Iaoe?iiaeueia Aeaaeai?y iaoe Oe?a?ie

?inoeooo ee?oeiii? a?ieia?? ? aaiaoe/ii? ?iaeaia???

  ?OAOeIAA Ie?ineaaa Ieaenaiae??aia

OAeE
581.143.6:578.21

IAeA?AEAIIss I?AEAEAeIAEO A?A?EAe?A ?

O?AINAAIIEO ?INEEI A?A*EE OA ?O AIAE?C

03.00.15 — aaiaoeea

Aaoi?aoa?ao

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa a?ieia?/ieo iaoe

Ee?a — 1998

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia a ?inoeooo? o?c?ieia?? ?ineei ? aaiaoeee

IAI Oe?a?ie

Iaoeiaee ea??aiee — aeieoi? a?ieia?/ieo iaoe,

Eaaaiei Ai?en Ieaen?eiae/,

?inoeooo o?c?ieia?? ?ineei ? aaiaoeee

IAI Oe?a?ie, caa. a?aeae?eii

Io?oe?ei? iiiiaioe — aeieoi? a?ieia?/ieo iaoe,

Aaee?i Aiaoie?e Iaaeiae/,

?inoeooo a?ii?aai?/ii? o?i?? ?

iaooio?i?? IAI Oe?a?ie, caa.a?aeae?eii

aeieoi? a?ieia?/ieo iaoe,

Ia??e Oaae?? Ieeeoiae/,

?inoeooo oeoe?iaeo ao?ye?a OAAI,

i?ia?aeiee iaoeiaee ni?a?ia?oiee

I?ia?aeia onoaiiaa-Ee?anueeee oi?aa?neoao ?i. Oa?ana Oaa/aiea.

Caoeno a?aeaoaeaoueny «21” n?/iy 1999 ?. i 14 aiae. ia ca-

n?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae.26.202.01 i?e ?inoe-

ooo? ee?oeiii? a?ieia?? ? aaiaoe/ii? ?iaeaia??? IAI Oe?a?ie ca

aae?anith: 252143, Ee?a, aoe. Caaieioiiai, 148.

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? ?inoeoooo

ee?oeiii? a?ieia?? ? aaiaoe/ii? ?iaeaia??? IAI Oe?a?ie , Ee?a,

aoe. Caaieioiiai, 148.

Aaoi?aoa?ao ?ic?neaiee » 20 » a?oaeiy 1998 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee E.A.Oa?anaiei

 CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue i?iaeaie .A?a/ea ? iaei??th c iaeaaaeeea?oeo
e?oi’yieo eoeueoo?. Ia?iaeiiainiiaea?nueea cia/aiiy ??
aecia/a?oueny aenieeie iiaeeaieie ? ae??oe/ieie yeinoyie a?a/aii?
i?iaeoeoe??, yea i?noeoue aeia?a canaithaai? a?eee ye? neeaaeathoueny
a iniiaiiio ?c aeiaoe?io ? aethoai?io. A?ieia?/ia oe?ii?noue a?ee?a
a?a/ee — iaeaeua, o ii??aiyii? c ?ioeie eoeueoo?aie. E?oia i?noeoue
no?ee? aei ieeneaiiy aee?e; aeoaea ei?eni? aeey i?aai?cio ethaeeie
i?aai?/i? eeneioe (eeiiiia, yaeo/ia, uaaeaaa). Aiia aaaaoa a?oai?iaie
(Naaeoeueeee, 1970;  E?ioia, 1975).

A?a/ea — aeia?ee iaaeiiin ? ni?ey? ?icaeoeo aaeae?eueieoeoaa. ?? /anoi
canoiniaothoue, ye no?aoiao ? e?ea?nueeo ?ineeio.

I?ioa ieiu? ?? ae?iuoaaiiy ia Oe?a?i? c eiaeiei ?ieii
ciaioothoueny, oiio ui a?a/ea oa?aeoa?eco?oueny niaoeeo?/ieie
iniaeeainoyie a?ieia?? aeeaeaiiy, ?icaeoeo ? oea?o?iiy, ui noaaeoue ??
a ?yae eoeueoo?, i?eioeeaeo aei oiia ae?iuoaaiiy. Aiia aeiiaeeaa aei
oaiia?aoo? ? aieiae (Aeaenaaaa, Iaooaaa,1988). ?aeiiiaai? ni?oe
a?a/ee, iath/e aenieee aaiaoe/iee iioaioe?ae, oa?aeoa?ecothoueny
ianoaa?euei?noth a?iaeaeiino?.

No/ani? ?? ni?oe iio?aaothoue iie?ioaiiy ?yaeo oaeeo iciae, ye
caeaoi?noue aei naiicaieeaiiy, no?ee?noue aei no?aniaeo /eiiee?a
na?aaeiaeua, no?ee?noue i?ioe oai?ia ? oe?aeiee?a. Oe? iciaee iathoue
aeee? aeaee a?a/ee, cie?aia oaoa?nueea. Oiio caeo/aiiy ?o ye aeiii??a
aaaeaieo iciae aeey iie?auaiiy i?e?iaee ?ineei a?a/ee ? aaaeeeaei
ieoaiiyi o naeaeoe?? oe??? eoeueoo?e.

I?iaaaeai? ?iaioe ii no?auoaaiith eoeueoo?ieo ? aeeeeo aeae?a a?a/ee
ia aeaee i/?eoaaieo ?acoeueoao?a, ine?eueee ?niothoue aa?’??e
i?aeaeaeiai? ianoi?niino? (Morris, 1952; A?eoeia,1976; Ruszkowski,
1980; Adachi et al., 1989; Samimy, 1991).

Ae??oaiiy i?iaeaie ianoi?niino? o a?a/ee iio?aao? canoinoaaiiy
iiaeo i?aeoiae?a, ii?aeiaiiy iao?aaeeoe?eieo iaoiae?a, oaeeo ye
eoeueoo?a ee?oei ? i?aai?a c o?aaeeoe?eii canoiniaaiith a naeaeoe??
a?aeaeaeaiith a?a?eaeecaoe??th.

Canoinoaaiiy i?eioniaiai caieeaiiy i?ae ianoi?nieie oi?iaie oa
noeioethaaiiy o?c?ieia?/ii aeoeaieie ?a/iaeiaie i?i?inoaiiy ieeeo ?
?inoo ieeeiaeo o?oaie o aeae?a ye? ia no?auothoueny, aeicaieythoue
iiaeieaoe ia?oee aa?’?? i?aeaeaeiai? ianoi?niino?.

I?ney aeei?enoaiiy iaoiaeo eoeueoo?e oeaieie iiai?noth /e /anoeiai
aeieathoueny ua aeaa aa?’??e, ye? ?niothoue i?e a?aeaeaeai?e
a?a?eaeecaoe??: iaaeeoo?caeaoi?noue a?a?eaeieo ca?iaee?a ?
noa?eeuei?noue a?a?eae?a ia?oiai iieie?iiy.

Aeey noai?aiiy oe?iieo oi?i a?a/ee, ye? aoaeooue iin?yie iio??aieo
iciae, iaiao?aeii c iaeiiai aieo, caeo/aoe i?e?iaei? aeiii?e aai?a
(aeee? aeaee), a c ae?oaiai — iaea?aeoaaoe ?ineeie aeueoa?iaoeaieie
iaoiaeaie.

A no/aniiio n?euenueeiainiiaea?nueeiio ae?iaieoeoa? aa?a?oeeaee
a?ae?a?athoue aaaeeeao ?ieue. Aiie eiio?ieththoue /enaeuei?noue
ao?’yi?a, /ei ni?eythoue caa?aaeaiith a?iaeath. Iiaa iieie?iiy
aa?a?oeeae?a ii?aeio? aenieo aoaeoeai?noue c a?aenooi?noth oiene/iino?
aeey ethaeeie oa oaa?ei ? oa?aeoa?eco?oueny oaeaeeith aeaa?aaeaoe??th
o a?oio? (De Block et al., 1987). I?ioa, oe? aa?a?oeeaee ia ?
naeaeoeaieie ? i?eai?/othoue ??no oa ?icaeoie an?o ?ineei. Iaea?aeaiiy
aa?a?oeeaeino?eeeo ?ineei aea? iiaeeea?noue canoiniaoaaoe ?o ia
i?ioyc? anueiai aaaaoaoe?eiiai ia??iaeo (Piruzian et al.,1988).

