IAOe?IIAEUeIA AEAAeAI?ss IAOE OE?A?IE

?INOEOOO O?C?IEIA?? ?I.I.I.AIAIIIEUeOess

Ia i?aaao ?oeiieno

NAA*AIEI AAEAIOEIA EAIIO??AIA

OAeE 612.822

I?E?IAE?AEUeI? EE?OEIE IICEO UO??A A

IIOIAAIAC? OA I?E O?AAEAII? IA?AIAI? OEAIEIE.

03.00.13-O?cieia?y ethaeeie ? oaa?ei

AAOI?AOA?AO

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa a?ieia?/ieo iaoe

EE?A — 1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia o a?aeae?e? oeeoieia??

?inoeoooo o?c?ieia?? ?i.I.I.Aiaiiieueoey IAI Oe?a?ie

Iaoeiaee ea??aiee: i?ioani? IAI Oe?a?ie, aeieoi? iaaee/ieo iaoe

Ne?ai Aaeeia A?eai??aia

?inoeooo o?c?ieia?? ?i.I.I.Aiaiiieueoey IAI Oe?a?ie

caa. a?aeae?eii oeeoieia??

Io?oe?ei? iiiiaioe: aeieoi? a?ieia?/ieo iaoe Eooai?ia Ieaia Iaae?aia,

?inoeooo o?c?ieia???
?i.I.I.Aiaiiieueoey IAI Oe?a?ie.

aeieoi? iaaee/ieo iaoe *aeeianueeee
TH??e Aiaaeaiiae/,

Iaoe?iiaeueiee iaaee/iee

oieaa?neoao ?i.I.I.Aiaiiieueoey,
ea??aiee a?aeae?eii.

I?ia?aeia onoaiiaa: ?inoeooo aa?iioieia?? AII Oe?a?ie.

Caoeno a?aeaoaeaoueny “___16__”aa?aciy_1999 ?. o”___14___”aiaeei? ia
can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee i?e ?inoeooo? o?c?ieia??
?i.I.I.Aiaiiieueoey IAI Oe?a?ie ca aae?anith: 252024, i.Ee?a,
aoe.Aiaiiieueoey 4.

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? ?inoeoooo o?c?ieia??
?i.I.I.Aiaiiieueoey IAI Oe?a?ie ca aae?anith: 252024, i.Ee?a,
aoe.Aiaiiieueoey 4.

Aaoi?aoa?ao ?ic?neaiee “__08___”_ethoiai_1999 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee

aeieoi? a?ieia?/ieo iaoe Ni?ie?ia-Ia??ia C.I.

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue i?iaeaie. I?e?iae?aeuei? ee?oeie ? iaeiei ?c oei?a
ae?aeueieo ee?oei a oeaio?aeuei?e ia?aia?e nenoai? (OeIN), aiie
oa?aeoa?ecothoueny iniaeeaith ii?oieia??th oa aeeiiothoue o?io?/io,
nae?aoi?io, oaaioeeoa?io oa ?io? ooieoe??. I?ioyaii aeeooy
i?e?iae?aeuei? ee?oeie iathoue ??ci? ii?oieia?/i? oa?aeoa?enoeee ?
ianooue ??cia ooieoe?iiaeueia iaaaioaaeaiiy a caeaaeiino? a?ae noaio
iiceo. Aea/aiiy i?e?iae?aeueieo ee?oei a i?ioean? iioiaaiaco iieacaei,
ui i?ioyaii ?icaeoeo iiceo i?e?iae?aeuei? ee?oeie caciathoue oe?eee
?yae oaiioeiiaeo ia?aoai?aiue: aiaai?aeia i?e?iae?y i?a?o? ?c
noaaaio?eeoey?ii? ciie o an? ?aa?iie ? aeeoa?aioe?th?oueny a
?icaaeoaeaio i?e?iae?th, yea ?iciiae?ey?oueny a?aeiinii ??aiii??ii ii
an?e oeaiei? iiceo.

A?aeiii, ui a i?ioean? ?icaeoeo ia?aiai? oeaieie, i?e?iae?y ni?ey?
aeaeeaaiith iae?ii?a oa ?ioeo ee?oei iiceo. I?e ??cieo iaoieia?/ieo
noaiao iiceo i?e?iae?aeuei? ee?oeie a?aeiia?aeathoue ia o?aaeaiiy
aeoeaaoe??th ? aeni?an??th aioeaai?a IIN eean?a 1 ? 2, nae?aoe??th
oeeoie?i?a (IL-1, TNF) (Buttini M. et al.,1996) ? oaeoi??a ?inoo (NGF,
NT-3, TGF-() (Elkabes S. et al., 1996, Dobbertin A. et al., 1997). I?e
aeaaaia?aoe?? iae?ii?a oa ?ioeo ee?oei iiceo i?e?iae?aeuei? ee?oeie
aeeiiothoue oaaioeeoa?io ooieoe?th. Iieacaii, ui a i?ioean? ?icaeoeo
iiceo, i?e eiai ii?iaeueiiio ooieoe?iioaaii? a c??eiio noai? oa i?e
??cieo iaoieia?/ieo aieeaao a?aeaoaa?oueny aca?iiae?y i?e?iae?aeueieo
ee?oei c iae?iiaie oa ?ioeie ae?aeueieie ee?oeiaie, aea ooieoe??
i?e?iae?? a oae?e aca?iiae?? caeeoathoueny ia aei e?ioey aea/aieie.

Aei oaia??oiueiai /ano i?e?iae?aeuei? ee?oeie inoathoueny iaeiaiueo
aea/aieie a iiceo a ca’yceo c o?oaeiiuaie a ?o ?aeaioeo?eaoe??.
A?aeaoaa?oueny iino?eiee iiooe niaoeeo?/iiai ia?ea?a i?e?iae?aeueieo
ee?oei, yeee ae ci?a aaeaeaaoii aeyaeyoe anth ee?oeiio iiioeyoe?th
i?e?iae?? ? aea/aoe iioiaeaeaiiy ? ooieoe?? oeeo ee?oei a ii?i? ? i?e
??cieo iaoieia?/ieo noaiao ia?aiai? oeaieie. Ia nueiaiaeiyoi?e aeaiue
aeey aeyaeaiiy i?e?iae?aeueieo ee?oei aeei?enoiaothoue ??ci? ia?ea?e, a
naia: NR3 (complement type 3 receptor) (Perry V.H., 1994), ae?eii?ioa?i
F4/80 (Lawson L.J. et al., 1990), ?cieaeoei GSA I-A4 (?c Griphonia
Simplicifolia (GSA)) (Streit W.J. et al., 1990), oinoioe?icei (PT) (Karp
H.L. et al., 1994). Caaaeyee aioeo?eai aei oeeo aioeaai?a ? iiaeeea?noue
?aeaioeo?eoaaoe iiioeyoe?th i?e?iae?? a OeIN. Iaeiae, i?iaeaia iieyay? a
oiio, ui iaa?aeiii /e any iiioeyoe?y i?e?iae?? ia? oe? aioeaaie, aai
aeai?i/ii ?aeaioeo?eo?oueny o?eueee iaaia /anoeia i?e?iae??aeueieo
ee?oei.

Iaaeaaii aoei aeyaeaii, ui e?iiei?oei 1 (LC1) (I.i. 35000) —
ae?eii?ioa?i, ? ia?ea?ii i?e?iae?? a iiceo (Ahluwalia A. et al., 1996).
LC1 ca’yco?oueny c oinoaoeaeeena??iaie iaia?ai a i?enooiino? Na++ ? ?
noano?aoii ?aoeaioi?a ai?aea?iaeueiiai oaeoi?a ?inoo ca oe?iceiii,
iaae?aoi?ii aetheiei?oeei?ae?a. LC1 ia? i?ioecaiaeueio ae?th, ?ia?aoth/e
aeoeai?noue oinoie?iace A ? oei naiei ooai?aiiy i?inoaaeaiaeei?a ye
iniiaiiai aeaea?aea caiaeaiiy (Raynal P. et al., 1994). Ine?eueee LC1
aoa aeyaeai a i?e?iae?? ? aioeo?ea aei iueiai ?ioaineaii caaa?aethaaee
yae?i, oeeoiieacio ? a?ae?inoee, aaaeeeai aoei aea/eoe /e i?noeoue eiai
any iiioeyoe?y i?e?iae??, a oaeiae /e ? oeae ia?ea? aaeaeaaoiei aeey
aea/aiiy iiaaae?iee i?e?iae?? ia ??cieo aoaiao ?icaeoeo iiceo e?en.

Aeey a?eueo aeaoaeueiiai aea/aiiy ooieoe?iiaeueiiai cia/aiiy i?e?iae??
a c??eiio iiceo iaiao?aeii i?iaiae?coaaoe oiiia?ao?/iee ?iciiae?e
i?e?iae?? a ??cieo ?aa?iiao, a oaeiae iaea?aeaoe e?euee?noi?
oa?aeoa?enoeee oeeo ee?oei a ??cieo ii ooieoe?iiaeueiiio cia/aii?
iae?iiaeueieo no?oeoo? iiceo, e??i oeueiai /a?aci??ii aaaeeeaei ?
a?aeiioaiiy i?e?iae?? ? ano?ioeeo?a a oeeo no?oeoo?ao. Iaeia ?c
i?iiiiiaaieo a?iioac iieyay? a oiio, ui ae?y ?aaoeth? e?euee?noue
neiaioe/ieo eiioaeo?a, caeia? iaai? oa??eoi??? ia iiaa?oi? iae?ii?a, ye?
iiaee aooe caeiyo? eeoi?ie neiainaie, ae?y caaania/o? ooieoe?iiaeueio
noaa?euei?noue neiaioe/ii? ia?ai?caoe?? ? o/anoao? a oi?ioaaii? ia?aiai?
ae?yeueiino?.

A caeaaeiino? a?ae a?aeiia?ae? i?e?iae?? ia o?aaeaiiy ??cii? aoeieia??
iiaeia aecia/eoe eieae?caoe?th ina?aaeea o?aaeaiiy oa nooi?iue o?aaeaiiy
iae?ii?a. Iaoai?ci aca?iiae?? i?e?iae??, ano?ioeeo?a oa ae?oaeo ee?oei
iiceo i?e ??cieo iaoieia?yo ia aei e?ioey aea/aiee, a oaeiae i?e/eie, ui
aeceaathoue i?ie?oa?aoe?th ae??. Iaei??th ?c iiaeeeaeo i?e/ei aeoeaaoe??
i?e?iae?? ? oinoi?eethaaiiy a?ee?a ca oe?iceiii. Ne?aeo? i?aee?aneeoe,
ui LC1 ? noano?aoii ?aoeaioi?a ai?aea?iaeueiiai oaeoi?a ?inoo ca
oe?iceiii ? oiio iniaeeai aaaeeeai ii??aiyoe a?aeiia?aeue LC1-iiceoeaieo
(LC1+), ? ?O+ aeoeaiaaii? i?e?iae?? i?e iaoieia??.

Ia a?aei?io a?ae i?e?iae?? ii?oieia?y oa ooieoe?? ano?ioeeo?a aea/ai?
iaaaaaoi a?eueoa. Ano?ioeeoe i?aaenoaaeai? ee?oeiaie c??/anoi? oi?ie,
ye? i?eeiathoue o/anoue a i?a?aoe?? ia?aiaeo ee?oei ? i?aeo?eiothoue ?o
aiiainoac, a oaeiae o oi?ioaaii? aaiaoiaioeaoae?/iiai aa?’??o. A
caeaaeiino? a?ae nai?o ii?oieia?/ieo oa?aeoa?enoee ano?ioeeoe
iiae?eythoueny ia aieieieno? oa i?ioiieaciaoe/i?. Aei neeaaeo i?ii?aeieo
o?eaiaio?a oeeoineaeaoo ano?ioeeo?a aoiaeeoue ae?aeueiee o?a?eey?iee
eeneee a?eie, yeee ? ia?ea?ii oeeo ee?oei. Aioo??oiueiee?oeiiee
ae?eii?ioa?i S100( oaeiae aeei?enoiao?oueny ye ia?ea? aeey iiioeyoe??
ano?ioeeo?a (Ghandour M.S. et al., 1981). O?aaeaiiy iiceo ??cii?
i?e?iaee i?ecaiaeeoue aei ?aaeoeaiiai ano?ioeeoico — eieaeueiiai
ca?eueoaiiy /enea ? ?ici???a ano?ioeeo?a, i?e oeueiio /anoi
noai?th?oueny ano?ioeeoa?iee ?oaaoeue. Ni?eueii c i?e?iae??th ano?ioeeoe
oi?iothoue ?ioiio a?aeiia?aeue a OeIN.

