Вплив катіонів лужноземельних і перехідних металів на функціонування мітохондрій печінки щурів: Автореф. дис… канд. біол. наук / Л.С. Вовканич, Київ

4

EE?ANUeEEE OI?AA?NEOAO ?iai? OA?ANA OAA*AIEA

AIAEAIE* ETHAIIE? NOAIAIIAE*

OAeE 612.014.46:577.352.4

AIEEA EAO?II?A EOAEIICAIAEUeIEO ? IA?AO?AeIEO IAOAE?A IA
OOIEOe?IIOAAIIss I?OIOIIAe??E

IA*?IEE UO??A

03.00.02 — a?io?ceea

Aaoi?aoa?ao aeena?oaoei? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa aiieiai/ieo iaoe

Ee?a-1999

Aeena?oaoei?th ? ?oeiien.

?iaioa aeeiiaia o Eueaianueeiio aea?aeaaiiio oiiaa?neoaoi

iiaii ?aaia O?aiea Iiiinoa?noaa Inaioe Oe?a?ie.

Iaoeiaee ea?iaiee: eaiaeeaeao aiieiai/ieo iaoe, aeioeaio

AeOAEOeUeEEE Eaiiiae
Ieaenaiae?iae/

Eueaianueeee aea?aeaaiee
oiiaa?neoao iiaii ?aaia O?aiea,

aeioeaio eaoaae?e oiciieiai?
ethaeeie i oaa?ei

Ioioeieii iiiiaioe: aeieoi? a?ieia?/ieo iaoe, no. iaoe. ni?a?ia?oiee

EINOA??I Na?a?e Ieaen?eiae/

caa. a?aeae?eo ?inoeoooo a?io?i??

?i. I.A.Iaeeaae?ia IAI Oe?a?ie

aeieoi? a?ieia?/ieo iaoe, i?ioani?

AA?ACIANUeEEE Aaaeei sseeiiae/

caa. a?aeae?eo ?inoeoooo o?c?ieia??

?i. I.I.Aiaiiieueoey IAI Oe?a?ie

I?iaiaeia onoaiiaa: Iaoe?iiaeueiee iaaee/iee oi?aa?neoao ?iai?
I.I.Aiaiiieueoey IIC Oe?a?ie, i. Ee?a

Caoeno aiaeaoaeaoueny «22» aa?niy 1999 ?. i 16 aiae. ia caniaeaiii
niaoeiaeiciaaii? a/aii? ?aaee Ae 26.001.38 i?e Ee?anueeiio
oi?aa?neoao? ?iai? Oa?ana Oaa/aiea ca aae?anith: 252033, i. Ee?a, aoe.
Aieiaeeie?nueea 64, a?ieia?/iee oaeoeueoao Ee?anueeiai oi?aa?neoaoo
?iai? Oa?ana Oaa/aiea, e?iiaoa 215.

C aeena?oaoei?th iiaeia iciaeiieoenue o aiaeiioaoei Ee?anueeiai
oi?aa?neoaoo ?iai? Oa?ana Oaa/aiea ca aae?anith: 252033, i. Ee?a, aoe
Aieiaeeie?nueea, 58.

Aaoi?aoa?ao ?icineaiee «21» na?iiy 1999 ?.

A/aiee nae?aoa? niaoeiaeiciaaii?

a/aii? ?aaee, eaiaeeaeao a?ieia?/ieo iaoe
O.E.Aeaaeaeianueea

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeueiinoue oaie. Aeineiaeaeaiiy caeiiiii?iinoae ooieoeiiioaaiiy
eeioei i?aaiicio a aeno?aiaeueieo oiiaao, o oiio /enei iiae aieeaii
aio?iiiaaiieo oaeoi?ia, ? iaeiei ic i?ii?eoaoieo iai?yieia no/anii?
aiieiai?. Na?aae oaeeo oaeoi?ia iaeia c ia?oeo iinoeue caeiathoue
eao?iie iaoaeia, yei oa?aeoa?ecothoueny oe?ieei niaeo?ii iaoiaaiiiai
aieeao ia i?aaiici ethaeeie i oaa?ei. Aiie aieeaathoue ia aeoeaiinoue
?yaeo oa?iaioia (Eaaeia, 1972, A?oia, Ieaoiaaa, 1989), noai eeioeiieo
iaia?ai (Oeeueyin, 1978, Venugopal, Luckey, 1978, Evans, 1990),
aenooiathoue aioaaiiinoaie eaeueoeith a eeioeii (Eeaaaoeue, Iaiueei,
1992, Aeoaeoeueeee, Naaaaeao, 1995, Iiiiia?aa, 1980). Oea nooo?ai
aiaeaeaa?oueny ia ooieoeiiiaeueiie aeoeaiinoi eeioei oa ?o i?aaii?aeia,
cie?aia iioioiiae?ie (IO), yei caeaoii iaeiie/oaaoe ?yae iaoaeia i?e
iioieneeaoei? ieie a oiiaao in vivo (Probst, 1979, Korck, 1986).
Ii?ooaiiy ooieoeiiioaaiiy IO, yei caeiathoue aaaeeeaa iinoea o
caaacia/aiii aia?aaoe/ieo iio?aa (Gunter et. al., 1994) i eaeueoei?aiai
aiiainoaco (Gunter, Pfeiffer, 1990) eeioei, iiaea aooe iaeiei c
iaoaiiciia, ca yeei ?aaeico?oueny oiene/iee aoaeo iaoaeia. I?ioa
aieueoinoue aeineiaeaeaiue aieeao eaoiiiia iaoaeia ia ooieoeiiioaaiiy IO
iinyoue o?aaiaioa?iee oa?aeoa? i a iniiaiiio iia’ycaii ic aea/aiiyi
eaeueoeieo?ainii?oieo ooieoeie oeeo i?aaiae. I?aeoe/ii aiaenooii ?iaioe,
i?enay/aii eiiieaeniie ioeiioei ii?ooaiue iniiaieo ooieoeie IO iiae
aieeaii eaoiiiia iaoaeia. Neaai aeineiaeaeaiei caeeoa?oueny oaeiae
ieoaiiy caeaaeiinoi aoaeoia iaoaeia ia ooieoe?? IO aiae ?o
oiceei-oiii/ieo aeanoeainoae, cie?aia aiae nii?iaeiaiinoi aei ?icieo
aiieiaaiaeia. Aiaenooiinoue aeinoaoiuei? aace aeaieo o oeueiio iai?yieo
iaiaaeo? iiaeeeaino? ?ic?iaee iaoiaeia iiaeaeuaiiy noieeinoi i?aaiiciia
aei oeeo oaeoi?ia, oa iaoiaeia oa?aii? iioieneeaoeie, aeeeeeaieo nieyie
iaoaeia.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. Aeaia ?iaioa
aeeiiaia a ?aieao iaoeiai-aeineiaeieo oai «Aea/aiiy eeioeiieo iaoaiiciia
ii?ooaiue nae?aoi?ii? ooieoei? oeoieiaeo caeic eaoiiiaie iaoaeia» (?
aea?ae?a?no?aoei? 0194 V 015656), «Aeineiaeaeaiiy iaoaiiciia aei?
eaoiiiia ia?aoiaeieo iaoaeia ia nae?aoi?io ooieoeith eeioei o?aaieo
caeic» (? aea?ae?a?no?aoei 0195 V 006596), «Aeyniaiiy iaoaiiciia
ooieoeiiioaaiiy Na-Ca iaiiio iaa?aie nae?aoi?ieo eeioei» (?
aea?ae?a?no?aoei? 1096 V 002178) oa Aeine?aeaeaiiy Na-caeaaeiiai
o?ainii?oo eaeueoe?th /a?ac iaia?aie nae?aoi?ieo ee?oei o i?yiiio ?
cai?ioiiio iai?yiao oa ?ie? o iueiio ooieoe?iiaeueieo a?oi i?ioa?io
iai?iieea (? aea?ae?a?no?aoe?? 0198 V 004853).