Aa?a?oeeae oino?iio?eoeei (AANOA) a?aeiineoueny aei aa?a?oeeae?a iiaiai
iieie?iiy. A?aeiiino? i?i io?eiaiiy o?ainaaiieo ?ineei a?a/ee, no?eeeo
aei oino?iio?eoeeio oa ?ioeo aa?a?oeeae?a, a e?oa?aoo?? a?aenooi?.

?iaioa o oeeo iai?yiao aeeeeea? ye i?aeoe/iee, oae ? oai?aoe/iee
?ioa?an.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie.

Aeena?oaoe?eia ?iaioa aeeiioaaeanue o a?aeiia?aeiino? c oaiaoe/iei
ieaiii a?aeae?eo aaiaoe/ii? ?iaeaia??? ?O?A IAI Oe?a?ie ia 1994 — 1997
??. (oaia «Aea/aiiy aeni?an?? ? aca?iiae?? aai?a no?eeino? aei
aa?a?oeeae?a ? eiiao o o?ainaaiieo ?ineei»,2.34.5.2) oa i?iaeoii AeEIO
ia 1992-1996 ??. (oaia «?ic?iaea iaoiae?a iaea?aeaiiy a?aeaeaeaieo
a?a?eae?a a?a/ee», N3.01.15/138).

Iaoa ? caaea/? aeine?aeaeaiiy .  Iaoith ?iaioe aoea ?ic?iaea iaoiaeo
iaea?aeaiiy i?aeaeaeiaeo a?a?eae?a a?a/ee i?ae Fagopyrum  esculentum
 M. i  Fagopyrum tataricum  L. c aeei?enoaiiyi niiniao aei?iuoaaiiy
a?a?eaeieo ca?iaee?a a oiiaao  in vitro  oa ?aaaia?aoe?? ?ineei ?c
eaeonieo oeaiei, io?eiaieo ?c a?a?eaeieo ca?iaee?a. Iaea?aeaiiy
o?ainaaiieo ?ineei a?a/ee, no?eeeo aei aa?a?oeeaeo oino?iio?eoeeio.

Iniiai? caaea/? aeine?aeaeaiiy:

1.Iiaeieaoe iinoaaiio ianoi?ni?noue a aeine?aeieo i?aeaeaeiaeo
eiia?iaoe?yo no?auoaaiue a?a/ee, canoiniaoth/e aiaeiee ?ic/ei
oei?enoiai eaaei?th.

2.I?ae?a?aoe neeaae iiaeeaiiai na?aaeiaeua aeey aoaeoeaiiai
aei?iuoaaiiy a?a?eaeieo ca?iaee?a aei c??eeo ?ineei.

3.Aecia/eoe iioeiaeueiee a?e a?a?eaeieo ca?iaee?a, iaiao?aeiee aeey
iaeneiaeueiiai ?o aeaeeaaiiy a oiiaao in vitro.

4.?ic?iaeoe iaoiaeeeo aoaeoeaii? ?aaaia?aoe?? ?ineei ?c eaeonieo
oeaiei a?a?eaeieo ca?iaee?a oa io?eiaoe ?ineeie — ?aaaia?aioe.

5.I?aeoaa?aeeoe a?a?eaeio i?e?iaeo iaea?aeaieo ?ineei c aeiiiiiaith
iaoiaeo ?cioa?iaioiiai aiae?co oa i?iaanoe oeeoiaaiaoe/iee aiae?c
a?a?eae?a.

6.?ic?iaeoe iaoiaeeeo io?eiaiiy o?ainaaiieo ?ineei a?a/ee, no?eeeo
aei aa?a?oeeaeo oino?iio?eoeeio, oeyoii i?yii? ?aaaia?aoe?? ?c
aenieaio?a.

7.I?aeoaa?aeeoe ?ioaa?aoe?th bar aaia a aaiii iaea?aeaieo ?ineei
iieaeoey?ii -a?ieia?/iei aiae?cii.

8.Aecia/eoe ??aaiue oaiioei?/ii? aeni?an?? bar aaia o o?ainaaiieo
?ineei.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a .  Aia?oa ?ic?iaeaiee iaoiae
iiaoaiiiai iiaeieaiiy aa?’???a i?aeaeaeiai? ianoi?niino? o a?a/ee:
i?iaaiii? ianoi?niino?, caaeaae? a?a?eaeieo ca?iaee?a ? noa?eeueiino?
a?a?eae?a ia?oiai iieie?iiy.

Aia?oa io?eiaii i?aeaeaeia? a?a?eaee i?ae a?a/eith cae/aeiith
(Fagopyrum esculentum M.), oa aeaiia i?aeaeaeaie (F. tataricum  L.v.
rotundatum  ? F.tataricum L. v. tuberculatum ) oaoa?nueei? a?a/ee.

Aia?oa i?iaaaeaii ii??aiyiiy aoaeoeaiino? aeaio iaoiae?a iaea?aeaiiy
a?a?eae?a: aei?iuoaaiiy a?a?eaeieo ca?iaee?a a oiiaao  in vitro oa
?aaaia?aoe?? ?ineei ?c eaeonieo oeaiei a?a?eaeieo ca?iaee?a. C’yniaaii,
ui io?eiaiiy a?a?eae?a /a?ac eaeonio eoeueoo?o ca?iaee?a a -2-3 ?ace
aoaeoeai?oa, i?ae ?o aei?iuoaaiiy. Iieacaii, ui iaea?aeai? iaoiaeii
?aaaia?aoe?? ?c eaeono a?a?eaeieo ca?iaee?a ?ineeie, ? a a?eueoino?
aio?aeeiei?aeieie, oiae? ye a?a?eaee, io?eiai? i?e aei?iuoaaii?
ca?iaee?a — aio?aaiei?aeieie.

Anoaiiaeaii, ui oa?iaioe aeeiaieueaeaa?ae?iaaiaca e
6-oinoiaethei-iaoaeaa?ae?iaaiaca iiaeooue neoaoaaoe ia?ea?ieie aeey
a?aeai?o ?noeiieo a?a?eaeieo ?ineei a?a/ee.

Aia?oa oeyoii i?yii? ?aaaia?aoe?? ?c aenieaio?a ?ic?iaeaia iaoiaeeea
o?ainoi?iaoe?? cae/aeii? oa i?aeaeaeiaeo a?a?eae?a a?a/ee.

Aia?oa iaea?aeai? o?ainaaii? ?ineeie a?a/ee c ainiiaea?nueei —
oe?iiith iciaeith no?eeino? aei aa?a?oeeaeo oino?iio?eoeeio.

  I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a .

1.?ic?iaeaia noaia iiaoaiiiai iiaeieaiiy aa?’???a i?aeaeaeiai?
ianoi?niino? o a?a/ee ?aeiiaiaeiaaia ye aoaeoeaiee can?a iaea?aeaiiy
i?aeaeaeiaeo a?a?eae?a c oe?iieie iciaeaie.

2.Io?eiai? i?aeaeaeia? a?a?eaee iiaeooue aooe aeei?enoai? a
naeaeoe?ei?e i?aeoeoe?.

3.Cai?iiiiiaaia iaoiaeeea iaea?aeaiiy o?ainaaiieo ?ineei a?a/ee
aeicaieeoue io?eioaaoe oe?ii? aeey naeaeoe?? ni?oe, oeyoii
?io?iaeoeoe?? aai?a, ye? eiaeothoue aaaeeea? iciaee.

4.Io?eiai? o?ainaaii? ?ineeie a?a/ee, no?ee? aei aa?a?oeeaeo
oino?iio?eoeeio, iiaeooue aooe canoiniaai? a n?euenueeiainiiaea?nueeiio
ae?iaieoeoa?.