Aeine?aeaeaiiy i?iaiaeeeenue a?aeiia?aeii aei ieai?a iaoeiai? ?iaioe
a?aeae?eo oeeoieia?? ?inoeoooo o?c?ieia?? ?i.I.I.Aiaiiieueoey IAI
Oe?a?ie.

Iaoa ? caaea/? aeine?aeaeaiiy.

Iaoith ?iaioe aoei aea/aiiy iniaeeainoae ii?oiaaiaco i?e?iae?aeueieo
ee?oei ? ?iciiae?e i?e?iae?? a i?ioean? iioiaaiaco, aeei?enoiaoth/e
aenieo niaoeeo?/i?noue aioeo?e aei LC1, iaa?oioiaaio iaoiaeaie
ii??aiyeueiiai e?euee?noiiai aiaeeco, aeaoe ii??aiyeueio e?euee?noio
oa?aeoa?enoeeo ?iciiae?eo i?e?iae?? oa ano?ioeeo?a a ??cieo ?aa?iiao
c??eiai iiceo a ii?i? oa i?e aenia?eiaioaeuei?e iaoieia?? ??cii?
aoeieia??.

A ca’yceo c oeei a caaea/? ?iaioe aoiaeeei:

1. Aea/eoe niaoeeo?/i?noue LC1 ye ia?ea?a i?e?iae??.

2. Ii??aiyoe ?iciiae?e LC1+, PT+, GSA+ i?e?iae?aeueieo ee?oei a ??cieo
?aa?iiao iiceo.

3. Aea/eoe ii?oiaaiac oa ?iciiae?e LC1+ i?e?iae?? ia c??cao iiceo a
i?ioean? iioiaaiaco, aeei?enoiaoth/e aeey oeueiai a?noieia?/iee oa
?ioiia?noio?i?/iee iaoiaee.

4. Ii??aiyoe ?iciiae?eaiiy iiioeyoe?e i?e?iae?? oa ano?ioeeo?a a ??cieo
iaeanoyo c??eiai iiceo aeey a?eueo aeaoaeueiiai aea/aiiy ooieoe?e ?
aca?iiae?? oeeo ee?oei a c??eiio iiceo a ii?i?.

5. Aea/eoe a?aeiia?aeue i?e?iae?? oa ano?ioeeo?a ia o?aaeaiiy iiceo
??cii? aoeieia??, a naia, i?e aeiaaeuei?e ei?ioei/ani?e ?oai??,
ia?a??coe? i?aei/iiyieiaiai ia?ao oa i?e aieea? eaca?iei ii?ii?iaiiyi ia
nainiiioi?io ei?o.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a.

A aeena?oaoe?ei?e ?iaio? ioa?aeoa?eciaaii LC1 ye niaoeeo?/iee ia?ea?
an?o cono??/athueony a ia?aia?e nenoai? oi?i (aiaai?aeii?, i?ii?aeii?,
?icaaeoaeaii? oa aeoeaiaaii?) i?e?iae??, i?iaaaeaii ii??aiyiiy
iiioeyoe?e i?e?iae??, aeyaeaieo ??cieie ia?ea?aie: e?iiei?oeiii-1,
oinoioe?iceiii, ?cieaeoeiii GSA IB4. I?iaaaeaii e?euee?niee aiae?c
?iciiae?eo i?e?iae?aeueieo ee?oei oa ni?anoaaeai? oiiia?ao?/i? ea?oe
eieae?caoe?? i?e?iae?? a ??ci? ia??iaee ?icaeoeo iiceo uo??a, a c??eiio
noai? oa i?e iaoieia?yo ??cii? i?e?iaee.

A ?iaio? i?noeoueny ?yae iiaeo aenia?eiaioaeueieo aeaieo a?aeiinii
?aaeoeaieo aeanoeainoae i?e?iae?aeueieo ee?oei i?e o?aaeaii? oeaieie
iiceo. Anoaiiaeaii, /oi i?e o?aaeaiiyo ??cii? aoeieia?? i?e?iae?aeuei?
ee?oeie ci?iththoue nai? oaiioeiia? iciaee, ui na?ae/eoue i?i ?o
aeoeaaoe?th, aeyaeythoueny oa?aeoa?i? iniaeeaino? oiiia?ao?/iiai
?iciiae?eo aeoeaiaaii? i?e?iae??. Oae, i?e iaoai?/iiio o?aaeaii?
i?aei/iiyieiaiai ia?ao aeyaeaia a?aeiia?aeue aeoeaiaaii? i?e?iae?? a
noiaao?? iiceo a yae?ao o??e/anoiai ia?ao — iaeano? ia?aeiii?
neiaioe/ii? ia?aaea/? neaiae?a i?e ia?a??coe? i?aei/iiyieiaiai ia?ao.
Iieacaii, i?e aenia?eiaioaeuei?e ?oai?? aeoeaiaaia i?e?iae?y
neoi/o?oueny a?ey iaea?eueo /ooeeaeie aei ?oai?? i??ai?aeieo iae?ii?a a
NA1 iaeano? a?iieaiio. I?ney o?aaeaiiy ei?e aieiaiiai iiceo eaca?iei
ii?ii?iaiiyi aeoeaiaaia i?e?iae?y eiioeaio?o?oueny a?ey o?aaeaieo
ei?oeeaeueieo iae?ii?a.

S100(-ae?eii?ioa?i aeei?enoiaoaaee ye ia?ea? aeey ano?ioeeo?a. Aeey
e?euee?niiai ii??aiyeueiiai aiae?co ?iciiae?eo i?e?iae?? oa ano?ioeeo?a
o ??cieo ?aa?iiao iiceo uo??a aeei?enoiaoaaee noaoenoe/ii-noa?aieia?/iee
i?aeo?ae oa niaoe?aeueio eiii’thoa?io i?ia?aio, yea aeicaieeea
iaea?aeaoe oiiia?ao?/i? ea?oe ?iciiae?eo ae?? a ??ci? ia??iaee ?icaeoeo,
a c??eiio iiceo oa i?e iaoieia??.

Oai?aoe/ia oa i?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a.

Iaea?aeai? ?acoeueoaoe cia/ii aeiiiaiththoue no/ani? ciaiiy i?i
ii?oiaaiac i?e?iae?aeueieo ee?oei a i?ioean? iioiaaiaoe/iiai ?icaeoeo,
?o ?iciiae?e a ??cieo iaeanoyo iiceo. E??i oiai, iieo/ai? aeai? aeathoue
iiaeeea?noue aiae?coaaoe a?aeiioaiiy ? aca?iiae?th i?ae ee?oeiaie
ano?ioeeoa?ii? ae?? oa i?e?iae??, a oaeiae ioe?ieoe ?aaeoeai?
aeanoeaino? i?e?iae?? oa ano?ioeeo?a i?e iaoieia?/ieo noaiao ia?aiai?
oeaieie ??cii? aoeieia??.

Iaea?aeai? aeai? i?aaenoaaeythoue cia/iee ?ioa?an ? a i?aeoe/iiio
ieai?. Cie?aia, anoaiiaeaiee oaeo i?i oa, ui LC1 yaey?oueny aoaeoeaiei
ia?ea?ii, yeee iaea?eueo iiaii aeyaey? i?e?iae?th, aeicaiey?
?aeiiaiaeoaaoe oeae i?aia?ao ia o?eueee aeey ?ioiia?noio?i?/iiai aiae?co
?ioaeoii? ia?aiai? oeaieie, aea ? aeey iaoiii?oieia?/iiai aiae?co
naeoe?eiiai iaoa??aeo a iae?io??o?a?/i?e i?aeoeoe? c iaoith
?aeaioeo?eaoe?? ae?aeueieo iooeei iiceo oa ?ioeo ina?aaee?a o?aaeaiiy
iiceo, ui noi?iaiaeaeothoueny eiioeaio?aoe??th i?e?iae?aeueieo ee?oei
(i?e caiaeueieo, o?aaiaoe/ieo, ?oai?/ieo, iaiieanoe/ieo i?ioeanao).
I?aaenoaaeaiee ii?oiiao?e/iee iaoiae aeine?aeaeaiiy ae?aeueieo ee?oei
iiaea aooe aeei?enoaiee a ?o iiaeaeueoiio aea/aii?.

Iniaenoee aianie caeiaoaa/a.

Caeiaoaa/ai iniaenoi aeeiiai? o?c?ieia?/i?, iaoio?c?ieia?/i? oa an?
?ioiia?noio?i?/i? aenia?eiaioe, aiae?c ? iiaoaeiaa oiiia?ao?/ieo ea?o
oa ia?aooaaiiy aeaieo i?e aeiiiiic? BIOQUANT i?ia?aie. Caeiaoaa/ a?aa
aeoeaio o/anoue o iaaiai?aii? ?acoeueoao?a oa oi?ioethaaii? aeniiae?a.
Aia?oa caeiaoaa/ai iieacaii, ui ia ?aii?o aoaiao iioiaaiaco LC1+
i?e?iae?y i?a?o? ?c noaaaio?eeoey?ii? ciie ? a c??eiio ii?iaeueiiio
iiceo ?iciiae?ey?oueny a a?eueo?e e?eueeino? a ii??aiyii? c ?ioeie
ia?ea?aie. A?aeiia?aeue LC1+ oa ?O+ aeoeaiaaii? i?e?iae?? aoea
?aeaioe/iith a yae?ao o??e/anoiai ia?ao i?ney ia?a??cee i?aei/iiyieiaiai
ia?ao. Aia?oa iieacaii, ui LC1+aeoeaiaaia i?e?iae?y neoi/o?oueny a NA1
cii? a?iieaiio. Aoea i?iaiae?ciaaia ?aaeoe?y i?e?iae?? ? ano?ioeeo?a
i?ney eaca?iiai ii?ii?iaiiy.

Ai?iaaoe?y ?iaioe.

Iniiai? iieiaeaiiy ?iaioe aeiiia?aeaeenue ? iaaiai?thaaeenue ia
i?aeia?iaei?e eiioa?aioe?? a A?aoe?? (European Meeting on Glial Cell
Function in Health and Disease.May 6-10, 1998 Athens, Greece), ia XV
c’?cae? O?c?ieia?a a Aeiiaoeueeo (Iaoa??aee XV C‘?caeo Oe?a?inueeiai
O?c?ieia?/iiai Oiaa?enoaa) 1998, ia 1 (Onoaiia/?e) Eiioa?aioe?? a Ee?a?
(Oe?a?inueea Oiaa?enoai Iae?iiaoe. 24-26 eenoiiaaea 1998).

Ioae?eaoe??. Ca iaoa??aeaie aeena?oaoe?? iioae?eiaaii /ioe?e noaoo? a
iaoeiaeo aeo?iaeao ? oace i’yoe aeiiia?aeae.

No?oeoo?a oa ianya aeena?oaoe??. Aeena?oaoe?y neeaaea?oueny c? anooio,
iaeyaeo e?oa?aoo?e, iaoa??ae?a oa iaoiae?a aeine?aeaeaiiy, ?acoeueoao?a
aeine?aeaeaiue, iaaiai?aiiy ?acoeueoao?a, aeniiae?a oa nieneo
aeei?enoaieo aeaea?ae c? 182 iaeiaioaaiue. ?iaioa aeeeaaeaia ia 120
noi??ieao oa ?ethno?iaaia 28 ?enoieaieoa iaei??th oaaeeoeath.

INIIAIEE CI?NO AeENA?OAOe??

Iaoa??aee oa iaoiaee aeine?aeaeaiue.