Iaoa i caaea/? aeineiaeaeaiiy. Aea/eoe ia?aeii? iaoaiicie aei? eaoiiiia
iaoaeia c ?icieie oiceei-oiii/ieie aeanoeainoyie (Ca2+, Sr2+, Mn2+,
Cd2+, La3+) ia ooieoeiiioaaiiy IO ia/iiee uo?ia. Oey iaoa aeinyaaeanue
ae?ioaiiyi oaeeo iniiaieo caaaeaiue: 1) c’ynoaaoe iaoaiicie aieeao
eaoiiiia iaoaeia ia aeeoaiiy IO oa o?aineieaoeith i?ioiiia /a?ac
iaia?aio oeeo i?aaiae; 2) aeineiaeeoe aieea iaoaeia ia
i?ioiiioi?-noeioeueiaaia aeeoaiiy IO oa oinoi?eethaaiiy ADP oeeie
i?aaiaeaie; 3) aea/eoe iaoaiicie aieeao eaoiiiia iaoaeia ia
Na2+-aeoioethth/o caeaoiinoue IO; 4) aea/eoe aieea eaoiiiia iaoaeia ia
noai i?iieeiinoi aioo?ioiuei? iaia?aie IO ia/iiee oa iaoaiicie oeueiai
aieeao; 5) c’ynoaaoe caeaaeiinoue aoaeoia eaoiiiia iaoaeia ia
ooieoeiiioaaiiy IO aiae ?o oiceei-oiii/ieo aeanoeainoae.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoaoia. Aia?oa i?iaaaeaii eiiieaenia
aeineiaeaeaiiy ciii ooieoeiiiaeueiiai noaio IO ia/iiee iiae aieeaii
eaoiiiia ?yaeo iaoaeia c ?icieie oiceei-oiii/ieie aeanoeainoyie (Ca2+,
Sr2+, Mn2+, Cd2+, La3+). O i?ioeani aeineiaeaeaiue io?eiaii oaei iiai
aeaii: 1) caeaoiinoue eaoiiiia iaoaeia noeioethaaoe aeeoaiiy IO
iaoiiaeaia ?o aia?aicaeaaeiei o?ainii?oii o iao?een oeeo i?aaiae i
oa?aeoa?eco?oueny iaa?iaiith ei?aeyoe??th c aioaeueii?th ?o aiae?aoaoei?
oa nii?iaeiaiinoth aei ea?aieneeueieo a?oi Na2+-oiiii?oa?a IO; 2) aieea
eaoiiiia ?icieo a?oi iaoaeia ia aeeoaiiy IO ia caeaaeeoue aiae noano?aoo
ieeneaiiy; 3) caeaoiinoue eaoiiiia iaoaeia i?eaii/oaaoe
i?ioiiioi?-noeioeueiaaia aeeoaiiy IO oa oinoi?eethaaiiy ADP oeeie
i?aaiaeaie ei?aeth? c ?o nii?iaeiaiinoth aei SH-a?oi a?ie?aaiae?a; 4)
eaoiiie iaoae?a c aenieith nii?iaeiaiinoth aei eeniaaiinieo eiaaiaeia
(Mn2+ i ii.) iiaiaothoue o?ainii?o Na2+ o IO ca eiieo?aioiei oeiii, a
eaoiiie iaoae?a c aenieith nii?iaeiaiinoth aei ni?eiaiinieo eiaaiaeia
(Cd2+) — ca ciioaiei oeiii; 5) caeaoiinoue eaoiiiia iaoaeia iiaiaoaaoe
o?ainii?o Na2+ a IO oa?aeoa?eco?oueny iaa?iaiith ei?aeyoe??th c
aioaeueii?th ?o aiae?aoaoei? oa nii?iaeiaiinoth aei ea?aieneeueieo a?oi
Na2+-oiiii?oa?a; 6) eaoiiie Na2+ i Cd2+ iiaeoeothoue ia?aoiae
aioo?ioiuei? iaia?aie IO o noai aeniei? ianiaoeeoi/ii? i?iieeiinoi ca
?icieie iaoaiiciaie, i?ioa oeyoii oi?ioaaiiy iaeieo i oeo aea ii?; 7)
eaoiiie Sr2+ i Mn2+ i?eaii/othoue Na2+-iiaeoeiaaii ciiie i?iieeiinoi
aioo?ioiuei? iaia?aie IO ca eiieo?aioiei oeiii.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoaoia. Io?eiaii ?acoeueoaoe
iiaeeaeththoue i ooi/iththoue ciaiiy i?i iaoaiicie aieeao eao?ii?a
iaoaeia ic ?icieie oiceei-oiii/ieie aeanoeainoyie ia ooieoeiiioaaiiy IO.
Aiie ?icoe?ththoue aaco aeaieo, iaiaoiaeieo aeey ?ic?iaee iaoiaeia
iiaeaeuaiiy noieeinoi i?aaiiciia aei iioieneeaoeie eaoiiiaie iaoaeia, a
oaeiae aeey iai?aaeaiiai iiooeo aioeaeio?a aei eao?ii?a iaoae?a c
??ciith nii??aeiai?noth aei eenia- ? n??eiai?nieo a?oi e?aaiae?a
ee?oeie, ui ia? aaaeeeaa cia/aiiy aeey Oe?a?ie o ca’yceo c aenieei
oaoiiaaiiei caa?oaeiaiiyi ?yaeo ?? ?aaiiiia. ?acoeueoaoe
aeei?enoiaothoueny i?e aeeeaaeaiii caaaeueiiai eo?no «Oiciieiaiy
ethaeeie i oaa?ei», oa niaoeeo?nia «Oiciieiaiy iiia?aeueiiai iaiiio» i
«Aiiaia?aaoe/ii iniiae oiciieiai/ieo i?ioeania» o Eueaianueeiio
aea?aeaaiiio oiiaa?neoaoi iiaii ?aaia O?aiea, a oaeiae caaaeueiiai eo?no
«Ii?iaeueia oiciieiaiy» o Eueaianueeiio aea?aeaaiiio iaaee/iiio
oiiaa?neoaoi. Aiie iiaeooue aooe aeei?enoaii aeey iiaeaioiaee
niaoeiaeinoia iaaeeei-aiieiai/iiai i?ioieth a iioeo o/aiaeo caeeaaeao
Oe?a?ie.

Iniaenoee aianie caeiaoaa/a iieyaa? o aeeiiaiii anueiai ianyao
aenia?eiaioaeueii? /anoeie aeena?oaoei?, noaoenoe/iie ia?iaoei
?acoeueoaoia, iiaeai?i i ii?aoethaaiii aeaieo eioa?aoo?e, a oaeiae, ca
o/anoth iaoeiaiai ea?iaieea oa ni?aaaoi??a, o aiaeici oa iioa?i?aoaoei?
iaea?aeaieo ?acoeueoaoia.

Ai?iaaoeiy ?acoeueoaoia aeena?oaoe??. ?acoeueoaoe aeineiaeaeaiue, ui
aeeth/aii aei aeena?oaoei?, aeiiiaiaeaeenue ia: 1-io c’?caei
Oe?a?inueeiai aiioice/iiai oiaa?enoaa (1994 ?., Ee?a), XIV c’?caei
Oe?a?inueeiai oiciieiai/iiai oiaa?enoaa ii. ?.I. Iaaeiaa (1994 ?.,
Ee?a), iaoeiai-i?aeoe/iiio neiiicioii «Iaaeeei-aiieiai/ii i?iaeaie
aaeaioaoei? a no/anieo oiiaao inioaaiiy i?aaiicio» (1995 ?.,
Eociaoeianuee), eiioa?aioei? aei 100-?i//y eaoaae?e oiciieiai?
Eueaianueeiai aea?aeaaiiai iaaee/iiai oiiaa?neoaoo (1995 ?., Eueaia),
ui?i/ieo iaoeiaeo eiioa?aioeiyo Eueaianueeiai oiiaa?neoaoo (1994-1998
?., Eueaia), 9-oie ?a?iiaenueeie eiioa?aioei? c i?iaeai aiiaia?aaoeee
(1996 ?., Eiaaei-ea-Iaoa), 4-ie ?a?iiaenueeie nooaeaionueeie iaoeiaie
eiioa?aioei? (1996 ?., Aaeainuee), VII Oe?a?inueeiio aiioiii/iiio
c’?caei (1997 ?., Ee?a), XV c’?caei Oe?a?inueeiai oiciieiai/iiai
oiaa?enoaa (Aeiiaoeuee, 1998 ?.).

Ioae?eaoe??. Iniiaii iieiaeaiiy aeena?oaoei? aenaioeaii o 11
ioaeieaoeiyo, o oiio /enei o 3 noaooyo a iaoeiaeo aeo?iaeao oa 8 oacao.

No?oeoo?a aeena?oaoei?. Aeena?oaoeiy neeaaea?oueny c anooio, iaeyaeo
eioa?aoo?e, iieno ia’?eoa i iaoiaeia aeineiaeaeaiue, aeeeaaeo io?eiaieo
?acoeueoaoia oa ?o iaaiai?aiiy, aeniiaeia, nieneo aeei?enoaieo aeaea?ae
(332 iacae) ? aeiaeaoe?a. ?iaioa aeeeaaeaia ia 106 noi?iieao iniiaiiai
oaenoo, iinoeoue 10 oaaeeoeue, 14 ?enoie?a oa aeiaeaoee ia aeaio
noi??ieao.

INIIAIEE CI?NO

IA’?EO ? IAOIAeE AeINE?AeAEAIUe

Aeineiaeaeaiiy iaoaiiciia aei? eaoiiiia iaoaeia ia ooieoeiiioaaiiy IO
i?iaiaeeee a oiiaao in vitro. Aeei?enoiaoaaee oei?eaee iaoaeia. IO
iciethaaee c ia/iiee aieeo uo?ia iaoiaeii aeeoa?aioeiaeueiiai
oeaio?eooaoaaiiy (Eiiae?aoiaa, A?eai?aiei, 1985). Aei neeaaeo
na?aaeiaeua aeaeieaiiy IO aoiaeeee (iiieue/e): naoa?ica — 300, o?in — 10
(?I 7,4, t 0oC). Aeeoaiiy IO aea/aee iiey?ia?aoi/iei iaoiaeii c
aeei?enoaiiyi cae?eoiai aeaeo?iaea Eea?ea oa eeniaaeii?thaaeueiiai aeieo
AO-012 (IAe? aiie. aeineiaeaeaiue, Ioueii, ?iniy). Na?aaeiaeua
iieoaaoei? IO iinoeei (iiieue/e): naoa?ica — 150, KCl — 50, KH2PO4 —
1,

o?in — 5, noeoeeiao — 0,35 (?I 7,4, t 26oC). Aeoiae I+ c IO ia/iiee
?a?no?oaaee ?I-iao?e/iei iaoiaeii c aeei?enoaiiyi aeaeo?iaea YNE-43-07
oa oiiaa?naeueiiai iiiiiao?a YA-74. O oeeo aeineiaeaeaiiyo
eiioeaio?aoeith o?ino o na?aaeiaeui

iieoaaoei? ciaiooaaee aei 3 iiieue/e (?I 7,4, t 26oC).

Eaeueoeieaeoioethth/o ooieoeith IO aea/aee c aeei?enoaiiyi
?aaeiiaeoeaiiai icioiio 45Ca2+ (0,37 IAe/iiieue) o na?aaeiaeui
iieoaaoei? ic ciaioaiith eieueeinoth KH2PO4 (0,1 iiieue/e). Iiaeeiaiiy
45Na2+ coieiyee aianaiiyi o noniaicith IO 25 iee noii-?ic/eio (naoa?ica
— 150 iiieue/e, KCl — 50 iiieue/e, o?in — 5 iiieue/e, LaCl3 — 20
iiieue/e, ?I 7,4) ic ianooiiei ?? oaeaeeei oieueo?oaaiiyi /a?ac
iaia?aiii oieueo?e Synpor (0,45 iei). Oieueo?e i?iieaaee 5 ie
na?aaeiaeua oaeiai neeaaeo (iiieue/e): KCl — 150, o?in — 5, LaCl3 — 0,5
(?I 7,4). ?aaeiiaeoeaiinue oieueo?ia aeii?thaaee ia ?iaeeiiiio
noeeioeeyoeieiiio ei/eeueieeo Rackbeta-1219 c aeei?enoaiiyi
noeeioeeyoeieii? ?iaeeie AEN-8.

