3

I?i?noa?noai ioi?iie caei?ia`y Oe?a?ie

Ee?anueeee iaoeiai-aeine?aeiee ?inoeooo ioiea?eiaieia??

?i. i?io. I. N. Eieii?e/aiea

Eae?ca Na?a?e Ieaen?eiae/

OAeE: 616.322-002.2:615.37

?ioiia?ieia?/ia ae?y ae?ae?caaaeueieo

/eiiee?a ?c E?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee
oiicee?o

14.01.19 — ioi?eiiea?eiaieia?y

Aaoi?aoa?ao

aeena?oaoe?? ia caeiaoooy iaoeiaiai

nooiaiy eaiaeeaeaoa iaaee/ieo iaoe

Ee?a – 1998

Aeena?oaoe??th ? ?oeiien.

?iaioa aeeiiaia a Ee?anueeiio iaoeiai-aeine?aeiiio ?inoeooo?
ioiea?eiaieia?? ?i. i?io. I. N. Eieii?e/aiea.

Iaoeiaee ea??aiee:

caneoaeaiee ae?y/ iaoee oa oaoi?ee Oe?a?ie, aeieoi? iaaee/ieo iaoe,
i?ioani? Caaieioiee Aeieo?i ?ee?/, aee?aeoi? Ee?anueeiai
iaoeiai-aeine?aeiiai ?inoeoooo ioiea?eiaieia?? ?i. i?io.
I.N.Eieii?e/aiea.

Iaoeiaee eiinoeueoaio:

aeieoi? iaaee/ieo iaoe, i?ioani? Iaeueieeia Ieaa Oaiaein?eiae/,
caa?aeoaa/ eaai?aoi??? iaoio?c?ieia?? oa ?ioiieia?? Ee?anueeiai IAe?
ioiea?eiaieia?? ?i. i?io. I.N.Eieii?e/aiea.

Io?oe?ei? iiiiaioe:

aeieoi? iaaee/ieo iaoe, i?ioani? O?eaoia A?eoi? Oiie/, caa?aeoaa/
eaoaae?e ioiea?eiaieia?? Oa?e?anueeiai iaaee/iiai oi?aa?neoaoo;

aeieoi? iaaee/ieo iaoe, i?ioani? Aethi?i Ieaa Aaea??eiae/, caa?aeoaa/
eaoaae?e ioi?eiiea?eiaieia?? Iaeanueeiai aea?aeaaiiai iaaee/iiai
oi?aa?neoaoo.

I?ia?aeia onoaiiaa: Ee?anueea aea?aeaaia iaaee/ia aeaaeai?y
i?neyaeeieiiii? ina?oe ?i. I.E.Ooieea, eaoaae?a ioi?eiiea?eiaieia??, IIC
Oe?a?ie, i.Ee?a.

Caoeno aeena?oaoe?? a?aeaoaeaoueny 16_ a?oaeiy 1998 ?. i 12 aiaeei? ia
can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae 26.611.01 Ee?anueeiai
iaoeiai-aeine?aeiiai ?inoeoooo ioiea?eiaieia?? ?i. i?io. I.N.
Eieii?e/aiea IIC Oe?a?ie (252057, Ee?a aoe.Ciieia?/ia, 3)

C aeena?oaoe??th iiaeia iciaeiieoeny a a?ae?ioaoe? Ee?anueeiai iaoeiai-
aeine?aeiiai ?inoeoooo ioiea?eiaieia?? ?i. i?io. I.N.Eieii?e/aiea, IIC
Oe?a?ie (i.Ee?a, aoe. Ciieia?/ia, 3)

Aaoi?aoa?ao ?ic?neaii «_16_»_eenoiiaaea_ 1998 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee

eaiaeeaeao iaaee/ieo iaoe A.?. ?iceeaaeea

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue oaie

Iacaia?a/i? aeieace o/ano? oiicee a ?aaeoe?yo ?ioi?oaoo, i?aaenoaaeai?
ca inoaii? 20 ?ie?a, ni?eyee a?eueo no?eiaiiio i?aeoiaeo aei
iia?aoeaiiai e?eoaaiy o?ii?/iiai oiicee?oo ? ?ic?iaoe? iaoiae?a
eiina?aaoeaii? oa?ai?? oeueiai caoai?thaaiiy, ui aacothoueny ia
i?eioeeiao eieaeueii? oa nenoaiii? ?ioiiiiaeoeyoe??
(A.N.I?aia?aaeaineee, A.I.Iiiiaa, 1970; Y.A.Atheeeia, I.O.Iaeueieeia,
1976; A.A.Aa?oeai?a, 1978; A.I.Ai?aa/aaneee, 1980; A.E.Oeuaaiia e
niaao., 1980; I.O.Iaeueieeia, 1981; A.A.Iiia, 1984; I.A.?ueueneay, 1990;
A.A.*a?ieoia, 1991; Ae.E.Oa?ania, A.E.Naaaeiae/, 1995; A.A.E?uoe, 1996;
Ae.?.Caaieioiee, 1997; A.O.O?eaoia oa ni?aaao., 1997; A.I.O?ieia oa
ni?aaao., 1997; I.A.Aethi?i, 1998; Surian, 1997; Okada et. al. 1986;
Hirao et. al.,1996).

Iaeaaei?noue oiicee aei i?aai?a ?ioi?oaoo i?eaaea aei /eneaiieo ni?ia
aeae?eaiiy ?c ieo a?ieia?/ii-aeoeaieo ?a/iaei iie?iaioeaeii? oa
a?eeiai-iaioeaeii? i?e?iaee (Y.A.Eoea/, 1974; A.O.Oaaeinii e niaao.,
1982; Falk, 1960; Roklin et. al, 1973; Schroeder, 1972) ye?, iaeiae, ia
io?eiaee iioe?aiiy ye ?ioiiiiaeoeyoi?e aeey ee?i?/iiai canoinoaaiiy.
?icaeoie iieaeoey?ii? ?ioiieia??, cie?aia, ?ice?eooy no?oeoo?e ? ?ie?
/eneaiieo oeeoie?i?a, ye? i?iaeoeothoueny e?ioi?aeieie oa iae?ioi?aeieie
ee?oeiaie, aeicaieea ia o?eueee ?icoeo?oaaoe iaoai?cie i?aeee?oeiii?
aca?iiae??, aea e aeei?enoaoe oe? ?a/iaeie o aeaeyae? ?ioiiiiaeoeyoi??a
ae?oaiai iieie?iiy (I.A.Iaaeoieoeei, 1980; N.I. Ayae?i, I.I. Iaaaoei,
1989; A.N.Ooaoaa, 1989, E.A.Eiaaeue/oe, E.A.Aaiueeianeay, 1995; Smith,
1988).

Aea/aiiy oeeoie?iiaiai niaeo?o i?aeiaa?iieo ieaaeaeee?a i?e o?ii?/iiio
oiicee?o? iieacaei, ui a ieo ia? i?noea aeao?oeeo ?yaeo oaeoi??a,
iaiao?aeieo aeey oi?ioaaiiy iiaiioe?iii? ?ioiii? a?aeiia?ae? (Murakata
et. Al., 1996; Tanaka et. al., 1996). Oea i?aeoaa?aeaeo?oueny io?eiaieie
?ai?o aeaieie i?i ?icaeoie a ieaaeaeeeao i?e o?ii?/iiio oiicee?o?
eieaeueii? ?ioiieia?/ii? iaaeinoaoiino? (I.O.Iaeueieeia, 1981;
A.Ae.sseiaaiei, 1985). Ii?yae ?c oeei a?aei?/aii caeaoi?noue
i?aeiaa?iieo ieaaeaeee?a aei i?iaeoeoe?? oaeoi?o iae?ico iooeei (OII),
?ioa?oa?iio (?IO), ui na?ae/eoue i?i ?o aeoeaio ?ioiieia?/io ooieoe?th.
Oea ocaiaeaeo?oueny c aeaieie i?i ae?aaeaiee aeoeaoth/ee aieea ia
e?ioi?aei? ooai?aiiy eeoeiaiai o?aeoo ieoaiaioeaeo, io?eiaiiai ?c oeaiei
oiicee (I.I.Oiaeueieoeeay, 1991).

