Iaoe?iiaeueia aeaaeai?y iaoe Oe?a?ie

Iinoeooo iieaeoey?ii? aiieiai? oa aaiaoeee IAIO

Ia i?aaao ?oeiieno

Eiaeooiaa Iaoaeiy Aaeoa?aeiaia

OAeE 575.11.113:854.78

Aeineiaeaeaiiy iieaeoey?ii-aaiaoe/iiai

iieiii?oicio aaiiio eoeo?oaece

iaoiaeii iieiia?acii? eaioethaiaii ?aaeoeii

03.00.26-iieaeoey?ia aaiaoeea

Aaoi?aoa?ao aeena?oaoe??

ia caeiaoooy iaoeiaiai nooiaith

eaiaeeaeaoa aiieiai/ieo iaoe

Ee?a — 1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia o a?aeae?e? aaiii? ?iaeaia??? Naeaeoe?eii-aaiaoe/iiai
?inoeoooo OAAI (i.Iaeana)

Iaoeiaee ea??aiee:

aeieoi? a?ieia?/ieo iaoe, i?ioani?,

/eai-ei?. OAAI

Neaieai TH??e Ieoaeeiae/,

Naeaeoe?eii-aaiaoe/iee ?inoeooo OAAI,
caa.a?aeae?eii

aaiii? ?iaeaia???

Io?oe?ei? iiiiaioe:

aeieoi? a?ieia?/ieo iaoe, i?ioani?

Iaethoa Noai?neaa Noai?neaaiae/

caa?aeoaa/ a?aeae?eii iieaeoey?ii? aaiaoeee

?inoeoooo iieaeoey?ii? a?ieia?? ? aaiaoeee IAI
Oe?a?ie

eaiaeeaeao a?ieia?/ieo iaoe

Eiia?i?oeueeee ?ai? Eeeiaio?eiae/

noa?oee iaoeiaee ni?a?ia?oiee

?inoeoooo ee?oeiii? a?ieia?? ?

?iaeaia??? IAI Oe?a?ie

I?ia?aeia onoaiiaa:

?inoeooo o?c?ieia?? ?ineei ? aaiaoeee IAI Oe?a?ie

Caoeno aeena?oaoe?? a?aeaoaeaoueny 20 ea?oiy 1999 ?. i 10 aiae. Ia
can?ae?ii? Niaoe?ae?ciaaii? A/aii? ?aaee Ae 26.237.01 a ?inoeooo?
iieaeoey?ii? a?ieia?? ? aaiaoeee IAI Oe?a?ie ca aae?anith: 252143,
i.Ee?a-143, aoe. Caaieioiiai, 150

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? ?inoeoooo
Iieaeoey?ii? a?ieia?? ? aaiaoeee IAI Oe?a?ie

Aaoi?aoa?ao ?ic?neaii 19 aa?aciy 1999 ?.

A/aiee nae?aoa? Niaoe?ae?ciaaii? A/aii? ?aaee

eaiaeeaeao a?ieia?/ieo iaoe
E.E.Eoeao

Aeooaeueiinoue oaie. Iaeaioeoieaoeiy aaiioeiia ?ineei —
ni?oia, eiiie, aia?eaeia oa aecia/aiiy io aaiaoe/ieo
acaaiiaiaeiinei a iaeiiath c iniia naeaeoeii ?ineei. A inoaiii ?iee aeey
oa?aeoa?enoeee aaiaoe/iiai iaoa?iaeo aeei?enoiaothoue iaoiaee, ui
caniiaaii ia i?ioiethaaiii AeIE, cie?aia, oeyoii aiaeico
i?iaeoeoia iieiia?aciii eaioethaiaii ?aaeoeii (IE?). Iaeaieueoa
iioe?aiiy a aeineiaeaeaiii iieaeoey?ii-aaiaoe/iiai iieiii?oecio
ciaeoee aeaa iaoiaee AeIE-i?ioiethaaiiy. O ia?oiio aeei?enoiaothoue
ei?ioei i?aeia?e aiaeiiii iineiaeiaiinoi, aea ia?aii ia aeiaieueiie
iniiai — aeiaieueii i?aeiiaaia IE?. Ae?oaee iiaeoiae caniiaaiee ia
canoinoaaiii aeaio niaoeeoi/ieo i?aeia?ia aeey aiieioieaoeii
eiie?aoiiai ?aaiiio AeIE, yeee iinoeoue iie?inaoaeioii iiaoi?e —
SSR-IE?. I?aeoe/ii aeiiae aei aeai?o oaoiiee AeIE-i?ioiethaaiiy
neeaaeathoueny c aiaeiinii iaaaeeeeo aeo?ao eiooia oa /ano, oaoii/iii
i?inoioe, iaaeieiinoi, aiaeoai?thaaiinoi.

Aeei?enoaiiy ?iciiiaiioieo iaoiaeia AeIE-i?ioiethaaiiy aeey
iaeaioeoieaoeii oa aeeoa?aioeiaoeii aaiioeiia oeiiieo
nieueneiainiiaea?nueeeo eoeueoo?, cie?aia eoeo?oaece, ?aeiiaiaeiaaii
Iiaeia?iaeiith Niieeith caoenoo iiaeo ni?oia ?ineei (UPOV — Union
for the Protection of New Varieties of Plants). Noai?aiiy aace aeaieo
AeIE-i?ioiethaaiiy eiiie oa aia?eaeia, yei oe?iei ?aeiiiaaii o
Iiaaeaiiiio ?aaiiii Oe?aiie, a aeooaeueiei oa aeioeieueiei i
aeeiioaoueny a ?aieao caaaeaiiy «Ii?aoethaaoe aaiii-iiaeaia?ii
oaoiieiaii aeiaaiinoeee oa aeaeieeoe ia?ea?e aaaeeeaioeo naeaeoeieieo
iciae nieueneiainiiaea?nueeeo eoeueoo?» i?ia?aie IOI OAAI «Oai?aoe/ii
iniiae naeaeoeii oa ianiiieoeoaa nieueneiainiiaea?nueeeo eoeueoo?»
(03.04-IA/322-97).

