Вплив антибіотиків і середовищ для культивування ооцитів in vitro на якісні показники сперми бугаїв: Автореф. дис… канд. с.-г. наук / П.М. Скляров,

OE?A?INUeEA AEAAeAI?ss AA?A?IEO IAOE

?INOEOOO OAA?EIIEOeOAA

Ia i?aaao ?oeiieno

Neey?ia Iaaei Ieeieaeiae/

OAeE 619.615.33:591.3591.463.1:636.22/.28

AIEEA AIOEA?IOEE?A ? NA?AAeIAEU AeEss

EOEUeOEAOAAIIss IIOeEO?A IN VITRO IA ssE?NI?

IIEACIEEE NIA?IE AOAA?A

Niaoe?aeuei?noue: 03.00.20. — a?ioaoiieia?y

Aaoi?aoa?ao

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe

Oa?e?a-1999

Aeena?oaoe??th ? ?oeiien

?iaioa aeeiiaia a eaai?aoi??? eoeueoeaoaaiiy ? o?ainieaioaoe?? aia??ii?a
?inoeoooo oaa?eiieoeoaa Oe?a?inueei? aeaaeai?? aa?a?ieo iaoe

Iaoeiaee ea??aiee aeieoi? a?ieia?/ieo iaoe, i?ioani?,

aieiaiee iaoeiaee ni?a?ia?oiee

a?aeae?eo a?ioaoiieia?? oa aaiii? ?iaeaia??? ?O OAAI

Aoa?ia Ieaen?e Aeieo?iae/

?inoeooo oaa?eiieoeoaa OAAI

Io?oe?ei? iiiiaioe:

1. Aeieoi? a?ieia?/ieo iaoe, i?ioani?, /eai-ei?aniiiaeaio OAAI,

Naoaoeueeee Ieeiea ?aaiiae/, aee?aeoi? ?inoeoooo ioao?aieoeoaa OAAI

2. Eaiaeeaeao a?ieia?/ieo iaoe, noa?oee iaoeiaee ni?a?ia?oiee,

E?oaeye Aiae??e Iao?iae/, caa?aeoaa/ eaai?aoi??? ?icaaaeaiiy
/a?aiii-?yai? ooaeiae oa aaiioiiaeo ii??ae ?inoeoooo ?icaaaeaiiy ?
aaiaoeee oaa?ei OAAI

I?ia?aeia onoaiiaa — Aea?aeaaiee iaoeiai-aeine?aeiee eiio?ieueiee
?inoeooo aaoa?eia?ieo i?aia?ao?a ? ei?iiaeo aeiaaaie, i. Euea?a

Caoeno aeena?oaoe?? a?aeaoaeaoueny 23 aa?aciy 1999 ?ieo i 1000 aiaeei?
ia can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae.65.356.02 i?e ?inoeooo?
oaa?eiieoeoaa OAAI (312120, Oa?e?anueea iaeanoue, Oa?e?anueeee ?aeii,
i/a Eoeeie/?)

C aeena?oaoe??th iiaeia iciaeiieoenue a a?ae?ioaoe? ?inoeoooo
oaa?eiieoeoaa OAAI

Aaoi?aoa?ao ?ic?neaiee 16 ethoiai 1999 ?ieo

A/aiee nae?aoa? niaoe?ae?ciaaii?

a/aii? ?aaee, eaiaeeaeao a?ieia?/ieo iaoe Ni?ao? O.I.

1

Caaaeueia oa?aeoa?enoeea ?iaioe

Aeooaeuei?noue oaie. Nia?ia aoaa?a aeei?enoiao?oueny aeey ooo/iiai
in?iai?iiy ? caie?aeiaiiy in vitro. A inoaiiueiio aeiaaeeo aeey ??
iiia?aaeiuei? ia?iaee (eaiaoeeoaoe??) ? eoeueoeaoaaiiy c iioeeoaie
canoiniaothoue ??ci? na?aaeiaeua ? aeiaaaee. Iaeiae, i?e oeueiio
i?aeoe/ii ia aeine?aeaeaiei caeeoa?oueny ieoaiiy i?i aieea oeeo
na?aaeiaeu oa aeiaaaie ia oaeee iieaciee nia?ie, ye aeaeeaai?noue.

I?e ooo/iiio in?iai?ii? aaaeeeaei ? oaea ieoaiiy, ye naiaoe?y nia?ie
aoaa?a-ie?aeiee?a. Aeooaeuei?noue eiai iia’ycaia c oei, ui ?nioth/?
iaoiaee iaea?aeaiiy oa caa??aaiiy nia?ie ia caaacia/othoue a on?o
aeiaaeeao a?eueii? a?ae i?e?ii?aai?ci?a nia?ie. Naiaoe?y i?e oeueiio ia
caaaeaee i?eaiaeeoue aei aaaeaieo ?acoeueoao?a a ca’yceo c oei, ui
aeaye? i?aia?aoe aai ia aoaeoeai? ii a?aeiioaiith aei i?e?ioei?e nia?ie,
aai ? oiene/ieie aei nia?i??a.

Iaoa aeine?aeaeaiue. Iaoith aeine?aeaeaiue aoei aea/aiiy aieeao ia
ye?ni? iieacieee nia?ie aoaa?a neioaoe/ieo na?aaeiaeu ? aeiaaaie, ye?
aeei?enoiaothoueny aeey eoeueoeaoaaiiy iioeeo?a in vitro, a oaeiae
?ic?iaea aoaeoeaiiai aioeaaeoa??aeueiiai i?aia?aoo aeey naiaoe?? nia?ie
aoaa?a-ie?aeiee?a.

A caaaeaiiy aeine?aeaeaiue aoiaeeei:

1. Anoaiiaeaiiy aieeao ia aeaeeaai?noue nia?i??a na?aaeiaeu ? aeiaaaie
aeey eoeueoeaoaaiiy iioeeo?a.

2.Aecia/aiiy aeaeeaaiino? nia?i??a a i?ioean? eaiaoeeoaoe??.

3. Aea/aiiy aieeao ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia
?acoeueoaoeai?noue e??ieiina?aaoe??.

4. Aecia/aiiy aieeao aaioai?oeeio oa aii?oeee?io ia aeaeeaai?noue
iaoeaii? nia?ie aoaa?a.

5. Aecia/aiiy aeaeeaaiino? ? caie?aeithaaeueii? caeaoiino? nia?i??a i?e
aeiaeaaaii? aioea?ioee?a ia?aae caii?iaeoaaiiyi ? i?e ?icii?iaeoaaii?.

6. ?ic?iaea aoaeoeaiiai aioei?e?iaiiai i?aia?aoo aeey naiaoe?? nia?ie
aoaa?a.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aia?oa aeaia a?ieia?/ia
ii??aiyeueia ioe?iea na?aaeiaeu ? aeiaaaie, ye? aeei?enoiaothoueny aeey
eoeueoeaoaaiiy iioeeo?a iica i?aai?ciii. Anoaiiaeaii iiceoeaiee aieea
na?aaeiaeu (TALP, BWW, A??inoa?a, ONI-199, E?aan-??iaa?a) ? aeiaaaie
(ai?oae?th iaoee ? yeoeaaiae?a, oie?eoey?ii? ??aeeie, oaoaeueii?
ne?iaaoee, aaia?eio, eioa?io), ye? aeei?enoiaothoueny aeey
eoeueoeaoaaiiy iioeeo?a ? aia??ii?a in vitro ia aeaeeaai?noue iaoeaii? ?
e??ieiina?aiaaii? nia?ie aoaa?a. Iaea?eueo aoaeoeaiei aoei na?aaeiaeua
TALP, a c aeiaaaie — ai?oae?e yeoeaaiaeo a eiioeaio?aoe?? 25%.
Aeieacaii, ui i?ioean ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia
/eieoue iaaaoeaiiai aieeao ia aeaeeaai?noue nia?i??a ia?aae ? i?ney
e??ieiina?aaoe??, a oaeiae ia ?o caie?aeithaaeueio caeaoi?noue.

Anoaiiaeaii iiceoeaiee aieea eiiieaeno aioea?ioee?a aaioai?oeeio ?
aii?oeee?io ia aeaeeaai?noue na?aei?icaaaeaii? ? caii?iaeaii-a?aeoaiooi?
nia?ie aoaa?a. Aecia/aii iioeiaeueio eiioeaio?aoe?th aaioai?oeeio — 25
iea/ie ? aii?oeee?io — 100 IAe, ye? cieaeothoue i?e?iaio eiioai?iaoe?th
nia?ie aoaa?a c? caa?aaeaiiyi ?? caie?aeithaaeueii? caeaoiino?.

?ic?iaeaii aioei?e?iaia na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie aoaa?a o
a?aee?eoeo a?aioeao ? niin?a o?eaaeiai eiai caa??aaiiy a ??aeeiio acio?.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a. I?iaaaeaii ii??aiyeueia
aea/aiiy aeaeeaaiino? nia?i??a aoaa?a i?e aeei?enoaii? na?aaeiaeu ?
aeiaaaie aeey eoeueoeaoaaiiy in vitro, a oaeiae aea/aii aeaeeaai?noue
nia?i??a o i?ioean? ia?iaee ?o ia?aae caie?aeiaiiyi in vitro ? aecia/aii
aieea oeueiai i?ioeano ia ?acoeueoaoeai?noue e??ieiina?aaoe??.