Iniaenoee aianie caeiaoaa/a. Aeena?oaio ii?aoethaaea iaoeiao e?oa?aoo?o
oa iaieiae?ea iaiao?aeieie iaoiaeaie aeine?aeaeaiue, a?aea o/anoue a
i?aeaioiaoe? oa i?iaaaeaii? aenia?eiaio?a, aiae?c? oa ?ioa?i?aoaoe??
iaea?aeaieo ?acoeueoao?a. An? iniiai? aenia?eiaioaeuei? aeine?aeaeaiiy
oa ?o ia?iaea aeeiiai? aeena?oaioii.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??. ?acoeueoaoe aeine?aeaeaiue
aeena?oaoe?eii? ?iaioe aeiiia?aeaeenue ia eiioa?aioe?? iieiaeeo a/aieo
» Aeooaeuei? i?iaeaie o?c?ieia?? ?ineei ? aaiaoeee» (Ee?a,1992); ia VI
c’?cae? Oe?a?inueeiai oiaa?enoaa aaiaoee?a ? naeaeoe?iia??a ?i.
Aaa?eiaa (Iieoaaa, 1992); ia I?aeia?iaeiiio neiiic?oi? «A?ioaoiieia?y oa
aaiaoe/ia ?iaeaia??y ?ineei» (Ee?a, 1994); VI eiioa?aioe?? iieiaeeo
a/aieo, i?enay/ai?e 50-e ??/ieoe? c aeiy canioaaiiy ?O?A IAI Oe?a?ie
(Ee?a,1996); ia VII I?aeia?iaei?e eiioa?aioe?? » A?ieia?y ee?oei ?ineei
 in vitro, a?ioaoiieia?y ? caa?aaeaiiy aaiioiiaeo (Iineaa, 1997);
I?aeia?iaei?e eiioa?aioe?? «Aa?ia?ioaoiieia?y ?ineei ? oaa?ei»
(Ee?a,1997); ia ?? I?aeia?iaeiiio neiiic?oi? ii a?ioaoiieia?? ?ineei
(Ee?a, 1998).

Ioae?eaoe??. Ca ?acoeueoaoaie aeena?oaoe?? iioae?eiaaii 12 iaoeiaeo
?ia?o, a oiio /ene? 3 noaoo? o i?ia?aeieo aeaeaiiyo oa iiaeaia
cayaea ia aeiao?ae ? 98074048, Cayae. 23.07.1998; Iioae.

No?oeoo?a oa ianya aeena?oaoe??. Aeena?oaoe?y aeeeaaeaia ia 132
noi??ieao iaoeiiieniiai oaenoo. Neeaaea?oueny ?c nieneo oiiaieo
nei?i/aiue, anooio, iaeyaeo e?oa?aoo?e, aenia?eiaioaeueii? /anoeie c
iaaiai?aiiyi iaea?aeaieo ?acoeueoao?a, aeniiae?a ? nieneo aeei?enoaieo
aeaea?ae c 250 iaeiaioaaiue, a oiio /ene? 159 ia ?iicaiieo iiaao.
I?noeoue 12 oaaeeoeue ? 20 iaethie?a.

IAOA??AEE OA IAOIAeE AeINE?AeAEAIUe

Iaoa??aeii aeine?aeaeaiue aoee — a?a/ea eoeueoo?ia — Fagopyrum
esculentum M. (2n=16) , yea i?aaenoaaeaia ni?oii Aeno?a oa
naiinoiinieie oi?iaie: Caeaiieaioeiaa, Aiiinoeeieia oa Aeiii?
Aiei?onueeee — naeaeoei? Iinoeoooo caiea?ianoaa OAAI, oa aeeeee aeae
oaoa?nueei? a?a/ee —  Fagopyrum tataricum  L. (2n=16) aeaio
i?aeaeae?a:  rotundatum  — (aeae? — E-17), ?  tuberculatum  — (aeae? —
E-21). Oeae aeae iaea?aeaiee c eieaeoei? AI?o.

Ooo/ia caieeaiiy ea?o?a eoeueoo?ii? a?a/ee i?iaiaeeee ieeeii
oaoa?nueei?. Oa?oeeueiinoue ieeeiaeo ca?ai aecia/aee eiaeiei iaoiaeii,
a ?o aeeoo?caeaoi?noue ca iaoiaeeeith Aeaen??ai?, Iaooaai? (1988).

Aeey iiaeieaiiy iinoaaiii? ianoi?niino? canoiniaoaaee 0,05; 0,1; 0,15%
-i? aiaei? ?ic/eie nieae oei?enoiai eaaeiith ca
?aeiiaiaeaoe??th Iaeueie/oea TH.I. (1990). Aeineiaeaeoaaee ?o /aniao
aeith ia i?eeii/ee iaoi/ie eoeueoo?ii? a?a/ee, ia ye? no?oooaaee a
iaeieo aeiaaeeao nooee ieeie aeeeiai aeaeo, o ae?oaeo — caii/aiee ia
3-5 oa. o ?ic/ei? CdCl2 a eiioeaio?aoe??, aiaeia?/i?e o?e, ui
iiia?aaeiuei iaiineee ia i?eeii/eo. O eiio?ieueieo eiia?iaoe?yo
no?auoaaiue NdCl2 ia canoiniaoaaee.

Ca i?i?inoaiiyi ieeeiaeo o?oaie o noiai/eee iaoi/ie niinoa??aaee
ca iaoiaeeeith Aeaen??ai?, Iaooaai? (1988).

Aeey aaaaeaiiy a?a?eaeieo ca?iaee?a a eoeueoo?o in vitro, 6-20-oe
aeaii? ieiaee noa?ee?coaaee o 96%-iiio nie?oi 1 oa. oa a 5%-iiio ?ic/ei?
oei?aiiio — 15 oa. Ca?iaeee aeae?eyee ? aei?iuoaaee ia ca?aaeiaeu? IN
(Murashige & Skoog, 1962), yea aeiiiaithaaee o?oiai?iiiaie (AAI, ?IE,
?IE, IIE) a ??cieo eiioeaio?aoe?yo ? ni?aa?aeiioaiiyo.

Aeey ?iaeoeoe?? eaeoniooai?aiiy ? ianooiii? ?aaaia?aoe?? ?ineei ?c
eaeono a?a?eaeieo ca?iaee?a a?a/ee canoiniaoaaee na?aaeiaeiaeua IN
aeiiiaiaia AAI, ?IE, IIE. ?eciaaiac o iaea?aeaieo iaaii?a noeioethaaee
ia na?aaeiaeu? IN a i?enooiino? IIE oa ?IE.

A?a?eaei? ?ineeie ae?iuoaaee ia na?aaeiaeu? IN c aeii?oeaie IIE oa AAI,
i?e 26± 20N oa 16-aiaeeiiiio oioiia??iae?.

Inoeiiinoue aia?eaeia, iaea?aeaieo i?ney no?auoaaiiy eoeueoo?iiai c
aeeeei aeaeii oaoa?nueei? a?a/ee, iiaeoaa?aeaeoaaee, canoiniaoth/e
iaoiae icioa?iaioiiai aiaeico (Aeacei, Niceiia, 1993).

Oeeoiaaiaoe/iee aiae?c io?eiaieo a?a?eae?a i?iaiaeeee a ee?oeiao
ei?aiaai? ia?enoaie ?ineei, eoeueoeaiaaieo in vitro, ca noaiaea?oiith
iaoiaeeeith aeaaeaieo i?aia?ao?a (Iaooaaa, 1980). O eiaei?e ?ineei?
aiae?coaaee ia iaioa 20-30 iaoaoac. Aeine?aeaeaiiy i?aia?ao?a
cae?enithaaee ia i?e?ineii? «Amplival» i?e ca?eueoaii? 100o.
I?e?ioioia?aooaaiiy aeeiioaaee ia ie?aoe? «I?e?ao» 300.

sse aeo?aeiee iaoa??ae aeey iaea?aeaiiy no?eeeo aei aa?a?oeeaeo
oino?iio?eoeeio ?ineeie a?a/ee aeei?enoiaoaaee oi?ie c ??ciith
?aaaia?aoe?eiith caeaoi?noth: Aiiinoee?eio oa a?a?eaee Aeno?a o E-17,
Caeaiiea?oeiaa o E-17 ? Aiiinoee?eia o E -17. Aenieaioe (eenoee,
/a?aoee, i?aeaocey) oeeo ?ineei ia?iaeyee ooaiii Agrobacterium
tumefaciens pGV3850::pBin19/pBA 3 c bar ?  npt  II aaiaie (Cayoe e ae?,
1994).

