Структурно-функціональні дослідження ліпополісахаридів Ralstonia solanacearum: Автореф. дис… канд. біол. наук / Н.В. Москаленко, НАН України. Ін-т м

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OAeE 579.841.11.22

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E(IIIIE(NAOA?EAe(A Ralstonia solanacearum

03.00.07 — i(e?ia(ieia(y

AAOI?AOA?AO

aeena?oaoe(( ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa a(ieia(/ieo iaoe

EE(A — 1999

Aeena?oaoe((th ( ?oeiien.

?iaioa aeeiiaia a (inoeooo( i(e?ia(ieia(( ( a(?onieia(( (i.
Ae.E.Caaieioiiai IAI Oe?a?ie, i. Ee(a.

Iaoeiaee ea?(aiee :

aeieoi? a(ieia(/ieo iaoe,

AA?AAIAOeUe Ethaeieea Aeieo?(aia

(inoeooo( i(e?ia(ieia(( ( a(?onieia(( (i. Ae.E.Caaieioiiai,

caa(aeoaa/ a(aeae(eii a(io(i(( i(e?ii?aai(ci(a

Io(oe(ei( iiiiaioe :

/eai-ei?aniiiaeaio IAI Oe?a(ie, aeieoi? a(ieia(/ieo iaoe, i?ioani?
IAEAOAIEI TH?(e ?iiaiiae/

(inoeooo i(e?ia(ieia(( ( a(?onieia(( (i. Ae.E.Caaieioiiai,

caa(aeoaa/ a(aeae(eii a(ieia(( aaciieenethth/eo i(e?ii?aai(ci(a

aeieoi? iaaee/ieo iaoe, i?ioani?

AI?ENIA Aaaeei Aiaoieueiae/

Ee(anueeee oi(aa?neoao (i. O.A.Oaa/aiea,

i?ioani? eaoaae?e i(e?ia(ieia(( oa caaaeueii( (ioiieia((

I?ia(aeia onoaiiaa

Iaeanueeee aea?aeaaiee oi(aa?neoao (i. (.(.Ia/ieeiaa, eaoaae?a
i(e?ia(ieia((.

Caoeno aeena?oaoe(( a(aeaoaeaoueny “ 20_” aeiaoiy__ 1999 ?. i 10.00
aiaeei( ia can(aeaii( niaoe?ae(ciaaii( a/aii( ?aaee Ae 26.233.01 a
(inoeooo( i(e?ia(ieia(( ( a(?onieia(( (i. Ae.E.Caaieioiiai IAI Oe?a(ie
ca aae?anith: 252143, i.Ee(a, aoe. Caaieioiiai, 154.

C aeena?oaoe((th iiaeia iciaeiieoenue a a(ae(ioaoe( (inoeoooo
i(e?ia(ieia(( ( a(?onieia(( (i. Ae.E.Caaieioiiai IAI Oe?a(ie ca
aae?anith: 252143, i.Ee?a, aoe. Caaieioiiai, 154.

Aaoi?aoa?ao ?ic(neaiee _17 aa?aniy_ 1999 ?.

A/aiee nae?aoa?

niaoe(ae(ciaaii( a/aii( ?aaee

eaiaeeaeeo a(ieia(/ieo iaoe
E.I.Io?(o

Aeooaeuei?noue i?iaeaie. E?iiiie?naoa?eaee (EIN) yaeythoueny
niaoeeo(/ieie eiiiiiaioaie ee?oeiii? iaieiiee a?aiiaaaoeaieo aaeoa??e.
Aiie ?icoaoiaai( ia ciai?oi?e iiaa?oi( iaia?aie i?e?ii?aai?cio ?
aiane?aeie nai(( oi?eaeueii? aoaeiae aeeiiothoue ?yae aaaeeeaeo aeey
ee?oeie o?ceei-o?i?/ieo ? a?ieia?/ieo ooieoe?e. EIN a?ae?a?athoue cia/io
?ieue o i?aeo?eioe? oe?e(niino? iaia?aie, ?aaoeththoue ?? i?iieei?noue
aeey ??cieo niieoe, i?eeiathoue aaaeeeao o/anoue o eiioaeoao
i?e?ii?aai?cio c ?ioeie iae?i- oa i(e?ii?aai(ciaie. Caaaeyee
iiaa?oiaaiio ?icoaooaaiith ? iniaeeainoyi aoaeiae aecia/athoue
I-aioeaaiio niaoeeo?/i?noue aaeoa??e.

Iaeiei (c iiyniaiue i?iyao oaeiai oe?ieiai niaeo?o a(ieia(/ii(
aeoeaiino( ( ?(ciiiai(oi(noue iieaeoey?ieo oi?i EIN a iaaeao iaeiiai
aeaeo, ui iaoiiaeaii aaoa?iaaii(noth (o no?oeoo?ieo /anoei, a naia
I-niaoeeo(/iiai iie(naoa?eaeo (I-IN), ei?iaiai ie(ainaoa?eaeo (ei?-IA)
oa e(i(aeiiai eiiiiiaioo. Aea/aiiy no?oeoo?ii( i?aai(caoe(( ?(cieo
iieaeoey?ieo oi?i EIN iaei((( eoeueoo?e oa c’ynoaaiiy (o o(i(/ii(
i?e?iaee ni?eythoue ?icoeo?oaaiith aeaoa?i(iaio a(ieia(/ii( aeoeaiino( (
iaoai(ci(a ooieoe(iioaaiiy EIN i?e ?aae(caoe(( a(ieia(/ieo i?ioean(a, ui
i?ioiaeyoue ca (o o/anoth.

Neeaae ? no?oeoo?a EIN ? ia nueiaiaei? iaeiei ?c aeciaieo
oaiioaeniiii?/ieo e?eoa???a, ui aeei?enoiao?oueny a nenoaiaoeoe?
a?aiiaaaoeaieo aaeoa??e. O(i(/ia ?(ciiiai(oi(noue oei(a no?oeoo?
I-niaoeeo(/iiai iie(naoa?eaeo EIN iaoiiaeth( na?ieia(/io
niaoeeo(/i(noue, ui oe?iei aeei?enoiao?oueny i?e noai?aii? aaeoeei ?
ae?aaiinoeeoi?a, a oaeiae i?e ?ic?iaoe? aioo??oiueiaeaeiaeo na?ieia(/ieo
eeaneo?eaoe?eieo noai a?aiiaaaoeaieo i?e?ii?aai?ci?a, a oiio /ene?
o?oiiaoiaaiieo.

Ana aeuacaaaeaia oe(eeii noino(oueny oaeiai aaoa?iaaiiiai ca
aeanoeainoyie ( ua iaaeinoaoiuei aea/aiiiai o(oiiaoiaaiiiai aeaeo, ye
Ralstonia solanacearum.Oeae i?e?ii?aai?ci aeeeeea? aaeoa??aeueia
c?a’yeaiiy oe?ieiai niaeo?o ?ineei ( aei 200 aeae?a). Iniaeeai
iaaacia/iee a?i aeey i?aaenoaaiee?a ?iaeeie ianeueiiiaeo. Oai?iaa, yeo
a(i ni?e/eiy( iaiineoue cia/i( caeoee n(euenueeiio ainiiaea?noao.
Iae/ano(oa aiia ia( iacai?ioi(e oa?aeoa? ( a(eueo(noue (io(eiaaieo
?ineeie aeia.

Iniaeea?noth aeaeo R. solanacearum ? ooaiiaa ??ciiiai?ooy a?ieia?/ieo
aeanoeainoae. Oea ?iaeoue iiaeeeaei ?iciiae?eaiiy ooai(a ia ?ane,
a?iaa?e, iaoiaa?e, na?iaa?e ca oeiii eieii?e, niaoe?ae?caoe?? aei oaa?a
? o. ?. Aea/aiiy o?ceei-o?i?/ieo aeanoeainoae oeueiai o?oiiaoiaaio,
cie?aia iniaeeainoae aoaeiae ( a(ieia(/ii( aeoeaiino( EIN ooai(a R.
solanacearum ? aeooaeueiith i?iaeaiith. Iaea?aeai? aiane?aeie oaeeo
aeine?aeaeaiue a?aeiiino? iiaeooue aooe aeei?enoai? aeey ?ic?iaee
iaoiae?a ae?aaiinoeee ooai?a oeueiai aeaeo, ae?(oaiiy ieoaiue eiai
oaeniiii((, a oaeiae i?e aeine(aeaeaiiyo, ni?yiiaaieo ia aea/aiiy
iaoai(ci(a iaoiaaiaco oai?iae, yeo aeeeeea( oeae i(e?ii?aai(ci.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie ?iaioa
aeeiioaaeanue o a?aeae?e? a?io?i?? i?e?ii?aai?ci?a (inoeoooo
i?e?ia?ieia?? ( a??onieia?? (i. Ae.E.Caaieioiiai ( aeeth/a(
aeine(aeaeaiiy aeeiiai( ca(aeii ieaio iaoeiai-aeine(aeieo ?ia(o
(inoeoooo ca oaiaie:

“Eco/eoue no?oeoo?iua e aiaeeaoi/iua aeeeiiieeia?u e oa?iaiou iee?iaiie
eeaoee n oeaeueth nicaeaiey iiauo iiaeoiaeia oaiinenoaiaoeee e
?ac?aaioee iiauo aeioaoiieiaee” ( 1988-1992 aa., ? ain. ?aa.
0.188.0.052916);

“Aea/aiiy iieaeoey?ieo iaoai(ci(a ooieoe(iioaaiiy ae(eiiie(ia?(a (
ea?aia(ae?ac, ?ic?iaea a(ioaoiieia(e i?aia?ao(a aeey i?aeoe/iiai
canoinoaaiiy” (1993-1997 ??, 07/11),

a oaeiae eiieo?nieo i?iaeo?a iaoeiai-oaoi?/ieo i?ia?ai:

“?ic?iaeoe iia? aoaeoeai? i?aia?aoe iie?naoa?eae?a aeey i?io?eaeoeee oa
e?eoaaiiy iieieia?/ieo, oieneei-?ioaeoe?eieo caoai?thaaiue,
?ioiiaeao?oeeoieo noai?a” (1992-1994 ??., i?ia?aia “Caei?ia’y ethaeeie”,
? i?iaeoo 1.02.02/015-92) ;

“Aea/eoe ?ioiiiiaeoethth/? aeanoeaino? ae?eiiie?ia??a aaeoa??e,
anoaiiaeoe no?oeoo?o ? eiioi?iaoe?th aeaoa?i?iaio a?ieia?/ii? aeoeaiino?
oa eiino?othaaiiy ia ?o iniia? i?aia?ao?a aeey e?eoaaiiy
?ioiiaeao?oeeoieo noai?a” (1992-1995 ??., Aea?aeaaia iaoeiai-oaoi?/ia
i?ia?aia “A?ioaoiieia?y”, ? i?iaeoo 1.11.05/003-92).