?ic?iaeaii i?aia?ao aeey naiaoe?? nia?ie aoaa?a, yeee caaacia/o?
i?aeaeuaiiy caie?aeiaiino? nia?ie ia 11-18 % ? ??aaiue i?e?iaii?
caa?oaeiaiino? o a?aeiia?aeiino? aei aeiia aea?aenoaiaea?oo aeey nia?ie,
caii?iaeaii? o a?aee?eoeo a?aioeao. E??i oeueiai, i?aeoe/ia cia/aiiy
i?aia?aoo iieyaa? a oiio, ui a?i ia? aioei?e?iaio aeoeai?noue i?ioyaii
o?eaaeiai /ano i?e caa??aaii? a ??aeeiio acio? ? caaacia/o? naiaoe?th
nia?ie aaciina?aaeiuei ia?aae aaaaeaiiyi ?? a noaoaa? oeyoe naiee.

Iniaenoee aianie caeiaoaa/a. A?eueo?noue aeine?aeaeaiue aeeiiaii aaoi?ii
naiino?eii. Iaoeiai-ae?iaie/? aeine?aee i?iaaaeaii ca o/anoth aaoi?a.
Iaoiaee/i? i?aeoiaee oa noaie aeine?ae?a a?aei?aoeueiaaii ni?eueii c
iaoeiaei ea??aieeii.

Ai?iaaaeaeaiiy ?acoeueoao?a aeine?aeaeaiue. ?acoeueoaoe aeine?aeaeaiue
ai?iaaaeaeaii a ae/a “Eoooc?aea”, a?aeae?eeo “O?oica” ae/a “Oe?a?iea” oa
aea?aeieaicaaiae? “*a?aiiee Aaeaoaiue” Oa?e?anueei? iaeano?.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??. ?acoeueoaoe aeine?aeaeaiue aoee
aeeeaaeai?, iaaiai?ai? ? io?eiaee iiceoeaio ioe?ieo ia a/aieo ?aaeao
?inoeoooo oaa?eiieoeoaa OAAI, a oaeiae ia I?aeia?iaei?e
iaoeiai-i?aeoe/i?e eiioa?aioe??, i?enay/ai?e 100-??//th a?ae aeiy
ia?iaeaeaiiy ssoeaiea I.A. (Oa?e?a, 1997?.); I?aeia?iaei?e
iaoeiai-ae?iaie/?e eiioa?aioe?? “Aeei?enoaiiy o?ainieaioaoe?? aia??ii?a
a naeaeoe?? ? a?aeoai?aii? n?euenueeiainiiaea?nueeeo oaa?ei”
(Aneai?y-Iiaa, 1997 ?.); I?aeia?iaei?e iaoeiai-i?aeoe/i?e eiioa?aioe??,
i?enay/ai?e 100-??//th a?ae aeiy ia?iaeaeaiiy N.C. Aaeeoeueeiai “No/ani?
i?iaeaie a?ieia??, aaoa?eia?ii? iaaeeoeeie, cii?iaeaia??? oa oaoiieia??
i?iaeoeo?a oaa?eiieoeoaa” (Euea?a, 1997?.); I?aeia?iaei?e eiioa?aioe??,
i?enay/ai?e 70-??//th a?ae aeiy ia?iaeaeaiiy O.?. Inoaoei (Oa?e?a,
1998?.); eiioa?aioe?? iieiaeeo a/aieo ?O OAAI (Oa?e?a, 1998 ?.);
I?aeia?iaei?e eiioa?aioe?? “Aeei?enoaiiy no/anieo iieaeoey?ii-aaiaoe/ieo
? a?ioaoiieia?/ieo ?ic?iaie o aaiaoeei-naeaeoe?eieo aeine?aeaeaiiyo”
(Iaeana, 1998 ?.); I?aeia?iaei?e iaoeia?e eiioa?aioe?? “No?aoaaey e
oaeoeea ai?ueau n eioaeoeeiiiuie aieaciyie” (Oa?e?a, 1998 ?.).

Ianya oa no?oeoo?a aeena?oaoe??. Aeena?oaoe?y aeeeaaeaia ia 186
noi??ieao iaoeiiieniiai oaenoo ? neeaaea?oueny c anooio, iaeyaeo
e?oa?aoo?e, iaoa??ae?a oa iaoiae?a aeine?aeaeaiue, aeniiae?a oa
i?iiiceoe?e ae?iaieoeoao. I?noeoue 38 oaaeeoeue, 1 ?enoiie oa 11
aeiaeaoe?a. Nienie e?oa?aoo?e aeeth/a? 352 iaeiaioaaiiy, o oiio /ene?
179 ?iicaiiith iiaith.

Iaoa??aee oa iaoiaeeea aeine?aeaeaiue

Eaai?aoi?i? aeine?aeaeaiiy i?iaiaeeeeny a a?aeae?e? a?ioaoiieia?? ?
aaiii? ?iaeaia??? ?inoeoooo oaa?eiieoeoaa OAAI ? Oa?e?anuee?e
iaenaiai?aenoaioe??; iaoeiai-ae?iaie/? aeine?aee — a a?aeae?eeo “O?oica”
ae/a “Oe?a?iea”, ae/a “Eoooc?aea” ? AeIC “*a?aiiee Aaeaoaiue”
Oa?e?anueei? iae.

Iaoa??aeii aeey aeine?aeaeaiue aoea iaoeaia nia?ia, iaea?aeaia a?ae
aoaa?a-ie?aeiee?a, ye? oo?eioaaeeny ia noaioe?? ooo/iiai in?iai?iiy ae/a
“Oe?a?iea”, ? caii?iaeaii-a?aeoaiooa nia?ia c e??iaaieo a?aeae?eo
a?ioaoiieia?? ? aaiii? ?iaeaia???.

O aeine?aeao aeei?enoiaoaaee na?aaeiaeua TALP, BWW, A??inoa?a, ONI-199,
E?aan-??iaa?a ? aeiaaaee — aaia?ei, eioa?i, ai?oae?e iaoee ? yeoeaaiaeo,
oie?eoey?io ??aeeio, oaoaeueio ne?iaaoeo.

Eaiaoeeoaoe?th nia?i??a i?iaiaeeee oeyoii ?ieoaaoe?? c aaia?eiii oa
oeaio?eooaoaaiiy ca iaoiaeeeith J.J. Parrish et al. (1986).

Caii?iaeoaaiiy nia?ie a iaeeoeueiaaieo oa a?aee?eoeo a?aioeao i?iaiaeeee
ca Oa?e?anueeith oaoiieia??th ? ia ooi?iieanoia?e ieanoei? ca
caaaeueiii?eeiyoith iaoiaeeeith c ?ic?aooieo ia iaio 15 iei. aeoeaieo
nia?i??a a aeic? i?ney ?icii?iaeoaaiiy.

Na?aaeiaeua c aioea?ioeeaie aiooaaee c ?ic?aooieo 12, 25, 50 /e 100 iea
aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie na?aaeiaeua, aeey /iai
aeei?enoiaoaaee 4%-iee ?ic/ei aaioai?oeeio noeueoaoo oa aii?oeee?io
iao???ao n?eue. Nia?iinai-3 aeiaeaaaee c ?ic?aooieo 50 oen. IAe ia 100
ie na?aaeiaeua.

Naioth/a na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie i?ney i?eaiooaaiiy
?icoaniaoaaee a iie?aoeeaiia? aiioee ii 1 ie, aa?iaoe/ii oiaeiaoaaee,
caii?iaeoaaee oeyoii caio?aiiy a ??aeeee acio, a yeiio caa??aaee aei
aeei?enoaiiy. I?e aeaeiina?aaoe?? nia?ie iiia?aaeiuei ?icii?iaeoaaee
na?aaeiaeua ia aiaeyi?e aai? i?e t=40°N i?ioyaii 5 oa., a iio?i
aeei?enoiaoaaee eiai ye cae/aeiee 2,9 %-iee ?ic/ei oeeo?aoo iao??th.

Aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? nia?ie aecia/aee ca
A.E. I?eiaaiiaei (1962):
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,

aea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 — iieaciee ii/aoeiai? aeoeaiino? nia?i??a;
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- noia
iieaciee?a aeoeaiino? /a?ac eiaeio aiaeeio.

Eiao?oe??io oiene/iino? (iaoe?aeeeaino?) ?ic?aoiaoaaee ca oi?ioeith,
cai?iiiiiaaiith I.A. Aaeaoiaei (1972):

picscalex162010009000003540000000000120000000000050000000902000000000400
000002010100050000000102ffffff00040000002e011800050000003102010000000500
00000b0200000000050000000c02200220011200000026060f001a00ffffffff00001000
0000c0ffffffa6ffffffe0000000c60100000b00000026060f000c004d61746854797065
000050000a00000026060f000a00ffffffff010000000000030000000000picscalex203
010009000003530100000300170000000000050000000902000000000400000002010100
050000000102ffffff00040000002e01180005000000310201000000050000000b020000
0000050000000c02400460061200000026060f001a00ffffffff000010000000c0ffffff
bdffffff20060000fd0300000b00000026060f000c004d617468547970650000f0000900
0000fa02000010000000000000002200040000002d0100000500000014020002f2030500
0000130200028a0517000000fb0280fe0000000000009001000000cc0402001054696d65
73204e657720526f6d616e2043797200cca0040000002d01010008000000320a6002ba05
010000002c0017000000fb0220ff0000000000009001000000cc0402001054696d657320
4e657720526f6d616e2043797200cca0040000002d01020004000000f001010008000000
320aeb03d90401000000320008000000320ac901d50401000000310017000000fb0280fe
0000000000009001010000cc0402001054696d6573204e657720526f6d616e2043797200
cca0040000002d01010004000000f001020008000000320a8b030c0401000000e0000800
0000320a6901210401000000610008000000320a6002460001000000ca0017000000fb02
20ff0000000000009001010000cc0402001054696d6573204e657720526f6d616e204379
7200cca0040000002d01020004000000f001010008000000320ac0025a0101000000d200
10000000fb0280fe0000000000009001000000020002001053796d626f6c000204000000
2d01010004000000f001020008000000320a60027d02010000003d000a00000026060f00
0a00ffffffff01000000000010000000fb021000070000000000bc02000000cc01020222
53797374656d00cc040000002d01020004000000f0010100030000000000

aea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 — na?aaei? /enei ?ooeeaeo nia?i??a o aeo?aei?e
nia?i?;
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 — na?aaei? /enei ?ooeeaeo nia?i??a /a?ac 3 aiaeeie
i?ney ?icaaaeaiiy nia?ie na?aaeiaeuai c i?aia?aoii.

