I?I?NOA?NOAI IOI?IIE CAeI?IA’ss OE?A?IE

IAOe?IIAEUeIEE IAAeE*IEE OI?AA?NEOAO

?I.I.I.AIAIIIEUeOess

Ei?ithoaiei Ieueaa Ieeiea?aia

OAeE 616.988.23-092.4-092:612.014.1:615.371:578.53:575.857:578.835.15

?ioiiiaoiaaiac aenia?eiaioaeueiiai iie?ii??e?oo c iiceoe?e
aaoa?iaaiiino? iiioeyoe?e iie?ia??on?a

03.00.06 — a??onieia?y

A A O I ? A O A ? A O

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy aeieoi?a a?ieia?/ieo iaoe

Ee?a- 1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia a Iaoe?iiaeueiiio iaaee/iiio oi?aa?neoao?

?i.I.I.Aiaiiieueoey IIC Oe?a?ie

Iaoeiaee eiinoeueoaio: aeieoi? iaaee/ieo iaoe,
i?ioani?,

caneoaeaiee ae?y/ iaoee ? oaoi?ee Oe?a?ie,

/eai-ei?aniiiaeaio IAI oa AII Oe?a?ie

Oe?iaieia Aieiaeeie? Iaaeiae/, caa?aeoaa/ eaoaae?ith i?e?ia?ieia??,
a??onieia?? oa ?ioiieia?? Iaoe?iiaeueiiai iaaee/iiai Oi?aa?neoaoo
?i.I.I.Aiaiiieueoey

Io?oe?ei? iiiiaioe:

aeieoi? a?ieia?/ieo iaoe, i?ioani? Ni?aae Ieeiea sseiae/,

caa?aeoaa/ a?aeae?eii i?iaeai ?ioa?oa?iio oa ?ioiiiiaeoeyoi??a

?inoeoooo i?e?ia?ieia?? oa a??onieia??

?i. Ae. E. Caaieioiiai IAI Oe?a?ie

aeieoi? iaaee/ieo iaoe, i?ioani? *oaeia Ethaeieea Ieo?ioai?aia,

i?ia?aeiee iaoeiaee ni?a?ia?oiee eaai?aoi??? ea?iaaieo ?ioaeoe?e

oa cania?a ?o ?ioiii?io?eaeoeee

Ee?anueeiai iaoeiai-aeine?aeiiai ?inoeoooo ai?aeai?ieia?? oa
?ioaeoe?eieo

caoai?thaaiue ?i. E. A. A?iiaoaanueeiai IIC Oe?a?ie

aeieoi? iaaee/ieo iaoe, i?ioani? Aeiia?aae ?aai Aiae??eiae/

caa?aeoaa/ eaai?aoi???th o?aini?neaieo a??on?a

Euea?anueeiai iaoeiai-aeine?aeiiai ?inoeoooo ai?aeai?ieia??

oa a?a??ie IIC Oe?a?ie

I?ia?aeia onoaiiaa : Ee?anueea iaaee/ia aeaaeai?y i?neyaeeieiiii? ina?oe
?i. I.E.Ooieea

Caoeno a?aeaoaeaoueny “ 20 ” o?aaiy 1999 ?. i 13.30 aiaeei? ia
can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee

Ae 26.003.01 Iaoe?iiaeueiiai iaaee/iiai Oi?aa?neoaoo ?i. I.I.
Aiaiiieueoey (252 057, i. Ee?a, i?ini. Ia?aiiae 34, iaae.-i?io. ei?ion,
aoae. ?2)

C aeena?oaoe??th iiaeia iciaeiieoenue a a?ae?ioaoe? Iaoe?iiaeueiiai
iaaee/iiai oi?aa?neoaoo ?i.I.I.Aiaiiieueoey (aoe. Ciieia?/ia 3)

Aaoi?aoa?ao ?ic?neaiee “ 19 ” ea?oiy 1999 ?.

A/aiee nae?aoa? niaoe?ae?ciaaii? a/aii? ?aaee

aeieoi? iaaee/ieo iaoe, i?ioani?
I. I. ssai?ianueeee CAAAEUeIA
OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue ?iaioe. ?IE – aaiiii? aioa?ia??one a?ae??ciythoueny a?ae
a??on?a ?ioeo oaenii?a oe?ieei ?iciianthaeaeaiiyi, oee?eoeyoe??th a
i?aai?ci? ethaeeie oa ioi/oth/iio na?aaeiaeu? (?.A.Aii/a?oe o ni?aaao.,
1990; A.I.Ae?ei o ni?aaao., 1993; A.?.Caaei?iaeia o ni?aaao., 1997),
iie?i?aaiiei o?ii?ciii oa a?aeiia?aeii ??ciiiai?oiei cia/aiiyi a
?ioaeoe?ei?e oa ia?ioaeoe?ei?e iaoieia?? ethaeeie (M.Voroshilova, 1989;
H.A.Rotbart et al., 1995; A.I.Oe?iaieia o ni?aaao., 1998), cia/iith
i?ieea?noth iiioeyoe?e (D.H.Bishop, 1988; A.I.Eeaa?aeei a niaao., 1991;
E.Wimmer et al., 1993; Y.Ghendon et al., 1994). I?ney a?aee?eooy ?o
oeiiaeo i?aaenoaaiee?a – iie?ia??on?a, ?ioiiiaoiaaiac iie?ii??e?oii?
?ioaeoe?? aea/aee ?ioaineaii oa ??ciiieaiiai (A.A.Ei?ieaaa a niaao.,
1975; E.A.Ooeaeeia, 1975; E.A.?iaeicii a niaao., 1978; S.Tracy et al.,
1991; R.Welliver et al., 1993). Noai?aiiy a 50-60 ?iee Naa?iei oa Nieeii
aeeai? (i?aeueii? IIA — aai AEIA) oa ?iaeoeaiaaii? aaeoeeie (?IA) oa
aecia/i? oni?oe a i?io?eaeoeoe? iie?ii??e?oo (J.Salk, 1984; A.Sabin,
1985, H.Faden, 1993) ia?aae/anii ciaioeee oaaao aei aenia?eiaioaeueiiai
aea/aiiy iie?ia??onii? ?ioaeoe??. ?aea? aioa?ia??onieia??,
?ioiiiaoieia??, iiioeyoe?eii? aaiaoeee a??on?a, ye? ii/aee ?ic?iaeyoeny
a 80-90 ?iee iinoaaeee aaaaoi iiaeo ieoaiue, ui iio?aaoaaee
i?iaeiaaeaiiy aea/aiiy iie?ia??on?a, iniaeeainoae ?ioi?oaoo oa
iaoiaaiaco iie?ii??e?oo.

Ii-ia?oa, ?nio? iino?eia ?aaeueia caa?ica aeieeiaiiy aiane?aeie
iooaoe?e, ?aeiia?iaoe?e iiaeo aa??aio?a aioa?ia??on?a ?c ci?iaiith
a??oeaioi?noth, aioeaaii?noth, i?e iie?ia??oni?e ?ioaeoe?? oa
aaeoeeiaeueiiio i?ioean?, i?e ianaaeao a eaai?aoi?ieo oiiaao aai i?ae
/an oee?eoeyoe?? iie?ia??on?a a ioi/oth/iio na?aaeiaeu?, (R.Crainic et
al., 1984; M.Georgescu et al., 1995; F.Friedrish et al., 1995; R.Tafes
et al., 1997). Iiaeeaeaia aea/aiiy iaoai?ci?a aaiethoe?? aioa?ia??on?a c
iiceoe?e aeinyaiaiue iiioeyoe?eii? aaiaoeee a??on?a aoei i?aea?oioyi
aeey a?aee?eooy yaeua aeenioe?aoe?? iiioeyoe?e a??on?a Eienae? A a
i?ioean? ?o ?ai?iaeoeoe?? ia aaiaoe/i? aa??aioe c ??cieie
o?ceei-o?i?/ieie oa a?ieia?/ieie aeanoeainoyie (A.I.Oe?iaieia, 1977).
Aeenioe?aoe?y aioa?ia??on?a ? iaeiei c oaeoi??a, ui i?ecaiaeeoue aei
aaoa?iaaiiino? ?o iiioeyoe?e. Aa??aioe iiioeyoe?e aioa?ia??on?a
ii-??ciiio aieeaathoue ia iaoiaaiac ?ioaeoe??, oi?ioaaiiy ?ioi?oaoo,
iathoue ??ci? ia?ea?e aeey ?aeaioeo?eaoe?? (A.I.Oe?iaieia o ni?aaao.,
1996; 1998) Iniaeeaiai i?aeoe/iiai cia/aiiy oaiiiai aeenioe?aoe??
aioa?ia??on?a iiaea iaoe aeey oaeineiiaeaiiy aaeoeeiieo ooai?a a??on?a
iie?ii??e?oo oeyoii naeaeoe?? c ?o iiioeyoe?e aeenioe?aio?a c aenieith
aioeaaiiith oa ?ioiiaaiiith ae??th (I.A.Oeeia o ni?aaao., 1997;
I.I.Ei?ithoaiei o ni?aaao., 1997).

Ii-ae?oaa, aeieeiaiiy nai???aeiiai iai?yieo a ?ioiiiaoieia?? – a/aiiy
i?i ?ioiiiiaeoethth/? aeanoeaino? a??on?a (M.Bendinelli, 1988;
A.O.O?ieia a niaao., 1985; A.O.Naiaiia, 1989; I.I.Ei?ithoaiei o
ni?aaao., 1997), ? i?aenoaaith aeey aea/aiiy a oaeiio aniaeo?
aioa?ia??on?a. Aoaioeiaai? ooaie a??on?a iie?ii??e?oo na?aae ?ioeo
aioa?ia??on?a iaea?eueoa i?aaenoaaeai? a ioi/oth/iio na?aaeiaeu? oa
i?aai?ci? ethaeeie caaaeyee iania?e ?ioi?caoe?? IIA. ?o iaeaea iino?eiee
aieea ia ?ioiio nenoaio, ia a?aeiia?aeue aei aaoa?ieia?/ieo aioeaai?a, a
oiio /ene? aei ?ioeo aaeoeei, noaaeoue ieoaiiy i?i ?ioiiiiaeoethth/?
aoaeoe, ui aeieeathoue ye iane?aeie (I.I.Ei?ithoaiei, 1998).

Ii-o?ao?, iaaaeuei? iio?aae oaeineiiaeaiiy iie?ii??e?oieo aaeoeei,
iniaeeai IIA, a oaeiae noai aaeoeeiaoe??, uaieaiiy ?ioiiaeao?oeeoieo
in?a, anioe?aoe?? aaeoeei IIA, ?IA c ?ioeie, iai?eeeaae c anioe?eiaaiith
eaoetheiai-aeeooa??eii-i?aaoeaaith aaeoeeiith – AEAeI, iiaeeeaeo
niieo/aiue c e?ea?nueeeie i?aia?aoaie (J.Grabstein, 1990; WHO Drug Int.,
1995; R.Vetter et al., 1997; H.Wyatt, 1997) iio?aao? aeine?aeaeaiiy
?ioiiiiaeoethth/i? ae?? aoaioeiaaieo ooai?a iie?ia??on?a ia ??ai? ?o
iiioeyoe?e oa aaeoeei a oe?eiio.

Anaiaeay AIIC i?eeiyea ?aciethoe?th i?i e?ea?aeaoe?th iie?ii??e?oo a
na?o? aei 2000 ?ieo, caaaeath/e ia ai?aeai?/i?noue ?ioaeoe??, aaeeea
nioe?aeueia cia/aiiy ?iaae?ae?caoe?? aiane?aeie ia?aianaiiy iie?ii??e?oo
oa iiaeeeaino? ea?iaaiiai aieeao ia oeae i?ioean aaeoeeiaie IIA oa ?IA
(Expanded Programme on Immunization, WHO, 1997; Vaccine Weekly, 1997;
J.Nossal, 1997). C 1995 ?ieo i?ae aa?aeith AIIC a na?o? i?iaiaeyoueny
aei? Iaoe?iiaeueii? ianiai? ?ioi?caoe?? ae?oae ia?oeo ?ie?a aeeooy a
ai?aeai?/ieo ii iie?ii??e?oo e?a?iao. Oe?a?ia i?aeeeth/eeany aei oe???
i?ia?aie a 1996 ?ioe?, aeecueei 2 iei. ae?oae a?ae 3-o i?nyoe?a aei 3-o
?ie?a iaea?aeaee ii aea? aeice IIA.A na?o? o?eaa? aeeneon?y i?i ia?aaaae
oa iaaeie?ee IIA-?IA (J.Modlin, 1995; D.Henderson et al., 1995; D.Greco
et al., 1997).Aeaye? aeine?aeieee a?aeaeathoue ia?aaaao aeey ??oaiiy
i?iaeaie e?ea?aeaoe?? iie?ii??e?oo ?IA, oiio ui oey aaeoeeia ia? a?eueo
aenieo aioeaaiio ae?th i?ae IIA, ia?aoeiaeaea? aeieeiaiith
aaeoeeiianioe?eiaaiiai ia?ae?oe/iiai iie?ii??e?oo (AAII), ia
i?ecaiaeeoue aei oee?eoeyoe?? iie?ia??on?a.(P.Ogra, 1994; S.Plotkin,
1997). A?aeiinii IIA aieiaieie ia?aaaaaie aaaaeathoue eaaeee niin?a
aaaaeaiiy, iaioo aa?o?noue, ooai?aiiy caaaeueiiai oa eieaeueiiai
?ioi?oaoo. Iaaeie?eaie ? ia aeinoaoiuei aenieee ??aaiue ?ioi?oaoo,
iiaeeeaa ?ioa?oa?aioe?y i?ae iie?ia??onaie ??cieo oei?a, aeieeiaiiy AAII
(J.Melnik, 1993; M.Georgescu et al., 1994; F.Friedrich et al., 1995),
oae? aeiaaeee iae/ano?oa iia”ycai? c iie?ia??onaie ??, ??? oei?a. A
na?aaeiueiio iieaciee caoai?thaaiue ia AAII neeaaea? 1 ia 1 iei. 200
oen. aeic aaeoeeie (M.Georgescu et al., 1997). A Oe?a?i? a inoaii? ?iee
oaeiae niinoa??aathoueny aeiaaeee AAII (E.I.O??oeiaa o ni?aaao., 1995) o
aaeoeeiiaaieo IIA ae?oae, iniaeeai aaeoeeiiaaieo ?c cai?ciaiiyi, aai o
eiioaeoieo in?a. A i?aeoeoe? ia ia? naino i?ioenoaaeyoe iaeaeaa oeie
aaeoeei, no?aoaa?y aaeoeeiaoe?? iiaeiia aooe aio/eith, a caeaaeiino?
a?ae ai?aeai?ieia?/ii? neooaoe??, eiioeiaaioo, ?aa?iio. Caaaeath/e ia
iacaaeia?eueiee eieaeoeaiee ?ioi?oao aei iie?ii??e?oo, ai?aeai?/io
neooaoe?th a Oe?a?i? , aoee i?iaaaeai? aeine?aeaeaiiy ii aeei?enoaiith
?IA aeey aaeoeeiaoe?? ae?oae, c ianooiiith ?aaaeoeeiaoe??th IIA
(A.E.Caaei?iaeiay a niaao., 1997). Iieacaii, ui i?ney ?aaaeoeeiaoe?? an?
ae?oe iaee aeu? aioeo?ea aei iie?ia??on?a, ui na?ae/eoue i?i
aeioe?euei?noue aeei?enoaiiy ?IA a Oe?a?i?. Ca i?eeiyoei a Oe?a?i? oa
aeayeeo ?ioeo e?a?iao eaeaiaea?ai uaieaiue iaeii/anii c aaaaeaiiyi IIA
i?iaiaeeoueny aaeoeeiaoe?y ae?oae AEAeI. I?neyaaeoeeiaeuei? oneeaaeiaiiy
a oaeeo aeiaaeeao iiaeooue aooe iia”ycai? ?c aca?iiith ?ioiiiiaethth/ith
ae??th iaio aaeoeei, aioeaaiiei ia?aiaaaioaaeaiiyi i?aai?cio, ooai?aiiyi
ooeiaeaeoth/eo eiiiiiaio?a (Y.A.Ao?ae/ a niaao., 1991; A.A.Aei?ioae/oe a
niaao., 1991; I.I.Ei?ithoaiei o ni?aaao., 1997). Aca?iiae?y aaeoeei i?ae
niaith ? ua aeaeaei ia ae??oaiei ieoaiiyi aeey oai??? oa i?aeoeee. Aeey
eiia?iiaaiiai canoinoaaiiy aaeoeei, iai?eeeaae AEIA oa AEAeI, aai ?IA c
AEAeI iaiao?aeii ia?aeiiaiiy a a?aenooiino? ?ioa?oa?aioe?? i?ae
aeeooa??eiei , i?aaoeaaei oieni?aeaie a neeaae? DTP- AEAeI oa
iie?ia??onaie. Iae/ano?oa a na?o? aeey caiia?aaiiy iaa?ieia?/ieo
oneeaaeiaiue, aioeaoaeiiao?e, iaoiiaeaieo AEAeI (DTP), a oaeiae
i?iaieaoe?? ?icaeoeo ia?ae?oe/iiai iie?ii??e?oo o ae?oae canoiniaothoue
?IA (D.Miller et al., 1981; R.Lagos et al., 1988; H.Wyatt, 1997). A
oiiaao no/anii? ae?eniino? a Oe?a?i? ia oii? iioe?aieo ai?ciae?a
?ioiiaeao?oeeoo o ianaeaiiy i?iaaaeaiiy iaeii/anii? ?ioi?caoe?? ae?oae
3-o – 5-oe i?ny/iiai a?eo aai aei?ineeo aaeoeeiaie AEIA oa AEAeI noa?
aaaeeeaith i?iaeaiith(A.E.Aiiaea?aiei a niaao., 1991; 1998; E.I.*oaeia o
ni?aaao.,1996). A?aaeeeaoe?y iie?ii??e?oo a Oe?a?i?, aoaeoeaia
?ioi?caoe?y ianaeaiiy, aeineiiaea ae?aiinoeea iie?ia??onii? ?ioaeoe??
aeiaaathoue a?aeiia?aeiiai oai?aoe/iiai iaa?oiooaaiiy c o?aooaaiiyi
i?ioean?a aeenioe?aoe??, yea a?aeaoaa?oueny i?ae /an ?ai?iaeoeoe??
aioa?ia??on?a (A.I.Oe?iaieia, 1977; 1989; 1996). Aeua iaaaaeaia iaaea?
aeooaeueiino? i?iaeai? aeeaieiai aea/aiiy ?ioiiiaoiaaiaco
aenia?eiaioaeueiiai iie?ii??e?oo c iiceoe?e aaoa?iaaiiino? iiioeyoe?e
iie?ia??on?a oa a?ieia?/ieo aeanoeainoae ?o aa??aio?a-aeenioe?aio?a.
Oeei aecia/athoueny aeooaeuei?noue, iaoa oa caaaeaiiy aeaii? ?iaioe.

Ca”ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie

?acoeueoaoe aeine?aeaeaiue (1981-1998 ?ie?a) cae?eniaieo ca i?ia?aiaie
IIC Oe?a?ie, Aea?aeaaiiai eii?oaoo c iaoee oa oaoi?ee, Aea?aeaaiiai
eii?oaoo ai?ioueae ?c NI?Aeii, aa?eoee aei oai: “Iiioeyoe?eia
i?ieea?noue aioa?ia??on?a, ?? cia/aiiy a iaoieia?? oa i?io?eaeoeoe?
aioa?ia??onieo ?ioaeoe?e” ? aea?aea?no?aoe?? 0193V19803; “Iniaeeaino?
?ioiiiaoiaaiaco iie?ii??e?oo i?e aaeoa??aeueii-a??onieo anioe?aoe?yo” ?
aea?aea?no?aoe?? 0193 V019817; ”Aieea aeeai? iie?ii??e?oii? aaeoeeie ia
?ioiio nenoaio” ? aea?aea?no?aoe?? 0194V025378;”Iaea?aeaiiy
iiiieeiiaeueieo aioeo?e aeey aeeoa?aioe?aoe?? aaeoeeiieo oa a??oeaioieo
a??on?a iie?ii??e?oo” ? aea?aea?no?aoe??0193V019812; ”?ioa?oa?aioe?y
i?ae aioa?ia??onaie oa ?ao?ia??onaie ye iiaeeeaee oeyo i?io?eaeoeee
NI?Aeo oa NI?Ae-anioe?eiaaieo ?ioaeoe?e”? aea?aea?no?aoe??
0195V019537;”Oaeineiiaeaiiy iaoiaeieia?? aaeoeeiaoe?? oa eaeaiaea?th
uaieaiue i?ioe iie?ii??e?oo, eaoetheo, aeeooa???, i?aaoeth. Noai?aiiy
oeyoii e??ieiina?aoaaiiy iocath eoeueoo? ee?oei, a?a?eaeii, a??on?a aeey
i?io?eaeoeee iie?ii??e?oo” ? aea?aea?no?aoe??0195V014159.

Iaoa ?iaioe: Ia iniia? aeine?aeaeaiiy ?ioiiiaoiaaiaco
aenia?eiaioaeueiiai iie?ii??e?oo oa a?ieia?/ieo aeanoeainoae
iie?ia??on?a ia ??ai? a??onieo iiioeyoe?e iaa?oiooaaoe oeyoe
oaeineiiaeaiiy ?ioiii?io?eaeoeee iie?ii??e?oo.

Caaaeaiiy aeine?aeaeaiiy:

I?iaanoe aea/aiiy aaoa?iaaiiino? iiioeyoe?e aaeoeeiieo oa a??oeaioieo
a??on?a iie?ii??e?oo ia i?eeeaae? aeenioe?aoe??. Ii??aiyoe iniiai?
a?ieia?/i? aeanoeaino? – a??oeaioi?noue, aioeaaii?noue, ?ioiiaaii?noue
aeenioe?aio?a iie?ia??on?a.

Aeine?aeeoe ?ioiiiaoiaaiaoe/i? iniaeeaino? aenia?eiaioaeueiiai
iie?ii??e?oo c o?aooaaiiyi aeenioe?aoe?? iie?ia??on?a.

Ioe?ieoe ?ioiiiiaeoethth/? aeanoeaino? aaiaoe/ieo aa??aio?a iiioeyoe?e
iie?ia??on?a, ?ice?eoe iiaeeea? iaoai?cie ?o ?ioiiiiaeoethth/i? ae??.

Aeyaeoe oa ioa?aeoa?ecoaaoe ci?ie oeueo?ano?oeoo?ii? i?aai?caoe??
?ioiieiiiaoaioieo i?aai?a, iaoiiaeai? ?ioiiiiaeoethth/ith ae??th
aaiaoe/ieo aa??aio?a iie?ia??on?a aai iie?ii??e?oieo aaeoeei.

Aea/eoe aca?iio ?ioiiiiaeoethth/o ae?th aaeoeei AEIA oa AEAeI,
aenia?eiaioaeueii iaa?oiooaaoe oaeineiiaeaiiy noaie ?ioi?caoe?? i?ioe
iie?ii??e?oo, aeeooa???, i?aaoeth.

Ioe?ieoe ia iaiaaeaiiio eiioeiaaio? ae?oae aoaeoeai?noue ciaioaiiy
aca?iii? ?ioiinoi?an?? aaeoeei AEIA oa AEAeI oeyoii ?icae?eueii?
?ioi?caoe??.