Iaoa ?iaioe. Iaoith aeine(aeaeaiiy aoei aea/aiiy neeaaeo ( no?oeoo?e, a
oaeiae aeayeeo aniaeo(a a(ieia(/ii( aeoeaiino( ((ioiio(i(/i( aeanoeaino?
oa (ioiiiiaeoethth/a ae(y) EIN oa (o ie?aieo no?oeoo?ieo eiiiiiaio(a
ooai(a R. solanacearum — i?aaenoaaiee(a ?(cieo a(iaa?(a, ye(
a(ae?(ciyeenue i(ae niaith oaeiae ca aaia?ao(/iei iioiaeaeaiiyi (
?ineeiith-oacy?iii .

Caaaeaiiy aeine?aeaeaiiy

Iaea?aeaoe EIN oa ?o ie?ai( no?oeoo?i? eiiiiiaioe (c 17 ooai(a R.
solanacearum .

Aea/eoe o(i(/iee neeaae (iiiinaoa?eaeiee, ai?iieeneioiee oa
aee?iieeneioiee) no?oeoo?ieo /anoei iieaeoee EIN ??cieo ooai?a.

Anoaiiaeoe o?i?/io no?oeoo?o I-niaoeeo?/ieo iie?naoa?eae?a
aeine?aeaeoaaieo EIN .

Aea/eoe ?ioiio?i?/i( aeanoeaino( EIN R.. solanacearum c iaoith
anoaiiaeaiiy iiaeeeai( ei?aeyoe(( i(ae na?ieia(/iith aeoeai(noth EIN oa
no?oeoo?ith eiai I-IN.

Aeine(aeeoe (ioiiiiaeoethth/o ((ioa?oa?iiiaaiio, i?ioeiooeeiio) ae(th
EIN oa anoaiiaeoe aianie ie?aieo no?oeoo?ieo eiiiiiaio(a iieaeoee ia
i?iya oe((( ae((.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a.

Aia?oa i?iaaaeaii oe?iea nenoaiaoe/ia aea/aiiy EIN ? ?o ie?aieo
no?oeoo?ieo eiiiiiaio(a ooai?a R. solanacearum, ui a?ae??ciyeeny
i?eiaeaaei?noth aei iaaiiai a?iaa?o ? ye? aoei aeeo/aii ?c ??cieo ?ineei
oa ?(cieo aaia?ao(/ieo cii na(oo.

Anoaiiaeaii, ui I-IN R. solanacearum i?aaenoaaeai? o?e-, oao?a- oa
iaioanaoa?eaeieie e?i?eieie aai ?icaaeoaeaieie eaioethaaie, ui
iiaoi?ththoueny. Aa??aoe?? a ?o aoaeia? aeaee ciiao ?iciiae?eeoe I-IN
aeine?aeaeoaaieo ooai?a ia 7 no?oeoo?ieo oei?a , 6 c yeeo iienai?
aia?oa.

C’yniaaii, ui a?eueo?noue aeine?aeaeoaaieo ooai?a R. solanacearum
aaoa-?iaaiia ca neeaaeii EIN, ye? i?noyoue a?eueoa i?ae iaeei oei
no?oeoo?e I-IN.

Aeey aeaeo R. solanacearum aia?oa i?iaaaeai? ii??aiyeuei? aeine?aeaeaiiy
caeaaeiino? na?ieia?/ii? aeoeaiino? e?iiiie?naoa?eae?a a?ae no?oeoo?ieo
iniaeeainoae ?o I-IN ? aecia/aii, ye? c ieo ? nooo?aeie a i?iya? oe???
aeoeaiino?.

Aia?oa iieacaia caeaoi?noue e?iiiie?naoa?eaeo R. solanacearum ? eiai
ie?aieo no?oeoo?ieo eiiiiiaio?a i?iyaeyoe ?ioa?oa?iiiaaiio oa
aioeiaoanoaoe/io ae?th.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a

Iaea?aeai? aeai? i?i o(i(/iee neeaae ? iniaeeaino( aoaeiae EIN oa ?o
ie?ai? no?oeoo?i? eiiiiiaioe iiaeooue aooe aeei?enoai? aeey ae?(oaiiy
iac’yniaaieo ieoaiue a?ieia?? oa nenoaiaoeee aeaeo R. solanacearum , a
naia, i?e aeine?aeaeaiiyo, i?enay/aieo ooi/iaiith aioo??oiueiaeaeiaiai
oaeniiii?/iiai noaio i?aaenoaaiee(a oeueiai aaoa?iaaiiiai aeaeo, a
oaeiae i?e aeine?aeaeaiiyo o?c(ieia?? oa iaoai?ci?a iaoiaaiaco oai?iae
?ineei, yeo a?i aeeeeea?. I?iaaaeai? na?ieia?/i? aeine?aeaeaiiy
yaeythoueny ia?aaeoiiaith aeey iiaeaeueoeo ?ia?o, iai?aaeaieo ia
noai?aiiy ia iniia? I-aioeaaiiino? e?iiiie?naoa?eae?a na?ieia?/ii?
eeaneo?eaoe?eii? noaie aeaeo R. solanacearum, ui aeanoue ciiao aeey
?aeaioeo?eaoe?? ooai?a oeueiai o?oiiaoiaaio.

A(aeiiino(, iaea?aeai( i?e aea/aii( aieeao EIN R. solanacearum oa ?o
ie?aieo no?oeoo?ieo eiiiiiaio(a ia (ioiio nenoaio iae?ii?aai(cio
iaiao?aei? i?e ?ic?iaoe( i?aia?ao(a iai?aaeaii( ae(( ia ?(ci( eaioethae
(ioi(oaoo.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe?? . Iniiai( ?acoeueoaoe ( iieiaeaiiy
aeena?oaoe(( aeiiia?aeaeenue oa iaaiai?thaaeenue ia Ananithci?e
oeie?-eiioa?aioe?? iieiaeeo a/aieo (Aeoooa, 1990); eiioa?aioe??
ooiaeaiaioaeueieo aeine?aeaeaiue (inoeoooo i(e?ia(ieia(( ? a(?onieia((
(i. Caaieioiiai (Ee?a, 1993); I Onoaiia-/iio c’?cae? Oe?a?inueeiai
i?e?ia?ieia?/iiai oiaa?enoaa (Iaeana, 1993); XVII Iiaeia?iaeiiio
neiiic(oi( ii aoaeaaiaeai (Iooaaa, 1994); 5-o(e I(aeia?iaei(e
eiioa?aioe(( c i?iaeai iaoiaa?o Pseudomonas syringae oa nii?(aeiaieo
iaoiaai(a (Aa?e(i, 1995); (a?iiaenueeiio neiiic(oi( c aoaeaaiae(a
“Eurocarb-9” ( Aieaiae(y, 1997).

Ioae(eaoe((. Ca iaoa?(aeaie aeena?oaoe(( iioae(eiaaii 9 noaoae, c yeeo
2 — a i(aeia?iaeieo aeo?iaeao, 3 — a aeo?iaeao ?in(enueei( aeaaeai((
iaoe, 4 — a aeo?iaeao Iaoe(iiaeueii( aeaaeai(( iaoe Oe?a(ie.

Iniaenoee aianie caeiaoaa/a. Iniiai( aenia?eiaioaeuei( aeai( aoee
iaea?aeai( caeiaoaa/ai iniaenoi: iaea?aeaii aaeoa?(aeuei( iane 17
aeine?aeaeoaaieo ooai?a Ralstonia solanacearum, c yeeo aeae(eaii EIN oa
(o ie?ai( no?oeoo?i( eiiiiiaioe: I-IN, ei?-IA oa e(i(aee A. I?iaaaeaii
aecia/aiiy iiiinaoa?eaeiiai, ai(iieeneioiiai oa aee?iieeneioiiai neeaaeo
iaea?aeaieo EIN oa (o ie?aieo eiiiiiaio(a. Iaoeethaaiiy, ia?eiaeaoia
ieeneaiiy, aeacaoeaoeethaaiiy, aeacai(ioaaiiy, ?iciaae ca Ni?oii,
anoaiiaeaiiy eiio?ao?aoe?? iiiinaoa?eae?a i?iaaaeaii iniaenoi.
Anoaiiaeaiiy no?oeoo? I-IN aeine(aeaeoaaieo ooai(a i?iaaaeaii ni(eueii
c ae.o.i. Ei??aeue TH.I., ae.o.i. Oaoeiaei I.I. oa e.o.i. Ei/a?iaith
I.I. (?inoeooo i?aai?/ii? o?i?? ?i. Caeeinueeiai, Iineaa).
(iioiio?i?/i? aeine?aeaeaiiy i?iaaaeaii iniaenoi, aaoi? aeneiaeth(
iiaeyeo e.a.i.Eaaoi A.I. ((inoeooo a(io(i(( (i. Iaeeaae(ia, Ee(a) ca
aeiiiiiao a ?ic?iaoe( oiia i?iaaaeaiiy (ioiioa?iaioiiai aiae(co aeey
aeine(aeaeoaaieo i?aia?ao(a. Anoaiiaeaiiy (ioa?oa?iiiaaiii( aeoeaiino(
EIN cae(eniaii ni(eueii c ae.i.i. N.E.?eaaeei oa e.i.i. Aeyaethi N.O.
((inoeooo ai(aeai(ieia(( oa (ioaeoe(eieo oai?ia (i. A?iiaoaanueeiai,
Ee(a); aecia/aiiy i?ioeiooeeiii( ae(( EIN i?iaaaeaii ca o/anoth e.a.i.
I. A. I?eaeaoei oa ?iae. AEoeiai( I.A. ((inoeooo aenia?eiaioaeueii(
iaoieia((, iieieia(( oa ?aae(ieia(( IAI Oe?a(ie, Ee(a). Aaoi? aeneiaeth?
ue?o iiaeyeo e.a.i. Io?an A.I. oa (iae. AEeoeaa(/ I. A. ((inoeooo
i(e?ia(ieia(( oa a(?onieia(( (i. Caaieioiiai) ca aeiiiiiao o i(aeo?eioe(
i?e?ia?ieia?/ii “/enoi(“ eoeueoo?e aeine(aeaeoaaieo ooai(a.