Oaeei /eiii, aeae?eaiiy ?c i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee
oiicee?o ?ioiiaeoeaieo noanoaioe?e aeicaieeoue ?icoeo?oaaoe iaoai?cie
caoenii? ooieoe?? oiicee, aa??aioe ?o ii?ooaiiy, a oaeiae io?eiaoe
?ioiiaeoeai? oaeoi?e, ye? iiaeooue aooe iioaioe?eieie e?eoaaeueieie
caniaaie iiaiai iieie?iiy.

Ca’ycie c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie

Aeena?oaoe?y ? neeaaeiaith /anoeiith iaoeiai-aeine?aeieo oai Ee?anueeiai
IAe? ioiea?eiaieia?? ?i. i?io. I.N.Eieiiee/aiei IIC Oe?a?ie: “?ic?iaea
iaoiae?a iaea?aeaiiy aeiaaiiiai oa enaiiaaiiiai “oaeoi?o ia?aiino” oa
canoinoaaiiy ?o aeey e?eoaaiiy ?ioiiaeao?oeeo?a, o?ii?/ieo ?ioaeoe?e oa
ceiye?nieo iooeei”, ? aea?ae?a?no?aoe?? 0193U039491; “Aeine?aeaeaiiy
i?ioeiooeeiii? ae?? ?ic/eiiiai oaeoi?o(?a) ?c oeaieiieo e?ioioeeo?a”, ?
aea?ae?a?no?aoe?? 0196U010082.

Iaoa ?iaioe

A?aoiaoth/e aeeeaaeaia, iaoith ?iaioe aoei aeae?eaiiy, a?io?i?/ia oa
?ioiieia?/ia oa?aeoa?enoeea ?ioiiaeoeaieo ae?ae?caaaeueieo /eiiee?a ?c
ee?oei i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o.

Caaea/? aeine?aeaeaiiy

1. Aecia/eoe iioeiaeueiee ?aaeei aeae?eaiiy ?c ee?oei i?aeiaa?iieo
ieaaeaeee?a ae?ae?caaaeueieo /eiiee?a.

2. Aeaoe a?io?i?/io oa?aeoa?enoeeo io?eiaieo ae?ae?cao?a.

3. I?iaanoe aeine?aeaeaiiy ii aecia/aiith ?ioiiiiaeoethth/eo
aeanoeainoae ae?ae?cao?a ?c ee?oei i?aeiaa?iieo ieaaeaeee?a.

4. Aeine?aeeoe aieea ae?ae?caaaeueieo oaeoi??a ?c e?ioioeeo?a
i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o ia aeaye?
iaoai?cie i?ioeiooeeiii? ?acenoaioiino?.

I?e i?iaaaeaii? aeine?aeaeaiue aeei?enoiaoaaee eiiieaen a?io?i?/ieo,
?ioiieia?/ieo, ee?i?/ieo ? noaoenoe/ieo iaoiae?a aeine?aeaeaiiy.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a