Ianiaoeeoi/io i?iieeiinoue iioioiiae?iaeueii? iaia?aie ioeiithaaee ca
iaaooaiiyi IO iaoiaeii ?a?no?aoei? naioeiiiaeeiaiiy noniaici? oeeo
i?aaiae (? = 520 ii) ia niaeo?ioioiiao?i Cary-210. O na?aaeiaeua
aeaeieaiiy IO aeiaeaoeiai aiineee AAOA (1 iiieue/e) i aeueaoiii
ne?iaaoee aeea (1%, iana/ia’?i). Na?aaeiaeua iieoaaoei? IO iinoeei
(iiieue/e): naoa?ica — 150, KCl — 50, KH2PO4 — 2, o?in — 5, noeoeeiao —
1 (pH 7,4, t 30iN). Aiino aieea a noniaici? IO aecia/aee ca iaoiaeii
Eio?i (Lowry et. al., 1951) aai aio?aoiaei iaoiaeii (Bialey, 1967).

Io?eiaii ?acoeueoaoe ia?iaeyee noaoenoe/ii c aeei?enoaiiyi e?eoa?ith
Nouethaeaioa, a oaeiae iaoiaeaie ei?aeyoeieiiai oa ?aa?anieiiai aiaeico.

?ACOEUeOAOE AeINE?AeAEAIUe

Aieea eao?ii?a eoaeiicaiaeueieo i ia?aoiaeieo iaoaeia ia aeeoaiiy
iioioiiae?ie oa o?aineieaoe?th i?ioii?a /a?ac i?oioiiae??aeueio
iaia?aio.

A?aeiii (Saris, Akerman, 1980), ui eao?iie Na2+, Sr2+, Ba2+ oa Mn2+
aeeeeeathoue noeioeyoeith aeeoaiiy icieueiaaieo IO. Aeyaeaiee
iaie aiia?aiei/iee oa?aeoa? (?en. 1) aeiciai? caeaaeiinoi aoaeoia Na2+,
Sr2+, Mn2+ oa Cd2+ (50-400 ieiieue/e) ia aeeoaiiy IO ia/?iee aeicaieea
aecia/eoe eiinoaioo iaiiaiane/aiiy (K0,5) oa iaeneiaeueio oaeaeeinoue
(Vmax) oeueiai i?ioeano iaoiaeii eiiaa?ecaoei? o nenoaii iaa?iaieo
eii?aeeiao Eaeioiaa?a-Aa?ea. Eaoiiie La3+ (50-400 ieiieue/e) ia aieeaaee
ia aeeoaiiy IO, a ca aiaenooiinoi o na?aaeiaeui iieoaaoei? aeciaaiieo
oinoaoia aeaui i?eaii/oaaee eiai. ?ia?a?oi?io eiinoaioo (Ki) o oeueiio
aeiaaeeo aecia/aee o eii?aeeiaoao Oaaa. Anoaiiaeaii, ui cia/aiiy Vmax
iaoae-noeioeueiaaiiai aeeoaiiy IO niaaea? o oaeiio ii?yaeeo:

Eaoiiie iaoaeia: Na2+ > Sr2+ > Cd2+ > Mn2+
>> La3+

Vmax, iiieue I2/oa•ia aieea: 35,71 33,90 26,32 22,02
9,04

K0,5 aeey aeineiaeaeoaaieo iaoaeia oa Ki aeey La3+, yei oa?aeoa?ecothoue
/ooeeainoue IO aei eaoiiiia iaoaeia ooai?ththoue oaeee ?yae:

Eaoiiie iaoaeia: Cd2+ < Ca2+ << La3+ < Sr2+ < Mn2+ K0,5, Ki (La3+), ieiieue/e: 12,63 14,82 39,79 100,68 188,30 ?en. 1. Aieea eaoiiiia iaoaeia ia oaeaeeinoue aeeoaiiy i?oioiiae??e ia/iiee (V, iiieue I2/oaia a?eea). a) Caeaaei?noue oaeaeeino? aeeoaiiy i?oioiiae??e a?ae eiioeaio?aoe?? eao?ii?a iaoae?a ([Ian+], ieiieue/e) o noniaic?? oeeo i?aaiae (ioieoe?ia eiiiy - o na?aaeiaeui iieoaaoei? aiaenooiie aeciaaiiee oinoao); a) aieea Ca2+, Sr2+, Mn2+ (100 ieiieue/e) oa Cd2+ i La3+ (50 ieiieue/e) ia oaeaee?noue aeeoaiiy iioioiiae?ie iiae /an ieeneaiiy ieie noeoeeiaoo (0,35 iiieue/e, caoo?eoiaai? noiai/eee) i ?-eaoiaeooa?aoo (1 iiieue/e, oaii? noiai/eee). Ca caeaoiinoth noeioethaaoe aeeoaiiy IO eaoiiie iaoaeia ooai?ththoue aiaeiai/iee ?yae iiae /an ieeneaiiy ieie noeoeeiaoo (0,35 iiieue/e) i ?- eaoiaeooa?aoo (1 iiieue/e) (aeea. ?en. 1). Oea naiae/eoue, ui aieea eaoiiiia iaoaeia ia aeeoaiiy IO iaei caeaaeeoue aiae noano?aoo ieeneaiiy. Aiaeiii (Nicholls, Akerman, 1982), ui aia?aicaeaaeia iiaeeiaiiy Na2+ iioioiiae?iyie noi?iaiaeaeo?oueny aeoiaeii ic ieo I+. Cie?aia, iaie aeyaeaii, ui iiaeeiaiiy 55,997,47 iiieue Na2+ IO noi?iaiaeaeo?oueny aeoiaeii c oeeo i?aaiae 49,732,44 iiieue I+. Anoaiiaeaii oaeiae, ui eaoiiie onio aeineiaeaeoaaieo iaoaeia noeioeththoue aeoiae I+ c IO ia/iiee (?en. 2), ui o aeiaaeeo Na2+, Sr2+ i Mn2+ i?aeoaa?aeaeo?oueny aeaieie ?ioeo aaoi??a (Saris, Akerman, 1980). Aeiciaa caeaaeiinoue oaeaeeinoi aeoiaeo I+ c IO aeey eaoiiiia Na2+, Sr2+ i Mn2+ (25-400 ieiieue/e) iaea aiia?aiei/iee, a aeey eaoiiiia Cd2+ i La3+ - eoiieiiiaeiaiee oa?aeoa?. A oeieiio ca caeaoiinoth noeioethaaoe aeoiae I+ c IO (Vmax, iiieue I+/oa•ia aieea) eaoiiie iaoaeia ooai?ththoue oaeee ?yae: Eaoiiie iaoaeia: Ca2+ > Sr2+ >> La3+ > Cd2+
> Mn2+

Vmax, iiieue I+/oa•ia aieea: 990,10 800,00 166,95 108,39
95,15

Io?eiaiee ?yae aiaeiai/iee ?yaeiai iaoaeia, iiaoaeiaaiiio ia iniiai ?o
caeaoiinoi aei noeioeyoei? aeeoaiiy IO, ca aeiyoeii iiiia La3+, yei o
oeueiio ?yae? ciiuothoueny aeiai.

Aeey c’ynoaaiiy ?iei o?ainii?oo eaoiiiia aeineiaeaeoaaieo iaoaeia o
iao?een

?en. 2. Aieea ?icieo eiioeaio?aoeie eaoiiiia iaoaeia ([Ian+], ieiieue/e)
ia oaeaeeinoue aeoiaeo I+ (V, iiieue I+/oa•ia aieea) c i?oioiae??e
ia/iiee.

IO o ?aaeicaoei? ?o aieeao ia ooieoeiiioaaiiy oeeo i?aaiae iaie
aoea i?iaaaeaia na?iy aeineiaeia c aeei?enoaiiyi iaeiieo?aioiiai
iiaiaioi?a Na2+-

oiiii?oa?a iioioiiae?iaeueii? iaia?aie — ?ooaiith /a?aiiiai. Iieacaii,
ui ?ooaiie /a?aiiee (10 ieiieue/e) ciaioo? noia?iee aeoiae I+ c IO
ia/iiee, yeee noeioeueiaaee eaoiiiaie Na2+, Sr2+ i Mn2+ (100 ieiieue/e),
aiaeiiaiaeii ia 97, 100 oa 90% (P< 0,05). I?ioa ?ooaiie /a?aiiee ia aieeaaa ia noia?iee aeoiae I+ c IO ia/iiee, yeee noeioethaaee eaoiiiaie Cd2+ i La3+ (50 ieiieue/e), oi/a ciaiooaaa eiai oaeaeeinoue ia 65 i 43% (P< 0,05). Oea na?ae/eoue, ui noeioeyoeiy aeeoaiiy IO oa aeoiaeo c ieo I+ eaoiiiaie Ca2+, Sr2+, Mn2+ aecia/a?oueny ?o o?ainii?oii a oei i?aaiaee ca o/anoth Na2+-oiiii?oa?a. Eoiieiiiaeiaiee oa?aeoa? aeiciai? caeaaeiinoi aoaeoia Cd2+ i La3+ ia aeoiae I+ c IO oa iaiiaia iiaiaoaaiiy oeueiai i?ioeano ?ooaii?i /a?aiiei naiae/aoue, ui a?i eeoa /anoeiai aecia/a?oueny ?o o?ainii?oii o IO /a?ac Na2+-oiiii?oa?. ?yae iaoaeia, iiaoaeiaaiee ia iniiai ?o caeaoiinoi aei noeioeyoei? aeeoaiiy IO, oa?aeoa?eco?oueny oiniith iaa?iaiith ei?aeyoei?th (oaae. 1) c aioaeue- Oaaeeoey 1 Ei?aeyoe?eiee aiae?c caeaaeiino? eiiaoe/ieo ia?aiao?ia iaoae-noeioeueiaaiiai aeeoaiiy i?oioiiae??e aiae oiceei-oiii/ieo aeanoeainoae iiiia iaoaeia. Eiaoioei?io ei?aeyoei?, rEiiaoe/ii ia?aiao?e aeeoaiiy i?oioiiae??e?ii. ?aaeion, ii?Ii aiae?, eeae/iieuelgK1 iaoae-aoeaoaolgK1 iaoae-AsplgE1 iaoae-Cys Vmax, iiieue I2/ oa•ia aieea-0,25 p=0,35-0,95 p>0,98-0,90
p=0,96-0,84 p=0,93-0,20 p=0,28I?eiioea. ? — ai?iaiaeiinoue eiaoioei?ioa
ei?aeyoei?.

ii?th aiae?aoaoei? eaoiiiia iaoaeia (r = -0,95), oa eiinoaioaie
noieeinoi ?o eiiieaenia c aoeaoaoii (r = -0,90) oa ania?aaiiiaith
eeneioith (r = -0,84). Oea naiae/eoue i?i aaaeeeao ?ieue
aeaaiae?aoaoei? eaoiiiia iaoaeia oa ?o aca?iiaei? c
ea?aieneeueieie a?oiaie Na2+-oiiii?oa?a iiae /an ?o aia?aicaeaaeii?
o?aineieaoei? a iao?een IO.