No?oeoo?a oa ia’(i ?iaioe. Aeena?oaoe(y neeaaea(oueny c anooio, iaeyaeo
e(oa?aoo?e, iieno iaoa?(ae(a oa iaoiae(a aeine(aeaeaiiy,
aenia?eiaioaeueii( /anoeie, yea aeeth/a( aeeeaae o /ioe?ueio ?icae(eao
?acoeueoao(a ?iaioe oa (o iaaiai?aiiy, aeniiae(a, a oaeiae nieneo
e(oa?aoo?e, yeee ioiieth( 139 ioae(eaoe(e. ?iaioa aeeeaaeaia ia 145
noi?(ieao iaoeiiieniiai oaenoo. Oaeoe/iee iaoa?(ae aeena?oaoe(( iiaeaii
o aeaeyae( 31 ?enoie(a oa 29 oaaeeoeue.

?ICAe?E 1. IAEssAe E?OA?AOO?E

O aeaio i?ae?icae?eao aeeeaaeaii a?aeiiino? i?i a?io?i?/i? aeanoeaino?
oa o?oiiaoiaaiio ae?th i?aaenoaaiee?a aeaeo R. solanacearum; i?i
no/aniee noai oaeniiii?/iiai iieiaeaiiy oeueiai aeaeo, a nenoaiaoeoe?
yeiai ia i?ioyc? inoaii?o ?ie?a a?aeaoeinue aeae?eueea ci?i; i?i
neeaae ? aoaeiao EIN o?oiiaoiaaiieo aaeoa??e ?iaeei Ralstonia,
Burkholderia, Pseudomonas.

?ICAe?E 2.IAOA?(AEE OA IAOIAeE

Ia’(eoaie aeino(aeaeaiiy aoee 17 ooai(a Ralstonia solanacearum. An?
eoeueoo?e aoee etha’ycii iaaeai? eo?aoi?ii ICMP aeieoi?ii Aeae.
ssiaii (Iiaa Caeaiae?y), ca ui ie aeneiaeth?ii eiio ue?o iiaeyeo. Ooaie
aoee aeae?eai( a ?(cieo aaia?ao(/ieo ciiao na(oo ?c ??cieo ?ineei ? ca
nai(ie aeanoeainoyie a?aeianai( aei ??cieo a?iaa??a (oaae. 1).

Eoeueoeaoaaiiy aaeoa?(e i?iaiaeeee ia ?(aeeiio neioaoe/iiio na?aaeiaeu(,
ui i(noeei ye aeaea?aei aoaeaoeth aetheico, a acioo — oei?enoee aiii(e
(Vidaver).

Aeae(eaiiy oa i/euaiiy EIN i?iaiaeeee ca iaoiaeii Aanooaey oa ssiia.
Aeaa?aaeaoe(th EIN i?iaiaeeee i’yeei eeneioiei a(ae?ie(cii a 1%
ioeoia(e eeneio(. Oeueo?aoeaio?eooaoaaiiyi (40 oa., 25000 g) a(aeae(eyee
a(ae?io(euei( oa a(ae?ioiai( /anoeie aeaa?aaeiaaiiai EIN. Ie?ai(
aoaeaaiaei( eiiiiiaioe iieaeoee EIN iaea?aeoaaee aaeue-o?iiaoia?ao((th
ia naoaaeaen( G-50 c aeei?enoaiiyi 0.025I i(?eaeei-aoeaoaoiiai aooa?o,
?I 4,5. Aecia/aiiy e(eueeino( aoaeaaiae(a cae(enithaaee ca Dubois,
ioeea(iiaeo eeneio — ca Ni(?(iei,

Oaaeeoey 1

Ooai A?iaa? ?ineeia-oacy?i

ICMP 5712 — oeiiaee (NCPPB 325, ATCC 11696) 1 oiiaoe

ICMP 766 1 oiiaoe

ICMP 767 1 aaiai

ICMP 6523 1 othothi

ICMP 7942 1 aaiai

ICMP 7944 1 iiaei?iaeiee

ICMP 7955 3 aaeeaaeai

ICMP 7956 2 oiiaoe

ICMP 7959 2 nieiaeeee ia?aoeue

ICMP 7960 4 nieiaeeee ia?aoeue

ICMP 8066 1 othothi

ICMP 8072 1 othothi

ICMP 8089 3 nieiaeeee ia?aoeue

ICMP 8093 1 oiiaoe

ICMP 8110 3 ea?oiiey

ICMP 8202 4 ea?oiiey

ICMP 8277 4 nieiaeeee ia?aoeue

a(eeo — ca Lowry. Aaioice aecia/aee ?aaeoe((th c oeenoa(iii oa n(?/aiith
eeneioith, EAeI — ?aaeoe((th c o(iaa?aeoo?iaith eeneioith.
(aeaioeo(eaoe(th iaeo?aeueieo iiiinaoa?eae(a cae(enithaaee iaoiaeii
aaciai-?(aeeiii( o?iiaoia?ao(( i?aia?ao(a o aeaeyae( aoeaoao(a iie(ie(a
ia aaciaiio o?iiaoia?ao( “Chrom-5”. (aeaioeo(eaoe(th oa aecia/aiiy
ai(noo ai(iieeneio oa aaenicai?i?a i?iaiaeeee ia aiae(caoi?( ai(iieeneio
“Hitachi”. Aecia/aiiy aee?iieeneioiiai neeaaeo e(i(ae(a A i?iaiaeeee ia
aaciaiio o?iiaoia?ao( “Oeaao-100”, aiae(coth/e i?aia?aoe o aeaeyae(
iaoeeiaeo ao(??a aee?ieo eeneio, iayai(noue ieneeeneio i(aeoaa?aeaeoaaee
o?eooi?aoeaoeethaaiiyi.

No?oeoo?e I-IN anoaiiaethaaee c canoiniaoaaiiyi aacaeano?oeoeaieo (1I-
oa 13N-ssI?-niaeo?ineii?y) oa aeano?oeoeaieo (ia?eiaeaoia ieeneaiiy,
?iciaae ca Ni?oii, iaoeethaaiiy, aeacaoeaoeethaaiiy, aeacai?ioaaiiy)
iaoiae(a, a oaeiae ca aeiiiiiaith iaoiaea eiii’thoa?iiai aiae?co
(Lipkind et al.).

(ioiio(i(/i( aeanoeaino( EIN aea/aee canoiniaoth/e iaoiae oaa?aeioaciiai
(ioiioa?iaioiiai aiae(co.

(ioa?oa?ii(iaeoeoth/o aeoeai(noue anoaiiaethaaee a aeine(aeao in vitro.
(iaeoeoe(th (ioa?oa?iia i?iaiaeeee oeyoii aianaiiy a noniaic(th
eaeeioeeo(a ia?eoa?(eii( e?ia( ethaeeie ( 5o106 ee(oei ia 1 ie)
iie(naoa?eaea a e(ioeaa(e eiioeaio?aoe(( 200 iea a i?ia(. Aeoeai(noue
(ioa?oa?iio aecia/aee ca caeaoi(noth i?eai(/oaaoe oeeoioiene/io ae(th
100 oeaieiieo oeeoiieaciaoe/ieo aeic (OOeAe) a 1 ie a(?ona-(iaeeeaoi?a
(a(?on aaceeoey?iiai noiiaoeoo). Ca iaeeieoeth aeoeaiino( (ioa?oa?iio
((I50/ie) i?eeiaee cia/aiiy, iaa?iaia eiai iaeneiaeueiiio ?icaaaeaiith,
ui caiia(aaei ia 50% oeeoiiaoe/i(e ae(( 100 OOeAe AAN a eoeueoo?(
eaeeioeeo(a ethaeeie e(i(( L41, ui ia?aaeaaeeny.

Aeine(aeaeaiiy i?ioeiooeeiii( aeoeaiino( i?iaiaeeee ia i’yoe iooeeiieo
ooaiao: iaoanoacoth/a ea?oeeiiia eaaai(a Eueth(n (3LL) oa iaeaiiia A-16;
anoeeoia na?eiia S-37 (iaaeeoa?eioe(eiaaia iie(ii?oiiee(oeiia na?eiia) (
aeaa eaeeicieo ooaie — e(ioi(aeia eaeeai(y L1210 ( e(ioioeeoa?ia
eaeeai(y ?-388. I?ioeiooeeiiee aoaeo ioe(ithaaee ii (ioaineaiino( ?inoo
cia/ieo iooeei (aecia/aiiy (o ?ici(?o) ( e(eueeino( ee(oei a anoeeoe/i(e
?(aeei( i?e anoeeoi(e oi?i( ?inoo ( na?eiia S-37). Aioeeaeeicio
aeoeai(noue aecia/aee ia i(aenoaa( i(ae?aooieo caaaeueii( e(euee(no(
ee(oei a anoeeoe/i(e ?(aeei( ( ca o?eaae(noth aeeooy oaa?ei. sse
iieaciee aioeiaoanoaoe/ii( aeoeaiino( aeine(aeaeoaaee na?aaei(e noia?iee
ia’(i iaoanoac(a ( (o e(euee(noue ia iaeio oaa?eio.

?acoeueoaoe aeine?ae?a noaoenoe/ii ia?iaeyee, aeei?enoiaoth/e
eiao(oeeaio No’thaeaioa.