Iaoeiaa iiaecia io?eiaieo ?acoeueoao?a. ?iaioa a?aeiineoueny aei
oai?aoe/ii? a??onieia??, a i?e ae?noaee i?iaeiaaeaiiy ?aea? i?ioani?a
A.I. Oe?iaieiaa, yeee a?aee?ea oaiiiai aeenioe?aoe?? iiioeyoe?e
aioa?ia??on?a(A.I.Oe?iaieia,1977,1996), ia iiaeae? Eienae? A a??on?a
ioa?aeoa?ecoaaa caeiiii??iino? yaeua aeenioe?aoe?? oa aeanoeaino?
aeenioe?aio?a. I?ae eiai ea??aieoeoaii iaoeiaoe? ?icaeaaee a
aeena?oaoe?eieo ?iaioao ??ci? aniaeoe a/aiiy i?i aeenioe?aoe?th, a oeeo
aeine?aeaeaiiyo ia aoee aea/ai? ieoaiiy aaoa?iaaiiino? iiioeyoe?e
iie?ia??on?a, a?ieia?/i? oa?aeoa?enoeee aeenioe?aio?a, iniaeeai
aaeoeeiieo ooai?a, ?ioiiiaoiaaiac aenia?eiaioaeueiiai iie?ii??e?oo c
iiceoe?e aaoa?iaaiiino? ?o iiioeyoe?e. A??one iie?ii??e?oo ye
?ioiiiiaeoeyoi?e aoee aia?oa aea/ai? aaoi?ii ia eaoaae?? i?e?ia?ieia??,
a??onieia?? oa ?ioiieia?? IIO, iaoeia? ?aea? oeeo aeine?aeaeaiue ?
aeanieie, ia?o? ioae?eaoe?? ia?ii c 1983 ?ieo. A iao?e ?iaio? aia?oa
aoei i?iaaaeaii aeeaiea ii??aiyeueia aea/aiiy aaaeeeaeo aeey
?ioiiiaoiaaiaco a?ieia?/ieo oa?aeoa?enoee aaeoeeiieo oa a??oeaioieo
ooai?a a??on?a iie?ii??e?oo ia ??ai? iiioeyoe??:

Aoei anoaiiaeaii, ui aaiaoe/iee aa??aio – aeenioe?aio Aaaio+, yeee
caeiiii??ii aeae?ey?oueny i?e ?ai?iaeoeoe?? ye aaeoeeiieo, oae ?
a??oeaioieo a??on?a iie?ii??e?oo, iaoiiaeth? iaea?eueoee i?iya
ea?aeeiaeueieo a?ieia?/ieo aeanoeainoae: a??oeaioiino?, aioeaaiiino? oa
?ioiiaaiiino?.

Aeyaeaii, ui a??one iie?ii??e?oo iathoue ae?aci? ?ioiiiiaeoethth/?
aeanoeaino?, i?eoaiaii? ye a??oeaioiei, oae ? aaeoeeiiei ooaiai. Ia
??ai? iiioeyoe?e ?ioiinoi?an?y a cia/i?e i??? iia’ycaia c aaiaoe/iei
aa??aioii Aaaio(.

Aia?oa ia iiaeae? ieoae aeyaeaii, ui aaeoeeii? ooaie a??on?a
iie?ii??e?oo iathoue caeaoi?noue oaeaeei i?iieeaoe a i?aaie ?ioiii?
nenoaie oa ?iciiiaeoaaoeny a ieo, oeth aeanoea?noue aoei iacaaii
?ioiii?aaiio?ii?ciii. Aaiaoe/iee aa??aio Aaaio( ?iaeoeo? aiiioic a
e?ioioeeoao naeac?iee.

Iieacaii,ui aeeaa iie?ii??e?oia aaeoeeia, ia a?aei?io a?ae
?iaeoeaiaaii?, ni?e/eiy? noi?an?th ?ioiii? a?aeiia?ae? aei aeeooa??eiiai
oa i?aaoeaaiai aiaoienei?a. Aaeoeeia AEAeI ?ioaineaii i?eai?/o?
aoii?aeueiee oa ee?oeiiee i?ioea??oniee ?ioi?oao i?e iaeii/ani?e
?ioi?caoe?? c AEIA.

Ia aenia?eiaioaeueieo iiaeaeyo iaa?oioiaaia iiaeeea?noue ei?aeoe??
aca?iii? ?ioiinoi?aneaii? ae?? aaeoeei AEIA oa AEAeI oeyoii ?ic’?aeiaiiy
?ioi?caoe?? a /an? (ia?oith aaiaeeoueny AEIA).

Aoaeoeai?noue cai?iiiiiaaii? noaie ?ic”?aeiaii? a /an? aaeoeeiaoe??
i?ioe iie?ii??e?oo, aeeooa??? oa i?aaoeth aoea aeiaaaeaia a i?ioean?
?ioi?caoe?? ae?oae i. I?eeoee.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a

Aaiaoe/ia iaiaeii??aei?noue iiioeyoe?e aaeoeeiieo ooai?a a??on?a
iie?ii??e?oo, aeenioe?aoe?y ?o a i?ioean? ?ai?iaeoeoe?? ia aa??aioe
Aaaio+ oa Aaaio(, aeniea aioeaaiia oa ?ioiiaaiia aeoeai?noue aa??aioa
Aaaio+, ?ioiinoi?aneaia ae?y aa??aioo Aaaio( aeathoue i?aenoaae aeey
oaeineiiaeaiiy AEIA. A oiiaao iaeaooiueiai noai?aiiy AEIA a Oe?a?i?
aeioe?eueii ai?iaaaeeoe naeaeoe?th ?c iiioeyoe?e aaeoeeiieo ooai?a
aa??aio?a Aaaio+, a?eueo — a?ieia?/ii aeoeaieo oa iaae?eaieo
i?ioaeoeaieie aeanoeainoyie.

C iaoith ciaioaiiy aca?iii? ?ioiinoi?an?? aeaio aaeoeei:AEIA oa
AEAeI_-aeioe?eueii ?icii/eiaoe oeeee ?ioi?caoe?? ae?oae aaeoeeiith AEIA,
iio?i iine?aeiaii, c ?ioa?aaeii 1 i?nyoeue ?ioi?coaaoe ii /a?c? AEAeI oa
AEIA. I?ney oaeeo ?ioi?caoe?e noai?th?oueny a?eueo ae?aaeaiee
aoii?aeueiee ?ioi?oao i?ae i?e iaeii/anieo aaeoeeiaoe?yo AEIA – AEAeI.

?ic?iaeai? iaoiaee/i? i?aeoiaee aei ei?aeoe?? eaeaiaea?th uaieaiue
aaeoeeiaie AEIA oa AEAeI ciiaeooue a cia/i?e i??? ni?eyoe i?aeaeuaiith
aoaeoeaiino? i?io?eaeoeee iie?ii??e?oo, aeeooa???, i?aaoeth oa
caa?aaeaiith caei?ia’y ae?oae a Oe?a?i?.

Ieaio?oueny ?icaeiooe cai?iiiiiaaio eiioeaioe?th ei?aeoe?? aaeoeeiaoe??
a oe?ieeo ai?aeai?/ieo aeine?aeaeaiiyo. Iaea?aeai? ?acoeueoaoe
ai?iaaaeeoe i?e noai?aii? iiaiai oaeineiiaeaiiai eaeaiaea?th uaieaiue
ae?oae a 2000 ?ioe?.

Iniiai? iieiaeaiiy aeena?oaoe??, ui aeiinyoueny ia caoeno

Aeenioe?aoe?y iiioeyoe?e a??on?a iie?ii??e?oo a i?ioean? ?o
?ai?iaeoeoe?? ? aacenii aeey naeaeoe?? ?c aeo?aeieo iiioeyoe?e
aaiaoe/ieo aa??aio?a c iio??aieie aeanoeainoyie. Aeey noai?aiiy ye?nieo
aaeoeei, ae?aaiinoe/ieo i?aia?ao?a ei?enieie ? aeenioe?aioe iiioeyoe??
iie?ia??on?a– aa??aioe Aaaio+. A ?ioiiiaoiaaiac? iie?ii??e?oo aa??aioe
Aaaio+. iathoue aeii?ioth/o ?ieue, ye iaea?eueo a??oeaioi? oa aioeaaii?.

A??one iie?ii??e?oo ? iiooaei?ie iiaeoeyoi?aie ?ioiii? a?aeiia?ae? aei
aaoa?ieia?/ieo aioeaai?a. I?iyaaie ?ioiiiiaeoeyoe?? iiaea aooe
?ioiinoeioeyoe?y, aai ?ioiinoi?an?y a caeaaeiino? a?ae aeaeo aioeaai?a.
Aoaioaoe?y iie?ia??on?a ia a?aei?iy? ?ioiiiiaeoeyoe??. A i?iyaao
?ioiinoi?an?? aeii?io? aaiaoe/iee aa??aio Aaaio(. Ia iiaeae? ieoae a?i
?iaeoeo? aiiioic ?ioiieiiiaoaioieo ee?oei naeac?iee.

Iniaenoee aianie caeiaoaa/a. O aeena?oaoe?? aeeeaaeaii ?acoeueoaoe
oai?aoe/ieo oa aenia?eiaioaeueieo aeine?aeaeaiue, ia?aaaaeii i?iaaaeaieo
aaoi?ii iniaenoi. O?aaiaioe ieaiiaeo oa a?aioiaeo IAe? aeeiiai?
iiooeoaa/ai ye aeeiiaaoeai oa a?aeiia?aeaeueiei aeeiiaaoeai aeuaiacaaieo
iaoeiaeo oai: Aeaeo?iiii-i?e?ineii?/i? aeine?aeaeaiiy aoee i?iaaaeai? ia
aac? eaai?aoi??? aeaeo?iiii? i?e?ineii?? IAeEOe ?acii c ae.a.i., i?io.
Noa/aiei E.I., aeaye? ?ioiieia?/i? aeine?aeaeaiiy aoee cae?eniai? a
eaai?aoi??? ?ioiieia?? ?inoeoooo iieaeoey?ii? a?ieia?? oa aaiaoeee IAI
Oe?a?ie noi?nii c e.i.i. Naithe I.O. Aeneiaeth?ii ?i ue?o iiaeyeo. An?
iieiaeaiiy oa aeniiaee aeena?oaoe?eii? ?iaioe iaeaaeaoue aaoi?o. Aaoi?
naiino?eii iaienaa oa i?iaiae?coaaa an? ae?oeiaai? iaoa??aee

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??. ?acoeueoaoe aeine?aeaeaiue aoee
i?aaenoaaeai? aeiiia?aeyie oa noaiaeaie ia VI,VII c”?caeao Oe?a?inueeiai
i?e?ia?ieia?/iiai oiaa?enoaa, 1984,1989?., IV iaoe?iiaeuei?e
eiioa?aioe?? ii aea?aieia?? oa ?ioaeoe?ei?e ?ioiieia??, Aa?ia, 1984,
Ananithci?e eiioa?aioe?? “ Aioa?ia??one. Caaaeueii oai?aoe/i? oa
iaaee/i? aniaeoe.”, 1991?., IV eiia?an? Na?oiai? oaaea?aoe??
oe?a?inueeeo e?ea?nueeeo oiaa?enoa, 1992?., i?aeia?iaei?e eiioa?aioe??
aei 150-??//y c aeiy ia?iaeaeaiiy ?.?.Ia/i?eiaa, 1995?., 10-io
?ioa?iaoe?iiaeueiiio eiia?an? c a??onieia??, 1996 ?., i?aeia?iaei?e
iaoeia?e eiioa?aioe?? “Ii?oieia?y-i?aeoe/i?e aaoa?eia??? oa
iaaeeoeei?”, 1998 ?.

Ioae?eaoe??. Ca oaiith aeena?oaoe?? iioae?eiaaii 30 noaoae a iaoeiaeo
aeo?iaeao oa ca??ieeao i?aoeue, aeaae a iiiia?ao??, 1 iaoaio ia
aeiao?ae, 1 iaoiaee/i? ?aeiiaiaeaoe??, 29 ?ia?o o iaoa??aeao c”?cae?a oa
eiioa?aioe?e.

Ai?iaaaeaeaiiy a i?aeoeeo. Iaea?aeai? ?acoeueoaoe ciaeoee canoinoaaiiy
i?e aeaeaii? iaoiaee/ieo ?aeiiaiaeaoe?e “I?eiaiaiea aaioiieoa aeey
auyaeaiey yioa?iae?onia o /aeiaaea e ai aiaoiae n?aaea”, i?ae. ?aae.
A.I.Oe?iaieiaa, Ee?a, 1986, IIC O?N?, a iaaeaaia?/iiio i?ioean? ia
eaoaae?? i?e?ia?ieia??, a??onieia?? oa ?ioiieia?? IIO. Oaeineiiaeaia
iiaa noaia ?ioi?caoe?? ae?oae i?ioe iie?ii??e?oo, aeeooa??? oa i?aaoeth
ai?iaaaeaeoaaeany a ?iaioo aeeoy/i? e?ea?i? i.I?eeoee. Iaoa??aee
aeena?oaoe?? aoee aeei?enoai? i?e i?aeaioiaoe? iaeaco IIC Oe?a?ie “I?i
iineeaiiy caoiae?a uiaei iiia?aaeaeaiiy caoai?thaaiino? ia iie?ii??e?o a
Oe?a?i?” ? 196 a?ae 14.07.1998 ?ieo.

Ianya ? no?oeoo?a aeena?oaoe??. Aeena?oaoe?y neeaaea?oueny ?c anooio,
iaeyaeo e?oa?aoo?e, iieno iaoa??ae?a oa iaoiae?a aeine?aeaeaiue, 5 aeaa
aeanieo aeine?aeaeaiue, caeeth/aiiy, aeniiae?a, i?aeoe/ieo
?aeiiaiaeaoe?e oa nieneo aeei?enoaii? e?oa?aoo?e. ?iaioo aeeeaaeaii ia
333 noi?. iaoeiiieniiai oaenoo, ?ethno?iaaiiai 32 oaae., 40 ?en.
A?ae?ia?ao?y neeaaea?oueny c 487 aeaea?ae, na?aae yeeo 143 oe?a?ii- oa
?in?enueeiiiaieo ? 344 ?iicaiieo aaoi??a.

IAOA??AEE OA IAOIAeE AeINE?AeAEAIIss.

Aieiaieie ia”?eoaie aoee aaeoeeii? oa ae?iaie/? ooaie a??on?a
iie?ii??e?oo ?, ?? oa ??? oei?a, io?eiai? c ?inoeoooo iie?ii??e?oo oa
a??onieo aioeaoae?o?a AII N?N? a 1982 ?ioe?. Aaeoeeii? ooaie:
Picornaviridae, Enterovirus, Polio; oei ?, ooai LSn2ab, aoaioeiaaiee
Naa?iei 1956-1957?. Iioiaeeoue a?ae aeeeiai a??ono Iaaiiae.
Picornaviridae, Enterovirus, Polio; oei ??, ooai P-712,ch 2ab,
aeae?eaiee Aaeueoaiaeii, Oienii c oaeae?e caei?iai? aeeoeie,.
aoaioeiaaiee Naa?iei a 1957?. Picornaviridae, Enterovirus, Polio; oei
???, ooai Leon12 a,b, aoa aoaioeiaaiee Naa?iei a 1956-1957?., iioiaeeoue
?c ooaia Leon, aeae?eaiee ?c iiceo aeeoeie, iiia?ei? a?ae iie?ii??e?oo.
Ianaae? aaeoeeiieo ooai?a i?iaiaeeee i?e oaiia?aoo?? +340N, caa??aaee
i?e oaiia?aoo?? -200C. A??oeaioi? ooaie: Picornaviridae, Enterovirus,
Polio; oei ?, ooai Mahoney,. Picornaviridae, Enterovirus, Polio; oei ?I,
ooai MEF-1, Picornaviridae, Enterovirus, Polio; oei II?, ooai Saukett.

Eoeueoo?e ee?oei: ia?auaiethaai? eoeueoo?e ee?oei oei?a Vero — ee?oeie
ie?ie ao?eeainueei? caeaii? iaaie, HAp-2- ee?oeie ea?oeeiiie ai?oai?
ethaeeie, L-929- iae?ai?ciaaia i?aeoe??ia niieo/ia oeaieia ieoae e?i??
N3I..

Aaeoeeii? oa ae?aaiinoe/i? i?aia?aoe: aaeoeeia iie?ii??e?oia ia?i?aeueia
?, ??, ??? oei?a – o?eaaeaioiee i?aia?ao ?c aoaioeiaaieo ooai?a Naa?ia
a??on?a iie?ii??e?oo, na??y ?451. Iineaa EIAY ?i. I.I.*oiaeiaa
?AII.Poliovirus vaccine inactivated – IPOL. Produced by Pasteur Merieux
Serums & Vaccins S.A..Adsorbed diphtheria, tetanus and pertussis
vaccine. Produced by Pasteur Merieux Serums & Vaccins. Lot: 5429.
Ne?iaaoee ae?aaiinoe/i? iie?ia??oni? oeiiniaoeeo?/i? noo? aeey ?aaeoe??
iaeo?ae?caoe??, oeie – ?, ??, ???. Iineaa EIAY.ei. I.I.*oiaeiaa ?AII.
Ae?aaiinoe/iee eiiieaeo “i?e?iaiae?c – aeeooa??y, i?aaaoeue”. I?noeoue
aeeooa??eiee aioeaaiiue (aiaoieneiiee) a?eo?ioeeoa?iee ae?aaiinoeeoi,
i?aaoeaaee aioeaaiiee (aiaoieneiiee) a?eo?ioeeoa?iee ae?aaiinoeeoi.
Noai?aiee a ?inoianueeiio-ia-Aeiio iaaee/iiio ?inoeooo?, oeaio?aeueiiio
IAe? ai?aeai?ieia?? IC N?N?.

Aenia?eiaioaeuei? oaa?eie: ieo? e?i?e BALB/n, NBA, N57Bl, naioe? oa
naieoe?, ianith 18 — 20 a?ai?a, iaea?aeai? ?c ?icie?aeieeo a?aa??th
?inoeoooo aenia?eiaioaeueii? iieieia?? oa ?aae?ieia??
?i.?.?.Eaaaoeueeiai IAI Oe?a?ie, ?inoeoooo i?e?ia?ieia?? oa a??onieia??
?i.Ae.E.Caaieioiiai IAI Oe?a?ie. Ea?aioei ia??iaeo aee?iaoecaoe??,
oo?eiaiiy oaa?ei i?ae /an aenia?eiaioo, i?e?iee?iao, oaiia?aoo?a,
aieia?noue, na?oeiaee ?aaeei, ia`?i iia?o?y, oei ee?oei, a?aeiia?aeaee
caaaeueiii?eeiyoei ii?iaoeaai oo?eiaiiy oaa?ei a?aeiia?aeii
?a?iiaenueei? eiiaaioe?? ii caoenoo o?aaoiaeo oaa?ei, ui
aeei?enoiaothoueny c aenia?eiaioaeueiith iaoith. Anueiai aeei?enoaii
1770 ieoae.

Iaoiaee a??onieia?/ieo aeine?aeaeaiue in vitro. Ianaae? ee?oei
i?iaiaeeee ca caaaeueiii?eeiyoeie iaoiaeaie, iiiioa?e ee?oei
iaea?aeoaaee a neeyieo oeaeiiao aai iao?anao i?e aeei?enoaii?
na?aaeiaeua ?inoo( noi?o? c ??aieo /anoei na?aaeiaeu 199 oa ?aea c
aeiaeaiiyi 8%-10% ne?iaaoee aaeeei? ?iaaoi? ooaeiae aai 7%
aia??iiaeueii? ne?iaaoee c aioea?ioeeaie iai?oeee?iii oa no?aioii?oeeiii
-100 iae.ia ie). Ee?oeie eoeueoeaoaaee i?e 36,60N , i?e ianaaeao (/a?ac
2-3 aeiae) iiiioa?e ia?iaeyee 0,02% ?ic/eiii Aa?naio aeey a?aeae?eaiiy
a?ae neea. Ianaaee a??on?a i?iaiaeeee c a??onai?niei iaoa??aeii a
e?eueeino?, ui caaacia/oaaea aenieo iiiaeeii?noue ?io?eoaaiiy iiiioa??a
ee?oei. Aaeni?aoe?y a??on?a-30 oaeeei, ?ieoaaoe?y i?e -36,60N aeey
a??oeaioieo oa -340N, aeey aaeoeeiieo ooai?a, 48-72 aiaeeie aei ianoaiiy
iiaiiai oeeoiiaoiaaiiiai aoaeoo.

Iaea?aeaiiy eiioeaio?iaaieo i?aia?ao?a a??on?a i?iaiaeeee ca iaoiaeii
(A.I. Oe?iaieia, 1973; 1977) ?c canoinoaaiiyi i?e?iaeiiai ni?aaioo
aaioii?oo. I?eioeei iieyay? o aeei?enoaii? aeanoeaino? aioa?ia??on?a
aaeni?aoaaoeny ia aae? aaioii?oo i?e eeneeo ?I (4,5-5,0). I?e eoaeieo ?I
(8,0-9,0) oa a?aenooiino? aeaooaaeaioieo eao?ii?a aioa?ia??one caeaoi?
aeani?aoaaoeny a aethth/ee ?ic/ei. Oaeei /eiii ?c ia”?i?a -500 — 1000 ie
a??onai?niiai iaoa??aeo iiaeia iaea?aeaoe cia/ii iaio? 5 — 10 ie,
eiioeaio?aoe?y a??on?a c?inoa? ia aeaa-o?e eiaa?eoie.

Oeo?e a??on?a aecia/aee: ca aeyoeiooai?aiiyi i?ae aaioii?oiaei iie?eooyi
(A.I. Oe?iaieia, 1973) oa i?e?iiaoiaeii ca OeIA (?oeiaiaenoai ii
ae?onieiae/aneei iaoiaeai, Iineaa, 1996; Manual for the virological
investigation of polio, WHO, 1997), i?eioeei iaoiae?a: ?ioaeoe?ei?
a??one i?ae /an ?ai?iaeoeoe?? ni?e/eiythoue aeaeeiee OeIA — iiaio
aeano?oeoe?th ee?oei, ?oeioaaiiy iiiioa?o. I?e canoinoaaiiy aaeth
aaioii?oo ooai?ththoueny ciie aeano?oeoe?? iiiioa?o ee?oei- aeyoee,
aaaaeathoue, ui aeyoea ? ?acoeueoaoii ?ai?iaeoeoe?? iaeiiai a??ono,
e?euee?no?ue a??on?a ae?aaeaee a aeyoeiooai?thth/eo iaeeieoeyo-AOI.

?aeaioeo?eaoe?y oei?a a??on?a iie?ii??e?oo. Aeey i?aeoaa?aeaeaiiy
iaeaaeiino? aei na?ioei?a ?, ??, ??? a??on?a iie?ii??e?oo
aeei?enoiaoaaee ?aaeoe?th iaeo?ae?caoe?? a??on?a (?IA) (Manual for the
virological investigation of polio, WHO, 1997), i?eioeei iaoiaeo iieyaa?
a oiio, ui i?e eiioaeo? a??on?a c niaoeeo?/ieie aioeo?eaie a??one
ao?a/athoue naith ?ioaeoe?eio aeoeai?noue. Oea ia?aa??ythoue i?e
?io?eoaaii? eoeueoo? ee?oei, aoaeo iaeo?ae?caoe?? a??on?a i?iyaey?oueny
ye a?aenooi?noue OeIA, a?aenooi?noue aeyoie.

