12

IIIINOA?NOAI INAIOE OE?A?IE

OA?NIINUeEEE AeA?AEAAIEE IAAeAAIAI*IEE OIIAA?NEOAO

IIEAUOE Naaiia Aieoi?iaia

OAeE 581.1:581.4 + 581.84

AIIEIAI*IA AEOEAIINOUe AIOEIENEAeAIOIA

A EOEUeOO?I OEAIEI OIIAOA IN VITRO

03.00.20 — aiioaoiieiaiy

AAOI?AOA?AO

aeena?oaoei? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa aiieiai/ieo iaoe

Oa?nii — 1998

Aeena?oaoei?th ? ?oeiien.

?iaioa aeeiiaia o Ana?inienueeiio iinoeoooi nieuenueeiainiiaea?nueei?
aiioaoiieiai? ?inienueei? Aeaaeaii? nieuenueeiainiiaea?nueeeo iaoe.

Iaoeiaee ea?iaiee: aeieoi? aiieiai/ieo iaoe, noa?oee iaoeiaee
niia?iaioiee Ai?aaoaiei Iai? TH?ieiae/, Oa?niinueea aiaeaeieaiiy
Iinoeoooo nieuenueeiainiiaea?nueei? aiioaoiieiai?, caaiaeoaa/

Ioioeieii iiiiaioe: aeieoi? aiieiai/ieo iaoe, noa?oee iaoeiaee
niia?iaioiee Eaaaiei Ai?en Ieaenieiae/, Iinoeooo oiciieiai? ?ineei i
aaiaoeee IAI Oe?a?ie, caaiaeoaa/ aiaeaeieii

eaiaeeaeao aiieiai/ieo iaoe ?oaean Aieiaeeie? Aiae?ieiae/, Iinoeooo
eeioeiii? aiieiai? oa aaiaoe/ii? iiaeaia?i? IAI Oe?a?ie, iaoeiaee
niia?iaioiee

I?iaiaeia onoaiiaa: Iaoeiiiaeueiee aa?a?iee oiiaa?neoao Eaa?iaoo
I?i?no??a Oe?a?ie, eaoaae-

?a naeaeoe?? oa o?c?ieia?? ?ineei, i.
Ee?a

Caoeno aiaeaoaeaoueny «22» aeiaoiy 1998 ?. i 14 aiaeeii ia caniaeaiii
niaoeiaeiciaaii? a/aii? ?aaee E67.053.01 i?e Oa?niinueeiio aea?aeaaiiio
iaaeaaiai/iiio oiiaa?neoaoi ca aae?anith: 325000, i.Oa?nii, aoe. 40
?ieia AEiaoiy, 27.

C aeena?oaoei?th iiaeia iciaeiieoenue o aiaeiioaoei Oa?niinueeiai
aea?aeaaiiai iaaeaaiai/iiai oiiaa?neoaoo, aoe. 40 ?ieia AEiaoiy, 27.

Aaoi?aoa?ao ?icineaii «19» aeiaoiy 1998 ?ieo.

A/aiee nae?aoa?

niaoeiaeiciaaii? a/aii? ?aaee
A.I.Aoa/aiei

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

AEOOAEUeIINOUe OAIE. Eoeueoeaoaaiiy ?ineeiieo aenieaioia in vitro ia
ooo/ieo aeeaeeueieo na?aaeiaeuao ? iniiaith aaaaoueio oaoiieiaie, yei
ni?eythoue cia/iiio i?enei?aiith naeaeoeieiiai i?ioeano: eeiiaeueiiai
iie?i?iciiiaeaiiy, iaea?aeaiiy aia?eaeia i?e ianoiiniinoi aeoiaeieo
oi?i, noai?aiiy aaiei?aeieo oa aeeaaiei?aeieo eiiie, niiaoe/iiai
aia?iiaaiaco, aeaeieaiiy niiaeeiiia, eeioeiii? naeaeoei? i o. ii.
Aoaeoeaiinoue oeeo oaoiieiaie cia/iith ii?ith caeaaeeoue aiae
aeei?enoaiiy a neeaaei aeeaeeueieo na?aaeiaeu aiieiai/ii aeoeaieo
?a/iaei, yei caeaoii aieeaaoe ia iai?yiie ?inoo aenieaioia. Icieueiaaii
i?aaie, oeaieie oa eeioeie ?ineei oaeiae ? ia’?eoii aiioiii/ieo oa
oiciieiai/ieo aeineiaeaeaiue, a oiio /enei aecia/aiiy oiia oa iaoaiiciia
aei? ?aaoeyoi?ia ?inoo ia iaoaaiei/ii i?ioeane.

I?e ?icaeyaei oiciieiai/ieo iniaeeainoae aenieaioia ii?iaiyii c
iioaeoieie ?ineeiaie iaiaoiaeii a?aoiaoaaoe iiaeeeai ianeiaeee
iaoaii/iiai iioeiaeaeaiiy iiae /an iciethaaiiy. Salin & Briges, 1981,
iieacaee, ui iaoaii/ia iioeiaeaeaiiy ?ineeiieo oeaiei noi?iaiaeaeo?oueny
iioaineoieaoei?th aieueii?aaeeeaeueieo i?ioeania. Iaeii/anii
niinoa?iaa?oueny aeoiae oaiieia c aaeoieae a ieacio oa ?o ieeneaiiy
eieaeiciaaieie oai oa?iaioaie c ooai?aiiyi iiaiaoth/eo ?ino i?iaeoeoia
(Ae.E.Noii, 1972). Oaeei /eiii, ?a/iaeie c aioeieneaeaioieie aai
aiaeiiaieie aeanoeainoyie iiaeooue aooe aeei?enoaii aeey iiaeaeuaiiy
aeeoo?caeaoiinoi aenieaioia.

?a/iaeie-aiaeiiaieee: anei?aiiiaa eeneioa, aeooaoiii-SH oa iioi, —
aoiaeyoue aei neeaaeo aeayeeo aiaeiieo aeeaeeueieo na?aaeiaeu (Reinert &
White, 1956; Kao, Michaylyk, 1975; Nitsch, Asahira et al., 1970;
Lescure, 1966 oa ii.), a oaeiae aeei?enoiaothoueny aeey ia?iaee
aenieaioia aaciina?aaeiuei iiney iciethaaiiy aai iiae /an
noaeoeueoeaoaaiiy (Meyer et al., 1975; Monaco et al., 1977). Iniiaia
oaaaa aei oaia?ioiueiai /ano i?eaeiey?oueny caoenoo aenieaioia aiae
iaaeii?iiai ooai?aiiy i?iaeoeoia oa?iaioaoeaiiai ieeneaiiy oaiieueieo
niieoe. Iaaaoeaia aeiy aieueii?aaeeeaeueiiai ieeneaiiy oa aieea inoeiieo
aioeieneaeaioia-iiaiaioi?ia ia iaoaaiei/ii i?ioeane, ?aaoeyoeiy yeeo
iia’ycaia ic nenoaiith i?i- e aioeieenethaa/ia a eoeueoo?i ?ineeiieo
oeaiei in vitro i?aeoe/ii ia aeineiaeaeaii. Ia aecia/aii oaeiae
iniaeeainoi aieeao ia ?ino oa ?icaeoie aenieaioia aioeieneaeaioia,
?icieo ca iaoaiiciii aei? (aioe?aaeeeaeueii aaaioe oa
?a/iaeie-aiaeiiaieee) i iioiaeaeaiiyi (neioaoe/ii oa i?e?iaeii).