?ICAe?E 3. AEAe(EAIIss E(IIIIE(NAOA?EAe(A R. solanacearum , AECIA*AIIss
(O EIIIIIAIOIIAI NEEAAeO

?c 17 aeine?aeaeoaaieo ooai?a R. solanacearum iaea?aeai? aaeoa??aeuei?
iane, c yeeo o iiaeaeueoiio aeae?eai( EIN. C’yniaaii, ui aeey
i?aaenoaaiee?a aeaeo R. solanacearum, a?eueoa /anoeia EIN (a?ae ?o
noia?iiai ai?noo) aeno?a-ao?oueny o aiaeiee oa? aiaeii-oaiieueii(
noi(o( : 70-85% (o oaiieuei?e o?ae-oe?? aeyaeyee 30-15%). Oaea
?iciiae?eaiiy i?ae o?aeoe?yie ?aaeoe(eii( noiio( aeaco(, ui aei neeaaeo
aeine?aeaeoaaieo EIN aoiaeyoue ia?aaaaeii a?ae?io?euei? eii-iiiaioe. EIN
?c oaiieueii? o?aeoe?? iaaaaaoi a?eueoa caa?oaeiai? ioeea?ii-aeie
eeneioaie, i?ae EIN ?c aiaeiiai oa?o (a?aeiia?aeii, 40-50 oa
20-35%). Ca ye(niei neeaaeii EIN ?c oaiieueii? oa aiaeii? o?aeoe?e
ia a(ae?(ciyeenue.

Anoaiiaeaii, ui ooaie aeaiiai aeaeo a?ae??ciythoueny i?ae niaith
a?aeiiniei ai?noii EIN: oi/a aeno?aeoe?y EIN an?o aeine?aeaeoaaieo
ooai?a a?aeaoaaeanue ca iaeiaeiaeo oiia, (o e?ioeaaee aeo?ae ii
a?aeiioaiith aei nooi? aaeoa??aeueii? iane i?ae ie?aieie ooaiaie
eieeaaany a?ae 1,2 % o R. solanacearum oo. 7942 aei 15,4 % o oo.
7956, aeey a?eueoino? ae ooai?a a?i noaiiaea 5-10%. O?aaa
a(aecia/eoe, ui ca a(aeiiniei ai(noii EIN i?aaenoaa-ieee aeaeo R.
solanacearum aeae(eueea a(ae?(ciythoueny a(ae (ioeo a?aiiaaaoeaieo
aaeoa?(e, aeey yeeo ai(no oeeo niieoe neeaaea( a na?aaeiueiio a(ae 1 aei
5 %.

EIN an(o aeine(aeaeoaaieo ooai(a aoee a?euei? a?ae aeii?oie a?eeo,
ai?no yeiai aecia/aany ne?aeiaeie e?eueeinoyie. Aea an( EIN
oa?aeoa?e-coaaeeny aeinoaoiuei aenieei ai?noii ioeea?iiaeo eeneio
(aei 35 %), iicaaa-eyeeny a(ae yeeo canoiniaoth/e aeae?eueea oeeee?a
oeueo?aoeaio?eooaoaaiiy, i?e oeueiio (o ai?no ciaiooaaany aei 1,5-5 %.
Iaeiae aeey EIN aeayeeo ooai?a, cac-ia/aiee iaoiae aeyaeany
iaaeinoaoiuei aoaeoeaiei — i?ney oeueo?aoeaio?eooao-aaiiy ai?no
ioeea?iiaeo eeneio caeeoaany aeineoue aenieei (a(ae 6 aei 13,5 %).
Aeey EIN oeeo ooai?a i?iaiaeeee iiaeaeueoa i/euaiiy ca aeiiiiiaith
iane/aiiai ?ic/eio o?eoei?ioeoiai? eeneioe. Aeyaeaii, ui oeae iaoiae
i/euaiiy iaaaaaoi ae?eia?oee (ai?no ioeea?iiaeo eeneio ciaiooaaany aei
0, 5 — 2 %), iaeiae i?e eiai canoinoaaii? caeoee e?eueeino? EIN cia/ii
a?eueo?.

Aiae?c iiiinaoa?eaeiiai neeaaeo EIN iieacaa, ui aeii?ioth/ei
iiiinaoa?eaeii an?o EIN ? ?aiiica (70 — 90 %). EIN i?aeoe/ii an?o
ooai?a iaee aetheico (1 -23 %), ca oeei iieacieeii aeae?eueea aeae?eyany
na?aae ?ioeo oo. 7956 (35 %). Oa?aeoa?iith aeyaeeanue oaeiae eneeica
(1-8 %) . EIN aeayeeo ooai?a iaee iaio iioe?ai? aeey aeaiiai aeaeo
aoaeaaiaei? caeeoee: ?eaico, aaeaeoico, iaiico oa a?aa(iico. Ai(no
aaioic, a caeaaeiino? a?ae ooaio, neeaaeaa a?ae 0,5 aei 3,0 %. Aei
neeaaeo an?o EIN aoiaeea a aeinoaoiuei cia/i?e e(eueeino( aetheicai?i
(4-18 %), a EIN aeaio ooai(a (oo. 7944 oa 8089) aecia/aii ua ?
aaeaeoicai?i.

Uiaei ai?iieeneioiiai neeaaeo EIN, oi oe? niieoee yaeythoueny
i?ii?ieie eiiiiiaioaie. Ca ye?niei neeaaeii ea?oeia (o ?iciiae?eaiiy
aeineoue ??ciiiai?oia: i?aaenoaaeai? iaeaea an? ai?iieeneioe (ca
aeeeth/aiiyi eaeoeeio oa oe?iceio), aea iaea?eueo iioe?aieie aeyaeeenue
aeai?i oa aenoeaeei, c ia?aaaaith aeai?io o e?euee?niiio a?aeiioaii?.

Aeaa?aaeaoe((th ( iiaeaeueoith aaeue-o?iiaoia?ao?(th aoaeaaiaeii(
/anoeie aeaa?aaeiaaiiai EIN io?eiai? ie?ai( no?oeoo?i( eiiiiiaioe
iieaeoee. EIN an?o aeine?aeaeoaaieo ooai?a ca nai?th no?oeoo?ith
iaeaaeaoue aei S-oi?ie – neeaaeathoueny ?c I-niaoeeo?/iiai
iie?naoa?eaeo, ie?ainaoa?eaeo ei?o oa e?i?aeo A. Aeey a?eueoino? ooai?a
aoei oa?aeoa?ii ?icae?eaiiy aoaeaaiaeii? /anoeie ia o?e i?ee (I-IN oa
ei?-IA) — ?en. 1. Iniaeea?noth ae?oai? a?oie, ui i?aaenoaaeaia 4
ooaiaie (oo. 766, 8072, 8110 oa 8202), aoea iayai?noue ua iaei???,
a?eueo aenieiiieaeoey?ii? o?aeoe?? ( o e?euee?niiio a?aeiioaii? cia/ii
iaioi( — eeoa 3-8 % a?ae o?aeoe?? I-IN) — ?en. 2. Ca ye?niei neeaaeii
I-IN ? I’-IN i?aeoe/ii ia a?ae??ciyeenue, ??cieoey aoea iaoiiaeaia, ye
i?aaeei, a?eueoei ai?noii aetheice o neeaae? I’-IN.

?en. 1 I?io?eue aethoe?? aeaa?aaeiaaiiai EIN R.solanacearum oo. 5712

?en. 2 I?io?eue aethoe?? aeaa?aaeiaaiiai EIN R.solanacearum oo. 8110

?ICAe?E 4. NO?OEOO?I( EIIIIIAIOE E?IIIIE?NAOA?EAe?A

I-niaoeeo(/i( iie(naoa?eaee

Aeii?ioth/ei iiiinaoa?eaeii I-IN aeine?aeaeoaaieo ooai?a aoea ?aiiica
(60 — 93%). Aeey aaaaoueio ooai?a oa?aeoa?ieie aoee oaeiae eneeica (3 —
12 %) oa aetheica (2-19%). I-IN aeayeeo ooai?a i?noeee iaaaeeeo
e?euee?noue a?aa?iice (1-6 %), a oaeiae aaeaeoice (1-10%) ? eeoa I-IN
oo. 8089 a?ae??ciyany a?ae ?ioeo cia/iei ai?noii inoaiiuei? — 31,6 %.

Aeey an(o I-IN aecia/aii cia/io e?euee?noue aetheicai?io (5-20%). Aei
neeaaeo I-IN o?ueio ooai?a (oo. 7944, 7955 oa 8089) aoiaeea oaeiae
aaeaeoicai?i. O nai?e a?eueoino? aeine?aeaeoaai? I-IN aoee a?euei?
a?ae ai?iieeneio ? eeoa I-IN ie?aieo ooai(a i?noeee iaeio /e aea(
ai?iieeneioe a aeoaea iecueeeo eiioeaio?aoe?yo (0,1-0,7%), i?e/iio,
ia?aaaaa, ye ? a ?ac? EIN, aoea ca aeai?iii oa aenoeaeeiii.

Aeoiaey/e c aiae(co 1I- oa 13N-ssI?-niaeo?ineii?( anoaiiaeaii, ui I-IN
aeine(aeaeoaaieo ooai(a neeaaeathoueny (c o?e- oao?a- oa
iaioanaoa?eaeieo e?i?eieo aai ?icaaeoaeaieo eaioetha(a, ui
iiaoi?ththoueny (?en. 3).

?en. 3 13N-ssI?-niaeo?e I-IN R.solanacearum.

C canoiniaoaaiiyi aeano?oeoeaieo iaoiae(a, ye( aeicaieeee anoaiiaeoe oei
cai(uaiiy, iieiaeaiiy ( eiio(ao?aoe(th ae?eiceaeieo ca’yce(a ie?aieo
iiiinaoa?eaeieo caeeoe(a, a oaeiae ca aeiiiiiaith iaoiaea eiii’thoa?iiai
aiae?co aoei anoaiiaeaii 7 oei?a no?oeoo? I-IN, 6 c yeeo iienai? aia?oa
(oaae. 2).