Iaoa oa caaaeaiiy aeineiaeaeaiiy. Iaoa ?iaioe iieyaaa a aiaeici
iieaeoey?ii-aaiaoe/iiai iieiii?oicio aaiiio eoeo?oaece ca aeiiiiiaith
aeaio oaoiie AeIE-i?ioiethaaiiy-aeiaieueii i?aeiiaaiii IE? (AeI-IE?) oa
SSR-IE?. Aeey aeinyaiaiiy iinoaaeaiii iaoe a aeena?oaoeieiie ?iaioi
ae?iooaaee ianooiii caaaeaiiy: 1. Aiaeic oa?aeoa?o oniaaeeoaaiiy
aiieieiiia; 2. Aecia/aiiy aia?eaeiinoi i?inoeo aia?eaeia; 3.
Ii?iaiyeueia aeineiaeaeaiiy aioo?ioiueiaeaeiaiai iieaeoey?ii-aaiaoe/iiai
iieiii?oicio eoeo?oaece ?icieie iaoiaeaie; 4. Aiaeic ei?aeyoeie iiae
aiieieiiaie oa aeayeeie aa?i-iiii/ii aaaeeeaeie iciaeaie.

Iaoeiaa iiaecia oa i?aeoe/ia cia/aiiy io?eiaieo ?acoeueoaoia. Iaoeiaa
iiaecia ?acoeueoaoia neeaaeaaoueny c ?ic?iaee oiia aeiai?o i?aeia?ia,
ui aeicaieythoue aeeoa?aioeithaaoe aaiioeie eoeo?oaece, ii?iaiyeueiiai
aiaeico ?icieo oeiia ia?ea?ia — aiioiii/ieo oa iieaeoey?ieo,
yei io?eiaii ?icieie iaoiaeaie AeIE-i?ioiethaaiiy, oa a
iaa?oiooaaiii aeai?o ia?ea?a aeey eiie?aoieo caaea/ aeineiaeaeaiue.

I?aeoe/ia cia/aiiy iieyaaa a iioeiicaoeii oiia IE?, cie?aia,
oaiia?aoo?iiai ?aaeeio oa neeaaeo ?aaeoeieiii noiioi. Cai?iiiiiaaia
oano-iaiaeue c oanoe SSR-eieonia aeey aecia/aiiy aia?eaeiinoi
i?inoeo aia?eaeia eoeo?oaece. I?iaaaeaia iaeaioeoieaoeiy eieonia,
yei aiaeiiaiaeathoue ca aeayei eieueeinii iciaee.

Iniaenoee aianie caeiaoaa/a. Iniaenoee aianie caeiaoaa/a noaiiaeoue:
iioeiicaoeiy oiia i?iaaaeaiiy IE?, cie?aia, neeaaeo ?aaeoeieiii
noiioi; aiaeic aioo?ioiueiaeaeiaiai iieiii?oicio Zea mays L. ?icieie
iaoiaeaie AeIE-i?ioiethaaiiy; ii?iaiyeueiee aiaeic aeene?eiiiaoeieiii
neee aiioiii/ieo oa iieaeoey?ieo ia?ea?ia; aea/aiiy oa?aeoa?o
oniaaeeoaaiiy aiieieiiia, yei io?eiaii ?icieie IE?-iaoiaeaie, o
F1-aia?eaeao eoeo?oaece; aecia/aiiy ei?aeyoeie iiae aiieieiiaie oa
aeayeeie aa?iiiii/ii aaaeeeaeie iciaeaie eoeo?oaece.

Ai?iaaoeiy ?acoeueoaoia aeena?oaoeii. ?acoeueoaoe aeineiaeaeaiue, yei
aeeth/aii a aeena?oaoeith, aeiiiaiaeaeenue ia VI Iiaeia?iaeiie
eiioa?aioeii «Frontiers in biochemistry and molecular biology. The
legacy of Andrey Belozersky» (Iineaa, 1995 ?.); Iiaeia?iaeiie
iaoeiai-i?aeoe/iie eiioa?aioeii «Ianeiaeee iaoeiaeo iiooeia iieiaeeo
a/aieo-aa?a?ieeia a oiiaao ?aoi?ioaaiiy AIE» (*aaaie, 1996 ?.);
Iiaeia?iaeiie eiioa?aioeii «Iieaeoey?ii-aaiaoe/aneea ia?ea?u ?anoaiee»
(sseoa, 1996 ?.); Iiaeia?iaeiie eiioa?aioeii «Iieaeoey?iay aaiaoeea
e aeioaoiieiaey» (Iiinuee, 1998 ?.).

Ioaeieaoeii. ?acoeueoaoe aeena?oaoeii iioaeieiaaii a o?ueio noaooyo,
oanoe oacao, iaoeiai-iaoiaee/iiio iiniaieeo.

No?oeoo?a aeena?oaoeii. Aeena?oaoeiy neeaaea3/4oueny ic anooio, oanoe
?icaeieia — iaeyaeo eioa?aoo?e, iaoa?iaeia oa iaoiaeia, ?acoeueoaoia oa
iaaiai?aiiy (o /ioe?ueio ?icaeieao); aeniiaeia.

?iaioa aeeeaaeaia ia 105 noi?iieao i iinoeoue 14 iaethieia oa 11
oaaeeoeue. Nienie aeei?enoiaaiii eioa?aoo?e aeeth/a3/4 119 aeaea?ae.

IAOA?IAEE OA IAOIAeE AeINEIAeAEAIUe

Iia?aaeii eiiii, yei aeei?enoiaoaaeenue aeey aeineiaeaeaiue
aioo?ioiueiaeaeiaiai iieiii?oecio eoeo?oaece (Zea mays L.) ca
aeiiiiiaith AeI-IE? oa aeaeo?ioi?aco caiio, ianooiii (o aeoaeeao aeacaia
iaeaaeiinoue aei aaoa?iceniii a?oie): I156Rf, HMV404, Mk159
(Mindszenpusta); F2, F7, Ma21, Ep1 (Lacaune); Mo17, Iae140, ?426,
A619, N103, Oh43 (Lancaster); CM105, W63, Wf9, A632, A634, B14,
B37, B55, B73, B76 Reid); P101, P165, P343, P480, A344 (Iodent).

Aeey aeineiaeaeaiiy aioo?ioiueiaeaeiaiai iieiii?oicio eoeo?oaece (Zea
mays L.) iaoiaeii SSR-IE? oa iiooeia anioeiaoeie iiae aiieieiiaie
oa eieonaie eieueeinieo iciae aeei?enoiaoaaee ianooiii iia?aaeii
eiiii: II150, II150ci, II151, Ii17, Ii17na, F564c, F564cn, F564-12cn,
?343i, ?101ci, I156Rfia, HMV2/75i, HMV2/75ci, HMV404ia, HMVD4-1-1,
Iae7, Iae7ia, AE26na, AE26i, AE26ci, ?165ia, Iae140ia, Iae329,
Iae322, AE11i, AE11ci, AE11, II74, IE40ci, ?354ci, AI?44ci, AI?40ci,
Iae428, Iae430, ?3295, Iae532, Iae433-1, Iae433-4, Iae437-1,
Iae437-2, Iae441-1, NA33, EE107, ?092, Iae427, ?346ci, ?502ci,
?502ia, NI7ci, EE205-2, NI586-12, Ea2087ci, W64ASc, W64AScc, RS186.