1. ?c ee?oei i?aeiaa?iieo ieaaeaeee?a iiaeeo?eiaaiei iaoiaeii io?eiaii
ae?ae?caaaeueiee oaeoi?/?e (AeO) ?c iieaeoey?iith ianith <25 eAe c oe?ieei niaeo?ii ?ioiiiiaeoethth/eo aieea?a. 2. Ae?ae?caaaeueiee /eiiee i?noeoue iecueeiiieaeoey?i? a?eee, i?ioaie?oe/i? oa?iaioe oa ia? aioei?ioaaci? aeanoeaino?. 3. ?ioiiiiaeoth/? aeanoeaino? ae?ae?caoo i?iyaeythoueny a eiai caeaoiino? ia?aiineoe a?ia?/ooeea?noue niia?eueiaii? ae?? (ANAe) aei no?aioie?ceio-I (No-O), iineethaaoe a aeae?eueea ?ac?a aoi?eiio aoii?aeueio ?ioiio a?aeiia?aeue aei oeueiai aioeaaio oa ca?eueooaaoe in vitro e?euee?noue O-e?ioioeeo?a, ye? aeni?anothoue ND-2-?aoeaioi?e. 4. Aeo?aeiee ae?ae?cao ia ia? aeanoeainoae ?IO, iaeiae ia? aeoeai?noue OII, yea c?inoa? i?e eoeueoeaoaaii? e?ia? ?c ae?ae?caoii. 5. Ae?ae?cao caeaoai aeoeaoaaoe iaoai?cie i?ioeiooeeiii? ?acenoaioiino? – aaeueioaaoe ?icaeoie iooeeiiiai i?ioeano o ieoae i?e ia?auaieai? ?i ea?oeeiiie Eueth?n, aeoea?coaaoe i?e?iaei? e?ea?i? ee?oeie (IE-ee?oeie) oai?eo ia ?ae ai?oai?, a oaeiae aeeeeeaoe e?cen ee?oei Hep-2, K-562, aea ia aeiaaiieo e?ioioeeo?a oiicee ? e?ia?. I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a 1. ?ic?iaeaii oaoiieia?th eaai?aoi?iiai io?eiaiiy ae?ae?caaaeueiiai oaeoi?o ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o, yea iiaea aooe iniiaith aeey ?ic?iaee i?iieneiai? oaoiieia?? ae?iaieoeoaa e?eoaaeueiiai i?aia?aoo. 2. Io?eiai? a oiae? aeine?aeaeaiue aeai? na?ae/aoue i?i oa, ui a ieaaeaeeeao, iaa?oue i?e o?ii?/iiio caiaeaii?, i?noyoueny ?ioiiaeoeai? ?a/iaeie, ye? a?ae?a?athoue aaaeeeao ?ieue a caaacia/aii? aiiainoaco, ui ia?aaeaa/a? a?eueo aeeoa?aioe?eiaaiee i?aeo?ae o ?ic?iaoe? iieacaiue aei iia?aoeaiiai e?eoaaiiy o?ii?/iiai oiicee?oo. Ai?iaaaeaeaiiy o i?aeoeeo ?ic?iaeaio oaoiieia?th eaai?aoi?iiai io?eiaiiy ae?ae?caaaeueiiai oaeoi?a ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o ai?iaaaeaeaii o i?aeoeeo eaai?aoi??? iaoio?c?ieia?? oa ?ioiieia??, a oaeiae eaai?aoi??? a?io?i?? Ee?anueeiai IAe? ioiea?eiaieia??. ?aeiiaiaeo?oueny io?eiai? aeai? aeei?enoiaoaaoe a eo?n? eaeoe?e c o?c?ieia?? e?ioiaeioi/iiai e?eueoey. Iniaenoee aianie caeiaoaa/a Aaoi?o iaeaaeeoue ?aeay oaoiieia?? aenia?eiaioaeueieo aeine?aeaeaiue in vitro oa in vivo. Aenia?eiaioaeuei? aeine?aeaeaiiy ia oaa?eiao aeeiiai? naiino?eii, ?ioiieia?/i? aeine?aeaeaiiy – ni?eueii c? ni?a?ia?oieeaie eaai?aoi??? iaoio?c?ieia?? oa ?ioiieia?? Ee?anueeiai IAe? ioiea?eiaieia??, a?io?i?/i? – ni?eueii c? ni?a?ia?oieeaie eaai?aoi??? a?io?i?? EIAe?IE. Ianoaaeaiiy oai?eo oa caa?? ee?i?/iiai iaoa??aeo aeeiiai? aaoi?ii naiino?eii. Ai?iaaoe?y ?acoeueoao?a aeena?oaoe?? Iaoa??aee aeena?oaoe?? aeiiia?aeaeeny ia IV-io c`?cae? ioiea?eiaieia?a ?anioae?ee A?ei?onue (A?oaanuee, 1996); II Iaoe?iiaeueiiio eiia?an? iaoio?c?ieia?a Oe?a?ie “Aeooaeuei? ieoaiiy ee?i?/ii? ?ioiieia??” (Ee?a, 1996); iaoeia?e eiioa?aioe??, i?enay/ai?e 100-??//th c aeiy ia?iaeaeaiiy i?io. I.N.Eieii?e/aiea (Ee?a, 1998 ?.). Ai?iaaoe?eiee caoeno aeena?oaoe?? a?aeaoany 28 aeiaoiy 1997 ?ieo ia can?aeaii? A/aii? ?aaee Ee?anueeiai IAe? ioiea?eiaieia?? ?i. i?io. I.N.Eieii?e/aiea. Ioae?eaoe??. Ii oai? aeena?oaoe?? iioae?eiaaii 4 aeo?iaeuei? noaoo?, 2 – a ca??eao iaoeiaeo i?aoeue. No?oeoo?a aeena?oaoe??. Aeena?oaoe?y aeeeaaeaia ia 118 noi??ieao iaoeiiieniiai oaenoo ? neeaaea?oueny ?c anooio, iaeyaeo e?oa?aoo?e, /ioe?ueio ?icae?e?a aeanieo aeine?aeaeaiue, caeeth/aiiy, aeniiae?a, i?aeoe/ieo ?aeiiaiaeaoe?e, nieneo aeei?enoaii? e?oa?aoo?e, yeee iae?/o? 155 aeaea?ae, c ieo 79 – ?iicaiieo aaoi??a. ?iaioo ?ethno?iaaii 23 oaaeeoeyie oa 6 ?enoieaie. CI?NO ?IAIOE Iaoa??aee oa iaoiaee aeine?aeaeaiue Iaoa??aeii aeey aeine?aeaeaiue neoaeeee i?aeiaa?ii? ieaaeaeeee oa ia?eoa?e/ia e?ia oai?eo ia o?ii?/iee oiicee?o, ia?eoa?e/ia e?ia i?aeoe/ii caei?iaeo in?a, e?ioi?aei? i?aaie ? ia?eoa?e/ia e?ia ieoae e?i?e NAA; C57Bl/6, iae?i?eieo ieoae oa uo??a e?i?? Wistar. E??i oiai, iaoa??aeii aeey aeine?aeaeaiue aoee eoeueoo?e ee?oei ??ciiai a?noiaaiaco: L-41, iooeeii? e?i?? (E-562; Ia?-2), o?a?iaeanoe (L-929), e?ioioeeoe aeiaaiieo ieaaeaeee?a oa ia?eoa?e/ii? e?ia? oai?eo ?aeii ai?oai? ? iaoe??io?a ?c caiaeueieie i?ioeanaie aa?oi?o aeeoaeueieo oeyo?a, a?eo?ioeeoe ethaeeie a?oie AA. Iaoa??ae aeey aeine?aeaeaiue io?eiaiee a?ae 66 oai?eo, a oiio /ene? ia o?ii?/iee oiicee?o (32); ?ae ai?oai? (20); e?ioina?eiio aa?oi?o aeeoaeueieo oeyo?a (14), a oaeiae a?ae 10 i?aeoe/ii caei?iaeo in?a. Ie??i oiai, a ?iaio? aeei?enoaii 348 ieoae e?i?e NAA, C57Bl/6, a?eeo iae?i?eieo ieoae oa 46 uo??a Wistar. Aeey iaea?aeaiiy oa aeine?aeaeaiiy ?ioiiaeoeaieo noanoaioe?e a ae?ae?caaaeueieo o?aeoe?yo oeaieiieo e?ioioeeo?a aeei?enoai? ee?oeie i?aeiaa?iieo ieaaeaeee?a, a oaeiae aeine?aeaeoaaeanue ia?eoa?e/ia e?ia oai?eo ia o?ii?/iee oiicee?o, eio?ei ca iieacaiiyie aoea i?iaaaeaia oiiceeaeoii?y. Aeiii?aie e?ioioeeo?a ia?eoa?e/ii? e?ia? aoee oaeiae i?aeoe/ii caei?ia? iniae, ui i?ioiaeeee ?ioiieia?/ia ianoaaeaiiy a eaai?aoi??? iaoio?c?ieia?? oa ?ioiieia?? Ee?anueeiai IAe? ioiea?eiaieia??. Aeiii?e i?aeiaa?iieo ieaaeaeee?a i?aaenoaaeai? oai?eie c ae?aaiicii: o?ii?