Aeey ?iaeoeoe?? i?yii? ?aaaia?aoe?? ?ineei aenieaioe eoeueoeaoaaee ia
iiaeeaiiio na?aaeiaeu? IN c aeii?oeaie ai?iii?a (AAI, ?IE, IIE) a
??cieo eiioeaio?aoe?yo ? ni?aa?aeiioaiiyo.

sse naeaeoeai? oaeoi?e aeey a?aeai?o o?ainaaiieo ?ineei canoiniaoaaee
na?aaeiaeua IN c eaiai?oeeiii (100ia/e) oa oino?iio?eoee-iii (30iee/e).
Iieaeoey?ii — aiieiai/iee aiae?c i?aia?ao?a AeIE o?ain-aaiieo ?ineei
cae?enithaaee iaoiaeii aeio-a?a?eaeecaoe?? (Southern, 1975).

Oaiioei?/io aeni?an?th aaio bar o o?ainaaiieo ?ineei a?a/ee ia?oiai
iieie?iiy aecia/aee i?ney ia?iaee eenoy eiia?oe?eiith eiioeaio?aoe??th
(3 — 5 ie/e) aa?a?oeeaeo.

Noaoenoe/io ia?iaeo aenia?eiaioaeueieo aeaieo i?iaiaeeee ca
noaiaea?oiith iaoiaeeeith (Aeiniaoia, 1985).

?ACOEUeOAOE AeINE?AeAEAIUe OA ?O IAAIAI?AIIss

Iiaeieaiiy ia?oiai aa?’??o i?aeaeaeiai? ianoi?niino? o no?auoaaieo
aeae?a a?a/ee . Ine?eueee o eiia?iaoe?yo no?auoaaiue
 Fagopyrum esculentum  x  F. tataricum aeyaeaia iiaia i?iaaiia
ianoi?ni?noue (?oiyioeaaa e ae?., 1987), oi iaiao?aeiith oiiaith
iaea?aeaiiy i?aeaeaeiaeo a?a?eae?a aoei i?iaaaeaiiy ooo/iiai caieeaiiy
o ii?aeiaii? ?c aeei?enoaiiyi o?c?ieia?/ii aeoeaii? niieoee —
oei?enoiai eaaei?th (Iaeueie/oe, 1990).

Aiae?c ?acoeueoao?a, io?eiaieo o oiae? iieueiaeo aeine?aeaeaiue,
iieacaa, ui aeoeaia i?i?inoaiiy ieeeo aeeeiai aeaeo ? ??no ieeeiaeo
o?oaie o noiai/eeao iaoi/ie eoeueoo?ii? a?a/ee a?aeaoaaeeny iiney
30-oaeeeiii? ae?? ia i?eeii/ee 0,1%-iiai ?ic/eio oei?enoiai
eaaeiith, ui i?eaaei aei i?aeaeuaiiy a?aenioea ooai?aiiy caa’ycae o an?o
eiia?iaoe?yo no?auoaaiue a ii?iaiyiii c eiio?ieai oa ?ioeie
eiioeaio?aoe?yie CdCl2 (oaae.1).

C’ynoaaee, ui i?ney canoinoaaiiy caii/aiiai ia 3-5 oa. a 0,1%
aiaeiiio ?ic/ei? oei?enoiai eaaei?th ieeeo a?aenioie ooai?aiiy caa’ycae
aoa aeuei, i?ae i?ney no?oooaaiiy eiai nooei o an?o aeine?aeaeoaaieo
eiia?iaoe?yo no?auoaaiue eoeueoo?ii? a?a/ee c i?aeaeaeii oaoa?nueei?
— E-17. O eiia?iaoe?? Aeno?a o E-21 (?ioee i?aeaeae) ??cieoey a
ooai?aii? caa’ycae, ye i?e nooiio, oae ? i?e caii/aiiio ieeeo aoea
iacia/ia.

Oaeei /eiii, caaaeyee aeei?enoaiith 0,1%-iiai ?ic/eio nieae oei?enoiai
eaaei?th iai aaeaeinue i?aeaeueoe a?aenioie ooai?aiiy caa’ycae o
aeine?aeieo eiia?iaoe?yo no?auoaaiue, a na?aaeiueiio aei 55,7%. O
ii??aiyii? c ?acoeueoaoaie io?eiaieie ?oiyioeaaith (1987),
Oa?aiaiei (1988) ? Nai?i? (1991) i?ney canoiniaoaaiiy o?c?ieia?/ii
aeoeaieo ?a/iaei: aeeiaoeenoeueoieneaeo, oei?ii?oeaoeio, e?iaoeio /e
aac ieo aeey aiaeia?/ieo eiia?iaoe?e no?auoaaiue a?aenioie ca’ycoaaiiy
ia ia?aaeuoaaa 32,1%.

Oaaeeoey 1. Ooai?aiiy caa’ycae o i?aeaeaeiaeo eiia?iaoe?e no?auoaaiue
a?a/ee i?ney canoinoaaiiy aiaeiiai ?ic/eio oei?enoiai eaaei?th

Eiiaiiaoei? iiaeaeaeiaeo no?auoaaiueCanoinoaaiiy ?ic/eio oei?enoiai
eaaei?th Aoaeoeai?noue ooai?aiiy caa’ycae , % ;

eiioeaio?aoe?? CdCl2 :0,05%  0,1%0,15%Aeno.o E-17eiio?ieue 4,9±0,6
4,8±0,3 3,5±0,3Aeno.o E-17noo.ieeie22,3±4,0**35,2±3,3**Aeno.o
E-17caii/.ieeie41,4±3,9**62,4±3,9**38,5±5,1**Aeno.o E-21eiio?ieue
5,4±0,8 4,4±0,1 2,2±0,4Aeno.o E-21noo.ieeie26,8±2,6*52,0±2,6*Aeno.o
E-21caii/.ieeie28,2±4,3**57,3±3,1*25,3±4,0**A.no.o E-17eiio?ieue
3,9±0,7 4,1±0,7 2,8±0,1A.no.o E-17noo.ieeie10,4±3,4**38,0±3,2*A.no.o
E-17caii/.ieeie40,6±3,0**65,5±3,9**34,8±3,1*Aeii.A.o E-17eiio?ieue
5,2±0,1 6,2±0,9 3,4±0,2Aeii.A.o
E-17noo.ieeie10,9±2,6*11,7±2,6*Aeii.A.o
E-17caii/.ieeie38,1±3,9**52,8±4,1**22,4±3,0*Cae.o E-17eiio?ieue 5,1±0,3
4,8±0,2 2,9±0,6Cae.o E-17noo.ieeie11,1±3,5**15,9±3,5**Cae.o
E-17caii/.ieeie38,8±2,9*57,6±7,3**39,9±4,9**

I?ei?oea:* -??cieoey aeinoia??ia i?e ?=0,01; ** -i?e ?=0,001 (ii
a?aeiioaiith aei eiio?ieth); eiio?ieue — caieeaiiy aac canoinoaaiiy
CdCl2 ; noo.ieeie — iaianaiiy nooiai ieeeo ia caieiaeai? aiaeiei
?ic/eiii oei?enoiai eaaei?th i?eeii/ee; caii/.ieeie — iaianaiiy
caii/aiiai a ?ic/ei? CdCl2 ieeeo ia caieiaeai?, aiaeia?/iei ?ic/eiii,
i?eeii/ee iaoi/ie.

Aeno.- ni?o Aeno?a, A.no.- Aiiinoee?eia, Aeii.A. — Aeiii? A?ei?onueeee,
Cae. — Caeaiiea?oeiaa oi?ie, i?aeaeaee oaoa?nueei? a?a/ee- E-17 —
rotundatum, E-21 — tuberculatum.