Naeaeoe?th aaioii?oiaeo aaiaoe/ieo aa??aio?a a??on?a cae?enithaaee ca
i?ea?iaeueiei iaoiaeii (A.I.Oe?iaieia, 1977), ?ic?iaeaiei i?e a?aee?eoo?
oaiiiaio aeenioe?aoe?? aioa?ia??on?a oa aeine?aeaeai? eiai
caeiiii??iinoae. I?eioeei iaoiaeo iieyaa? a oiio, ui /anoeia iiioeyoe?e
aioa?ia??on?a ia? aenieee ao?i?oao aei ni?aaioo, oiio aaeni?aothoueny
i?e iaeo?aeueieo oa neaaieoaeieo ?I, oaeee aa??aio iiioeyoe?? iaea?aeaa
neiaie Aaaio+, ?ioa, iae/ano?oa i?ii?ia /anoeia iiioeyoe??, — a??one c
iecueeei ao?i?oaoii aei aaioii?oo — aa??aio Aaaio(.Caeaoi?noue
aioa?ia??on?a aei aaeni?aoe?? ia aaioii?o? ?aeiiaiaeiaaia aeey
aecia/aiiy aaioii?oiaiai ia?ea?o a??oeaioiino?
iie?ia??on?a.(I.E.Ai?ioeeiaa, 1979).

A??onieia?/i? oa ?ioiieia?/i? aeine?aeaeaiiy in vivo. Aecia/aiiy
iae?ia??oeaioiino? a??on?a iie?ii??e?oo ?? oeio i?iaiaeeee oeo?oaaiiyi
a??on?a a iiceo ieoae. I?eioeei iaoiaeo: i?e ca?aaeaii? ieoae ??cieie
aeicaie a??on?a ca i?iyaaie ?ioaeoe?? oa eaoaeuei?noth ciaoiaeyoue
i?i?iaeueio e?euee?noue a??on?a, yea ni?e/eiy? 50% caaeaae? oaa?ei –
LD50 aai 95% — DLM, i?ae?aoiaothoue ca iaoiaeii ??aea oa Iai/a
eoioeyoeai? aeai? eaoaeueiino? oa na?aaeiuei? o?eaaeino? aeeooy
(C.A.Ioaie/iia a niaao., 1974; I.I.?aeueo a niaao., 1982). Aeae?eaiiy
a??on?a iie?ii??e?oo ?c i?aai?a ieoae i?iaiaeeee aeey aeieaco ?o
?ai?iaeoeoe?? a ??cieo i?aaiao. Iaoa??aeii aeey a??onieia?/iiai
aeine?aeaeaiiy aoee oiaoi/ee i?aai?a, a yeeo aecia/aee e?euee?noue
a??on?a iaoiaeii oeo?oaaiiy ia eoeueoo?? ee?oei IA?-2, ae?aaeaee AOI ia
1 a?. oeaieie.

Ioe?ieo aioeaaiiino? a??on?a iie?ii??e?oo cae?enithaaee i?e
aioo??oiuei/a?aaeii?e ?ioi?caoe?? ieoae iaeiaeiaeie aeicaie a??on?a. Ooo
? aeae? oa?i?i ?ioi?caoe?y oiiaii aeei?enoiaoaaee aeey aecia/aiiy
i?ioean?a, ui aeieeathoue aiane?aeie aaaaeaiiy a i?aai?ci aeeeeo
iie?ia??on?a aai aeeaeo iie?ia??onieo aaeoeei. Ia 3, 5, 7 aei? i?ney
?ioi?caoe?? ieoae caaeaaee, a?aee e?ia, aeae?eyee ne?iaaoeo, aecia/aee
iayai?noue i?ioea??onieo aioeo?e oa ?o oeo?e ca aeiiiiiaith ?IA (?iaeaen
iaeo?ae?caoe?? 80%). Aeey ii??aiyiiy ??ai?a a??oniaeo?ae?coth/eo aioeo?e
a ??cieo a?oiao aenia?eiaioaeueieo oaa?ei ?ic?aoiaoaaee na?aaei?
aaiiao?e/i? aaee/eie oeo??a, ae?aaeaee ?o o eiaa?eoiao i?e iniia? 2,
oaeiae ae?aaeaee oeo?e a na?aaei?o aaee/eiao cai?ioi?o ?icaaaeaiiyi
ne?iaaoie (I.I.Ieioea? a niaao., 1982).

Ioe?ieo ?ioiiaaiii?( i?ioaeoeaii? i?e ?ioaeoe??) ae?? a??on?a
iie?ii??e?oo aecia/aee aeey aaeoeeiieo ooai?a a??on?a iie?ii??e?oo
??oeio oa ?o aaiaoe/ieo aa??aio?a. Aeey oeueiai ieoae ?ioi?coaaee
aioo??oiuei/a?aaeiii aeicaie a??on?a – 1o106AOI. *a?ac 3 aeiae
i?iaiaeeee ?io?aoea?aa?aeueia ca?aaeaiiy a??oeaioiei aeey ieoae ooaiii
iie?ia??ono ?? oeio – MEF-1 – a LD50.A eiaei?e a?oi? ieoae aecia/aee
na?aaeith o?eaae?noue aeeooy, eaoaeuei?noue ii??aithaaee ?o c aaee/eiaie
a eiio?ieuei?e a?oi? oa ?ic?aoiaoaaee i?ioaeoeaio ae?th aaeoeeiieo
iie?ia??on?a.

Aea/aiiy aoii?aeueiiai ?ioi?oaoo i?iaiaeeee a i?ioean? ?ioi?caoe?? ieoae
a??onaie iie?ii??e?oo. Ia 3, 5, 7, 14, 21 aeaiue aeine?aeaeoaaee
ne?iaaoee e?ia? ca aeiiiiiaith ?IA aeey aeyaeaiiy i?ioea??onieo
oeiiniaoeeo?/ieo aioeo?e oa ?o oeo??a.

Ee?oeiiee ?ioi?oao ioe?ithaaee ca ooieoe?iiaeueiith aeoeai?noth
?ioiieiiiaoaioieo ee?oei naeac?iee (J.Robinson et al., 1975; A.Oannaiia,
1979; A.O?eiaeue, 1987). Naeac?iee ieoae caae?aee ia ??ci? oa?i?ie i?ney
?ioi?caoe?? a??onaie iie?ii??e?oo aai aaeoeeiaie .Nieaiioeeoe aeae?eyee
a a?aae??io? u?eueiino? o?eiee-aa?ia?ao?io c iieacieeii 1,076-1,078,
eoeueoeaoaaee a iiaeeaiiio na?aaeiaeu? RPMI 1640 c 10% ai?noii
aia??iiaeueii? oaey/i? ne?iaaoee, 2iI aeooai?io, no?aioii?oeeio oa
iai?oeee?io ii 100 iae/ie, a i?enooiino? 5%NI2 .Aeei?enoiaoaaee i?oiaaie
OAA oa EIN a eiioeaio?aoe?? 5 iea/ie, aai ?iaeoeaiaai? oi?iae?iii
iie?ia??one ca iaoiaeii (B.Montagon et al., 1985) 3I-oei?ae?i aeiaeaaaee
ca 4 aiaeeie aei cae?i/aiiy eoeueoeaoaaiiy c ?ic?aooieo 2 ieE? ia eoieo,
ee?oeie inaaeaeoaaee ia i?o?ioeaetheueici? o?eueo?e(ae?aiao? ii?
0,45iei). Ooieoe?iiaeueio aeoeai?noue e?ioioeeo?a aecia/aee ca
aeeth/aiiyi 3I-oei?ae?io a AeIE ca aeiiiiiaith noeeio?eyoe?eiiai
e?/eeueieea.

Ianeaiee ia?aiin ?ioi?oaoo c ne?iaaoeaie e?ia? (aioeo?eaie):ieoae
?ioi?coaaee o?e/? aioo??oiuei/a?aaeiii a??onaie iie?ii??e?oo ?? oeio
ooaiaie MEF-1 oa P-712ch2ab a aeic? 1o106. Ia 14 aeaiue i?ney inoaiiuei?
?ioi?caoe?? o ieoae caae?aee e?ia, iaea?aeoaaee ne?iaaoeo, aecia/aee a
i?e i?ioea??oni? aioeo?ea oa ?o oeo?e a ?IA. Iaea?aeai? ne?iaaoee c
aioeo?eaie a oeo?? 512 aaiaeeee a oainoiao aaio a ia`?i? 0,2 ie.

Aaeiioeaiee ia?aiin ?ioi?oaoo c e?ioioeeoaie: o ?ioi?ciaaieo a??onaie
iie?ii??e?oo ?? oeio ieoae iaea?aeoaaee nieaiioeeoe (ye iienaii aeua),
5o107 ee?oei aaiaeeee aioo??oiuei/a?aaeiii ieoai-aeiii?ai.

Aea/aiiy ?ioiiiiaeoethth/i? ae?? a??on?a iie?ii??e?oo: ieoae
?ioi?coaaee a??onaie iie?ii??e?oo ?, ??, ??? oei?a oa ?o aaiaoe/ieie
aa??aioaie, aaeoeeiieie oa a??oeaioieie ooaiaie, a iaeiaeiaeo aeicao –
1o106, aioo??oiuei/a?aaeiii. Ia oii? a??onii? ?ioi?caoe?? cae?enithaaee
?ioi?caoe?th ieoae aaoa?ieia?/ieie aioeaaiaie, iio?i aecia/aee ci?ie
?ioiii? a?aeiia?ae? aei ieo, iaoiiaeai? ?ioiiiiaeoethth/ith ae??th
iie?ia??on?a.

Ioe?iea aieeao ?ioi?caoe?? a??onaie iie?i??e?oo ia aioeo?eiooai?aiiy aei
aaoa?ieia?/ieo a?eo?ioeeo?a: ia oii? ?ioi?caoe?? iie?ia??onaie
i?iaiaeeee ?ioi?caoe?th a?eo?ioeeoaie aa?aia. E?euee?noue
aioeo?eiooai?thth/eo ee?oei (AOE) aei a?eo?ioeeo?a aa?aia aecia/aee
iaoiaeii eieaeueiiai aaiie?co a aae? (N.Jerne et al., 1963), i?eioeei
yeiai iieyaa? a oiio, ui e?ioioeeoe ?ioi?ciaaieo aaoa?ia?eo?ioeeoaie
oaa?ei i?iaeoeothoue aioeo?ea-aaiie?ceie. ?aaeoe?th ioe?ithaaee ii
e?eueeino? cii aaiie?co a?eo?ioeeo?a c AOE a oeaio??.

Ioe?iea aieeao ?ioi?caoe?? a??onaie iie?ii??e?oo ia ee?oeiio ?aaeoe?th
i?aeaeuaii? /ooeeaino? niia?eueiaiiai oeio aei aaoa?ieia?/ieo
oeiioeeo?a: ia oii? ?ioi?caoe?? a??onaie iie?ii??e?oo iiaeaethaaee
aea?a?/io ?aaeoe?th ia aaoa?ieia?/i? oeiioeeoe. Aeey oeueiai ia?aee
iaoiae a?aeoai?aiiy oa ioe?iee ?aaeoe?? i?aeaeuaii? /ooeeaino?
niia?eueiaiiai oeio (I*NO) in vivo (Y.A.Atheeeia a niaao., 1981).
I?eioeei oe??? ?aaeoe?? iieyaa? a oiio, ui o ieoae, nainea?e?ciaaieo
oeiioeeoaie iaoethe?a, ia aeicaieyth/o aeico oeiioeeo?a ?icaeaa?oueny
?aaeoe?y I*NO, yea /?oei ?a?no?o?oueny ii ci?i? iane ?aa?iia?ieo
e?ioaoe/ieo aoce?a. ?ioi?ciaaieo ieoae nainea?e?coaaee oeiioeeoaie
iaoethe?a (1o104, a iiaeooa/ee iaio caaei?o eai). *a?ac 4 aeiae aaiaeeee
aeicaieyth/o aeico oeiioeeo?a – 1o106, a i?aao eaio – aeine?ae, a e?ao
eaio — 1o106 oeiioeeo?a ii?nueei? naeiee – eiio?ieue. *a?ac 1 aeiao
ieoae caaeaaee, aeeo/aee i?aeeie?ii? e?ioaoe/i? aocee, aecia/aee
??cieoeth a ian? aeine?aeieo oa eiio?ieueieo a %, ui na?ae/eei i?i
nooi?iue i?iyao I*NO.

Aea/aiiy aca?iiiai aieeao ?ioi?caoe?? iie?ii??e?oieie aaeoeeiaie oa
AEAeI ia ooai?aiiy aoii?aeueiiai aioeoiene/iiai oa i?ioea??oniiai
?ioi?oaoo. Ieoae ?ioi?coaaee AEIA aai ?IA iaeii/anii c aaeoeeiith AEAeI
aioo??oiuei/a?aaeiii, ii iaei?e aeic? eiaeii? aaeoeeie. Ia 7, 14 aeiao
ieoae caaeaaee, a?aee e?ia, iaea?aeoaaee ne?iaaoee, a yeeo aecia/aee
aioeo?ea aei ie?aieo eiiiiiaio?a aaeoeei. A??oniaeo?ae?coth/? aioeo?ea
aeyaeyee ca aeiiiiiaith ?IA. Aeeooa??ei? oa i?aaoeaa? aioeoieneie
aeyaeyee a ?IAA (Eniieueciaaiea eiioiieiae/aneeo enneaaeiaaiee a
i?ioeeaeoeea eioaeoeee, oi?aaeyaiuo aaeoeeiaoeeae,-Iineaa, 1991;
Recommendations of the Immunization Practices Advisory committee, 1991).

I?eioeei ?IAA iieyaa? a oiio, ui niaoeeo?/i? aioeoiene/i? aioeo?ea
ni?e/eiythoue aaiaaethoeiaoe?th a?eo?ioeeo?a aa?aia, aeei?enoaieo a ?ie?
iin??a a?aeiia?aeieo aioeaai?a- aeeooa??eieo aai i?aaoeaaeo aiaoienei?a
Aeine?aeaeoaai? ne?iaaoee ?icaiaeeee a 5 ?ac?a ?cioii?/iei ?ic/eiii
oei??aea iao??y (?I=7,2), iio?i ?iaeoeaoaaee i?e 560N 30 oaeeei,
ia?iaeyee oi?iae?i?ciaaieie a?eo?ioeeoaie aa?aia (50,0%) aeey aeaeaeaiiy
aaoa?io?eueieo aioeo?e. Aiooaaee a?aeiia?aei? ?icaaaeaiiy ne?iaaoie a?ae
1:10 aei 1:1280, a eiaeia ?icaaaeaiiy aeiaeaaaee ii 0,025 ie
a?aeiia?aeiiai ae?aaiinoeeoio. Eiio?ie?: ae?aaiinoeeoi?a ia
a?aenooi?noue niiioaiii? aaethoeiaoe??, ne?iaaoee ia a?aenooi?noue
aaethoei?i?a aei a?eo?ioeeo?a aa?aia. ?acoeueoaoe ioe?ithaaee ca
nooiaiai aaethoeiaoe?? a?eo?ioeeo?a. Aeoeai?noue a?eo?ioeeoa?ieo
ae?aaiinoeeoi?a : 1-aeeooa??eiee (aeey ?IAA) – ?c noaiaea?oiith
ne?iaaoeith 1:3200- 1:6400; 2- i?aaoeaaee (aeey ?IAA)– ?c noaiaea?oiith
ne?iaaoeith 1:1280- 1:2560. I?e aeine?aeaeaii? ne?iaaoie a ?IAA
aeoeai?noue aeeooa??eiiai ae?aaiinoeeoio -1:3200, i?aaoeaaiai – 1:1280.
Oea aea? iiaeeea?noue ia?aaiaeeoe iaea?aeai? a ?icaaaeaiiyo ?acoeueoaoe
a i?aeia?iaei? iaeeieoe? (II), a naia: oeo? 1:10- 0,03 II/ie; 1:20-
0,06II/ie, 1:40- 0,12II/ie, 1:80- 0,24II/ie, 1:160- 0,48II/ie, 1:320-
0,96II/ie. I?e ioe?ioe? aioeoiene/iiai ?ioi?oaoo o ae?oae aaaaeaee, ui
a?oio na?iiaaaoeaieo neeaaeaee iniae, o yeeo ia aeyaeyee a?aeiia?aeieo
aioeo?e a ne?iaaoeao. A?oio iacaoeuaieo neeaaeaee iniae c oeo?ii ieae/a
caoeniiai, a?aoiaoaaee oaeiae na?iiaaaoeaieo. I?ioaeoeaiei aeey
aeeooa??? aaaaea?oueny oeo? 1:40, aeey i?aaoeth- 1:20.

Noai?aiiy iiaeaeae aaeoeeiaeueiiai i?ioeano c aeei?enoaiiyi
iie?ii??e?oieo aaeoeei oa AEAeI. C iaoith aenia?eiaioaeueiiai
iaa?oiooaaiiy i?aeaeuaiiy aoaeoeaiino? aaeoeeiaoe?? i?ioe iie?ii??e?oo,
aeeooa??? oa i?aaoeth aoee noai?ai? ??ci? noaie ?ioi?caoe??:
1-Iaeii/ania ?ioi?caoe?y AEIA oa AEAeI. 2-Iaeii/ania ?ioi?caoe?y ?IA oa
AEAeI. 3-?ioi?caoe?y AEIA – ?ioa?aae 7 ae?a – ?ioi?caoe?y AEAeI.
4-?ioi?caoe?y AEIA – ?ioa?aae 14 ae?a – ?ioi?caoe?y AEAeI.
5-?ioi?caoe?y AEIA – ?ioa?aae 21 aeiaa – ?ioi?caoe?y AEAeI. Eiio?ieuei?
a?oie- ?ioi?caoe?y ie?aii AEIA, ?IA, AEAeI. An? aaeoeeie aaiaeeee a
iaei?e aeic?, /a?ac 7-14 aei?a i?ney inoaiiuei? ?ioi?caoe?? ieoae
caaeaaee, a?aee e?ia oa aeine?aeaeoaaee ?? ia iayai?noue aioeo?e oa ?o
oeo??a.

Aeaeo?iiii-i?e?ineii?/i? iaoiaee aeine?aeaeaiiy oeueo?ano?oeoo?e
?ioiieiiiaoaioieo ee?oei ieoae. Aeei?enoiaoaaee eeane/io iaoiaeeeo
i?eaiooaaiiy aeaeo?iiiii?e?ineii?/ieo i?aia?ao?a ?c oiaoi/e?a oeiono,
naeac?iee. Iaoa??ae o?enoaaee o 2% ?ic/ei? OsO4 ii Eieo?eueaeo ia
i?ioyc? 2 aiaeei i?e oaiia?aoo?? +4oN. C?acee ciaaiaeithaaee a aoaiie?
c?inoath/i? eiioeaio?aoe??, ii/eiath/e c 700 nie?oo, aoeaoiio oa
caeeaaee o noi?o aiiio oa a?aeae?oo ca caaaeueiia?aeiiith noaiith
(A.ss.Ea?oio, 1984). C??ce aeaioiaeyee ia oeueo?aoiiao LKB-O oa Reichert
? eiio?anooaaee 2% ?ic/eiii o?ai?eaoeaoaoo o 70% aoaiie? oa oeeo?aoii
naeioey oa aea/aee a aeaeo?iiieo i?e?ineiiao AIA-100A, AIA-100A?.
Ioe?ieo ci?i ii?oiooieoe?iiaeueii? i?aai?caoe?? ee?oei, iciaee aiiioico
a e?ioioeeoao i?iaiaeeee ca?aeii iaoiaeai (?.Eniaeeia, 1979;
A.I.Ni?ieei, 1989; R.Duke et al., 1996; S.Bergese et al., 1997).

Aea/aiiy aieeao ?ioi?caoe?? aeeaith iie?ii??e?oiith aaeoeeiith ia
ia?aa?a noao?eieieiai? ?ioaeoe?? o ieoae. Iiaeaeue noao?eieieico c
ee?i?/ieie i?iyaaie a?aeoai?thaaee i?e ?io?eoaaii? Staphylococcus aureus
Cowan 1, a aeic? 109 aaeoa??e, aaeaioiaaieo aei ieoae ( ooai io?eiaee c
eieaeoe?? Aea?aeaaiiai eiio?ieueiiai ?inoeooo? iaaee/ieo oa a?ieia?/ieo
i?aia?ao?a ?i. E.A.Oa?anaae/a, ?in?y). Ieoae caaeaaee oeyoii
oea?a?eaeueii? aeeneieaoe??, i?iaiaeeee ?icoei oa acyooy a anaioe/ieo
oiiaao e?ia? oa oiaoi/e?a i?aai?a. Aeey ioe?iee iaoiaaiaoe/ieo
iniaeeainoae noao?eieieico ia oii? ?ioi?caoe?? iie?ia??onaie ioe?ithaaee
i?e?iaia ian?i`yi?iiy e?ia?, na?oey, eaaai?a, ia/?iee, naeac?iee a
aeeiaieoe?. Aeey oi?o?eaoe?? e?euee?noue i?e?ii?aai?ci?a ia?aaiaeeee a
iieaciee, ui oa?aeoa?ecoaaa i?e?iaia ian?i`yi?iiy ia 1 a i?aaio /e 1 ie
e?ia? Aaeoa??? i?e aen?aao ?aeaioeo?eoaaee ca eeane/ieie iaoiaeaie. O
ca?aaeaieo noao?eieieaie ieoae aecia/aee oaaioeeoa?io aeoeai?noue
yae?iai?nieo ee?oei e?ia? ca eeane/iei iaoiaeii (TH.Aeaa, 1989).

Ioe?iea ?ioa?oa?iiiaaiii? aeoeaiino? AEIA oa AEAeI. Aeei?enoiaoaaee 2
i?aeoiaee aei aecia/aiiy ?OI: (O.E.A?oia, 1996) 1-Aeyaeaiiy aiaeiaaiiiai
ne?iaaoeiaiai ?OI, ?iaeoeiaaiiai aaeoeeiaie..2-Aeyaeaiiy neioaciaaiiai
nieaiioeeoaie i?ae ae??th ?iaeoeoi??a: eiieaiaaae?io A- (-?OI oa a??ono
oai?iae Iuetheanoea — (-?OI. I?eioeei iaoiaeo iieyaa? a oiio, ui ?OI ia?
i?ioaeoeaio ae?th ii a?aeiioaiith aei ee?oei, ?io?eiaaieo a??onaie. Oeae
aoaeo niinoa??aaee ii iaeo?ae?caoe?? OeIA i?i?ioi ia 50%.,
aeei?enoiaoaaee caaaeueiii?eeiyoo nenoaio: eoeueoo?o ee?oei L-929 oa
a??on aac?eoey?iiai noiiaoeoo (AAN).

Iaoiaee noaoenoe/ii? ia?iaee iaea?aeaieo ?acoeueoao?a. Aiae?c
?acoeueoao?a aeine?aeaeaiue i?iaiaeeee c aeei?enoaiiyi iaoiae?a
iaoaiaoe/ii? aa??aoe?eii? noaoenoeee ia IE 486 DX 2 — 66 AMD i?e
aeei?enoaii? i?ia?aiieo i?iaeoeo?a o??ie Microsoft.. Iniiaiith iciaeith
aei aeniiaeo i?i ??cieoeth i?ae aeaiia noeoiiinoyie aoa nooi?iue
?icoiaeaeaiiy ?o na?aaei?o aaee/ei. Aeey ioe?iee aeinoia??iino? ??cieoe?
iieaciee?a aecia/aee t-e?eoa??e Nouethaeaioa. Oaeiae canoiniaoaaee
iaoiae ioe?iee ??cieoe? i?ae /anoioaie iiyae iciaee a ie?aieo na??yo
niinoa?aaeaiue (A.E?aia?, 1975; I.I.Ieioea? a niaao., 1982).