/iee oiicee?o, aeaeiiiainiaaia oi?ia, ?aoeeaeeae aia?i (E.A.Nieaeaoia, 1975). A ?iaio? aeei?enoai? i?aeiaa?ii? ieaaeaeeee 20 /ieia?e?a oa 12 ae?iie a?eii a?ae 16 aei 40 ?ie?a ?c aeaai?noth caoai?thaaiiy ia iaioa 3 ?ie?a. I?e aeine?aeaeaii? ?ioiiaeoeaieo aeanoeainoae ae?ae?caaaeueieo o?aeoe?e ?c e?ioioeeo?a ieaaeaeee?a ? ia?eoa?e/ii? e?ia? aeei?enoiaoaaee ee?oeie e?ia? oai?eo ia ?ae ai?oai?, ye i?aaeei, II-III noaae?? ae?oai? ee?i?/ii? a?oie, ye? iaee a?aeoeeaiiy a noai? ?ioiii? nenoaie ia iiiaio ianoaaeaiiy, a oaeiae oai?eo ia e?ioina?eiio aa?oi?o aeeoaeueieo oeyo?a. Aenia?eiaioaeuei? aeine?aeaeaiiy aeeiiai? ia iae?i?eieo ieoao, ieoao e?i?e NAA, C57Bl/6, BALB/c oa uo?ao Wistar. E??i oiai, /anoeia aenia?eiaio?a aeeiiaia ia eoeueoo?ao ee?oei, oaeeo ye L-929, L-41 (?IAA IAI Oe?a?ie), Hep-2 ? K-562 (OIOe?I). Ooai ee?oei ea?oeeiiie Eueth?n io?eiaiee ?c ?AII? IAI Oe?a?ie. Oaa?eie oo?eioaaeeny a oiiaao a?aa??th EIAe? ioiea?eiaieia??. ?iaioa c ieie i?iaiaeeeany o a?aeiia?aeiino? c i?aaeeaie iiaiaeaeaiiy ?c aenia?eiaioaeueieie oaa?eiaie. A aenia?eiaioao in vitro aeei?enoiaoaaeenue oaeiae ei?ioeioa?i?iia? eoeueoo?e aeiaaiieo oiiceeioeeo?a, enaiiaaiieo (a?eo?ioeeoe eo?ae) ? aeiaaiieo a?eo?ioeeo?a, ia yeeo ia?aa??yeanue oeeoioiene/ia ae?y iaea?aeaieo ae?ae?caaaeueieo o?aeoe?e. Eoeueoo?e aiooaaee a aeaiue iinoaiiaee aenia?eiaioo. Iaoiaee a?io?i?/iiai ?icae?eaiiy oa aiae?co Io?eiaiiy o?aeoe?e ?ic/eiieo ae?ae?caaaeueieo oaeoi??a ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia aeaeiiiainiaaio oi?io o?ii?/iiai oiicee?oo ? ia?eoa?e/ii? e?ia? ethaeae cae?enithaaee o a?aeiia?aeiino? c ?aeiiaiaeaoe?yie O?ueiaea?a, Ei?aioea (1979), c iaoeie iiaeeo?eaoe?yie, iienaieie o ?icae?e? aeanieo aeine?aeaeaiue. Aecia/aiiy caaaeueiiai ai?noo a?eeo a ae?ae?caoao i?iaiaeeee ca iaoiaeii Eio?? (1951). Coia?io i?ioaie?oe/io aeoeai?noue aeyaeyee ca iaoiaeii E.I.Aa?ai??iei ? ni?aaoi??a (1988). Aeey aeyaeaiiy o?eineiiiiae?aii? aeoeaiino? aeei?enoiaoaaee o?iiiaaiiee noano?ao N-aaici?e-AeL-a?aei?i-i-i?o?iai?e?ae-AAIIA (E.I.Aa?ai??iei, 1971). Aeoeai?noue o?eineiiiiae?aiiai oa?iaioo eae?e?a?io aeine?aeaeoaaee ca aeiiiiiaith niaoeeo?/iiai o?iiiaaiiiai noano?aoo – o?iiiceio ?E (Ialmark, Davie, 1984). ??aaiue e?i?i?oeioth/iai oa?iaioo e?i?iace aecia/aee ca oaeaee?noth ?icuaieaiiy noano?aoo a?io?ee-L-e?ceio (Folk et. al., 1960). Caaaeueio aioeo?eioe/io aeoeai?noue aeine?aeaeoaaee ca iaoiaeii E.I.Aa?ai??iei ? ni?aaaoi??a (1988). ?icae?eaiiy a?ee?a ae?ae?caaaeueii? o?aeoe?? i?iaiaeeee iaoiaeii aeene-aeaeo?ioi?aco, aeei?enoiaoth/e 15 % iie?ae??eai?aeiee aaeue ?c aeiaeaoeeenoeueoaoii iao??th (Tuemml, 1970). ?ioiieia?/i? iaoiaee I?enooi?noue oeeoie?i?a o ae?ae?caoao ?c c?oeiiaaieo ee?oei i?aeiaa?iieo ieaaeaeee?a ? noia?iaoaioao, io?eiaieo i?ney eoeueoeaoaaiiy ee?oei ia?eoa?e/ii? e?ia? ?c ae?ae?caoaie ? aac ieo, aecia/aee ca aeoeai?noth, aeanoeaith ie?aiei oeeoie?iai. Iayai?noue a aeine?aeaeaieo i?aia?aoao oaeoi?a iae?ico iooeei aeine?aeaeoaaee a eoeueoo?aeueiiio oano? ca ??aiai aeano?oeoe?? ee?oei e?i?? L-929, iiia?aaeiuei ia?iaeaieo aeoeiii?oeeiii-D (Serva), aeei?enoiaoth/e oioiiao?e/iee oano ?c e?enoae?/iei o?ieaoiaei. Oeeoioiene/iee ?iaeaen (Oe?) ?ic?aoiaoaaee ca oi?ioeith: Oe?= (a-b) 100% a aea a – iioe/ia aonoeia yiie ?c na?aaeiaeuai ? b – iioe/ia aonoeia yiie n na?aaeiaeuai oa noia?iaoaioii aai ae?ae?caaaeueiei oaeoi?ii ieaaeaeee?a (AeOI). Iioe/io aonoeio aei??thaaee i?e aeiaaeei? oaee? 540 ii. A iniia? aeaiii? iaoiaeeee eaaeaoue ?aeiiaiaeaoe?? I.N.Aey/aiei ? ni?aaaoi??a, (1994). Aeoeai?noue oaeoi?o aaeueioaaiiy i?a?aoe?? (OAI) ee?oei e?ia? aeyaeyee aeei?enoiaoth/e eai?ey?iee oano c ieai?iao?e/iith ioe?ieith ??aiy aaeueioaaiiy. A yeino? i?a?oth/eo ee?oei aeei?enoiaoaaee eaeeioeeoe e?ia? caei?iaeo aeiii??a, ye? aeae?eyee iaoiaeii niiioaiiiai inaaeaeoaaiiy ii i?ea?iaeuei?e iaoiaeeoe? (I.O.Iaeueieeia oa ni?aaao., 1985). I?e aeeiiaii? aeaiii? /anoeie ?iaioe aeei?enoiaoaaee iai?yiee iaoiae ioe?iee aaeueioaaiiy i?a?aoe?? ee?oei o a?aeiia?aeiino? ?c ?aeiiaiaeaoe?yie I.O. Iaeueieeiaa (1974). Iayai?noue a ae?ae?caoao oaeoi?o ia?aiino aecia/aee ca caeaoi?noth i?aia?aoo ia?aiineoe a?ia?/ooeea?noue niia?eueiaii? ae?? aei aioeaaio no?aioieiea-No-I o ?ioaeoieo ieoae e?i?? NAA. Ioe?ieo ?icaeoeo ANAe i?iaiaeeee ii ci?i? iane i?aeeie?iieo e?ioaoe/ieo aoce?a ca?aeii ?aeiiaiaeaoe?e A.A.Athee?iaa, I.A.Naiao? (1981). Aeoeai?noue eieii??noeioethth/iai oaeoi?o aeyaeyee a ae?ae?cao? ee?oei ieaaeaeee?a ii ??aith ? ye?ni?e oa?aeoa?enoeoe? aiaeiaaiieo eieii?e o noaeaoaeueii ii?ii?iaieo ieoae e?i?? NAA (Till, McCulloch, 1961). ?ioa?oa?ii aecia/aee ii aioea??oni?e aeoeaiino? i?aia?ao?a – ?o caeaoiino? caoeuaoe ee?oeie a?ae ca?aaeaiiy a??onii oai?iae aaceeoey?iiai noiiaoeoo (AAN). Aeei?enoiaoaaee iaoiaeeeo 50 % i?ioea??onii? caoenii? ae?? (OeIAe-50) o a?aeiia?aeiino? c ?aeiiaiaeaoe?yie I.N.Aey/aiei oa ni?aaaoi??a (1994). I?e aeine?aeaeaii? aieeao ae?ae?cao?a ia aeoeai?noue i?e?iaei?o oeeoioiene/ieo ee?oei (IOeE) i?aeoe/ii caei?iaeo in?a ? oai?eo ia ?ae ai?oai? aeei?enoiaoaaee ?aeiiaiaeaoe?? I.O.Iaeueieeiaa ? ni?aaaoi??a, (1985). Canoiniaoaaee aeaa oeie i?oaiae: ee?oeie a?eo?ieaeeico ethaeeie E-562 oa a?eo?ioeeoe eo?