Oaeei /eiii, IO ia/?iee caeaoi? aei aia?aicaeaaeii? aeoioeyoe?? eao?ii?a
Ca2+, Sr2+, Mn2+ ? Cd2+, oaeaee?noue yei? aecia/a?oueny aioaeuei??th
a?ae?aoaoe?? eao?ii?a iaoae?a oa ?o nii??aeiai?noth aei NII—a?oi
Na2+-oi?ii?oa?a IO. ?acii c oei, i?eai?/aiiy aeeoaiiy IO ?iiaie La3+ oa
eoiieiiiae?aiee oa?aeoa? aeiciai? caeaaeiino? aoaeo?a Cd2+ ? La3+ ia
aeo?ae ?ii?a I+ c IO ia/?iee aeacothoue ia caeaoi?noue oeeo iaoae?a
aca?iiae?yoe c ?ioeie ooieoe?iiaeueieie nenoaiaie oeeo i?aaiae.

Aieea eaoiiiia ia?aoiaeieo i eoaeiicaiaeueieo iaoaeia ia
i?ioiiioi?-noeioeueiaaia aeeoaiiy oa ADP-oinoi?eethth/o caeaoi?noue
i?oioiiae??e

Aiaeiii, ui oaeaeeinoue aeeoaiiy IO, noeioeueiaaiiai i?ioiiioi?aie,
cie?aia n-o?eoei?iaoieneea?aiiieoeiaiiaeoaiieaiae?aciiii (CCCP) oa
2,4-aeeiio?ioaiieii (DNP), aecia/a?oueny iioaineaiinoth i?ioeania
ieeneaiiy o aeeoaeueiiio eaioethao oeeo i?aaiae. Iaie anoaiiaeaii, ui
CCCP (0,5 ieiieue/e) ?icei caieueoo? oaeaeeinoue aeeoaiiy IO (VCCCP)
iiney ?o i?aiieoaaoei? c ?iiaie Na2+, Sr2+ oa Mn2+ (100
ieiieue/e) (?en. 3). O oeueiio aeiaaeeo VCCCP iaei caea-

aeaea aiae aeaeo eaoiiiia iaoaeia (P > 0,05) i noaiiaeea 57,40?2,51-
58,20?1,15 iiieue I2/oa•ia aieea. ?ieo-aaoeiy IO c eaoiiiaie La3+ (50
ieiieue/e) noi?iaiaeaeoaaeanue nooo?-aei cieaeaiiyi VCCCP aei ?iaiy
28.95?1.28 iiieue I2/oa•ia aieea (P < 0,001). ?acii c oei CCCP (0,5 ieiieue/e), a oaeiae DNP (1 ieiieue/e) ia aieeaaee ia oaeaeeinoue aeeoaiiy IO iiney ?o aca?iiaei? c eaoiiiaie Nd2+ (50 ieieue/e). I?eaii/aiiy i?ioiiioi?- noeioeueiaaiiai aeeoaiiy IO iiae aieeaii iiiia Cd2+ i La3+ naiae/eoue i?i caeaoiinoue oeeo eaoiiiia iiaiaoaaoe i?ioeane iioioiiae?iaeueiiai ieeneaiiy. ?iaiaoth/ee aoaeo iiiia Cd2+ ?icaeaa?oueny iiney iiia?aaeiuei? noeioeyoei? aeeoaiiy, iaoiiaeaii? ?o aia?aicaeaaeiith aeoioeyoei?th a IO. Ii?ooaiiy iioioiiae?iaeueiiai ieeneaiiy iiae aieeaii eaoiiiia iaoaeia (50-100 ieiieue/e) nooo?ai iicia/a?oueny ia i?ioeanao ieeniiai oinoi?eethaaiiy o IO. Iieacaii, ui oaeaeeinoue ADP-noeioeueiaaiiai aeeoaiiy IO (VADP, 200 ieiieue/e) iiney ?o i?aiieoaaoei? c eaoiiiaie Na2+ i Sr2+ nooo?ai ia aiae?iciy?oueny aiae VADP ca aiaenooiinoi a na?aaeiaeui iieoaaoei? eaoiiiia iaoaeia (34,66±0,33 iiieue I2/oa•ia aieea) i noaiiaeoue aiaeiiaiaeii 32,63?1,95 i 32,36?1,87 iiieue I2/oa•ia aieea (P > 0,05). I?aiieoaaoeiy IO c eaoiiiaie Mn2+ oa La3+
noi?iaiaeaeoaaeanue ciaioaiiyi VADP aiaeiiaiaeii aei 26,16?2,25 oa
15,58?1,09 iiieue I2/oa•ia aieea, oiaoi ia 25 oa 31% (P < 0,05). ?ieoaaoeiy IO c eaoiiiaie Cd2+ i?ecaiaeeea aei iiaiiai i?eaii/aiiy ADP-noeioeueiaaiiai aeeoaiiy oeeo i?aaiae. Ca caeaoiinoth i?eaii/oaaoe i?ioiiioi?- oa ADP-noeioeueiaaia aeeoaiiy IO eaoiiie iaoaeia ooai?ththoue oaeee ?yae: Eaoiiie iaoaeia: Na2+ , Sr2+ < Mn2+ < La3+ < Cd2+ Ciaioaiiy VCCCP, %: 0 1 0 50 100 Ciaioaiiy VADP, %: 6 7 25 31 100 ?iaiaoth/ee aoaeo eaoiiiia iaoaeia ia VCCCP i VADP oinii ei?aeth? (r = 0,93 - 0,99) c eiinoaioaie noieeinoi eiiieaenia oeeo iaoaeia c SH-a?oiaie oeenoa?io (oaae. 2). Oaeei /eiii, eao?iie aeine?aeaeoaaieo iaoae?a, cie?aia Cd2+, La3+ ? /anoeiai Mn2+ i?eai?/othoue i?ioeane ieeneaiiy ? oinoi?eethaaiiy a IO ia/?iee. Caeaoi?noue eao?ii?a iaoae?a i?eai?/oaaoe i?ioeane ieeneaiiy ? oinoi?eethaaiiy a IO aecia/a?oueny ?o nii??aeiai?noth aei n??eiai?nieo e?aaiae?a oeeo i?aaiae. Oaaeeoey 2 Caeaaeiinoue iiaiaoth/eo aoaeoia eaoiiiia iaoaeia ia i?ioiiioi?- oa ADP-noeioeueiaaia aeeoaiiy i?oioiiae??e aiae ?o oiceei-oiii/ieo aeanoeainoae Ia?aiao?e Eiaoioei?io ei?aeyoei?, raeeoaiiy i?oioiiae??e?ii. ?aaeion, ii?Ii aiae?, eeae/iieuelgK1 iaoae-aoeaoaolgK1 iaoae-AsplgE1 iaoae-Cys Ciaioaiiy VCCCP, %0,15 p=0,220,32 p=0,590,67 p=0,810,66 p=0,750,93 p=0,98Ciaioaiiy VADP, %-0,17 p=0,220,07 p=0,150,50 p=0,610,63 p=0,750,99 p>0,99I?eiioea. ? — ai?iaiaeiinoue eiaoioei?ioa ei?aeyoei?.

Iaoaiicie aei? eao?ii?a eoaeiicaiaeueieo ? ia?ao?aeieo iaoaeia ia

eaeoeieaeoioeth/o ooieoeith iioioiiae?ie

Aiaeiii, ui aeniea eaeueoeieaeoioethth/a caeaoiinoue IO ia? aaaeeeaa
cia/aiiy o iiaeo?eiaiii eaeueoei?aiai aiiainoaco eeioeie (Gunter,
Pfeiffer, 1990, Einoa?ei, Ao?aeuaa, 1993) oa o ?aaoeyoei?
ooieoeiiioaaiiy oeeo i?aaiae (McCormack et al., 1990). Anoaiiaeaii, ui
eaoiiie Sr2+ (20-200 ieiieue/e), Cd2+ i La3+ (2-50 ieiieue/e), aianaii o
noniaicith IO iaeii/anii c eaoiiiaie Na2+ (30 ieiieue/e),
i?eaii/othoue iiaeeiaiiy iiiia Na2+ oeeie i?aaiaeaie (?en. 4).

?en. 4. Caeaaei?noue oaeaeeino? iiaeeiaiiy Na2+ (V, iiieue Na2+/oa•ia
aieea) iioioiiae?iyie a?ae eiioeaio?aoe?? eaoiiiia iaoaeia ([Men+],
ieiieue/e) o noniaic?? oeeo i?aaiae.

Aeiciaa caeaaeiinoue aoaeoia eaoiiiia Sr2+, Cd2+ i La3+ iaea
aeniiiaioeiaeueiee oa?aeoa?. Eaoiiie Mn2+ o eiioeaio?aoeiyo 10-50
ieiieue/e oaeiae i?eaii/oaaee iiaeeiaiiy eaeueoeith iioioiiae?iyie
ia/iiee. I?ioa caieueoaiiy eiioeaio?aoei? iiiia Mn2+ o noniaici? IO a
aeiaiaciii 50-200 ieiieue/e noi?iaiaeaeoaaeinue ciaioaiiyi ?o
iiaiaoth/iai aieeao ia iiaeeiaiiy iiiia Na2+ i?oioiiae??yie. Iiae?aiee
aoaeo aoa a?aei?/aiee ? ?ioeie aaoi?aie (Hughes , Exton , 1983, Allshire
et al., 1985) ye? aeyaeee caeaoi?noue ?ii?a Mn2+ noeioethaaoe iiaeeiaiiy
Na2+ IO ia/?iee uo??a oa iiceo ii?nueei? naeiee. Eiiai?ii, iiie Mn2+ o
aenieeo eiioeaio?aoeiyo caeaoii aca?iiaeiyoe c eaoiiica’ycoth/eie
a?oiaie Na2+-oiiii?oa?a, ui noi?iaiaeaeo?oueny iiaeeoieaoei?th eiai
i?iaiaeiinoi.