Iaoa?iaeii aeey aiaeico oniaaeeoaaiiy aiieieiiia oa aecia/aiiy
aia?eaeiinoi F1-aia?eaeia eoeo?oaece aoee aaoueeianueei oi?ie ?346 i
?502, Iae7 i Iae24, ?346 i AE26, AE26 i ?101, I156 i HMV404,
AE11 i II74, F564 i F564-12, AE26 i ?502, ?346 i EE205-2, NA33 i
EE107-1, IE40 i F2 oa aiaeiiaiaeii ii aia?eaee ?3978, Eaae?, ?ica,
Niaia, Noaaie?, Nth?i?ec, Iaeiaeiy, Noiaea?, Ne?aiue, Ieaoai, Ano?a.

Aeaeieaiiy ?ineeiiii AeIE. AeIE aeaeieyee c eiaeiiai c aeanyoueio
aoiieueiaaieo i?i?inoeia aeey aecia/aiiy oeiiainoi eiiii oa c iyoueio
i?i?inoeia o anio iioeo aeiaaeeao. Aeey oeueiai i?i?inoee ?icoe?aee
neeyiei oiaea/ai o aiaiaei?oi aei io?eiaiiy iaeii?iaeiii iane,
aeiaeaaaee 0,5 ie eicoth/iai aooa?o, aei neeaaeo yeiai aoiaeeoue 20 mM
Na EDTA, 100 mM o?en-HCl pH 8,0 (25 C), 1,4 M NaCl, 2% COAA, oa
iieoai?oaaee a oa?iinoaoi iaeio aiaeeio i?e 60oN. Aeiaeaaaee ?iaiee
ia’ai noiioi oei?ioi?io oa iciiaioaiieo (24:1) oa ?iciiooaaee ia
ai?oaeni. Aeey ?icaeieaiiy oac i?iaiaeeee 5-oaeeeiia
oeaio?eooaoaaiiy a Eppendorf 5415 i?e 14000 ia/oa. Aiaeio oaco
aiaeae?aee a iioee aiaiaei?o oa aeiaeaaaee ?iaiee ia’ai icii?iiaiieo.
AeIE inaaeaeoaaee 5-oaeeeiiei oeaio?eooaoaaiiyi i?e 14000 ia/oa.
Noia?iaoaio aeeeaaee oa aenoooaaee i?e eiiiaoiie oaiia?aoo?i 0,5
aiaeeie. Inaaeie AeIE ?ic/eiyee a 200 iee aooa?o OA ianooiiiai neeaaeo
: 10 mM o?en-HCl pH 8,0, 1 mM Na EDTA a i?enooiinoi 1 iea/ie ?IEace
A. Eiioeaio?aoeith AeIE aecia/aee ia DNA Fluorometer, model TKO 100
(Hoefer Scientific Instruments, San Francisco, USA).

Oiiae iieiia?aciii eaioethaiaii ?aaeoeii. Iieiia?acio eaioethaiao
?aaeoeith (IE?) caeienithaaee ia aiieioieaoi?i «Oa?oeee» (i.Iineaa,
?iniy) o ianooiiiio ?aaeeii: ii/aoeiaa aeaiaoo?aoeiy – 94oN, 2 oa.;
caeeth/ia aeiiaaoeiy – 72oN, 3 oa. aeey anio oeiia IE?; 30 iniiaieo
oeeeeia; aeaiaoo?aoeiy – 94oN, 1 oa; aiaeiaethaaiiy aeaeaia?ieo
naiioieo i?aeia?ia – 46oN, 1 oa.; 20-/eaiieo i?aeia?ia – 52oN, 1 oa.;
aeiiaaoeiy – 72oN, 2 oa. I?e i?iaaaeaiii SSR-IE? oiiae 30 iniiaieo
oeeeeia aoee ianooiii: 94oN, 10 nae.; 60oN, 10 nae.; 72oN, 10 nae.

?aaeoeieia noiio ia’aiii 20 iee iinoeea: 1 o aooa?, a neeaae yeiai
aoiaeeee 50 mM KCl, 20 mM o?en-HCl pH 8,4 (25iN), 4 mM MgCl2, 0,01%
oaei-20; 0,2 mM eiaeiiai dNTP; ii 0,2 mkM i?aeia?a; 20 ia AeIE; 1
iaeeieoey AeIE-iieiia?ace Taq. Ia ?aaeoeieio noiio iaiineee 20 iee
iiia?aeueiii ieii. I?e aeei?enoaiii eiaeiiai i?aeia?a caeienithaaee
iaaaoeaiee eiio?ieue.

I?iaeoeoe aiieioieaoeii AeIE (5 iee ?aaeoeieiii noiioi)
aiaeicoaaee aeaeo?ioi?acii o 2%-iio aaa?iciiio aaei i?e AeI-IE? aai a
4%-iio aaa?iciiio aaei i?e i?iaaaeaiii SSR-IE? a 1oOAA (50mM
o?en-H2BO3, 1 mM Na -EDTA pH 8,0) 5-7 aiaeei i?e 100-120 V ia aia?aoi
Hoefer Scientific Instruments (San Francisco, USA) i?e 4oN,
oa?aoaaee a?iienoei aoiaei3/4i i oioia?aooaaee a OO-naioei ia oioiieiaeo
«Iie?ao 300».

Aeaeo?ioi?aoe/ii i?ioiei aiieioieiaaii§ AeIE eiaeiiai aaiioeio
ioeiithaaee aicoaeueii e eiaeoaaee aiia?ii: iayaiinoue aai
aiaenooiinoue nioae iicia/aee «1» /e «0» aiaeiiaiaeii.