Oaeei /eiii, iniothoue aeinoaoiuei aaaiii ia?aaeoiiae, ui aeacothoue ia
iaiaoiaeiinoue aecia/aiiy aiieiai/ii? aeoeaiinoi i?e?iaeieo oa
neioaoe/ieo aioeieneaeaioia a eoeueoo?i ?ineeiieo oeaiei in vitro. Oei
aeineiaeaeaiiy iiaeooue oaeiae ni?eyoe iiaeaeueoiio aea/aiith ?iei
aieueii?aaeeeaeueiiai ieeneaiiy aiiiieaeoe a ooieoeiiioaaiii ?ineeiieo
i?aaiiciia.

CA’ssCIE ?IAIOE C IAOEIAEIE I?IA?AIAIE. Aeineiaeaeaiiy ca oaiith
aeena?oaoei? i?iaiaeeeeny o aiaeiiaiaeiinoi aei ieaiia Ana?inienueeiai
iaoeiai-aeineiaeiiai iinoeoooo nieuenueeiainiiaea?nueei? aiioaoiieiai?
?inienueei? Aeaaeaii? cieuenueeiainiiaea?nueeeo iaoe. ?iaioa ?
neeaaeiaith /anoeiith oaie «Aeineiaeaeaiiy iaoaiiciia aei? ?aaoeyoi?ia
?inoo» (iiia? aea?aeaaii? ?a?no?aoei? 01.9.60 002370).

IAOA AeINEIAeAEAIUe. Iaoith aeineiaeaeaiue aoei aecia/aiiy iiaeeeainoi
aeei?enoaiiy aioeieneaeaioia, ?icieo ca iioiaeaeaiiyi (i?e?iaeii oa
neioaoe/ii) oa iaoaiiciii aei? (aioe?aaeeeaeueia oa aiaeiiaia), ye
?aaoeyoi?ia ?inoo a eoeueoo?i oeaiei oiiaoa in vitro c o?aooaaiiyi
iniaeeainoae ?icieo oeiia aenieaioia.

CAAeA*I ?IAIOE

1. Aeineiaeeoe aieea aaaaeaiiy aei aeeaeeueiiai na?aaeiaeua aeey
aenieaioia oiiaoa aioeieneaeaioia (neioaoe/iiai — oaiienaia, i?e?iaeieo
— anei?aiiiai? eeneioe, aeooaoiiia-SH, -oieioa?iea) ia aiiiao?e/ii
iieacieee: iano ?ineeie-?aaaia?aioa aai eaeona ci noi?iiaaieie iaaiiaie
oa ei?iiiyi, aenioo iaaiia, aeiaaeeio ei?aiia, a oaeiae iioaineaiinoue
ei?aiaooai?aiiy oa /anoioo i?aaiiaaiaco.

2. Aecia/eoe caeaaeiinoue aei? oaiienaia ia aiieaeueii iie?iaeeaoei
oiiaoa aiae ianooiieo oaeoi?ia:

o?eaaeinoi eoeueoeaoaaiiy aenieaioia ia na?aaeiaeuao c aioeieneaeaioii;

aaiioeio ?ineeie;

iayaiinoi o neeaaei na?aaeiaeua FeSO4 oa Na2AAeOA; aoeneiia.

3. Ii?iaiyoe aeith oaiienaia ia ?ino iie?iaeeaoeia oa anaioe/ieo
i?i?inoeia oiiaoa.

4. Aeineiaeeoe aieea anei?aiiiai? eeneioe ia eaeoniaaiac i ianooiio
?aaaia?aoeith o aenieaioia nii’yaeieae oa aeiieioeeia oiiaoa a no?aniaeo
oiiaao (i?e cieaeaiie oaiia?aoo?i eoeueoeaoaaiiy).

IAOEIAA IIAECIA. A ?acoeueoaoi i?iaaaeaii? ?iaioe aoea anoaiiaeaia
caeaoiinoue aioeieneaeaioia-iiaiaioi?ia noeioethaaoe i?ioeane ?inoo oa
?aaaia?aoei? a eoeueoo?i oeaiei oiiaoa in vitro. Iaea?aeaii aeaii
iiaeoaa?aeaeothoue iieiaeaiiy i?i iayaiinoue o aioeieneaeaioia ia
oieueee aioeno?aniaeo, a e ?ino?aaoethth/eo aeanoeainoae. Aia?oa
iieacaia iiaeeeainoue aeei?enoaiiy aeey noeioeyoei? ?inoo iie?iaeeaoeia
neioaoe/iiai aioeieneaeaioa oaiieueii? i?e?iaee oaiienaia, yeee a
oeueiio aeiaaeeo nooo?ai ia?aaeuoaaa ii aeoeaiinoi o?aaeeoeieii aaeeaaii
anei?aiiiao eeneioo oa aeooaoiii-SH. Aecia/aia caeaaeiinoue aoaeoo
oaiienaia aiae oeio aenieaioa, aaiioeio ?ineeie, eiioeaio?aoei?,
aeniiceoei?, iayaiinoi a aeeaeeueiiio na?aaeiaeui aoeneiia oa Fe-oaeaoa.

I?AEOE*IA CIA*AIIss IAeA?AEAIEO ?ACOEUeOAOIA. Iaea?aeaii ?acoeueoaoe
naiae/aoue i?i aeioeieueiinoue ?icoe?aiiy aeei?enoaiiy aioeieneaeaioia o
neeaaei aeeaeeueieo na?aaeiaeu aeey eoeueoeaoaaiiy ?ineeiieo aenieaioia
in vitro. A oaoiieiaiyo eeiiaeueiiai iie?i?iciiiaeaiiy iaeaieueo
aoaeoeaiei iiaea aooe neioaoe/iee aioeieneaeaio oaiienai, aeey yeiai
oa?aeoa?ia noaaieueia ?inonoeioethth/a aeiy, ui i?iyaey?oueny o
caieueoaiii eieueeinoi ei?aiia, iane ?ineei, aenioe iaaiia o
?aaaia?aioia. Aenia?eiaioaeueii aeaii c aea/aiiy aiieiai/ii? aeoeaiinoi
aioeieneaeaioia, ?icieo ca iaoaiiciii aei?, iiaeathoue iaoa?iae aeey
iiaeaeueoiai aeineiaeaeaiiy aieueii?aaeeeaeueiiai oa oa?iaioaoeaiiai
ieeneaiiy a ?icieo aenieaioao a ca’yceo c ?o ii?oi-oiciieiai/ieie
iniaeeainoyie.