I?aeaeuaiiy aoaeoeaiino? a?aeaeaeaii? a?a?eaeecaoe?? i?ney
canoinoaaiiy iaoiaeo eoeueoo?e oeaiei. Ianooiiith i?iaeaiith i?e
a?aeaeaeai?e a?a?eaeecaoe?? ?ineei a?a/ee ? iaaenieee a?aenioie
aeaeeaaiiy a?a?eaeieo ca?iaee?a. Aiie aeiooue ia na?aaei?o ?
i?ci?o noaae?yo aia??iaaiaco. *anoi oea ? ?acoeueoaoii a?aenooiino?
aiaeinia?io, /e aiiiaeueiiai eiai ?icaeoeo, aai aeaa?aaeaoe??
aeae?iiiaiai oa?o ee?oei, ui ia?aoeiaeaea? i?iieeiaiith iiaeeaieo
?a/iaei a?ae aiaeinia?io aei ca?iaeea (Ohnishi, 1988).

Aeei?enoaiiy iaoiaeo eoeueoo?e oeaiei aeicaiey? a cia/i?e i???
iiaeieaoe oeth aaaeo, i?ney aeae?eaiiy a?a?eaeieo ca?iaee?a oa
aei?iuoaaiiy ?o ia iiaeeaiiio na?aaeiaeu? c iaiao?aeieie aeaiaioaie
aeeaeaiiy.

Ie canoinoaaee ? ii??aiyee aeaa iaoiaee aeey iaea?aeaiiy
i?aeaeaeiaeo a?a?eae?a: 1) niin?a aei?iuoaaiiy a?a?eaeieo ca?iaee?a aei
c??eeo ?ineei a anaioe/ieo oiiaao; 2) niin?a ?aaaia?aoe?? ?ineei ?c
eaeonieo ee?oei, io?eiaieo ?c aeae?eaieo a?a?eaeieo ca?iaee?a.

Aeey aaaaoueio eoeueoo? a?aeiii, ui iaea?eueo nooo?aee aieea ia
i?ioean ?icaeoeo ?ineei ?c a?a?eaeieo ca?iaee?a iathoue o?oiai?iiie :
oeeoie?i?ie (Ammirato, 1983) aai aoeneie o ii?aeiaii? c oeeoie?i?iaie
(Bhansal et al., 1991; Oioeiaa, O?naiei, 1994).

O ca’yceo c a?aenooi?noth e?oa?aoo?ieo aeaieo a?aeiinii ae??
o?oiai?iii?a ia i?ioean oi?ioaaiiy ?c a?a?eaeieo ca?iaee?a a?a/ee
?ioaeoieo ?ineei ie aea/eee ae?th aoenei?a oa oeeoie?i?i?a ye ie?aii oae
? ?acii ? a ??cieo eiioeaio?aoe?yo.

I?ney aeine?aeaeaiiy 84 aa??aio?a iiaeeaieo na?aaeiaeu anoaiiaeee,
ui oi?ioaaiiy ?ineei ?c a?a?eaeieo ca?iaee?a a?aeaoaaeiny ia
na?aaeiaeu? IN aeiiiaiaiiio o?oiai?iiiaie AAI oa ?IE. Ia ?ioeo
aa??aioao ca?iaeee ia i?i?inoaee aai ?o ??no ? ?icaeoie aoa aeoaea
iia?eueiei.

Iaeaeoeai?oa i?i?inoaiiy ca?iaee?a ia i?ioyc? aeaio oeaei?a a?aecia/aee
ia iiaeeaiiio na?aaeiaeu? IN c aeii?oeaie 0,5 ia/e AAI oa 0,1 ia/e ?IE
aeey an?o aeine?aeaeoaaieo eiia?iaoe?e no?auoaaiue.

Aecia/eee, ui ia i?ioean oi?ioaaiiy  ?ineei  ?c a?a?eaeieo ca?iaee?a
a?a/ee aieeaathoue iaai? ni?aa?aeiioaiiy a iiaeeaiiio na?aaeiaeu?
eiioeaio?aoe?e aoeneio ( ?IE) oa oeeoie?i?io (AAI). Iiae?ai?
?acoeueoaoe aoee iaea?aeai? i?ney aei?iuoaaiiy iaa?a?eaeieo ca?iaee?a
a?a/ee (?oiyioeaaa e ae?., 1988).

Anoaiiaeee, ui /anoioa ooai?aiiy ?ioaeoieo ?ineei caeaaeeoue a?ae a?eo
ca?iaee?a (oaae.2).

Oaaeeoey 2. Caeaaei?noue /anoioe ooai?aiiy ?ineei a?ae a?eo  ca?iaee?a

?

A?e ca?iaee?a

E?euee?noue, oo

*anoioa ooai?aiiy i/i(aei?)aenaaeaeaieo ca?iaee?anoi?iiaaieo
?ineei?ineei, %1 6 — 8570142,5 ±0,628 — 10567264,6 ± 0,9310 —
125649416,7 ± 1,8412 — 1457238667,4 ± 1,4514 — 1657123240,6 ± 1,7616 —
1857012421,8 ± 0,8718 — 20555193,4 ± 0,5

Iniiaia e?euee?noue 6 — 10 — aeaiieo ca?iaee?a, ye? ia i?i?inee
ao??ea oa caneoaea /e iie?eaaeanue eaeoniith oeaieiith. Iaeaieueo
aeeoo?caeaoieie a anaioe/ieo oiiaao aeyaeeenue a?a?eaei? ca?iaeee,
aeaeieaii ia 12 — 14-oee oa 14-16-oee aeaiue iiney caieeaiiy aeey an?o
aeine?aeaeoaaieo eiia?iaoe?e no?auoaaiue. *anoioa ooai?aiiy ?ineei
noaiiaeea 67,4 % oa 40,6% a?aeiia?aeii. 16-20-aeaii? ca?iaeee
i?i?inoaee, ooai?thth/e neeueii ?aaeoeiaaiee iioiauaiee a?iieioeeue ?
aaeee? n?i’yaeieuei? eenoee, ia yeeo ooai?thaaany eaeon.

Ioaea, aecia/eee iioeiaeueiee neeaae iiaeeaiiai na?aaeiaeua oa a?e
ca?iaee?a iaiao?aei? aeey aoaeoeaiiai ?o aei?iuoaaiiy a oiiaao  in
vitro.

Ca aeiiiiiaith aeaiiai iaoiaeo aoei iaea?aeaii 618 ?ineei, ui
noaiiaeoue 50,4% a?ae e?eueeino? aenaaeaeaieo ca?iaee?a.

Iaie aia?oa ?ic?iaeaia iaoiaeeea aoaeoeaii? ?aaaia?aoe?? ?ineei
a?a/ee c eaeono, io?eiaiiai ?c a?a?eaeieo ca?iaee?a. Anoaiiaeaii, ui
iioeiaeueiei aeey iaea?aeaiiy oioeiioaioiiai eaeono ? na?aaeiaeua IN c
aeii?oeaie o?oiai?iii?a AAI, ?IE, IIE. Aiii aeyaeeinue aoaeoeaiei aeey
an?o aeine?aeaeoaaieo eiia?iaoe?e no?auoaaiue.

O a?a?eaeieo ca?iaee?a ia aeaiiio na?aaeiaeu? a?aecia/aee oi?ioaaiiy
ii?oiaaiiiai eaeono. ?aaaia?aoe?y ?ineei (4-6 ianaae) ?c oeueiai eaeono
i?ioiaeeea oeyoii i?aaiiaaiaco c ooai?aiiyi a?oiueie, a iio?i iaaii?a,
ye? caa??aaee ca’ycie c noaeeiieie oeaieiaie eaeono. Aecia/eee, ui aeey
iiaeaeueoiai aei?iuoaaiiy iaea?aeaieo iaaii?a iaiao?aeiith oiiaith ? ?o
ia?aianaiiy ia na?aaeiaeua IN c aeii?oeaie IIE ? ?IE, aeey ?iaeoeoe??
?eciaaiaco.

Aea/aee ?aaaia?aoe?eio caeaoi?noue ii?oiaaiiiai eaeono, iaea?aeaiiai ?c
a?a?eaeieo ca?iaee?a (oaae.3).