Eiii’thoa?iee aiaeic iaea?aeaieo ?acoeueoaoia. Aeey anoaiiaeaiiy
aaiaoe/ieo aeenoaioeie ca iaoiaeii Nei, Li i iiaoaeiae aeaiae?ia?ai
aca3/4iiaiaeiinei aaiioeiia ca iaoiaeii eeanoa?icaoeii «UPGMA» ia
iniiai aeaieo aeaeo?ioi?aoe/iiai ?iciiaeieo aieeia oa aiieieiiia
aeei?enoiaoaaee eiii’thoa?io i?ia?aio «TREE 4.0». Aeey aecia/aiiy
ei?aeyoeie iiae aiieieiiaie oa eieonaie eieueeinieo iciae
canoiniaoaaee eiii’thoa?io i?ia?aio «MAP_QTL». Iieacieeii ai?iaiaeiinoi
na?aaeiio cia/aiue aa?iiiii/ieo iieacieeia aoa ia?aiao?e/iee e?eoa?ie
No’thaeaioa (t-e?eoa?ie), yeee aecia/aee ye aiaeiioaiiy ?icieoei
na?aaeiio aeai?eiaeo aei io iiieeee. Ai?iaiaeiinoue ei?aeyoeie
ia/enethaaee ia 1% i 0,1% ?iaii cia/ouinoi.

?ACOEUeOAOE AeINEIAeAEAIUe

1. Aea/aiiy oa?aeoa?o oniaaeeoaaiiy aiieieiiia

I?e aeiai?i oa ioeiioei aaiaoe/iiai ia?ea?o aaeeea cia/aiiy iaa
i?iaiicoaaiiy eiai oniaaeeoaaiiy. Aeey oiai, uia aecia/eoe
iiaeeeainoue aeei?enoaiiy IE? aeey iaeaioeoieaoeii iia?aaeieo eiiie
i?inoeo aia?eaeia eoeo?oaece, i?iaaee oanooaaiiy F1-aia?eaeia oa
ioeiieee oa?aeoa? oniaaeeoaaiiy aiieieiiia. Aeineiaeaeoaaee 33 aaiioeie
eoeo?oaece: 18 aaiioeiia ca aeiiiiiaith IE? c iyoueia aeiaieueieie
i?aeia?aie i 30 — c oinoueia ia?aie SSR-i?aeia?ia. Anueiai aoei
aeineiaeaeaii 246 aiiiiiieo IE?-eieonia (82% iieiii?oieo) i
oinoue SSR-eieonia.

C 203 iieiii?oieo aiieieiiia, io?eiaieo oeyoii AeI-IE?, 3,94%
iieacaee «iaiaiaeaeianueeo iiaaaeiieo»: aeey 3,53% aiaecia/aia
aiaenooiinoue aaoueeianueeiai o?aaiaioo a AeIE-niaeo?i aia?eaea, a aeey
0,5% — iayaiinoue o AeIE-niaeo?i aia?eaea o?aaiaioo, yeee ia e
oa?aeoa?iei aeey aaoueeianueeeo niaeo?ia; iioi aiieieiie
oniaaeeiaoaaeenue aeiiiiaio-?aoeaneaiei oeyoii. I?e SSR?-aiaeici ii
oanoe eieonao «iaiaiaeaeianueeo iiaaaeiieo» aiaecia/aii a 5%
aeiaaeeia. Iaaiai?ththoueny iiaeeeai iiyniaiiy «iaiaiaeaeianueeii
iiaaaeiiee» aiieieiiia i?e AeI-IE? oa SSR-IE?.

*a?ac oa, ui aia?eaeia aaoa?iceaioa /anoioa aeyaeyeoueny i?e
SSRP-aiaeici, oeae iaoiae aeei?enoiaoaaee aeey aecia/aiiy
«aia?eaeiinoi» i?inoeo aia?eaeia eoeo?oaece. Iayaiinoue
eiaeiiiiaioiii aaoa?iceaioe anoaiiaeaii o 80% aeiaaeeia ii
iaeiiio-i’yoe aaaaoiaeaeueiei aenieiaa?iaaieueiei SSR-eieonai. Aeey
eiaeiiai SSR-eieona iiae?aoiaaia eiiai?iinoue aeeeth/aiiy ?A
(probability of exclusion) iaaaoueeianueeeo /ieiai/eo oi?i oeyoii
ii?iaiyiiy c ia?aie iaoa?einueea oi?ia/iioiiie aiaeiieo aa-

iioeiia o iiioeyoeii, a oaeiae niieueia eiiai?iinoue aeeeth/aiiy
(combined probability of exclusion). Aeey oinoueio aeineiaeaeaieo iaie
SSR-eieonia cia/aiiy ?A neeaaeaee: aeey adh2n — 0,57; ppdka2 —
0,18; phi064 — 0,65; phi085 — 0,18; phi016 — 0,41; phi061 — 0,16.
O ca’yceo c oei, ui aeineiaeaeoaaii eieono ia c/aieaii, N?A neeaea
0,95. Oea cia/aiiy iieaco3/4, ui iaaaoueeianueea /ieiai/a oi?ia
iiaea aooe iai?aaeeueii iaeaioeoieiaaia ye aaoueeianueea eeoa o 5%
ii?iaiyiue.

Neooaoeie, eiee ii iaeiiio eieono io?eiaii eiaeiiiioaaiiy, a ii iioiio
— «iaiaiaeaeianueea iiaaaeiiea», ia niinoa?iaaeinue. Iaiiaeeeainoue
iaeaioeoieoaaoe aeaa aia?eaee iia’ycaia c iiiiii?oiinoth
aaoueeianueeeo eiiie i aiaeiiaiaeiiai aia?eaea a iaeiiio aeiaaeeo, oa,
iiaeeeai, canii/aiiyi iaoa?einueeii aaoueeianueeii eiiii a iioiio
aeiaaeeo. Oiio i?e oanooaaiii aia?eaeiinoi aeioeieueii i?iaiaeeoe
iiia?aaeiie aiaeic oeiiainoi eiiie oa /enoioe aia?eaeiiai ianiiiy.
Aeey oeueiai aeinoaoiuei i?iaanoe aiieioieaoeith AeIE ic 10
iiaeeaiaeooiia c aeei?enoaiiyi iaeiiei ia?e i?aeia?ia, oa i?e
iayaiinoi iaei§ eieueeinoi iaoeiiaeo niaeo?ia (aei 10%) o iiaeaeueoeo
aiaeicao aeei?enoiaoaaoe AeIE c oeiiaei niaeo?ii. sseui aiaenioie
iaoeiiaeo niaeo?ia AeIE aoaea aenieei, a oaeiae yeui eiiii aoaeooue
iieacoaaoe aaeeeee aiaenioie aaoa?iceaio, i?aaiii?ii c?iaeoe aeniiaie
i?i canii/aiiy eiiii. Aecia/aiiy aea?eaeiinoi a oaeiio ?aci
iaaeioeieueii.

