?y o ceaioe, o?ainaaii? ieo?, a??on na?eiie
?aona, ?ioaa?aoe?y, ia?aaoaeiae o?ainaaio, ?aie?eaoe?y, o?ainoaeoe?y.

ANNOTATION

Krysan K.V. Rearrangements of plasmid pATV 8 structure in transgenic
mice.-Manuscript.

Thesis for candidate’s of sciences degree on speciality 03.00.03 —
molecular biology.— Institute of Molecular Biology and Genetics,
Ukrainian NAS, Kyiv, 1999.

Dissertation is devoted to the problem of interaction between host
genome and transgene in the model system. It has been shown, that after
introduction of plasmid pATV 8 into mouse zygotes, one part of the
transgenic mice contained extrachromosomal and another part — integrated
form of the transgene. Both of the forms have undergone an essential
rearrangements. Analysis of the boundaries of rearranged regions allowed
us to determine the mechanisms of such rearrangements and the features
of transgene sequence, led to their appearance. It was also found, that
murine extrachromosomal transgene is able to replicate continuously in
Insect cells without structural changes. Investigation of its nucleotide
sequence has revealed the regions of homology to the eukariotic ori
replication consensus sequence.

Key words: microinjection into zygotes, transgenic mice, Rous sarcoma
virus, integration, transgene rearrangements, replication, transfection.

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