 Oaaeeoey 3. ?aaaia?aoe?y ?ineei ?c ii?oiaaiiiai eaeono,

io?eiaiiai c a?a?eaeieo ca?iaee?a a?a/ee

Eiia?iaoe??

no?auoaaiue

E?euee?noue ca?iaee?a

*anoioa ?aaaia?aoe??, %

E?euee?noue ?aaaia?aio?a

Eiao?oe??io ?aaaia?aoe?? aenaae-aeaieoc ii?o.* eaeonii**Aeno.o E-17
502142,0 ± 0,8982,0Aeno.o E-21501224,0 ± 1,4460,9A.no. o E-17504488,0 ±
1,91142,3Aeii.A.oE-1750918,0 ± 0,4511,0 Cae. o E-17501632,0 ±
0,7971,9I?ei?oea: * — ii?oiaaiiei, ** — a?aeiioaiiy e?eueeino? io?eiaieo
?ineei-?aaaia?aio?a aei /enea aenaaeaeaieo ca?iaee?a.

A?aei?/aii caeaaei?noue /anoioe oa eiao?oe??ioa ?aaaia?aoe?? a?ae
aaiioeio. Oae o eiia?iaoe?yo Aiiinoee?eia o E-17, Aeno?a o E-17 ?
Caeaiiea?oeiaa o E-17, ye /anoioa ?aaaia?aoe?? (88%; 42%; 32%) oae ?
eiao?oe??io aoee aenieeie (2,3; 2,0; 1,9; a?aeiia?aeii), o ii??aiyii? c
eiia?iaoe?yie no?auoaaiue — Aeno?a o E-21, Aeiii? A?ei?onueeee o E-17
(oaae.3).

Ca aeiiiiiaith aeaiiai iaoiaeo aoei iaea?aeaii 406 ?ineei —
?aaaia?aio?a. Ii??aithth/e oe? aeai? c ?acoeueoaoaie, ye? ie iaee i?e
aei?iuoaaii? a?a?eaeieo ca?iaee?a, iiaeia i?eeoe aei aeniiaeo, ui aiie
aeinoia??ii a?ae??ciythoueny. O ia?a?aooieo ia eiaeai aenaaeaeaiee
ca?iaeie i?e eaeoniaaiac? ca?iaeea ? ?aaaia?aoe?? ?ineei ?c aeaio
ca?iaee?a iaea?aeaee — 2- 3 ?ineeie, a i?e aei?iuoaaii? — ?c aeaio —
iaeio.

Ioaea, e?euee?noue ?ineei-?aaaia?aio?a /a?ac eaeonio eoeueoo?o a aeaa
?ace ia?aaeuo? e?euee?noue ?ineei, iaea?aeaieo i?e aei?iuoaaii?
a?a?eaeieo ca?iaee?a.

Aiae?c a?a?eaeieo ?ineei a?a/ee. I?aeoaa?aeaeaiiy ?noeiiino?
i?aeaeaeiaeo a?a?eae?a a?a/ee i?iaiaeeee ca aeiiiiiaith iaoiaeo
?cioa?iaioiiai aiae?co. Aeine?aeaeoaaee aeoeai?noue 13 oa?iaioia:
IAAe-caeaaeii? iaeaoaeaaiae?iaaiace, IAAeO — caeaaeii?
iaeaoaeaaiae?iaaiace, IAAe*I aeaaiae?iaaiace, aeooaiaoaeaaiae?iaaiace,
aeeiaieueaeaaiae?iaaiace, noeoeeiaoaeaaiae?iaaiace,
6-oinoiaetheiiaoaeaaiae?iaaiace, oeeo?aoaeaaiae?i-aaiace, eenei?
oinoaoace, eoaeii? oinoaoace, aeiaoi?ace, ooia?ace, anoa?ace a eenoeao
? /a?aoeao aaouee?anueeeo oi?i oa a?a?eaeieo ?ineei a?a/ee, ui
ae?iuoaaeenue  in vitro. Anoaiiaeee, ui aeoeaieie a iiiaio
aeine?aeaeaiiy aoee — IAAe — caeaaeia iaeaoaeaa?ae?iaaiaca,
6-oinoiaethei-iaoaeaa?ae?iaaiaca, aeeiaieueaeaa?ae?iaaiaca.

A?aecia/eee, ui ?cioa?iaioi? niaeo?e aeeiaieueaeaa?ae?iaaiace
oa 6-oinoiaetheiiaoaeaa?ae?iaaiace a oeaieiao aaouee?anueeeo oi?i
i?noyoue ?cioi?ie oeeo oa?iaio?a ye? a?ae??ciythoueny ca
aeaeo?ioi?aoe/iith ?ooi-i?noth. O oaoa?nueei? a?a/ee F — oi?ia
oa?iaioo a?eueo ?ooiia, eoeueoo?-ii? — S — iaio ?ooiia. A?a?eaei?
?ineeie ia’?aeiothoue a nia? oe? aaouee?anuee? aeaeiniaoeeo?/i? oi?ie
(FS), ui i?aeoaa?aeaeo? ?o a?a?eaeio i?e?iaeo (iae. 1).

 Iae. 1. Aeaeo?ioi?aoe/i? niaeo?e aeeiaieueaeaa?ae?iaaiace o oeaieiao
aaouee?anueeeo aeae?a (eoeueoo?ia a?a/ea-ni?o Aeno?a, oaoa?nueea —
E-17), oa ?o i?aeaeaeiaeo a?a?eae?a (1,2,3).

Ioaea, oe? oa?iaioe iiaeooue neoaoaaoe ia?ea?ieie aeey a?aeai?o
ni?aaaei?o a?a?eaeieo ?ineei a?a/ee.

Ani iaea?aeai? 1014 ?ineei ?acii c aaoueeianueeeie oi?iaie aoee
i?iaiaeiciaaii ii aeaiei icioa?iaioai oa aeyaeai? ?c ieo 167 ?noeiieo.

I?iaaaeaii oeeoiaaiaoe/iee aiae?c 167 ni?aaaei?o  a?a?eaeieo ?ineei
a?a/ee. ?c 57 ?ineei, io?eiaieo iaoiaeii aei?iuoaaiiy ca?iaee?a, 43 iaee
aeeiei?aeiee iaa?? o?iiinii (2n=16), a o 14 aoei aeyaeaii aiaoiei?aeiee
iaa?? (o a?eueoino? aeiaaee?a 16±1 ?cio?iiiniia).

?c eaeonieo oeaiei ca?iaee?a iaea?aeaii 110 a?a?eaeieo ?ineei
-?aaaia?aio?a, na?aae yeeo 12 aoee aeeiei?aeieie, 62 —
oao?aiei?aeieie, 23 — aiaoiei?aeieie ? 13 i?eniiei?aeieie ii /eneo
o?iiinii.

*anoioa o?iiiniiieo aaa?aoe?e a ee?oeiao ia?enoaie ei?aiy aeeiei?aeieo
?ineei ia ia?aaeuoaaea 1,5%, a a ee?oeiao oao?aiei?aeieo
?ineei-?aaaia?aio?a ciaoiaeeeanue ia ??ai? 2%. Aiiiae?? i?oico aoee
i?aaenoaaeai? o aeaeyae? a?aenoaeeo o?iiinii, iino?a oa o?aaiaio?a.
Aeyaeai? aneiao?e/i? ? o?ueioiiethni? i?oice.

Iaea?aeaiiy aio?aeeiei?aeieo a?a?eaeieo ?ineei a?a/ee ?c oao?aiei?aeiei
iaai?ii o?iiinii ? iiaeieaiiyi ua iaeiiai aa?’??o i?ae-aeaeiai?
ianoi?niino? — noa?eeueiino? a?a?eae?a ia?oiai iieie?iiy.
Iie?iei?aeecaoe?y  ee?oei iiaeeeai aoea ?acoeueoaoii ae?? oiia
eoeueoeaoaaiiy oa ai?iiiaeueiiai neeaaeo iiaeeaiiai na?aaeiaeua ia
eaeoni? eoeueoo?e, io?eiai? ?c a?a?eaeieo ca?iaee?a. Ine?eueee ?ineeii?
ee?oeie caeaoi?  aei oioeiioaioiino?, oi iaea?aeai? ?c ieo
?ineeie-?aaaia?aioe iaee oao?aiei?aeiee iaa?? o?iiinii.