Oaaeeoey 2

?iciiae?eaiiy oei?a no?oeoo? eaioethaa, ui iiaoi?th?oueny

I-IN aeine(aeaeoaaieo ooai?a R. solanacearum

no?oeoo?a

Aoaeiaa eaioethaa, ui iiaoi?th(oueny Ooai Iayai(noue oei(a no?oeoo?
Ni(aa(aeiioaiiy oei(a no?oeo. o %

6523

1 (3)-(-D-GlcpNAc-(1(2)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)-(-L-Rhap-(1( 7956
1 100

A B
C D 8110

8202

767

2 (3)-(-D-GlcpNAc-(1(3)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)-(-L-Rhap-(1(

A B
C D 7942 2 100

3

(3)-(-D-GlcpNAc-(1(3)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)- (-L-Rhap-(1(

A B
C (4

(1

(-L-Xylp 5712

766

7959

8066 3 : 2 70 : 30

E 7960

25 : 75

8093

4

(3)- (-D-GlcpNAc-(1(3)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)-(-L-Rhap-(1(

A B
C D

7955

4: (3+2)

70 : 30

5

(3)-(-D-GlcpNAc-(1(2)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)- (-L-Rhap-(1(

A B
C (4

(1

(-L-Xylp

E

8072

5 : 1 : 3

40:50:10

6

(-L-Rhap E

(1

(3

(3)-(-D-GlcpNAc-(1(2)-(-L-Rhap-(1(2)-(-L-Rhap-(1(3)- (-L-Rhap-(1(

A B
C D

7944

8277

6 : 1

40:60

7

(3)-(-D-Rhap-(1(3)-(-D-Rhap-(1(4)-(-D-GalpNAc-(1(

8089

7

100

Na?aae aeine(aeaeoaaieo ooai(a cia/ii a?ae??ciyany a?ae ?ioeo I-IN oo.
8089: a?i iiaoaeiaai ?c e?i?eieo o?enaoa?eaeieo eaioetha?a, ye?
aeeth/athoue 2 caeeoee D-?aiiice oa 1 caeeoie N-aoeaoeeaaeaeoicai(io.

I-IN 7 ooai?a oa?aeoa?ecoaaeenue e?i?eiith no?oeoo?ith c
oao?anaoa?eaeieie eaioethaaie, ui iiaoi?ththoueny , ye? neeaaeaeenue ?c
o?ueio caeeoe?a L-?aiiice oa iaeiiai caeeoea N-aoeaoeeaetheicai(io. Aiie
a?ae??ciyeenue i?ae niaith aa??aoe?yie o oei? eiio?ao?aoe??
ae?eiceaeiiai ca’yceo caeeoeo N-aoeaoeeaetheicai(io : ( — a no?oeoo?ao 1
( 4 oa ( — a no?oeoo?( 2, a oaeiae iiceoe??th eiai i?ee??ieaiiy aei
caeeoeo ?aiiice : 1(2 a no?oeoo?( 1 oa 1(3 a no?oeoo?ao 2 ( 4. I-IN ?c
no?oeoo?ith 1 aoei aecia/aii oaeiae yiiinueeeie aeine?aeieeaie Ae?yia c
ni?aaao. (1985) ? ia ii/aoie iaoeo aeine?aeaeaiue oea aoea ?aeeia
a?aeiia no?oeoo?a I-IN i?aaenoaaiee?a oeueiai aeaeo.

Aeey a?eueoino? ooai?a aoei aecia/aii iayai?noue ?icaaeoaeaieo
iaioanaoa?eaeieo eaioetha?a, iniiao yeeo neeaaeaee e?i?ei?
oao?anaoa?eaei? no?oeoo?e 1 /e 2. ??cieoey noinoaaeanue oeio a?/iiai
cai?nieea: eneeica a no?oeoo?ao 3 oa 5 /e ?aiiica a no?oeoo?( 6, a
oaeiae i?noey eiai i?ee??ieaiiy aei aieiaiiai eaioethaa. O?aaa
a?aei?oeoe, ui eneeica yaey?oueny ??aee?niei eiiiiiaioii aaeoa??aeueieo
IN ? ia oeae /an a?aeiia ye neeaaeiaa I-IN eeoa o P. maltophylia .

Aeey aaaaoueio ooai?a aoei aecia/aii iayai?noue a neeaae? I-IN
aeae?eueeio oei?a no?oeoo?, i?e/iio ?iiae? ??cieoey i?ae ooaiaie
noinoaaeanue eeoa ni?aa?aeiioaiiy oei?a no?oeoo? (aeea. oaae. 1, a?oia
no?oeoo?e 3). Iiae(aia aaoa?iaaii(noue aoea anoaiiaeaia oaeiae ?ai(oa
Caei?iaaiei c ni(aaao. i?e aea/aii( Pseudomonas syringae (1988-1991) oa
Nieaeaoe(iith (1989) i?e aea/aii( I-IN Burkholderia cepacia .

Ca ye?niei neeaaeii I-IN aeine?aeaeoaai? ooaie iiaeia ?iciiae?eeoe ia 3
oaiioeie: i?aaenoaaieee I iaee o neeaae? I-IN L-?aiiico oa
N-aoeaoeeaetheicai(i; II — e?(i oiai ua ? eneeico; III — D-?aiiico oa
N-aoeaoeeaaeaeoicai(i.

Ei?aeyoe?? i?ae no?oeoo?ith I-IN ? iaeaaei?noth ooaio aei iaaiiai
a?iaa?o /e ?ineeie-oacy?ia aai aaia?ao(/ii( ciie, c yei? oeae ooai
iioiaeeoue, ia aeyaeeinue.

Ei? -ie(ainaoa?eaee.

Oi/a a?aeiii, ui ei?-IA ? aeineoue eiina?aaoeaiith /anoeiith iieaeoee
EIN, aeey aeine(aeaeoaaieo ooai(a aoei aeyaeaii cia/i? aa??aoe?? uiaei
ai?noo ie?aieo eiiiiiaio?a. Iaea?eueo iioe?aieie aoee ?aiiica oa
aetheica. Aea (o ai?no eieeaaany a aeoaea oe?ieeo ae?aiaciiao :
?aiiice — a?ae 1 aei 81,3 %; aetheice — a?ae 2 aei 98 %. E??i oeeo
aoaeaaiae?a o ei?-IA a?eueoino? ooai?a aeyaeaii ?yae ?ioeo
iiiinaoa?eae?a: ii-ia?oa ne?ae a?aei?oeoe aaeaeoico, i?e/iio, aeey aeaio
ooai(a (oo. 8066 oa 8072) aiia aoea, ?acii ?c ?aiiicith, aeii?ioth/ei
iiiinaoa?eaeii, a oie /an, ye aetheica a ieo aecia/aeanue a iacia/i(e
e?eueeino? (oo. 8072), /e aoea iiai(noth a?aenooiy (oo. 8066). Ei?-IA
?yaeo ooai?a iaee o nai?io neeaae? eneeico oa ?eaico. Aecia/aiiy ?eaice
a ei?-IA iiynith( (( (aeaioeo(eaoe(th i?e aiae(c( EIN. Iacae/aeia
ea?oeia iiiinaoa?eaeiiai neeaaeo niinoa??aaeanue aeey ei?-IA oo.7942: 39
% iaiice ? 31 % a?eo?eoo.

Oe?eaaei aeyaeany ai?no a ei?-IA aeine?aeaeoaaieo ooai?a ai?iiniieoe.
E??i aetheicai?io, cae/aeiiai eiiiiiaioo ei?-ie?ainaoa?eae?a aaaaoueio
aeae(a aaeoa?(e, aei neeaaeo ei?-IA a?eueoino? ooai?a aoiaeea a aeineoue
aenieeo eiioeaio?aoe?yo aoaiieai?i — 0,5- 2,5%. Ei?-IA an(o ooai(a
oa?aeoa?ecoaaeenue aeineoue iino?eiei iaai?ii ai?iieeneio, a naia,
aeai?io, ae?oeeio oa aeooai?iiai( eeneioe.

E(i(aee A.

sse a(aeiii, e(i(ae A yaey(oueny iaea?eueo eiina?aaoeaiith a i?ioean(
aaiethoe(( /anoeiith iieaeoee EIN. E(i(aee A ?(cieo aeae(a aaeoa?(e
a(ae?(ciythoueny i(ae niaith, a ia?oo /a?ao, neeaaeii aee?ieo eeneio,
yeee ( aeineoue noaa(eueiei iieacieeii ( aeei?enoiao(oueny ye iaeei (c
oaiioaeniiii(/ieo e?eoa?((a.

Aeey i?aaenoaaiee(a aeaeo R. solanacearum a neeaae( e(i(ae(a A
ia?aaaaeaee aee?i( eeneioe c ia?iei /eneia aoii(a aoaeaoeth, a naia
i’youe aeii(ioth/eo eiiiiiaio(a: i-oao?a-, i-aaenaaeaeaiiaa eeneioe, a
oaeiae 3-ieneoao?a-, 3-ieneaaena- oa 3-ieneieoaaeaeaiiaa eeneioe.
Iayai(noue 3-II-N14:0 oa 3-II-N16:0 o neeaae( e(i(ae(a A (
oa?aeoa?iith iciaeith i?aaenoaaiee(a II ??IE-aiiieia(/ii( a?oie ?iaeeie
Pseudomonadaceae, aei yei( iaeaaeaa aei iaaeaaiueiai /ano ?(ae
Ralstonia. Iayai?noue 3-ieneieoaaeaeaiiai( eeneioe iiaeeeai ( iaei((th
c niaoeeo(/ieo iciae aeaeo R.solanacearum.

?ICAe?E 5. A(IEIA(*IA AEOEAI(NOUe E(IIIIE(NAOA?EAe(A

(ioiio(i(/i( aeanoeaino(

A(aeiii, ui aa?(aoe(( a no?oeoo?( I-IN eaaeaoue a iniia( na?ieia(/ieo
eeaneo(eaoe(eieo noai. Aeey aeaeo R. solanacearum ia nueiaiaei( oaei(
noaie ia (nio(. Oiio aoei i?iaaaeaii aeine(aeaeaiiy c iaoith
anoaiiaeaiiy iiaeeeai( ei?aeyoe(( i(ae na?ieia(/iith aeoeai(noth EIN oa
no?oeoo?ith eiai I-IN, a oaeiae ni?iae c’ynoaaiiy aioeaaiii(
aeaoa?i(iaioe.