INIAENOEE AIANIE AeENA?OAIOA. Aeena?oaio iniaenoi i?i?iaeea iaiaoiaeio
eioa?aoo?o, iaieiaeiea iaoiaeeeith eoeueoeaoaaiiy ?ineeiieo oeaiei in
vitro, iinoaiiaee aeineiaeia oa noaoenoe/ii? ia?iaee ?o ?acoeueoaoia,
i?eeiaea aeoeaio o/anoue a ieaioaaiii aeineiaeaeaiue, iiaeaioiaoei
iaoa?iaeia aei ae?oeo. Aenia?eiaioaeueii aeaii, iieeaaeaii a iniiao
?iaioe, iaea?aeaii aeena?oaioii iniaenoi. A ioaeieaoeiyo iniaenoee
aianie aeena?oaioa neeaaea? 50-90%.

AI?IAAOeIss ?ACOEUeOAOIA AeENA?OAOeI?. Iaoa?iaee ?iaioe aeiiiaiaeaeenue
ia 2-e eiioa?aioei? «?aaoeyoi?u ?inoa e ?acaeoey ?anoaiee» (Iineaa,
1993), 2-iio Aaeoienueeiio aaiaoe/iiio eiia?ani (TH?iaea, 1995),
eiioa?aioei? «Aeooaeueiua i?iaeaiu aeioaoiieiaee a ?anoaieaaiaenoaa,
aeeaioiiaiaenoaa e aaoa?eia?ee» (Iineaa, 1996), Iiaeia?iaeiiio
?aaiiiaeueiiio naiiia?i «Environment Protection: Modern Studies in
Ecology and Microbiology» (Oaeai?iae, 1997).

IOAEIEAOeI?. Iaoa?iaee aeena?oaoei? iioaeieiaaii a 8 ?iaioao, a oiio
/enei 3 noaooyo a iaoeiaeo aeo?iaeao, 1 noaooi a cai?ieeo iaoeiaeo
i?aoeue, a oaeiae a 4 oacenao eiioa?aioeie.

NO?OEOO?A AeENA?OAOeI?. Aeena?oaoeiy neeaaea?oueny ic anooio, 4
?icaeieia, aeniiaeia. Ianya ?iaioe — 127 noi?iiie iaoeiiieniiai oaenoo,
a oiio /enei 17 oaaeeoeue, 6 iethno?aoeie, nienie eioa?aoo?e ia 16
noi?iieao (138 aeaea?ae).

IAOIAeEEA AeINEIAeAEAIUe

Caaaeueia noaia aeineiaeaeaiue iaaaaeaia ia ?en.1. A ?iaioi aoee
aeei?enoaii oaei ?a/iaeie: oaiienai (neioaoe/iee aioeieneaeaio, iaaeaiee
Iinoeoooii oiii/ii? oiceee ?AI), anei?aiiiaa eeneioa, oieioa?ie,
aeooaoiii-SH. Ia’?eoii aeineiaeaeaiue aoee aenieaioe anaioe/ieo
i?i?inoeia oiiaoa (Lycopersicon esculentum Mill., ni?o Iiai/ie), a
oaeiae naii i?i?inoee. Aeiaeaoeiai aea/aeeny ni?oiai aiaeiiiiinoi
?aaeoei? iie?iaeeaoeia ia aaaaeaiiy o na?aaeiaeua oaiienaia.

I?eaiooaaiiy aeeaeeueieo na?aaeiaeu, noa?eeicaoeiy aeoiaeiiai iaoa?iaeo,
iciethaaiiy oa ae?iuoaaiiy aenieaioia caeienithaaeinue ca
caaaeueiii?eeiyoeie iaoiaeeeaie. A ?iaioi aeei?enoiaoaaeeny oaei oeie
aenieaioia: iie?iaeeaoei — aiae?icee iaaiia aeiaaeeiith i?eaeecii 12 ii
c aiieaeueiith a?oiueeith (A) aai iacooiaeie a?oiueeaie nii’yaeieae
(IAN), a oaeiae aiae?icee aiiieioeey aeiaaeeiith i?eaeecii 10 ii (A) oa
o?aaiaioe nii’yaeieae.

Iniiaith anio aeeaeeueieo na?aaeiaeu aoei aaa?eciaaia na?aaeiaeua
Io?aniaa-Neoaa (1962 ?.), aeey A i IAN — aac oioiai?iiiia, aeey N i A —
c 1 ia/e IIE oa 2 ia/e AAI. A iniiaia na?aaeiaeua, caiaeii ci noaiith
aeineiaeo, aeiaeaaaany iaeei c aeineiaeaeoaaieo aioeieneaeaioia.
Aenieaioe eoeueoeaoaaeeny i?e 16-aiaeeiiiio oioiia?iiaei i?ioyaii 4 (A i
IAN) aai 6 (N i A) oeaeiia. ?o ?ino oa ?icaeoie ioeiithaaeenue ii oaeei
aiiiao?e/iei iieacieeai: aenioa iaaiia, iaeneiaeueia aeiaaeeia ei?aiy,
eieueeinoue ei?aiia, iana ?ineeie aai eaeona c iaaiiaie oa ei?aiyie,
/anoioa i?aaiiaaiaca. Noaoenoe/ia ia?iaea aeaieo iieyaaea o aecia/aiii
na?aaeiuei? aaee/eie iciaee, na?aaeiueieaaae?aoe/iiai aiaeoeeaiiy,
eiaoioei?ioa aa?iaoei?, iiieeee na?aaeiuei?. Aeinoiai?iinoue na?aaeiio
aaee/ei i ?icieoei iiae aeineiaeieie oa eiio?ieueieie aa?iaioaie
ioeiithaaeanue ca e?eoa?i?i No’thaeaioa. A aeineiaeao c aenieaioaie A oa
i?i?inoeaie aeiaeaoeiai ia/enethaaeenue ia?ii eiaoioei?ioe ei?aeyoei?
iiae oniia iieacieeaie.