Oaeei /eiii, i?aenoiiaoth/e, ne?ae cacia/eoe, ui iaie cai?iiiiiaaia
noaia iiaoaiiiai iiaeieaiiy aa?’???a i?aeaeaeiai? ianoi?niino? o
a?a/ee yea ? aoaeoeaiei caniaii iaea?aeaiiy i?aeaeaeiaeo a?a?eae?a.

 NOAIA IAeA?AEAIIss I?AEAEAeIAEO A?A?EAe?A A?A*EE

Ooo/ia caieeaiiy c aeei?enoaiiyi ?ic/eio CdCl2 aeey iiaeieaiiy i?iaaiii?
ianoi?niino? (Ia?oee aa?’??)

Aeae?eaiiy ? eoeueoeaoaaiiy ca?iaee?a in vitro

(Ae?oaee aa?’?? — aiiiaeueiee ?icaeoie ca?iaee?a)  

I?yia aei?iuoaaiiy

ca?iaee?a (aio?aaiei?aee)Eaeoniaaiac ? ?aaaia?aoe?y aio?aeeiei?aeieo
?ineei  (O?ao?e aa?’ ?? — noa?eeuei?noue — a?a?eae?a F1 )

     

    

?ioaeoia ?ineeia

A?aea?? a?a?eaeieo ?ineei iaoiaeii ?cioa?iaioiiai aiae?co
aeeiaieueaeaa?ae?iaaiace ? 6-oinoiaetheiiaoaeaa?ae?iaaiace

A?a?eaei? ?ineeie

Iaea?aeaiiy o?ainaaiieo ?ineei a?a/ee oa ?o aiae?c .

?ic?iaeaiee iaoiae iaea?aeaiiy no?eeeo aei aa?a?oeeaeo
oino?iio?eoeeio ?ineei a?a/ee. Noeioeyoe?y i?yii? ?aaaia?aoe?? ?ineei
?c aenieaio?a caeaaeaea a?ae /ano ?o ia?iaee (iioeiaeueiee 2 aiaeeie)
ooaiii Agrobacterium tumefaciens pGV3850::pBin19/pBA3 c bar ? npt  II
aaiaie, neeaaeo na?aaeiaeua oa oiia eoeueoeaoaaiiy.

Iaeaeuo (14%) /anoioo iaaiiiooai?aiiy a?aecia/aee ia na?aaeiaeu? IN c
aeii?oeaie 3 ia/e AAI ? 0,1 ia/e ?IE. Iioeiaeueieie oiiaaie, i?ney
eieoeueoeaaoe?? aenieaio?a ?c ooaiii aa?iaaeoa???, aoei ae?iuoaaiiy
?o a oaiiiaiio oa?iinoao? i?e oaiia?aoo?? 27 0N, a c iiyaith
?aaaia?aio?a — ia na?oe? i?e 25±2 0N.

A?aea?? o?ainaaiieo ?ineei i?iaiaeeee ia na?aaeiaeu? c aeii?oeaie
100 ia/e eaiai?oeeio. No?ee? aei eaiai?oeeio iaaiie, ye? aoee
caeaieie ia?aiineee ia na?aaeiaeua c aeii?oeaie oino?iio?eoeeio a
eiioeaio?aoe?? 30 iee/e. A?aei?oeee, ui eiio?ieuei? ?ineeie aeioee
oaea i?e eiioeaio?aoe?? aa?a?oeeaeo 3 iee/e, a o?ainaaii? — i?e 30 iee/e
oei??ithaaeenue ? ?inee.

I?iaaaeaiee iieaeoey?ii — aiieiai/iee aiae?c ?aaaia?aio?a, iieacaa,
ui o?ainaaii? ?ineeie aeaee iiceoeaio aaoi?aae?ia?ao?/io
a?aeiia?aeue i?e aeio-a?a?eaeecaoe?? c i?/aiei ciiaeii, yeee iana bar
aai, ui na?ae/eoue i?i ?ioaa?aoe?th aeaiiai aaio a aaiii aeine?aeieo
?ineei (iae2.).

 Iae.2 a) ?ano?eeoe?eia ea?oa ieaci?aee pBA 3; a) aaoi?aae?ia?ao
 aeio-a?a?eaeecaoe?? ca Naoca?iii AeIE ?ineei a?a/ee; 1 — iiceoeaiee
eiio?ieue (Hind III — o?aaiaio ieaci?aee pBA 3 c aaiii bar);   2 — 6 —
AeIE ?ineei, a?ae?ie?ciaaieo ?ano?eeoacith Hind III, ye?  aeaee
iiceoeaio aaoi?aae?ia?ao?/io a?aeiia?aeue; 7 — iaaaoeaiee  eiio?ieue
(AeIE iao?ainaaiii? ?ineeie a?a/ee)

Oaiioei?/io aeni?an?th aaio aecia/eee o o?ainaaiieo ?ineei a?a/ee
ia?oiai iieie?iiy i?ney ia?iaee ?o eenoy eiia?oe?eiith eiioeaio?aoe??th
(3-5 ie/e) aa?a?oeeaeo. A?aecia/eee, ui a eiio?ieueieo ?ineei eenoy
ne?o/oaaeinue ? iiaaeaei, a o o?ainaaiieo caeeoaeinue caeaiei.

Ioaea, ?ic?iaeaiee iaie iaoiae o?ainoi?iaoe?? aeicaieea iaea?aeaoe
o?ainaaii? ?ineeie a?a/ee no?ee? aei aa?a?oeeaeo oino?iio?eoeeio.

AENIIAEE

 1.Aia?oa ?ic?iaeaiee iaoiae iiaoaiiiai iiaeieaiiy aa?’???a
i?aeaeaeiai? ianoi?niino? o a?a/ee, yeee aeicaiey? io?eioaaoe
a?a?eaei? ?ineeie.

 2. Iaea?aeaii aenieee a?aenioie (52-65%) ooai?aiiy caa’ycae o
aeine?aeaeoaaieo eiia?iaoe?yo no?auoaaiue oeyoii aeiai?o iioeiaeueii?
eiioeaio?aoe?? (0,1%) oei?enoiai eaaei?th.

 3.I?ae?a?ai? oiiae eoeueoeaoaaiiy, iioeiaeueiee neeaae iiaeeaiiai
na?aaeiaeua oa a?e aeae?eaiiy ca?iaee?a aeey aoaeoeaiiai ?o aei?iuoaaiiy
a oiiaao in vitro.

 4.Aecia/ai? noaae?? ?icaeoeo a?a?eaeiiai ca?iaeea oa neeaae iiaeeaiiai
na?aaeiaeua aeey iaea?aeaiiy ii?oiaaiiiai eaeono ? iioeiaeueii?
?aaaia?aoe?? ?c iueiai ?ineei.

 5. Anoaiiaeaii, ui oa?iaioe aeeiaieueaeaa?ae?iaaiaca ?
6-oinoiaetheiiao-aeaa?ae?iaaiaca iiaeooue neoaoaaoe ia?ea?ieie aeey
a?aeai?o ?noeiieo a?- a?eaeieo ?ineei a?a/ee.

 6.O?iiiniiiee aiae?c a?a?eaeieo ?ineei iieacaa, ui 74% ?ineei,
io?eiaieo i?e aei?iuoaaii? ca?iaee?a aoee aio?aaiei?aeieie, oiae?
ye a?a?eaee, iaea?aeai? /a?ac eaeonio eoeueoo?o, aoee a a?eueoino?
(57%) aio?aeeiei?aeieie.

 7.?ic?iaeaia iaoiaeeea o?ainoi?iaoe?? ?ineei a?a/ee, oeyoii i?yii?
?aaaia?aoe?? ?c aenieaio?a, yea aeicaiey? io?eioaaoe o?ainaaii?
?ineeie.

 8.Iayai?noue bar aaio a aaiii? o?ainaaiieo ?ineei i?aeoaa?aeaeaia
iaoiaeii aeio — a?a?eaeecaoe??.