/ao. Ioe?ieo ?aaeoe?? i?iaiaeeee ?aae?iiao?e/ii ia onoaiiaoe? Aaiia-12 (Oe?a?ia), aeei?enoiaoth/e a yeino? ?cioiiiiai ia?ea?a Cr51 (Amersham, Aaeeeia?eoai?y). Aoaeoi?e aeae?eyee c ia?eoa?e/ii? e?ia? oeyoii ?icae?eaiiy ia a?aae??io? u?eueiino? oaeiee-aa?ia?ao?io. ??aaiue iiaeoeyoe?? aoii?aeueiiai ?ioi?oaoo aeine?aeaeoaaee a aenia?eiaioao in vivo ia ieoao e?i?? NAA. Aea/aee ?aaeoe?? nenoaie ?ioi?oaoo i?e aaaaeaii? ae?ae?cao?a, aioeaai?a ??ciiai oeio: no?aioie?ceio-I, a?eo?ioeeo?a aa?aia (AA). I?e aaaaeaii? no?aioie?ceio-I noia?io i?iaeoeoe?th aioeo?e ioe?ithaaee ca aeiiiiiaith ?aaeoe?? iaeo?ae?caoe??, i?e aaaaeaii? a?eo?ioeeo?a aa?aia – ca ?aaeoe??th aaiaaethoeiaoe?? ?c canoinoaaiiyi i?e?ioeo?aoi?a Oaea/? (I.O.Iaeueieeia, 1981). I?e aea/aii? oeeoioiene/ii? ae?? ae?ae?cao?a ?c ee?oei i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o oa ?c ee?oei e?ia?, aeei?enoiaoaaee ?aae?iiao?e/iee iaoiae ioe?iee nooiaith aeano?oeoe?? i?oaiae ?c canoinoaaiiyi Cr51 a aeaeyae? ?cioiiiiai ia?ea?a. E??i oeueiai, i?e aeei?enoaii? ?ioeo i?oaiae, iai?eeeaae ee?oei ?aeo ai?oai? ethaeeie Ia?-2, canoiniaoaaee a?oaeuei? aa?aieee c ianooiiith niaeo?ioioiiao???th. Aeine?aeaeoaaee oeeoioiene/io ae?th ae?ae?cao?a ??ciiai iioiaeaeaiiy oa noia?iaoaio?a i?ney ae?? ieie ia aeiaaii? e?ioioeeoe ieaaeaeee?a ? enaiiaaii? a?eo?ioeeoe. sse i?aaeei, aei?iaiaoaaee aeice ae?ae?cao?a ii 2,20 oa 200 iea ii a?eeo, a noia?iaoaio?a – 0,2 ie ia 2 iei i?oaiae a ia`?i? 1 ie na?aaeiaeua RPMI-1640 (Serva). *an eoeueoeaoaaiiy – 16 aiaeei i?e oaiia?aoo?? 37 iN. E?euee?noue eaeeioeeo?a ? ?o ?iciiae?e ca ie?aieie oi?iaie aeine?aeaeoaaeenue a iaceao e?ia? aenia?eiaioaeueieo oaa?ei caaaeueiii?eeiyoei iaoiaeii ?c aeei?enoaiiyi oa?aoaaiiy ca Iaiiaiaaeiii. I?e oeueiio iniaeeao oaaao caa?oaee ia ai?no aaeeeeo a?aioeiai?nieo e?ioioeeo?a, ye? ? iin?yie i?e?iaeiuei? ee?oeiii? oeeoioiene/iino? (E.I.Cae, C.A.Aooaiei, 1988; ?.Eioai, 1994). Aeiiii?aei? iaoiaee I?e aeine?aeaeaii? aieeao ae?ae?caaaeueiiai oaeoi?a ia ?icaeoie aenia?eiaioaeueiiai iooeeiiiai i?ioeano o ieoae e?i?? C57Bl/6, eiaei?e ?c 38 ieoae o ae?eyioe? noaaia aioo??oiueii`ycaai aaiaeeee 3o103 ee?oei ea?oeeiiie Eueth?n. *a?ac 6 ae?a oaa?ei ?icae?eeee ia 2 a?oie (20 oaa?ei a aenia?eiaioaeuei?e a?oi? ? 18 – a eiio?ieuei?e). Oaa?eiai aenia?eiaioaeueii? a?oie ia i?ioyc? 2-o oeaei?a aaiaeeee 0,1 ie AeO (2 iea i?aia?aoo ii a?eeo), io?eiaiiai c ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o. Oaa?eiai eiio?ieueii? a?oie ia i?ioyc? aeaio oeaei?a aaiaeeee ii 0,1 ie o?c?ieia?/iiai ?ic/eio. Noaoenoe/i? iaoiaee I?e iiaoaeia? aaaaoioaeoi?ieo aenia?eiaio?a aeei?enoiaoaaee ?aeiiaiaeaoe?? A.?.Eenaieiaa (1979). ?acoeueoaoe aeine?aeaeaiue ia?iaeai? noaoenoe/ii c aeei?enoaiiyi ia?aiao?e/ieo oa iaia?aiao?e/ieo e?eoa???a (A.A.Aoaea?, 1978). I?e noaoenoe/i?e ia?iaoe? ?acoeueoao?a oeo?oaaiiy aecia/aee aaee/eio na?aaeiueiai aaiiao?e/iiai oeo?a ? eiai iioeaee ca iaoiaeii A.E.Eaaeiniia (1968). ?acoeueoaoe aeine?aeaeaiiy oa ?o iaaiai?aiiy Aeey aeae?eaiiy ?c i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o ?ioiiaeoeaieo ?a/iaei aoei aeei?enoaii iiaeeo?eiaaiee iaoiae O?ueiaea?a-Ei?aioea. Ae?ae?caaaeueiee oaeoi? ieaaeaeee?a io?eioaaee ?c e?ioioeeo?a oiicee oeyoii ?o iaoai?/ii? aiiiaai?caoe??, na??? oeeee?a caii?iaeoaaiiy ? a?aeoaaaiiy c ianooiiith ia?iaeith AeIE-acith, aeoeaiaaiith ?iiaie iaai?th, oa ae?ae?cii e??cue oeaeioaiiao iaia?aio i?ioyaii 16 aiaeei. Onoaiiaeaii, ui AeOI ? iaioeaeii-a?eeiaith noi?ooth ?c iieaeoey?iith ianith < 25 eAe, aieiae?? aeoeai?noth aeayeeo i?ioaie?oe/ieo aicei?a. I?e aeaeo?ioi?aoe/iiio aeine?aeaeaii? a?eee AeOI ?iciiae?eyeenue ia?aaaaeii a cii? i?a?aoe?? - oa -aeiaoe?i?a. Iiae?aia oaoiieia?y io?eiaiiy ?ioiiaeoeaieo ?a/iaei, cia/iei /eiii a?ae??ciy?oueny a?ae iaoiaeee ?ioeo aaoi??a (A.A.Eoea/, 1975; A.O.Oaa?inii oa ni?aaao., 1982; Schroeder et. al., 1978). ?ioiiiiaeoethth/? aeanoeaino? ae?ae?caoo i?iyaeeenue o eiai caeaoiino? ia?aiineoe ANAe aei No-I a?ae ethaeeie aei ieoae, ui iiaea na?ae/eoe i?i iayai?noue a AeOI aeoeaiino?, iiae?aii? aei oaeoi?o ia?aiina. Iiaei ?ioiiiiaeoethth/ei aoaeoii, a?aei?iiei a?ae ?ai?oa iienaieo, ? caeaoi?noue AeOI iineethaaoe ?ioiio ?aaeoe?th ia aioeaaie no?aioieieo i?e oi?ioaaii? aoii?aeueii? a?aeiia?ae? ia No-I, eiee cai?noue ia?aaeoth/i? aeice aioeaaio aaiaeeee AeOI. Aoii?aeueia a?aeiia?aeue i?e ianooii?e ?ioi?caoe?? No-I nooo?ai ia?aaeuoaaea ??aaiue ?aaeoe??, eiee i?eioth/ith aoea ?i`?eoe?y aeacaiiai aioeaaio no?aioieieo (?en.1). A?oie oaa?ei 4 1 3 2 Ia?aaeoth/a aeica Oino. aooa? No.I AeO e?ia? AeO ieaaeaeee?a Iiaoi?ia NoI NoI NoI NoI ei'?eoe?y ?en. 1 Na?aaeiueiaaiiao?e/i? oeo?e aioeo?e a a?oiao oaa?ei i?e aaaaeaii? AeO ?c ee?oei e?ia? oa i?aeiaa?iieo ieaaeaeee?a Oaiiiai i?aeneeaiiy ia iia`ycaiee c iayai?noth a AeOI caeeoeiaeo e?eueeinoae No-I, ine?eueee a aeine?aeaeaiiyo ?aae?iiao?e/iei iaoiaeii ia aeyaeaii a?oeo?ioeeoie?oe/ii? caeaoiino? AeOI ii a?aeiioaiith aei /a?aiieo e?ia`yieo o?eaoeue ethaeeie. I?e i?iaaaeaii? iiaeaeueoeo aeine?aeaeaiue ii ?ioiieia?