?ACOEUeOAOE OA ?O IAAIAI?AIIss

AIEEA OAIIENAIA IA ?INO AIIEAEUeIEO IIE?IAEEAOeIA OIIAOA. ?acoeueoaoe
aeineiaeaeaiiy aieeao oaiienaia ia aiiiao?e/ii iieacieee ?aaaia?aioia c
aiaenia iaaaaeaii a oaae. 1. Noeioethth/a aeiy oei?? ?a/iaeie
i?iyaeyeany a caeaoiinoi aeeeeeaoe aeinoiai?ia caieueoaiiy iane
?ineei-?aaaia?aioia i eieueeinoi ei?aiia, o aeayeeo aeiaaeeao — oaeiae
aenioe iaaiia oa iaeneiaeueii? aeiaaeeie ei?aiy. Iioeiaeueieie aeey
ni?oo Iiai/ie aoee eiioeaio?aoei? oaiienaia 10-15 ia/e.

Oaaeeoey 1

Aieea oaiienaia ia aiiiao?e/ii iieacieee ?aaaia?aioia c aiieaeueieo
iie?iaeeaoeia oiiaoa (a na?aaeiueiio ii /ioe?ueii aeineiaeai)

Iieacieee,Eiio?ieueEiioeaio?aoeiy oaiienaia, ia/e% aei
eiio?ieth5101520Iana?ineeie100,0128,3*134,4*132,9*142,4*Aenioa
iaaiia100,0111,7*108,2104,6102,1Iaeneiaeueiaaeiaaeeia
ei?aiy100,0100,4100,196,894,6Eieueeinoueei?aiia100,0132,0150,8*168,1*190
,6**I?eiioea. ?icieoey iiae aeineiaeiei oa eiio?ieueiei aa?iaioaie
aeinoiai?ia i?e: *P=0,95; **P=0,99.

Aiaeic aeeiaiiee ?inoo ?ineei (?en. 2) iieacaa, ui ia?oith
niinoa?aaeoaaiith ?aaeoei?th aenieaioia ia oaiienai, ye i?aaeei, aoei
caieueoaiiy eieueeinoi ei?aiia, yea niinoa?iaaeinue aaea a 7-aeaiiiio
aioei. Ia?aaeuaiiy eiio?ieueieo iieacieeia ii aenioi iaaiia c’yaeyeinue
iiciioa, eiai aaee/eia a aieueoinoi aeiaaeeia aeinyaaea iaeneioia a
eiioei 2 oeaeiy eoeueoeaoaaiiy, a iioii aeaui ciaiooaaeanue. Oaeei
/eiii, ?inonoeioethth/ee aoaeo oaiienaia a eoeueoo?i aiaenia oiiaoa in
vitro iia’ycaiee, eiiai?ii, c iioaineoieaoei?th ei?aiaooai?aiiy ia
ii/aoeo ia?iiaeo ae?iuoaaiiy. A oie aea /an, aeineiaeaeaiiy aoaeoo ei-
?ioei/aniiai eoeueoeaoaaiiy ia na?aaeiaeuao c oaiienaiii iieacaei, ui
aeey i?iyaeaiiy noeioethth/i? aei? oeueiai aioeieneaeaioa o?eaaeinoue
eiai aieeao iiaeiia aooe ia iaioith 5 aeia.

Caieueoaiiy eiioeaio?aoei? oaiienaia aei 30 ia/e i aeua i?eaiaeeei aei
ooai?aiiy iae?icia. I?ioyaii ia?oiai oeaeiy eoeueoeaoaaiiy ia oaeeo
na?aaeiaeuao ieaeiie eiiaoeue aenieaioa (aeieyiea aenioith 1-3 ii)
iioiiooaaany, iaaoaaa aeiaooaaoiai, a iioii ao?iai caaa?aeaiiy. A
iiaeaeueoiio oey aeieyiea iiaea iiaiinoth anioiooe. Iaeii/anii
niinoa?iaaeiny ooai?aiiy i?eaeaoeiaeo ei?aiia c iaiioeiaeaeaii? /anoeie
noaaea, ?ino iaaiia i?ioiaeea ii?iaeueii. A ?acoeueoaoi oaei ?ineeie
ia?iaeei ia?aaeuoaaee eiio?ieueii ii aiiiao?e/iei iieacieeai.

Aeey ii?iaiyiiy ?aaeoei? ?icieo aaiioeiia oiiaoa ia aeiaeaaaiiy
oaiienaia a aeeaeeueia na?aaeiaeua aea/aee ?ino oa ?icaeoie aenieaioia A
anaioe/ieo i?i?inoeia ni?oia Iiai/ie, Aieaia?aaenueeee 5/95, Oaeae,
NO-1, NO-2 oe eiii? 629. Eiioeaio?aoei? oaiienaia (10 oa 15 ia/e) aoee
aea?aii ca ?acoeueoaoaie aeineiaeia ic ni?oii Iiai/ie. I?e aaaaeaiii
oaiienaia a na?aaeiaeua caieueoaiiy aiiiao?e/ieo iieacieeia
niinoa?iaaeiny o ni?oia Iiai/ie, NO-1 i NO-2, i?e oeueiio aeey Iiai/ea
oa NO-1 e?auith aoea eiioeaio?aoeiy aioeieneaeaioa 15 ia/e, aeey NO-2 —
10 ia/e. O ni?oo Aieaia?aaenueeee 5/95 i eiii? 629 aeinoiai?ieo
aiaeiiiiinoae iiae aa?iaioaie ia aeyaeaii. O aenieaioia ni?oo Oaeae
i?iyaeany iae?iaaiiee aoaeo oaiienaia, ui i?eaaei aei oiiaieueiaiiy
oaiiia ?inoo iaaiia oa ei?aiia.

CAEAAEIINOUe ?INONOEIOETHTH*I? AeI? OAIIENAIA AIAe I?ENOOIINOI A
NA?AAeIAEUI Fe-OAEAOA. C eiiiiiaioia aeeaeeueiiai na?aaeiaeua
Io?aniaa-Neoaa iniaeeaee iioa?an aeey aeineiaeaeaiiy
i?i-aioeieenethaaeueii? ?iaiiaaae iathoue FeSO4, ineieueee iiie Fe2+
i?eeiathoue o/anoue a aaia?aoei? aieueieo ?aaeeeaeia, oa Na2AAeOA —
oaeaooth/ee aaaio, yeee oiiaieueith? ieeneaiiy oeeo iiiia.