Caie?aeithth/o caeaoi?noue nia?i??a aecia/aee ca a?aenooiino? ianooiieo
noaoaaeo oeeee?a i?ioyaii 2-o i?nyoe?a ? ca aeaieie ?aeoaeueiiai
aeine?aeaeaiiy ia o?euei?noue i?ney in?iai?iiy /a?ac 3 i?nyoe?.
Caie?aeiai?noue aecia/aee ca a?aenioeii o?eueieo a?ae caaaeueii?
e?eueeino? ia?aeiii in?iaiaieo ei??a. A aeine?ae? ? eiio?ie?
aeei?enoiaoaaee ee?i?/ii caei?iaeo oaa?ei ca i?eioeeiii a?oi-aiaeia?a ca
ii?iaeith, a?eii ? /anii i?iyao ia?oiai noaoaaiai oeeeeo i?ney ioaeaiiy.
Aeyaeaiiy ei??a a ioio? i?iaiaeeee aea?/? ia aeiao (a?aioe? oa aaa/a??).
Nia?io aaiaeeee iaii- ? ?aeoi-oea?a?eaeueiei niiniaaie ca?aeii ae?th/i?
?ino?oeoe??.

A?aea?? i?ia oa aaeoa??ieia?/i? aeine?aeaeaiiy i?iaiaeeee a?aeiia?aeii
aei aea?aenoaiaea?o?a 20909.1-75 oa 20909.2-75.

Noaoenoe/io ia?iaeo ?acoeueoao?a i?iaiaeeee ca aeiiiiiaith
ia?ni-iaeueiiai eiii’thoa?a Pentium-120 ca?aeii i?ia?aiiiai
caaacia/aiiy: WORD 7.0, EXEL 7.0, MICROSOFT OFFICE o??ie “MICROSOFT”
(Ieaei E.A., Ieioeineee I.A., 1970, Eaeei A.O., 1990).

?acoeueoaoe aeine?aeaeaiue

A ia?o?e na??? aeine?ae?a ie aea/aee aieea ia aeaeeaai?noue nia?i??a
na?aaeiaeu oa aeiaaaie aeey eoeueoeaoaaiiy in vitro iioeeo?a.
?acoeueoaoe aeine?aeaeaiue i?eaaaeaii a oaae. 1.

Oaaeeoey 1

Aeaeeaai?noue nia?i??a a na?aaeiaeuao aeey eoeueoeaoaaiiy in vitro

Na?aaeiaeuaIaoeaia nia?iaCaii?iaeaii-a?aeoaiooa
nia?iaeiio?ieueaeine?aeeiio?ieueaeine?ae1.TALP:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,3±0,40

42,3±2,1

12

11,4±0,26 **

49,8±2,2 *

12

6,1±0,24

19,9±0,9

12

8,1±0,24 **

23,5±1,0

122. BWW:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,8±0,22

43,6±1,3

15

10,5±0,30 **

46,4±1,3

15

6,1±0,27

20,8±0,9

10

7,7±0,15 **

24,1±0,7

103. A??inoa?a:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

9,1±0,23

43,2±1,4

12

10,8±0,27 **

46,3±1,2

12

6,1±0,24

18,9±0,5

11

6,6±0,26 *

21,2±0,6

114. ON-199:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

9,9±0,22

47,1±1,1

11

11,0±0,36

50,8±1,4

11

5,9±0,26

26,1±1,3

9

7,1±0,26 *

29,3±1,2

95. E?aan-??iaa?a:

a) aeaeeaai?noue, aiae.

a) aaniethoiee iieaciee aeaeeaaiino?, iae.