?iaioa aoea i?iaaaeaia ia 82 uo?ao e?i?? Long-Evans(NOA). Aea/aee iicie
??ciinoaoaaeo uo??a o i?ioean? iinoiaoaeueiiai ?icaeoeo — ia 1, 7, 15 oa
21 aeaiue oa a c??eiio noai?. Aeey aea/aiiy ?aaeoe?? i?e?iae?? ia
o?aaeaiiy aoee noai?aii? ??ci? ii i?e?iae? o?aaeaiiy iiceo a naia:
aeiaaeueia ?oai?y, ia?a??cea i?aei/iiyieiaiai ia?ao oa o?aaeaiiy ei?e
aieiaiiai iiceo uo??a eaca?iei ii?ii?iaiiyi. Iiaeaeue aeiaaeueii? ?oai??
noai?thaaeanue a?aeiia?aeii iienaiiio iaoiaeo (Reid K.H. et al., 1996),
i?e eio?iio i?ney ia?eioecoaaiiy eaoai?iii (50 ia/ea) o uo??a
caeaaethaaee a?oaeio ee?oeo i?ioyaii 7-10 oaeeei c iine?aeoth/ith
ea?aeeinoeioeyoe??th aeey a?aeiiaeaiiy aeeoaiiy oa na?oeaaeooy. Iicie
aea/aee ia 30-oee aeaiue i?ney o?aaeaiiy.

O aeiaaeeo ia?a??cee i?aei/iiyieiaiai ia?ao uo??a ia?eioecoaaee
eaoai?iii oa ia?a??caee ia?a o iaeano? i?aei/iiyieiaiai ioai?o (Melzer
P. et al., 1997). Iicie aea/aee /a?ac 4-?e aeiy i?ney iia?aoe??.

Aeey noai?aiiy o?aaeaiiy ei?e uo??a ia?eioecoaaee iaiaooaeii (40 ia/ea),
o?enoaaee aieiao ? ?iaeee o?aiaiaoe?th /a?aia. I?aeaeaaaee eaca?iiio
ii?ii?iaiith iaeanoue nainiiioi?ii? ei?e ii iaoiaeo Edwards G. et al.,
(1994) (aeiaaeeia oaee? 4 iei, iae?i?iioeuenia aia?a?y 22
i?e?Aeaeioey/iae?i?iioeuen, 16 oi/ie, aeiaaeeia eaca?iiai oeyoo 1 ni) ?
oeaieio iiceo aea/aee /a?ac 3 oa 6 aei?a i?ney o?aaeaiiy.

O?enaoe?y oeaieie.

Aeey aea/aiiy ?iciiae?eo i?e?iae?? a c??cao iiceo uo??a ie
aeei?enoiaoaaee iiaeeo?eiaaiee ?ioiia?noio?i?/iee iaoiae (McKanna J.A.
et al., 1997).

Ia?eioeciaaieo uo??a nii/aoeo ia?oocoaaee o?ainea?ae?aeueii
o?c?ieia?/iei ?ic/eiii a ia’?i? 100 ie, ui i?noea aaia?ei (1000 iae/ea)
oa 0.01% i?o??oo iao??th, a iio?i — o?enoth/ei ?ic/eiii GPAS ia’?iii
250 ie, ui i?noea 2.5% aethoa?aeueaeaa?aeo, 0.01M iaoa-ia?eiaeaoo
iao??y, 0.1M oei?eaeo iao??y, 0.04M oinoaoo iao??y, 1% ioeoiai? eeneioe
(?I=4). C?aco ae i?ney ia?ooc?? iicie aeeo/aee oa aeiaeaoeiai o?enoaaee
a GPAS i?ioyaii 5 aiaeei i?e e?iiaoi?e oaiia?aoo??. Aeey an?o aeiaaee?a
?ioiia?noio?i?/iiai caaa?aeaiiy ee?oei aeei?enoiaoaaany o?eueee GPAS
o?enaoi?. Ca?aeii aeaieo McKanna J.A. et al. (1997), oeae o?enaoi?
aeicaiey? aeyaeyoe LC1 in situ, caaacia/oth/e oei naiei niaoeeo?/i?noue
?ioiia?noio?i?/iiai caaa?aeaiiy ee?oei i?e?iae?? aioeo?eaie aei oeueiai
ae?eii?ioa?io. GPAS aeei?enoiaoaaany aeey o?enaoe?? oeaieie o an?o
aeiaaeeao ?ioiia?noio?i?/iiai aiae?ca. I?ney aeaa?ae?aoaoe?? oeaieio
iinoo?enoaaee a 100% nie?o? i?ioyaii 12 aiaeei i?e 40 N oa caeeth/aee a
ia?ao?i. Ia iiia?a/ieo c??cao iiceo uo??a aoee aea/aii? ii?oieia?y oa
?iciiae?eaiiy i?e?iae?aeueieo ee?oei oa ano?ioeeo?a a ia?aaeiueiio,
na?aaeiueiio oa caaeiueiio iiceo.

?ioiia?noio?i?/iee aiae?c.

Aeey ?ioiia?noio?i?/iiai caaa?aeaiiy iie?eeiiaeueieie e?iey/eie
aioeo?eaie i?ioe LC1 (1:8000) (Ahn N.G. et al., 1988), ?O (1:1000)
(Zymed, NOA), S100( (1:4000) (Van Eldik L.J. et al., 1988)
aeei?enoiaoaaee aeaia?ao?iiaai? c??ce iiceo oiaueiith 10 iei.
?ia?aoaaiiy aiaeiaaiii? ia?ieneaeace i?iaiaeeee 0.3% ia?ieneaeii
aiaeith. Aeieoaaiiy ianiaoeeo?/iiai ca’ycoaaiiy cae?enithaaeinue ca
aeiiiiiaith 10% ii?iaeueii? eiceeii? ne?iaaoee ia i?ioyc? 30 oaeeei,
i?ney oeueiai c??ce ?ieoaoaaee a ?ic/ei? ia?aeiieo aioeo?e i?ioyaii 12
aiaeei i?e 4O N. Aoi?eiieie aioeo?eaie aoa a?ioei?eiaaiee eiceeiee
aioe-e?iey/ee IgG (H+L) (Vector Laboratories). O?aoeiieie aioeo?eaie
neoaeea eiiieaen aa?aeei-a?ioei-ia?ieneaeaca (ABC kits, Vector
Laboratories). O?iiaoiaaiii a oe?e ?aaeoe?? yaeyany ae?ai?iiaaiceaeei
(DAB), a aeey caaa?aeaiiy ee?oei aeei?enoiaoaaee oieo?aeeiiaee nei?e.

sse eiio?ie? aoei aeei?enoaii c??ce iiceo, ?ieoaiaai? c: 1)
ii?iaeueiith e?iey/ith ne?iaaoeith; 2) ?c niaoe?o?/ieie e?iey/eie
aioeo?eaie, aeieiaaieie i/euaieie aioeaaiaie; 3) aac e?iey/eo aioeo?e. O
an?o eiio?ieueieo aeiaaeeao caaa?aeaiiy ia niinoa??aaeinue.

Ine?eueee iaoith oe??? ?iaioe aoei aeyaeaiiy i?e?iae?aeueieo ee?oei ca
aeiiiiiaith ??cieo oeeoio?i?/ieo iaoiae?a aeine?aeaeaiiy, iaie aoa
aeei?enoaiee oaeiae ?cieaeoei GSA I-B4, yeee niaoe?o?/ii ca’ycoaaany c
aeueoa-D-aaeaeoiceeiaeie caeeoeaie iieaeoe ae?eieae?eno oeeo ee?oei (GSA
I-B4 -HRP — Griffonia simplicifolia B4-?cieaeoei-ia?ieneaeaciee
eiiieaen) (Sigma L5391, NOA) (Streit W.J., 1990). Aeaia?ao?iiaai? c??ce
iiceo ?ieoaoaaee c ?cieaeoeiii (20 iea/ie) a caaooa?iaaiiio
o?c?ieia?/iiio ?ic/ei? Aeoeueaaeei i?ioyaii 12 aiaeei. O?iiaoiaaiii a
oe?e ?aaeoe?? oaeiae neoaeea DAB.

Noaoenoe/ia ia?iaea.

Caaa?aeai? c??ce niinoa??aaee i?ae i?e?ineiiii (Ortholux, Leitz,
Wetzlar, Germany). Aeey aiae?co ?iciiae?eo i?e?iae?aeueieo ee?oei oa
ano?ioeeo?a ? iiaoaeiae oiiia?ao?/ieo ea?o aeei?enoiaoaaee eiii’thoa?io
nenoaio BIOQUANT OS/2 (R&M Biometrics, Nashville, USA).

Ei?aeoe?y ?acoeueoao?a c aeei?enoaiiyi iaoiaeo Abercrombie M. (1940)
aeicaieeea ioe?ieoe ?noeiio e?euee?noue ee?oei ia iaeeieoeth ieiu?.

?acoeueoaoe aeine?aeaeaiue.

Aoei iieacaii, ui a iiceo iiaiia?iaeaeaieo oaa?ei LC1+ ee?oeie
aiaai?aeii? i?e?iae??, iaee ie?oaeo aaca?ae?ino/anoo oi?io, ?iiae? ia ?o
iiaa?oi? niinoa??aaeenue inaaaeiiiae??. Ae?aiao? ee?oei a na?aaeiueiio
neeaaeaa 10 i?e?ii. Aiaai?aei? ee?oeie i?e?iae?? aoee neiioeaio?iaai? a
noaaaio?eeoey?i?e cii? a?ey aiaiae?iaeueieo ee?oei eaoa?aeueieo
oeoii/e?a; a ?ioeo iaeanoyo iiceo aeyaeyeenue eeoa iaeeie/i?
?icaaeoaeai? ee?oeie. Na?aaeiy e?euee?noue aiaai?aeieo ee?oei i?e?iae??
ia ia?oee aeaiue iinoiaoaeueiiai ?icaeoeo noaiiaeea 31(8 ee?oei/ii2.

A iiceo 7-aeaiieo uo?aiyo aeey LC1+ i?e?iae?aeueieo ee?oei aoee
oa?aeoa?i? iciaee «i?ii?aeiiai» oeio, ui yaeyeinue iane?aeeii
ia?aoai?aiiy aiaai?aeii? i?e?iae?? a ?? ?icaaeoaeaio oi?io. Ee?oeie iaee
aiaai?aeia o?ei oa e?eueea inaaaeiiiae?e — ei?ioeeo, iioiauaieo
a?ae?inoe?a; ae?aiao? ee?oei ia ia?aaeuoaaa 8-9 i?e?ii. A oeae ia??iae o
noaaaio?eeoey?i?e cii? niinoa??aaeanue cia/ia e?euee?noue aiaai?aeieo
ee?oei. Niinoa??aaeanue i?a?aoe?y «i?ii?aeieo» ee?oei ?c
noaaaio?eeoey?ii? ciie a ??ci? ?aa?iie iiceo. Na?aaeiy e?euee?noue LC1+
ee?oei ia anueiio c??c? ia?aaeiueiai iiceo a oeae /an ?icaeoeo noaiiaeea
54(15 ee?oei/ii2.

Ia 15 aeaiue iinoiaoaeueiiai ?icaeoeo iiceo LC1+ i?e?iae?y
ciaoiaeeeanue a i?ioean? aeeoa?aioe?thaaiiy. O?ei ee?oei iaei iaaaeee?
?ici??e, ae?aiao? niie ia ia?aaeuoaaa 6-8 i?e?ii, a?ae?inoee aoee
oiieeie ? a cia/i?e i??? ?icaaeoaeaieie. Ia oeueiio aoai? ?icaeoeo
?iciiae?e ee?oei i?e?iae?? aoa a?aeiinii ??aiii??iei ii anueiio iiceo,
niinoa??aaeinue eeoa iacia/ia i?aeaeuaiiy aonoioe ee?oei o
noaaaio?eeoey?i?e cii?. Na?aaeiy e?euee?noue LC1+ ee?oei aoea 104(20
ee?oei/ii2.

Ia 21 aeaiue iinoiaoaeueiiai ?icaeoeo LC1+ ee?oeie i?e?iae?? aoee
i?aaenoaaeai? o?eueee ?icaaeoaeaiith oi?iith, aonoioa aoea iiae?aiith
aei LC1+ ?icaaeoaeaii? i?e?iae?? a c??eiio iiceo. O?ei ee?oei aoei
ie?oaea aai iaaeueia c aeiaaeie, oiieeie, ?icaaeoaeaieie a?ae?inoeaie.
?iciiae?eaiiy LC1+ i?e?iae?aeueieo ee?oei aoei a?aeiinii ??aiii??iei a
a?e?e ?a/iaei? ocaeiaae aenii?a oa a n???e ?a/iaei?.