?acoeueoaoe aeineiaeaeaiiy aieeao oaiienaia (20 ia/e) ia oiii
aeeeth/aiiy ci neeaaeo na?aaeiaeua iaeiiai aai iaio eiiiiiaioia
Fe-oaeaoa ia aiiiao?e/ii iieacieee ?aaaia?aioia c aiaenia oiiaoa
iaaaaeaii ia ?en. 3. A iaoiio aenia?eiaioi ?ineeie, iaea?aeaii ia
na?aaeiaeui aac Na2AAeOA, aiae?iciyeeny aiae eiio?ieueieo oieueee
iaioith aeiaaeeiith ei?aiia. Aaaaeaiiy aioeieneaeaioa a oeueiio aeiaaeeo
ia oieueee ia iaei noeioethth/iai aoaeoo, aea e, iaaiaee, ?icei
oiiaieueieei ?ino ei?aiia oa iaaiiia, ui i?eaaei aei cia/iiai cieaeaiiy
iane ?ineeie (ia 62,3%). Iiaeeeai, oiene/iei aeey ?ineei ? ia aeania
oaiienai, a i?iaeoeoe eiai ieeceaiiy, yea iiaeiia cia/ii iineethaaoeny o
aiaenooiinoi oaeaooth/iai aaaioa. Aeeeth/aiiy c aeeaeeueiiai na?aaeiaeua
FeSO4 a onio aa?iaioao noi?iaiaeaeoaaeiny cia/iei iiaiaoaaiiyi ?inoo oa
?icaeoeo aenieaioia, i?e oeueiio oaiienai aeinoiai?ii iiaeaeuoaaa
aiiiao?e/ii iieacieee ?ineei.

Iaiaoiaeii aiaecia/eoe, ui caieueoaiiy eieueeinoi ooai?aieo ia aenieaioi
ei?aiia niinoa?iaaeinue a onio aa?iaioao c oaiienaiii, iacaeaaeii aiae
iayaiinoi aai aiaenooiinoi a na?aaeiaeui eiiiiiaioia Fe-oaeaoa.

AeIss I?E?IAeIEO AIOEIENEAeAIOIA IA IIE?IAEEAOeI OIIAOA. Aeaii i?i
eiioeaio?aoei? i?e?iaeieo aioeieneaeaioia, ui i?iyaeee aeinoiai?iee
aieea ia aiiiao?e/ii iieacieee ?aaaia?aioia c aiaenia oiiaoa, iaaaaeaii
a oaaeeoei 2. Iaeaieueo neeueiith ?inonoeioethth/ith aei?th
oa?aeoa?ecoaaany -oieioa?ie, yeee, ia aiaeiiio aiae oaiienaia,
caieueooaaa aeiaaeeio ei?aiia aac ciii o ?o eieueeinoi. Iiceoeaiee aoaeo
oeueiai aioeieneaeaioa i?iyaeyany aaea i?e eiioeaio?aoei? 1 ia/e, a
iiaeaeuaiiy aeice aei 100 ia/e ia aeeeeeaei iaaaoeaieo ianeiaeeia.

Aeooaoiii-SH e anei?aiiiaa eeneioa aieeaaee iiceoeaii oieueee ia ie?aii
aiiiao?e/ii iieacieee ?aaaia?aioia c aiaenia oiiaoa. E?ii oiai, ?o aeiy
oa?aeoa?ecoaaeany

Oaaeeoey 2

Aieea i?e?iaeieo aioeieneaeaioia ia ?ino oa ?icaeoie

aiaenia oiiaoa in vitro

AioeieneaeaioEiioeai-Aeinoiai?ii aiaeiiiiinoi aiae eiio?ieth,%o?aoeiy,
ia/eIana ?ineeieAenioa iaaiiaIaen.

aeiaaeeia ei?aiyEieueeinoue

ei?aiia-Oieioa?ie1—22,3*-5—22,4*-5037,5**11,5*
28,2**-10032,5**—Aeooaoiii-SH100—12,2*-Anei?aiiiaa1034,9**—Eeneioa1
000-69,8***-56,4***-87,5***49,2**

I?eiioea. ?icieoey iiae aeineiaeiei oa eiio?ieueiei aa?iaioaie
aeinoiai?ia i?e: *?=0,95; **?=0,99; ***?=0,999.

iecueeith iiaoi?thaaiinoth. A eiioeaio?aoei? 1000 ia/e anei?aiiiaa
eeneioa ?icei iiaiaoaaea ?ino iaaiia oa ei?aiia, aea eieueeinoue ei?aiia
i?e oeueiio caieueooaaeanue ia 50%. Iecueea aoaeoeaiinoue aeei?enoaiiy
?a/iaei-aiaeiiaieeia o neeaaei na?aaeiaeu aeey iie?iaeeaoeia oiiaoa
naiae/eoue i?i aiaenooiinoue cia/ii? iaaaoeaii? aei? oa?iaioaoeaiiai
ieeneaiiy oa eiai i?iaeoeoia. Iie?iaeeaoei, ye i iioi iieiaei oeaieie,
ui aeoeaii ?inoooue, iinoyoue cia/io eieueeinoue aiaeiaaiieo
aeooaoiiia-SH oa anei?aiiiai? eeneioe e, iiaeeeai, ia iio?aaothoue ?o
iaaeoiaeaeaiiy ciaii.

AIEEA OAIIENAIA IA ?INO ANAIOE*IEO I?I?INOEIA OIIAOA. Aeineiaeaeaiiy
aiiiao?e/ieo iieacieeia anaioe/ieo i?i?inoeia iieacaei, ui a ieo,
iiaeiaii aei aenieaioia A, ia na?aaeiaeuao c oaiienaiii caieueoo?oueny
eieueeinoue ei?aiia oa iana ?ineeie i?e iacia/iiio iiaeaeuaiii aenioe
iaaiia oa cieaeaiii aeiaaeeie ei?aiy (oaae. 3).

I?e eiioeaio?aoei? oaiienaia 40 ia/e oa aieueoa niinoa?iaaeiny
iioaiiiiiy ia?aeiiiai ei?iioey, ?ino eiai oiiaieueithaaany aai
i?eieiyany, aea iioii ooai?thaaeenue ii?iaeueii i?eaeaoeiai ei?aii.
Iioeiaeueieie aeey ?inoo anaioe/ieo i?i?inoeia oiiaoa a iaoeo aeineiaeao
aoee eiioeaio?aoei? aioeieneaeaioa 10-20 ia/e. Oaeei /eiii,
?inonoeioethth/ee aoaeo oaiienaia i?iyaey?oueny ia oieueee ia
aenieaioao, a e ia iioaeoieo ?ineeiao i ia iiaea iiynithaaoeny aeeeth/ii
iineaaeaiiyi iaaaoeaieo ianeiaeeia iaoaii/iiai iioeiaeaeaiiy iiae /an
iciethaaiiy.