n

8,3±0,35

43,2±1,6

12

8,8±0,36

44,4±1,5

12

6,2±0,24

24,4±0,5

12

6,5±0,26

24,9±0,6

12* — P<0,01; ** - P<0,001 A oiae? aeine?aeaeaiue aoei anoaiiaeaii, ui a aeine?aeiiio na?aaeiaeu? TALP a ii??aiyii? c eiio?ieai aeaeeaai?noue i?aeaeuoaaeanue aeey caii?iaeaii-a?aeoaiooi? ? iaoeaii? nia?ie ia 2,0 ? 3,1 aiae. (?<0,001), a aaniethoiee iieaciee aeaeeaaiino? ia 3,6 ? 7,5 iae. (?<0,01), a?aeiia?aeii. A na?aaeiaeu? BWW aeine?aei? iieacieee aoee aeua eiio?ieueieo ia 1,7 aiae. (?<0,001) ? 1,6 aiae. - aeey iaoeaii? oa 2,8 iae. (?<0,001) ? 3,3 iae. - aeey caii?iaeaii-a?aeoaiooi? nia?ie. Aeine?aee c na?aaeiaeuai A??inoa?a iieacaee, ui a ii??aiyii? c eiio?ieai aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? i?aeaeuoaaeeny ia 1,7 aiae. (?<0,001) ? 3,1 iae. - aeey iaoeaii? ? 0,5 aiae. (?<0,001) ? 2,3 iae. - aeey caii?iaeaii-a?aeoaiooi? nia?ie. A na?aaeiaeu? ON-199 oe? iieacieee aoee aeua eiio?ieueieo ia 1,1 aiae., 3,7 iae. oa 1,2 aiae. (?<0,01), 2,4 iae., a?aeiia?aeii aeey iaoeaii? oa caii?iaeaii-a?aeoaiooi? nia?ie. A na?aaeiaeu? E?aan-??iaa?a iieacieee aeaeeaaiino? nia?i??a a ii??aiyii? c eiio?ieai ca?eueooaaeeny ia 0,5 aiae., 1,2 iae. - aeey iaoeaii? ? ia 0,3 aiae., 0,5 iae. - aeey caii?iaeaii-a?aeoaiooi? nia?ie. A ?acoeueoao? aeine?aeaeaiue aoei anoaiiaeaii, ui ia?aa??ai? na?aaeiaeua aeey eoeueoeaoaaiiy in vitro iiceoeaii aieeaaee ia aeaeeaai?noue nia?i??a aoaa?a. Iieacieee aeaeeaaiino? a na?aaeiaeuao iia??ooaaeeny a oae?e iine?aeiaiino?: 1) TALP, 2) A??inoa?a, 3) BWW, 4) ONI-199 ? 5) E?aan-??iaa?a. A ianooii?e na??? aeine?ae?a ie aea/aee aieea ??cieo aeiaaaie aeey eoeueoeaoaaiiy nia?i??a ? iioeeo?a ia aeaeeaai?noue nia?i??a aoaa?a. ?acoeueoaoe i?eaaaeaii a oaae. 2. I?e aeei?enoaii? aaia?eio aoei anoaiiaeaii, ui a?i o an?o aeine?aeaeoaaieo eiioeaio?aoe?yo i?aeaeuoaaa aeaeeaai?noue nia?i??a a ii??aiyii? c eiio?ieai. Iaee?au? ?acoeueoaoe aoei iaea?aeaii i?e aeiaeaaaii? 100 IAe aaia?eio. I?e oeueiio aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? ia?aaeuoaaee eiio?ieue ia 1,8 aiae. (?<0,001), 5,9 iae. (?<0,01) - aeey iaoeaii? ? ia 1,6 aiae. (?<0,01), 2,0 iae. (?<0,01) - aeey caii?iaeaii-a?aeoaiooi? nia?ie. Iaea?eueo aoaeoeaiith eiioeaio?aoe??th eioa?io aeyaeeiny aeiaeaaaiiy eiai a aeic? 600 iea. I?e oeueiio aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? ia?aaeuoaaee eiio?ieue ia 1,5 aiae. (?<0,01), 4,8 iae. (?<0,01) oa 0,7 aiae. (?<0,01), 1,6 iae. (?<0,01), a?aeiia?aeii aeey iaoeaii? ? caii?iaeaii-a?aeoaiooi? nia?ie. Ai?oae?e iaoee iaea?eueo aoaeoeaiee aoa o 25% eiioeaio?aoe??, eiee aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? a aeine?ae? aoee e?aua eiio?ieueieo ia 0,8 aiae., 3,0 iae. ? 0,5 aiae. (?<0,01), 1,6 iae., a?aeiia?aeii. I?e aeiaeaaaii? ai?oae?aeueieo ee?oei yeoeaaiaeo iaee?au? iieacieee aeaeeaaiino? niinoa??aaeeny i?e aeiaeaaaii? ?o a eiioeaio?aoe?? 25 %, aeine?aei? iieacieee aoee aeua eiio?ieth ia 2,0 aiae. (?<0,001), a aaniethoiee iieaciee aeaeeaaiino? - 3,4 iae. -- a?aeiia?aeii aeey iaoeaii? ? ia 1,8 aiae. (?<0,01), 2,8 iae. - aeey caii?iaeaii-a?aeoaiooi? nia?ie. Iaee?au? ?acoeueoaoe i?e aeei?enoaii? oie?eoey?ii? ??aeeie aoei iaea?aeaii i?e aeiaeaaaii? ?? a eiioeaio?aoe?? 25%, eiee a aeine?ae? aeaeeaai?noue ? aaniethoiee iieaciee aeaeeaaiino? aoee aeua eiio?ieth ia 2 aiae. (?<0,01), 3,5 iae. oa 0,6 aiae. (?<0,01), 1,8 iae. - a?aeiia?aeii, aeey iaoeaii? ? caii?iaeaii-a?aeoaiooi? nia?ie. Oaoaeueia ne?iaaoea iaea?eueo aoaeoeaiith aoea i?e aeiaeaaaii? ?? a 10% eiioeaio?aoe??, a oeueiio aeiaaeeo aeine?aei? iieacieee aeaeeaaiino? aoee aeua eiio?ieueieo ia 1,8 aiae. (?<0,001), aaniethoiee iieaciee aeaeeaaiino? - 9,9 iae. (?<0,001) oa ia 1,1 aiae. (?<0,01), 3,2 iae. (?<0,01) – a?aeiia?aeii aeey iaoeaii? ? caii?iaeaii-a?aeoaiooi? nia?ie. Oaaeeoey 2 Aeaeeaai?noue nia?i??a i?e aeei?enoaii? aeiaaaie (ia 1 ie oeeo?aoo iao??th) AeiaaaeeIaoeaia nia?iaCaii?iaeaii-a?aeoaiooa nia?ianAeaeeaai?noue nia?i??a, aiae.Aaniethoiee iieaciee aeaeeaaiino?, iae.nAeaeeaai?noue nia?i??a, aiae.Aaniethoiee iieaciee aeaeeaaiino?, iae.1. Aaia?ei, IAe: a) eiio?ieue a) 50 a) 100 a) 15016 8,5±0,30 9,5±0,26 * 10,3±0,24 ** 10,2±0,25 ** 35,7±1,3 38,8±1,2 41,6±1,1 * 40,9±1,0 *14 6,1±0,25 7,2±0,22 * 7,7±0,19 * 7,5±0,20 ** 25,9±0,8 26,3±0,9 * 27,9±0,7 * 26,8±0,8 *2. Eioa?i, iea: a) eiio?ieue a) 200 a) 400 a) 60010 8,3±0,33 9,5±0,27 * 9,8±0,20 * 9,9±0,28 * 36,9±1,6 39,4±1,2 41,7±1,1 * 41,0±1,1 *7 5,8±0,27 5,9±0,24 * 6,5±0,21 * 7,0±0,27 * 26,2±1,5 27,1±1,4 * 27,8±1,1 * 28,9±1,2 *3. Ai?oae?aeuei? ee?oeie iaoee, %: a) eiio?ieue a) 50 a) 25 a) 10 9 9,7±0,37 10,3±0,24 * 10,5±0,34 10,1±0,20 41,7±1,0 43,4±1,0 44,7±1,0 * 43,8±1,09 6,1±0,24 6,5±0,26 * 6,6±0,26 * 6,3±0,22 24,3±0,5 25,5±0,6 * 25,9±0,6 * 24,9±0,74. Ai?oae?aeuei? ee?oeie yeoeaaiaeo, %: a) eiio?ieue a) 50 a) 25 a) 10 15 8,2±0,29 10,2±0,18 ** 10,2±0,20 ** 9,4±0,16 * 38,3±1,3 41,9±1,3 41,7±1,5 39,4±1,512 6,1±0,26 7,8±0,21 ** 7,9±0,23 * 6,9±0,21 * 21,5±0,9 24,1±1,0 24,3±1,0 23,5±1,15. Oie?eoey?ia ??aeeia, %: a) eiio?ieue a) 50 a) 25 a) 10 13 8,1±0,22 9,3±0,20 * 9,3±0,20 * 8,5±0,29 39,8±1,3 43,2±1,2 43,3±1,7 40,9±1,410 6,1±0,25 6,9±0,24 * 6,7±0,25 * 6,4±0,26 * 24,5±0,6 26,9±0,5 26,3±0,8 25,8±0,96. Oaoaeueia ne?iaaoea, %: a) eiio?ieue a) 50 a) 25 a) 10 16 8,4±0,23 9,3±0,22 ** 10,1±0,22 ** 10,2±0,22 * 36,7±1,2 42,3±1,0 ** 46,0±1,2 ** 46,6±1,3 **14 6,0±0,25 6,2±0,26 ** 6,9±0,25 * 7,1±0,24 * 26,2±1,3 27,7±1,4 * 28,9±1,2 * 29,5±1,2 ** - P<0,01; ** - P<0,001 Aeine?aee c aeei?enoaiiyi aeiaaaie iieacaee, ui aeiaaaee, ye ? na?aaeiaeua, ia aieeaathoue iaaaoeaii ia aeaeeaai?noue nia?i??a aoaa?a, a iaaiaee, a o?e /e ?io?e i??? iie?auothoue ?o. Oea na?ae/eoue i?i oa, ui aeiaaaee ni?eyoeeai ae?thoue ia iai?i ?a/iaei nia?i??a ? ?o o?c?ieia?