2. Aeineiaeaeaiiy aioo?ioiueiaeaeiaiai iieiii?oicio eoeo?oaece ca
aeiiiiiaith aiioiii/ieo i iieaeoey?ieo ia?ea?ia

Ioeiiea aaiaoe/iii ?iciiiaiioiinoi aeaea?ae ca?iaeeiaii ieacie e
iaeiei c iniiaieo caaaeaiue naeaeoeieieo i?ia?ai iieiioaiiy eoeo?oaece.

O iiaeaiie ?iaioi aeineiaeaeaiee ?yae iia?aaeieo eiiie eoeo?oaece c
aiaeiiith iaeaaeiinoth aei aaoa?icenieo a?oi ca aeiiiiiaith AeI-IE?
c iaoith ?iciiaeieo eiiie ca nooiaiai io aaiaoe/iii aiaeaeaeaiinoi
oa ii?iaiyiiy aeaieo c ?acoeueoaoaie, io?eiaieie aiaeicii
aeaeo?ioi?aoe/ieo niaeo?ia caiio.

AeIE-i?ioiei eiiie io?eiaii aiieioieaoei3/4th c aeanyoueia
aeiaieueieie i?aeia?aie. Iiaeia?aii i?aeia?e aeicaieeee aeine?eiiioaaoe
oni eiiii, oiaoi niaeo?e AeIE aoee oiieaeueii aeey eiaeiii eiiii i iaee
aiae 10 aei 17 eiiiiiaioia. I?iaiaeiciaaii 187 aiieieiiia, c eio?eo
76% aoee iieiii?oii.

Ia aeaiae?ia?aii ca’yceia iiae eiiiyie aeaeieeeenue aeaa iniiaieo
eeanoa?a. Ia?oee eeanoa? aeeth/aa aeai eiiii aaoa?iceniii a?oie
Lancaster: Oh43 e A619, iioiaeaeaiiy yeii iia’ycaii c Oh43
([A171xOh43]xOh43). Ae?oaee eeanoa? neeaaeaany c eieueeio
noaeeanoa?ia. O ia?oiio noaeeanoa?i ca?oioaaeeny eiiii aaoa?iceniii
a?oie Lacaune o?aioeocueeii naeaeoeii (F2, F7, Ma21, Ep1) i eiiii
aaoa?iceniii a?oie Iodent (P430, P343, P165, P101, A344, Iae430). A
iaeiiio noaeeanoa?i ?iciiuaii eiiii A344 i AI?44, yei iathoue
i?aeoe/ii iaeei aaiioei, aea yei ?ai?iaeoeoaaeenue aaaaoi ?ieia o ?icieo
aeieiai-aaia?aoe/ieo ciiao. (Eiiiy AI?44 e aiaeiaii aia?eeainueeii
eiiii A344.) Eiiii aaoa?iceniii a?oie Reid (A55, A73, ?346; B14 i
nii?iaeiaii ie A632, A634 i NI105) noai?eee ae?oaee noaeeanoa?. O
oeae noaeeanoa? oaeiae aieoee aeai eiiii aaoa?iceniii a?oie Lancaster
Mo17 e C103, yea e iaeiieth c aaoueeianueeeo oi?i Ii17. Aea aiie ia
aoee iiia?ii iia’ycaii.

O?aoie noaeeanoa? aeeth/aa o?e eiiii aaoa?iceniii a?oie
Mindszenpusta (nano?einueei eiiii HMV404, I156Rf, Ie159).

Iiiiiaeueii aaiaoe/ii aeenoaioeii aoee io?eiaii iiae aaoa?icenieie
a?oiaie Lacaune i Iodent (na?aaeie 0,286), a iaeniiaeueii —
iiae aaoa?icenieie a?oiaie Lancaster i Mindszenpusta (na?aaeie
0,428) (oaae.1).

Iaeiaioa eieeaaiiy cia/aiiue aaiaoe/ieo aeenoaioeie aoei a iaaeao
aaoa?iceniii a?oie Lacaune, a iaeaieueoa — iiae eiiiyie aaoa?iceniii
a?oie Lancaster. O oeieiio, a?oioaaiiy eiiie ca aeaieie
iieiii?oicio aeiaaeei aiieioieiaaieo o?aaiaioia AeIE

caiaeinue c i/ieoaaiith ye ii iaaeia?i, oae i ii aaoa?iceniie
iaeaaeiinoi.

Aeaeo?ioi?aoe/ii niaeo?e caiio aieueoinoi aeineiaeaeaieo eiiie
eoeo?oaece aoee niaoeeoi/ieie i i?aaenoaaeaii 7-13 eiiiiiaioaie.
Aeayei aeecueeinii?iaeiaii eiiii ia ?ic?iciyeenue ca
aeaeo?ioi?aoe/ieie niaeo?aie caiio. Ic 30 eiiie, yei aeeth/aii

Oaaeeoey 1

Cia/aiiy aaiaoe/ieo aeenoaioeie iiae i a iaaeao aaoa?icenieo a?oi

Aaoa?iceniay a?oiia Lacaune Lancaster Mindszen-pusta Reid Iodent

Lacaune 0,124-0,156 0,329-0,445 0,349 0,312- 0,329 0,259-

0,312

Lancaster

0,329-0,445 0,101-0,410 0,410-0,445 0,174-0,445 0,329-0,445

Minszenpusta 0,349 0,410-0,445 0,171-0,300 0,349 0,349

Reid 0,312-0,329 0,174-0,445 0,349 0,073-0,262 0,312-0,329

Iodent 0,259-0,312 0,329-0,445 0,349 0,312-0,329 0,103-0,312

aei aiaeico, oiieaeueii niaeo?e caiio aoee o 21. Ia aeaiae?ia?aii
?iciiaeieo eiiie ca aeaieie aeaeo?ioi?aco caiio ia niinoa?iaaeinue
caeiiiii?iinoae ca aaoa?iceniith iaeaaeiinoth oa iioiaeaeaiiyi.

Aeaiae?ia?aie ?iciiaeieo eiiie eoeo?oaece, yei iiaoaeiaaii ca aeaieie
aiaeico iieiii?oicio AeIE i caianieo aieeia, ?iciyoueny a cia/iie ii?i.

Aiaeic i?iaeoeoia aiieioieaoei§ AeIE aeicaiey3/4 io?eiaoe aieueo
ia’eeoeaio, iiae i?e ?icaeyaei niaeo?ia aieeia, ea?oeio aaiaoe/ieo
acaeiiaiaeiinei na?aae eiiie eoeo?oaece, ui iia’ycaii c aieueo iiaiith a
ia?oiio aeiaaeeo iioi?iaoei3/4th i?i iiieeainoue aaiaoe/iiai iaoa?iaeo.