 9.??aaiue oaiioei?/ii? aeni?an?? bar aaio o ?ineei aeicaieea ?i
aeo?eiaoe ia?iaeo eiia?oe?eiith eiioeaio?aoe??th aa?a?oeeaeo.
Aiaeia?/ia ia?iaea eiio?ieueieo ?ineei i?eaiaeeea aei ?o aeaoie?aoe??.

 NIENIE IIOAE?EIAAIEO INIIAIEO I?AOeUe

 1. ?oaoeiaa I.A., Eaaaiei A.A., Oa?aiaiei E.E., Oaiiaae A.E.
Iieo/aiea iaaeaeaeiauo aea?eaeia iaaeaeo a?a/eoie iaueiiaaiiie e
a?a/eoie oaoa?neie n iiiiuueth yia?eieoeueoo?u. // Oeceieiaey e aeioeiey
eoeueoo?iuo ?anoaiee. — 1994. — T 26, N6. — N. 563-566.

2. Rubtsova M.A.,  Levenko B.A., Oaranenko L.K., StekhinI.N., Zayats
A. Introduction of herbicide bialaphos resistance into buckwheat ?lants.
//Aeiiieeia?u e eeaoea.- 1997.- O.13, N5.- N.416-418.

 3. Oa?aiaiei E.E.,?oaoeiaa I.A.,Oaiiaae A.E,ssoeeoei I.E. Iieo/aiea
iaaeaeaeiauo aea?eaeia iaaeaeo  Fagopyrum esculentum e Fagopyrum
tataricum  n iiiiuueth aia?eieoeueoo?u. / / Naeaeoeey e oaoiieiaey
aicaeaeuaaiey iieaauo eoeueoo?. — *a?iiaoeu: I?oo.- 1994.- N. 113-118.

4. Eaaaiei A.A., Ia?uethoeei A.O., ?oaoeiaa I.A., Noaoei E.I.
O?ainaaiiua ?anoaiey onoie/eaua e aa?aeoeeaeai. — Iaoa?eaeu Iaaeaeoia?.
eiio. «Iieaeoey?iay aaiaoeea e aeioaoiieiaey». — Ieine: Aaea?onue. —
1998. — N. 217-219.

 5. Iao.98074048, Oe?a?ia ee.IEE6 A 01 ? 1/06. Niin?a iaea?aeaiiy
aio?aeeiei?aeieo oa?oeeueieo i?aeaeaeiaeo a?a?eae?a a?a/ee/ ?oaoeiaa
I.I., Eaaaiei A.I., Oa?aiaiei E.E. (Oe?a?ia); Cayae.3.07.98; Iioae.

 6. ?oaoeiaa I.A., Eaaaiei A.A., Oa?aiaiei E.E., Oaiiaae A.E.
I?aiaeieaiea iaaeaeaeiaie ianiaianoeiinoe o a?a/eoe. // Oac. aeiee. ??
Iaaeaeoia?. eiio.»Aeieiaey eeaoie ?anoaiee in vitro, aeioaoiieiaey e
nio?aiaiea aaiioiiaea». -Iineaa (?inney).- 1997. — N.157.

 7. Rubtsova M.A., Levenko B.A., Taranenko L.K. Fertile
interspecific amphidiploid hybrids of buckwheat. // Abstr. of the II
Int. Symp. on Plant Biotechnology . — Kyiv (Ukraine). — 1998. — P.109.

?oaoeiaa I.I. Iaea?aeaiiy i?aeaeaeiaeo a?a?eae?a ? o?ainaaiieo ?ineei
a?a/ee oa ?o aiae?c. — ?oeiien.

  Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa 
a?ieia?/ieo iaoe ca niaoe?aeuei?noth 03.00.15-aaiaoeea. — ?inoeooo 
ee?oeiii? a?ieia?? ? aaiaoe/ii? ?iaeaia???  IAI Oe?a?ie, Ee?a, 1998.

Aeena?oaoe?th i?enay/aii ?ic?iaoe? iaoiae?a io?eiaiiy i?aeaeaeiaeo
a?a?eae?a ? o?ainaaiieo ?ineeie a?a/ee. Iioei?ciaai? oiiae aei?iuoaaiiy
a?a?eaeieo ca?iaee?a ? ?aaaia?aoe?? ?ineei ?c eaeonii? oeaieie.
Aecia/ai? ia?ea?i? oa?iaioe aeey a?aeai?o ?noeiieo a?a?eaeieo ?ineei
a?a/ee. Iieacaii, ui iaea?aeai? iaoiaeii ?aaaia?aoe?? ?c eaeono
a?a?eaeieo ca?iaee?a ?ineeie, ? a a?eueoino? aeiaaee?a
aio?aeeiei?aeaie. ?ic?iaeaia iaoiaeeea o?ainoi?iaoe?? a?a/ee oeyoii
i?yii? ?aaaia?aoe?? ?c aenieaio?a. Iaea?aeai? i?aeaeaeia? a?a?eaee ?
o?ainaaii? ?ineeie a?a/ee c ainiiaea?nueei — oe?iieie iciaeaie.

Eeth/ia? neiaa : a?a/ea, i?aeaeaeia? a?a?eaee, eaeoni? eoeueoo?e,
aio?aeeiei?aee , o?ainaaii? ?ineeie.

?oaoeiaa I.A. Iieo/aiea iaaeaeaeiauo aea?eaeia e o?ainaaiiuo
 ?anoaiee a?a/eoe e eo aiaeec. — ?oeiienue.

Aeenna?oaoeey ia nieneaiea noaiaie  eaiaeeaeaoa aeieiae/aneeo iaoe
ii niaoeeaeueiinoe 03.00.15- 0  aaiaoeea. — Einoeooo  eeaoi/iie aeieiaee
e aaiaoe/aneie eiaeaia?ee  IAI Oe?aeiu, Eeaa, 1998.

Aeenna?oaoeey iinayuaia ?ac?aaioea iaoiaeia iieo/aiey iaaeaeaeiauo
aea?eaeia e o?ainaaiiuo ?anoaiee a?a/eoe. Iioeiece?iaaiu oneiaey
aei?aueaaiiey aea?eaeiuo ca?iaeuoae e ?aaaia?aoeee ?anoaiee ec
eaeeoniie oeaie. Ii?aaeaeaiu ia?ea?iua oa?iaiou aeey ioai?a enoeiiuo
aea?eaeiuo ?anoaiee a?a/eoe. Iieacaii, /oi iieo/aiiua iaoiaeii
?aaaia?aoeee ec eaeeona aea?eaeiuo ca?iaeuoae ?anoaiey, yaeythony a
aieueoeinoaa neo/aaa aioeaeeieieaeaie. ?ac?aaioaia iaoiaeeea
o?ainoi?iaoeee a?a/eoe iooai i?yiie ?aaaia?aoeee ec yenieaioia.
Iieo/aiu iaaeaeaeiaua aea?eaeu e o?ainaaiiua ?anoaiey a?a/eoe n
oicyenoaaiii — oeaiiuie i?eciaeaie.

Eeth/aaua neiaa : a?a/eoa, iaaeaeaeiaua aea?eaeu, eaeeoniua eoeueoo?u,
aioeaeeieieaeu, o?ainaaiiua ?anoaiey.

 Rubtsova M.A. Obtaining  the interspecific  hybrids and
transgenic buckwheat plants and their analysis. — Manuscript.

Thesis for Ph.D. degree (Biology) by speciality 03.00.15. —
genetics. — Institute of Cell Biology and Genetic Engineering National
Academy of Sciences of Ukraine, Kyiv, 1998.

The thesis is devoted to the development of the methods of
obtaining interspecific hybrids and transgenic buckwheat plants. The
conditions of  hybrid embryos growth and plant regeneration from
their callus tissue have been optimized. The marker enzymes for the
selection of true hybrids of buckwheat have been determined. Plants
regenerated from callus tissues developed from hybrid embryos were
mainly amphidiploids. The method of genetic transformation of
buckwheat plants by direct regeneration from primary explants was
worked out. The interspecific hybrids and transgenic buckwheat
plants with agriculturally valuable characters have been obtained.

 Key words : buckwheat, interspecific  hybrids , callus tissues,
amphidiploid, transgenic plants.

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