?iciiae?eaiiy LC1+ i?e?iae?? a c??eiio iiceo.

A c??eiio iiceo uo??a LC1+ ee?oeie i?e?iae?? aoee i?aaenoaaeai?
?icaaeoaeaiith oi?iith. A n???e ?a/iaei? o?ei ee?oei aoei e?oaea, c
aeiaaeie, oiieeie, ?aae?aeueii ?icaaeoaeaieie a?ae?inoeaie, a a a?e?e
?a/iaei? niia aoea iaaeueiith, c aeae?eueeaie a?ae?inoeaie,
?icoaoiaaieie oaeiaae aenii?a. Na?aaeiy e?euee?noue LC1+ ?icaaeoaeaii?
i?e?iae?? a oe?eiio iiceo c?noaaeyea 103±9 ee?oei/ii2 (Oaaeeoey).
Iaeiae, aonoioa i?e?iae?aeueieo ee?oei ci?ithaaeanue a caeaaeiino? a?ae
aeine?aeaeaii? iaeano?. A n???e ?a/iaei? aonoioa ee?oei aoea aeuith
ii??aithth/e c a?eith. Na?aaeiy e?euee?noue LC1+ ee?oei aoea
iaea?eueoith a iaino??aooi?, aeae?eueea iaiueoith — a a?iioaeaion? oa
ei?? aieiaiiai iiceo. Iecea aonoioa LC1+ i?e?iae?? aoea a a?iieaii?,
oaeaion? oa iaeiaiueoa e?euee?noue niinoa??aaeanue o iici/eo ? noiaao??
iiceo (Oaaeeoey).

Aeey ii??aiyeueii? ioe?iee cae?aiino? ??cieo ia?ea??a aeyaeyoue
i?e?iae?aeuei? ee?oeie iaie aoa aeei?enoaiiee niaeo? iie?eeiiaeueieo
aioeo?e, niaoeeo?/ieo ii a?aeiioaiith aei iee?iae??.

?iciiae?eaiiy ?O+ i?e?iae??.

Aioeo?ea aei ?O aeia?a caaa?aethaaee o?ea i?e?iae?aeueieo ee?oei oa
neaai caaa?aethaaee a?ae?inoee. A n???e oa a?e?e ?a/iaei? ii?oieia?y ?O+
i?e?iae?? aoea oaeith ae aaoa?iaaiiith, ye ? o LC1+ ee?oeiao. ?O+
i?e?iae?aeuei? ee?oeie a?aeiinii ??aiii??ii ?iciiae?eyeenue ii anueiio
iiceo. Na?aaeiy e?euee?noue ?O+ ee?oei aoea 54±5 ee?oei/ii2. I?aeaeuaia
aonoioa ?O+ i?e?iae?? niinoa??aaeanue o eiai?e oa o?i’yi?e iaeanoyo ei?e
aieiaiiai iiceo. Iaeiaiueoa e?euee?noue ?O+ i?e?iae?? a?aei?/aia a
ai?eaeuei?e oa eaoa?aeuei?e /anoeiao iaino??aooia, oioy a eiai
iaae?aeuei?e /anoei? /enei ee?oei aoei iaea?eueoei.

?iciiae?eaiiy GSA+ i?e?iae??.

I?e?iae?aeuei? ee?oeie a c??eiio iiceo neaai caaa?aethaaeenue eaeoeiii ?
?o aonoioa aoea iaaaaaoi ieuith, ii??aithth/e c LC1+ oa ?O+ i?e?iae??th.
Na?aaeiy e?euee?noue GSA+ ee?oei c?noaaeyea 34±2 ee?oei/ii2. Oaeei
/eiii, iaie aoei i?iaaaeaii ea/anoaaiiay oa e?euee?noia ioe?iea
?iciiae?eaiiy i?e?iae?aeueieo ee?oei a aieiaiiio iiceo.

Uia a?ae??cieoe iiioeyoe?? LC1+ i?e?iae?? a?ae ano?ioeeo?a
ii??aithaaeinue ?iciiae?eaiiy i?e?iae?? oa ano?ioeeo?a a ??cieo iaeanoyo
iiceo.

?iciiae?eaiiy S100(+ ano?ioeeo?a a ii?iaeueiiio c??eiio iiceo.

Aioeo?ea aei S100( aeinoaoiuei ?ioaineaii caaa?aethaaee yae?a
ano?ioeeo?a, iaeiae, neaai caaa?aethaaee oeeoiieacio oa a?ae?inoee
ee?oei. Oi?ia ee?oei ia a?ae??ciyeanue a ??cieo iaeanoyo iiceo.

Na?aaeiy e?euee?noue S100(+ ano?ioeeo?a a oe?eiio iiceo aoea 217±30
ee?oei/ii2, i?e/iio a a?e?e ?a/iaei? ?o aoei a?eueoa, /ei a n???e.
Aaeeea e?euee?noue ano?ioeeo?a aoea aeyaeaia a a?iieaii? oa
a?iioaeaion?, a iaeiaiueoa — a ei?? aieiaiiai iiceo (ca aeeeth/aiiyi
iioeei/ii? iaeano?), iici/eo oa aeiaaanoiio iiceo (Oaaeeoey). A
iaino??aooi? aonoioa ano?ioeeo?a oaeiae aoea iecueeith, ca aeeeth/aiiyi
iaae?aeueii? iaeano?, aea aonoioa oeeo ae?aeueieo ee?oei aeinyaaea
230±19 ee?oei/ii2. Aonoioa eieae?caoe?? ano?ioeeo?a a ??cieo ciiao iiceo
aoea cia/ii a?eueoith, i?ae aonoioa i?e?iae?aeueieo ee?oei. sseui
a?aeeeiooe cia/aiiy oeueiai ia?aiao?o a iici/eo oa aeiaaanoiio iiceo
(aea aonoioa i?e?iae?? i?i?iaeueia), oi a?aie/i? cia/aiiy aonoioe LC1+
i?e?iae?aeueieo ee?oei a?ae??ciyeenue i?eaeecii a 1.5 ?ace, a oae? ae
cia/aiiy aeey S100(+ ano?ioeeo?a — a 2.1 ?aca.

Ii??aiyeueiee aiae?c ?iciiae?eo aeoeaiaaii? i?e?iae?? oa ?aaeoeaieo
ano?ioeeo?a i?e ei?ioei/ani?e aeiaaeuei?e ?oai??.

Ia 30-oee aeaiue i?ney ?oai?? niinoa??aaeinue neoi/aiiy LC1+
aeoeaiaaii? i?e?iae?? a NA1 cii? a?iieaiio. Iaea?eueoa aonoioa oeeo
ee?oei a?aei?/aeanue iaaeiei niie i??ai?aeieo iae?ii?a, ocaeiaae
aeaiae?eo?a ? aenii?a. Na?aaeiy e?euee?noue LC1+ i?e?iae?aeueieo ee?oei
a a?iieaii? i?ney ?oai?? noaiiaeea 2010(6 ee?oei/ii2. A ii?i? ae LC1+
i?e?iae?aeuei? ee?oeie ?iciiae?eyeenue a a?iieaii? a?aeiinii ??aiii??ii,
? ?o aonoioa aoea cia/ii ieuith (94(9 ee?oei/ii2).

Iaaaeeea neoi/aiiy S100(+ ?aaeoeaieo ano?ioeeo?a niinoa??aaeinue a
iieaeoey?iiio oa?? CA1 iaeano? a?iieaiio ii??aithth/e c eiio?ieai.
I?ioa, a ?ioeo iaeanoyo a?iieaiio ?iciiae?e ?aaeoeaieo ano?ioeeo?a i?e
?oai?? ia a?ae??ciyany a?ae eiio?ieth ? na?aaeiy e?euee?noue
ano?ioeeo?a neeaaeaea 244(8 ee?oei/ii2 i?ney ?oai?? (241(26 ee?oei/ii2 a
eiio?ie?).

Oe? aeai? na?ae/aoue i?i oa, ui i?e ?oai?? eieae?caoe?y oa
i?ie?oa?aoeaia a?aeiia?aeue i?e?iae?? oa ano?ioeeo?a a?ae??ciythoueny.
Aeoeaiaaia i?e?iae?y, a iniiaiiio, eieae?co?oueny a?ey niie i??ai?aeieo
iae?ii?a, oei /anii ye ano?ioeeoe — a?ey neiaioe/ieo cae?i/aiue a
iieaeoey?iiio oa??.

A?aeiia?aeue aeoeaiaaii? i?e?iae?? ia ia?a??ceo i?aei/iiyieiaiai ia?ao.

I?aei/iiyieiaee ia?a ? a?eeith o??e/anoiai ia?ao, oiio i?ney eiai
ia?a??cee ia ia?eoa??? niinoa??aaeanue a?aeiia?aeue LC1+ oa ?O+
i?e?iae?? a yae?ao o??e/anoiai ia?ao. Ia 4 aeaiue i?ney ia?a??cee
i?aei/iiyieiaiai ia?ao niinoa??aaeinue neoi/aiiy LC1+ aeoeaiaaii?
i?e?iae?? a iaeano? yaea? o??e/anoiai ia?ao noiaao?a iiceo ia noi?ii?
o?aaeaiiy. Ia a?eueoiio ca?eueoaii? aoei aeaeii, ui oe? ee?oeie iaee
niio a?eueoeo ?ici???a oa ei?ioe? iioiauai? a?ae?inoee. Aonoioa LC1+
aeoeaiaaii? i?e?iae?? neeaaeaea 405(22 ee?oei/ii2 ia noi?ii? o?aaeaiiy a
iaeano? yaea? o??e/anoiai ia?ao. Ia i?ioeeaaei?e noi?ii? ?o aonoioa aoea
cia/ii ieae/ith ? neeaaeaea 72(4 ee?oeie/ii2.

Iiae?aia ea?oeia niinoa??aaeanue i?e aeei?enoaii? aioeo?e aei ?O.
Eeanoa??caoe?y ?O+ aeoeaiaaii? i?e?iae?? oaeiae a?aeaoaaeanue a iaeano?
yaea? o??e/anoiai ia?ao. Oi?ia ?O+ aeoeaiaaii? i?e?iae?? aoea iiae?aiith
aei LC1+ aeoeaiaaii? i?e?iae??. Aonoioa ?O+ aeoeaiaaii? i?e?iae?? ia 4
aeaiue i?ney iia?aoe?? neeaaeaea 242(15 ee?oei/ii2 ia noi?ii? o?aaeaiiy,
a ia i?ioeeaaei?e noi?ii? — 68(8 ee?oei/ii2.

Oaeei /eiii, ?aaeoe?y LC1+ oa ?O+ aeoeaiaaii? i?e?iae?? iii?oia oaea ia
2-4 aeiao i?ney iia?aoe?? ia noi?ii? o?aaeaiiy a yae?ao o??e/anoiai
ia?ao. E?euee?noue i?e?iae?aeueieo ee?oei ooo a 3-5 ?ac?a a?eueoa a
ii??aiyii? c i?ioeeaaeiith noi?iiith. I?e oeueiio LC1+ ee?oei
aeyaey?oueny a?eueoa, i?ae ?O+ ee?oei, oiaoi ia any iiioeyoe?y LC1+
aeoeaiaaii? i?e?iae?? ? ?O-iiceoeaiith. Iiaeeeai, ui a?aeiia?aeue LC1+
aeoeaiaaii? i?e?iae?? i?ney ia?eoa?e/iiai o?aaeaiiy ia?ao ni?yaeaia c
oinoi?eethaaiiyi a?ee?a ca oe?iceiii, cie?aia, LC1.

A?aeiia?aeue ae?? ia o?aaeaiiy ei?e aieiaiiai iiceo eaca?ii.

Ii??aithaaeinue neoi/aiiy i?e?iae?? oa ano?ioeeo?a iaaeiei o?aaeaiii?
ciie /a?ac 3 oa 6 ae?a i?ney o?aaeaiiy.