E?i?? aeiciai? caeaaeiino? ?ia?aoth/eo aoaeo?a eao?ii?a Sr2+, Mn2+ oa
La3+ ia iiaeeiaiiy Na2+ i?oioiiae??yie o eii?aeeiaoao Oaaa
ia?aoeiathoue a?nue i?aeeiao o oi/eao, aeecueeeo aei iaeeieoe? (?en.
5). Oea aeaco? ia eiieo?aioiee oei ?ia?aoaaiiy oeeie eao?iiaie
o?ainii?oo Na2+ o IO. O aeiaaeeo c ?iiaie Cd2+

?en. 5 E?iaoe/iee aiae?c ?ia?aoth/iai aieeao eao?ii?a iaoae?a ia
iiaeeiaiiy Na2+ i?oioiiae??yie ia/?iee a nenoai? eii?aeeiao Oaaa (a) oa
Eaeio?aa?a-Aa?ea (a). a) 1/[Ian+] — iaa?iai? aaee/eie eiioeaio?aoe??
eao?ii?a iaoae?a o noniaic?? i?oioiiae??e, V0/(V0-Vi) — iaa?iai?
aaee/eie /anoee ia?ia?aiaaii? oaeaeeino? iiaeeiaiiy Na2+ i?oioiiae??yie;
a) 1/[Ca2+] — iaa?iai? cia/aiiy eiioeaio?aoe?e eao?ii?a Na2+ o noniaic??
i?oioiiae??e; 1/V — iaa?iai? cia/aiiy oaeaeeino? iiaeeiaiiy eaeueoe?th
i?oioiiae??yie.

eii?aeeiaoe oi/ee ia?aoeio io?eiaii? e?i?? c a?nnth i?aeeiao cia/ii
a?ae??ciythoueny a?ae iaeeieoe?, ui na?ae/eoue i?i ?ioee oei
?ia?aoaaiiy.

Aecia/aiiy oeio iiaiaoaaiiy eao?iiaie Cd2+ o?ainii?oo Na2+ a IO
i?iaiaeeee a?aoi/iei iaoiaeii o eii?aeeiaoao Eaeioiaa?a-Aa?ea. Aeey
ii??aiyiiy o oe?e nenoai? eii?aeeiao aoa ioe?iaiee oei ?ia?aoaaiiy
iiaeeiaiiy Na2+ i?oioiiae??yie eao?iiaie Mn2+. Aeyaeaia iiae aieeaii
Mn2+ (50 ieiieue/e) caieueoaiiy E0,5 iiaeeiaiiy Na2+ iioioiiae?iyie c
56,99 aei 113,68 ieiieue/e oa aiaenooiinoue nooo?aeo ciii Vmax oeueiai
i?ioeano i?aeoaa?aeaeo? eiieo?aioiee oa?aeoa? iiaiaoaaiiy. Iiae aieeaii
Cd2+ (10 ieiieue/e) E0,5 iiaeeiaiiy eaeueoeith IO caieueooaaeanue c
56,99 aei 67,37 ieiieue/e, a Vmax oeueiai i?ioeano ciaiooaaeanue c
913,79 aei 532,50 iiieue Na2+/oa•ia aieea, ui aeaco? ia ciioaiee oei
iiaiaoaaiiy. Iiaeeeaei iaoaiiciii oeueiai ? aeyaeaia iaie i?eaii/aiiy
iiae aieeaii iiiia Cd2+ i?ioeania ieeneaiiy o aeeoaeueiiio eaioethao IO.

Ii?iaiyiiy iiaiaoth/iai aieeao eaoiiiia iaoaeia ia eaeueoeieaeoioethth/o
caeaoiinoue IO i?iaiaeeee ia iniiai ?ia?a?oi?ieo eiinoaio (Ki), yei
aecia/aee iaoiaeii eiiaa?ecaoei? aeniiiaioeiaeueieo e?eaeo aeica-aoaeo a
eii?aeeiaoao Oaaa (aeea. ?en. 5). Aeey ii??aiyeueiiai aiae?co aoea
aeei?enoaia oaeiae Ki o?ainii?oo Na2+ a IO ?iiaie Aa2+, aecia/aia
E.Aea?iaiii ?c ni?aaaoi?aie (Akerman et al, 1977). Anoaiiaeaii, ui ca
ciaioaiiyi iiaiaoth/iai aieeao ia iiaeeiaiiy eaeueoeith iioioiiae?iyie
(Ei, ieiieue/e) eaoiiie iaoaeia ooai?ththoue oaeee ?yae:

Eaoiiie iaoaeia: La3+ < Cd2+ < Mn2+ < Sr2+ < Ba2+ Ki, ieiieue/e: 2,11 10,36 49,29 66,43 70,00 Io?eiaiee ?yae oa?aeoa?eco?oueny oiniith iaa?iaiith ei?aeyoei?th (oaae. 3) c aioaeueii?th aiae?aoaoei? (r = -0,78) iiiia iaoaeia oa eiinoaioaie noieeinoi ?o eiiieaenia c ea?aieneeueieie a?oiaie aoeaoaoo (r = -0,96) oa ania?aaiiiai? eeneioe (r = - 0,91). Oea aeaco? ia aaaeeeao ?ieue i?ioeania aeaaiae?aoaoei? eaoiiiia iaoaeia oa ?o aca?iiaei? c ea?aieneeueieie a?oiaie Na2+-oiiii?oa?a o iaoaiiciao o?aineieaoei? Na2+ /a?ac iaia?aio IO oa o i?eaii/aii? oeueiai i?ioeano eaoiiiaie iaoaeia. Ia ei?enoue oeueiai na?ae/eoue aenieee ai?no (24%) aeeea?aiiiaeo ai?iieeneio o neeaae? Na2+-o?ainii?oiiai ae?eii?ioa?io IO na?oey aeea (Ie?iiiaa, Ooaoaaa, 1989). Oaaeeoey 3 Caeaaeiinoue iiaiaoth/eo aoaeoia eaoiiiia iaoaeia ia iiaeeiaiiy Na2+ iioioiiae?iyie aiae oiceei-oiii/ieo aeanoeainoae iiiia iaoaeia. Eiiaoe/ii ia?aiao?e Eiaoioei?io ei?aeyoei?, riiaiaoaaiiy iiaeeiaiiy Na2+ i?oioiiae??yie?ii. ?aaeion, ii?Ii aiae?, eeae/iieuelgK1 iaoae-aoeaoaolgK1 iaoae-AsplgE1 iaoae-Cys Ki iiaeeiaiiy Na2+, ieiieue/e-0,26 p=0,35-0,78 p=0,90-0,96 p>0,98-0,91 p=0,97-0,72 p=0,83I?eiioea. ? — ai?iaiaeiinoue eiaoioei?ioa
ei?aeyoei?.

Oaeei /eiii, eao?iie iaoae?a ?ia?aothoue o?ainii?o Na2+ a IO ia/?iee ca
eiieo?aioiei (Sr2+, Mn2+, La3+) oa ci?oaiei (Cd2+) oeiii. Caeaoi?noue
eao?ii?a iaoae?a ?ia?aoaaoe o?ainii?o Na2+ a IO aecia/a?oueny
aioaeuei??th ?o a?ae?aoaoe?? oa nii??aeiai?noth aei ea?aieneeueieo a?oi
Na2+-oi?ii?oa?a oeeo i?aaiae.

Aieea eao?ii?a eoaeiicaiaeueieo ? ia?ao?aeieo iaoaeia ia ianiaoeeoi/io

i?iieeiinoue aioo?ioiuei? iaia?aie iioioiiae?ie

Aiaeiii (Zoratti , Szabo, 1995), ui iaa?iiaaeaeaiiy eaeueoeith o IO ca
iaaieo oiia i?ecaiaeeoue aei ia?aoiaeo ?o aioo?ioiuei? iaia?aie o noai
aeniei? ianiaoeeoi/ii? i?iieeiinoi (AII), iaaooaiiy oeeo i?aaiae, ui
noi?iaiaeaeo?oueny ao?aoith ieie caeaoiino? aei ieeniiai
oinoi?eethaaiiy. Ii?yae c oei aeai? i?i aieea eao?ii?a iaoae?a ia noai
i?iieeiino? iaia?aie IO, ui iiaea aooe iaeiei ?c iaoai?ci?a ?aae?caoe??
?o aieeao ia ooieoe?iioaaiiy oeeo i?aaiae, iaiaaeai?. Iaie anoaiiaeaii,
ui iiaeoeiaaia Na2+ (30-100 ieiieue/e) iaaooaiiy IO aeeth/a? oace
iiaieueiiai (eaa-oaca) oa oaeaeeiai iaaooaiiy oeeo i?aaiae (?en. 6).
Ciaioaiiy naioeiiiaeeiaiiy noniaici? IO, ui naiae/eoue i?i ?o iaaooaiiy,
aeinyaaei 0,1780,003 iae. aeno/ia a?eea (P < 0,001), a eiai oaeaeeinoue - 0,099??0,008 iae. aeno./oa•ia aieea. Eaoiiie Cd2+ (15-50 ieiieue/e) oaeiae iiaeoeoaaee iaaooaiiy IO, yea, i?ioa, oa?aeoa?ecoaaeinue aiaenooiinoth eaa-oace, iaioith aiieiooaeith oa o?eaaeinoth i?ioeano. Oae, iaeneiaeueia aiieiooaea Cd2+-iiaeoeiaaiiai iaaooaiiy, aeinyaaea 0,097 0,002 iae. aeno/ia a?eea (P <0,05), a eiai oaeaeeinoue - 0,148?0,006 iae. aeno./oa•ia aieea. Eaoiiie Sr2+ (50, 100 ieiieue/e), Mn2+ (50, 200 ieiieue/e) oa La3+ (2, 50 ieiieue/e) ia i?ioyci 7-8 oa. ia aeeeeeaee nooo?aeo ciii naioei-iiaeeiaiiy noniaici? IO (P > 0,05).
I/aaeaeii, ui eaoiiie Sr2+, Mn2+ oa La3+ o aeineiaeaeoaaieo
eiioeaio?aoeiyo ia aeeeeeathoue ia?aoiaeo aioo?ioiuei? iaia?aie IO o
noai AII.

?en. 6. *aniaa eiiaoeea aieeao eaoiiiia iaoae?a (50 ieiieue/e) ia
naioeiiiaeeiaiiy noniaici? iioioiiae?ie (?E520, iae. aeno./ia a?eea).