?ACOEUeOAOE AEANIEO AeINE?AeAEAIUe

Aaoa?iaaii?noue iiioeyoe?e a??on?a iie?ii??e?oo ia i?eeeaae?
aeenioe?aoe??. Aacith aeey i?iaaaeaiiy aeine?aeaeaiue ? ?aae?caoe??
iinoaaeaieo caaaeaiue, iia’ycaieo ?c aea/aiiyi ?ioiiiaoiaaiaco oa
a?ieia?/ieo aeanoeainoae a??on?a iie?ii??e?oo ia iiioeyoe?eiiio ??ai?
aoei iaea?aeaiiy oeyoii naeaeoe?? c aeo?aeieo iiioeyoe?e a??oeaioieo oa
aoaioeiaaieo ooai?a a??on?a iie?ii??e?oo ?, ??, ??? oei?a aaiaoe/ieo
aaioii?oiaeo aa??aio?a. I?iaaaeaia naeaeoe?y i?iaeaiiino?oaaea
aaoa?iaaii?noue iiioeyoe?e iie?ia??on?a oa aeicaieeea iaea?aeaoe
aeenioe?aioe a??oeaioieo oa aaeoeeiieo a??on?a iie?ii??e?oo Aaaio+ oa
Aaaio( (oaae.1).

Oaaeeoey 1

Naeaeoe?y aaiaoe/ieo aaioii?oiaeo aa??aio?a ?c iiioeyoe?e a??on?a
iie?ii??e?oo

Oeie oa ooaie a??on?a

Oeo?e a??on?a aei aaeni?aoe?? ia aaioii?o? (n=20) Oeo?e a??on?a i?ney
aaeni?aoe?? ia aaioii?o? (n=20) Ai?no aa??aio?a Aaaio(a % Ai?no
aa??aio?a Aaaio+ a %

A??oeaioi? a??one iie?ii??e?oo (ee?oeie HEp-2)

?oei–Mahoney (9,85+0,012) o105 (9,65+0,01) o105 97,91(1,9 2,09(0,02

?? oei MEF-1 (9,61+0,017) o106 (3,5+0,015) o104 0,36(0,03 99,64(1,4

??? oei Saukett (9,54+0,026) o105 (3,33+0,011) o103 0,39(0,01 99,61(0,35

Aoaioeiaai? a??one iie?ii??e?oo (iocaei? ooaie, ee?oeie Vero)

? oei LSC2ab (6,0+0,033) o105 (1,38+0,011) o103 0,23(0,02 99,77(0,49

?? oei P712ch2ab (4.5+0,014) o105 (2,25+0,09) o102 0,05(0,001 99,95(0,27

III oei Leon12ab (1,0+0,027)o107 (9,98+0,031) o103 0,1(0,002 99,9(0,18

Aoaioeiaai? a??one iie?ii??e?oo (aaeoeeii? ae?iaie/? ooaie, ee?oeie
Vero)

? oei LSC2ab (6,0+0,044) o105 (4,3+0,015) o103 0,71(0,05 99,29(0,33

?? oei P712ch2ab (7.5+0,029) o106 (4,2+0,016) o104 0,57(0,004 99,43(0,09

??? oei Leon12ab (1,0+0,042) o106 (1,24+0,051) o104 1,25(0,01 98,75(0,12

Aoaioeiaai? a??one iie?ii??e?oo (aaeoeeii? ae?iaie/? ooaie, ee?oeie
HEp-2)

?oei – LSC2ab (1,1+0,019) o105 (6,5+0,026) o103 5,9(0,02 94,1(1,13

??oei

P712ch2ab (8,6+0,063) o107 (1,72+0,013) o104 0,02(0,001 99,98(0,76

??? oei Leon12ab (2,4+0,059) o106 (1,68+0,018) o104 0,70(0,05 99,3(0,45

I?ei?oea: i?e ii??aiyii? oeo??a a??on?a aei oa i?ney aaeni?aoe?? ia
aaioii?o? ?(0,001

A oaae.1 iaaaaeaia ocaaaeueiaia ?ioi?iaoe?y i?i naeaeoe?th aaiaoe/ieo
aaioii?oiaeo aa??aio?a a??on?a iie?ii??e?oo, yeo ie iino?eii
cae?enithaaee ia i?ioyc? 15 ?ie?a. Aeey ?ioa?i?aoaoe?? aoee aeei?enoai?
oaeoe i?i ai?no a iiioeyoe?yo aaiaoe/ieo aa??aio?a Aaaio(, ye? iaea?eueo
caeiiii??ii iiaoi?thaaeeny. Na?aae a??oeaioieo ooai?a iaaecae/aeia
e?euee?noue aa??aioo Aaaio( aoea ca?a?no?iaaia aeey a??on?a iie?ii??e?oo
? oeio, ooaio Mahoney – 97,91%, oiaoi oeae a??on i?aeoe/ii ?nio? a
“i?ionia?e”oi?i?. Iiioeyoe?? ?? oa ??? na?ioei?a i?noyoue i?aeoe/ii
iaeiaeiao e?euee?noue aa??aio?a Aaaio(-0,36 oa 0,39% a?aeiia?aeii.
Aoaioeiaai? iocaei? a??one iie?ii??e?oo, ye? i?ioyaii aeanyoe ?ie?a
i?ioiaeeee ianaae? a eoeueoo?ao ee?oei Vero i?e 37i, a?ae??ciyeeny
cia/ii iaioei ai?noii aa??aio?a Aaaio( a ii??aiyii? ?c aiaeia?/iei
iaai?ii ae?iaie/eo aaeoeeiieo ooai?a, ianaae? yeeo i?iaiaeeee i?e 34iN.
Oeie eoeueoo? ee?oei (Vero oa HEp-2) aeey ?ai?iaeoeoe?? a??on?a
i?aeoe/ii ia aieeaaee ia ai?no aeenioe?aio?a a iiioeyoe?yo.
Aeii?ioth/eie eiiiiiaioaie iiioeyoe?e o?ueio oei?a aoaioeiaaieo a??on?a
iie?ii??e?oo, iacaeaaeii a?ae aeaeo ee?oei, aoee a??one c aenieei
ao?i?oaoii aei aaioii?oo – Aaaio+. Naeaeoe?iiiaai? aaiaoe/i? aaioii?oia?
aa??aioe iiioeyoe?e aaeoeeiieo oa a??oeaioieo a??on?a iie?ii??e?oo
aeei?enoiaoaaee aeey iiaeaeueoiai ii??aiyeueiiai aiae?co ?o a?ieia?/ieo
aeanoeainoae.

Iae?ia??oeaioi?noue aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo. Cia/ia
oaaaa aei aea/aiiy iiioeyoe?eii? i?ieeaino? aioa?ia??on?a iaoiiaeaia
aeyaeaiiyi aaiaoe/ii? ianoaa?eueiino? aaeoeeiieo ooai?a a??on?a
iie?ii??e?oo (J. Drake et al., 1993; R.Duggal.et al., 1997). C iaoith
aaeaeaaoii? ioe?iee i?ioean?a i?ieeaino? iie?ia??on?a aoee noai?ai?
ia?ea?e iae?ia??oeaioiino?, ye? ia?aa??ythoue in vitro oa in vivo. ?nio?
ei?aeyoe?y i?ae iae?ia??oeaioi?noth a??on?a iie?ii??e?oo aeey ieoae oa
ethaeae (I.E.Ai?ioeeiaa,1979). ?aea? i?iaaaeaiiy iaaaaeaieo
aenia?eiaio?a aoee i?aeii?yaeeiaai?: 1-ii??aiyeueiiio aea/aiith
iae?ia??oeaioiino? aaiaoe/ieo aa??aio?a iiioeyoe?? Aaaio+ oa Aaaio( o
a??oeaioieo ooai?a a??on?a iie?ii??e?oo ?? oeio, 2- aecia/aiith aieeao
oiia eoeueoeaoaaiiy ia iiaeeea?noue aeieeiaiiy ?aaa?n??
iae?ia??oeaioiino? o aoaioeiaaieo iie?ia??on?a ?? oeio. Ioe?iea
iae?ia??oeaioiino? iaaaaeaia ca na?aaeiueith o?eaae?noth aeeooy oa
eaoaeuei?noth o ieoae (oaae.2).

Oaaeeoey 2

Iae?ia??oeaioi?noue aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo ?? oeio
MEF-1

A?oie ieoae (n=28),

?io?eiaaieo: Eaoaeuei?noue

a % Na?aaeiy o?eaae?noue aeeooy (aei?)