*a?ac 3 aeiy i?ney o?aaeaiiy ei?e neoi/aiiy LC1+ aeoeaiaaieo
i?e?iae?aeueieo ee?oei niinoa??aaeinue a i?noe? o?aaeaiiy. Na?aaeiy
e?euee?noue i?e?iae?? a ei?? ia noi?ii? o?aaeaiiy neeaaeaea 149(37
ee?oei/ii2, ii??aithth/e c eiio?ieai (112(6 ee?oei/ii2).

A oeae ia??iae iacia/ia e?euee?noue S100+ ?aaeoeaieo ano?ioeeo?a
neoi/oaaeanue ia iaae? ina?aaeeo oa caei?iai? oeaieie. I?ioa,
e?euee?noue ano?ioeeo?a a ei?? ia noi?ii? o?aaeaiiy neeaaeaea 133(18
ee?oei/ii2, ii??aithth/e n eiio?ieai (147(5 ee?oei/ii2).

*a?ac 6 aei?a LC1+ aeoeaiaaia i?e?iae?y iaea aeaeyae a?eueo eouoaaoi? ?
?? aonoioa a ei?? ia noi?ii? o?aaeaiiy cia/ii c?inoaea (318(40
eeaoie/ii2), ii??aithth/e c eiio?ieai (93(15 ee?oei/ii2).

A oeae ia??iae oaeiae niinoa??aaeinue iaaaeeea neoi/aiiy S100+
?aaeoeaieo ano?ioeeo?a ia iaae? ina?aaeeo iioeiaeaeaiiy oa caei?iai?
oeaieie ? e?euee?noue a ei?? ia noi?ii? o?aaeaiiy aoea 196(32
ee?oei/ii2, ii??aithth/e c eiio?ieai (148(5 ee?oei/ii2).

A?aeiia?aeue i?e?iae?aeueieo ee?oei ia o?aaeaiiy ei?e iiceo eaca?ii
i?iyaey?oueny ?o aeoeaaoe??th oa i?ie?oa?aoe??th a ia?o? aei? i?ney
aieeao. Ano?ioeeoa?ia ?aaeoe?y niinoa??aaeanue a iaiueoiio nooiai? oa
i?ci?oa.

Iaaiai?aiiy ?acoeueoao?a aeine?aeaeaiue.

A ianoiyuiio aeine?aeaeaii? aoei aeyaeaii, ui a i?ioeann? ?icaeoeo
iiceo a?aeaoaathoueny ia o?eueee aeeiai?/i? ci?ie ii?oieia?/ieo
aeanoeainoae i?e?iae?aeueieo ee?oei, aea e ci?ith?oueny ?o oiiia?ao?/ia
i?aai?caoe?y.

A inoaiiueiio aeanyoe??//? aeey aeyaeaiiy i?e?iae?aeueieo ee?oei
aeei?enoiaothoue aioeaaie iae?ioaa?a, oae? ye NR3 (Perry V.H., 1994),
F4/80 aioeaai (Lawson L.J. et al., 1990), CD45 oa ?O (Karp H.L. et al.,
1994), a oaeiae aeey aeyaeaiiy i?e?iae?? aeei?enoiaoaaee GSA ?cieaeoei
B4 (Streit W.J. et al., 1990). Ia iniia? aeine?aeaeaiiy ca aeiiiiiaith
caaaeueieo ia?ea??a aeey i?e?iae?? iiceo oa iae?ioaa?a ?ioeo oeaiei oa
iayaiino? iiae?aieo ? ?aeaioe/ieo ?aoeaioi??a ia oeeo ee?oeiao ?
caeaoi?noue cae?enithaaoe oaaioeeoa?io ooieoe?th, aaoi?e
noaa?aeaeothoue, ui i?e?iae?y, iae?ioaae oa iiiioeeoe e?ia? iathoue
caaaeueiiai iiia?aaeieea.

Iaeiae, i?e oaeineiiaeaii? iaoiaeeee o?enaoe?? a i?e?iae?? aoa
aeyaeaiee LC1, 35 eAea ae?eii?ioa?i, iaeiith ?c eiai i?ioecaiaeueiith
aeanoeainooth aoei ?ia?aoaaiiy ooai?aiiy a?ao?aeiiiai? eeneioe. Oeae
ae?eii?ioa?i aoa a?aenooi?i a iae?iiao, ano?ioeeoao oa
ie?aiaeaiae?ioeeoao iiceo. A i?ei?oeaieo ae?aeueieo ee?oeiao na?aaeeiii?
e?i?? aacaeueii? iaia?aie ia?aiai? o?oaee ? a aeayeeo aiaiae?iaeueieo
ee?oeiao oeoii/e?a i?ioyaii aia??iiaeueiiai ? ?aiiueiai iinoiaoaeueiiai
?icaeoeo oaeiae ciaoiaeeoueny LC1 ye ? a i?e?iae?? a iinoiaoaeueiiio
iiceo (McKanna J.A., 1993). Iiaeeeai, ui i?ei?oeai? ae?aeuei? ee?oeie ?
aiaiae?iaeuei? ee?oeie yaeythoueny iiia?aaeieeaie i?e?iae?aeueieo
ee?oei.

?c aeua iaaaaeaieo ?acoeueoao?a ne?aeo?, ui ia ??cieo yoaiao ?icaeoeo
iiceo i?e?iae?y aeyaey?oueny o aeaeyae? ??ciiiai?oieo ca ii?oieia?/ieie
oa?aeoa?enoeeaie ee?oei: aiaai?aeii? oi?ie c oeeoiieaciaoe/ieie
aeieiaiiyie, i?ii?aeii? oi?ie c ei?ioeeie iioiauaieie a?ae?inoeaie ?
?icaaeoaeaii? i?e?iae??, eio?a aeyaey?oueny o c??eiio iiceo. Oe? aeai?
ocaiaeaeothoueny c aeaieie Streit oa ?ioeie, ye? i?e aeei?enoaii?
?cieaeoeia GSA I-A4 (?c Griphonia Simplicifolia) oaeiae aeyaeyee
aiaeia?/i? ia?aoai?aiiy aiaai?aeii? i?e?iae?? ? iiaeaeueoa ??
aeeoa?aioe?thaaiiy (Streit W.J. et al., 1988; Streit W.J., 1990).

Oae, ?iaeoueny ia?aaeaa/aiiy, ui ?iciiae?e iae?ioaa?a ? i?e?iae?? a
iicieenoiio o?e? o iiaiia?iaeaeaiiio iiceo iiaea aooe iia’ycaiee c
?inoii aenii?a i?ioyaii oi?ioaaiiy ia?ao?anoiiai aeniiaeueiiai oeyoo, /e
c e?ea?aeaoe??th iae?ii?a, ye? ia onoaiiaeee eiioaeoa c ?ioeie iae?iiaie
(Innocenti G.M. et al.,1983; Iversen L. et al., 1994).

Iaie aoei iieacaii, ui a i?ioean? iinoiaoaeueiiai ?icaeoeo (?1-?15)
i?ii?aeia i?e?iae?y ?iciianthaeaeo?oueny a?ae noaaaio?eeoey?ii? ciie ii
anueiio iiceo. Iiaeia i?eionoeoe, ui i?e?iae?aeuei? ee?oeie o aeaeyae?
i?ii?aeii? oi?ie c iioiauaieie, ei?ioeeie inaaaeii?aeeaie i?a?othoue ii
anueiio iiceo ? aeeoa?aioe?ththoueny aei 15 aeiy iinoiaoaeueiiai
?icaeoeo. Hurley ? Streit i?e aeei?enoaii? ?cieaeoeia GSA aeae?eythoue
o?ainoi?iiaaio i?e?iae?th ye i?ii?aeiee oei i?e ia?aoai?aii? aiaai?aeii?
oi?ie a ?icaaeoaeaio i?e?iae?th (Hurley S.D. and Streit W.J., 1996). Ca
iaoeie aeaieie aiaai?aeia i?e?iae?y i?ie?oa?o? ? i?a?o? ?c
noaaaio?eeoey?ii? ciie ii anueiio iiceo ? aeeoa?aioe?th?oueny a
?icaaeoaeaio i?e?iae?th. Ne?ae cai?oeoe, ui ia i?ioyc? ia??iaeo ?icaeoeo
iiceo aonoioa i?e?iae?? aoea aeuith a a?e?e ?a/iaei? a iaeano?
eaoa?aeueieo oeoii/e?a.

Ca iaoeie aeaieie LC1 ye ia?ea? i?e?iae?? aeicaiey? iaea?eueo iiaii
aeyaeoe iiioeyoe?th oeeo ee?oei a c??eiio iiceo. Aei oaia??oiueiai /ano
ooieoe?? ?icaaeoaeaii? i?e?iae?? a c??eiio iiceo iaaeinoaoiuei a?aeii? ?
aeey a?eueo aeaoaeueiiai aea/aiiy i?e?iae?? aoei a aeioe?eueii
iiaoaeoaaoe oiiia?ao?/i? ea?oe aeey aea/aiiy iiioeyoe?? ?icaaeoaeaii?
i?e?iae?? c aeei?enoaiiyi a?aeiinii iiaiai ia?ea?a LC1 ? ii??aiyiiyi c
?ioeie ia?ea?aie. ?O ye ia?ea? i?e?iae?? aoa aea?aiee aeey oiai, uia
aea/eoe i?ioeane oinoi?eethaaiiy a?ee?a ii oe?iceio a ?icaaeoaeai?e ?
aeoeaiaai?e i?e?iae??. I?e ii??aiyii? iiioeyoe?e LC1+ ? PT+
i?e?iae?aeueieo ee?oei aoei iieacaii, ui e?euee?noue LC1+
i?e?iae?aeueieo ee?oei a?eueoa a ii??aiyii? c PO+ ee?oeiaie. I?e/eiith
oeueiai iiaea aooe oa, ui a?eee ia an??? iiioeyoe?? ?icaaeoaeaii?
i?e?iae?? yaeythoueny oinoi?eeueiaaiieie ii oe?iceio. Aeey LC1+ ? ?O+
i?e?iae?aeueieo ee?oei, aeyaeaieo a n???e ?a/iaei?, aoea oa?aeoa?ia
ie?oaea niia ? ?aae?aeueii ?icaaeoaeai? a?ae?inoee; a a?e?e ?a/iaei? —
iaaeueii? oi?ie o?ei ? aeae?eueea a?ae?inoe?a, ?icoaoiaaieo acaeiaae
aenii?a. Oae ye a n???e ?a/iaei? nae?aoo?oueny aaeeea e?euee?noue
a?ieia?/ii aeoeaieo ?a/iaei, iiaeia i?eionoeoe, ui aiie noeioeththoue
??no a?ae?inoe?a i?e?iae??, ui i?eaiaeeoue aei ca?eueoaiiy iiaa?oi? ?o
eiioaeo?a c niiith iae?ii?a, ?ioeie ee?oeiaie ? ioi/oth/ei
na?aaeiaeuai. A a?e?e ?a/iaei?, aea ciaoiaeyoueny o?eueee i??e?i?ciaai?
aeniie, oi?ia ? e?euee?noue i?e?iae?? nooo?ai a?ae??ciy?oueny, ui
ocaiaeaeo?oueny c aeaieie Lawson ? Streit ?c ni?aaaoi?aie (Lawson L.J.
et al., 1990; Streit W.J., 1990).