Oaaeeoey 3

?ino oa ?icaeoie anaioe/ieo i?i?inoeia oiiaoa

ia na?aaeiaeuao c oaiienaiii (Om)

Eiioe. oaiie-naia, ia/eIana i?i?inoea, aAenioa iaaiia, iiIaeneiaeueia
aeiaaeeia

ei?aiy, iiEieueeinoue

ei?aiia, oo.
00,34±0,01116,39±2,6664,36±1,954,67±0,20100,50±0,02***124,05±3,0064,94±2
,526,49±0,36***200,50±0,03***124,80±4,2250,20±2,41***6,43±0,37***300,42±
0,03*120,89±5,1442,38±2,67***6,57±0,48***400,24±0,03***92,40±3,93***24,1
4±2,32***5,11±0,42500,10±0,01***66,84±3,74***11,74±1,00***2,92±0,27***10
00,07±0,00***45,70±2,98***7,79±0,38***1,97±0,23***

I?eiioea. ?icieoey iiae aeineiaeiei oa eiio?ieueiei aa?iaioaie
aeinoiai?ia i?e: *?=0,95; **?=0,99; ***?=0,999.

AeIss AIOEIENEAeAIOIA IA ?INO OA ?ICAEOIE AENIEAIOIA OIIAOA IA
EAEONIAAIIIIO NA?AAeIAEUI. Aeinoiai?iiai caieueoaiiy /anoioe
eaeoniaaiaca i?e aaaaeaiii aei aeeaeeueiiai na?aaeiaeua aioeieneaeaioia
ia aeyaeaii. Iaeaieueo cia/iei iiceoeaiei aoaeoii ia /anoioo ooai?aiiy
iaaiiia oa eenoy c eaeono oa?aeoa?ecoaaany aeooaoiii-SH a eiioeaio?aoei?
1,74 ia/e (aeey aenieaioia N — 88,9% i?e eiio?iei 55,6%, aeey aenieaioia
A — 61,1% i?e eiio?iei 18,8%). Anei?aiiiaa eeneioa e -oieioa?ie
iiaeaeuoaaee /anoioo i?aaiiaaiaca aeineoue nooo?ai, aea oei aiaeiiiiinoi
ia aeinyaaee 5%-iiai ?iaiy cia/eiinoi. Oaiienai aeaui caieueooaaa
/anoioo ooai?aiiy iaaiiia oa eenoy o aenieaioia A.

Noeioethaaiiy ?inoo ei?aiia aioeieneaeaioaie caoieniaaii oieueee aeey
aenieaioia A: aeooaoiii-SH iiaeaeuoaaa eieueeinoue ei?aiia (1,74 ia/e —
8,0 oo., 17,4 ia/e — 7,5 oo. i?e eiio?iei 4,9 oo.), a oaiienai — ?o
aeiaaeeio (15,7 ia/e — 33,1 ii, 31,4 ia/e — 37,9 ii i?e eiio?iei 23,7
ii). A oie aea /an aeooaoiii-SH a eiioeaio?aoei? 174 ia/e cieaeoaaa ye
eieueeinoue ei?aiia, oae i ?o aeiaaeeio o iaio oeiia aenieaioia.

I?e aaaaeaiii aei aeeaeeueiiai na?aaeiaeua aioeieneaeaioia
niinoa?iaaeiny cia/ia caieueoaiiy iane eaeonia c ei?aiyie oa iaaiiaie,
iaea?aeaieo c aenieaioia A. I?e oeueiio iaeneiaeueia ia?aaeuaiiy iaae
eiio?ieai aiaecia/aeiny aeey anei?aiiiai? eeneioe a eiioeaio?aoei?
10 ia/e (65,8%) oa oaiienaia a eiioeaio?aoei? 15,7 ia/e (56,9 %). O
aeiaaeeo aenieaioia N caieueoaiiy iane niinoa?iaaeiny oieueee a
aeineiaeao c -oieioa?ieii, iaeneiaeueiei eiai cia/aiiyi (+31,9% aei
eiio?ieth) oa?aeoa?ecoaaeanue aeica 269 ia/e. Iaeneiaeueii aeice iioeo
aeineiaeaeaieo aioeieneaeaioia, iaaiaee, aeeeeeaee ?icea cieaeaiiy
oeueiai iieacieea.

Aeineiaeaeaiiy aieeao anei?aiiiai? eeneioe ia ?ino eaeonia oiiaoa i?e
iiieaeaiie oaiia?aoo?i eoeueoeaoaaiiy (20iN i 15iN) iieacaei, ui a oaeeo
oiiaao noeioethth/a aeiy oeueiai aioeieneaeaioa i?iyaey?oueny neeueiioa,
a aeiaiacii aeith/eo eiioeaio?aoeie ?icoe?th?oueny (oaae.4). Oae,
caieueoaiiy iane aenieaioia N ia na?aaeiaeuao c anei?aiiiaith eeneioith
niinoa?iaaeinue oieueee i?e 20iN, a o aenieaioia A i?e 15iN
iaeaieueoith aaee/eiith ia?aaeuaiiy eiio?ieth oa?aeoa?ecoaaeany
iaeneiaeueia aeica oei?? ?a/iaeie (1000 ia/e), yea o cae/aeieo oiiaao
cia/ii iiaiaoaaea ?ino.

Ii?iaiyiiy aeaio oeiia aenieaioia iieacaei, ui a iioeiaeueieo oiiaao N
ia?aaeuothoue A ii aiiiao?e/iei iieacieeai. A oie aea /an, N aoee cia/ii
/ooeeaioeie aei cieaeaiiy oaiia?aoo?e. Iiceoeaiee aoaeo anei?aiiiai?
eeneioe, ye i iioeo aioeieneaeaioia, i?iyaeyany neeueiioa o aenieaioia
A. Oaei aiaeiiiiinoi iiaeooue aooe iia’ycaii c inioth/eie cia/ieie
ii?oi-oiciieiai/ieie iniaeeainoyie aiiieioeeae oa nii’yaeieae.

Oaaeeoey 4

Iana eaeonia oiiaoa (a), iaea?aeaieo ia na?aaeiaeuao

c anei?aiiiaith eeneioith i?e ii?iaeueiie oa cieaeaiie

oaiia?aoo?i eoeueoeaoaaiiy (Om)

ti,Eiioeaio?aoeiy anei?aiiiai? eeneioe, ia/eN0110100N I I’ss Ae I E
I252,22±0,332,00±0,171,90±0,142,30±0,29201,19±0,081,28±0,081,33±0,061,57
±0,11**150,16±0,010,18±0,010,13±0,01*0,17±0,01A I I I E I O E E
I251,17±0,151,40±0,161,94±0,29*1,50±0,21201,79±0,021,82±0,072,10±0,102,3
2±0,14**150,35±0,020,42±0,02*0,38±0,030,38±0,03

I?eiioea. ?icieoey iiae aeineiaeiei oa eiio?ieueiei aa?iaioaie
aeinoiai?ia i?e: *?=0,95; **?=0,99.