iiioeyoe??th iie?ia??on?a 96,24 ( 1,1 ?1(0,01

97,8 ( 0,3 ?2<0,02 4,4( 0,1 ?1(0,01 3,2 ( 0,3 ?2<0,01 aa??aioii Aaaio+ 98,8( 0,4 ?1(0,01 99,4 (0,5 ?2<0,05 3,1 ( 0,12 ?1(0,05 3,9 ( 0,39 ?2<0,01 aa??aioii Aaaio( 72,3 ( 0,6 ?1(0,01 84,7 ( 0,8 ?2<0,05 7,32 ( 0,78 ?1(0,01 5,91 ( 0,2 ?2<0,001 I?ei?oee: 1.*enaeueiee: ieo? NAA, ciaiaiiee: –BALB/c. 2.?1-??cieoey iae?ia??oeaioiino? aeey aeaio e?i?e ieoae 3. ?2 – ii??aiyiiy Aaaio+, Aaaio(, aeo?aeii? iiioeyoe??. Aiae?c a??oeaioiino?, i?iaaaeaiee ia ??ai? iiioeyoe?? ooaia a??ono iie?ii??e?oo ?? oeio MEF-1 iieacaa, ui aaeaioiaaiee aei ieoae oa aaaiaiyieo iaoethe?a ooai ni?e/eiya i?e ?io?aoea?aa?aeueiiio ?io?eoaaii? ee?i?/io ea?oeio iie?ii??e?oo c ?icaeoeii ia?ae?/?a e?ioe?aie. Aa??aio Aaaio+ ni?e/eiya eaoaeuei?noue o a?eueoi? e?eueeino? oaa?ei 98,8+0,4% i?ae aa??aio Aaaio(72,3+0,6%. Na?aaeiy o?eaae?noue aeeooy ieoae, ca?aaeaieo, aa??aioii Aaaio+ -3,1+0,12 aei?a aoea iaioa a 2,36 ?ace ca oaeo aeey Aaaio(- 7,32+0,78 aei?. I?e ?io?aoea?aa?aeueiiio aaaaeaii? aenieeo aeic “aa?y/eo” (o?eaae? eaai?aoi?i? ianaae? i?e 370N) aoaioeiaaieo a??on?a iie?ii??e?oo P712ch2ab, aeyaeaia ?o iae?ia??oeaioi?noue aeey ieoae, iniaeeai aeey aa??aioa iiioeyoe?? Aaaio+. ?nioaaiiy oaeeo ooai?a ia? aaaeeeaa ai?aeai?/ia cia/aiiy, iiaeiii aooe a?aoiaaiei i?e oaeineiiaeaii? iie?ii??e?oieo aaeoeei. A aiaeia?/ieo aeine?aeaeaiiyo c“oieiaeiaeie” (ianaae? i?e 340N) aa??aioaie iie?ia??on?a ?? oeio iae?ia??oeaioi?noue ia aoea aeyaeaia. Aioeaaii?noue a??on?a iie?ii??e?oo. Ioe?iea aioeaaiiino? a??on?a iie?ii??e?oo aeaiiino?o?, ui ca oe??th iciaeith a??oni? iiioeyoe?? aeineoue iaiaeii??aei? (oaae. 3). Oaaeeoey 3 Aioeaaii?noue aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo. Oeie oa ooaie a??on?a Oeo?e aioeo?e a log 2 (n=15) Aa??aio Aaaio+ Aa??aio Aaaio( A??oeaioi? a??one iie?ii??e?oo ? oei – Mahoney ?2<0,001 3,28(0,19 ?1(0,01 ?2<0,001;5,28(0,16; ?1(0,01 ?? oei – MEF-1 ?2<0,001 7,47(0,23 ?1(0,01 ?2<0,001;3,28(0,21; ?1(0,01 ??? oei – Saukett ?2<0,001 5,88(0,40 ?1(0,01 ?2<0,001;3,68(0,32; ?1(0,01 Aaeoeeii? a??one iie?ii??e?oo ? oei – LSC 2 ab ?2<0,001 4,47(0,45 ?1(0,001 ?2<0,001;1,28(i,40; ?1(0,02 ?? oei - P-712 ch 2ab ?2<0,001 7,47( 0,22 ?1(0,001 ?2<0,001;2,28(0,28; ?1(0,05 ??? oei Leon 12 ab ?2<0,001 5,28(0,38 ?1(0,001 ?2<0,001;2,47(0,18; ?1 (0,1 I?ei?oee: ?1 -??cieoey aioeaaiiino? i?ae oeiaie iie?ia??on?a , ?2 –??cieoey aioeaaiiino? i?ae aaiaoe/ieie aa??aioaie Aaaio+ oa Aaaio(). Iaea?eueo aenieith aioeaaii?noth a?ae??ciythoueny aa??aioe Aaaio+ aaeoeeiieo oa a??oeaioieo a??on?a iie?ii??e?oo ca aeiyoeii a??oeaioiiai ooaio ? oeio Mahoney, o yeiai aioeaaii?noue Aaaio( aoea aeuith a 1,6 ?ace aeuith, i?ae aeey aa??aioo Aaaio+. Aaee/eie oeo??a aioeo?e aei aaiaoe/ieo aa??aio?a Aaaio+ aoaioeiaaieo a??on?a iie?ii??e?oo aeu? i?ae aei aa??aio?a Aaaio( a 3,49; 3,27; 2,13 ?ace a?aeiia?aeii aeey ?, ??, ??? oei?a. Ia i?eeeaae? a??oeaioieo a??on?a iie?ii??e?oo aioeaaii?noue aaiaoe/ieo aa??aio?a Aaaio+ aoea oaeiae aeuith ca oaeo aeey aaiaoe/ieo aa??aio?a Aaaio( a 2,27 oa 1,59 ?ace a?aeiia?aeii aeey ??, ??? oei?a. Aa??aioe Aaaio+ iie?ia??on?a ?? oeio iaee iaeaeuee nooi?iue aioeaaiii? aeoeaiino?. ?ioi?iaoe?y i?i ??cio aioeaaii?noue aaiaoe/ieo aa??aio?a Aaaio+ oa Aaaio( aoaioeiaaieo iie?ia??on?a ia?niaeoeaia aeey oaeineiiaeaiiy AEIA oeyoii naeaeoe?? ?c aeo?aeieo iiioeyoe?e aenieiaioeaaiiiai aa??aioa Aaaio+. ?ioiiaaii?noue aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo ioe?ithaaee ca i?ioaeoeaiei aoaeoii ?ioi?caoe??, yea ia?aaeoaaea iiaeaeueoiio ca?aaeaiith iie?ii??e?oii. ?acoeueoaoe i?aaenoaaeai? a oaae. 4. NOAE oaa?ei ia oii? ?ioi?caoe?? aa??aioaie Aaaio+ oa Aaaio( ca?eueooaaeanue ii??aiyii c eiio?ieai a 2,45 oa 1,64 ?ace a?aeiia?aeii. Oaiaeaioe?th ia ei?enoue i?ioaeoeaiiai aoaeoo ?ioi?caoe?? aa??aioii Aaaio+ a?aei?/aee ii ciaioaiith eaoaeueiino? (41,6%) a ii??aiyii? c eiio?ieai (58,3%), ia oii? ?ioi?caoe?? aa??aioii Aaaio( eaoaeuei?noue aoea 50,1%. Oaaeeoey 4 ?ioiiaaiia (i?ioaeoeaia) ae?y aaiaoe/ieo aa??aio?a aaeoeeiiiai ooaio a??on?a iie?ii??e?oo ?? oeio i?e aenia?eiaioaeueiiio iie?ii??e?o? o ieoae. A?oie ieoae (n=28) Eaoaeuei?noue a % Na?aaeiy o?eaae?noue aeeooy (NOAE), aei? Ca?eueoaiiy NOAE a % Eiio?ieueia – ?ioaeoe?y iie?ia??onii ?? oeio MEF-1 58,3+0,9 10,1+0,9 Aea? ?ioi?caoe?? aa??aioii Aaaio+ aaeoeeiiiai iie?ia??ono ?? oeio 41,6+0,62 ?1(0,001 24,8+0,6 ?1(0,001 145,5 Aea? ?ioi?caoe?? aa??aioii Aaaio( aaeoeeiiiai iie?ia??ono ?? oeio 50,1+2,1 ?1(0,01 16,6+1,3 ?1(0,001 81,8 I?ei?oea: ?1- ??cieoey iieaciee?a i?ae eiio?ieueiith a?oiith oa a?oiaie, ?ioi?ciaaieie iie?ia??onaie aa??aio?a Aaaio+ oa Aaaio(. Aoea aeiaaaeaia ua iaeia nooo?aa ??cieoey a a?ieia?/ieo oa?aeoa?enoeeao aa??aio?a Aaaio+ oa Aaaio(iiioeyoe?e iie?ia??on?a. Aeniea ?ioiiaaii?noue aa??aioo iiioeyoe?? Aaaio+ iiaeiia aooe ei?eniith aeey i?ioeano oaeineiiaeaiiy aeeaeo iie?ii??e?oieo aaeoeei. ?ioiiiaoiaaiac aenia?eiaioaeueiiai iie?ii??e?oo ia ??ai? iiioeyoe?e iie?ia??on?a. Aeey aea/aiiy iaoiaaiaco oa ?ioi?oaoo i?e aioa?ia??onieo ?ioaeoe?yo, iae/ano?oa aeei?enoiaothoue ?io?aoea?aa?aeueia, ?io?anieiaeueia ca?aaeaiiy iaai (I.I.?aeueo, 1982). A??oeaioi? a??one iie?ii??e?oo ?? oeio aoee aaeaioiaai? aei iiaiia?iaeaeaieo aaaiaiyieo iaoethe?a oa a?eeo ieoae. ?ai?iaeoeoe?y iie?ia??on?a a?aeaoaa?oueny a iiceo oaa?ei, i’ycao, na?oe?, ie?eao, ia/?ioe?, niinoa??aathoue a??onai?th (E.A.?iaeicii,1975). A i?aai?ci? ethaeeie i?ae /an ?ai?iaeoeoe?? aioa?ia??on?a iino?eii a?aeaoaa?oueny aeenioe?aoe?y, aeenioe?aioe iiioeyoe?e ni?e/eiythoue niaeo? ??ciiiai?oieo iaoiaaiaoe/ieo yaeu, ci?iththoue eeane/i? eaiiie uiaei ee?i?/ieo i?iya?a ?ioaeoe??, aieeaathoue ia aoaeoeai?noue iaoiae?a i?io?eaeoeee, ae?aaiinoeee aioa?ia??onieo ?ioaeoe?e. Aoee noai?ai? iiaeae? iie?ii??e?oii? ?ioaeoe??: i?ney ?io?aoea?aa?aeueiiai ca?aaeaiiy ieoae aa??aioaie Aaaio+ oa Aaaio( i?iaiaeeee niinoa?aaeaiiy ca ?icaeoeii ee?i?/ieo neiioii?a caoai?thaaiiy - neaaeino?, o?aio?iiy, ?icaeoeo ia?ae?/?a e?ioe?aie oao?aieaa??, /a?ac 1-3 aeiae ieo? aie?aee (oaae.5). Aeyaeaia ia?aeiieeaa ??cieoey i?ae aaee/eiaie ?io?eoth/eo aeic aeey aa??aioo Aaaio+ - 240,38 AOI, oa Aaaio(- aeica aoea a 30,14 ?ace a?eueoith- 7246,37 AOI. NOAE a iiaeae? i?e ?io?eoaaii? aa??aioii Aaaio( aoea ia 5,5 aeiy a?eueoa, i?ae i?e ?io?eoaaii? aa??aioii Aaaio+, ui aeiaiaeeoue aeuo a??oeaioi?noue o Aaaio+. Oaaeeoey 5 Noai?aiiy iiaeaeae iie?ii??e?oii? ?ioaeoe?? c 50% eaoaeuei?noth o ieoae i?e ?ioaeoe?? aaiaoe/ieie aa??aioaie a??on?a iie?ii??e?oo ?? oeio MEF-1. Ieo? ?io?-eiaai? (n=114) Eoioeyoeaia eaoaeuei?noue a % Na?aaeiy o?eaa-e?noue aeeooy (aei?) ?io?eoth/a aeica aeey 50% eaoaeueiino? (a AOI) Lg LD50 aeo?aeiith iiioeyoe??th 64,2+1,2 10,4+0,4 347,22.(11,3 4,16 aa??aioii Aaaio+ 71,4+0,7 ?1, ?2(0,01 8,4+0,5 ?1, ?2(0,001 240,38.(6,9 ?1, ?2(0,001 4,32 aa??aioii Aaaio( 50,0+0,9 ?1, ?2(0,001 13,9+0,2 ?1, ?2(0,001 7246,37.(17,5 ?1, ?2(0,001 2,84 I?ei?oea: ?1 -??cieoey i?ae iiioeyoe??th oa aa??aioaie Aaaio+ , Aaaio(; ?2- i?ae aa??aioaie. ?ai?iaeoeoe?y aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo a i?aai?ci? ieoae A?aeii? iiaeae? aenia?eiaioaeueiiai iie?ii??e?oo, a yeeo aea/aee ?iciiae?e a??oeaioieo ooai?a a??on?a iie?ii??e?oo?a a aieiaiiio iiceo, nieiiiio iiceo, na?oe?, i’ycao (I.A. Ianyiieeiaa, 1970; E.A. Ooeaeeia, 1975). Aoei aaaeeeai aeine?aeeoe aca?iiae?th a??on?a iie?ii??e?oo oa ?o aaiaoe/ieo aa??aio?a c i?aaiaie ?ioiii? nenoaie: oeionii oa naeac?ieith. ?ioi?iaoe?y i?i aeeiai?eo ?ai?iaeoeoe?? aa??aio?a iiioeyoe?? iie?ia??on?a a i?aaiao ?ioiii? nenoaie oa na?oe? o ieoae iaaaaeaia a oaae.6. Oaaeeoey 6 Aeeiai?ea ?ai?iaeoeoe?? aaiaoe/ieo aa??aio?a a??oeaioieo a??on?a iie?ii??e?oo ooaio MEF-1 a i?aai?ci? ieoae (n=40). I?aaie Aa??aioe Oa?i?ie niinoa?aaeaiiy 12 aiaeei 24 aiaeeie 48 aiaeei 72 aiaeeie Na?oea Aaaio+ (8,1(0,04)o102 (1,7(0.11)o103 (5,7(0,04)o101 (2,9(0,03)o101 Aaaio( (5,3(0,02)o102 (9,1(0,01)o102 (3,9(0,02)o102 0 Oeion Aaaio+ (6,3(0,04)o103 (4,1(0,12)o103 (9,6(0,02)o101 0 Aaaio( (8,8(0,03)o103 (7,2(0,04)o104 (4,3(0,005)o102 0 Naeac?iea Aaaio+ (8,6(0,01)o102 (3,7(0,06)o103 (3,9(0,04)o102 (7,9(0,02)o101 Aaaio( (6,6(0,04)o102 (9,1(0,12)o102 (5,7(0,02)o103 (3,8(0,07)o102 I?ei?oee:1. Oeeo?aie a oaaeeoe? i?aaenoaaeaia e?euee?noue a??on?a a AOI ia 1 a oeaieie. 2. p1<0,001, aeinoia??ia ??cieoey ai?noo a i?aaiao aa??aio?a Aaaio+ oa Aaaio(. 3. p2<0,001- ??cieoey ai?noo aa??aio?a Aaaio+ oa Aaaio( a oeion? oa naeac?ioe? a ii??aiyii? c na?oeai. Aaaaeaee, ui eiioeaio?aoe?y a??on?a a na?oe? oaa?ei ei?aeth? c oaeith a e?ia?, oiaoi iiaea aooe iieacieeii a??onai??. I?e ca?aaeaii? aa??aioii Aaaio+ aaea /a?ac 12 aiaeei a??one aeae?eyee ?c na?oey a oeo?? 8,1+0,04o102 AOI/a, ia 24 aiaeeio ?o e?euee?noue ca?eueooaaeanue aei 1,7+0,11o103 AOI/a, ia 72 aiaeeio ciaiooaaeanue aei2,9+0,03o101. A??one aa??aioo Aaaio( aeae?eyee ?c na?oey c 12-? aei 48 aiaeeie. A??one c oa?aeoa?enoeeaie Aaaio+ oa Aaaio( aeae?eythoueny ?c oeiono c 12-? aei 48-? aiaeeie, aea e?euee?noue Aaaio( aoea ia iaeei lg aeua. Iniaeeai iii?oiith aoea ??cieoey a eiioeaio?aoe?? a??on?a ia 24-oo aiaeeio: ai?no aa??aioo Aaaio(? cia/iei a oeion?, a?i a?eueoa a 17,5 ?ac i?ae aeey aa??aioa Aaaio+, aea a naeac?ioe? e?euee?noue Aaaio+ a 4,06 ?ace a?eueoa ca ai?no a??on?a aa??aioo Aaaio(.Iaeaeaa aa??aioe a?eueo o?eaaei ciaoiaeeeenue a naeac?ioe?, i?ae a oeion?. ?ioi?iaoe?y i?i nai???aeiee o?ii?ci iiioeyoe?? Aaaio(, aei i?aai?a ?ioiiiai caoenoo, i?i iniaeeaino? ?iciiae?eo aaiaoe/ieo aa??aio?a iie?ia??on?a ?? oeio MEF-1 a oeion? oa naeac?ioe? i?e iie?ii??e?oi?e ?ioaeoe?? ? aaniethoii iiaith. Aiane?aeie o?aiceoi?ii? ?ioaeoe?? ?ioiieiiiaoaioieo i?aai?a, a??ia?aeii ci?ith?oueny ?ioiia a?aeiia?aeue aei aaoa?ieia?/ieo aioeaai?a oa iiaea aeieeaoe ?ioiinoi?an?y. Oeueo?ano?oeoo?i? ci?ie a i?aaiao ?ioiii? nenoaie, iaoiiaeai? aaiaoe/ieie aa??aioaie iie?ia??on?a. I?e aeaeo?iiii-i?e?ineii?/iiio aeine?aeaeaii? no?oeoo?e ?ioiieiiiaoaioieo ee?oei. a ia?oo aiaeeio ?ioaeoe?? aeyaeyee a??oni? /anoi/ee aa??aio?a Aaaio+ a aiaeioeeoicieo aaeoieyo oeeoiieacie naeac?iee oa oeiono. A a?eueoino? ?ioiieiiiaoaioieo ee?oei aeoea?coaaeany a?eie-i?iaeoeoth/a ae?yeuei?noue, niinoa??aaee ca?eueoaiiy ?ici???a yaea?aoeue, yaea?ieo ? oeeoiieaciaoe/ieo a?aioe ?II. Aa??aio Aaaio+ i?aeneethaaa aeoeai?noue iae?ioaa?a: ca?eueoaiiy ?o /enea, ?ici???a oa e?ciniiiiiae?aieo ooai?aiue. I?e ae?? aa??ioo Aaaio( a???iie aeyaeyee a iniiaiiio a oeeoiieaci?, a naeac?ioe?, ii?o/ c i?aeaeuaiiyi iae?ioaaaeueii? aeoeaiino?, aoee aeano?oeoeaii ci?iai? ?ioiieiiiaoaioi? ee?oeie.*a?ac 6 aiaeei ?ioaeoe?? aa??aioii Aaaio( c?inoaea e?euee?noue aiiioicieo ee?oei, ye? cii?uoaaeeny, ?o yae?a i?eiioe/ii ci?ithaaeenue, ca?eueooaaeanue e?euee?noue eiiaeainiaaiiai, ia?a?iaeueii ?ici?uaiiai o?iiaoeio. Ia`?i aoo?iiaoeio aoa cia/ii iaioee ia ?aii?o noaae?yo aiiioico, a ia i?ci?o- iiai?noth a?aenooi?e, ? yae?i caciaaaei ea??i?aeneno. A oeeoiieaci? a?aei?/aee e?cen iao?eeno , iaa?ye i?oioiiae??e oa aeano?oeoe?th ?ioeo i?aaiae. Oae? ee?oeie oaaioeeooaaeeny iae?ioaaaie aai ?ioa?aeea?ooth/eie oa aeaiae?eoieie ee?oeiaie. E?euee?noue oeeoie?oe/ieo i?iya?a, ni?e/eiaieo a??onaie a ?ioiieiiiaoaioieo ee?oeiao, aoea caeiiii??ii aeua i?e ae?? aa??aioo Aaaio(, oe? a??one ?iaeoeoaaee aiiioic- cai?ia?aiiaaio, a ee?oei? nia?oue. A??onaie – ?iaeoeoi?aie aiiioico iiaeooue aooe a??on ?ioiiaeao?oeeoo ethaeeie, a?eio oa ?io? (D.L. Vau?, G. Hacker, 1995; S.Nagata, 1996), aea a?aeiiino?. i?i iiaeeeaino? ee?oeiiiai aiiioiciiai oeyoo i?e aioa?ia??oni?e ?ioaeoe?? a?aenooi?. Aieea aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo ia oi?ioaaiiy aoii?aeueiiai oa ee?oeiiiai ?ioi?oaoo. I?ioean ?ioiiaaiaco i?e iie?ii??e?o? yaey? ooai?aiiy ne?iaaoeiaeo oa nae?aoi?ieo aioeo?e, ?icaeoie i?noeaai? ?acenoaioiino? oeaiei, ae?th oaeoi??a ee?oeiiiai ?ioi?oaoo. I?iaeiaaeo?oueny aeeneon?y i?i aeii?ioth/o ?ieue aoii?aeueieo aai ee?oeiieo eiiiiiaio?a ?ioiii? a?aeiia?ae? i?e ?ioaeoe?? aai aaeoeeiaoe?? (E. Ehrenfeld et al., 1995; J. Modlin, 1995). Aaiaoe/ia iaiaeii??aei?noue iiioeyoe?e aaeoeeiieo oa a??oeaioieo a??on?a iie?ii??e?oo, ?nioaaiiy aa??aio?a Aaaio+ oa Aaaio( c ??ciith a??oeaioi?noth, aioeaaii?noth, ?ioiiaaii?noth, na?ae/eoue i?i iiaeeeao ??cieoeth a i?ioean? ?aae?caoe?? ?ioiii? a?aeiia?ae? ia oe? a??one. Aeyaeee, ui a??oniaeo?ae?coth/? aioeo?ea c”yaeythoueny a ne?iaaooe? e?ia? c 3-ai aeiy niinoa?aaeaiue, ?o aaee/eie aeinoia??ii a?eueo? aeey aa??aioo Aaaio+, i?ae Aaaio(. Iaeneiaeuei? oeo?e aioeo?e niinoa??aaee ia 7 aeaiue – 7,47+0,11; 7,47+0,19 – aeey aa??aio?a Aaaio+ a??oeaioiiai oa aaeoeeiiiai ooai?a. Aeey aa??aioo Aaaio(- a??oeaioiiai ooaio iaeneiaeueiee oeo?– 3,28+0,13 oaeiae iia’ycaiee ?c 7-i aeiai ?ioi?caoe??, a i?e ?ioi?caoe?? Aaaio( aaeoeeiiiai ooaio – i?e aioeo?eiooai?aiiy 3,08+0,19 a?aei?/aee aaea ia 5 aeaiue. I?ney 7-ai aeiy niinoa?aaeaiue oeo?e aioeo?e aei an?o aa??aio?a iinooiiai cieaeothoueny. Ca nooiaiai aioeaaiii? aeoeaiino? aaeoeeiiee ooai a??ono iie?ii??e?oo ?? oeio P712ch2ab ia iinooia?oueny eiai a??oeaioiiio aiaeiao– ooaio MEF-1 Aa??aioe Aaaio+ iaio a??on?a aoee a?eueo aioeaaiieie, i?ae Aaaio(. Aeoeaaoe?th O-e?ioioeeo?a i?ae ae??th i?oiaaio OAA, ioe?ithaaee ca ?ioaineai?noth aeeth/aiiy 3I-oei?aeeio a AeIE ee?oei, ui ae?eyoueny. Oaeee aoaeo niinoa??aaee c 3 aeiae aeey an?o aa??aio?a aenia?eiaio?a i?e ii??aiyii? eiio?ieueieo iieaciee?a – 528+132 ?c aeine?aeieie– 4528+258, 3910+116 – aeey aa??aio?a Aaaio+; oa 2312+29, 1348+78 – aeey a?aeiia?aeieo aa??aio?a Aaaio(. Aeoeaaoe?y O-e?ioioeeo?a, ?o aeniea i?ie?oa?aoeaia caeaoi?noue, ?iaeoeiaaia OAA, ciaiooaaeanue aei 5 aeiae. Noeioeyoe?y ooieoe?iiaeueii? aeoeaiino? A-e?ioioeeo?a i?ae ae??th i?oiaaia EIN aoea iaaecae/aeii ?ioaineaiith oa aeey Aaaio+ aeuith, i?ae aeey Aaaio(, ui i?aeoaa?aeaeo? ia?aaaaeio aeoeai?noue aa??aioa Aaaio+. I?oiaaiia ae?y a??oniiai O-caeaaeiiai aioeaaio a eoeueoo?? e?ioioeeo?a naeac?iee aoea aeecueeith aei oaei? o OAA. I?ie?oa?aoeaia a?aeiia?aeue e?ioioeeo?a ia a??oniee aioeaai iieacaea, ui ooieoe?iiaeueia aeoeai?noue e?ioioeeo?a iaea a??on-caeaaeiee oa?aeoa?. Ii??aiyeueiee aiae?c i?ioaeoeaieo iaoai?ci?a ?ioi?oaoo i?e aenia?eiaioaeueiiio iie?ii??e?o?. I?ioea??oni? aioeo?ea iiaeooue iaoe ooeiaeaeoth/o ae?th, iia’ycaio c a?aeeeaaeaiiyi a noaeeiao ?ioiieo eiiieaen?a. Nainea?e?ciaai? e?ioioeeoe caeaoi? ni?e/eiyoe oeeoioiene/i? aoaeoe, ?iciianthaeaeaieie ?ioiiiaoieia?/ieie ?aaeoe?yie i?e a??onieo ?ioaeoe?yo ? aooi?ioii? ?aaeoe??, oai?iae ?ioiieo eiiieaen?a. (M. Bendinelli, 1984) A oiiaao aenia?eiaioaeueii? iie?ia??onii? ?ioaeoe?? ? iiaeeeaino? aeey ii??aiyeueiiai aiae?co i?ioaeoeaieo iaoai?ci?a ?ioi?oaoo (oaae.7). A a?oi? ?aoeei??io?a aeae?eyee 2 i?aea?oie. 1.1– ieoae ?io?eoaaee ?io?aoea?aa?aeueii a??onii iie?ii??e?oo ?? oeio, a??oeaioiei ooaiii MEF-1 a aeic?, 500 AOI (LD50 ). 1.2 – ieoai aaiaeeee ca 48 aiaeei aei iiaeaethaaiiy iie?ii??e?oii? ?ioaeoe?? i?aia?ao oeeeeioinoai 2 ia/ea (ye ?ioiiaeai?anaio, ui i?eai?/o? i?ie?oa?aoe?th e?ioioeeoa?ieo eeii?a). I?ney ca?aaeaiiy ieoai aioo??oiueiaaiii oa aioo??oiuei/a?aaeiii aaiaeeee ??ci? caniae ?ioiioa?ai?? a?ae ieoae-aeiii??a. I?e aino??e iie?ii??e?oi?e ?ioaeoe?? c ei?ioeei ia?aa?aii, (NOAE ieoae – 3,84+0,41 aeiy) i?ioaeoeaiee aoaeo aeyaeee oae? oaeoi?e, ye ianeaia ?ioi?caoe?y, iniaeeai ne?iaaoee c niaoeeo?