/iee noai. A ianooii?e na??? aenia?eiaio?a ie aea/aee aieea i?ioeano ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro (eaiaoeeoaoe??) ia aeaeeaai?noue nia?ie aoaa?a. ?acoeueoaoe aeine?aeaeaiue i?eaaaeaii a oaae. 3. Oaaeeoey 3 Aeaeeaai?noue nia?i??a aoaa?a a i?ioean? eaiaoeeoaoe?? Nia?ianAeaeeaai?noue nia?i??a, aiae.Aaniethoiee iieaciee aeaeeaaiino?, iae.1. Iaoeaia nia?ia: a) eiio?ieue a) i?ney ?ieoaaoe?? c aaia?eiii a) i?ney oeaio?eooaoaaiiy12 10,4±0,26 11,6±0,26 * 11,1±0,23 * 49,6±4,8 57,4±4,3 54,6±4,42. Caii?iaeaii-a?aeoaiooa nia?ia: a) eiio?ieue a) i?ney ?ieoaaoe?? c aaia?eiii a) i?ney oeaio?eooaoaaiiy8 6,2±0,17 7,1±0,19 * 6,9±0,19 * 17,6±0,9 20,2±0,8 * 19,6±0,9* - P<0,01 A oiae? aeine?aeo aoei anoaiiaeaii, ui iaee?au? iieacieee aoei iaea?aeaii i?ney ?ieoaaoe?? nia?i??a c aaia?eiii, a i?ney oeaio?eooaoaaiiy niinoa??aaeany oaiaeaioe?y aei cieaeaiiy aeaeeaaiino?. Iaeiae a ii??aiyii? c eiio?ieai aeine?aei? iieacieee aeaeeaaiino? aoee e?aueie a on?o aeiaaeeao. Oiaoi i?ioean ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia aieeaaa iaaaoeaii ia aeaeeaai?noue nia?i??a. O ianooiieo aenia?eiaioao aea/aee ae?th oeueiai ae i?ioeano ia ?acoeueoaoeai?noue e??ieiina?aaoe?? ? in?iai?iiy. ?acoeueoaoe aeine?aeo i?eaaaeaii a oaae. 4. Oaaeeoey 4 Aeaeeaai?noue oa caie?aeiai?noue eaiaoeeoiaaieo nia?i??a aoaa?a i?ney caii?iaeoaaiiy-a?aeoaaaiiy IieacieeeEiio?ieueAeine?aeAeaeeaai?noue nia?i??a, aiae. Aaniethoiee iieaciee aeaeeaaiino?, iae. n5,9±0,29 21,6±0,8 156,1±0,28 22,5±0,7 15In?iaiaii O?eueieo %20 9 45±11,423 10 43±10,5Aoei anoaiiaeaii, ui a eiio?ie? aeaeeaai?noue nia?i??a aoea ieae/a, i?ae a aeine?ae? ia 0,2 aiae., aaniethoiee iieaciee aeaeeaaiino? oaeiae ieae/a ia 0,9 iae. I?e in?iai?ii? ei??a oe??th nia?iith caie?aeiai?noue neeaea 45%- a eiio?ie? ? 43% - a aeine?ae?. Iaea?aeai? aeai? na?ae/aoue i?i oa, ui nia?ia i?ney i?ioeano eaiaoeeoaoe?? ? caii?iaeoaaiiy-a?aeoaaaiiy caa??aa? ia o?i aea ??ai? caie?aeithaaeueio caeaoi?noue, oiaoi ia?iaea nia?i??a ia?aae caie?aeiaiiyi in vitro ia aieeaaea iaaaoeaii ia ?acoeueoaoeai?noue e??ieiina?aaoe??.. Ae?oaa /anoeia iaoi? ?iaioe aoea i?enay/aia aea/aiith aeaeeaaiino? ? caie?aeithaaeueii? caeaoiino? nia?i??a, a oaeiae i?e?iaii? caa?oaeiaiino? i?e aeiaeaaaii? aioea?ioee?a aii?oeee?io ? aaioai?oeeio aei iaoeaii? nia?ie, ia?aae ?? caii?iaeoaaiiyi ? i?e ?icii?iaeoaaii?. Nii/aoeo iaie aoei aecia/aii ?acenoia?aio aei iaea?eueo ?iciianthaeaeaieo aioea?ioee?a: ienaoeee?io, ea?aai?oeee?io, aaiceeiai?oeee?io, iiiii?oeeio, iie?i?eneio, a?eo?ii?oeeio, ieaaiaeii?oeeio, no?aioii?oeeio, eaiai?oeeio, ?eoaii?oeeio, eaaii?oeaoeio, oao?aoeeee?io, e?ieii?oeeio, ooceae?io, oeaoae?eneio, aeieneoeeee?io oa iaii?oeeio. C oeeo aioea?ioee?a a nai?o aeine?aeaeaiiyo ie aeei?enoiaoaaee aii?oeee?i ? aaioai?oeei, oae ye naia aei ieo iaea?eueo /ooeeaith aoea i?e?ioei?a ? aei oiai ae oea aeineoue ?iciianthaeaeai? ? a?eueo aeinooii? aioea?ioeee, ye? a eiia?iaoe?? i?ae niaith iathoue oe?ieee niaeo? aioei?e?iaii?, aaeoa?eoeeaeii? oa aaeoa??inoaoe/ii? ae?? o a?aeiioaii? a?ai-iiceoeaieo ? a?ai-iaaaoeaieo aaeoa??e. Iao? iiaeaeueo? aeine?aeaeaiiy aoei i?enay/aii aeai?o iioeiaeueii? eiioeaio?aoe?? aioea?ioee?a i?e caii?iaeoaaii? nia?ie. Nii/aoeo aecia/aee aieea aii?oeee?io ? aaioai?oeeio ia aeaeeaai?noue nia?i??a ?c iaoeaii? nia?ie. Aoei anoaiiaeaii, ui iioeiaeueiei aoei aeiaeaaaii? 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie oeeo?aoo iao??th, eiee iieacieee aeaeeaaiino? aoee aeu? ia o?eueee eiio?ieueieo, a ? a ii??aiyii? c ae?oaeie aeine?aeieie eiioeaio?aoe?yie. Iiaeaeueo? aeine?aeaeaiiy aoee ni?yiiaai? ia iiooe iioeiaeueii? aeice aeacaieo aioea?ioee?a i?e e??ieiina?aaoe?? nia?ie c a?aooaaiiyi ?acoeueoao?a, iaea?aeaieo i?e aeine?aeaeaii? iaoeaii? nia?ie, oiaoi aeei?enoiaoaaee aaioai?oeei ? aii?oeee?i a eiioeaio?aoe?yo 25 iea ? 100 IAe ia 1 ie na?aaeiaeua, a?aeiia?aeii. A aeine?ae? c? nia?iith, caii?iaeaiith a iaeeoeueiaaieo a?aioeao, aeaeeaai?noue i?e aeiaeaaaii? aioea?ioee?a o?eueee a na?aaeiaeua ?1 aoea aeua ia 0,7 aiae., aaniethoiee iieaciee aeaeeaaiino? – ia 2,4 iae. a ii??aiyii? c eiio?ieai ? ia 0,5 aiae., 2,5 iae. a ii??aiyii? c aeiaeaaaiiyi aioea?ioee?a a iaeaeaa na?aaeiaeua. E??i oeueiai, eiao?oe??io oiene/iino? i?e aeiaeaaaii? aioea?ioee?a o?eueee a ia?oa na?aaeiaeua neeaa 2,1, a i?e aeiaeaaaii? a iaeaeaa na?aaeiaeua - 2,5, oiaoi aeiaeaaaiiy aioea?ioee?a a inoaiiueiio aeiaaeeo aoei oiene/iei aeey nia?i??a (ii?ia 1,8-2,4). I?e aeine?aeaeaii? nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao, aoei anoaiiaeaii, ui aeiaeaaaiiy aioea?ioee?a aii?oeee?io ? aaioai?oeeio a eiioeaio?aoe?? a?aeiia?aeii 25 iea ? 100 IAe ia 1 ie na?aaeiaeua ia?aaeuoaaei eiio?ieuei? iieacieee aeaeeaaiino? ia 0,9 aiae. (?<0,001) ? aaniethoiee iieaciee aeaeeaaiino? – ia 1,8 iae. (?<0,01), ia ia?aaeuoth/e i?e oeueiio ii?i oiene/iino?. Ianooiiei aoaiii iiooeo na?aaeiaeua aeey naiaoe?? aoei aea/aiiy aieeao aioea?ioee?a ia aeaeeaai?noue ?icii?iaeaii? nia?ie. Aoei anoaiiaeaii, ui aeiaeaaaiiy aii?oeee?io ? aaioai?oeeio a eiioeaio?aoe?yo a?aeiia?aeii 25 iea ? 100 IAe ia 1 ie ?icii?iaeoaa/a (2,9%-iiai ?ic/eio iao??th oeeo?aoo) i?aeaeuoaaei aeaeeaai?noue nia?i??a ia 1,4 aiae. ? aaniethoiee iieaciee aeaeeaaiino? – ia 4,0 iae. (?<0,01), a oaeiae ia aoei oiene/iei ii a?aeiioaiith aei nia?i??a. E??i oeueiai, aoei i?iaaaeaii aea/aiiy aeaiiai na?aaeiaeua i?e caa??aaii? eiai a ??aeeiio acio? ? i?e e?iiaoi?e oaiia?aoo?? i?ioyaii 3-o ae?a. ?acoeueoaoe aeine?aeaeaiue i?eaaaeaii a oaae. 5. Oaaeeoey 5 Aeaeeaai?noue nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao c aeei?enoaiiyi ?icii?iaeoaa/a i?e ??cieo oaiia?aoo?ao eiai caa??aaiiy (n=7) Na?aaeiaeuaAeaeeaai?noue nia?i??a, aiae.Aaniethoiee iieaciee aeaeeaaiino?, iae.Eiio?ieue 1 (oeeo?ao iao??th aac aioea?ioee?a) 5,4±0,28 18,4±1,4Eiio?ieue 2 (na?aeii?eaioiaaiee oeeo?ao iao??th c aioea?ioeeaie) 7,2±0,29 * 23,4±1,3 *Aeine?ae 1 (caa??aaiiy i?e e?iiaoi?e oaiia?aoo??) 6,3±0,29 * 21,5±1,3 *Aeine?ae 2 (caa??aaiiy a ??aeeiio acio?) 7,1±0,26 * 23,1±1,3 ** - P<0,01 Aoei anoaiiaeaii, ui oi/a iieacieee aeaeeaaiino? ?icii?iaeoaa/a, yeee i?noeoue aioea?ioeee, ia cieaeothoueny a ii??aiyii? c eiio?ieai, iaeiae niinoa??aa?oueny iaaaoeaiee aieea ia oe? iieacieee e?iiaoii? oaiia?aoo?e . Oiaoi, aeey caiia?aaiiy cieaeaiiy aeoeaiino? aeey 2,9 %-iiai ?ic/eio oeeo?aoo iao??th, ui i?noeoue aioea?ioeee, iioeiaeueiei ? caa??aaiiy eiai a ??aeeiio acio? i?e oaiia?aoo?? - 196°N. I?e ii??aiyii? cai?iiiiiaaiiai iaie eiiieaeno aioea?ioee?a aaioai?oeei+aii?oeee?i c ?ioei i?aia?aoii aeey naiaoe?? nia?ie - nia?iinaiii-3, aoei anoaiiaeaii, ui i?e aeine?aeaeaii? iaoeaii? nia?ie nia?iinai-3 ia?aaeuoaaa eiio?ieue (aac aioea?ioee?a) ca iieacieeaie aeaeeaaiino? ia 1,5 aiae. (?