A E N I I A E E

1. Iieacaia caeaoiinoue neioaoe/iiai aioeieneaeaioa oaiienaia
noeioethaaoe ?ino iie?iaeeaoeia oiiaoa. Iioeiaeueieie aoee eiai
eiioeaio?aoei? 10-15 ia/e. Iiaeiaiith aei?th oa?aeoa?ecoaaany
-oieioa?ie, yeee oaeiae ? inoeiiei aioeieneaeaioii-iiaiaioi?ii.
O?aaeeoeieii aeei?enoiaaii o neeaaei aeeaeeueieo na?aaeiaeu aiaeiiaieee:
anei?aiiiaa eeneioa oa aeooaoiii-SH, — ieacaeeny a oeueiio aeiaaeeo
cia/ii iaio aoaeoeaieie.

2. Aeineiaeaeaiiy aeeiaiiee ?inoo iaaiiia oa ei?aiia o iie?iaeeaoeia
iieacaei, ui noeioethth/a aeiy oaiienaia, a ia?oo /a?ao, iia’ycaia c
iioaineoieaoei?th ei?aiaooai?aiiy ia ii/aoeo eoeueoeaoaaiiy. Aeey
i?iyaeaiiy oeueiai aoaeoo aeniiceoeiy aenieaioia ia na?aaeiaeui c
aioeieneaeaioii iiaeiia noaiiaeoe ia iaio, iiae 5 aeia.

3. Aeiy oaiienaia ia ?ino aenieaioia oa iioaeoieo ?ineei (anaioe/ieo
i?i?inoeia oiiaoa) iiaeiaia. Iioeiaeueii eiioeaio?aoei? aioeieneaeaioa
a na?aaeiaeui aeey i?i?inoeia noaiiaeyoue 10-20 ia/e.

4. Caeaoiinoue oaiienaia cia/ii iiaeaeuoaaoe eieueeinoue i?eaeaoeiaeo
ei?aiia ia ?ineeii ia caeaaeeoue aiae iayaiinoi o aeeaeeueiiio
na?aaeiaeui iiiia Fe2+ oa Na2AAeOA.

5. Anoaiiaeaia inioaaiiy nooo?aeo aiaeiiiiinoae a ?aaeoei? ia aeiaeaiiy
aei aeeaeeueiiai na?aaeiaeua oaiienaia aiieaeueieo iie?iaeeaoeia ?icieo
ni?oia oiiaoa. Iaeaieueoee iiceoeaiee aoaeo aioeieneaeaioa niinoa?iaaany
aeey Iiai/ea, NO-1, NO-2.

6. A eoeueoo?i eaeonia oiiaoa ?icieoey iiae aiieiai/iith aeoeaiinoth
aioeieneaeaioia-iiaiaioi?ia oa aiaeiiaieeia aoea cia/ii neaaoith.
Aenieaioe aeiieioeeia oiiaoa aeyaeeenue aieueo /ooeeaeie aei aaaaeaiiy
aioeieneaeaioia aei na?aaeiaeua (ca aeiyoeii -oieioa?iea), iiae
aenieaioe nii’yaeieae. Noeioethth/ee aoaeo anei?aiiiai? eeneioe a
eoeueoo?i eaeonia oiiaoa cia/ii iineethaaany i?e cieaeaiii oaiia?aoo?e
eoeueoeaoaaiiy.

7. Aeyaeaii ?ino?aaoethth/i aeanoeainoi neioaoe/iiai aioeieneaeaioa
oaiienaia a eoeueoo?i oeaiei oiiaoa in vitro aeicaieythoue cai?iiiioaaoe
aeei?enoiaoaaoe eiai a neeaaei aeeaeeueieo na?aaeiaeu aeey ae?iuoaaiiy
iie?iaeeaoeia, a oiio /enei, ?acii c aoeneiaie.

8. Aeei?enoaiiy aioeieneaeaioia aeey noeioeyoei? i?ioeania ?aaaia?aoei?
a eaeonieo eoeueoo?ao iiaea aooe aoaeoeaiei oieueee iiney aeiai?o
eiie?aoii? ?a/iaeie oa ?? eiioeaio?aoei? c o?aooaaiiyi iniaeeainoae oeio
aenieaioia oa iaoe ?o eoeueoeaoaaiiy.

NIENIE IIOAEIEIAAIEO ?IAIO CA OAIITH AeENA?OAOeI?

1. Ai?aaoaiei E.TH., Nooeiaa C.A., Iieauoe N.A., Ai?aaoaiei A.E., Cic
I.I., Na?aa?yiue A.I., Noiey?iaa E.A.,Eociaoeia A.A., A?oia A.A.
Eniieueciaaiea neioaoe/aneeo aioeieneaeaioia a ea/anoaa ?aaoeyoi?ia
?inoa a eoeueoo?a oeaiae in vitro // Aeieeaaeu ?ANOI. — 1993. — N 2. —
C. 9-13.

2. Ai?aaoaiei E.TH., Cic I.I, Eoeeaiei A.A., Iieauoe N.A., Nooeiaa C.A.,
Na?iaa ?.ss. Eniieueciaaiea iee?iaeic aeieiae/anee aeoeaiuo aauanoa a
ieoaoaeueiuo n?aaeao in vitro // Aeieeaaeu ?ANOI. — 1994. — N 5. — C.
13-14.

3. Iieauoe N.A., Ai?aaoaiei E.TH. Aeeyiea neioaoe/aneiai aioeieneaeaioa
oaiienaia ia i?ioeannu ?aaaia?aoeee e iioiaaiac oiiaoa in vitro //
Aeieeaaeu ?ANOI. — 1995. — N 3. — C. 9-11.

4. Poleshchuk S., Gorbatenko I. In vitro necrosis problem under using of
a synthetic antioxidant phenoxan in tomato tissue culture// Proceedings
of the Intern. Reg. Seminar «Environment Protection: Modern Studies In
Ecology And Microbiology».- Uzhgorod, 1997. — V.1 -P.325-328.

5. Ai?aaoaiei E.TH., Nooeiaa C.A., Iieauoe N.A., Ai?aaoaiei A.E., Cic
I.I., Na?aa?yiue A.I., Noiey?iaa E.A.,Eociaoeia A.A., A?oia A.A.
?aaoeyoi?u ?inoa a eoeueoo?a oeaiae in vitro ec eeanna
aeiaioeieneaeaioia// Aoi?ay eiioa?aioeey «?aaoeyoi?u ?inoa e ?acaeoey
?anoaiee». Oac. aeiee. (29 ethiy — 1 ethey 1993 a.). — I. 1993. — *.2. —
C. 89-90.

6. Poleshchuk S.V., Gorbatenko I.Yu. In vitro ascorbic acid influence on
callus formation and plant regeneration // Proceedings of the Latvian
Academy of Sciences. — 1995. — N 5/6. — P. A35.