/ieie i?ioe a??oeaioiiai ooaio iie?ia??ono aioeo?eaie. NOAE o ieoae ca?eueoo?oueny ia 77%. Oaaeeoey 7 I?ioaeoeaia ae?y oaeoi??a ?ioiiiai caoenoo i?e aenia?eiaioaeueiiio iie?ii??e?o? Aeey ?ioiioa?ai?? aeei?enoai?: Ieo? ?aoeei??ioe, ca?aaeai? iie?ia??onii MEF-1 (a?oia 1.1) (n=70) Ieo? ?aoeei??ioe, c i?eai?/aiei ?ioi?oaoii, ca?aaeai? iie?ia??onii MEF-1 (a?oia 1.2) (n=70) Na?aaeiy o?eaae?noue aeeooy (NOAE), aei? Ca?eueoaiiy NOAE a % NOAE, aei? Ca?eueoaiiy NOAE a % ne?iaaoee i?ioe aaeoeeiiiai iie?ia??ono 6,1+0,21 p<0,001 58,85+1,2 4,0+0,32 ia aeyaeaii ne?iaaoee i?ioe a??oeaioiiai iie?ia??ono 6,8+0,49 p<0,01 77,0+0,9 4,4+0,73 ia aeyaeaii nieaiioeeoe ia?ioi?ciaaieo ieoae 3,91+0,27 p(0,1 ia aeyaeaii 4,23+0,85 ia aeyaeaii nieaiioeeoe ?ioi?ciaaieo ieoae 4,08+0,41 p(0,1 ia aeyaeaii 4,13+1,03 ia aeyaeaii nieaiioeeoe ia?ioi?ciaaieo ieoae oa aioeo?ea i?ioe aaeoeeiiiai iie?ia??ono 6,3+0,14 p<0,001 64,06+1,5 6,6+0,36 p<0,02 51,37+0,4 nieaiioeeoe ia?ioi?ciaaieo ieoae oa aioeo?ea i?ioe a??oeaioiiai iie?ia??ono 7,0+0,22 p<0,001 82,29+1,9 6,5+0,4 p<0,02 49,08+1,6 eiio?ieue - iie?ia??onia ?ioaeoe?y NOAE- 3,84+0,41 NOAE- 4,36+0,74 I?ei?oea: p-??cieoey iieaciee?a a aeine?aeieo oa eiio?ieueieo a?oiao Ne?iaaoea c aioeo?eaie i?ioe aaeoeeiiiai ooaio iaoiiaeth? aoaeo caoenoo ieoae ia 58,85%. I?e eiia?iaoe?? ?ioiieo aioeo?e c ianainea?e?ciaaieie nieaiioeeoaie ?ioiioa?ai?y aoea oaeiae iioeiaeueiith, NOAE ca?eueooaaeanue ia 82,29%, a?aeiinii eiio?ieth. Aa??aioe ?ioiioa?ai??, iia’ycai? c ia?aiinii nainea?e?ciaaieo aai ianainea?e?ciaaieo e?ioioeeo?a ia aoee aoaeoeaieie. O ieoae ?c i?eai?/aiei oeeeeioinoaiii ?ioi?oaoii i?ioea??oni? aioeo?ea, ?ioii? oa ia?ioii? nieaiioeeoe, ia i?ecaiaeeee aei i?ioaeoeaiiai aoaeoo. I?iaeiaaeaiiy aeeooy ieoae aoei aeinyaiooi i?e canoinoaaii? eiiiiceoe?? ?c ?ioiieo ne?iaaoie oa ianainea?e?ciaaieo nieaiioeeo?a. A oiiaao noai?aii? iiaeae? iie?ii??e?oii? ?ioaeoe?? niinoa??aaee neeaaei?, iaiaeiicia/i? ca o?aeooaaiiyi, caoeni? aa??aioe ?ioiioa?ai??. O ieoae c ii?iaeueiith ?ioiiith nenoaiith i?ioaeoeaia ae?y aoea iia’ycaia c a??oniaeo?ae?coth/ith aeoeai?noth aioeo?e ne?iaaoee, iniaeeai aiiieia?/ieo. O ?ioiiaeao?oeeoieo oaa?ei ?ioiioa?ai?y iie?ii??e?oii? ?ioaeoe?? aoea oni?oiith i?e aeei?enoaii? ia?ioiieo nieaiioeeo?a a eiiieaen? c ne?iaaoeiaeie aioeo?eaie. A??one iie?ii??e?oo ye ?ioiiiiaeoeyoi?e. ?ioiiiiaeoethth/? aoaeoe, iia’ycai? c aoaioeiaaieie aaeoeeiieie ooaiaie a??on?a iie?ii??e?oo, i?aeoe/ii ia aea/ai?. Caaaeath/e ia ianiaa canoinoaaiiy aeeai? iie?ii??e?oii? aaeoeeie, ae?ae iaiao?aeii aea/eoe ci?ie ?ioiieia?/ii? ?aaeoeaiino? , iaoiiaeai? aaeoeeiaoe??th i?ioe iie?ii??e?oo. Oea ?aeaeii iaoiiaeei i?iaaaeaiiy aeine?aeaeaiue ?icae?eo, i?enay/aieo aea/aiith aieeao a??oeaioieo oa aaeoeeiieo iie?ia??on?a ? ?o aaiaoe/ieo aa??aio?a ia aoii?aeueio oa ee?oeiio ?ioiio a?aeiia?aeue aei aaoa?ieia?/ieo aioeaai?a. ?ioiiiiaeoethth/a ae?y a??on?a iie?ii??e?oo a?aeiinii aioeo?eiooai?aiiy aei aaoa?ieia?/ieo aioeaai?a - a?eo?ioeeo?a aa?aia (oaae.8). Oaaeeoey 8 ?ioiiiiaeoeyoe?y aioeo?eiooai?aiiy aei aaoa?ieia?/ieo aioeaai?a iie?ia??onaie A?oie oaa?ei, n=15 Aa??aioe ?ioi?caoe?? Aaeoeeii? iie?ia??one A??oeaioi? iie?ia??one AOE ia 1o106 nieaiioeeo?a Ciaioaiiy AOE a % AOE ia 1o106 nieaiioeeo?a Ciaioaiiy AOE a % iie?ia??onii ? oeio, aa??aioii Aaaio+ 62,8+2,9 p1<0,001 33,34+0,7 39,5+0,9 p1<0,001 58,07+1,3 iie?ia??onii ? oeio, aa??aioii Aaaio( 51,3+2,7 p2<0,02 45,6+1,1 34,6+1,2 p2<0,01 63,27+0,8 iie?ia??onii ?? oeio, aa??aioii Aaaio+ 55,8+3,2 p1<0,001 40,8+0,4 45,4+3,1 p1<0,001 51,81+0,6 iie?ia??onii ?? oeio, aa??aioii Aaaio( 44,2+2,1 p2<0,02 53,08+2,2 38,6+2,4 p2<0,01 59,03+0,9 iie?ia??onii ???oeio, aa??aioii Aaaio+ 33,7+1,3 p1<0,001 64,23+1,5 43,9+1,8 ?1<0,001 64,02+2,1 iie?ia??onii ???oeio, aa??aioii Aaaio( 26,8+2,5 p2<0,02 71,55+0,9 37,6+2,3 ?2<0,001 60,09+0,3 AEIA 33,4+1,7 p1<0,001 64,55+1,6 94,2+2,3 Eiio?ieueia a?oia 94,2+2,3 I?ei?oee: 1.AOE- /enei aioeo?eiooai?thth/eo ee?oei 2.- ?1- ??cieoey o?ueio oei?a iie?ia??on?a a?aeiinii eiio?ieth; ?2 -??cieoey i?ae aa??aioaie Aaaio+ oa Aaaio(. Aeyaeaii,ui aaeoeeii? ooaie a??on?a iie?ii??e?oo oa ?o aaiaoe/i? aa??aioe i?iyaeyee ae?aaeaio ?ioiinoi?aneaio ae?th: i?eai?/oaaee aioeo?eiooai?aiiy aei oeioncaeaaeieo aaoa?ieia?/ieo a?eo?ioeeo?a. ?ioiiiiaeoethth/? aoaeoe, ye? a?aeoai?thaaeeny, aecia/aeeny oeiiaith oa?aeoa?enoeeith a??on?a oa caeaaeaee a?ae aaiaoe/iiai aa??aioo. Iaea?eueoei ?ioiiaeai?aneaiei aoaeoii a?ae??ciyany aoaioeiaaiee iie?ia??on ??? oeio. I?e ii??aiyii? ?ioiiiiaeoethth/eo aeanoeainoae aaiaoe/ieo aa??aio?a a??on?a iie?ii??e?oo i/aaeaeiith ? oaea oaiaeaioe?y: aaiaoe/i? aa??aioe Aaaio( cia/ii a?eueoa i?eai?/othoue ooai?aiiy aioeo?e a ii??aiyii? c aieeaii aa??aio?a Aaaio+. Iniaeeaee aoaeo ?ioiinoi?an??, ciaioaiiy e?eueeino? AOE aei a?eo?ioeeo?a aa?aia ia 71,55%, ?icaeaaany ia oii? ?ioi?caoe?? aa??aioii Aaaio( aoaioeiaaiiai a??ono iie?ii??e?oo ??? oeio, aea aeey ?ioeo aeaio oei?a iie?ia??on?a oaeiae niinoa??aaee aiaeia?/io oaiaeaioe?th a?eueo ae?acii? ?ioiinoi?aneaii? ae?? iaoiiaeaii? aieeaii aa??aio?a iiioeyoe?? Aaaio(. Aeey anioe?aoe?? iie?ia??on?a o aeaeyae? aeeai? iie?ii??e?oii? aaeoeeie oaeiae aoa aeyaeaiee ?ioiinoi?aneaiee aoaeo - cieaeaiiy e?eueeino? AOE a?aeiinii eiio?ieth ia 64,55%. Aaiaoe/i? aa??aioe Aaaio+ iie?ia??on?a iathoue a?eueo aenieo aioeaaii?noue oa ?ioiiaaii?noue, i?ae aa??aioe Aaaio(. Ae?acia ?ioiinoi?aneaia ae?y inoaii?o ia iia’ycaia c oeeie iciaeaie. Ii??aiyiiy ?ioiiiiaeoethth/eo aoaeo?a, ni?e/eiaieo aaeoeeiieie oa a??oeaioieie iie?ia??onaie aeiaaei, ui ?ioiiiiaeoethth/? aoaeoe i?eoaiaii? ye aaeoeeiiei, oae ? a??oeaioiei iie?ia??onai, oiaoi “Aoaioaoe?y ia a?aei?iy? ?ioiiiiaeoeyoe??”(A. O. Naiaiia, 1981). Iiaeia aoei iia’ycaoe ae?aci? ?ioiiiiaeoethth/? aoaeoe ?? oa ??? oeio aaeoeeiieo oa a??oeaioieo iie?ia??on?a c a??oeaioi?noth. Eiio?a?aoiaioii ? oa, ui a on?o aoaioeiaaieo ooaiao a?eueo ?ioiinoi?aneaiei aoa aaiaoe/iee aa??aio Aaaio(, a a?i ia? iaioo a??oeaioi?noue, i?ae Aaaio+. ?ioiiiiaeoethth/o ae?th a??on?a iie?ii??e?oo a?aeiinii ee?oeiieo ?aaeoe?e ?ioi?oaoo aei aaoa?ieia?/ieo aioeaai?a (oeiioeeo?a iaoethea) aea/aee a I*NO, eeane/i?e aea?a?/i?e ?aaeoe??, yea a?aeca?eaeth? noai ee?oeiiiai ?ioi?oaoo. Iieacaii, ui a noai?ai?e iiaeae? an? oeie iie?ia??on?a ni?e/eiyee cia/ia i?eai?/aiiy I*NO. Iaeaeuee i?iya ?ioiinoi?an?? aoa iia’ycaiee ?c iie?ia??onaie ??? oeio, aaeoeeii? ooaie oa aaiaoe/i? aa??aioe Aaaio( aoee a?eueo aeoeaieie a?aeiinii i?eai?/aiiy I*NO, i?ae aa??aioe Aaaio+(oaae.9). ?ioi?caoe?y AEIA ni?e/eiyea nooo?aee ?ioiinoi?aneaiee aoaeo, i?iyae I*NO ciaiooaaeenue ia 62,27%. ?iaeoeiaaia iie?ia??onaie oa ?o aaiaoe/ieie aa??aioaie ?ioiinoi?an?y aoii?aeueieo oa ee?oeiieo ?aaeoe?e ?ioi?oaoo ia aaoa?iaioeaaie aeeeeea? na?eiciee ?ioa?an c oi/ee ci?o iaoiaaiaco, ?ioiiaaiaco oa i?io?eaeoeee aioa?ia??onieo ?ioaeoe?e. Caeaoi?noue aaeoeeiieo ooai?a a??on?a iie?ii??e?oo aieeaaoe ia oi?ioaaiiy ?ioiii? a?aeiia?ae? aei ?ioeo aioeaai?a caeiiii??ii i?eaaee aei ianooiieo aoai?a aea/aiiy iie?ia??on?a ye ?ioiiiiaeoeyoi??a. Aoei aeioe?eueii ia?aianoe aea/aiiy aoaioeiaaieo ooai?a iie?ia??on?a ?, ??, ??? oei?a ia ??aaiue aeeai? iie?ii??e?oii? aaeoeeie, yea yaey? niaith anioe?aoe?th a??on?a iie?ii??e?oo, aoaioeiaaieo Naa?iei. Na?aae ??ciiiai?oieo i?iya?a a?ieia?/ii? aeoeaiino? AEIA eia?/ii aea/eoe ?ioiiiiaeoethth/? aoaeoe. I?e aea/aii? oaiiiaio a??on?iaeoeiaaii? ?ioiiiiaeoeyoe?? a ?ie? aaoa?ieia?/ieo aioeaai?a iiaeooue aooe iaoiaaii? oa iaiaoiaaii? aaeoa???, a??one, eiiiiiaioe aaeoeei. Naia oiio a iiaeaeueoeo aeine?aeaeaiiyo aoee noai?ai? iia? iiaeae? ioe?iee ?ioiiiiaeoethth/i? ae?? a??on?a iie?ii??e?oo ia i?eeeaae? AEIA. Oaaeeoey 9 Aieea aaeoeeiieo ooai?a a??on?a iie?ii??e?oo ia i?iya ?aaeoe?? I*NO o ieoae a?aeiinii aaoa?ieia?/ieo aioeaai?a (oeiioeeo?a iaoethea). A?oie oaa?ei, n=15. Aa??aioe ?ioi?caoe?? Aaeoeeii? ooaie iie?ia??on?a A??oeaioi? ooaie iie?ia??on?a ??cieoey iane e?ioiaoce?a, aaniethoia a % Ciaioai-iy i?iya?a I*NO a % ??cieoey iane e?ioiaoce?a, aaniethoia a % Ciaioaiiy i?iya?a I*NO a % iie?ia??onii ? oeio, aa??aioii Aaaio+ 0,92 (0,11 57,86 p<0,05 42,14(0,73 0,79(0,14 49,68 p<0,02 50,32(1,1 iie?ia??onii ? oeio, aa??aioii Aaaio( 0,26(0,08 16,35 p<0,001 83,65(0,89 0,24(0,08 15,09 p<0,001 84,91(0,88 iie?ia??onii ?? oeio, aa??aioii Aaaio+ 1,03(0,04 44,6 p<0,05 55,4(1,2 0,82(0,15 51,57 p<0,05 48,43(0,45 iie?ia??onii ?? oeio, aa??aioii Aaaio( 0,63(0,07 39,62 p<0,01 60,38(0,43 0,4(0,03 25,15 p<0,001 74,85(0,6 iie?ia??onii ???oeio, aa??aioii Aaaio+ 0,91(0,08 57,2 p<0,05 42,8(0,8 0,54(0,19 33,96 p<0,01 66,04(0,84 iie?ia??onii ???oeio, aa??aioii Aaaio( 0,27(0,06 16,9 p<0,001 83,1(1,6 0,27(0,05 16,98 p<0,001 83,02(1,22 AEIA 0,60(0,04 37,73 p<0,01 62,27(1,5 1,59(0,23 100 Eiio?ieueia a?oia 1,59(0,23 100 I?ei?oea: ? -??cieoey iieaciee?a i?ae aeine?aeieie oa eiio?ieueiith a?oiaie, a oaeiae i?ae ae??th aa??aio?a Aaaio+ oa Aaaio( o?ueio oei?a iie?ia??on?a Aieea aeeai? iie?ii??e?oii? aaeoeeie ia ia?aa?a noao?eieieiai? ?ioaeoe?? Noao?eieieiaa ?ioaeoe?y o ieoae iaea ae?aci? ee?i?/i? iciaee aac eaoaeueiiai e?ioey, ?icaeaaeanue iaaecae/aeii ?ioaineaii, aaeoa???i?y aeieeaea a ia?oo aiaeeio niinoa?aaeaiiy. C oeueiai /ano aecia/aee aeinoia??io ??cieoeth i?ae e?euee?noth aaeoa??e a e?ia? a eiio?ieueieo a?oiao oa i?ae aieeaii ?ioi?caoe?? AEIA- ciaioaiiy /enea noao?eieie?a a 5,47 ?ac. Iaea?eueo ?noioiee aieea ?ioi?caoe?? AEIA niinoa??aaee i?e aeae?eaii? aaeoa??e ?c naeac?iee: ciaioaiiy ian?i”yi?iiy noao?eieieaie aoei a 12,16 ?ace. Aa?”??i? oa aioei?e?iai? ooieoe?? e?iaioai?ieo oa ?ioiieiiiaoaioieo i?aai?a iaoiiaeththoue aenieee nooi?iue iaeiie/aiiy a ieo aaeoa??e, aea ia nueiio aeiao, i?e ?ioi?caoe?? AEIA, ia?aaaaeaee i?ioeane ae?i?iaoe?? noao?eieie?a c e?ia?, eaaai?a oa ia/?iee ia oii? cia/ii? eiioeaio?aoe?? aaeoa??e o ieoae eiio?ieueii? a?oie. Oaeei /eiii, aieea AEIA ia ?icaeoie noao?eieieiai? ?ioaeoe?? i?iyaeyany ye oi?ioaaiiy aioeaaeoa??aeueii? ?acenoaioiino?. Aca?iia ?ioiiiiaeoethth/a ae?y iie?ii??e?oieo aaeoeei oa AEAeI Aaaaoi aaeoeeiieo i?aia?ao?a canoiniaothoue oeyoii annioe?eiaaiiai aaaaeaiiy,.iai?eeeaae AEIA oa AEAeI. Na?eiciith iaoeiaith i?iaeaiith ? i?ciaiiy aca?iiae?? aaeoeei i?ae niaith. O neeaae? aaeoeeie AEAeI iiooaeiei ?ioiiiiaeoeyoi?ii ? eaoetheiaee eiiiiiaio, yeee aeineoue /anoi aeeeeea? i?eai?/aiiy ?ioiieia?/ieo ?aaeoe?e i?aai?cio oa ?icaeoie na?eicieo iaa?ieia?/ieo oneeaaeiaiue (D.Miller, 1981, M.Griffin, 1990). AEIA ni?e/eiy? a??on?iaeoeiaaio aioeaaiianiaoeeo?/io ?ioiiiiaeoeyoe?th, ci?ithth? ?ioiio a?aeiia?aeue i?aai?cio aei aaoa?iaioeaai?a o aeaeyae? ye ?ioiinoeioeyoe??, oae ? ?ioiinoi?an??. Ia?niaeoeaiei ? ii??aiyeueia aea/aiiy ?ioiiiiaeoethth/i? ae?? AEIA oa ?IA. Inoaiiy a?eueia a?ae iiaeeeaino? ?aaa?n?? iaoiaaiiino?, aea ia iiaeia aeeeth/aoe iiaeoeyoe?th ?ioiii? a?aeiia?ae? aei ?ioeo aioeaai?a ii aiaeia?? c a?aeiieie oaeoaie i?i ?ioiiiiaeoethth/o ae?th ?iaeoeaiaaieo a??onieo aaeoeei (A.O.Naiaiia, 1989). Oiio aoei aeine?aeaeaii oaiiiai ?ioiiiiaeoeyoe?? aeey AEIA oa ?IA, ?acoeueoaoe iaaaaeai? a oaae.10. Anoaiiaeaii, ui oeo?e i?ioeaeeooa??eieo aioeo?e ciaioothoueny i?ae aieeaii AEIA a 12,44 ?ace, a i?aaoeaaeo – a 16,0 ?ac. Oe? oaeoe aeaiiino?othoue iia? aoaeoe a ?ioiiiiaeoethth/?e ae?? a??on?a iie?ii??e?oo, ui caeeth/athoueny a ae?aci?e ?ioiinoi?an?? a?aeiinii ooai?aiiy aeeooa??eieo oa i?aaoeaaeo aioeoienei?a, ?iaeoeiaaieo eiiiiiaioaie AEAeI. ?aae?caoe?y oaeiai ?ioiiiiaeoethth/iai aoaeoo a?aeaoaa?oueny a oiiaao iaeii/anii? ?ioi?caoe?? aeeaith iie?ii??e?oiith aaeoeeiith oa AEAeI. ?IA ia? cia/i? ia?aaaae- cia/ii iaio? i?iyae ?ioiinoi?an??. ?IA iaea a?eueo aenieo aioeaaii?noue, oeo?e a??oniaeo?ae?coth/eo aioeo?e aei an?o o?ueio oei?a iie?ia??on?a aoee aeinoia??ii aeueie a?aeiia?aeii a 2,0; 1,43 oa 2,29 ?ace, i?ae oae? nai? i?e ?ioi?caoe?? AEIA. I?ae aieeaii AEAeI ia ?ioiio a?aeiia?aeue aei AEIA ??ai? a??oniaeo?ae?coth/eo aioeo?e aeinoia??ii ciaiooaaeenue a 3,66; 4,66 oa 3,55 ?ace aeey iie?ia??on?a ?, ??, ? ??? oei?a a?aeiia?aeii. Aca?iiae?y AEAeI c ?IA ni?e/eiyea noi?an?th aioeo?eiooai?aiiy aei iie?ia??ono ? oeio – ciaioaiiy a 1,96; ??? oeio–a 1,3 ?ace. Aenia?eiaioaeueia iaa?oiooaaiiy oaeineiiaeaiiy eaeaiaea?th uaieaiue i?ioe iie?ii??e?oo, aeeooa???, i?aaoeth ia eaai?aoi?ieo iiaeaeyo Iiia?aaei?ie aeine?aeaeaiiyie aoei anoaiiaeaii, ui AEIA ia? ae?acio ?ioiiiiaeoethth/o ae?th a?aeiinii aeeooa??eiiai oa i?aaoeaaiai aiaoienei?a a neeaae? AEAeI. Iaeii/ania ?ioi?caoe?y ieoae aaeoeeiaie AEIA oa AEAeI i?ecaiaeeoue aei aca?iii? ?ioiiiiaeoethth/i? ae??. Ia oii? ?noioiiai cieaeaiiy ??aiy aeeooa??eieo oa i?aaoeaaeo aioeoienei?a, a?aeaoaa?oueny i?eai?/aiiy neioaco a??oniaeo?ae?coth/eo aioeo?e. Iaea?eueo i?inoei oa ?aaeueiei oeyoii ei?aeoe?? aca?iii? ?ioiinoi?an?? ? ?ic’?aeiaiiy ?ioi?caoe?? a /an?. Anoaiiaeaii, ui (oaae.11) ?c ca?eueoaiiyi /ano i?ae aaaaeaiiyi aeaio aaeoeei a?ae 7 ae?a aei 21 aeiy ianoa? iioeiaeueiee aoaeo i?aaethaaiiy ?ioiinoi?aneaii? ae??. Ooai?aiiy aeeooa??eieo aioeoienei?a i?e ?ioi?caoe?? AEAeI ca 21 aeiao i?ney AEIA ca?eueoo?oueny a 16 ?ac, i?aaoeaaeo – a 21,33 ?ace; a??oniaeo?ae?coth/eo aioeo?e – a 4,02; 6,66 oa 4 ?ace a?aeiinii iie?ia??on?a ?, ??, ??? oei?a. ?icae?eaiiy a /an? ?ioi?caoe?? ?IA oa AEAeI oaeiae i?ecaiaeeei aei cia/iiai i?aeaeuaiiy aoaeoeaiino? aaeoeeiaoe??. Ia ??ai? aea/aiiy ooieoe?iiaeueii? aeoeaiino? O- oa A-e?ioioeeo?a anoaiiaeaii, ui iaea?eueo? iieacieee i?ie?oa?aoe?? iaoiiaeai? ?ioi?caoe??th AEIA oa ?IA (2035+146; 816+93). I?eai?/aiiy i?ie?oa?aoe?? e?ioioeeo?a niinoa??aaee i?e ?ioi?caoe?? ieoae aaeoeeiith AEAeI (219+24), aai i?e anioe?eiaai?e c AEAeI ?ioi?caoe?? AEIA, ?IA- a?aeiia?aeii- (444+30); (363+57). Aaciina?aaei?ie iane?aeeaie oaeeo oaiiiai?a iiaea aooe i?eai?/aiiy ?iaeoeoe?? ?ioa?oa?iio, noi?an?y aioeo?eiooai?aiiy. Ii?iae?caoe?y ooieoe?iiaeueii? aeoeaiino? e?ioioeeo?a a?aeaoaa?oueny i?e ?ic’?aeiai?e ?ioi?caoe??. Iiaeeea? iaoai?cie ?ioiiiiaeoethth/i? ae?? a??on?a iie?ii??e?oo. ?ai?iaeoeoe?y a??on?a a i?aaiao ?ioiii? nenoaie iiaea iiynithaaoe ?o ?ioiiiiaeoethth/o ae?th .Iaeiei ?c a??ia?aeieo iaoai?ci?a ?ioiiiiaeoeyoe??, ni?e/eiaii? a??onaie iie?ii??e?oo, oaeiae iiaei aooe ?o ?iciiiaeaiiy a i?aaiao ? ee?oeiao ?ioiii? nenoaie. ?ioiii?aaiio?ii?ci aaeoeeiieo a??on?a iie?ii??e?oo. Anoaiiaeaii, ui /a?ac 3 aiaeeie i?ney ?ioi?caoe?? niinoa??aa?oueny aaeeea ??cieoey e?eueeino? a??on?a a i?aaiao ?ioiii? nenoaie - oeion? ? naeac?ioe?, a ii??aiyii? c na?oeai. *a?ac 1 aeiao oaea oaiaeaioe?y i?aeoaa?aeaeo?oueny. Oeth aeanoea?noue a??on?a a aeaiiio aeiaaeeo iiaeia ioa?aeoa?ecoaaoe ye ?ioiii?aaiio?ii?ci. Aaeoeeii? oa a??oeaioi? ooaie a??on?a iie?ii??e?oo ?, I, O oeio caeaoi? aei oaeaeeiai i?iieeiaiiy ? ?ai?iaeoeoe?? a i?aaiao ?ioiii? nenoaie. Aoaioeiaai? ooaie, ia a?aei?io a?ae a??oeaioieo, iathoue a?eueo ae?aciee ?ioiii?aaiio?ii?ci, oey iciaea anioe?th?oueny c iie?ia??onaie ? ? ?? oei?a. Oaiiiai ?ioiiiiaeoeyoe??, iaoiiaeaiee ca`yceii iie?ii??e?oo c ee?oeiaie ?ioiii? nenoaie, iaiao?aeii a?aoiaoaaoe aeey oaeineiiaeaiiy i?io?eaeoeee iie?ii??e?oo, aeeooa??? oa i?aaoeth. ?ioa?oa?iiiaaiia ae?y AEIA oa AEAeI. ?OI, ye iiaeoeyoi?e ?ioiieo ?aaeoe?e, ni?e/eiythoue ieceo iiceoeaieo aoaeo?a: noeioeyoe?th oaaioeeoico, aeoeaaoe?th i?e?iaeieo e?ea??a, i?aeaeuaiiy ?acenoaioiino? ee?oei aei a??onii? ?ioaeoe??, ?acii c oei, ? ? iaaaoeai? oaiiiaie: i?eai?