Eaoiiie Cd2+ o eiioeaio?aoeiyo 15 i 50 ieiieue/e nooo?ai
ciiithaaee eiiaoeeo Na2+-iiaeoeiaaiiai iaaooaiiy IO, iaaeeaeath/e ?? aei
eiiaoeee aeaniiai aieeao (oaae. 4). Oae, eaoiiie Cd2+ (50 ieiieue/e)
ciaiooaaee aiieiooaeo Na2+-iiaeoeiaaiiai (30 ieiieue/e) iaaooaiiy IO c
0,178?0,003 aei 0,076?0,005 iae. aeno./ia aieea (P < 0,001), a oaeiae o?eaaeinoue eaa-oace oa caaaeueio o?eaaeinoue iaaooaiiy. Aiaenooiinoue aaeeoeaiinoi aoaeoia iiiia Ca2+ i Cd2+ ia iaaooaiiy IO naiae/eoue, ui ?o aieea ?aaeico?oueny /a?ac oi?ioaaiiy iaeieo i oeo aea ii? o iaia?aii oeeo i?aaiae. I?ioa iniaeeainoi eiiaoeee Na2+- i Nd2+-iiaeoeiaaiiai iaaooaiiy aeacothoue ia ?icii iaoaiicie iiioeiaoei? oi?ioaaiiy oeeo ii?, a iniiai /iai iiaea eaaeaoe iaiaeeiaeiaa nii?iaeiaiinoue eaoiiiia oeeo iaoaeia aei ea?aieneeueieo i noeueoaiae?eeueieo a?oi aiieiaaiaeia. Eaoiiie Sr2+ i Mn2+ o eiioeaio?aoei? 50-200 ieiieue/e nooo?ai i?eaii/oaaee Na2+-iiaeoeiaaia iaaooaiiy IO (aeea. oaae. 4). ?iaiaoth/ee aoaeo eaoiiiia Sr2+ i Mn2+ caieueooaaany ic c?inoaiiyi ?o eiioeaio?aoei? o noniaici? IO. Oae, eaoiiie Mn2+ o eiioeaio?aoei? 50 ieiieue/e caieueooaaee o?eaaeinoue eaa-oace Na2+- iiaeoeiaaiiai (30 ieiieue/e) iaaooaiiy IO aiae 268,50±32,03 aei 562,00±72,38 n (P < 0,01), a o eiioeaio?aoei? 200 ieiieue/e iiaiinoth caiiaiaaee ?icaeoeo oeueiai i?ioeano. Aiaeiai/ii, oi/a i iaio ae?aaeaii ciiie, niinoa?iaaeenue iiae aieeaii iiiia Sr2+. ?iaiaoth/ee aieea eaoiiiia Sr2+ i Mn2+ ia Na2+-iiaeoeiaaia iaaooaiiy IO ciaiooaaany ic caieueoaiiyi eiioeaio?aoei? Na2+ o na?aaeiaeui iieoaaoei?. Oae, eaoiiie Mn2+ o eiioeaio?aoei? 100 ieiieue/e caieueooaaee o?eaaeinoue eaa-oace iaaooaiiy IO, iiaeoeiaaiiai Na2+ o eiioeaio?aoeiyo 30 oa 100 ieiieue/e, aiaeiiaiaeii ia 110 (P < 0,01) oa 32% (P < 0,05). Aiaeiai/ii ciiie niinoa?iaaeenue iiae aieeaii Sr2+. Iaaaaeaii ?acoeueoaoe naiae/aoue Oaaeeoey 4 Aieea eaoiiiia iaoaeia ia Na2+-iiaeoeiaaia iaaooaiy iioioiiae?ie ia/iiee (M??m, n = 4) Eiioeaio?aoeiy eaoiiiia iaoae?a, ieiieue/eAiaeiinii ciiie ia'?io iioioiiae??e, ?A520 iae. aeno./oa•ia aieeaAiaeiinia oaeaeeinoue iaaooaiiy i?oioiiae??e, ?A520 iae. aeno. /oa•ia aieeaCaaaeueia o?eaaeinoue i?ioeano, cO?eaaeinoue eaa-oace, cCa2+,30 0,178±0,0030,080±0,008839,63 ±4,31268,50±32,03Ca2+,100 0,166±0,0040,099±0,008750,69±43,67258,60±31,74Cd2+,50 0,065±0,0020,089±0,004283,20±23,0323,72±0,85Ca2+,30 Cd2+,500,076±0,005 P1<0,0010,111±0,011 P1>0,05376,50±19,19
P1<0,00128,20±2,24 P1<0,001Ca2+,100 Cd2+,500,090±0,007 P1<0,0010,107±0,013 P1>0,05451,80±15,40 P1<0,00131,00±1,00 P1<0,001Ca2+,30 Sr2+,500,175±0,010 P1>0,050,038±0,004
P1<0,011069,80±49,80 P1<0,01225,75±43,39 P1>0,05Ca2+,30
Sr2+,1000,159±0,016 P1>0,05 P2>0,050,032±0,001 P1<0,01 P2<0,051241,00±32,05 P1<0,01 P2<0,01440,25±62,85 P1<0,01 P2<0,05Ca2+,100 Sr2+,500,163±0,007 P1>0,05 P3>0,050,058±0,005
P1<0,01 P3<0,01692,50±73,33 P1>0,05 P3<0,01237,75±21,51 P1>0,05
P3>0,05Ca2+,30 Mn2+,500,184±0,001 P1>0,050,099±0,017
P1>0,051115,00±32,79 P1<0,01562,00±72,38 P1<0,01Ca2+,30 Mn2+,2000,008±0,001 P1<0,01 P2<0,01I a a o o a i i y a ? ae n o o i ?Ca2+,100 Mn2+,500,166±0,003 P1>0,05 P3<0,010,088±0,005 P1>0,05
P3>0,05891,75±41,33 P1<0,05 P3<0,01341,25±30,10 P1<0,05 P3<0,05I?eiioea. P1 - aeinoiai?iinoue ?icieoei iieacieeia Na2+-iiaeoeiaaiiai iaaooaiiy i?oi-oiiae??e o i?enooiinoi oa ca aiaenooiinoi o na?aaeiaeui iiiia iaoaeia; P2 - aeinoiai?iinoue ?icieoei iieacieeia Na2+-iiaeoeiaaiiai (30 ieiieue/e) iaaooaiiy i?oioiiae??e o i?enooiinoi ?icieo eiioeaio?aoeie iiiia iaoaeia; P3 - aeinoiai?iinoue ?icieoei iieacieeia iaaooaiiy i?oioiiae??e, iiaeoeiaaiiai ?icieie eiioeaio?aoeiyie Na2+ (30 i 100 ieiieue/e) o i?enooiinoi iiiia iaoaeia. i?i eiieo?aioiee oa?aeoa? i?eaii/aiiy Na2+-iiaeoeiaaiiai caieueoaiiy ianiaoeeoi/ii? i?iieeiinoi iaia?aie IO eaoiiiaie Sr2+ i Mn2+. A iniiai oeueiai eaaeeoue, iiiai?ii, i?eaii/aiiy oeeie eaoiiiaie aeoioeyoei? eaoiiiia Na2+ iioioiiae?iyie oa ?o aca?iiaeiy c Na2+-ca'ycoth/eie a?oiaie ii?iooai?thth/eo aieeia iaia?aie IO Oaeei /eiii, eao?iie Na2+ ? Cd2+ ?iaeoeothoue ia?ao?ae aioo??oiuei? iaia?aie IO o noai AII. Iniaeeaino? e?iaoeee Na2+- ? Cd2+-?iaeoeiaaiiai iaaooaiiy IO na?ae/aoue i?i a?aei?ii?noue iaoai?ci?a ?o aieeao ia noai i?iieeiino? iaia?aie oeeo i?aaiae, ui, eiia??ii, iaoiiaeaii ?o iaiaeeiaeiaith nii??aeiai?noth aei NII- ? SH-a?oi. Eao?iie Sr2+, Mn2+ i La3+ ia ?iaeoeothoue iaaooaiiy IO. Sr2+ ? Mn2+ i?eai?/othoue Na2+-?iaeoeiaai? ci?ie i?iieeiino? aioo??oiuei? iaia?aie IO ca eiieo?aioiei oeiii. A E N I I A E E 1. I?oioiiae??? ia/?iee caeaoi? aei aia?aicaeaaeii? aeoioeyoe?? eao?ii?a ?yaeo eoaeiicaiaeueieo (Ca2+, Sr2+) i ia?aoiaeieo (Mn2+, Cd2+) iaoaeia, ui noi?iaiaeaeo?oueny noeioeyoe??th ?o aeeoaiiy oa aeoiaeii I+ c oeeo i?aaiae. Eaoiiie La3+ (50-400 ieiieue/e) ia ci?iththoue, a o a?aenooiino? aeciaaiiiai oinoaoo /anoeiai i?eai?/othoue oaeaeeinoue aeeoaiiy i?oioiiae??e, oi/a ? noeioeththoue aeo?ae I+ c oeeo i?aaiae. 2. Caeaoi?noue eao?ii?a iaoae?a noeioethaaoe aeeoaiiy i?oioiiae??e (Vmax), iaoiiaeaia ?o o?aineieaoei?th a iao?een oeeo i?aaiae, aecia/a?oueny aioaeueii?th aiae?aoaoei? eao?ii?a iaoae?a oa ?o nii?iaeiaiinoth aei ea?aieneeueieo a?oi Na2+-oiiii?oa?a i?oioiiae??aeueii? iaia?aie ? niaaea? o oaeiio ?yae?: Ca2+ > Sr2+ > Cd2+
> Mn2+ >> La3+.