<0,01) ? 2,4 iae. – aaniethoiee iieaciee aeaeeaaiino?. Iaeiae, a ii??aiyii? c eiiieaenii aaioai?oeei+aii?oeee?i aoa iaio aoaeoeaiei ia 0,5 aiae. ? 1,0 iae., a?aeiia?aeii, ia?aaeuoth/e i?e oeueiio ii?io oiene/iino? (2,4 i?ioe 2,1 - aeey eiiieaeno aaioai?oeei+aii?oeee?i). A aeine?ae? c caii?iaeaii-a?aeoaiooith nia?iith niinoa??aaeany oa ae oaiaeaioe?y, iaeiae i?e oeueiio, nia?iinai-3 i?iyaeya a?eueo oiene/io ae?th (2,6 aeey nia?iinaia-3 ? 2,2 - aeey eiiieaeno aaioai?oeei+aii?oeee?i). Oiaoi a?eueo aoaeoeaiei aeyaeany i?iiiiiaaiee iaie eiiieaen aioea?ioee?a aaioai?oeei+aii?oeee?i. Ianooiiei aoaiii iaoeo aeine?aeaeaiue aoei aea/aiiy i?e?iaii? caa?oaeiaiino? nia?ie. A oiae? aeine?aeo aoei anoaiiaeaii, ui iaoeaia nia?ia i?noeoue a na?aaeiueiio 839,5±36,8 i?e?iaieo o?e a 1 ie, ui ia ia?aaeuo? aeiia aea?aenoaiaea?oo 23745-79 aeey iaoeaii? nia?ie. I?ney iai?ioeyoe?e ? caa??aaiiy nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao, nooi?iue i?e?iaii? caa?oaeiaiino? ia?aaeuo? aeiiae aea?aenoaiaea?oo aeey oaeiai aeaeo nia?ie, oiaoi a?eueoa 500 i?e?iaieo o?e a 1 ie. Aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi ia caaaeaee caaacia/o? aeiia a?aeiinii i?e?iaiiai /enea (a na?aaeiueiio 409,5±40,6, a aeayeeo aeiaaeeao a?eueoa 500 i?e?iaieo o?e a 1 ie). Aaeoa??aeueia caa?oaeiai?noue nia?ie, caii?iaeaii? aac aioea?ioee?a ? ?icii?iaeaii? c aeiaeaaaiiyi aii?oeee?io ? aaioai?oeeio, neeaaeaea a na?aaeiueiio 276,3±69,2 i?e?iaieo o?e a ie, ui a?aeiia?aea? aaoa?eia?ii-nai?oa?iei aeiiaai aeey oaeiai aeaeo e??ieiina?aiaaii? nia?ie. E??i oeueiai, iaie aoei i?iaaaeaii aaeoa??ieia?/ia aeine?aeaeaiiy aeiaoeiai-eaeoiciiai na?aaeiaeua, yea canoiniaoaaee aeey ?icaaaeaiiy nia?ie ia?aae caii?iaeoaaiiyi a a?aee?eoeo a?aioeao. Aoei anoaiiaeaii, ui na?aeii?eaioiaaia na?aaeiaeua ? noa?eeueiei, i?e caa??aaii? ae eiai a ??aeeiio acio? o aeaeyae? a?aee?eoeo a?aioe noa?eeuei?noue caaacia/o?oueny eeoa ca oiiae aeiaeaaaiiy aioea?ioee?a. Oiaoi, oe? aeai? na?ae/aoue i?i oa, ui aeiaoeiai-eaeoicia na?aaeiaeua ia ? aeaea?aeii i?e?iaiiai caa?oaeiaiiy nia?ie i?e caii?iaeoaaii? ?? a aeaeyae? a?aee?eoeo a?aioe. A??ia?aeiei aeaea?aeii caa?oaeiaiiy ? ??aeeee acio, oae ye i?e caa??aaii? a iueiio na?aaeiaeua, yea ia i?noeei aioea?ioee?a i?e?iaia caa?oaeiai?noue i?aeaeuoaaeany aei 934,6±27 i?e?iaieo o?e a ie. Eie? oeo? i?e aeine?aeaeaii? an?o i?ia ia ia?aaeuoaaa 0,1, ui a?aeiia?aea? aeiiaai aea?aenoaiaea?oo 23745-79 aeey iaoeaii? ? 26030-83 - aeey caii?iaeaii-a?aeoaiooi? nia?ie. Ianooiiei aoaiii iiooeo naioth/iai caniao aoei aecia/aiiy caie?aeithaaeueii? caeaoiino? nia?ie, yea i?noeea aaioai?oeei ? aii?oeee?i. ?acoeueoaoe aeine?aeo i?eaaaeaii a oaae. 6 oa 7. Oaaeeoey 6 Caie?aeithaaeueia caeaoi?noue nia?ie i?e aeiaeaaaii? aioea?ioee?a ia?aae caii?iaeoaaiiyi a iaeeoeueiaaieo a?aioeao Nia?iaEi?iaeOaeeoe?Ei?iae ? oaeeoe?in?ia-iaiio?eueieo%in?ia-iaiio?eueieo%in?ia-iaiio?eueieo%Ae/a “Eoooc?aea”1.Aeiaeaaaiiy aioea?ioee?a a na?aaeiaeua ?1 24 12 50±10,2 27 20 74±8,4 51 32 62±6,92.Aeiaeaaaiiy aioea?ioee?a a iaeaeaa na?aaeiaeua 21 7 33±10,3 31 19 61±8,8 52 26 50±7,03.Eiio?ieue22627±9,5311961±8,8532547±6,9A?ae. “O?oica”1.Aeiaeaaaiiy aioea?ioee?a a na?aaeiaeua ?1 17 7 41±11,9 18 13 72±10,6 35 20 57±8,52.Aeiaeaaaiiy aioea?ioee?a a iaeaeaa na?aaeiaeua 16 10 62±12,1 21 13 62±10,6 37 23 62±8,13.Eiio?ieue16953±12,515960±12,6311858±9,0A na?aaeiueiio ii aeaio ainiiaea?noaao1.Aeiaeaaaiiy aioea?ioee?a a na?aaeiaeua ?1 41 19 46±7,8 45 33 73±6,6 86 52 60±5,32.Aeiaeaaaiiy aioea?ioee?a a iaeaeaa na?aaeiaeua 37 17 46±8,2 52 32 62±6,7 89 49 55±5,33.Eiio?ieue381539±7,9462861±7,2844351±5,5 C iaea?aeaieo aeaieo aeaeii, ui aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi a iaeeoeueiaaieo a?aioeao ? aoaeoeaiei, ye i?e aeiaeaaaii? a ia?oa, oae ? a iaeaeaa na?aaeiaeua. Iaeiae, a?eueo aoaeoeaiei ? aeiaeaaaiiy a na?aaeiaeua ?1 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io, oae ye i?e aeiaeaaaii? aioea?ioee?a a iaeaeaa na?aaeiaeua, ii-ia?oa, caie?aeiai?noue aoea ieae/a, i?ae i?e aeiaeaaaii? aioea?ioee?a o?eueee a na?aaeiaeua ?1 ?, ii-ae?oaa, ye aoei anoaiiaeaii ?ai?oa, aeiaeaaaiiy aioea?ioee?a a iaeaeaa na?aaeiaeua ? oiene/iei ii a?aeiioaiith aei nia?i??a. I?e aeine?aeaeaii? nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao anoaiiaeaii (aeea. oaae. 7), ui iioeiaeueiei ? aeiaeaaaiiy aioea?ioee?a o?eueee i?e ?icii?iaeoaaii?, oae ye i?e aeiaeaaaii? aioea?ioee?a ia?aae caii?iaeoaaiiyi caie?aeiai?noue aoea ieae/a, a aeiaeaaaiiy aioea?ioee?a iaeii/anii i?e caii?iaeoaaii? ? ?icii?iaeoaaii?, ye anoaiiaeee ?ai?oa, aoei oiene/iei ii a?aeiioaiith aei nia?i??a. Oaaeeoey 7 Aeiaeaaaiiy aioea?ioee?a i?e caii?iaeoaaii? ? ?icii?iaeoaaii? nia?ie a a?aee?eoeo a?aioeao Nia?iaIn?ia iaiiO?eue- Ieo%Ae/a “Eoooc?aea” (1-e aeine?ae)1. Aeiaeaaaiiy aioea?ioee?a i?e ?icii?iaeoaaii?382155±8,12. Aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi462350±7,43. Eiio?ieue (aac aeiaeaaaiiy aioea?ioee?a)371540±8,1A?ae. “O?oica”1. Aeiaeaaaiiy aioea?ioee?a i?e ?icii?iaeoaaii?13754±13,82. Aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi14643±13,23. Eiio?ieue (aac aeiaeaaaiiy aioea?ioee?a)13538±13,5Ae/a “Eoooc?aea” (2-e aeine?ae)1. Aeiaeaaaiiy aioea?ioee?a i?e ?icii?iaeoaaii?221045±10,62. Aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi10440±15,53. Eiio?ieue (aac aeiaeaaaiiy aioea?ioee?a)8337±17,1A na?aaeiueiio ii aeaio ainiiaea?noaao1. Aeiaeaaaiiy aioea?ioee?a i?e ?icii?iaeoaaii?733852±5,92. Aeiaeaaaiiy aioea?ioee?a ia?aae caii?iaeoaaiiyi703347±6,03. Eiio?ieue (aac aeiaeaaaiiy aioea?ioee?a)582340±6,4 A?aoiaoth/e ?ai?oa iaea?aeai? aeai? ii aeaeeaaiino?, i?e?iai?e caa?oaeiaiino?, caie?aeiaiino? nia?ie i?e aeiaeaaaii? aioea?ioee?a, a oaeiae ?o oiene/iino? ii a?aeiioaiith aei nia?i??a ? /ooeeaino? aei ieo i?e?ioei?e, iaie aoei ?ic?iaeaii naioth/a na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie aoaa?a, caii?iaeaii? a a?aee?eoeo a?aioeao (i??i?eoaoia aeia?aeea ia iaoaio ?98062850 a?ae 02.06.1998 ?.) ? i?iaaaeaii ae?iaie/ee aeine?ae ii eiai canoinoaaiith. ?acoeueoaoe aeine?aeo i?eaaaeaii a oaae. 8. Aeaiei na?aaeiaeuai ? 