/aiiy aioeo?eiooai?aiiy, i?ie?oa?aoe?? e?ioioeeo?a (A.Ae. Nieiaueaa, 1981). I?e iaeii/ani?e ?ioi?caoe?? AEIA oa AEAeI iaei??th ?c i?e/ei i?eai?/aiiy aioeo?eiooai?aiiy iiaea aooe iaaei??ia i?iaeoeoe?y oeueiai iaae?aoi?o. Iieacaii, ui AEIA, ye ? ?? neeaaeia? eiiiiiaioe- aoaioeiaai? ooaie Naa?io a??on?a iie?ii??e?oo (E.A.O?ieiaa, 1993), ?iaeoeo? ae?acio i?iaeoeoe?th ?OI. Iaeneioi neioaco ne?iaaoeiaiai ?OI AEIA ?iaeoeo? ia 1 aeiao aeine?aeaeaiiy – 586,6( 51,53 II. Aaeoeeia AEAeI aoea cia/ii neaae?oei ?iaeoeoi?ii ?OI, i?e neioaco a?aeaoaaany ia 4 aeiao –133,3 ( 25,76 II I?e noi?ni?e ?ioi?caoe?? iaiia aaeoeeiaie niinoa??aaee iaea?eueoo i?iaeoeoe?th ?OI, yea ia 1 aeiao– 1066,7(103,1– a 1,81 ?ace ia?aaeuoaaea oaeee iieaciee aeey AEIA, aeae? e?euee?noue ?OI no??iei ciaiooaaeany Oae? aoaeoe i/aaeaeii iia”ycai? c aaeeeei aioeaaiiei iaaaioaaeaiiyi - “oieii” aeey ?ioiii? nenoaie iae?aco i?ney ?ioi?caoe??. Aeniea i?iaeoeoe?y ?OI iiaea aooe oe?aeeeaith aeey ?aae?caoe?? ?aaeoe?e aoii?aeueiiai ?ioi?oaoo. I?e ?ic”?aeiaii? ?ioi?caoe?? a /an? niinoa??aaee oaiaeaioe?th aei ii?iae?caoe?? ??aiy ?OI ia ia?oo aeiao –213,3(25,76. Oeueo?ano?oeoo?ia i?aai?caoe?y i?aai?a ?ioiiiai caoenoo i?e ??cieo oeiao aaeoeeiaoe?? aoea aea/aia o ieoae aeey i?ciaiiy iaoai?ci?a ?ioiiiiaeoethth/i? ae?? aaeoeei. A oeion? oa naeac?ioe? i?ae aieeaii AEIA aeyaeyee ?aaeoe?th e?ioi?aeii? nenoaie ??cii? ?ioaineaiino?. Aeoeaaoe?y nieaiioeeo?a, oeiioeeo?a, ieaciioeeo?a oa ee?oei Oaaeeoey 10 Aca?iia ?ioiiiiaeoethth/a ae?y aaeoeei AEIA oa AEAeI. A?oie ieoae. Aa??aioe ?ioi?caoe?? Aioeoieneie Aioeo?ea, ui iaeo?ae?cothoue iie?ia??one Aeeooa??ei? I?aaoeaa? ? oeio ?? oeio ??? oeio Eiio?ieueia a?oia. (n=30) ?ioi?caoe?y AEIA. 0 0 57,6+6,15 89,6+10,3 51,2+6,15 Aeine?aeia a?oia – iaeii/ania ?ioi?caoe?y AEIA oa AEAeI. (n=30) 18+1,92 p<0,001 72+5,15 p<0,001 16(1,3 p<0,001 19,2+3,2 p<0,001 14,4+1,6 p<0,001 Eiio?ieueia a?oia. ?ioi?caoe?y ?IA. (n=30) 0 0 115,2+10,3 128(4,1 117,3+10,3 Aeine?aeia a?oia – iaeii/ania ?ioi?caoe?y ?IA oa AEAeI. (n=30) 192+17,87 ?(0,1 768+12,3 p<0,001 58,6+5,16 p<0,01 128(5,2 ?(0,1 89,6+8,93 p<0,05 Eiio?ieueia a?oia. ?ioi?caoe?y AEAeI. (n=30) 224+18,28 1152+12,3 0 0 0 Oaaeeoey 11 Aenia?eiaioaeueia iaa?oiooaaiiy aoaeoeaieo aa??aio?a ?ioi?caoe?? aeey i?io?eaeoeee iie?ii??e?oo, aeeooa???, i?aaoeth. A?oie ieoae, aa??aioe ?ioi?caoe?? Aioeoieneie Aioeo?ea, ui iaeo?ae?cothoue iie?ia??one Aeeooa??ei? I?aaoeaa? ? oeio ?? oeio ??? oeio ?ioi?caoe?y iaeii/anii AEIA oa AEAeI (n=30) 18+1,92 72+5,15 16(1,3 19,2+3,2 14,4+1,6 ?ioi?caoe?y AEIA, /a?ac 7 aei?a AEAeI (n=30) 21+3,12; ?(0,1 96+15,38; ?(0,1 38,4+2,15; p<0,01 32(1,4; p<0,02 28,8+3,2 p<0,001 ?ioi?caoe?y AEIA, /a?ac 14 aei?a AEAeI (n=30) 48+7,69; p<0,01 818+20,4;p<0,01 54(3,12; p<0,001 78,7(3,1; p<0,001 45,3(2,6 p<0,001 ?ioi?caoe?y AEIA, /a?ac 21 aeaiue AEAeI (n=30) 288+30,7;p<0,001 1536+123;p<0,001 64,4(2,4; p<0,001 128(6,2 p<0,001 57,6+6,15 p<0,001 ?ioi?caoe?y iaeii/anii ?IA oa AEAeI (n=30) 192+17,87 768+12,3 58,6(5,16 128(5,2 89,6(8,93 ?ioi?caoe?y ?IA, /a?ac 21 aeaiue AEAeI (n=30) 448+30,7; p<0,001 2792+246;p<0,001 101,3(7,6; p<0,001 109,2(4,8; p<0,05 99,4(10,9; ?(0,1 I?ei?oee: 1. Oeeo?aie a oaaeeoeyo i?aaenoaaeai? oeo?e aioeoiene/ieo oa a??oniaeo?ae?coth/eo aioeo?e a na?aaei?o cai?ioi?o aaee/eiao ?icaaaeaiue ne?iaaoie. 2.?-??cieoey iieaciee?a i?ae aeine?aeieie oa eiio?ieueieie a?oiaie iae?ioaaaeueii? nenoaie, ?aaeoe?y i?e?ioee?eoeyoi?iiai ?onea na?ae/eee i?i yaia ?iaeoeoaaiiy ?ioiii? a?aeiia?ae?.?IA oaeiae ni?eyea aeoeaaoe?? ?ioiiaaiaco. AEAeI i?ecaiaeeea aei iioeiaeaeoth/eo ci?i no?oeoo?ii? i?aai?caoe?? naeac?iee: a?aei?/aee ii?ooaiiy ?aieia?? e?ia?, iaoo?aoe?th noaeei oi?iaiieie aeaiaioaie, ia?eaaneoey?ia neoi/aiiy eieaaaiiaeo aieieii, o?aaiaioe oeeoiieacie ooeiaeaeaiieo ee?oei no?iie, eaeeioeeo?a oa iae?ioaa?a. *anoeia ?ioiieiiiaoaioieo ee?oei ciaoiaeeeanue a noai? ooieoe?iiaeueii? aeoeaiino?, ui oeueo?ano?oeoo?ii i?iyaeyeinue ca?eueoaiiyi a ieo e?eueeino? ?eainii, iie?nii, i?oioiiae??e oa ae?ino?a ieaciaoe/ii? iaia?aie. I?e iaeiiiiiaioi?e ?ioi?caoe?? AEIA ? AEAeI ae?aaeai? ci?ie a?aecia/aee a aaiii?e?ioee?eoeyoi?iiio ?one?, aea i?ina?oe noaeei, ye i?aaeei, aoee iaoo?iaai? o?iiaioeeoaie, eaeeioeeoaie oa a?eo?ioeeoaie. Aiaeia?/i? ''neaaeae oaiiiaie'' aeyaeyee a e?iaiiinieo eai?ey?ao, a aaioey?i?e eaioe? aaiii?e?ioee?eoeyoi?iiai ?onea. ?ioiieiiiaoaioi? ee?oeie caciaaaee aeano?oeoeaieo oa aeeno?io?/ieo ci?i. I?e ?ioi?caoe?? ieoae ?IA iaeii/anii c AEAeI a naeac?ioe? a?aecia/aee ci?ie a aaiii?e?ioee?eoeyoi?iiio ?one?, iia’ycai? c iioeiaeaeaiiyi aiaeioae?th oeeo noaeei, ?icoiaeaeaiiyi aiaeioae?aeueieo ee?oei oa aeyaeaiiyi oi?iaiieo aeaiaio?a e?ia? ia?eaaneoey?ii. No?oeoo?ia i?aai?caoe?y iae?ioaa?a ? ieaciaoe/ieo ee?oei aeecueea aei eiio?ieueieo. Aaaaeaiiy aaeoeeie AEAeI /a?ac 21 aeaiue i?ney ?ioi?caoe?? AEIA iieacaei, ui a naeac?ioe? aeaui iineethaaeenue ?aaeoeai? ci?ie c aieo aaiii?e?ioee?eoeyoi?iiai ?onea oa no?iie. Aeoeaoaaeeny iae?ioaae oa ieaciaoe/i? ee?oeie, ca?eueooaaeanue ?o e?euee?noue oa a?aecia/aeanue a?ia?ieac?y ee?oeiieo i?aaiae. A a?e?e ioeuei? naeac?iee ia oii? aeoeaaoe?? e?ioioeeo?a a?aei?/aee a?aenooi?noue ae?acieo ii?ooaiue ?o oeueo?ano?oeoo?ii? i?aai?caoe??, ui, i/aaeaeii, iia’ycaii c ?icaeoeii eiiiainaoi?ii-i?enoinoaaeueieo i?ioean?a a i?aai?.Oaeei /eiii, i?iaaaeaia aeaeo?iiii-i?e?ineii?/ia aeine?aeaeaiiy naeac?iee ? /anoeiai oeiono i?e ae?? aaeoeeiieo i?aia?ao?a iieacaei, ui aiie a iaai?e i??? iaee ooeiaeaeoth/o ae?th ia ?ioiieiiiaoaioi? ee?oeie naeac?iee oa oeiono. I?iyae oeueiai aoaeoo aoee iaiaeiaeiaeie. Iaea?eueo ae?aaeaiee iaaaoeaiee aoaeo i?eoaiaiiee ia’?aeiai?e ?ioi?caoe?? (AEIA+AEAeI), a iaeiaio?e ?IA. I?e ?icae?eueiiio (/a?ac 21 aeiao) aaaaeaii? AEIA oa AEAeI oi?ioaaeenue eiiiainaoi?ii-i?enoinoaaeuei? i?ioeane ? ciaiooaaeanue e?euee?noue ooeiaeaeaieo ?ioiieiiiaoaioieo ee?oei. Oaea aaaaeaiiy aaeoeei i?ecaiaeeei aei iaee?auiai ?ioiinoeioethth/iai aoaeoo. Ei?aeoe?y aca?iii? ?ioiiiiaeoethth/i? ae?? aaeoeei AEIA oa AEAeI a i?ioean? ?ioi?caoe?? ae?oae i?ioe iie?ii??e?oo, aeeooa???, i?aaoeth aoea i?iaaaeaia ia aac? aeeoy/i? iie?ee?i?ee i.I?eeoee *a?i?a?anueei? iaeano? ca aeicaieii canooiieea Aieiaiiai aea?aeaaiiai nai?oa?iiai e?ea?y Oe?a?ie A.A.Iaae/aiei, eeno ?5.09.19/711 a?ae 11.09.1997 ?ieo Aieiaiiio aea?aeaaiiio nai?oa?iiio e?ea?th *a?i?a?anueei? iaeano? Aeiioeth I.I. Ca aeiiiiiao a i?aai?caoe?? oa i?iaaaeaii? ?iaioe c ae?oueie aeneiaeth?ii ue?o iiaeyeo niaoe?ae?noai ioi?iie caei?ia”y i.I?eeoe. Aoee noai?ai? aea? a?oie ae?oae, a ia?o?e a?oi? aoei ianoaaeaii 18 ae?oae, ia?iaeaeaieo a a?oaei? 1997 ?ieo. Aiie aoee aaeoeeiiaai? iaeii/anii AEIA oa AEAeI aea?/?, c ?ioa?aaeii iaeei i?nyoeue a aa?aci? oa ea?oi? 1998 ?ieo. ae?oaa a?oia – 16 ae?oae, ia?iaeaeaieo a aa?aci? 1998 ?ieo, ?ioi?caoe?th a oe?e a?oi? i?iaaee aaeoeeiaie AEIA oa AEAeI iine?aeiaii c ?ioa?aaeii iaeei i?nyoeue, a /ioe?e aoaie. Ae?oaa a?oia ae?oae iaea?aeaea aiaeia?/io ?c ia?oith a?oiith e?euee?noue aaeoeeiaeueieo aeic aoaioeiaaieo a??on?a iie?ii??e?oo oa aiaoienei?a, aea a ??ci?e iine?aeiaiino?.(oaae.12). Oaaeeoey 12 Aoaeoeai?noue ?icae?eueii? oa noi?nii? ?ioi?caoe?? ae?oae aaeoeeiaie AEIA oa AEAeI. Aeaee aioeo?e Oeo?e aioeo?e (O AO) Iaeii/ania ?ioi?caoe?y ?icae?eueia ?ioi?caoe?y Aaee/eie O AO E?euee?noue ae?oae a % Aaee/eie O AO E?euee?noue ae?oae a % Aeeooa??ei? aioeoieneie Na?aaei?e 38,8 ( 8,8 72,5 ( 8,5; ? (0,02 Iaeneiaeueiee 160 5,55 160 18,75 I?i?iaeueiee 20 50,0 40 37,5 ( i?ioaeoeaiiai ( 40 55,55 ( 40 12,5 I?aaoeaa? aioeoieneie Na?aaei?e 82,2 ( 17,7 125 ( 17,0; ? (0,1 Iaeneiaeueiee 320 5,55 320 6,25 I?i?iaeueiee 40 50,0 80 56,27 ( i?ioaeoeaiiai ( 20 0 ( 20 0 Iaeo?ae?coth/? a??on iie?ii??e?oo ? oeio Na?aaei?e 85,8 ( 31,3 312 ( 27,25; ? (0,001 Iaeneiaeueiee 256 5,55 512 31,25 I?i?iaeueiee 16 5,55 128 18,75 Iaeo?ae?coth/? a??on iie?ii??e?oo ?? oeio Na?aaei?e 174, 22 ( 26,6 296 ( 38,0; ? (0,02 Iaeneiaeueiee 512 11,11 1024 6,25 I?i?iaeueiee 32 22,22 64 12,5 Iaeo?ae?coth/? a??on iie?ii??e?oo ??? oeio Na?aaei?e 132,44 ( 31,3 200 ( 34,0;?(0,1 Iaeneiaeueiee 512 5,55 512 12,5 I?i?iaeueiee 16 5,55 32 31,25 I?ei?oea: ? -??cieoey iieaciee?a na?aaei?o oeo??a a aaee/eiao, cai?ioi?o ?icaaaeaiiyi ?acoeueoaoe ii??aiyeueiiai aiae?co iaio aa??aio?a ?ioi?caoe?? – iaeiiiiiaioii? oa ?icae?eueii? a /an?, iaaaaeai? a oaae.12. ?icae?eueia ?ioi?caoe?y ia? aacia?a/i? ia?aaaae a ii??aiyii? c iaeii/aniith: na?aaei?e oeo? aeeooa??eieo aioeoienei?a a 1,86 ?ace aeua, iaeneiaeueiee oeo? aioeo?e – 160 aeyaeyee a 3,37 ?ace /ano?oa, i?i?iaeueiee oeo? aeey iaeii/anii? ?ioi?caoe?? – 20, aeey ?icae?eueii? – 40, ? ia?aoo? e?euee?noue ae?oae, ui ia iaee i?ioaeoeaiiai oeo?o (40) aoei iaioei ia 43,05% i?e ?icae?euei?e ?ioi?caoe??. Aioeoiene/iee i?ioe-i?aaoeaaee ?ioi?oao a?ae??ciyany ca aaee/eiith na?aaeiueiai oeo?o aioeoienei?a, aiia aoea aeaui a?eueoith – 125,0+17,0 i?e ?icae?euei?e ?ioi?caoe??, i?ae 82,2+17,7 i?e noi?ni?e, aea ?io? iieacieee ?noioii ia a?ae??ciyeeny. Na?aaei? oeo?e a??oniaeo?ae?coth/eo aioeo?e cia/ii a?ae??ciyeenue i?e ?icae?euei?e ?ioi?caoe??, aei a??ono iie?ii??e?oo ? oeio na?aaei?e oeo? ca?eueoeany a 3,63 ?ace, aei ?? oeio – a 1,69 ?ace, aei ??? oeio – a 1,51 ?ace. Ii??aiyiiy aeaio noai ?ioi?caoe?? aoei i?iaaaeaii eeoa a i?ioean? aaeoeeiaoe??. Aea aaea ca?ac iiaeia aa/eoe, ui oi?ioaaiiy ?ioi?oaoo na?ae/eoue i?i cia/io ei?enoue ?ic’?aeiaii? a /an? ?ioi?caoe?? a ii??aiyii? ?c iaeii/aniith aaeoeeiaoe??th. AENIIAEE Iieacaii, ui i?ae /an ?ai?iaeoeoe?? a iiioeyoe?yo aaeoeeiieo oa a??oeaioieo a??on?a iie?ii??e?oo a?aeaoaa?oueny aeenioe?aoe?y ia aaiaoe/i? aa??aioe c aenieei (Aaaio+) oa iecueeei ao?i?oaoii aei aaioii?oo (Aaaio(). A e?euee?niiio neeaae? iiioeyoe?e aaeoeeiieo iie?ia??on?a aeii?iothoue aa??aioe Aaaio+, ai?no aa??aio?a Aaaio( ? a?ae 0,02% aei 5,9% oa 0,36% - 0,39% - aeey a??oeaioieo a??on?a iie?ii??e?oo ??-??? oei?a. A??oeaioiee iie?ia??on ? oeio Mahoney ?nio? a oi?i? Aaaio( -97,91% aa??aioa a caaaeuei?e iiioeyoe??. Aa??aioe Aaaio+ aaeoeeiieo oa a??oeaioieo iie?ia??on?a iathoue cia/ii aeuo a?ieia?/io aeoeai?noue, ?o aioeaaii?noue i?e ii??aiyii? c aa??aioaie Aaaio( a na?aaeiueiio a?eueoa a 2,96 ?ace o aaeoeeiieo ooai?a oa a 1,93 ?ace o a??oeaioieo. Aeiyoeii ? a??oeaioiee iie?ia??on ? oeio Mahoney, eiai aioeaaii?noue a?eueo ae?aaeaia o aa??aioa Aaaio(. ?ioiiaaii?noue aa??aio?a Aaaio+ aaeoeeiieo iie?ia??on?a ?? oeio oaeiae aeua i?ae o Aaaio(, ??cieoey i?ioaeoeaiiai aoaeoo ?ioi?caoe?? i?e aenia?eiaioaeuei?e iie?ii??e?oi?e ?ioaeoe?? aeinyaa? 63,7%. I?ia?aeia ?ieue a iaoiaaiac? aenia?eiaioaeueii? iie?ii??e?oii? ?ioaeoe?? anioe?eiaaia c aa??aioii Aaaio+. Aeiaaaeaii, ui aaiaoe/iee aa??aio Aaaio+ a iiioeyoe?yo a??oeaioieo a??on?a iie?ii??e?oo ?? oeio MEF-1 a?ae??ciy?oueny a 2,36 ?ace aeuith iae?ia??oeaioi?noth aeey ieoae, i?ae Aaaio( (ii??aiyiiy ca aaee/eiith na?aaeiuei? o?eaaeino? aeeooy). Aa??aioe Aaaio+ oa Aaaio( ?ioaineaii i?iieeathoue a oeion oa naeac?ieo a ia?o? aiaeeie ?ioaeoe??, aiie ooeiaeaeothoue no?oeoo?o ?ioiieiiiaoaioieo ee?oei, aa??aio Aaaio( iaoiiaeth? aiiioic e?ioioeeo?a naeac?iee. Ia aenia?eiaioaeueieo iiaeaeyo aeyaeaii, ui Aaaio+ oa Aaaio( aa??aioe iiioeyoe?e a??on?a iie?ii??e?oo ni?aaeythoue ??ciiiai?oiee aieea ia ?ioiio nenoaio. I?ia?aeia ?ieue a ?iaeoeoe?? ooai?aiiy a??oniaeo?ae?coth/eo aioeo?e oa a aeanoiaaiac? e?ioioeeo?a iaeaaeeoue aa??aioo Aaaio+ aeey a??oeaioieo oa aaeoeeiieo ooai?a. Aia?oa aeey a??on?a iie?ii??e?oo ia ??ai? iiioeyoe?e iieacaii, ui aiie iathoue ae?acio ?ioiiiiaeoethth/o ae?th. ?? i?iyaii ? i?eai?/aiiy aoii?aeueii? oa ee?oeiii? ?ioiii? a?aeiia?ae? aei aaoa?ieia?/ieo aioeaai?a. ?ioiinoi?an?y a?eueoith i??ith iia”ycaia c aa??aioii iiioeyoe?? Aaaio( : i?eai?/aiiy aioeo?eiooai?aiiy aei aaoa?ia?eo?ioeeo?a ia 71,55%, aeey Aaaio+ -ia 64,23 %; ciaioaiiy i?iya?a I*NO aei aaoa?ioeiioeeo?a aa??aioii Aaaio( ia 83,1%, Aaaio+ ia 42,8% (ia i?eeeaae? aaeoeeiiiai iie?ia??ono ??? oeio). Aoaioaoe?y iie?ia??on?a ia aieeaa? ia i?iya ?o ?ioiinoi?aneaii? ae??. ?ioiiiiaeoethth/a ae?y iiioeyoe?e a??on?a iie?ii??e?oo aeine?aeaeaia i?e aca?iiae?? aaeoeei- AEIA oa AEAeI. Iaeaea? aaeoeeie i?e iaeii/aniiio aaaaeaii? aca?iii i?eai?/othoue aioeoiene/iee ?ioi?oao (aeeooa??eiee a 12,44 ?ace, i?aaoeaaee a 16 ?ac) oa i?ioea??oniee ?ioi?oao (ciaioaiiy oeo??a aioeo?e aei iie?ia??on?a ?, ??, ??? oeio a?aeiia?aeii a 3,66; 4,66 oa 3,55 ?ac). ?iaeoeaiaaia iie?ii??e?oia aaeoeeia (?IA) ia? a?eueo aenieo i?ae AEIA aioeaaiio ae?th, oeo?e aioeo?e aei an?o o?ueio oei?a iie?ia??on?a aoee aeueie a 2; 1,43; oa 2,29 ?ac a?aeiia?aeii. I?e iaeii/ani?e ?ioi?caoe?? ?iaeoeiaaia ?IA ?ioiinoi?an?y aoea cia/ii iaioith, i?ae aeey AEIA. I?ae aieeaii AEAeI ooai?aiiy a??oniaeo?ae?coth/eo aioeo?e ?iaeoeiaaieo ?IA caciaaaei iaioi? noi?an??, ui na?ae/eoue i?i ?? ?noioi? ia?aaaae. Aia?oa aeey a??on?a iie?ii??e?oo iieacaii, ui i?e/eiith ?o ?ioiiiiaeoethth/i? ae?? ? ?ioaineaia oa oaeaeea (ca ia?o? 3 aiaeeie) i?iieeiaiiy a naeac?ieo oa oeion, oaiiiai iacaaiee iaie ?ioiii?aaiio?ii?ci. A?i a?eueo ae?aaeaiee aeey aaeoeeiieo ooai?a ?, ?? oei?a, i?ae aeey a??oeaioieo. Aeiaaaeaii, ui ?ioeie iaoai?ciaie, ui ni?eythoue aca?ii?e ?ioiinoi?an?? AEIA oa AEAeI iiaeooue aooe ii?ooaiiy ?iaeoeoe?? ?ioa?oa?iio oa ooeiaeaeaiiy oeueo?ano?oeoo?e ee?oei a i?aaiao ?ioiiiai caoenoo i?e iaeii/ani?e ?ioi?caoe??, i?e ?ic”?aeiaii? aaeoeei a /an? (21 aeiaa) iaaaoeai? aoaeoe iinooiiai i?aaeththoueny. Aenia?eiaioaeueii iaa?oioiaaii oa cai?iiiiiaaii aeey ei?aeoe?? aca?iii? ?ioiiiiaeoethth/i? ae?? AEIA oa AEAeI ?o ?ic”?aeiaia aaaaeaiiy. Ia iiaeaeyo e?i?eieo ieoae anoaiiaeaii, ui ?ioa?aae 21 aeaiue i?ae ?ioi?caoe??th AEIA (nii/aoeo) oa AEAeI i?ecaiaeeoue aei iaeo?ae?caoe?? ?ioiinoi?an?? (oeo?e aeeooa??eieo oa i?aaoeaaeo aioeoienei?a a?aeiia?aeii i?aeaeuoaaeenue a 16, oa 21,33 ?ac; aioeo?e iaeo?ae?coth/eo iie?ia??one oei?a ?, ??, ??? a 4,02; 6,66; 4 ?ac a?aeiia?aeii. Aoaeoeai?noue ?icae?eueii? ?ioi?caoe?? aeiaaaeaia i?e aaeoeeiaoe?? AEIA oa AEAeI aeaio a?oi ae?oae i. I?eeoee ca i?aeaeuaiiyi na?aaei?o oeo??a a??oniaeo?ae?coth/eo aioeo?e a 3,63; 1,69; 1,51 ?ac a?aeiia?aeii aei oei?a iie?ia??on?a ?, ??, ???; ciaioaiiyi e?eueeino? ae?oae aac i?ioaeoeaiiai oeo?o aeey aeeooa??? ia 43,05% oa ca?eueoaiiyi /enea ae?oae c iaeneiaeueiei oeo?ii aeeooa??eieo aioeoienei?a- 160 ia 13,2%. I?AEOE*I? ?AEIIAIAeAOe?? Aeenioe?aoe?y iiioeyoe?e aioa?ia??on?a (iie?ii??e?oo, Eienae?), yea iino?eii a?aeaoaa?oueny i?ae /an ?o ?ai?iaeoeoe?? a i?e?iae? oa eaai?aoi?ieo oiiaao i?ecaiaeeoue aei aaoa?iaaiiino? a ?o neeaae? oa iiyae aa??aio?a c ??cieie o?ceei-o?i?/ieie oa a?ieia?/ieie oa?aeoa?enoeeaie. Ia oeae oaiiiai iaiao?aeii caa?oaoe iino?eio na?eicio oaaao i?e ae?aaiinoeoe?, i?io?eaeoeoe? a?aeiia?aeieo ?ioaeoe?e. Iniaeeaiai cia/aiiy aaoa?iaaii?noue iiioeyoe?e aaeoeeiieo ooai?a c ??cieie aioeaaiieie, ?ioiiaaiieie oa ?ioiiiiaeoethth/eie aeanoeainoyie ia? aeey ae?iaieoeoaa aeeaeo iie?ii??e?oieo aaeoeei. Aeey ciaioaiiy aca?iii? ?ioiiiiaeoethth/i? ae?? aaeoeei AEIA oa AEAeI i?e ?ioi?caoe?? ae?oae aeioe?eueii aaiaeeoe ?o ie?aii, c ?ioa?aaeii 1 i?nyoeue, ?icii/eiaoe noaio uaieaiue aaeoeeiaoe??th AEIA. Aaeoeeia ?IA, yea iaeaea ia ia? ?ioiinoi?aneaii? ae??, iiaea aooe canoiniaaia aeey ?ioi?caoe?? ae?oae c ?ioiiaeao?oeeoaie oa i?e niaoe?aeueieo iieacaiiyo. NIENIE ?IA?O IIOAE?EIAAIEO CA OAIITH AeENA?OAOe?? Oe?iaieia A.I., Atheeeia Y.A., Ei?ithoaiei I.I., Iaa?aaaoeeay Y.I., ?icaioaeueae I.E. Yenia?eiaioaeueiia iainiiaaiea i?eiaiaiey eiioiiiiaeoeyoi?ia oeia oaieeeieaeacioeacieia aeey ei??aeoeee aoi?e/iie eiioiieiae/aneie iaaeinoaoi/iinoe, iaoneiaeaiiie ae?onaie iieeiieaeeoa.