/i?e oa?aeoa?enoeoe? AeOI, aoea aeyaeaia eiai caeaoi?noue iineethaaoe in vitro aeni?an?th NAe-2 ?aoeaioi??a ia O-e?ioioeeoao, ui caeeaeo? eiai c i?aia?aoaie, io?eiaieie ?ioei oeyoii ?c aeei/eiai? caeice oa oiicee. Aaaeeeaei iiiaioii, ia iao iiaeyae, ? aeyaeaiiy a AeOI ?a/iaei, caeaoieo iineethaaoe aeeoa?aioe?thaaiiy noiaao?iaeo iie?iioaioieo ee?oei e?noeiaiai iiceo, i?i ui na?ae/aoue aenia?eiaioe ia ii?ii?iaieo ieoao, o yeeo i?e aaaaeaii? AeOI ca?eueooaaeanue e?euee?noue naia e?ioi?aeieo aeaiaio?a a eieii?yo naeac?iee (oaae.1). Oaaeeoey 1 Neeaae ee?oeiieo eieii?e naeac?iee ieoae aeine?aeii? ? eiio?ieueii? a?oi, ye? io?eiaee noaeaoaeueio aeico -ii?ii?iaiiy Oei ee?oeiNeeaae ee?oei, %Aeine?aeEiio?ieueE?ioioeeoe40,010,0I??eioeeoe-i??eiaeanoe30,070,0Eaeeio eeoe (iaee/eiyaea?i? oa naaiaioiyaea?i?)2,010,0?io? ee?oeie28,010,0 Oaeoi? iae?ico iooeeie aoa aeyaeaiee ia o an?o c?aceao ae?ae?cao?a, oae naii, ye ? ?ioa?oa?ii, aeey i?iaeoeoe?? yeiai iaiao?aeia i?enooi?noue ?ioa?oa?ii?iaeoeoth/iai  e?aaiaeo. A cia/i?e i??? oea a?aeiineoueny ? aei aecia/aiiy a AeOI oaeoi?o aaeueioaaiiy i?a?aoe?? eaeeioeeo?a. I?e aea/aii? oeeoioiene/ieo aeanoeainoae AeOI ii a?aeiioaiith aei aeiaaiieo ee?oei i?aeiaa?iieo ieaaeaeee?a, aaeaii?ae?a oa enaiiaaiieo a?eo?ioeeo?a (AE) aoei aeyaeaii, ui ee?oeii? iiioeyoe?? e?ioiaeioi/iiai e?eueoey ia ?oeioaaeenue i?ae aieeaii AeOI, oiae? ye enaiiaaii? a?eo?ioeeoe i?aeaeaaaeenue e?ceno i?e 16-aiaeeiiiio eiioaeo? c AeOI. C oaeith ae i?eaeecii ?ioaineai?noth ?oeioaaeenue in vitro a i?enooiino? AeOI iooeeii? aeiaaii? ee?oeie-i?oai? ea?oeeiiie ai?oai? Hep-2 oa a?eo?ieaeeico ethaeeie E-562 (?en.2). ?en. 2 Oeeoioiene/ia aeoeai?noue AeOI ii a?aeiioaiith aei enaiiaaiieo yae?iai?uoth/eo a?eo?ioeeo?a oa iooeeiieo ee?oei IA?-2 oa E-562 in vitro Ia?iaea AeOI e?ioioeeo?a e?ia? iieieia?/ieo oai?eo oa i?aeoe/ii caei?iaeo aeiii??a noi?iaiaeaeoaaeanue iiyaith a noia?iaoaioao oeeoioiene/ieo oaeoi??a ii a?aeiioaiith aei iooeeiieo ee?oei, ye? ca ??aiai nai?? i?ioeiooeeiii? aeoeaiino? nooo?ai ia?aaeuoaaee aeoeai?noue aeania AeOI (?en.3). ?en.3 Ae?y noia?iaoaio?a, io?eiaieo i?ney eoeueoeaoaaiiy ee?oei e?ia? iieioai?eo ? i?aeoe/ii caei?iaeo in?a c AeOI, ia aeano?oeoe?th ee?oei IA?-2 oa L-929 a eoeueoo?? in vitro Iaeiei ?c aaaeeeaeo iaoai?ci?a i?ioeiooeeiii? ?acenoaioiino? ? ooieoe?iiaeueiee noai nenoaie i?e?iaei?o oeeoioiene/ieo ee?oei. Oiio oe?eaai aoei aeine?aeeoe aieea AeOI ia ?o aeoeai?noue, a oaeiae ia ai?no a e?ia? ?o ii?oieia?/iiai iin?y – aaeeeeo a?aioey?ieo e?ioioeeo?a (AAE). Anoaiiaeaii, ui aieea AeOI ia aeoeai?noue IOeE aoa ??ciini?yiiaaiei ? aaaaoi a /iio caeaaeaa a?ae aeo?aeiiai ??aiy ?o aeoeaiino?. I?e noaoenoe/i?e ia?iaoe? iaoa??aeo anoaiiaeaii, ui o oai?eo e?ioina?eiiith aa?oi?o aeeoaeueieo oeyo?a IOeE e?ia? aeoeaoaaeenue aai i?eai?/oaaeenue a ??ai?e i???, oi/a i?e ?ioaa?aeueiiio i?aeoiae? aaeoi? ci?i aoa ni?yiiaaiee a a?e noeioeyoe?? aeoeaiino? IOeE. O oai?eo ia ?ae ai?oai? oaeeo oetheooaoe?e o iai?yieo iiaeoethth/i? ae?? AeOI ia aeyaeaii. Anoaiiaeaii aenieo noeioethth/o aeoeai?noue AeOI ii a?aeiioaiith aei i?e?iaei?o oeeoioiene/ieo ee?oei oe??? eaoaai??? oai?eo (oaae.2). Oaaeeoey 2 Aieea AeOI ia aeoeai?noue IOeE e?ia? oai?eo ia ?ae ai?oai? IieacieeeAeo?ae ?cioiia Cr51 c i?oaiae, % Aeo?aeiee ??aaiue aeoeaiino? IOeE??aaiue aeoeaiino? IOeE i?e aeiaeaaaii? AeOII13,829,2*IE0 – 367,5 – 72n88 I?ei?oea: I – na?aaei? cia/aiiy; IE – iaae? eieeaaiue; n – e?euee?noue niinoa?aaeaiue; * - aeinoia??iinoue a?aei?iiinoae o ii??aiyii? c aeo?aeieie iieacieeaie (p < 0,05) Aiae?coth/e aeai? i?i aieea AeOI ia i?iaeoeoe?th oeeoie?i?a, ianaiia?aae OII, e?ioioeeoaie iieieia?/ieo oai?eo, i?yio oeeoioiene/io ae?th AeOI ia ?yae a?noiaaiaoe/ii a?aei?iieo iooeei in vitro oa caeaoi?noue ae?ae?caaaeueieo /eiiee?a ?c e?ioioeeo?a oiicee aeoea?coaaoe nenoaio IOeE iieieia?/ieo oai?eo, aeioe?eueii aaaaeaoe, ui i?aeiaa?ii? ieaaeaeeee i?noyoue ?yae a?ieia?/ii aeoeaieo ?a/iaei, ye? caeaoi? aeoeaii iiaeoethaaoe iaoai?cie i?ioeiooeeiii? ?acenoaioiino? i?aai?cio ethaeeie. C a?aooaaiiyi oeeo aeaieo AeOI iiaeia ?icaeyaeaoe ye ?ioiiaeoeaiee a?ii?aia?ao aeey canoinoaaiiy a iieieia?/i?e i?aeoeoe?. I?e aaaaeaii? AeOI ieoai c ea?oeeiiiith Eueth?n aoei aeyaeaii, ui a aenia?eiaioaeuei?e a?oi? oaa?ei, ye? io?eioaaee AeOI, iooeeie c`yaeeeny ia oeaeaeaiue i?ci?oa, i?ae a eiio?ieuei?e a?oi? (?en.4). Aeeiai?ea caaeaae? oaa?ei iiiii iiiii iiiii iiiii iii iiiii iiiii iiiii iiiii iiiii iii iiiii iiiii iiiii iiiii iiiii iiiii iiiii iii ii ii iiiii iiiii iiiii iiiii iiiii iiiii iiiii iiiii iiiii iiiii ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ***** ** ***** ***** ***** ***** ***** ***** ***** **** *** *** *** *** ** ** 1 2 3 4 5 6 7 8 9 10 Oeaei? I?iya iooeei a aeeiai?oe? : – – – + + + + + Aeine?ae – – + + + + + + Eiio?ieue Neiaieaie * oa 0 iicia/aii iaeio iniaeio a a?oi? ?c 5 oaa?ei. ?en.4 Aieea ae?ae?caaaeueiiai oaeoi?o ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a ethaeeie ia aeaye? oa?aeoa?enoeee iooeeiiiai i?ioeano o ieoae i?e i?euaieaii? ?i aenia?eiaioaeueii? iooeeie (ea?oeeiiie Eueth?na). Aeine?aeaeaiiy ii?oieia?/iiai neeaaeo e?ia? aeyaeei aeinoia??ia ca?eueoaiiy e?eueeino? aaeeeeo a?aioeiai?nieo e?ioioeeo?a a e?ia? ieoae, yeei aaiaeeee AeOI. Oeae oaeo i?aeoaa?aeaeo? ?ai?o io?eiai? aeai? i?i nooo?ao i?ioeiooeeiio ni?yiiaai?noue ae?? ?a/iaei, ye? i?noyoueny a AeOI. Oaeei /eiii, i?iaaaeai? aeine?aeaeaiiy ii aeae?eaiith ?c i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o ?ioiiaeoeaieo iaioeaeii-a?eeiaeo noanoaioe?e na?ae/aoue, ui, oe?eeii eiia??ii, oe? e?ioi?aei? ooai?aiiy i?noyoue oe?ieee niaeo? oa?iaio?a ? oeeoie?i?a, ye? ? aecia/athoue ni?yiiaai?noue ae?? AeOI. ?c i?eaaaeaiiai ae?aiaciio ?ioiiiiaeoethth/eo aoaeo?a /?oei aeo?ea? iiaeeea?noue i?iaaaeaiiy ei?eaoth/i? oa?ai?? ?ioiiaeao?oeeoieo noai?a, a ia?oo /a?ao o iieieia?