2,9%-aee ?ic/ei iao??e eeiiiiieeneiai o?ecai?uaiiai i’yoeaiaeiiai, a yeee c iaoith iaaeaiiy eiio aioei?e?iaieo aeanoeainoae ie aaaee aioea?ioeee oe?ieiai niaeo?o ae?? aaioai?oeei oa aii?oeee?i a eiioeaio?aoe?? 25 iea/ie ? 100 IAe/ie, a?aeiia?aeii. E??i oeueiai, c iaoith a?eueo o?eaaeiai caa??aaiiy iei aioei?e?iaieo aeanoeainoae eiai caa??aathoue a ??aeeiio acio? i?e oaiia?aoo?? - 196°N. Oaaeeoey 8 Caie?aeiai?noue ei??a i?e aeei?enoaii? naioth/iai na?aaeiaeua A?oie oaa?eiIn?iaiaiiO?eueieo%A?ae. “O?oica”1. Aeine?ae944447±5,22. Eiio?ieue 742736±5,6AeIC “*a?aiiee Aaeaoaiue”1.Aeine?ae **16511872±3,52. Eiio?ieue1508154±4,1A na?aaeiueiio ii aeaio ainiiaea?noaao1. Aeine?ae *25916262±3,02. Eiio?ieue 22410846±3,3* - P<0,01;** - P<0,001 I?e aeei?enoaii? aeiaaicaa?aaeaiiai na?aaeiaeua aoei in?iaiaii a caaaeuei?e e?eueeino? 259 aie?a, c yeeo o?eueieie aeyaeeiny 162 aieiae (62%). A eiio?ie? aoei in?iaiaii 224 aieiae, c yeeo o?eueieie aeyaeeiny 108 aie?a (48%). Oiaoi, canoinoaaiiy naioth/iai na?aaeiaeua i?aeaeuoaaei caie?aeiai?noue nia?ie ia 14%. Aeniiaee 1. An? aeine?aeaeai? na?aaeiaeua (TALP, BWW, A??inoa?a, ONI-199 ? E?aan-??iaa?a) aeey eoeueoeaoaaiiy iioeeo?a i?aeaeuoaaee aeaeeaai?noue iaoeaii? ? caii?iaeaii-a?aeoaiooi? nia?ie. Iaea?eueo aoaeoeaiei aoei na?aaeiaeua TALP, i?e aeei?enoaii? yeiai aeaeeaai?noue aoea aeua ia o?eueee a ii??aiyii? c eiio?ieai, aea e a ii??aiyii? c ?ioeie aeine?aeieie na?aaeiaeuaie. 2. An? aeine?aeaeai? aeiaaaee (aaia?ei, eioa?i, ai?oae?e iaoee ? yeoeaaiae?a, oaoaeueia ne?iaaoea ? oie?eoey?ia ??aeeia), ye? canoiniaothoueny aeey eoeueoeaoaaiiy in vitro, i?aeaeuoaaee aeaeeaai?noue nia?i??a. Iaea?eueo aoaeoeaiith c canoiniaaieo aeiaaaie aoei aeiaeaaaiiy ee?oei ai?oae?th yeoeaaiaeo ei??a a eiioeaio?aoe?? 25 %, eiee iieacieee aeaeeaaiino? aoee aeua ye eiio?ieueieo, oae ? ?ioeo aeine?aeieo. 3. A i?ioean? eaiaoeeoaoe?? (ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro) aeaeeaai?noue nia?i??a iaoeaii? ? caii?iaeaii-a?aeoaiooi? nia?ie aoaa?a ia cieaeoaaany a ii??aiyii? c eiio?ieai, a iaa?oue iaea oaiaeaioe?th aei iie?auaiiy. 4. I?ioean ia?iaee nia?i??a ia?aae caie?aeiaiiyi in vitro ia /eiea iaaaoeaiiai aieeao ia ?acoeueoaoeai?noue e??ieiina?aaoe??, oiaoi ia aeaeeaai?noue ? caie?aeiai?noue nia?ie. 5. Aeey i?eai?/aiiy i?e?iaii? eiioai?iaoe?? iaoeaii? nia?ie a?aeiia?aeii aei ii?i aea?aenoaiaea?oo iioeiaeueiei aoei aeiaeaaaiiy aioea?ioee?a aaioai?oeeio ? aii?oeee?io a eiioeaio?aoe?? a?aeiia?aeii 25 iea oa 100 IAe ia 1 ie na?aaeiaeua. 6. I?e caii?iaeoaaii? nia?ie a iaeeoeueiaaieo a?aioeao ?? naiaoe?? iioeiaeueiei aoei aeiaeaaaiiy 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie na?aaeiaeua ?1 ia?aae caii?iaeoaaiiyi, ui i?aeaeuoaaei ?? aeaeeaai?noue i?ney aeaeiina?aaoe?? a ii??aiyii? c? nia?iith, yea ia i?noeea aioea?ioee?a ? c aeiaeaaaiiyi aioea?ioee?a a iaeaeaa na?aaeiaeua. 7. Aeey naiaoe?? nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao, iioeiaeueiei aoei aeiaeaaaiiy aioea?ioee?a i?e ?icii?iaeoaaii? a eiioeaio?aoe?? 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie 2,9 %-iiai ?ic/eio iao??th eeiiiiieeneiai o?ecai?uaiiai i’yoeaiaeiiai. 8. ??aaiue i?e?iaii? caa?oaeiaiino? a a?aeiia?aeiino? aei aeiia aea?aenoaiaea?oo aeey nia?ie, caii?iaeaii? a a?aee?eoeo a?aioeao, caaacia/oaaany eeoa i?e aeiaeaaaii? aioea?ioee?a a i?ioean? ?icii?iaeoaaiiy. 9. ?ic?iaeaii aoaeoeaia aioei?e?iaia na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie aoaa?a, yea caaacia/o? ??aaiue i?e?iaii? caa?oaeiaiino? a?aeiia?aeii aei aeiia aea?aenoaiaea?oo, a oaeiae i?aeaeuo? caie?aeiai?noue ia 11-18 %, caa??aath/e i?e oeueiio aaeoa?eoeeaeio ae?th ia i?ioyc? o?eaaeiai /ano caaaeyee caa??aaiith ?? i?e oaiia?aoo?? - 196°N. I?iiiceoe?? ae?iaieoeoao 1. Aeey naiaoe?? nia?ie aoaa?a c iaoith i?aeaeuaiiy ?? a?ieia?/ii? oa nai?oa?ii? yeino?, a oaeiae caie?aeithaaiino? ei??a oa oaeeoeue i?iiiio?oueny aeei?enoiaoaaoe aeiaeaaaiiy 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie na?aaeiaeua ?1 i?e caii?iaeoaaii? a iaeeoeueiaaieo a?aioeao. 2. Aeey naiaoe?? nia?ie, caii?iaeaii? o a?aee?eoeo a?aioeao, ?aeiiaiaeo?oueny ?icii?iaeaiiy ?? a 2,9 %-iiio ?ic/ei? iao??th eeiiiiieeneiai o?ecai?uaiiai i’yoeaiaeiiai c aeiaeaaaiiyi 25 iea aaioai?oeeio ? 100 IAe aii?oeee?io ia 1 ie. 3. C iaoith iiia?aaeaeaiiy cieaeaiiy aaeoa?eoeeaeiino? 2,9 %-iiai ?ic/eio iao??e eeiiiiieeneiai o?ecai?uaiiai i’yoeaiaeiiai, yeee i?noeoue aioea?ioeee, i?iiiio?oueny caa??aaoe eiai a ??aeeiio acio?. 4. Iaea?aeai? aeai? iiaeia aeei?enoiaoaaoe a AOCao aeey /eoaiiy eaeoe?e. Nienie iioae?eiaaieo ?ia?o ia oaio aeena?oaoe?? 1. Neey?ia I.I. Aieea eiiieaeno aioea?ioee?a aaioai?oeei+aii?oeee?i ia ia?aaeeaa?i?noue oa caie?aeithaaeueio caeaoi?noue nia?i??a aoaa?a-ie?aeiee?a //Ca. iaoeiaeo i?aoeue “I?iaeaie cii?iaeaia??? oa aaoa?eia?ii? iaaeeoeeie”. - Oa?e?a. - 1998. - Aei. 3. - N.54-58. 2. Neey?ia I.I. Ii?aaeaeaiea aeeyiey aioeaeioeeia (aiieoeeeeeia e aaioaieoeeia) ia ia?aaeeaaaiinoue nia?ieaa aueia //Iaoeiai-oaoi?/iee atheaoaiue ?O OAAI. - Oa?e?a. - 1998. - ?74. - N.44-48. 3. Aoa?ia I.Ae., Neey?ia I.I. Aeiaeaaaiiy aioea?ioee?a a nia?io aoaa?a //Ca. iaoeiaeo i?aoeue “I?iaeaie cii?iaeaia??? oa aaoa?eia?ii? iaaeeoeeie”. - Oa?e?a. - 1998. - Aeione 3. - N.52-54. 4. Aoa?ia A.Ae., Neey?ia I.I. E?eieiina?aaoeey eaiaoeeoe?iaaiiuo nia?ieaa //Ca. iaoa??ae?a ?? I?aeia?iaeii? eiioa?aioe?? “Aeei?enoaiiy no/anieo iieaeoey?ii-aaiaoe/ieo ? a?ioaoiieia?/ieo ?ic?iaie o aaiaoeei-naeaeoe?eieo aeine?aeaeaiiyo”. – Iaeana. – 1998. – N.85-86. 