3. Eao?iie Ca2+, Sr2+ i Mn2+ (100 ieiieue/e) ia aieeaathoue, a eaoiiie
Cd2+ i La3+ (50 ieiieue/e) i?eaii/othoue aeeoaiiy i?oioiiae??e,
noeioeueiaaia i?ioiiioi?aie (NNN?, 0,5 ieiieue/e; DNP, 10 ieiieue/e), ui
naiae/eoue i?i aaciina?aaei? iiaiaoaaiiy ieie i?ioeania ieeneaiiy a
aeeoaeueiiio eaioethao oeeo i?aaiae. A oeieiio, caeaoiinoue eaoiiiia
iaoaeia i?eaii/oaaoe i?ioeane ieeneaiiy a aeeoaeueiiio eaioethao
i?oioiiae??e caieueoo?oueny o oaeiio ii?yaeeo: Na2+, Sr2+, Mn2+ < La3+ < Cd2+. 4. Eaoiiie Mn2+ (100 ieiieue/e), Cd2+ i La3+ (50 ieiieue/e) i?eaii/othoue ADP-noeioeueiaaia aeeoaiiy i?oioiiae??e, a eao?iie Na2+ ? Sr2+ (100 ieiieue/e) ia aieeaathoue ia oeae i?ioean. Caeaoiinoue iiiia iaoaeia i?eaii/oaaoe ADP-noeioeueiaaia aeeoaiiy i?oioiiae??e c?inoa? a ?yae?: Na2+, Sr2+ < Mn2+ < La3+ < Cd2+. 5. Caeaoiinoue eaoiiiia iaoaeia i?eaii/oaaoe i?ioeane ieeneaiiy i oinoi?eethaaiiy a i?oioiiae??e aecia/a?oueny ?o nii?iaeiaiinoth aei SH- a?oi iae?iiieaeoe aeeoaeueiiai eaioethaa oeeo i?aaiae. 6. Eao?iie Mn2+, Sr2+ i La3+ iiaiaothoue o?ainii?o Na2+ o i?oioiiae??? ca eiieo?aioiei, a eaoiiie Cd2+ - ca ciioaiei oeiii. ?ia?a?oi?ia eiinoaioa (Ki, ieiieue/e) o?ainii?oo Na2+ o i?oioiiae??? aeey eaoiii?a iaoaeia caieueooaaeanue o oaeiio ii?yaeeo: La3+ < Cd2+ < Mn2+ < Sr2+. Caeaoiinoue eaoiiiia iaoaeia iiaiaoaaoe o?ainii?o Na2+ a i?oioiiae??? cia/iith ii?ith aecia/a?oueny aioaeueii?th ?o aiae?aoaoei? oa nii?iaeiaiinoth aei ea?aieneeueieo a?oi Na2+-oiiii?oa?a oeeo i?aaiae. 7. Eaoiiie Na2+ (30-100 ieiiieue/e) i Cd2+ (15-50 ieiieue/e) iiaeoeothoue ia?ao?ae aioo??oiuei? iaia?aie i?oioiiae??e o noai aeniei? ianiaoeeo?/ii? i?iieeiino? ca ??cieie iaoai?ciaie, i?ioa oeyoii oi?ioaaiiy iaeieo ? oeo aea ii?. Iaeaieueo iiiai?ii a iniiai oeueiai eaaeeoue iaiaeeiaeiaa nii?iaeiaiinoue eaoiiiia Na2+ i Cd2+ aei ea?aieneeueieo i noeueoaiae?eeueieo a?oi iae?iiieaeoe iaia?aie i?oioiiae??e. 8. Eaoiiie Sr2+ (50-100 ieiieue/e), Mn2+ (50-200 ieiieue/e) oa La3+ (2-50 ieiieue/e) ia iiaeoeothoue iaaooaiiy i?oioiiae??e ia/iiee. ?acii c oei aiie i?eaii/othoue Na2+-iiaeoeiaaii ciiie i?iieeiinoi aioo?ioiuei? iaia?aie i?oioiae??e ca eiieo?aioiei oeiii. A iniiai oeueiai eaaeeoue, iiiai?ii, i?eaii/aiiy oeeie eaoiiiaie aeoioeyoei? eaoiiiia Na2+ iioioiiae?iyie oa ?o aca?iiaeiy c Na2+-ca'ycoth/eie a?oiaie ii?iooai?thth/eo aieeia iaia?aie i?oioiiae??e. 9. Eaoiiie ?yaeo eoaeiicaiaeueieo (Ca2+, Sr2+) i ia?aoiaeieo (Mn2+, Cd2+, La2+) iaoaeia nooo?ai aieeaathoue ia ooieoeiiioaaiiy i?oioiiae??e ia/iiee, cie?aia ia aeeoaiiy, ADP-oinoi?eethth/o oa eaeueoeieaeoioethth/o ooieoei? i?oioiiae??e, a oaeiae ia noai i?iieeiinoi aioo?ioiuei? iaia?aie oeeo i?aaiae. Caeaoiinoue eaoiiiia iaoaeia aca?iiaeiyoe c ?icieie ooieoeiiiaeueieie nenoaiaie i?oioiiae??e iaiaeeiaeiaa i aecia/a?oueny ?o oiceei-oiii/ieie aeanoeainoyie: aioaeueii?th aiae?aoaoei?, nii?iaeiaiinoth aei ea?aieneeueieo i noeueoaiae?eeueieo a?oi iae?iiieaeoe. Oea noai?th? iiaeeeaino? aeey iai?aaeaiiai iiooeo aioeaeio?a aei iaoae?a c ??ciith nii??aeiai?noth aei eenia- ? n??eiai?nieo e?aaiae?a ee?oeie. NIENIE IOAE?EAOe?E CA OAIITH AeENA?OAOe? 1. Aeoaeoeueeee E.I., Aiaeaie/ E.N. Aieea eaoiiiia ia?aoiaeieo iaoaeia ia aeeoaiiy i i?iaeoeoaaiiy I+ iioioiiae?iyie ia/iiee // Oe?. aiioii. aeo?i. - 1996. - O. 68, N 5. - N. 59-63. 2. Aeoaeoeueeee E.I., Aiaeaie/ E.N., Iieioithe N.ss. ?ieue eaeueoeieo?ainii?oieo nenoai i iioioiiae?ie o ii?ooaiii aecioeeoico nae?aoi?ieo eeioei oeoiea eaoiiiaie ia?aoiaeieo iaoaeia // Aeooae. i?iae. iaaeeoeeie, aiieiai?, aaoa?aia?i? oa nieuenueeiai ainiiaea?noaa. - Eueaia: Ai/a, 1997. - N. 59-62. 3. Aiaeaie/ E.N., Aeoaeoeueeee E.I. Aieea eaoiiiia eoaeiicaiaeueieo i ia?aoiaeieo iaoaeia ia ianiaoeeoi/io i?iieeiinoue aioo?ioiuei? iaia?aie iioioiiae?ie // Aenia?eiaioaeueia oa eeiii/ia oiciieiaiy i aiioiiiy. - 1998. - N 1. - N. 5-9. 4. Aeoaeoeueeee E.I., Aiaeaie/ E.N. Aeineiaeaeaiiy aieeao eaoiiiia ia?aoiaeieo iaoaeia ia Na2+-noeioeueiaaia aeeoaiiy iioioiiae?ie ia/iiee uo?ia // Iaoa?iaee 1-ai c'?caeo Oe?a?inueeiai aiioice/iiai oiaa?enoaa. - Ee?a. - 1994. - N. 90 - 91. 5. Aeoaeoeueeee E.I., Aiaeaie/ E.N., Naaaaeao A.?. Aeineiaeaeaiiy aieeao eaoiiiia ia?aoiaeieo iaoaeia ia ooieoeiiiaeueio aeoeaiinoue nae?aoi?ieo eeioei oeoiea. // Oac. aeii. XIV c'?caeo Oe?a?inueeiai oiciieiai/iiai oiaa?enoaa ii. ?.I. Iaaeiaa. - Ee?a. - 1994. - N. 157. 6. Aeoaeoeueeee E.I., Aiaeaie/ E.N. Aeineiaeaeaiiy aaeaioeaieo ?aaeoeie iioioiiae?ie ia/iiee uo?ia i?e iioieneeaoeiyo iaoaeaie // Iao. iaoe.-i?aeo. neii. "Iaaeeei-aiieiai/ii i?iaeaie aaeaioaoei? a no/anieo oiiaao inioaaiiy i?aaiicio" (14-16 aa?aciy 1995 ?., i. Eociaoeianuee). - Eueaia, 1995. - N. 19. 7. Aeoaeoeueeee E.I., Aiaeaie/ E.N., Naaaaeao A.?., Neithe C.A. Aieea eaoiiiia ia?aoiaeieo iaoaeia ia nenoaie iiaeo?eiaiiy eaeueoei?aiai aiiainoaco nae?aoi?ieo eeioei oeoieo // Aenia?eiaioaeueia oa eeiii/ia oiciieiaiy. - Eueaia, 1995. - N. 148-149. 8. Vovkanych L.S. Investigation of the mechanisms of metals` cations effect on respiration and H+ release of rat liver mitochondria // Book of abstracts of the 4-th International students` scientific conference. - Gdansk, 1996. - P. 6. 9. Vovkanych L.S., Dubitsky L.O. Investigation of the mechanisms of metals` cations effect on respiration of rat liver mitochondria // 9-th European bioenergetic conference. - August 17-22, EBEC Reports. - Biochim. Biophys. Acta. - 1996, V. 9. - P.192. 10. Aeoaeoeueeee E.I., Aiaeaie/ E.N., Iieioithe N.ss. Aeineiaeaeaiiy ?iei eaeueoeieo?ainii?oieo nenoai a ii?ooaiii ooieoeiiiaeueiie aeoeaiinoi nae?aoi?ieo eeioei eaoiiiaie iaoaeia // Oac. aeii. VII Oe?. aiioii. c'?caeo. - Ee?a, 1997. - N. 40-41. 11. Aiaeaie/ E.N., Aeoaeoeueeee E.I. Aieea eaoiiiia iaoaeia ia aeeoaiiy oa eaeueoeieo?ainii?oii i?ioeane o iioioiiae?iyo ia/iiee uo?a // Iaoa?iaee XV c'?caeo Oe?a?inueeiai oiciieiai/iiai oiaa?enoaa (Aeiiaoeuee, 1998). - Oiciie. aeo?i. - 1998. - O. 44, N 3. - N. 156. AIIOAOe?ss AIAEAIE* E.N. Aieea eaoiiiia eoaeiicaiaeueieo i ia?aoiaeieo iaoaeia ia ooieoeiiioaaiiy iioioiiae?ie ia/iiee uo?ia. - ?oeiien. Aeena?oaoeiy ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa aiieiai/ieo iaoe ca niaoeiaeueiinoth 03.00.02 - a?io?ceea. - Ee?anueeee oi?aa?neoao ?iai? Oa?ana Oaa/aiea, Ee?a, 1999. Aeena?oaoe?th i?enay/aii aea/aiith iaoai?ci?a ae?? eao?ii?a iaoae?a (EI), cie?aia Ca2+, Sr2+, Mn2+, Cd2+ ? La3+, ia aeeoaiiy, ADP-oinoi?eethth/o ? Na2+-aeoioethth/o ooieoe?? i?oioiiae??e (IO) ia/?iee, oa ia noai ianiaoeeo?/ii? i?iieeiino? ?o aioo??oiuei? iaia?aie. Anoaiiaeaii, ui IO caeaoi? aei aia?aicaeaaeii? aeoioeyoe?? EI, oaeaee?noue yei? aecia/a?oueny aioaeuei??th a?ae?aoaoe?? EI oa ?o nii??aeiai?noth aei NII- a?oi Na2+-oi?ii?oa?a IO. I?eai?/aiiy EI i?ioean?a ieeneaiiy noano?ao?a oa ieeniiai oinoi?eethaaiiy o IO ei?aeth? c eiinoaioaie no?eeino? eiiieaen?a oeeo iaoae?a c SH- a?oiaie oeenoa?io. EI ?ia?aothoue o?ainii?o Na2+ o IO ca eiieo?aioiei (Sr2+, Mn2+, La3+) oa ci?oaiei oeiii (Cd2+). Ae?aaeai?noue ?ia?aoaaiiy aecia/a?oueny aioaeuei??th a?ae?aoaoe?? EI oa ?o nii??aeiai?noth aei NII- a?oi Na2+-oi?ii?oa?a. Ca2+ ? Cd2+, ia a?aei?io a?ae Sr2+, Mn2+ oa La2+, ?iaeoeothoue ca?eueoaiiy ianiaoeeo?/ii? i?iieeiino? iaia?aie IO. Sr2+ ? Mn2+ caeaoi? eiieo?aioii i?eai?/oaaoe oeae i?ioean. Ioaea, caeaoi?noue EI aca?iiae?yoe c ??cieie ooieoe?iiaeueieie nenoaiaie IO iaiaeeiaeiaa ? aecia/a?oueny ?o o?ceei-o?i?/ieie aeanoeainoyie, cie?aia aioaeuei??th a?ae?aoaoe?? oa nii??aeiai?noth aei eenia- ? n??eiai?nieo a?oi a?ie?aaiae?a, ui iiaea aooe aeei?enoaia aeey iai?aaeaiiai iiooeo aioeaeio?a aei ??cieo iaoae?a. Eeth/ia? neiaa: i?oioiiae??? ia/?iee, eao?iie iaoae?a, aeeoaiiy, ieenia oinoi?eethaaiiy, iiaeeiaiiy eaeueoe?th, ianiaoeeo?/ia i?iieei?noue iaia?aie. AIIIOAOeEss AIAEAIU* E.N. Aeeyiea eaoeiiia uaei/iicaiaeueiuo e ia?aoiaeiuo iaoaeeia ia ooieoeeiie?iaaiea ieoioiiae?ee ia/aie e?un. - ?oeiienue. Aeenna?oaoeey ia nieneaiea o/iie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.02 - aeioeceea. - Eeaaneee oieaa?neoao eiaie Oa?ana Oaa/aiei, Eeaa, 1999. Aeenna?oaoeey iinayuaia eco/aieth iaoaieciia aeeyiey eaoeiiia iaoaeeia (EI), a /anoiinoe Ca2+, Sr2+, Mn2+, Cd2+, La3+, ia aeuoaiea, ADP-oinoi?eee?othuoth e Na2+-aeeoioee?othuoth ooieoeee ieoioiiae?ee (IO) ia/aie, a oaeaea ia ninoiyiea ianiaoeeoe/aneie i?iieoeaaiinoe eo aioo?aiiae iaia?aiu. Onoaiiaeaii, /oi IO iaeaaeatho niiniaiinoueth e yia?aicaaeneiie aeeoioeyoeee EI, eioi?ay nii?iaiaeaeaaony aeoeaaoeeae aeuoaiey IO e auoiaeii I+ ec yoeo i?aaiaee. Noeioee?othuee yooaeo EI ia aeuoaiea IO (Vmax, iiieue I2/iei.ia aaeea) oiaiueoaaony a neaaeothuai ii?yaeea: Na2+ (35,71) > Sr2+ (33,90) > Cd2+ (26,32) > Mn2+ (22,02) >> La3+ (9,04).
Iieo/aiiue ?yae oanii ei??aee?oao n yioaeueieae aeae?aoaoeee EI (r =
-0,95) e einoaioaie onoie/eainoe eo eiiieaenia n aoeaoaoii (r = -0,90)
e ania?aaeiiaie eeneioie (r = -0,84). Neaaeiaaoaeueii, nei?inoue
yia?aicaaeneiie aeeoioeyoeee EI a IO ia/aie ii?aaeaeyaony yioaeueieae eo
aeae?aoaoeee e n?iaenoaii e NII- a?oiiai Na2+-oieii?oa?a IO.