/ieo oai?eo. Oaeo iecueei? aeoeaiino? AeOI ?c ieaaeaeee?a aeayeeo oai?eo ia o?ii?/iee oiicee?o iiaea na?ae/eoe aai i?i ao?aoo ?o ee?oeiaie ?ioiieia?/ieo ooieoe?e a ?acoeueoao? /eneaiieo caiaeaiue ? cai?uaiiy niieo/iith oeaieiith aeoeaii? ia?aio?ie, aai i?i ooieoe?iiaeueio aeieaaeo ee?oei-i?iaeooeaio?a ?ioiiaeoeaieo iieaeoe /e ?aoeaioi?ieo no?oeoo? (Berstein et. al., 1994; Murakata et. al., 1996). Iaeiei ?c aaaeeeaeo iiiaio?a, ui aeo?eathoue ?c ?acoeueoao?a aeaiiai aeine?aeaeaiiy, ? oie oaeo, ui iaa?oue a oiiaao o?ii?/iiai, ye i?aaeei, i?iaeoeoeaiiai caiaeaiiy i?aeiaa?ii? ieaaeaeeee ethaeeie i?iaeiaaeothoue caeeoaoeny aaaeeeaei ?ioiieia?/ii aeoeaiei i?aaiii. Aeeeth/aiiy eiai ?c aca?iiiia`ycaii? ia?aae? i?aai?a nenoaie ?ioi?oaoo oi/a ? ia i?ecaiaeeoue aei cia/iiai iineaaeaiiy ?ioiiiai noaoono i?aai?cio, oa ana ae, i?e iaaieo ianoaaeiao, iiaeia iaaaoeaii a?aeaeoeny ia iaoai?ciao caoenoo oa aaeaioaoe??. A ca`yceo c oeei ?ic?iaea iiaeo aoaeoeaieo iaoiae?a ae?aaiinoeee oa e?eoaaiiy o?ii?/iiai oiicee?oo i?iaeiaaeo? caeeoaoeny aeooaeueiei caaaeaiiyi no/anii? ioiea?eiaieia??. ?ic?iaeaia oaoiieia?y eaai?aoi?iiai io?eiaiiy ae?ae?caaaeueiiai oaeoi?o ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o iiaea neoaeeoe iniiaith aeey aeine?aeaeaiue ii noai?aiith e?eoaaeueieo cania?a iiaiai iieie?iiy. Aeniiaee 1. ?c ee?oei i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o iiaeia aeae?eeoe ?ioiiaeoeai? ?a/iaeie iaioeaeii-a?eeiai? i?e?iaee, ye? aeoeaii iiaeoeththoue ?aaeoe?? nenoaie ?ioi?oaoo ? iineeththoue ae?th oaeoi??a ianiaoeeo?/ii? ?acenoaioiino?. 2. Ae?ae?caaaeuei? oaeoi?e ?c i?aeiaa?iieo ieaaeaeee?a ? iaioeaeii-a?eeiaeie noanoaioe?yie c iieaeoey?iith ianith aei 25 eAe, ye? iathoue iaaio i?ioaie?oe/io aeoeai?noue, iaoiiaeaio iayai?noth iecueeiiieaeoey?ieo oa?iaio?a. 3. AeOI caeaoai ia?aiineoe niia?eueiaio a?ia?/ooeea?noue aei aioeaaia no?aioieieeo, a oaeiae nooo?ai noeioethaaoe ??aaiue aoi?eiii? aoii?aeueii? a?aeiia?ae? ia no?aioie?cei-I. 4. AeOI i?eoaiaii? aeanoeaino? o?ainoa?-oaeoi?a, oaeoi?a iae?ico iooeei, oaeoi?a aaeueioaaiiy i?a?aoe?? ee?oei, eieii??noeioethth/iai oaeoi?a, a oaeiae aeoeai?noue, ui ni?ey? ca?eueoaiith /enea O-e?ioioeeo?a, ye? aeni?anothoue ND-2 ?aoeaioi?e. 5. A oiiaao aenia?eiaioo aeyaeaia caeaoi?noue AeOI ci?ithaaoe iai?yi aeeoa?aioe?thaaiiy noiaao?iaeo iie?iioaioieo ee?oei e?noeiaiai iiceo ii?ii?iaieo ieoae a a?e ee?oei e?ioi?aeiiai ?yaeo. O oaa?ei-iooeeiiiin??a aaaaeaiiy AeOI i?ecaiaeeei aei ca?eueoaiiy ai?noo a e?ia? aaeeeeo a?aioey?ieo e?ioioeeo?a. 6. AeOI, ca oiia oanooaaiiy in vitro, ia ia? oeeoioiene/ii? ae?? ii a?aeiioaiith aei aeiaaiieo a?eo?ioeeo?a e?ia? ? e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a, iaeiae caeaoai aeeeeeaoe e?cen iooeeiieo ee?oei oeio Hep-2 i K-562, a oaeiae enaiiaaiieo a?eo?ioeeo?a, ye? i?noyoue yae?i. Ia?iaea ae?ae?caaaeueiei oaeoi?ii e?ioioeeo?a e?ia? ethaeeie noi?iaiaeaeo?oueny iiyaith a noia?iaoaioao oeeoioiene/ieo noanoaioe?e, ye? ca nai?th e?oe/iith caeaoi?noth ii a?aeiioaiith aei iooeeiieo ee?oei ia?aaeuothoue nai AeOI. 7. AeOI noeioeth? i?ioeiooeeiio ?acenoaioi?noue, i?aeaeuo? aeoeai?noue i?e?iaei?o e?ea?ieo ee?oei e?ia? o oai?eo ?c ceiye?nieie iooeeiaie aa?oi?o aeeoaeueieo oeyo?a, cao?eio? ?icaeoie iooeeiiiai i?ioeano o ieoae c ia?auaieaiith ea?oeeiiiith Eueth?n. I?aeoe/i? ?aeiiaiaeaoe?? 1. A?aoiaoth/e, ui i?aeiaa?ii? ieaaeaeeee i?noyoue ?ioiiaeoeai? noanoaioe??, caeaoi? aeoea?coaaoe iaoai?cie aioeaaeoa??aeueii?, aioea??onii?, i?ioeiooeeiii? ?acenoaioiino? ? aeioe?eueiei aeio?eioaaoeny a?eueo aeeoa?aioe?eiaaiiai i?aeoiaeo aei iia?aoeaiiai e?eoaaiiy o?ii?/iiai oiicee?oo. 2. ?aeiiaiaeiaaii canoinoaaiiy ae?ae?caaaeueieo oaeoi??a, i?ney a?aeiia?aeii? ia?iaee oa oanooaaiiy, aeey e?eoaaiiy o?ii?/ieo ?ioaeoe?e oa ?ioiiaeao?oeeoieo noai?a, a oiio /ene? coiiaeaieo iooeeiiei i?ioeanii. NIENIE I?AOeUe, IIOAE?EIAAIEO CA OAIITH AeENA?OAOe?? 1. Aieea ae?ae?caoo c ee?oei i?aeiaa?iieo ieaaeaeee?a o oai?eo ia o?ii?/iee oiicee?o ia aoi?eiio ?ioiio a?aeiia?aeue aei no?aioie?-ceio-I // AEo?i.ooiuo, iiniauo e ai?eiauo aieaciae. – 1997. - ? 2 – N.46-49 / Ni?aaao.: Iaeueieeia I.O., Caaieioiee Ae.?., Aa?ai??iei E.I. 2.Aeeyiea aeeaeecaaaeueiuo oaeoi?ia ec eeioioeeoia iaaiuo ieiaeaeei o aieueiuo o?iie/aneei oiiceeeeoii ia ?acaeoea iiooieaaiai i?ioeanna a yenia?eiaioa // AEo?i.ooiuo, iiniauo e ai?eiauo aieaciae. – 1997. - ? 3. – N.84-85 / Ni?aaao.: Iaeueieeia I.O., Caaieioiue Ae.E., Aa?aiaaiei E.I., Oei/aiei N.A. 3. Eiioiiaeoeaiua iaioeaeu a iaaiuo ieiaeaeeiao aieueiuo o?iie/aneei oiiceeeeoii e eo ?ieue a caueoiuo ?aaeoeeyo // AEo?i.ooiuo, iiniauo e ai?eiauo aieaciae. – 1997. - ? 5. – N.88-93. / Ni?aaao.: Iaeueieeia I.O. 4. Iieo/aiea e eiioiiiiaeoee?othuea naienoaa ieceiiieaeoey?iiai aeeaeecaoa ec eeaoie iaaiuo ieiaeaeei aieueiuo o?iie/aneei oiiceeeeoii // Oac. aeiee. VI cuacaea ioiea?eiaieiaia Aaea?one. – Aeoaane. - 1996. - N.45 / Ni?aaao.: Iaeueieeia I.O., Aa?aiaaiei E.I., Caaieioiue Ae.E., Eecei A.E., Naiao? I.A. 5. A?ieia?