7. Iieauoe N.A., Ai?aaoaiei E.TH. Eco/aiea caaeneiinoe aeaenoaey
oaiienaia ia ?ino aiaenia oiiaoa in vitro io niaea?aeaiey eiiia Fe2+ e
Na2YAeOA a ieoaoaeueiie n?aaea // Oac. eiio. «Aeooaeueiua i?iaeaiu
aeioaoiieiaee a ?anoaieaaiaenoaa, aeeaioiiaiaenoaa e aaoa?eia?ee». — I.,
1996. — C. 65.

8. Iieauoe N.A., Ai?aaoaiei E.TH. Aeaenoaea aeooaoeiia ia
eaeeoniia?aciaaiea e ?aaaia?aoeeth oiiaoa // Oac. eiio. «Aeooaeueiua
i?iaeaiu aeioaoiieiaee a ?anoaieaaiaenoaa, aeeaioiiaiaenoaa e
aaoa?eia?ee». — I.,1996. — C. 66.

Iieauoe N.A. Aiieiai/ia aeoeaiinoue aioeieneaeaioia a eoeueoo?i oeaiei
oiiaoa in vitro. — ?oeiien.

Aeena?oaoeiy ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa aiieiai/ieo iaoe
ca niaoeiaeueiinoth 03.00.20 — aiioaoiieiaiy. — Oa?niinueeee aea?aeaaiee
iaaeaaiai/iee oiiaa?neoao Iiiinoa?noaa inaioe Oe?a?ie, Oa?nii, 1998.

Aeena?oaoeith i?enay/aii i?iaeaii aieeao aioeieneaeaioia (AI) ia ?ino oa
?icaeoie aenieaioia oiiaoa in vitro. A ?acoeueoaoi i?iaaaeaieo
aeineiaeaeaiue iieacaii, ui AI caeaoii ?aaoethaaoe ?ino ei?aiia,
iaaiiia, ?aaaia?aoeith c eaeonia i oiio iiaeooue aeei?enoiaoaaoeny o
neeaaei aeeaeeueieo na?aaeiaeu ia oieueee ye aioeno?aniai ?a/iaeie, a e
o yeinoi noeioeyoi?ia ?inoo. Anoaiiaeaia iayaiinoue inoioieo
iniaeeainoae aiieiai/ii? aeoeaiinoi AI, ?icieo ca iaoaiiciii
aioeieenethaaeueii? aei? (AI-iiaiaioi?e oa ?a/iaeie-aiaeiiaieee).
Aeineiaeaeaii iniaeeainoi ?aaeoei? ia aeiaeaiiy AI aei na?aaeiaeua
?icieo oeiia aenieaioia. Iaeaieueo ia?niaeoeaiei noeioeyoi?ii ?inoo aeey
iie?iaeeaoeia oiiaoa aeyaeany neioaoe/iee AI oaiieueii? i?e?iae?
oaiienai, aeei?enoaiee a eoeueoo?i ?ineeiieo oeaiei in vitro aia?oa.
Eiai canoinoaaiiy ia iniiai aeiai?o iioeiaeueieo aeey eiie?aoieo aeaeia
oa ni?oia eiioeaio?aoeie iiaea aooe ?aeiiaiaeiaaia aeey iiaeaeuaiiy
aoaeoeaiinoi oaoiieiaie iie?i?iciiiaeaiiy in vitro.

Eeth/iai neiaa: aioeieneaeaio, oaiienai, eoeueoo?a oeaiei, noeioeyoi?
?inoo, aenieaio, oiiao.

Iieauoe N.A. Aeieiae/aneay aeoeaiinoue aioeieneaeaioia a eoeueoo?a oeaie
oiiaoa in vitro. — ?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe
ii niaoeeaeueiinoe 03.00.20 — aeioaoiieiaey. — Oa?niineee
ainoaea?noaaiiue iaaeaaiae/aneee oieaa?neoao Ieienoa?noaa ia?aciaaiey
Oe?aeiu, Oa?nii, 1998.

Aeenna?oaoeey iinayuaia i?iaeaia aeeyiey aioeieneaeaioia (AI) ia ?ino e
?acaeoea yenieaioia oiiaoa in vitro. A ?acoeueoaoa i?iaaaeaiiuo
enneaaeiaaiee iieacaii, /oi AI niiniaiu ?aaoee?iaaoue ?ino ei?iae,
iiaaaia, ?aaaia?aoeeth ec eaeeonia e iiyoiio iiaoo eniieueciaaoueny a
ieoaoaeueiuo n?aaeao ia oieueei eae aioeno?anniaua aauanoaa, ii e eae
noeioeyoi?u ?inoa. Onoaiiaeaii iaee/ea nouanoaaiiuo iniaaiiinoae
aeieiae/aneie aeoeaiinoe AI, ?acee/athueony ii iaoaiecio
aioeieeneeoaeueiiai aeaenoaey (AI-eiaeae-oi?u e
aauanoaa-ainnoaiiaeoaee). Eco/aiu iniaaiiinoe ?aaeoeee ia aeiaaaeaiea AI
a n?aaeo ?acee/iuo oeiia yenieaioia. Iaeaieaa ia?niaeoeaiui noeioeyoi?ii
?inoa aeey iee?i/a?aieia oiiaoa ieacaeny neioaoe/aneee AI oaiieueiie
i?e?iaeu oaiienai, eniieueciaaiiue a eoeueoo?a ?anoeoaeueiuo oeaiae in
vitro aia?aua. Aai i?eiaiaiea, iniiaaiiia ia iiaeai?a iioeiaeueiuo aeey
eiie?aoiuo aeaeia e ni?oia eiioeaio?aoeee, iiaeao auoue ?aeiiaiaeiaaii
aeey iiauoaiey yooaeoeaiinoe oaoiieiaee iee?i?aciiiaeaiey in vitro.

Eeth/aaua neiaa: aioeieneaeaio, oaiienai, eoeueoo?a oeaiae, noeioeyoi?
?inoa, yenieaio, oiiao.

Poleshchuk S.V. Biological activity of antioxidants in in vitro tomato
tissue culture. — Manuscript.

Thesis for a PhD’s degree by speciality 03.00.20 — biotechnology. —
Kherson State Pedagogical University of Ministry of Education of
Ukraine, Kherson, 1998.

The dissertation is devoted to the problem of in vitro antioxidants (AO)
effect on tomato explants growth and development. Our results showed,
that AO regulate growth of roots, shoots, regeneration from callus and
can play an active role in nutritious media not only as antistress
agents, but as growth stimulators too. We determined, that AO-inhibitors
and reducing agents have considerable differences in biological
activity. There were studied particularities of different explant types
reaction on addition AO into medium. Phenoxan, a phenolic synthetic AO,
is the most perspective for tomato micropropagation. It using, based on
determination of optimal concentrations for concrete cultivars, may
increase considerably efficiency of technologies of in vitro
micropropagation.

Key words: antioxidant, phenoxan, tissue culture, growth stimulator,
explant, tomato.

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