5. Aoa?ia A.Ae., Neey?ia I.I. I?aaeoi?aaeaeaiea iee?iaiie caa?yci?iiinoe nia?iu aueia //Ca. iaoa??ae?a ?? I?aeia?iaeii? eiioa?aioe?? “Aeei?enoaiiy no/anieo iieaeoey?ii-aaiaoe/ieo ? a?ioaoiieia?/ieo ?ic?iaie o aaiaoeei-naeaeoe?eieo aeine?aeaeaiiyo”. – Iaeana. – 1998. – N.88-90. 6. Aoa?ia A.Ae., Neey?ia I.I. ?acoeueoaou inaiaiaiey nia?iie, niaea?aeauae aioeaeioeee //Iaoa??aee I?aeia?iaeii? iaoeiai-i?aeoe/ii? eiioa?aioe??, i?enay/aii? 100-??//th a?ae aeiy ia?iaeaeaiiy I.TH. ssoeaiea. – Oa?e?a. – 1998. – N.119-120. 7. Aoa?ia I.Ae., Oeoiia A.N., Neey?ia I.I. Aeei?enoaiiy eiiieaeno aioea?ioee?a aaioai?oeeia c aii?oeee?iii aeey aeaeiioai?iaoe?? aia??ii?a ei??a oa nia?ie aoaa?a //Ca. noaoae I?aeia?iaeii? iaoeiai-i?aeoe/ii? eiioa?aioe??, i?enay/aii? 100-??//th Euea?anueei? aaoa?eia?ii? aeaaeai?? ?i. N.C. Aaeeoeueeiai “No/ani? i?iaeaie a?ieia??, aaoa?eia?ii? iaaeeoeeie, cii?iaeaia??? oa oaoiieia?? i?iaeoeo?a oaa?eiieoeoaa”. – Euea?a. – 1997. – N.120-121. 8. Aoa?ia A.Ae., Oeoiia A.N., Neey?ia I.I. Eniieueciaaiea eiiieaena aioeaeioeeia aaioaieoeeia e aiieoeeeeeia aeey naiaoeee nia?iu e yia?eiiia //Iaoa??aee I?aeia?iaeii? iaoeiai-ae?iaie/i? eiioa?aioe?? “Aeei?enoaiiy o?ainieaioaoe?? aia??ii?a a naeaeoe?? ? a?aeoai?aii? n.- a. oaa?ei”. – Ee?a, Aneai?y-Iiaa. – 1997. – N.78-79. 9. Cayaea ia aeiao?ae ?98062850 a?ae 02.06.1998 ?. c i??i?eoaoii //Aoa?ia I.Ae., Neey?ia I.I. /Naioth/a na?aaeiaeua aeey ?icii?iaeoaaiiy nia?ie n?euenueeiainiiaea?nueeeo oaa?ei. Neey?ia I.I. Aieea aioea?ioee?a ? na?aaeiaeu aeey eoeueoeaoaaiiy iioeeo?a in vitro ia ye?ni? iieacieee nia?ie aoaa?a. Aeena?oaoe?y (?oeiien) ia caeiaoooy iaoeiaiai nooiaith eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe ca niaoe?aeuei?noth 03.00.20. – a?ioaoiieia?y. ?inoeooo oaa?eiieoeoaa OAAI. Oa?e?a, 1998. A aeena?oaoe?? i?eaaaeaii aeai? i?i ii??aiyeueia aea/aiiy aeaeeaaiino? nia?ie i?e aeei?enoaiiy na?aaeiaeu oa aeiaaaie aeey eoeueoeaoaaiiy iioeeo?a in vitro. ?icaeyiooi ieoaiiy noiniaii aeaeeaaiino? nia?i??a a i?ioean? ia?iaee ?o ia?aae caie?aeiaiiyi in vitro (eaiaoeeoaoe??) ? aieeao oeueiai i?ioeano ia ?acoeueoaoeai?noue e??ieiina?aaoe??. ?ic?iaeaii aoaeoeaiee i?aia?ao aeey naiaoe?? nia?ie aoaa?a c? ai?noii 25 iea/ie aaioai?oeeia ? 100 IAe/ie aii?oeee?i , yeee caaacia/o? i?aeaeuaiiy caie?aeiaiino? nia?ie ia 11-18%. C iaoith caa??aaiiy aioei?e?iaieo aeanoeainoae cai?iiiiiaaii eiina?aoaaoe eiai a ??aeeiio acio? i?e oaiia?aoo?? - 196°N. Eeth/ia? neiaa: nia?ia, nia?i??, na?aaeiaeua, aeiaaaea, aeaeeaai?noue, eaiaoeeoaoe?y, e??ieiina?aaoe?y, caie?aeiai?noue, naiaoe?y. Neey?ia I.I. Aeeyiea aioeaeioeeia e n?aae aeey eoeueoeae?iaaiey iioeeoia in vitro ia ea/anoaaiiua iieacaoaee nia?iu aueia. Aeenna?oaoeey (?oeiienue) ia nieneaiea o/?iie noaiaie eaiaeeaeaoa naeueneioicyenoaaiiuo iaoe ii niaoeeaeueiinoe 03.00.20. – aeioaoiieiaey. Einoeooo aeeaioiiaiaenoaa OAAI. Oa?ueeia, 1998. A aeenna?oaoeee i?eaaaeaiu aeaiiua i n?aaieoaeueiii eco/aiee auaeeaaaiinoe nia?iu i?e eniieueciaaiee n?aae e aeiaaaie aeey eoeueoeae?iaaiey iioeeoia in vitro. Onoaiiaeaii iieiaeeoaeueiia aeeyiea n?aae (TALP, BWW, A?einoa?a, ONI-199, E?aan-?eiaa?a) e aeiaaaie (yieoaeey iaoee e yeoeaaiaeia, oieeeeoey?iie aeeaeeinoe, oaoaeueiie nuai?ioee, aaia?eia, eioaeia), eioi?ua eniieuecothony aeey eoeueoeae?iaaiey iioeeoia e yia?eiiia in vitro ia auaeeaaaiinoue iaoeaiie e e?eieiina?ae?iaaiiie nia?iu aueia. Iaeaieaa yooaeoeaiie yaeyaony n?aaea TALP, a ec aeiaaaie - yieoaeee yeoeaaiaea a eiioeaio?aoeee 25%. ?anniio?ai aii?in, eanathueeny auaeeaaaiinoe nia?ieaa a i?ioeanna ia?aaioee eo ia?aae iieiaeioai?aieai in vitro (eaiaoeeoaoeee) e aeeyiea yoiai i?ioeanna ia ?acoeueoaoeaiinoue e?eieiina?aaoeee. Aeieacaii, /oi i?ioeann ia?aaioee ia?aae iieiaeioai?aieai in vitro ia ieacuaaao io?eoeaoaeueiiai aeeyiey ia auaeeaaaiinoue nia?ieaa ia?aae e iinea e?eieiina?aaoeee, a oaeaea ia eo iieiaeioai?ythuoth niiniaiinoue. Onoaiiaeaii iieiaeeoaeueiia aeeyiea eiiieaena aioeaeioeeia aaioaieoeeia e aiieoeeeeeia ia auaeeaaaiinoue naaaea?acaaaeaiiie e caii?iaeaiii-iooayiiie nia?iu aueia. Ii?aaeaeaii iioeiaeueioth eiioeaio?aoeeth aaioaieoeeia (25 iea/ie) e aiieoeeeeeia (100 AAe/ie), eioi?ua nieaeatho iee?iaioth eiioaieiaoeeth nia?iu aueia n nio?aiaieai a? iieiaeioai?ythuae niiniaiinoe. ?ac?aaioai yooaeoeaiue i?aia?ao aeey naiaoeee nia?iu aueia, eioi?ue iaania/eaaao iiauoaiea iieiaeioai?yaiinoe nia?iu ia 11-18%. N oeaeueth nio?aiaiey aioeiee?iaiuo naienoa i?aaeeiaeaii eiina?aaoeey aai a aeeaeeii acioa i?e oaiia?aoo?a - 196°N. Eeth/aaua neiaa: nia?ia, nia?iee, n?aaea, aeiaaaea, auaeeaaaiinoue, eaiaoeeoaoeey, e?eieiina?aaoeey, iieiaeioai?yaiinoue, naiaoeey. Sklyarov P.M. Effect of antibiotics and media for in vitro maturation of oocytes on qualitative characteristics of bull sperm. The dissertation (manuscript) for the degree of “Candidate of Agricultural Sciences” in speciality 03.00.20. – biotechnology. Institute of Animal Science. Kharkov, 1998. Data of a comparative study on sperm survival when medium and additions in vitro oocytes are used are given in the dissertation. The problem of spermatozoa survival in the course of their treatment before in vitro fertilization and influence of this process on cryopreservation was considered. The effective preparation for bull semen sanation with 25 mg/mlgentamycin and 100 UE/ml ampicillin, which provided increase in bull semen fertility by 11-18% was developed. It was proposed to preserve in liquid nitrogen at temperature - 196°C to maintain its antimicrobial properties. Key words: sperm, spermatozoa, medium, additive, survival, capacitation, cryopreservation, fertilizing ability, sanation. A?aeiia?aeaeueiee ca aeione: aeieoi? a?ieia?/ieo iaoe, i?ioani?, /eai-ei?aniiiaeaio OAAI I.?.Naoaoeueeee I?aeienaii aei ae?oeo 25.11.98?.Oi?iao 60o90/16,Oi.ae?oe.a?e. 1.0. Ae?oe ionaoiee. Aa?i?oo?a Oaein. Oe?aae 100 i?ei. Cai. ?112. ?AA OCA?, Oa?e?anueea iae., Aea?aa/?anueeee ?-i, i/a Iaea Aeaiee?aea, Oa?e?anueeee ciiaaoa?eia?iee ?inoeooo

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