/ia aeoeai?noue ae?ae?caaaeueii? o?aeoe?? oiiceeioeeo?a ethaeeie // Oeceie. aeo?iae. – 1996. – O.42. - ?3-4. – N.50-51 / Ni?aaao.: Iaeueieeia I.O., E?c?i I.E., Naiao? I.Ae., Oei/aiei N.A., Eaeeiianueea E.I., Ca?oeue O.A 6. Iaea?aeaiiy oa aeine?aeaeaiiy aieeao ae?ae?caaaeueii? o?aeoe?? c e?ioioeeo?a ieaaeaeee?a ethaeeie ia iaoai?cie i?ioeiooeeiii? ?acenoaioiino? a oiiaao in vitro // No/ani? i?iaeaie ioiea?eiaieia?? – Ee?a, 1998. – N.77-83 / Ni?aaao.: Iaeueieeia I.O., Aa?ai??iei E.I., Naiao? I.Ae., Oei/aiei N.A., Aieiai?iaeueei I.I., Ea?iaiei A.O., Ca?oeue O.A., E?eaioaonueea E.Ae., Oei/aiei I.Ae. Eae?ca N.I. – ?ioii-a?ieia?/ia ae?y ae?ae?caaaeueieo /eiiee?a ?c e?ioioeeo?a ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o. – ?oeiien. Aeena?oaoe?y ia caeiaoooy a/aiiai nooiaiy eaiaeeaeaoa iaaee/ieo iaoe ca niaoe?aeuei?noth 14.01.19 – ioiea?eiaieia?y. – Ee?anueeee iaoeiai-aeine?aeiee ?inoeooo ioiea?eiaieia?? ?i. i?io. I.N.Eieii?e/aiea, Ee?a, 1998. ?c e?ioioeeo?a i?aeiaa?iieo ieaaeaeee?a oai?eo ia o?ii?/iee oiicee?o iaoiaeaie i?aia?aoeaii? a?io?i?? aeae?eaii ae?ae?caaaeueiee oaeoi? (AeO) II <25 eAe ?c oe?ieei niaeo?ii ?ioiiiiaeoethth/eo aieea?a. Inoaii?e yaey? niaith noi?o iie?iaioeae?a ?c caeeoeiaith e?euee?noth iecueeiiieaeoey?ieo a?ee?a, i?ioaie?oe/ieo oa?iaio?a oa aeeth/a? aioei?ioaaci? aeoeaiino?. AeO caeaoai ia?aiineoe a?ia?/ooeea?noue niia?eueiaii? ae?? aei no?aioie?ceio-I, iineethaaoe aoii?aeueio ?ioiio a?aeiia?aeue aei aeacaiiai aioeaaio oa ca?eueooaaoe in vitro e?euee?noue O-e?ioioeeo?a, ui aeni?anothoue ND-2 ?aoeaioi?e. Aeo?aeiee ae?ae?cao caeaoiee aeoeaoaaoe iaoai?cie i?ioeiooeeiii? ?acenoaioiino?: aaeueioaaoe ?icaeoie iooeeiiiai i?ioeano o ieoae c i?euaieeiith ea?oeeiiiith Eueth?n, aeoeaoaaoe i?e?iaei? e?ea?i? ee?oeie oai?eo ia ?ae ai?oai?, a oaeiae aeeeeeaoe e?cen ee?oei iooeeiieo e?i?e Hep-2, K-562, aea ia aeiaaiieo e?ioioeeo?a oiicee. ?ic?iaeaia oaoiieia?y eaai?aoi?iiai io?eiaiiy AeO iiaea neoaeeoe iniiaith aeey noai?aiiy e?eoaaeueiiai i?aia?aoo. Eeth/ia? neiaa: i?aeiaa?ii? ieaaeaeeee, o?ii?/iee oiicee?o, ae?ae?caaaeueiee oaeoi?, i?ioeiooeeiia ?acenoaioi?noue, oeeoie?ie. Eaeeca N.A. – Eiioii-aeieiae/aneia aeaenoaea aeeaeecaaaeueiuo oaeoi?ia ec eeioioeeoia iaaiuo ieiaeaeei aieueiuo o?iie/aneei oiiceeeeoii. – ?oeiienue. Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa iaaeeoeeineeo iaoe ii niaoeeaeueiinoe 14.01.19 – ioiea?eiaieiaey. – Eeaaneee iao/ii-enneaaeiaaoaeueneee einoeooo ioiea?eiaieiaee ei. i?io. I. N. Eieiiee/aiei, IC Oe?aeiu, Eeaa, 1998. Ec eeioioeeoia iaaiuo ieiaeaeei aieueiuo o?iie/aneei oiiceeeeoii iaoiaeaie i?aia?aoeaiie aeioeiee auaeaeai aeeaeecaaaeueiue oaeoi? n iieaeoey?iie iannie< 25 eAe n oe?ieei niaeo?ii eiioiiiiaeoee?othueo aeeyiee. Iineaaeiee i?aaenoaaeyao niaie nianue iieeiaioeaeia, n inoaoi/iui eiee/anoaii ieceiiieaeoey?iuo aaeeia, i?ioaieeoe/aneeo oa?iaioia, e aeeth/aao aioei?ioaaciua aeoeaiinoe. AeO niiniaai ia?aiineoue aeia?/oanoaeoaeueiinoue caiaaeeaiiiai oeia e no?aioieeceio-I, oneeeaaoue aoii?aeueiue eiioiiue ioaao e yoiio aioeaaio, a oaeaea oaaee/eaaoue in vitro eiee/anoai O-eeioioeeoia, yeni?anne?othueo ND-2 ?aoeaioi?u. Aeeaeecao niiniaai aeoeae?iaaoue iaoaieciu i?ioeaiiiooieaaie ?acenoaioiinoe: oi?iiceoue ?acaeoea iiooieaaiai i?ioeanna o iuoae n ia?aaeoie ea?oeeiiiie Euethen, aeoeae?iaaoue anoanoaaiiua eeeea?iua eeaoee aieueiuo ?aeii ai?oaie, a oaeaea aucuaaoue eecen eeaoie iiooieaauo eeiee Hep-2 K-562, ii ia aeeiaaiiuo eeioioeeoia oiiceee. ?ac?aaioaiiay oaoiieiaey eaai?aoi?iiai i?iecaiaenoaa AeO iiaeao neoaeeoue iniiaie aeey nicaeaiey ea/aaiiai i?aia?aoa. Eeth/aaua neiaa: iaaiua ieiaeaeeiu, o?iie/aneee oiiceeeeo, aeeaeecaaaeueiuee oaeoi?, i?ioeaiiiooieaaay ?acenoaioiinoue, oeeoieeiu. Lakiza C.A. – Immunobiological effect of dialysable factor (DF) from lymphocytes of tonsills of the patients with chronic tonsillitis. – Manuscript. The dissertation for candidate of medical science competition in specialty 14.00.19 – otorhinolaryngology. Kiev Scientific and Research Institute of Otolaryngology named after Professor A.I.Kolomijtchenko, Kiev, 1998 Dialyzable factor with molecular masse 25 kD with wide spectrum of immunomodulating effects was extracted from the lymphocytes of the palatines tonsils of the patients with chronicle tonsillitis with the preparative biochemical methods. The last one is mixture of polipeptidies with a residual quantity of law molecular proteins, proteolitic enzyme and includes antiproteasian activities. DF is able to transfer of delayed type hypersensitivity to streptolizine-O, to intensify humoral immune answer this antigens, and also in vitro increases a number of T-lymphocytes that expressing CD2 receptors. Dyalysate is able to activate the mechanisms of antitumours resistance; to impede the development of tumour process in the mice with Lewie`s carcinomas, to activate natural killers cells of the patients with a cancer of larynx and also to cause lysis of the cells of Hep-2, K 562 tumour lines but not of the allogene tonsils lymphocytes. Elaborated technology of laboratory DF production can be a basis for the creation of medical preparation. Key words: palatines tonsils, chronic tonsillitis, dializable factor, antitumour resistance, cytokines.

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