// A?a/aaiia aeaei.- 1985.- N 6.- N. 95-97. Oe?iaieia A.I., AtheeeiaY. A., Ei?ithoaiei I.I., Iaa?aaaoeeay Y.I. Eiaeooee?iaaiiay iieeiae?onaie noi?anney o iuoae e aiciiaeiinoe aa ei??aeoeee.// Iee?ia. aeo?i.- 1986.- o. 48.- N1.- N. 86-90. Oe?iaieia A.I., Ei?ithoaiei I.I., Iaa?aaaoeeay Y.I. Aeeyiea aaioiieoiauo aa?eaioia ae?onia iieeiieaeeoa ia oaeoi?u eeaoi/iiai e aoii?aeueiiai eiioieoaoa o iuoae.// Iee?ia. aeo?i.- 1986.- o. 48.- N 2.- N. 63-67. Oe?iaieia A.I.., Ii?aoiia E.I., Ei?ithoaiei I.I., Aa?eaaay A.A, Iaa?aaaoeeay Y.I.,Oeaai I.I.. Aeeyiea eeioieaeiiai eaeeiia ia neioac aioeoae e ae?onai iieeiieaeeoa.// Eiioiieiaey e aeea?aey. ?ani. iaaeaaae. nai?iee.-E.-1986.-a.20.-N.80-83. Oe?iaieia A. I., Ei?ithoaiei I. I., Iaa?aaaoeeay Y. I., Oeaai I. I. Eco/aiea ia?ea?ia ae?oeaioiinoe aooaioe?iaaiiuo ae?onia iieeiieaeeoa e eo naeaeoeeiie?iaaiiuo aa?eaioia.// Iee?ia. aeo?i.- 1986.- o. 48.- N 1.- N. 90-95. Oe?iaieia A.I., Atheeeia Y.A., Ei?ithoaiei I.I., Iaa?aaaoeeay Y.I. Eiioiinoi?anneaiia aeaenoaea aeeaie iieeiae?oniie aaeoeeiu e aiciiaeiinoe iaaeeeaiaioiciie ei??aeoeee.// Aeooaeueiua aii?inu eiioiieiaee e eiioiiiaoieiaee. Na iao/i. o?oaeia ?inoianeiai oieaa?neoaoa-1988.-N.52-54. Oe?iaieia A. I., Aaoooaiei A E., Eiaaeaaa I.I., Ei?ithoaiei I. I., Aieei I.E. 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Oaeineiiaeaiiy eaeaiaea?y aaeoeeiaoe?e uiaei uaieaiue AEAeI oa AEIA ca aeaiieie aeaeo?iiii? i?e?ineii??.// A?niee A?eioea?e?anueeiai Aea?aeaaiiai Aa?a?iiai Oi?aa?neoaoo.- 1998.- a.6, /.2, Ii?oieia?y – i?aeoe/i?e aaoa?eia??? oa iaaeeoeei?.-N.28-30. Ei?ithoaiei I. I. Ooeiaeaeoth/a ae?y noi?nii? ?ioi?caoe?? aeeaith iie?ii??e?oiith aaeoeeiith oa AEAeI ia ?ioiieiiiaoaioi? ee?oeie.// A?niee A?eioea?e?anueeiai Aea?aeaaiiai Aa?a?iiai Oi?aa?neoaoo.-1998.- a.6, /.2, Ii?oieia?y – i?aeoe/i?e aaoa?eia??? oa iaaeeoeei?.-N. 149-152. Ei?ithoaiei I. I. A?ieia?/i? aeanoeaino? a??on?a iie?ii??e?oo ia iiioeyoe?eiiio ??ai?.// A?niee i?iaeai a?ieia?? oa iaaeeoeeie.-1998.- ?24.-N.22-24. Ei?ithoaiei I. I. ?ioa?oa?iiiaaiia aeoeai?noue AEIA oa AEAeI. // A?niee i?iaeai a?ieia?? oa iaaeeoeeie.-1998.- ?23.-N.62-65 Ei?ithoaiei I. I. A??one iie?ii??e?oo. Oeyoe a iiaa i?ciaiiy.// I?iaeaie iaaeeoeeie.-1998.- ?4.-N.12-15. Ei?ithoaiei I. I. Iiaeeeaino? ei?aeoe?? noaie aaeoeeiaoe?? ae?oae i?ioe iie?ii??e?oo, aeeooa???, i?aaoeth. // A?niee i?iaeai a?ieia?? oa iaaeeoeeie.-1999.- ?2.-N.85-88. Ei?ithoaiei I. I. Ci?ie a i?aaiao ?ioiiiai caoenoo i?e ??cieo noaiao aaeoeeiaoe?? AEIA, ?IA, AEAeI. // A?niee i?iaeai a?ieia?? oa iaaeeoeeie.-1999.- ?2.-N.105-108. Ei?ithoaiei I. I. ?ioiiiiaeoethth/? aoaeoe aioa?ia??on?a. // A?niee i?iaeai a?ieia?? oa iaaeeoeeie.-1998.-?23.-N.50-53. Ei?ithoaiei I. I., Oe?iaieia A.I., Ao?ai??aa I.A, Aiae???iei O.?., Eicae A.?. Oeueo?ano?oeoo?i? ci?ie i?aai?a ?ioiii? nenoaie i?ae aieeaii a??on?a iie?ii??e?oo.// A?niee A?eioea?e?anueeiai Aea?aeaaiiai Aa?a?iiai Oi?aa?neoaoo.- 1998.- a.6, /.2, Ii?oieia?y – i?aeoe/i?e aaoa?eia??? oa iaaeeoeei?.-N.30-33. Oe?iaieia A. I., Ei?ithoaiei I. I., Aiiniaa E. I. oa ?i. ?ieue aioa?ia??on?a a ia?ioaeoe?ei?e iaoieia?? // I?iaeaie iaaeeoeeie.-1998.-?3.-N. 20-25. Aonaaa N. A., Aaiaeaea E.I.,. Oe?iaieia A.I, Ei?ithoaiei I. I., Aieeiaa O.A.- Iaoaio RU ?2068997 N1 ia ecia?aoaiea. Niinia ii?aaeaeaiey ianiaoeeoe/aneie aioeaaeoa?eaeueiie ?acenoaioiinoe i?aaiecia.-10.11.1996. I?eiaiaiea aaioiieoa aeey auyaeaiey yioa?iae?onia o /aeiaaea e ai aiaoiae n?aaea. (Iaoiaee/aneea ?aeiiaiaeaoeee). Oe?iaieia A. I., Ae?ei A. I, sseeiaiei A.E., Caieyineee A.A., Ei?ithoaiei I. I., Aaoooaiei A.E., Naeaoa I.A., Oaoaiaaa A.A., Aieei E.E., AEo?aa O.A. Eeaa.- 1986.- 21 n. Oe?iaieia A. I., Ei?ithoaiei I.I., Iaa?aaaoeeay A. I. Eiioiiaeai?anneaiua naienoaa aooaioe?iaaiiuo ae?onia iieeiieaeeoa // Oacenu 5-ai neiiiceoia ii aeea?aieiaee e eeeie/aneie eiioiieiaee nioeeaeenoe/aneeo no?ai.-Aieaa?ey, Aa?ia.- 1985.- N. 181-182 Ei?ithoaiei I.I.,Atheeeia Y. A., Oa?ania O. I., Iaa?aaaoeeay Y. I Yenia?eiaioaeueiia iainiiaaiea eiioiioa?aiee yioa?iae?oniuo eioaeoeee //Iaoa?. Anan. eiioa?. "Yioa?iae?onu. Iauaoai?aoe/aneea e iaaeeoeeineea aniaeou”.- Eeaa.-1991.- N. 64-65 Oe?iaieia A. I., Ei?ithoaiei I.I., Nu/aaa A. A., Oeeia I. A. Aeieiae/aneea naienoaa aaeoeeiiuo ooaiiia ae?onia iieeiieaeeoa //Iaoa?. Anan. eiioa?. "Yioa?iae?onu. Iauaoai?aoe/aneea e iaaeeoeeineea aniaeou”.- Eeaa.-1991.- N.103-104.. Oe?iaieia A. I., Ei?ithoaiei I.I., Ne/iaa A. A., Oeeia I. A. AEeaa iie?ia??onia aaeoeeia: i?iaeaie iiaeaeueoiai canoinoaaiiy // Oace IV eiia?ano na?o. oaae. oe?. e?ea?. oiaa?enoa.- Oa?e?a.- 1992.- N. 130. Shirobokov V., Kornushenko O., Melnick V., Jurba T., Shilov M. Enteroviruses dissociation phenomenon: physicochemical and biological properties of dissociants//Abstracts10th International virology.- congress –Jerusalem.- 1996.- P.95. Shirobokov V., Kornushenko O., Sitchova V., Shilov M., Achramejeva N. Polioviryses as immunomodulators //Abstracts 10th International virology congress.- Jerusalem.- 1996.- P.238. AIIOAOe?? ei?ithoaiei I.I. ?ioiiiaoiaaiac aenia?eiaioaeueiiai iie?ii??e?oo c iiceoe?e aaoa?iaaiiino? iiioeyoe?e iie?ia??on?a.- ?oeiien. Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy aeieoi?a a?ieia?/ieo iaoe ca niaoe?aeuei?noth 03.00.06 a??onieia?y.- Iaoe?iiaeueiee iaaee/iee oi?aa?neoao ?i. I.I.Aiaiiieueoey, Ee?a, 1999. Aeena?oaoe?y i?enay/aia aeine?aeaeaiith ?ioiia?ieia?/ieo aeanoeainoae aa??aio?a aaoa?iaaiieo iiioeyoe?e aaeoeeiieo oa a??oeaioieo a??on?a iie?ii??e?oo. Iieacaii, ui a i?ioean? ?ai?iaeoeoe?? iie?ia??one aeenioe?ththoue ia aa??aioe (Aaaio+ oa Aaaio() c ??ciei ao?i?oaoii aei i?e?iaeiiai ni?aaioo aaioii?oo oa aaiioeiiaeie oa?aeoa?enoeeaie: a??oeaioi?noth, aioeaaii?noth, ?ioiiaaii?noth, ?ioiii?aaiio?ii?ciii, ?ioiiiiaeoethth/eie aeanoeainoyie. Aeenioe?aioe iiioeyoe?e iie?ia??on?a iathoue ??cia cia/aiiy a iaoiaaiac? oa ?ioiiaaiac? iie?ii??e?oii? ?ioaeoe??. Aa??aio Aaaio+ ? a?eueo iae?ia??oeaioiei, aioeaaiiei a?aeiinii noeioeyoe?? aoii?aeueiiai oa ee?oeiiiai i?ioea??oniiai ?ioi?oaoo. Aa??aio Aaaio( ia? a?eueo ae?aaeaio ?ioiinoi?aneaio ae?th a?aeiinii oi?ioaaiiy ?ioiieo ?aaeoe?e aei aaoa?ieia?/ieo aioeaai?a. AEeaa iie?ii??e?oia(i?aeueia) aaeoeeia (AEIA-IIA) oaeiae ? iiooaei?i ?ioiiiiaeoeyoi?ii, i?e aca?iiae?? c aaeoeeiith AEAeI ?icaeaa?oueny aca?iia ?ioiinoi?an?y aoii?aeueiiai i?ioea??oniiai oa aioeoiene/iiai ?ioi?oaoo. Aea/ai? iiaeeea? iaoai?cie ?ioiiiiaeoethth/eo aoaeo?a: oaeaeea i?iieeiaiiy iie?ia??on?a a oeion oa naeac?ieo- ?ioiii?aaiio?ii?ci, ooeiaeaeaiiy oeueo?ano?oeoo?e e?ioioeeo?a, ?iaeoeoe?y aiiioico, ci?ie a neioac? ?ioa?oa?iio. Aenia?eiaioaeueii aeiaaaeaii, ui cieaeaiiy aioeo?eiooai?aiiy i?e iaeii/ani?e ?ioi?caoe?? aaeoeeiaie AEIA oa AEAeI iiaea aooe iaeo?ae?ciaaia i?e ?o ?ic”?aeiaiiio aaaaeaii? (iioeiaeueiee oa?i?i- 21 aeiaa). Aoaeoeai?noue cai?iiiiiaaiiai oaeineiiaeaiiy iaoiaeieia?? ?ioiii?io?eaeoeee iie?ii??e?oo, aeeooa??? oa i?aaoeth i?aeoaa?aeaeaia i?e aaeoeeiaoe?? ae?oae. Eeth/ia? neiaa: iie?ii??e?o, ?ioiiiaoiaaiac, aaeoeeii? oa a??oeaioi? a??one iie?ii??e?oo, iiioeyoe?? iie?ia??on?a, aaioii?o, aeenioe?aoe?y, aa??aioe Aaaio+ oa Aaaio(, ?ioiiiiaeoeyoe?y, iie?ii??e?oi? aaeoeeie. Kornushenko O.N. Immunopathogenesis of experimental poliomyelitis from positions of heterogeneity populations polioviruses.- The manuscript. The thesis for a doctor’s degree of biological sciences by speciality 03.00.06-virology. Bogomolets National Medical University, Kiev, 1999. The dissertation is devoted to research of immunobiological properties of variants heterogeneous populations vaccine and virulent poliomyelitis viruses. Is shown, that in process reproduction polioviruses dissociation on variants (Abent+ and Abent() with different affinity to natural sorbent bentonit and genotypes characteristics: virulent, antigenic, immunogenic, immunoorganotropism, immunomodulation properties. Dissociants of polioviruses populations have different meaning in pathogenesis and immunogenesis of an poliomyelitis infection. The variant Abent+ is more neurovirulent, antigenic rather stimulation of hymoral and cell antiviruses immunity. The variant Abent( has more expressed immunosupressive action concerning formation of immune reactions to heterologycal antigenes. Alive or oral poliomyelitis vaccine (APV-OPV) also is strong immunomodulation, with interaction with a vaccine DTP develops immunosupression hymoral antiviruses and antitoxic immunity. The possible mechanisms of immunomodulation effects are investigated: fast penetration of polioviruses in thymus and spleen- immunoorganotropism, damage of ultrastructure lymphocytes, induction apoptosis, change in synthesis interferon. Experimentally is proved, that the decrease of antibody-producing with simultaneous immunisation by vaccines APV (OPV) and DTP can be neutralized with their separate introduction (optimum time 21 days). The efficiency of the offered improvement immunoprophylactic of poliomyelitis, diphtheria and tetanusis confirmed with of immunisation children. Key words: poliomyelitis, immunopathogenesis, vaccine and virulent poliomyelitis viruses, population of polioviruses, bentonit, dissociation, variants Abent+ and Abent(, imunomodulation, poliovirus vaccine. Ei?ithoaiei I.I. Eiioiiiaoiaaiac yenia?eiaioaeueiiai iieeiieaeeoa n iiceoeee aaoa?iaaiiinoe iiioeyoeee iieeiae?onia.- ?oeiienue. Aeenna?oaoeey ia nieneaiea o/aiie noaiaie aeieoi?a aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.06 - ae?onieiaey.- Iaoeeiiaeueiue iaaeeoeeineee oieaa?neoao ei.A.A.Aiaiiieueoea, Eeaa, 1999. Aeenna?oaoeey iinayuaia eco/aieth aaeoeeiiuo e ae?oeaioiuo ae?onia iieeiieaeeoa, ia o?iaia eo aaoa?iaaiiuo iiioeyoeee. ?aaioa ioiineony e oai?aoe/aneie ae?onieiaee, a iae iaoee iiaeoaa?aeaeaiea e ?acaeoea eaeae i?ioanni?a A.I.Oe?iaieiaa, eioi?ue ioe?ue yaeaiea aeennioeeaoeee yioa?iae?onia e eco/ee aai aeey ae?onia Eienaee. Aeaaiue i?eioeei aeennioeeaoeee ninoieo a oii, /oi a i?ioeanna ?ai?iaeoeoeee a aaoa?iaaiiuo ii naiae i?e?iaea iiioeyoeeyo, yioa?iae?onu ?anuaieythony ia aaiaoe/aneea aa?eaiou n ?acee/iie aeieiae/aneie aeoeaiinoueth e oeceei-oeie/aneeie oa?aeoa?enoeeaie. Aeey naeaeoeee aa?eaioia ec enoiaeiuo iiioeyoeee yioa?iae?onia eniieueciaaee aaeue i?e?iaeiiai ieia?aea aaioiieoa, eioi?ue eiaao aunieoth ni?aoeeiiioth niiniaiinoue. Yioa?iae?onu eiatho ?acee/iue aooeieoao e aaioiieoo: iaeie aa?eaiou iiioeyoeee oa?aeoa?ecothony aunieie aaeni?aoeeiiiie niiniaiinoueth e ieia?aeo, /anoi iie aeiieie?otho a ninoaaa iiioeyoeee, eo iaicia/atho neiaieii Aaaio+. Niioaaonoaaiii, ieii?iay /anoue iiioeyoeee n ieceei aooeieoaoii e aaioiieoo auea iacaaia Aaaio( . Aeennioeeaoeey yioa?iae?onia i?ienoiaeeo a i?e?iaea, a i?aaiecia /aeiaaea, a eaai?aoi?iuo oneiaeyo, anoanoaaiii, /oi aeeaay iieeiieaeeoiay aaeoeeia-AEIA, ia iiaeao auoue eneeth/aieai. Aooaioe?iaaiiua ooaiiiu Nyaeia a i?ioeanna eo iinoiyiiie oee?eoeyoeee a i?aaiecia aaeoeeie?iaaiiuo, eiioaeoiuo n ieie, a ie?oaeathuae n?aaea, aeennioee?otho ia aaiaoe/aneea aa?eaiou, eioi?ua iiaoo ii ?aciiio aeeyoue ia i?ioeann eiioiiiaoiaaiaca. Yoa i?aaeiinueea auea aaaeouae aeey i?iaaaeaiey enneaaeiaaiee e ii?aaeaeeea aeaaioth caaea/o: eco/aiea nayce aaoa?iaaiiinoe iiioeyoeee ae?onia iieeiieaeeoa n eo eiioiiiiaeoee?othuei aeaenoaeai. A yenia?eiaioaeueiuo enneaaeiaaieyo, i?iaaaeaiiuo ia iiaeaeyo eoeueoo? eeaoie e eeiaeiuo iuoao auei iieacaii, /oi iieeiae?onu caeiiiia?ii aeennioeee?otho ia aa?eaiou, a ninoaaa iiioeyoeee iieeiae?onia ?? e ??? oeiia aeiieie?otho aa?eaiou Aaaio+ , a ae?oeaioiue ooaii Mahoney ? oeia i?aeoe/anee ninoieo ec “ieioniaiai” aa?eaioa. Aa?eaiou ?acee/athony ii eiioiiaeieiae/aneei oa?aeoa?enoeeai, aaaeouay ?ieue a iaoiaaiaca yenia?eiaioaeueiiai iieeiieaeeoa, a eiaeoeoeee aoii?aeueiiai e eeaoi/iiai i?ioeaiae?oniiai eiioieoaoa i?eiaaeeaaeeo aa?eaioai Aaaio+. Aa?eaiou Aaaio( yaeythony eiioiinoi?anneaiuie, eciaiytho eiioiiue ioaao e aaoa?ieiae/iui aioeaaiai - y?eo?ioeeoai, oeiioeeoai, iaoiaaiiui noaoeeieieeai, eiiiiiaioai aaeoeeiu AEAeN - aeeooa?eeiiio e noieaiy/iiio aiaoieneiai. Yoio oaiiiai iiaeao i?iyaeyoueny eae a aeaea eiioiinoeioeyoeee, oae e eiioiinoi?annnee aoii?aeueiuo e eeaoi/iuo ?aaeoeee Aoaeouaa oniaa?oainoaiaaiea aaeoeeiu AEIA iiaeao auoue naycaii n inaiaiaeaeaieai aa iiioeyoeee io eiiiiiaioa Aaaio( n au?aaeaiiui eiioiinoi?anneneaiui aeaenoaeai. A oneiaeyo yenia?eiaioaeueiuo iiaeaeae oaeaea auei eco/aii aeeyiea aaiaoe/aneeo aa?eaioia iiioeyoeee aaeoeeiiuo iieeiae?onia ia aoii?aeueiue eiioieoao e aeeooa?eeiiio e noieaiy/iiio aiaoieneiai - eiiiiiaioai aaeoeeiu AEAeN. Iieacaii, /oi ana o?e ooaiia Nyaeia eiatho /aoei au?aaeaiiua eiioiinoi?anneaiua naienoaa ii ioiioaieth e ia?aciaaieth aioeoieneiia, noaiaiue eo ?aaeecaoeee auea ?acee/iie. A aieueoae ia?a no?aaeaao eiioiiue ioaao e aeeooa?eeiiio aiaoieneio, /ai e noieaiy/iiio Ia iiaeaee aaeoeeiaeueiiai i?ioeanna o iuoae auei i?iaaaeaii yenia?eiaioaeueiia eco/aiea acaeiiiai eiioiiiiaeoee?othuaai aeaenoaey AEIA, EIA ( eiaeoeae?iaaiiie aaeoeeiu) ioiineoaeueii aioeoaeiia?aciaaiea e eiiiiiaioai aaeoeeiu AEAeN, e iaiai?io, ii?aaeaeaii aicaeaenoaea AEAeN ia oi?ie?iaaiea i?ioeaiae?oniiai eiioieoaoa.. Eiioiiiiaeoee?othuee yooaeo AEIA caeeth/aaony a au?aaeaiiie eiioiinoi?annee ioiineoaeueii ia?aciaaiey aeeooa?eeiuo e noieaiy/iuo aioeoieneiia, oeo?u i?ioeaiaeeooa?eeiuo aioeoae oiaiueoathony iiae aicaeaenoaeai AEIA a 12,44 ?aca, a noieaiy/iuo – a 16 ?ac. I/aaeaeii ?aaeecaoeey oaeiai eiioiiiiaeoee?othuaai yooaeoa i?ienoiaeeo a oneiaeyo iaeiia?aiaiiie eiioiecaoeee aeeaie iieeiieaeeoiie aaeoeeiie e AEAeN. EIA eiaao na?ueaciua i?aeiouanoaa a ieaia eiiaeiaoeee n AEAeN, eiioiinoi?anneaiua naienoaa o yoie aaeoeeiu i?aeoe/anee ionoonoaotho. Eco/aiu aiciiaeiua iaoaieciu eiioiiiiaeoee?othuaai aeaenoaey iieeiae?onia e aaeoeei AEIA, EIA, AEAeN a ?aciuo aa?eaioao aaaaeaiey, e iei ioianaiu: eiioiii?aaiio?iieci aaeoeeiiuo iieeiae?onia, iia?aaeaeaiea oeueo?ano?oeoo?u eeioioeeoia naeacaiee e oeiona, eiaeoeoeey aiiioica eeioioeeoia, eciaiaiey neioaca eioa?oa?iia. Iaeo?aeecaoeey acaeiiiai eiioiinoi?anneaiiai aeaenoaey i?ienoiaeeo i?e ?acuaaeeiaiee i?ioeanna eiioiecaoeee ai a?aiaie, iioeiaeueiue n?ie – 21 aeaiue.Yooaeoeaiinoue oaeiai ?acaeaeueiiai iaoiaea eiioiecaoeee aaeoeeiaie AEIA e AEAeN iiaeoaa?aeaeaia ia ia?aie/aiiii eiioeiaaioa aeaoae. Iaaethaeaee iiauoaiea oeo?ia ae?oniaeo?aeecothueo aioeoae ei anai oeiai iieeiae?onia, oiaiueoaiea ia 43,05 % eiee/anoaa aeaoae aac i?ioaeoeaiiai oeo?a aeey aeeooa?ee. Eeth/aaua neiaa: iieeiieaeeo, eiioiiiaoiaaiac, aaeoeeiiua e ae?oeaioiua ae?onu iieeiieaeeoa, iiioeyoeee iieeiae?onia, aaioiieo, aeennioeeaoeey, aa?eaiou Aaaio+ e Aaaio(, eiioiiiiaeoeyoeey, iieeiieaeeoiua aaeoeeiu. PAGE 26 PAGE 40

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