Aiae?c ?ioiia?noio?i?/iiai caaa?aeaiiy iieacaa, ui ii?oieia?/i?
iniaeeaino? i?e?iae??, aeyaeaii? ca aeiiiiiaith aioeo?e aei
??ciiiai?oieo ia?ea??a oa ?cieaeoeia, aeinoaoiuei noiae?, iaeiae,
e?euee?noue caaa?aeaieo ee?oei aoea iaea?eueoith i?e aeei?enoaii? LC1
(?<0.05). Ie c?iaeee i?eiouaiiy, ui LC1, ia a?aei?io a?ae ?ioeo ia?ea??a, yaey?oueny iaea?eueo aaeaeaaoiei ia?ea?ii aeey aeyaeaiiy iiioeyoe?? i?e?iae??, ? oea aeicaiey? a?eueo ia’?eoeaii aeine?aeaeoaaoe ?? a ii?iaeueiiio iiceo. I?inoi?iaee ?iciiae?e iiioeyoe?? i?e?iae?? nooo?ai a?ae??ciyany a?ae ?iciiae?eo ano?iae??, aonoioa ee?oei yei? a aeae?eueea ?ac ia?aaeuoaaea aiaeia?/iee iieaciee i?e?iae??. Ca iaoeie aeaieie, i?e?iae?y, yea ia?aaoaa? a niiei?, ?icn?yia a?aeiinii iaeii??aeii a iiceo, oi?ioth/e n?oue ?ioiiaoaeoi?ieo ee?oei, ye? iiaeooue aeoeaoaaoeny i?e o?aaeaii? ia?aiai? nenoaie, a??oni?e ?ioaeoe?? /e iaoai?/i?e o?aai? (Karp H.L. et al., 1994; Milligan C.E. et al., 1991). I?ae?aoiaaia iaie na?aaeiy e?euee?noue LC1+ ee?oei a iiceo uo??a aoea ia 32% a?eueoith, /ei aeyaeaia Lawson c? ni?aaaoi?aie a iiceo ieo? c aeei?enoaiiyi ia?ea?a F4/80 (Lawson L.J. et al., 1990). Ca iaoeie aeaieie, iaea?eueoa e?euee?noue i?e?iae?? i?noeoueny a iaino??aooi?. Oea iiaea aooe iia'ycaii c nae?aoe??th aeioai?ia?a?/ieo iae?iiaae?aoi??a a oeueiio ?aa?ii? iiceo (Lawson L.J. et al., 1990). Aea/ath/e F4/80+ i?e?iae?th, aaoi?e ia aeyaeee caeaaei?noue ?? aonoioe a?ae e?eueeino? iae?ii?a,ui caaeioee a ??cieo ?aa?iiao ii?iaeueiiai iiceo ieo?, ui aeicaiey? i?eionoeoe, ui neoi/aiiy i?e?iae?? a c??eiio iiceo a ii?i? iiaeooue aooe iia'ycai? ia no?eueee c oaaioeeoa?iith, ne?eueee c ?ioeie ooieoe?yie i?e?iae?aeueieo ee?oei (Lawson L.J. et al., 1990). I?aeoaa?aeaeaiiyi oeueiai i?eiouaiiy iiaea oaeiae neoaeeoe a?aenooi?noue /?oei? ei?aeyoe?? i?ae aonoioith ano?ioeeo?a ? i?e?iae?? a ??cieo ?aa?iiao iiceo (Oaaeeoea). Iaie aoei aeyaeaii, ui ano?ioeeoe ?iciiae?eai? a ??cieo ?aa?iiao iiceo iaio ??aiii??ii, i?ae i?e?iae?y. Iaea?eueoa ?o e?euee?noue ciaoiaeeoueny a a?e?e ?a/iaei?, a ithoia?e ? iioeee/i?e ei?? aieiaiiai iiceo, a?iioaeaion? ? coa/ano?e ai?ici? a?iieaiio. Iiaeeeai, aca?iiae?y ano?ioeeo?a c ie?aiaeaiae?ioeeoaie, i?enooi?ie a a?e?e ?a/iaei?, aieeaa? ia i?ie?oa?aoe?th ia?oeo (Schweizer M. et al., 1993). A a?e?e ?a/iaei? ano?ioeeoe a?eueo ?ioaineaii caaa?aeai?, a ca'yceo c /ei iiaeia i?eionoeoe, ui a oe?e /anoei? iiceo eiioeaio?aoe?y S100( a ano?ioeeoao aeua. Neoi/aiiy ano?ioeeo?a a iaeano? a?iioaeaiona, iiaeeeai, iia'ycaii c iae?iaiaeie?eiiith ooieoe??th aeacaiiiai a?aeae?eo iiceo. Oaeei /eiii, iiaeia i?eionoeoe, ui ??cia aonoioa ano?ioeeo?a ? i?e?iae?? a ??cieo ?aa?iiao c??eiai iiceo iiaea caeaaeaoe a?ae ?ie?, ui aeeiio?oueny oeeie ee?oeiaie a aeaieo iaeanoyo iiceo, a?ae oei?a iae?ii?a, a?ae iae?iiaae?aoi??a, ui aeae?eythoueny ieie, a oaeiae a?ae ooieoe?iiaeueieo iniaeeainoae ie?aieo ?aa?ii?a iiceo. I?e iaoieia?/ieo noaiao iiceo ae?aeuei? ee?oeie ?aaaothoue ia caaeaaeue iae?ii?a oa ia ci?ie eiioeaio?aoe?? nae?aoo?ieo iae?iiaae?aoi??a, i?ie?oa?aoe??th oa oaaioeeoicii. Uia aecia/eoe ci?ie c aieo ae??, iaiao?aeii aea/eoe ?noeiio e?euee?noue i?e?iae?? ? ano?ioeeo?a a ii?i?. I?e ?oai?? a?aeaoaa?oueny o?aaeaiiy i??ai?aeieo iae?ii?a o NA1 ?aa?ii? a?iieaiio c iine?aeoth/ith aeoeaaoe??th i?e?iae?? ? ano?ioeeo?a. Iaie aoei iieacaii, ui e?euee?noue LC1+ aeoeaiaaii? i?e?iae?? ??cei ca?eueoo?oueny iaaeiei niie i??ai?aeieo iae?ii?a, a iaio?e i??? ocaeiaae aeaiae?eo?a ? aenii?a a NA1 cii? ia 30-oee aeaiue i?ney ?oai??. Oea neoi/aiiy i?e?iae?? a?ey iae?ii?a ciaoiaeeoueny o?eaaeee ia??iae ? o?eueee ia 90-oo aeiao e?euee?noue i?e?iae?? a oe?e iaeano? iaaeeaea?oueny aei eiio?ieueii? aaee/eie. Iaeiae, ?iciiae?e S100(+ ano?ioeeo?a a CA1 iaeano? a?iieaiio aoa a?aeiinii iiae?aiei aei oaeiai a eiio?ie?, ca aeeeth/aiiyi iieaeoey?iiai oa?o - a i?noe? ?iciiae?eo neiaioe/ieo cae?i/aiue aenii?a i??ai?aeieo iae?ii?a NA3 ciie. Oea i?aeoaa?aeo? aeua ?icaeyiooa oaa?aeaeaiiy i?i o/anoue ae?aeueieo ee?oei a “a?aeaeaeaii?” i?aneiaioe/ieo cae?i/aiue a?ae aeaiae?eo?a o?aaeaieo iae?ii?a ? i?i ?ieue i?e?iae?? a caaacia/aii? oiia, ye? ni?eythoue oaeaeeiio a?aeiiaeaiith o?aaeaieo iae?ii?a i?ney ?oai??. Oe? aeai? ocaiaeaeothoueny c aeniiaeaie Ohno M. (1995) i?i oa, ui i?e?iae?y oaeaeei ?aaao? ia ?oai?th, a oie /an ye a?aeiia?aeue ano?ioeeo?a ? i?iieeiaiiy iae?ioaa?a niinoa??aathoueny ia a?eueo i?ci?o aoaiao /e a?aenooi? cian?i. I?e aeiaaeuei?e ?oai?? aeoeaiaaia i?e?iae?y eieae?coaaeanue a stratum pyramidale CA1 iaeano?, ano?ioeeoe - a ??cieo iaeanoyo a?iieaiio, ye? aeeth/athoue stratum radiatum ? stratum oriens. Aeoeaiaaia i?e?iae?y a CA1 iaeano? a?iieaiio oaeaeoa, i?ae ano?ioeeoe, yeni?ano? ia?eoa?e/i? aaiciae?acaiaiia? ?aoeaioi?e (peripheral benzodiazepine receptors - PBRs), ye? ?iaeoeiaai? i?ney ?oai?? (Stephenson D.T. et al., 1995). Oaeei /eiii, i?e ?oai?? i?e?iae?aeuei? ee?oeie /e i?ie?oa?othoue a?eueo ?ioaineaii, ii??aithth/e c ano?ioeeoaie, /e nooi?iue i?a?aoe?? ?o ?c ?ioeo a?aeae?e?a iiceo cia/ia ii??aithth/e c ano?ioeeoaie. Aoei iieacaii, ui aeoeaaoe?y i?e?iae?? i?e ?oai?? iiina?aaeeiao?oueny i?aeneeaiiyi i?ioean?a oinoi?eethaaiiy oe?iceio a oeeo ee?oeiao (Henrich-Noack P. et al., 1994), nae?aoe??th oaeoi?a TGF -beta 1, yeee ia? iae?ii?ioaeoi?io ae?th ia i??ai?aei? iae?iie (Burne J.F. et al., 1996; Prehn J.H. et al., 1994). LC1 oaeiae aeeiio? caoenio ooieoe?th i?e ?oai??, ?ia?aoth/e ooai?aiiy a?ao?aeiiiai? eeneioe, yea a naith /a?ao, iiaeoeth? ooieoe?th ?aoeaioi??a aeooaiaoo (Eckert W.A. et al., 1994; Rothwell N.J. et al., 1993). I?ioecaiaeueia ae?y LC1 i?aeoaa?aeaeo?oueny oaeiae ?ia?aoaaiiyi IL-1 i?e ?oai?? (Rothwell N.J. et al., 1993). Aeey ?aeaioeo?eaoe?? aeoeaiaaii? i?e?iae?? ie aeei?enoiaoaaee aioeo?ea aei ae?eii?ioa?io LC1, oinoioe?iceio. Karp oa ?io? iieacaee, ui ?O yaey?oueny niaoeeo?/iei ia?ea?ii i?e?iae?? ye a ii?i?, oae ? i?e iaoieia?? (Karp H.L. et al., 1994). Ca iaoeie aeaieie, ii?oieia?/i? oa?aeoa?enoeee LC1+ ? ?O+ aeoeaiaaii? i?e?iae?? aoee iiae?ai? i?ney ia?a??cee i?aei/iiyieiaiai ia?aa. Oa?aeoa? aca?iiae?? i?e?iae?? c iae?iiaie iiaeia i?iaiae?coaaoe, yeui ii??aiyoe ?iciiae?e i?e?iae?? ye a ii?i?, oae ? i?e o?aaeaii?. A ii?i? iae?iie iathoue aeiaa? a?ae?inoee, a i?e?iae?y ?iciiae?ey?oueny ??aiii??ii ii anueiio iiceo, neoi/aiiy oeeo ee?oei i?aeoe/ii a?aenooi?. I?e iaoieia?? ciaioo?oueny aeiaaeeia aeaiae?eo?a, a i?e?iae?y ia?ai?uo?oueny aeeae/a aei o?ea iae?ii?a ? ooai?th? eeanoa?e. Oaeei /eiii, aiae?c iniaeeainoae aeoeaaoe?? i?e?iae?? i?e o?aaeaii? iae?ii?a iiaea neoaeeoe aeoaea /ooeeaei a?ieia?