Iiaeiaia niooaoeiy iiaea aooe iia’ycaia c oieth ianoaaeiith, ui ciia,
yea eiaeoe caiie, ioiiethe iacia/io /anoeio aaiiio, o oie /an ye
aiieieiie e ?acoeueoaoii aiieioieaoeii ?icieo ?aaiiiia aaiiio,
aeeth/ath/e no?oeoo?ii oa iano?oeoo?ii ciie. Caiie aeathoue
iioi?iaoeith i?i iiieeainoue o /ioe?ueio eieonao, a ca aeiiiiiaith
AeIE-i?ioiethaaiiy aea/aii aieueo 200 aiiiiiieo eieonia.

Aeene?eiiiaoeieia neea AeIEiaeo ia?ea?ia ca ?iaiai
iieiii?oicio cia/ii aeua, iiae aiioiii/ieo (aieeiaeo) ia?ea?ia. Iaeiieth
c i?e/ei oeueiai e aieueoee ioaao aaiiio AeIEiaeie ia?ea?aie. Aiaeic
aeaeo?ioi?aoe/ieo niaeo?ia caiio ia aeicaieea oiieaeueii
aeeoa?aioeithaaoe ani aeineiaeaeaii eiiii, o oie /an ye aiaeic
AeIE-i?ioieia i?eaia aei iaeaioeoieaoeii anio eiiie i io
eeanoa?icaoeii, yea niiaiaaeae (e?ii aeayeeo niooaoeie) c i/ieoaaiith ca
aeaieie iaaeia?i i iaeaaeiinoi aei aaoa?icenieo a?oi.

3. Aea/aiiy iieaeoey?ii-aaiaoe/iiai iieiii?oecio eoeo?oaece iaoiaeaie
SSR- e AeI-IE?

Aeey aeeoa?aioeiaoeii oa iaeaioeoieaoeii eiiie aeei?enoiaoaaee
SSR?-aiaeic oanoe eieonia. Eieueeinoue aeaeia ia eieon aa?ithaaea aiae
aeaio aei i’yoe. Aeey eiaeiiai eieono iiae?aoiaoaaee /anoioe aeaeia
oa iiaeaene iieiii?oiinoi (Polymorphic Index Content — PIC) aeey
ioeiiee aeene?eiiiaoeieiii neee eieona (oaae.2). Na?aaeie cia/aiiy PIC
neeaaeaei 0,57.

Cia/aiiy aaiaoe/ieo aeenoaioeie ca aeaieie SSRP-aiaeeco aoee aiae 0 aei
0,62. Ia?iciiciaieie ca aeineiaeaeaieie eieonaie aeyaeeenue
aeecueeinii?iaeiaii eiiii (i?e niieueiinoi aaiiio aieueo 95%), yei
?icieeenue nenoaiith Oe*N-Rf (oeeoiieaciaoe/ia /ieiai/a noa?eeueiinoue i
aaie Rf).

Oaaeeoey 2

Cia/aiiy /anoio aeaeia i iiaeaeno iieiii?oiinoi

aeey oanoe SSR-eieonia

SSR-eieon Adh2n Ppdka2 Phi064 Phi085 Phi015 Phi061

*enei aeeaeae:

ii eeoa?aoo?iui

aeaiiui

ii ?acoeueoaoai

iaoaai aiaeeca

5

4

4

2

7

5

3

2

7

3

3

2

Cia/aiea PIC:

ii eeoa?aoo?iui

aeaiiui

ii ?acoeueoaoai

iaoaai aiaeeca

0,70

0,48

0,82

0,78

0,75

0,50

0,84

0,55

0,71

0,40

*anoioa aeeaeae 1-0,20

2-0,41

3-0,28

4-0,11 1-0,59

2-0,41 1-0,26

2-0,20

3-0,15

4-0,28

5-0,11 1-0,45

2-0,55 1-0,45

2-0,50

3-0,05 1-0,28

2-0,72

Ca ?acoeueoaoii AeI-IE? c aeanyoueia aeiaieueieie i?aeia?aie
io?eiaii 156 aiieieiiia, c eio?eo 71% aoa iieiii?oiei. Niaeo?
aiieioieiaaiii AeIE eiaeiii eiiii iinoea aiae 10 aei 17 eiiiiiaioia.
Oni eiiii aoee oiieaeueii aeeoa?aioeieiaaii. Cia/aiiy aa-

iaoe/ieo aeenoaioeie aa?ithaaee a iaaeao 0,04-0,58.

Aiaeic ca aeiiiiiaith AeI-IE? aeyaea aieueoa iieiii?oiciia, iiae
SSR?-aiaeic. O eoeo?oaece SSR?-aiaeic ia aea3/4 oaeiai aaeeeiai
caieueoaiiy aeene?eiiiaoeieiii neee, ye o nii, ioaieoei, oiiaoo. I?ioa
SSR?-aiaeic o iaoiio aeineiaeaeaiii iiaeoaa?aeea
aeecueeinii?iaeiaiinoue oaeeo ia? eiiie, ye II150 i II150ci; ?502 i
?502ia; F564n, F564cn i F564-12cn; AE11 i AE11i; AE26i i AE26ci.
Ei?aeyoeiy iiae aeaieie, io?eiaiieie aeaiia oeiaie aiaeico,
neeaaeae 0,4.

O ?ic?iaoei oai?aoe/ieo oa i?aeoe/ieo aniaeoia naeaeoeii eoeo?oaece
iaiaoiaeii canoiniaoaaoe iaeaeaa iaoiaee — SSR- i AeI-IE?, yei
aeiiiaiththoue iaeei iaeiiai i aeicaieythoue aoaeoeaii ae?iooaaoe
iaeaeooaeueiioi i?iaeaie iie?auaiiy oeiei eoeueoo?e.