Na?ieia?/io aeoeai?noue EIN aea/aee ca aeiiiiiaith aioene?iaaoee,
io?eiaii? aei a??oi? aaeoa??aeueii? noniaic?? R. solanacearum oo.
8110, EIN yeiai oa?aeoa?ecoaaany iayai?noth o?eueee iaeiiai oeio
no?oeoo?e I-IN. Oeae EIN iieacaa aenieo na?ieia?/io aeoeai?noue o
a?aeiioaii? aei cacia/aii? aioene?iaaoee ( aeei?enoiaoaaany ye
iiceoeaiee eiio?ieue, ii??aiaiiyi c yeei ioe?ithaaeanue aeoeai?noue
?ioeo aioeaai(a. sse iaaaoeaiee eiio?ieue aoei aeei?enoaii EIN oo. 4157,
I-IN yeiai ca nai?th no?oeoo?ith i?eioeeiiai a?ae??ciyany a?ae
aeine(aeaeoaaieo:

( 6)-(-D-GlcNAc-(1( 4)-(-D-GalNAc-(1(

(4

(1

(-L-Araf

( yeee aeyaeany na?ieia?/ii ?ia?oiei aei aioene?iaaoee.

Ca na?ieia(/iith aeoeai(noth aeine(aeaeoaai( EIN, a(ae?(ciyeanue i(ae
niaith. Ia ?en. 4 a(aeia?aaeaii ?iciiae?eaiiy EIN aeine?aeaeoaaieo
ooai?a ca no?oeoo?iei oeiii I-IN oa “a?aeiinio” na?ieia?/io
aeoeai?noue, yeo aiie i?iyaeee i?e cacia/aieo oiiaao oanooaaiiy. (c
aiae(co oe((( ae(aa?aie aeo(ea(:

1. EIN, a no?oeoo?( yeeo N-aoeaoeeaetheicai(i ca’ycai c caeeoeaie
?aiiice (- (1(2)-ca’yceaie (oo. 8110, 6523, 7956, 8202) yaeythoueny
na?ieia(/ii aeoeaieie, a oie /an, ye EIN (c (- (1(3)- ca’yceaie (oo.
767, 7942) — na?ieia(/ii iia?oi( a ?aaeoei( c aeei?enoaiith a
aeine(aeaeaii( aioene?iaaoeith.

2. ?icaaeoaeaiiy eaioethaa e(i(eii( no?oeoo?e ( iiyaa caeeoeo L-eneeice
ye a(/iiai cai(nieea caeeoeo ?aiiice (D) i?ecaiaeeoue aei ia?aoai?aiiy
“iaaeoeaii(“ no?oeoo?e 2 a oaeo, yea aeyaey( na?ieia(/io aeoeai(noue
iiae(aio, a (ieiee i aeuo (oo. 7960, 8093, 5712, 766, 7956) ca
aeoeai(noue EIN oo. 8110. I?e/iio ni(aa(aeiioaiiy e(eueeino(
?icaaeoaeaieo ( e(i(eieo eaioetha(a I-IN, ui aoiaeyoue aei neeaaeo
iaaiiai EIN (a(aeiia(aeii, 25:75% aeey oo. 7960, 8093 oa 70:30% aeey
oo. 5712, 766, 7959), ia ia( nooo(aiai aieeao ia i?iya aeoeaiino(
aioeaaio.

3. A oie aea /an eneeicoaaiiy caeeoeo ?aiiice (D) a “aeoeai(e” no?oeoo?(
1 (oo. 8072 ) ia ni?e/eith( ci(i a aeoeaiino( EIN.

4. Ia a(aei(io a(ae eneeicoaaiiy, ?aiiiceethaaiiy aieiaiiai eaioethaa
“aeoeaii(“ no?oeoo?e 1 (oo. 7944, 8277) i?ecaiaeeoue aei cia/iiai
iaae(iiy na?ieia(/ii( aeoeaiino(.

?en. 4 “A?aeiinia” na?ieia?/ia aeoeai?noue EIN R.solanacearum

Iaea?aeai( aeai( aeicaieythoue c?iaeoe i?eiouaiiy, ui nooo(aei a i?iya(
na?ieia(/ii( aeoeaiino( aeine(aeaeoaaieo EIN iiaeeeai ( iniaeeaino(
aoaeiae (o I-IN, a naia o((( “aa?(aaaeueii(“ iaeano( eaioethaa, yea
neeaaea(oueny (c caeeoe(a N-aoeaoeeaetheicai(io (A) ( non(aei(o c iei
caeeoe(a L-?aiiice (B) ( (D). A caeaaeiino( a(ae ci(i o(i(/ii( aoaeiae
oe((( iaeano( aeoeaothoueny /e, iaaiaee, ae?aiothoueny aioeaaii(
aeaoa?i(iaioe EIN, ui iaoiiaeth( i?iya eiai na?ieia(/ii( aeoeaiino(
(oaae.3).

Oaaeeoey3

Oei no?oe

oo?e

“Aa?(aaaeueia” iaeanoue eaioethaa, ui iiaoi?th(oueny I-IN Aieea ia
na?ieia(/io aeoeai(noue EIN

1 (3)-(-L-Rhap-(1(3)- (-D-GlcpNAc-(1(2)-(-L-Rhap-(1(

D A
B aeoeaia

2 (3)-(-L-Rhap-(1(3)- (-D-GlcpNAc-(1(3)-(-L-Rhap-(1(

D A
B iaaeoeaia

3 (3)-(-L-Rhap-(1(3)- (-D-GlcpNAc-(1(3)-(-L-Rhap-(1(

(14 A
A

(-L-Xylp aeoeaia

5 (3)-(-L-Rhap-(1(3)- (-D-GlcpNAc-(1(2)-(-L-Rhap-(1(

(14 A
A

(-L-Xylp aeoeaia

6 (3)-(-L-Rhap-(1(3)- (-D-GlcpNAc-(1(2)-(-L-Rhap-(1(

D A
A(13

(-L-Rhap iaaeoeaia

E??i aeine?aeaeaiiy aioeaaiii? aeoeaiino? oe?ei? iieaeoee EIN, aoee
i?iaaaeai( aiaeiae/i? aenia?(iaioe c e?i?aeaie A. Ca aeeeth/aiiyi R.
solanacearum oo. 5712, niinoa?(aaeanue ei?aeyoe(y i(ae na?ieia(/iith
aeoeai(noth EIN ( e(i(aea A. I?aeoe/ii an( e(i(aee A i?iyaeyee cia/ii
a(eueoo na?ieia(/io aeoeai(noue, i(ae a(aeiia(aei( (i EIN.

Ine(eueee ia nueiaiaei( aeiaaaeaii, ui EIN aeayeeo aeae(a a?aiiaaaoeaieo
aaeoa?(e i?iyaeythoue aeineoue aenieee ?(aaiue (iioiiiiaeoethth/i?
aeoeaiino(, iaie aoei i?iaaaeaii aeine(aeaeaiiy (ioa?oa?iiiaaiii( oa
i?ioeiooeeiii( aeoeaiino( EIN R. solanacearum oa eiai ie?aieo
no?oeoo?ieo eiiiiiaio(a.

(ioa?oa?iiiaaiia aeoeai(noue

(ioa?oa?iiiaaiio aeoeai(noue EIN R. solanacearum aea/aee o ii?(aiyii(
aei oaei? eeane/iiai (ioa?oa?iiiaaia — EIN E. coli 055:A5 o(?ie“Sigma”.

Anoaiiaeaii, ui EIN R. solanacearum yaey?oueny ?iaeoeoi?ii ooai?aiiy
(-?ioa?oa?iio. (c iaea?aeaieo aeaieo (oaae. 4) aeo(ea?, ui
(ioa?oa?iiiaaiia aeoeai(noue i?aia?ao(a EIN R. solanacearum oa E. coli
055:A5 aoea aiaeia(/iith — 530 iae. Aeine(aeaeaiiy ie?aieo no?oeoo?ieo
eiiiiiaio(a iieaeoee EIN iieacaei, ui aieiaiee aianie a caaaeueio
aeoeai(noue iaeaaeeoue e(i(aeo A oa ei?-IA. Aecia/aii oaeiae iacia/io (o
ii?(aiyii( aei EIN) aeoeai(noue o?aeoe(( I-IN.

Oaaeeoey 4

(ioa?oa?iiiaaiia aeoeai(noue EIN oa eiai ie?aieo eiiiiiaio(a

Ooaie I?aia?aoe (ioa?oa?iiiaaiia aeoeai(noue, (I50/ie

R. solanacearum oo. 5712 EIN 530 + 110

I-IN 320 + 66

ei?-IA 1070 + 210

e(i(ae A 1070 + 210

E. coli O55:B5 (“Sigma”) EIN 530 + 110

I?ioeiooeeiia, aioeeaeeicia oa aioeiaoanoaoe/ia aeoeai(noue

I?e aea/aii( i?ioeiooeeiii? aeoeaiino( EIN R. solanacearum anoaiiaeaii,
ui EIN i(aeneethaaa ?(no ea?oeeiiie eaaai(a Eueth?n oa iaeaiiie A-16, ui
aeyaeeinue a i(aeaeuaii( ?o ?ici(?o. Oeae ?acoeueoao aoa
i(aeoaa?aeaeaiee a aenia?eiaio( c na?eiiith S-37.

I?e aecia/aii( aioeeaeeicii? aeoeaiino( EIN anoaiiaeaii, ui o?eaae(noue
aeeooy oaa?ei c aenia?eiaioaeueieie eaeeicaie i(ae eiai aieeaii
aeae(eueea ca(eueoo?oueny. I?e oeueiio EIN ia aieeaa( ia e(euee(noue
eaeeicieo ee(oei a anoeeoe/i(e ?(aeei( (oaae. 5) .