/iei ia?ea?ii (Streit W.J., 1996). Iaie aoei iieacaii, ui aaea /a?ac 4 aeiy i?ney ia?a??cee i?aei/iiyieiaiai ia?aa a i?noe? iaoiaeaeaiiy niie o?aaeaieo iae?ii?a a iaeano? yae?a o??e/anoiai ia?ao noiaao?a iiceo niinoa??aa?oueny neoi/aiiy LC1+ ? ?O+ aeoeaiaaii? i?e?iae??. ?ai?oa aoee aea/ai? ii??aiyeuei? oa?aeoa?enoeee LC1+ ? S100(+ ee?oei i?e ia?a??coe? i?aei/iiyieiaiai ia?ao (Melzer P. et al., 1997) ? aoei iieacaii, ui iaea?eueoa e?euee?noue aeoeaiaaii? i?e?iae?? niinoa??aaeanue i?ioyaii 2-o oeaei?a, ui ni?aiaaea? c ?ioeie aeaieie e?oa?aoo?e. Oae, Eckert W.A. c? ni?aaaoi?aie (1994) aeyaeee neoi/aiiy ?O+ ee?oei a iiia?aeiaiio a?aeae?e? nieiiiai iiceo aaea /a?ac 3 aeiy i?ney ia?a??cee n?aeie/iiai ia?ao, ? aonoioa oeeo ee?oei aoea i?aeaeuaiith iaa?oue /a?ac 3 oeaei? i?ney iia?aoe??. Dusart I. ? Schwab M.E. (1994) aeine?aeaeoaaee aeoeaaoe?th ae?? i?ney eieaeueiiai o?aaeaiiy nieiiiai iiceo. Iaea?eueoa aonoioa i?e?iae?? a o?aaeai?e ae?eyioe? aoea a?aei?/aia /a?ac 4-8 aei?e. I?e oeueiio ia noi?ii? o?aaeaiiy i?ioyaii ia?oiai oeaeiy niinoa??aaeanue eeoa iacia/ia e?euee?noue ?aaeoeaieo ano?ioeeo?a. C?iaeaii i?eiouaiiy, ui i?e?iae?y aeeiio? iae?ii?ioaeoi?io ooieoe?th i?e ia?a??coe? ia?ao. Ca?eueoaiiy e?eueeino? aeoeaiaaieo iae?ioaa?a/i?e?iae?? o nieiiiio iiceo i?ney ia?a??cee aei?caeueieo ei??ioe?a caaacia/oaaei ?aaaia?aoe?th naini?ieo aenii?a, iiaeeeai, caaaeyee aeae?eaiith oeeoie?i?a ? aca?iiae?? c ?ioeie iaiae?iiaeueieie ee?oeiaie a non?aei?o iaeanoyo (Prewitt C.M. et al., 1997). Oaeei /eiii, i?ney ia?a??cee ia?ao a?ey niie o?aaeaieo iae?ii?a niinoa??aa?oueny eieaeueia neoi/aiiy aeoeaiaaieo i?e?iae?aeueieo ee?oei, ye?, iaaooue, aeeiiothoue caoenio ?ieue e ni?eythoue ?aaaia?aoe?? iae?ii?a. Iiaeia i?eionoeoe, ui aeoeaaoe?y i?e?iae?? iia’ycaia c oinoi?eethaaiiyi a?ee?a ca oe?iceiii, ? iiaeeeai, a oeueiio i?ioean? i?eeia? o/anoue oaeiae LC1. Aea? iiaeae?, ia?a??cea ia?ao ? ?oai?y, a?ae??ciythoueny oei, ui a ia?oiio aeiaaeeo i?e iaoai?/iiio o?aaeaii? ii?ooo?oueny eiioaeo aenii?a ? niie; a ae?oaiio aeiaaeeo - i?e ianoa/? eenith aeieea? iaeiie/aiiy NI2 oa i?iaeoeo?a iaoaaie?cio a ee?oeiao. Iiaeia i?eionoeoe, ui aeoeaiaaia i?e?iae?y aeeiio? caoenio ?ieue ? ni?ey? aeaeeaaiith iae?ii?a. A?aeiia?aeue aeoeaiaaii? i?e?iae?? ia o?aaeaiiy ei?e aieiaiiai iiceo, aeeeeeaiiai eaca?iei ii?ii?iaiiyi, caeaaeeoue a?ae nooiaith i?iieeiaiiy eiai a ??ci? oa?e oeaieie. I?e aeiaaeei? oaee? 4 iei ? aia?a?? 22 i?e?Aeae/iae?i?ii a?aeaoaa?oueny aeaiaoo?aoe?y a?ee?a, eiaaoeyoe?y e?i?ae?a ? aeia?iaoaaiiy aiaee a oeaieiao ei?e. Iaie aoei anoaiiaeaii, ui ano?ioeeoe ioi/othoue a iaaaeee?e e?eueeino? ina?aaeie o?aaeaiiy, a oie /an ye LC1 i?e?iae?y ?aaao? aaea /a?ac 3 aei? i?ney o?aaeaiiy ? neoi/o?oueny a?ey ina?aaeea o?aaeaiiy, a /a?ac 6 aei?a ?? e?euee?noue a ei?? cia/ii ca?eueoo?oueny. ?ai?oa aoei iieacaii (Banner L.R. et al., 1997), ui i?e o?aaeaii? ei?e aieiaiiai iiceo ano?ioeeoe ? i?e?iae?y nae?aoothoue eaeeai??-?ia?a?oi?iee oaeoi? (leukemia inhibitory factor (LIF)), yeee ? oaeoi?ii ?inoo, ui ca?eueoo? aeaeeaaiiy iae?ii?a. Oe?eaaee oie oaeo, ui LIF ?ioii?aaeoeai? i?e?iae?y ? ano?ioeeoe ?iciiae?eythoueny a ei?? ia iaai?e a?aenoai? a?ae i?noey o?aaeaiiy, aea ia a?ey naii? ciie o?aaeaiiy. Amat J.A., oa ?io? (1996) iieacaee, ui i?ney o?aaie ei?e o uo??a i?e?iae?y i?ie?oa?o? oaeaeoa ? a a?eueoiio nooiai?, a ii??aiyii? c ?aaeoe??th ano?ioeeo?a. On? ee?oeie iiceo caaaeii aca?iiae?thoue i?ae niaith ca aeiiiiiaith i?aeee?oeieo eiioaeo?a, nae?aoe?? i?ioa?i?a ? a?ieia?/ii aeoeaieo ?a/iaei oa aei?aaeaii? i?e yeeo-iaaoaeue ooieoe?iiaeueieo a?aeoeeeiue. C eiaeiei ?ieii a?aeaoaa?oueny a?eueo aeaoaeueia aea/aiiy ooieoe?iiaeueiiai cia/aiiy ano?ioeeo?a ? i?e?iae?? ia ?aii?o noaae?yo ?icaeoeo iiceo oa c??eiio iiceo a ii?i?, a oaeiae i?e iaoieia?/ieo noaiao. Iaeiei ?c iaoiae?a, ui aeicaiey? aiae?coaaoe i?ioeane ooieoe?thaaiiy iae?ii?a ? oaeoi?e, ye? oi?aaeythoue oeeie i?ioeanaie, ii?oiaaiac ??cieo ee?oei ? ?o ooieoe?iiaeuei? ci?ie a iiceo, ? ?ioiia?noio?i?/iee iaoiae ? noaoenoe/ia ia?iaea. Iiaeaeueo? aeine?aeaeaiiy aoaeooue iai?aaeai? ia aea/aiiy iioiaeaeaiiy ? i?a?aoe?? i?e?iae??, ?? ooieoe?iiaeueiiai cia/aiiy, aecia/aiiy ??cieoe? i?ae i?e?iae??th ? iae?ioaaaie a iioiaaiac? ? o?aaeaiiio iiceo, ?ie? i?ioecaiaeueieo a?ee?a a i?e?iae??, oaeeo ye LC1, ? eiai ca’ycie c oinoi?eethaaiiyi ?ioeo a?ee?a i?e?iae??, aca?iica’ycie i?e?iae?? c ano?ioeeoaie, iae?iiaie oa ?ioeie ee?oeiaie iiceo, ?ie? i?e?iae?? i?e ??cieo o?aaeaiiyo, a oaeiae e?ea?aeaoe?? iaoieia?/ieo i?ioean?a ca aeiiiiiaith aoaeoeaieo e?ea?nueeeo i?aia?ao?a oa ?ioeo iiaeeeaeo ao?o/aiue, ye eaca?ia ii?ii?iaiiy. AENIIAEE Ia iniia? iaea?aeaieo aeaieo iiaeia c?iaeoe ne?aeoth/? aeniiaee: 1.Ii??aiyeueia ioe?iea cae?aiino? ??cieo aioeo?e aeyaeyoue ee?oeie i?e?iae?? aeicaieeea anoaiiaeoe, ui e?iiei?oei-1, a ii??aiyii? c oinoioe?iceiii ? ?cieaeoeiii, aeyaey? iaea?eueoo e?euee?noue i?e?iae?aeueieo ee?oei c iaea?eueo iiaieie ii?oieia?/ieie oa?aeoa?enoeeaie i?e?iae??. Iieacaii, ui e?iiei?oei-1 ? iioeiaeueiei ia?ea?ii aiaai?aeii?, ?icaaeoaeaii? ? aeoeaiaaii? i?e?iae??. 2. A ia??iae c 1 ii 21 aeaiue iinoiaoaeueiiai ?icaeoeo a?aeaoaa?oueny iino?eia o?ainoi?iaoe?y ? aeeoa?aioe?thaaiiy i?e?iae?aeueieo ee?oei ?c aiaai?aeii? a i?ii?aeio, a iio?i a ?icaaeoaeaio i?e?iae?th. 3. A iiaiia?iaeaeaiiio iiceo iaeanoyie ia?aaaaeii? eieae?caoe?? i?e?iae?? ? noaaaio?eeoey?ia ciia eaoa?aeueieo oeoii/e?a. A i?ioean? iinoiaoaeueiiai ?icaeoeo niinoa??aa?oueny i?a?aoe?y i?e?iae?? a ?io? iaeano? iiceo. 4. A c??eiio iiceo aonoioa eieae?caoe?? i?e?iae?? aoea iaioith a ii??aiyii? c ano?ioeeoaie. Iaeano? cina?aaeaeaiiy i?e?iae?? ? ano?ioeeo?a, ye i?aaeei, ia ni?aiaaeaee. 5. I?e aeiaaeuei?e ei?ioei/ani?e ?oai?? iaea?eueo ae?aaeaia ca?eueoaiiy /enaeueiino? LC1+ aeoeaiaaii? i?e?iae?? niinoa??aaeinue a NA1 cii? a?iieaiio. ?aaeoe?y ano?ioeeo?a aoea iacia/iith. 6. I?e ia?a??coe? i?aei/iiyieiaiai ia?ao iaea?eueoa neoi/aiiy LC1+ ? ?O+ aeoeaiaaii? i?e?iae?? niinoa??aaeinue a yae?ao o??e/anoiai ia?ao noiaao?a iiceo ia noi?ii? o?aaeaiiy. 7. I?e eaca?iiio aieea? ia iaeanoue nainiiioi?ii? ei?e aieiaiiai iiceo a ia?o? aei? i?ney o?aaeaiiy cia/aiiy aonoioe LC1+ aeoeaiaaii? i?e?iae?? niinoa??aaeinue a?ey ina?aaeea o?aaeaiiy; aei 6 aeith i?ney iia?aoe?eiiai ia??iaeo - iaeanoue oaeiai neoi/aiiy ?icoe?thaaeanue. ?aaeoe?y ano?ioeeo?a aoea neaaeiae?aaeaiith ? i?iyaeyeanue o?eueee ia 6-e aeaiue. Ia?ae?e iioae?eiaaieo i?aoeue caeiaoaa/a ii oai? aeena?oaoe??. Noaoo?: 1. Naa/aiei A.E., Ieeiiaiei E.?., Neeai A.A. “Eeaoee iee?iaeee iicaa e?unu a iinoiaoaeueiii ia?eiaea (n?aaieoaeueiue eiioiioeeoioeie/aneee aiaeec),” Iae?ioeceieiaey 29, N 3, n.185-193 (1997). 2. Naa/aiei A.E., Ieeiiaiei E.?., Neeai A.A., IaeEaiia Aeae.A. “?ani?aaeaeaiea iee?iaeee e ano?ioeeoia a ?acee/iuo iaeanoyo ii?iaeueiiai c?aeiai aieiaiiai iicaa e?unu,” Iae?ioeceieiaey 29, N 6, n.431-441 (1997). 3. Naa/aiei A.E. “Iee?iaeey a iioiaaiaca e i?e iaoieiaee iicaa,” Iae?ioeceieiaey 30 N 2, n. 136-150 (1998). 4. Ne?ai A.A., I?eiiaiei ?.?., Naa/aiei A.E. “Eoeueoeaiaai? c??ce a?iieaiio - ?icaeoie a oiiaao in vitro oa ii?oieia?/i? ci?ie i?e aenia?eiaioaeuei?e a?iien??,” A?niee A?eioea?e?anueeiai Aea?aeaaiiai Aaa?iiai Oi?aa?neoaoo. Aeione 6, /anoeia1. Ii?oieiaey - i?aeoe/aneay aaoa?eia?ey e iaaeeoeeia. a.Aaeay Oea?eiaue. n. 93-97 (1998). Oace aeiiia?aeae: 1. Naa/aiei A.E. “Aea/aiiy ?aaeoeaieo yeinoae ae?aeueieo ee?oei a?iieaiia i?e aenia?eiaioaeueii aeeeeeai?e ?oai?? iiceo”, O?c?ieia?