4. Iiooee anioeiaoeie aiieieiiia c eieonaie, yei

eiio?ieththoue aeayei ainiiaea?nueei aaaeeeai iciaee eoeo?oaece

I?iaiaeicoaaee ei?aeyoeii iiae aiieieiiaie, io?eiaieie oeyoii AeI-IE?
e SSR-IE?, i 21 aa?iiiii/ii aaaeeeaeie iciaeaie ca eieueeinieie
iieacieeaie 1995 i 1996 ??. Ai?iaiaeiinoue ei?aeyoeie ioeiithaaee
oieueee ia aenieiio (1% e 0,1%) ?iaii cia/ouinoi. Ca aeaieie 1995 ?.
11 eieueeinieo iciae ai?iaiaeii ei?aethaaee c 11 aiieieiiaie. O 1996
?. aeaa’youe eieueeinieo iciae ai?iaiaeii ei?aethaaee c aeanyoueia
aiieieiiaie. Nii ai?iaiaeieo ei?aeyoeie niiaiaaeaee ca aeaieie iaio
?ieia. I?iaiaeicoaaee ei?aeyoeii iiae iciaeaie. Ca aeaieie 1995 ?.
ciaeaeaii 17 ai?iaiaeieo ei?aeyoeie (13 iiceoeaieo i /ioe?e iaaaoeaieo).
Ca aeaieie 1996 ?. aeyaeee 33 ai?iaiaeii ei?aeyoeii (24 iiceoeaieo i
aeaa’youe iaaaoeaieo), aei oiai ae 13 ei?aeyoeie 1995 ?. caa?iaaeenue i
o 1996 ?. Iiae iaeiaeiaeie iciaeaie ca aeaa ?iee io?eiaia iiceoeaia
ei?aeyoeiy ca aniia iieacieeaie. Iniiaii eiiiiiaioe o?iaeaeiinoi,
oaei ye iana 1000 ca?ai, eieueeinoue ?yaeia ca?ai, aenioa ?ineei,
aenioa i?ee?iieaiiy ea/aiia, iieacaee iiceoeaio ei?aeyoeith iiae
niaith ca aeaieie iaio ?ieia. Iieacaia neeaaeiinoue iaeaioeoieaoeii
eieonia eieueeinieo iciae, i?iya yeeo caeaaeeoue aiae oiia
iaaeieeoiueiai na?aaeiaeua. Ca aeaieie aeaio ?ieia ca ?icieo iiaiaeieo
oiia aecia/aii 40% ai?iaiaeieo ei?aeyoeie iaae aiieieiiaie i
eieueeinieie iciaeaie i 76% ai?iaiaeieo ei?aeyoeie iiae naieie iciaeaie.
Io?eiaii ia?ea?e iiaeia ?aeiiaiaeoaaoe aeey aiaeico
naeaeoeieii-aaiaoe/iiai iaoa?iaeo.

AENIIAEE

Aeineiaeaeaiiy aioo?ioiueiaeaeiaiai iieaeoey?ii-aaiaoe/iiai
iieiii?oicio eoeo?oaece ?icieie iaoiaeaie AeIE-i?ioiethaaiiy aeicaieeei
c?iaeoe ianooiii aeniiaee:

1. Aeey iaeaioeoieaoeii oa aeeoa?aioeiaoeii eiiie i aia?eaeia
eoeo?oaece aeioeieueii eiiieaenia canoinoaaiiy aeaio IE?-iaoiaeia, ui
aiae?iciythoueny ca eieueeinoth aeineiaeaeaieo eieonia oa oa?aeoa?ii
oniaaeeoaaiiy.

2. Aeene?eiiiaoeieia neea ia?ea?ia, io?eiaieo oeyoii AeI-IE?,
aeua, iiae aieeiaeo oa SSR-ia?ea?ia, ui aeicaieye oiieaeueii
aeeoa?aioeithaaoe eiiii eoeo?oaece (iaaioue i?e niieueiinoi aaiiiia
aieueo 95%).

3. Eeanoa?ecaoeiy eiiie ca ?acoeueoaoaie AeI-IE?-aiaeico niiaiaaeae
c i/ieoaaiith ii iaaeia?i oa ii iaeaaeiinoi aei aaoa?icenieo a?oi.

4. SSR?-aiaeic aieueo iaaeieiee i aiaeoai?thaaiee, iiae AeI-IE?,
aeey ?ioaiiy oaeeo caaea/, ye aecia/aiiy /enoioe aia?eaeiiai ianiiiy,
oeiiainoi eiiie i aaoueeianoaa i?inoeo aia?eaeia.

5. Cai?iiiiiaaia oano-iaiaeue c oanoe SSR-eieonia aeey aecia/aiiy
«aia?eaeiinoi» i?inoeo aia?eaeia eoeo?oaece, yea iieacaea aenieo
eiiai?iinoue aeeeth/aiiy (N?A=0,95).

6. O ?acoeueoaoi aeao?i/iiai aiaeico eieueeinieo iciae eiiie aecia/aii
ai?iaiaeii ei?aeyoeii iiae aiieieiiaie oa aeayeeie aaaeeeaeie
aa?iiiii/ieie iciaeaie.

Nienie ioaeieaoeie ca oaiith aeena?oaoeii

1. Neaieai TH., Eiaeooiaa I., Anuea TH. Enneaaeiaaiea aaiaoe/aneeo
acaeiiioiioaiee o eeiee eoeo?ocu i?e iiiiue RAPD e caeiia // Oeeoieiaey
e aaiaoeea. — 1997. — O.31, N 1. — N.16-20.

2. Aa?aeoeeay O., Eiaeooiaa I., Aoaeaa Ae., Neaieai TH., Nieieia
A.Aeeooa?aioeeaoeey eeiee eoeo?ocu i?e iiiiue iieaeoey?iuo ia?ea?ia. //
Eoeo?oca e ni?ai. — 1997. — N 6. — N.7-11.

3. Eiaeooiaa I., Nieiaeaiei A., Neaieai TH. Ianeaaeiaaiea
i?iaeoeoia aiieeoeeaoeee AeIE o F1-aea?eaeia eoeo?ocu e iiaenieia/ieea
// Oeeoieiaey e aaiaoeea. — 1998. — N 4. — N.26-31.

4. Sivolap Yu., Chebotar S., Kalendar R., Kozhuhova N. Inter and
intraspecies genome changeability of some cultivated cereals // Oacenu
VI Iaaeaeoia?iaeiie eiioa?aioeee «Frontiers in biochemostry and
molecular biology. The legacy of Andrey Belozersky». — Iineaa,
1995 a.

5. Eiaeooiaa I., Neaieai TH. Aiaeic iieiii?oicio aeiaaeeie
aiieioieiaaieo o?aaiaioia AeIE eoeo?oaece (Zea mays) // Iaoa?eaeu
iaaeaeoia?iaeiie iao/ii-i?aeoe/aneie eiioa?aioeee iieiaeuo o/aiuo e
niaoeeaeenoia «Ianeiaeee iaoeiaeo iiooeia iieiaeeo a/aieo-aa?a?ieeia
a oiiaao ?aoi?ioaaiiy AIE». — *aaaie, 1996. — N.222.