Oaaeeoey 5

Aieea EIN ia e(euee(noue eaeeicieo ee(oei oa i?iaeiaaeaiiy aeeooy ieoae
AeAA2 c e(ioioeeoa?iith eaeeai((th ?-388 ( e(ioi(aeiith eaeeai((th
L1210*

Aa?(aio

aeine(aeo E(euee(noue ee(oei a anoeeoe/i(e ?(aeei( (o107) O?eaae(noue
aeeooy ieoae, ae(a

E(ioi(aeia eaeeai(y L 1210

EIN 2.2 + 0.2 9.2 + 0.2

Eiio?ieue 2.5 + 0.2 7.6 + 0.2

E(ioioeeoa?ia eaeeai(y ?-388

EIN 4.2 + 0.2 8.0 + 0.2

Eiio?ieue 4.1 + 0.3 6.7 + 0.1

I?ei(oea: * ? ( 0,05

Anoaiiaeaii, ui EIN i?iyaeya ae?aaeaio aioeiaoanoaoe/io ae(th, yea
aeyaeyeanue o ciaioaii( ia’?i?a oa e(eueeino( iaoanoac(a (oaae. 6).
Iaiao(aeii a(aei(oeoe, ui aioeiaoanoaoe/iee aoaeo EIN ii?aeiothany c
eiai noeioeyoe(eiei aieeaii ia ?(no ia?aeiii? iooeeie.

I?e aecia/aii( aioeiaoanoaoe/ii( aeoeaiino( ie?aieo eiiiiiaio(a EIN
(oaae.6), anoaiiaeaii, ui EIN oa I-IN noaoenoe/ii aeinoia(?ii
ciaioothoue ia’?i oa e(euee(noue iaoanoac(a (ia 60-70% oa 40-50%,
a(aeiia(aeii). Aaaaeaiiy ieoai ei?-IA oaeiae cieaeo( ia’?i iaoanoac(a ia
63%. E(i(ae A ia i?iyaey( noaoenoe/ii aeinoia??ii? ae(? ia iaoanoaoe/iee
i?ioean.

Oaaeeoey 6

Aieea EIN ( eiai no?oeoo?ieo eiiiiiaio(a ia e(euee(noue ( ia’(i (ii3)
iaoanoac(a o ieoae c ea?oeeiiiith eaaai(a Eueth(n*

Aa?(aio aeine(aeo E(euee(noue iaoanoac(a Ia’(i iaoanoac(a

EIN 4.85 + 0.64 81.06 + 2.57

I-IN 5.45 + 0.51 63.27 + 27.21

Eiio?ieue 9.15 + 0.81 208.48 + 51.15

ei?-IA 5.40 + 0.63 52.35 + 12.50

e(i(ae A 7.05 + 0.70 90.41 + 22.68

Eiio?ieue (c AeINI) 6.75 + 0.70 138.83 + 30.46

I?ei(oea: * ? ( 0,05

Aaaeeeaei ia iaoo aeoieo ( aeyaeaiiy (ioiiiiaeoethth/eo aeanoeainoae
naia iie(naoa?eaeii( /anoeie iieaeoee EIN. Aaeaea iniiaiith i?iaeaiith
iiaeeeaiai aeei?enoaiiy a(aeiieo ia nueiaiaei( i?aia?ao(a
aaeoa?(aeueiiai iioiaeaeaiiy ( aenieee ?(aaiue (o i(?iaaiiino( oa
oiene/iino(, iaoiiaeaiee e(i(aeiith /anoeiith EIN.

?ICAe?E 6. OCAAAEUeIAIIss ?ACOEUeOAO?A AeINE?AeAEAIIss

A aeaiiio ?icae?e? aeeeaaeaii ocaaaeueiaiiy iniiaieo aenia?eiaioaeueieo
?acoeueoao?a aeine?aeaeaiiy, ye? aeicaieythoue c?iaeoe ianooii?
aeniiaee:

AENIIAEE

EIN an?o aeine?aeaeoaaieo ooai?a R.solanacearum ca nai?th no?oeoo?ith
iaeaaeaoue aei S-oi?ie – neeaaeathoueny ?c o?ueio eiiiiiaio?a:
I-niaoeeo?/iiai iie?naoa?eaeo, ie?ainaoa?eaeo ei?o oa e?i?aeo A.

Anoaiiaeaii 7 oei?a no?oeoo? I-IN, 6 c yeeo iienai( aia?oa. I-IN
aeine?aeaeoaaieo ooai?a R.solanacearum neeaaeathoueny (c o?e- , oao?a-
oa iaioanaoa?eaeieo eaioetha(a, ui iiaoi?ththoueny. Oa?aeoa?ieie aeey
I-IN oeueiai aeaeo aeyaeythoueny e(i(ei( oao?a- /e ?icaaeoaeai(
iaioanaoa?eaei? aoaeaaiaei? iine?aeiaiino?. ??cieoey i?ae ieie
noino?oueny ?ica(aeiinoae o

oei? eiio?ao?aoe?? ae(eiceaeiiai ca’yceo caeeoeo N-aoeaoeeaetheicai?io;

oei( eiai ca’yceo c non?aei(i caeeoeii ?aiiice;

iayaiino? a?/ieo cai?niee?a.

A?eueo?noue aeine?aeaeoaaieo ooai?a R.solanacearum oa?aeoa?eco?oueny
iayai?noth a neeaae? EIN a?eueoa ye iaeiiai oeio no?oeoo? I-IN.

Ia aeyaeaii caeaaeiino? i?ae aoaeiaith I-IN ? iaeaaei?noth ooaio aei
iaaiiai a?iaa?o, ?ineeie-oacy?ia, c yei? aoei aeae?eaii ooai, /e
aaia?ao(/ii( ciie, c yei( a?i iioiaeeoue.

Iniaeeaino? no?oeoo?e I-IN aieeaathoue ia i?iya na?ieia?/ii? aeoeaiino?
EIN. I?e oeueiio nooo?aa ?ieue iaeaaeeoue “aa??aaaeuei?e” iaeano?
eaioethaa, ui iiaoi?th?oueny, yea neeaaea?oueny ?c caeeoeo
N-aoeaoeeaetheicai?io ? aeaio non?aei?o c iei caeeoe?a ?aiiice. I?e
ci?iao a o?i?/i?e aoaeia? oe??? ciie aeoeaothoueny /e ae?aiothoueny
aioeaaii? aeaoa?i?iaioe iieaeoee EIN. Cia/io ?ieue o i?iya? na?ieia?/ii?
aeoeaiino? iieaeoeith EIN a?ae?a?a? oaeiae e?i?ae A.

sse(niee iiiinaoa?eaeiee neeaae ei?-IA EIN aeine(aeaeoaaieo ooai(a
aeyaey?oueny caaaeii oeiiaei aeey oaeiai a(eueoino( a?aiiaaaoeaieo
aaeoa?(e, iaeiae ca e(euee(niei ai(noii ie?aieo eiiiiiaio(a ?(ci( ooaie
cia/ii a(ae?(ciythoueny i(ae niaith.

E?i?aee A an?o aeine?aeaeoaaieo ooai?a R.solanacearum oa?aeoa?ecothoueny
iaeiioeiiith ea?oeiith ye?niiai neeaaeo. sse aeii?ioth/( eiiiiiaioe
e?i?aeo A oeueiai aeaeo aeyaeaii N14:0 (oao?aaeaeaiiaa), N16:0
(aaenaaeaeaiiaa) , 3-II N14:0 (3-a?ae?ieneoao?aaeaeaiiaa), 3-II N16:0
(3-a?ae?ieneaaenaaeaeaiiaa) oa 3-II N18:0 (3-a?ae?ieneieoaaeaeaiiaa)
eeneioe. Iayai(noue inoaiiuei( ( niaoeeo(/iith iciaeith i?aaenoaaiee(a
aeaeo R.solanacearum.

Anoaiiaeaii, ui EIN R. solanacearum yaey?oueny (iaeoeoi?ii ooai?aiiy
(-(ioa?oa?iio. A(aeiia(aeaeueieie ca (ioa?oa?iiiaaiio aeoeai(noue EIN (
e(i(ae A oa ei?-IA.

EIN R. solanacearum i?iyaey( aioeeaeeicio aeoeai(noue, iiaeiaaeoth/e
o?eaae(noue aeeooy aenia?eiaioaeueieo oaa?ei, a oaeiae ae?aaeaio
aioeiaoanoaoe/io ae(th, ciaiooth/e ia’(i oa e(euee(noue iaoanoac(a.

NIENIE ?IA(O, IIOAE(EIAAIEO CA OAIITH AeENA?OAOe((

Aa?aaiaoe E.Ae., I.A.Ei/a?iaa, TH.A.Eie?aeue,A.A.Io?an, I.A.Iineaeaiei,
I.N. A?iaa?neay Eeiiiieenaoa?eaeu Pseudomonas solanacearum e
Pseudomonas cichorii. // Iee?iaeie. aeo?i. — 1990.- 52, ?4. — n. 27-33.

Aa?aaiaoe E.Ae., ?uaaeei N.E., Aeyaethi N.O., A?iaa?neay I.N.,
Iineaeaiei I.A. Aeeeiiieeia?u Clavibacter michiganense e Pseudomonas
solanacearum — eiaeoeoi?u eioa?oa?iia. // Iee?iaeie. aeo?i. — 1990. —
52, ? 4. — n. 71-74.

Kocharova N. A., Knirel Yu. A., Varbanets L.D., Moskalenko N.V.,
Brovarskaya O.S., Muras V.A., Young J.M. Studies of O-specific
polysaccharide chains of Pseudomonas solanacearum lipopolysaccharides
consisting of structurally different repeating units. // Carbohydr. Res.
— 1993. — 250. — P.277-285.

Oaoeia A.N., Ei/a?iaa I.A., Eie?aeue TH.A., Aa?aaiaoe E.Ae., Iineaeaiei
I.A., Caeioeee A.A. Aioeaaiiua iieenaoa?eaeu aaeoa?ee. *38. No?oeoo?a
I-aioeaaiia Pseudomonas solanacearum ICMP 7942 e ICMP 78169. // Aeii?a.
oeiey. — 1993. — ? 11.-n. 1089 — 1094.