/iee aeo?iae IAI Oe?a?ie, Iaoa??aee XV C‘?caeo Oe?a?inueeiai O?c?ieia?/iiai Oiaa?enoaa, Oii 44, N 3, n.54 (1998). 2. Ne?ai A.A., I?eiiaiei ?.?., Naa/aiei A.E. “?ioiia?noio?i?/ia aeine?aeaeaiiy i?aaiioeiiai? eoeueoo?e c??c?a a?iieaiia” O?c?ieia?/iee aeo?iae IAI Oe?a?ie, Iaoa??aee XV C‘?caeo Oe?a?inueeiai O?c?ieia?/iiai Oiaa?enoaa, Oii 44, N 3, n.56 (1998). 3. IaeEaiia Aeae.A., Naa/aiei A.E., Ne?ai A.A. ?aaeoe?y i?e?iae?? oa ano?ioeeo?a iiceo ia a?eueii-aeaeo?iiia eaca?ia ii?ii?iaiiy. Oe?a?inueea Oiaa?enoai Iae?iiaoe I (onoaiia/a) Eiioa?aioe?y. 24-26 eenoiiaaea 1998. 4. Savchenko V.L., Nikonenko I.R., McKanna J.A., Skibo G.G.”LC1-positive microglia in normal rat hippocampal slice cultures and after experimental ischemia”; European Meeting on Glial Cell Function in Health and Disease.May 6-10, 1998 Athens, Greece, N122. 5. McKanna J.A., Savchenko V.L., Nikonenko I.R., Skibo G.G. “LC1-positive microglia in the rat brain ontogeny and after transient global ischemia” European Meeting on Glial Cell Function in Health and Disease. May 6-10, 1998 Athens, Greece, N 193. Naa/aiei A.E. I?e?iae?aeuei? ee?oeie iiceo uo?a a iioiaaiac? oa i?e o?aaeaii? ia?aiai? oeaieie.-?oeiien. Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo iaoe ii niaoe?aeueiino? 03.00.13-o?c?ieia?y ethaeeie ? oaa?eie.-?inoeooo o?c?ieia?? ?i.I.I.Aiaiiieueoey IAI Oe?a?ie, Ee?a, 1999. Aeena?oaoe?y i?enay/aia aea/aiith i?e?iae?aeueieo ee?oei, ?o ii?oieia?/ieo ci?i oa i?a?aoe?? i?ioyaii iinoiaoaeueiiai ?icaeoeo iiceo uo??a, ii??aiyiith oiiia?ao?/iiai ?iciiae?eo ?icaaeoaeaii? i?e?iae?? a ??cieo ciiao c??eiai iiceo i?e aeei?enoaii? ??cieo ia?ea??a i?e?iae?? oa ?aaeoe?? i?e?iae?? ia o?aaeaiiy ??cii? aoeieia??. Iieacaii, ui e?iiei?oei-1-iiceoeaia (LC1+) aiaai?aeia i?e?iae?y i?a?o? ?c noaaaio?eeoey?ii? ciie a ??ci? iaeano? iiceo a i?ioean? iioiaaiaco oa aeeoa?aioe?th?oueny a ?icaaeoaeaio oi?io. Ii??aiyeueiee aiae?c iieacaa, ui ii?oieia?/i? oa?aeoa?enoeee LC1+ ?icaaeoaeaii? i?e?iae?? aoee iiae?aieie aei oinoioe?icei-iiceoeaii? oa ?cieaeoei-iiceoeaii? i?e?iae??, aea LC1+ aeyaey? iaea?eueoo e?euee?noue i?e?iae?aeueieo ee?oei c iaea?eueo iiaieie ii?oieia?/ieie oa?aeoa?enoeeaie i?e?iae??. A c??eiio iiceo aonoioa eieae?caoe?? i?e?iae?? aoea iaiueoith, ii??aithth/e c ano?ioeeoaie. Iaeano? cina?aaeaeaiiy i?e?iae?? oa ano?ioeeo?a ia ni?aiaaeaee. I?e aeiaaeuei?e ei?ioei/ani?e ?oai?? LC1+ aeoeaiaaia i?e?iae?y neoi/oaaeanue a a?eueo?e e?eueeino? a NA1 iaeano? a?iieaiio. A?aeiia?aeue LC1+ oa oinoioe?icei-iiceoeaii? aeoeaiaaii? i?e?iae?? niinoa??aaeanue a yae?ao o??e/anoiai ia?ao noiaao?a iiceo ia ia?eoa?e/ia o?aaeaiiy iaei??? ?c eiai ?icaaeoaeaiue. A ia?o? aei? i?ney eaca?iiai aieeao ia iaeanoue nainiiioi?ii? ei?e aieiaiiai iiceo LC1+ i?e?iae?y ?aaao? oaeaeoa ? ?ioaineai?oa, a ii??aiyii? c ano?ioeeoaie. Ia iniia? iaea?aeaieo ?acoeueoao?a iiaeia caeeth/eoe, ui LC1+ ? iioeiaeueiei ia?ea?ii i?e?iae?? a iioiaaiac?, c??eiio iiceo oa i?e o?aaeaiiyo ??cii? aoeieia??. Iiaeeeai, ui LC1+ i?eeia? aeoeaio o/anoue a ci?i? noaio i?e?iae??, oinoi?eethaaii? a?ee?a ca oe?iceiii ? ?aaaia?aoe?? iae?ii?a i?ney o?aaeaiiy iiceo. Naa/aiei A.E. Iee?iaeeaeueiua eeaoee iicaa e?unu a iioiaaiaca e i?e ii?aaeaiee ia?aiie oeaie.-?oeiienue. Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.13-oeceieiaey /aeiaaea e aeeaioiiai.-Einoeooo oeceieiaee ei.A.A.Aiaiiieueoea IAI Oe?aeiu, Eeaa, 1999. Aeenna?oaoeey iinayuaia eco/aieth iee?iaeeaeueiuo eeaoie, eo ii?oieiae/aneeo eciaiaiee e iea?aoeee a oa/aiea iinoiaoaeueiiai ?acaeoey iicaa e?un, n?aaiaieth oiiia?aoe/aneiai ?ani?aaeaeaiey ?acaaoaeaiiie iee?iaeee a ?acee/iuo iaeanoyo c?aeiai iicaa n eniieueciaaieai ?acee/iuo ia?ea?ia iee?iaeee e ?aaeoeee iee?iaeee ia ii?aaeaiey ?acee/iie yoeieiaee. Iieacaii, /oi eeiiei?oei-1-iieiaeeoaeueiay (LC1+) aiaaieaeiay iee?iaeey iea?e?oao ec noaaaio?eeoey?iie ciiu a ?acee/iua iaeanoe iicaa a i?ioeanna iioiaaiaca e aeeooa?aioee?oaony a ?acaaoaeaiioth oi?io. N?aaieoaeueiue aiaeec iieacae, /oi ii?oieiae/aneea oa?aeoa?enoeee LC1+ ?acaaoaeaiiie iee?iaeee auee noiaeiuie n oinoioe?icei-iieiaeeoaeueiie e ecieaeoei-iieiaeeoaeueiie iee?iaeeae, iaeiaei LC1+ auyaeyao iaeaieueoaa eiee/anoai iee?iaeeaeueiuo eeaoie n iaeaieaa iieiuie ii?oieiae/aneeie oa?aeoa?enoeeaie iee?iaeee. A c?aeii iicaa ieioiinoue eieaeecaoeee iee?iaeee auea iaiueoae ii n?aaiaieth n ano?ioeeoaie. Iaeanoe nin?aaeioi/aiey iee?iaeee e ano?ioeeoia ia niaiaaeaee. I?e aeiaaeueiie e?aoeia?aiaiiie eoaiee LC1+ aeoeae?iaaiiay iee?iaeey neaieeaaeanue a aieueoii eiee/anoaa a NA1 iaeanoe aeiiieaiia. Ioaao LC1+ e oinoioe?icei-iieiaeeoaeueiie aeoeae?iaaiiie iee?iaeee iaaethaeaeny a yae?ao o?ieie/iiai ia?aa noaiea iicaa ia ia?eoa?e/aneia iia?aaeaeaiea iaeiie ec aai aaoaae. A ia?aua aeie iinea eaca?iiai aicaeaenoaey ia iaeanoue nainiiioi?iie ei?u aieiaiiai iicaa LC1+ iee?iaeey ?aaae?oao auno?aa e eioaineaiaa ii n?aaiaieth n ano?ioeeoaie. Ia iniiaa iieo/aiiuo ?acoeueoaoia iiaeii caeeth/eoue, /oi LC1+ yaeyaony iioeiaeueiui ia?ea?ii iee?iaeee a ?acaeaathuainy, c?aeii iicaa e i?e ii?aaeaieyo ?acee/iie i?e?iaeu. Iiaeii i?aaeiieiaeeoue, /oi LC1+ i?eieiaao aeoeaiia o/anoea a eciaiaiee ninoiyiey iee?iaeee, oinoi?eee?iaaiee aaeeia ii oe?iceio e ?aaaia?aoeee iae?iiia iinea ii?aaeaiee iicaa. Eeth/aaua neiaa: eeiiei?oei-1, oinoioe?icei, ecieaeoei, S100(-aeeeii?ioaei, ?acaaoaeaiiay iieiyuayny e aeoeae?iaaiiay iee?iaeey, ano?ioeeou. I?aeienaii aei ae?oeo 06.08.98 ?. Oi?iao 60o90/16. Oi. Ae?oe. A?e.2,0. Iae.-aeae.a?e.2,0. Iaeeaae 100.Cai.220. Aoe. *a?aiiia?i?enueea, 57/3, e.101. A?aeae?e iia?aoeaii? iie?a?ao?? Oeaio?o I?aeia?iaeii? ina?oe 227-12-75, 227-37-86 Oaaeeoey. E?euee?noue i?e?iae?aeueieo ee?oei oa ano?ioeeo?a ia iaeeieoeth ieiu? (ii2) a ??cieo ?aa?iiao c??eiai ii?iaeueiiai iiceo uo??a. Aonoioa (ee?oei/ii2) ?aa?iie iiceo LC1 PT GSA S100( NA?AAeI? CIA*AIIss 103±3 54±2* 33±1* 217±10* IA?AAeI?E IICIE 115±2 61±2* 32±3* 184±1* EI?A 104±5 58±4* 34±2* 168±24* eiaia 123±10 63±5* - 143±15 iiynia 116±8 36±6* - 156±5* o?i’yia 116±1 62±1* 36±1* 135±4* iioeee/ia 98±8 54±3* 25±5* 208±34* A?IIEAII 94±9 55±2* 27±4* 267±26* ciia CA1 91±3 - - 244±12* iaae?aeueia (u?eeia) 109±18 - - 232±51 coa/anoa ai?icia 90±9 - - 257±14* ciia CA2 84±9 - - 226±11* aieieieno? ae?eyiee 88±37 - - 226±45 IAINO??AOOI 130±5 45±5* - 198±11* aacaeueiee 123±8 - - 186±5* ai?eaeueiee 138±8 35±4* - 164±9 eaoa?aeueiee 123±2 32±4* - 172±12* iaae?aeueiee 131±13 68±5* - 230±19* A?EA ?A*IAEIA 105±8 - - 279±46* OAEAION 96±3 43±2* 47±3* 240±29* A?IIOAEAION 121±1 62±6* 38±2* 265±8* NA?AAeI?E IICIE 90±2 54±6* 34±4* 176±4* CAAeI?E IICIE 51±4 48±4 - 174±5* * - ?<0.05 i?e ii??aiyii? a?oie LC1-iiceoeaieo ee?oei c a?oiaie ?O-, GSA- ? S100(-iiceoeaieo ee?oei. I?ei?oea: LC1 - e?iiei?oei-1, ?O- oinoioe?icei, GSA- ?cieaeoei oa S100(-a?eie.

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