Auyaeaiiue iaie eiaeae?othuee yooaeo EI ia i?ioiiioi?-noeioee?iaaiiia
aeuoaiea IO oaaee/eaaaony a ?yaeo Na2+, Sr2+, Mn2+ (ia aeeytho) < La3+ < Cd2+ . Aiaeiae/iue ?yae ia?acotho EI ii eo eiaeae?othuaio aeeyieth ia ADP-noeioee?iaaiiia aeuoaiea IO: Na2+, Sr2+ (ia aeeytho) < Mn2+ < La3+ < Cd2+. Iaa ?yaea oanii ei??aee?otho (r = 0,93-0,99) n eiinoaioaie onoie/eainoe eiiieaenia EI n SH- a?oiiaie oeenoaeia. Oaeei ia?acii, oaiaoaiea EI i?ioeannia ieeneaiey noano?aoia e ieeneeoaeueiiai oinoi?eee?iaaiey a IO ii?aaeaeyaony eo n?iaenoaii e SH- a?oiiai iae?iiieaeoe IO. EI eiaeae?otho o?ainii?o Na2+ a IO ii eiieo?aioiiio (Sr2+, Mn2+) eee niaoaiiiio (Cd2+) oeio. Eiaeaeoi?iua eiinoaiou (Ei , ieI) o?ainii?oa Na2+ a IO aeey EI aic?anoatho a ?yaeo: La3+ (2,11), Cd2+ (10,36), Mn2+ (49,29), Sr2+ (66,43). Iieo/aiiue ?yae oa?aeoa?ecoaony oaniie ei??aeyoeeae n yioaeueieae aeae?aoaoeee EI (r = -0,78) e eiinoaioaie onoie/eainoe eo eiiieaenia n aoeaoaoii (r = -0,96) e ania?aaeiiaie eeneioie (r = -0,91). Neaaeiaaoaeueii, a iaoaiecia o?ainii?oa Na2+ a iao?een IO e aai eiaeae?iaaiee eaoeiiaie ae?oaeo iaoaeeia aaaeiia ianoi caieiaao eo aeaaeae?aoaoeey e acaeiiaeaenoaea n ea?aieneeueiuie a?oiiaie Na2+-oieii?oa?a. Eaoeiiu Ca2+ e Cd2+, a ioee/ea io Sr2+, Mn2+ e La3+, eiaeooee?otho ia?aoiae aioo?aiiae iaia?aiu IO a ninoiyiea iiauoaiiie ianiaoeeoe/aneie i?iieoeaaiinoe. Eaoeiiu Sr2+ e Mn2+ niiniaiu eiieo?aioii oaiaoaoue yoio i?ioeann. Aianoa n oai iniaaiiinoe eeiaoeee Na2+- e Cd2+-eiaeooee?iaaiiiai iaaooaiey IO oeacuaatho ia ?acee/ea a iaoaieciao eiaeooee?iaaiey eie ia?aoiaea aioo?aiiae iaia?aiu IO a ninoiyiea iiauoaiiie ianiaoeeoe/aneie i?iieoeaaiinoe, /oi, aa?iyoii, iaoneiaeaii eo iaiaeeiaeiaui n?iaenoaii e NII- e SH-a?oiiai iae?iiieaeoe iaia?aiu IO. Neaaeiaaoaeueii, niiniaiinoue EI acaeiiaeaenoaiaaoue n ?acee/iuie ooieoeeiiaeueiuie nenoaiaie IO iaiaeeiaeiaa e ii?aaeaeyaony eo oeceei-oeie/aneeie naienoaaie, a /anoiinoe yioaeueieae aeae?aoaoeee e n?iaenoaii e eenei?iae- e na?iniaea?aeauei a?oiiai aeieeaaiaeia. Yoi iiaeao auoue eniieueciaaii aeey iai?aaeaiiiai iienea aioeaeioia e eaoeiiai ?aciuo iaoaeeia. Eeth/aaua neiaa: ieoioiiae?ee ia/aie, eaoeiiu iaoaeeia, aeuoaiea, ieeneeoaeueiia oinoi?eee?iaaiea, iiaeiuaiea eaeueoeey, ianiaoeeoe/aneay i?iieoeaaiinoue iaia?aiu. SUMMARY VOVKANYCH L.S. Effect of earthy and transient metals` cations on the functioning of rat liver mitochondria. - Manuscript. Thesis for scientific degree of the candidate of biological sciences by speciality 03.00.02 - Biophysics. - The Taras Shevchenko Kyiv University, Kyiv, 1999. The thesis are devoted to the investigation of the mechanisms of metals` cations (MC), including Ca2+, Sr2+, Mn2+, Cd2+, La3+, effect on the rat liver mitochondria (RLM) respiration, ADP-phosphorylation, Ca2+-accumulation and unspecific permeability of the RLM inner membrane. It has been shown, that RLM could accumulate MC in the energy-dependent process, velocity of which depends on their affinity to COO- groups of the RLM Ca2+-uniporter. Inhibition of the RLM substrate oxidation and oxidative phosphorylation by MC is correlated with the stability constants of MC with SH- groups of cysteine. MC inhibit RLM Ca2+ uptake in competitive (Sr2+, Mn2+ , La3+) and mixed (Cd2+) manner. The power of inhibition is determined by the MC hydratation enthalpy and their affinity to COO- groups of Ca2+-uniporter. Na2+ and Cd2+, but not Sr2+, Mn2+, La3+, induce RLM inner membrane unspecific permeability transition. Sr2+ and Mn2+ are competitive inhibitors of that process. Hence, ability of the MC to interact with the different RLM functional systems is uniqual and is determined by their physicochemical properties, especially by their hydratation enthalpy and affinity to oxygen- and sulphur-containing groups of bioligands. It could be used for the direct search of antidotes to different metals` cations. Key words: liver mitochondria, metals` cations, respiration, oxidative phosphorylation, calcium uptake, unspecific permeability of membrane.

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