6. Eiaeooiaa I., Neaieai TH., Aa?aiee A. Annioeeaoeee i?iaeoeoia
aiieeoeeaoeee AeIE n eieonaie, eiio?iee?othueie iaeioi?ua oicyenoaaiii
oeaiiua i?eciaee eoeo?ocu // Oacenu aeieeaaeia iaaeaeoia?iaeiie
eiioa?aioeee «Aa?iaeioaoiieiaee ?anoaiee e aeeaioiuo». — Eeaa, 1997. —
N.21-22.

7. Eiaeooiaa I. Iieeia?aciay oeaiiay ?aaeoeey e iniaaiiinoe aa
i?eiaiaiey aeey aiaeeca iieeii?oecia // Eniieueciaaiea
IOe?-aiaeeca a aaiaoeei-naeaeoeeiiiuo enneaaeiaaieyo.
Iao/ii-iaoiaee/aneia ?oeiaiaenoai. Iiae ?aae. TH.I.Neaieaia. — Eeaa:
Aa?a?ia iaoea, 1998. – 156 c.

8. Eiaeooiaa I., Aa?aeoeeay O. Iieaeoey?ii-aaiaoe/aneee iieeii?oeci
eoeo?ocu // Eniieueciaaiea IOe?-aiaeeca a aaiaoeei-naeaeoeeiiiuo
enneaaeiaaieyo. Iao/ii-iaoiaee/aneia ?oeiaiaenoai. Iiae ?aae.
TH.I.Neaieaia. — Eeaa: Aa?a?ia iaoea, 1998. – 156c.

Eiaeooiaa I.N. Aeine?aeaeaiiy iieaeoey?ii-aaiaoe/iiai
iie?ii?o?cio aaiiia eoeo?oaece iaoiaeii iie?ia?acii? eaioethaiai?
?aaeoe??.- ?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa
a?ieia?/ieo iaoe ca niaoe?aeuei?noth 03.00.26-iieaeoey?ia aaiaoeea. —
?inoeooo iieaeoey?ii? a?ieia?? e aaiaoeee IAI Oe?a?ie, Ee?a, 1998

Aeena?oaoeiy i?ena’y/aia ieoaiiyi aeei?enoaiiy iniiaieo
iiaeoiaeia AeIE-i?ioiethaaiiy (aeiaieueii i?aeiiaaia-IE? oa SSR-IE?)
aeey aeineiaeaeaiiy iieaeoey?ii-aaiaoe/iiai iieiii?oicio eoeo?oaece,
cie?aia, aeey iaeaioeoieaoei§ oa aeeoa?aioeiaoeii aaiioeiia.
I?iaaaeaii ii?iaiyeueiee aiaeic aeene?eiiiaoeieiii neee
aiioiii/ieo oa iieaeoey?ieo ia?ea?ia. Aea/aii oa?aeoa? oniaaeeoaaiiy
aiieieiiia, io?eiaieo ?icieie iaoiaeaie IE?. Cai?iiiiiaaia oano-iaiaeue
ic oanoe SSR-eieonia aeey aecia/aiiy «aia?eaeiinoi» i?inoeo aia?eaeia
eoeo?oaece. I?iaaaeaii ei?aeyoeieiee aiaeic aiieieiiia i aa?iiiii/ii
aaaeeeaeo iciae ca eieueeinieie iieacieeaie aeaio ?ieia.

Eeth/ia? neiaa: aaiaoe/iee iieiii?oeci, IE?, eoeo?oaeca

Eiaeooiaa I.Y. Enneaaeiaaiea iieaeoey?ii-aaiaoe/aneiai
iieeii?oecia aaiiia eoeo?oaecu (Zea mays L.) iaoiaeii iieeia?aciie
oeaiiie ?aaeoeee.- ?oeiienue.

Aeenna?oaoeey ia nieneaiea o/?iie noaiaie eaiaeeaeaoa
aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.26 — iieaeoey?iay aaiaoeea.-
Einoeooo iieaeoey?iie aeieiaee e aaiaoeee IAI Oe?aeiu, Eeaa, 1998

Aeenna?oaoeey iinayuaia aii?inai eniieueciaaiey iniiaiuo
iiaeoiaeia AeIE-i?ioeee?iaaiey (II-IOe? e SSR-IOe?) aeey enneaaeiaaiey
iieaeoey?ii-aaiaoe/aneiai iieeii?oecia eoeo?ocu, a /anoiinoe, aeey
eaeaioeoeeaoeee e aeeooa?aioeeaoeee aaiioeiia. I?iaaaeai n?aaieoaeueiue
aiaeec aeene?eieiaoeeiiiie neeu aeioeie/aneeo e iieaeoey?iuo
ia?ea?ia. Eco/ai oa?aeoa? ianeaaeiaaiey aiieeeiiia, iieo/aiiuo
?acee/iuie iaoiaeaie IOe?. I?aaeeiaeaia oano-iaiaeue ec oanoe
SSR-eieonia aeey ii?aaeaeaiey «aea?eaeiinoe» i?inouo aea?eaeia
eoeo?ocu. I?iaaaeai ei??aeyoeeiiiue aiaeec aiieeeiiia e aa?iiiie/anee
aaaeiuo i?eciaeia ii eiee/anoaaiiui iieacaoaeyi aeaoo eao.

Eeth/aaua neiaa: aaiaoe/aneee iieeii?oeci, IOe?, eoeo?oca

Kozhuhova N. Molecutar-genetic polymorphism
investigation of maize (Zea mays L.) gemone by polymerase chain
reaction.- Handcopy.

Thesis for the Degree of Candidate of Biological sciences
on speciality 03.00.26 — molecular genetics. — Molecular biology and
genetics institute NAS, Ukraine. Kiev, 1998

The dissertation was devoted to various DNA-profiling
techniques (AP-PCR and SSR-PCR) usage for molecular-genetic
polymorphisms investigation in maize. Genotype identification and
differentiation were elaborated. Comparative analysis for
discriminative power of biochemical and molecular markers was carried.
The character of inheritance of amplicons developed by various
PCR-methods was studied. The test-pannel of six SSR-loci was suggested
for «hybridity» determination of simple maize hybrids. Cirrelative
analysis of amplicons and agronomically important traits based on
quantitative indices for two years was carried.

Key words: genetic polymorphysm, PCR, maize

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