Aa?aaiaoe E.Ae., Iineaeaiei I.A., Caoa?iaa E.ss. No?oeoo?a
I-niaoeeoe/aneeo iieenaoa?eaeia Pseudomonas solanacearum,
i?aaenoaaeoaeae I aeiaa?a. // Iee?iaeie. aeo?i., — 1994.- 56, ? 1.-n.

Kocharova N. A., Shashkov A., Knirel Yu. A., Varbanets L.D., Moskalenko
N.V Structure of the O-specific polysaccharide of Pseudomonas
solanacearum strain 8089. // Carbohydr.Res. — 1994. — 259. — P.153-157.

Aa?aaiaoe E.Ae., Oiaeueei TH.I., I?eaeaoei I.A., AEoeiaa A.A.,
Iineaeaiei I.A. Aeieiae/aneay aeoeaiinoue eeiiiieenaoa?eaea Pseudomonas
solanacearum // Iee?iaeie. aeo?i.- 1995.- 57, ? 2.- n. 80 — 85.

Aa?aaiaoe E.Ae., Iineaeaiei I.A.. Eaaoi Y.I., Io?an A.A., AEeoeaae/ I.A.
Eco/aiea aioeaaiiie aeoeaiinoe eeiiiieenaoa?eaeia Burkholderia
solanacearum // Aeioeiey. — 1996.- 61, ?5 .- n. 835 — 841.

Aa?aaiaoe E.Ae., Ei/a?iaa I.A.,Eie?aeue TH.A., Iineaeaiei I.A. —
No?oeoo?a I-niaoeeoe/aneie iieenaoa?eaeiie oeaie eeiiiieenaoa?eaeia
Burkholderia (Pseudomonas) solanacearum ICMP 750, 8093, 8115, 7864, 7945
// Aeioeiey — 1996- 61, ? 5. — n. 807 — 813.

Iineaeaiei I.A. No?oeoo?ii-ooieoe(iiaeuei( aeine(aeaeaiiy
e(iiiie(naoa?eae(a Ralstonia solanacearum. — ?oeiien.

Aeena?oaoe(y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa a(ieia(/ieo iaoe
ca niaoe(aeuei(noth 03.00.07 — i(e?ia(ieia(y. — (inoeooo i(e?ia(ieia(( (
a(?onieia(( (i. Ae.E.Caaieioiiai IAI Oe?a(ie, i. Ee(a, 1999.

A ?iaio( i?iaaaeaii nenoaiaoe/ia aeine(aeaeaiiy e(iiiie(naoa?eae(a (EIN)
Ralstonia solanacearum. Aeae(eaii ( ioa?aeoa?eciaaii ca ye(niei (
e(euee(niei neeaaeii EIN ( (o ie?ai( no?oeoo?i( eiiiiiaioe
(I-niaoeeo(/i( iie(naoa?eaee, ie(ainaoa?eaee ei?o ( e(i(aee A) (c 17
ooai(a R. solanacearum, i?aaenoaaiee(a ?(cieo a(iaa?(a, ye(
a(ae?(ciyeenue oaeiae ?ineeiith-oacy(iii oa aaia?ao(/iei iioiaeaeaiiyi.
Anoaiiaeaii 7 oei(a no?oeoo? I-niaoeeo(/ieo iie(naoa?eae(a
aeine(aeaeoaaieo EIN, 6 c yeeo iienai( aia?oa. Iieacaii, ui iniaeeaino?
no?oeoo?e I-niaoeeo(/ieo iie(naoa?eae(a aieeaathoue ia i?iya
na?ieia?/ii? aeoeaiino? EIN: i?e ci?iao a o?i?/i?e aoaeia?
“aa?(aaaeueii(“ ciie aeoeaothoueny /e ae?aiothoueny aioeaaii?
aeaoa?i?iaioe iieaeoee EIN. Anoaiiaeaii, ui EIN R. solanacearum
(iaeoeo( ooai?aiiy (-(ioa?oa?iio, a(aeiia(aeaeueieie ca (ioa?oa?iiiaaiio
aeoeai(noue EIN ( e(i(ae A oa ei?-IA. EIN R. solanacearum i?iyaey(
ae?aaeaio aioeiaoanoaoe/io ae(th.

Eeth/ia( neiaa: Ralstonia solanacearum, e(iiiie(naoa?eae,
I-niaoeeo(/iee iie(naoa?eae, ie(ainaoa?eae ei?o, e(i(ae A,
(ioa?oa?iiiaaiia aeoeai(noue, aioeiaoanoaoe/ia ae(y..

Iineaeaiei I. A. No?oeoo?ii-ooieoeeiiaeueiua enneaaeiaaiey
eeiiiieenaoa?eaeia Ralstonia solanacearum. — ?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie nooiaie eaiaeeaeaoa aeieiae/aneeo iaoe
ii niaoeeaeueiinoe 03.00.07 — iee?iaeieiaey. — Einoeooo iee?iaeieiaee e
ae?onieiaee ei. Ae.E.Caaieioiiai IAI Oe?aeiu, a. Eeaa, 1999.

A ?aaioa i?iaaaeaii nenoaiaoe/aneia enneaaeiaaiea eeiiiieenaoa?eaeia
(EIN) Ralstonia solanacearum. Auaeaeaii e ioa?aeoa?eciaaii ii
ea/anoaaiiiio e eiee/anoaaiiiio ninoaao EIN e eo ioaeaeueiua no?oeoo?iua
eiiiiiaiou (I-niaoeeoe/aneea iieenaoa?eaeu, ieeainaoa?eaeu ei?a e
eeieaeu A) ec 17 ooaiiia R. solanacearum, i?aaenoaaeoaeae ?aciuo
aeiaa?ia, eioi?ua ioee/aeenue oaeaea ?anoaieai-oicyeiii e aaia?aoe/aneei
i?ienoiaeaeaieai. Onoaiiaeaii 7 oeiia no?oeoo? I-niaoeeoe/aneeo
iieenaoa?eaeia (I-IN) enneaaeoaiuo EIN, 6 ec eioi?uo iienaiu aia?aua.
I-IN 7 ooaiiia oa?aeoa?eciaaeenue eeiaeiie no?oeoo?ie n
oao?anaoa?eaeiuie iiaoi?ythueieny caaiueyie, ninoiyueie ec o?ao inoaoeia
L-?aiiicu e iaeiiai inoaoea N-aoeaoeeaetheicaieia. Iie ?acee/aeenue
iaaeaeo niaie aa?eaoeeyie a oeia eiioeao?aoeee aeeeiceaeie nayce inoaoea
N-aoeaoeeaetheicaieia : ( — a no?oeoo?ao 1 e 4 eee ( — a no?oeoo?a 2, a
oaeaea iiceoeeae aai i?ee?aieaiey e inoaoeo ?aiiicu : 1(2 a no?oeoo?a 1
eee 1(3 a no?oeoo?ao 2 e 4. Aeey aieueoeinoaa ooaiiia iaia?oaeaii
iaee/ea ?acaaoaeaiiuo iaioanaoa?eaeieo iiaoi?ythueony caaiueaa iniiao
eioi?uo ninoaaeyee eeiaeiua oao?anaoa?eaeu no?oeoo?u 1 eee 2. ?acee/ea
iaaeaeo ieie ninoiyei a oeia aieiaiai caianoeoaey: eneeica (no?oeoo?u 3
e 5) eee ?aiiica (no?oeoo?a 6) e ianoa aai i?eniaaeeiaiey e iniiaiie
oeaie. N?aaee enneaaeoaiuo ooaiiia cia/eoaeueii auaeaeyeny ii no?oeoo?a
I-IN oo. 8089: ii ninoiye ec eeiaeiuo o?enaoa?eaeiuo iiaoi?ythueony
caaiueaa, aeeth/athueo 2 inoaoea D-?aiiicu e 1 inoaoie
N-aoeaoeeaaeaeoicaieia (no?oeoo?a 7). Aeey iiiaeo ooaiiia auei
ii?aaeaeaii iaee/ea a ninoaaa I-IN ianeieueeeo oeiia no?oeoo?
iiaoi?ythuaainy caaia, i?e/ai eiiaaea ?acieoea iaaeaeo ieie auea
iaoneiaeaia eeoue niioiioaieai oeiia no?oeoo?. Ii ea/anoaaiiiio ninoaao
I-IN enneaaeoaiua ooaiiu iiaeii ?acaeaeeoue ia o?e oaiioeia:
i?aaenoaaeoaee I niaea?aeaee a ninoaaa I-IN L-?aiiico e
N-aoeaoeeaetheicaiei; II — e?iia oiai aua e eneeico; III — D-?aiiico e
N-aoeaoeeaaeaeoicaiei. Ei??aeyoeee iaaeaeo no?oeoo?ie I-IN e
i?eiaaeeaaeiinoueth ooaiia e ii?aaeaeaiiiio aeiaa?o eee
?anoaieth-oicyeio ia iaia?oaeaii. Iieacaii, /oi iniaaiiinoe no?oeoo?u
I-niaoeeoe/aneeo iieenaoa?eaeia aeeytho ia i?iyaeaiea na?ieiae/aneie
aeoeaiinoe EIN: i?e eciaiaieyo oeie/aneie no?oeoo?u “aa?eaaaeueiie“
ciiu (ninoiyuae ec inoaoea N-aoeaoeeaetheicaieia e aeaoo ninaaeieo n iei
inoaoeia L-?aiiicu) aeoeae?othony eee ye?aie?othony aioeaaiiua
aeaoa?ieiaiou iieaeoeu EIN. Onoaiiaeaii, /oi EIN R. solanacearum
iaeaaeaao eiioiiiiaeoee?othuae aeoeaiinoueth: eiaeooee?oao ia?aciaaiea
(-eioa?oa?iia (ioaaonoaaiiuie ca eioa?oa?iiiaaiioth aeoeaiinoue EIN
yaeythony eeieae A e ei?-IA), a oaeaea i?iyaeyao au?aaeaiiia
aioeiaoanoaoe/aneia aeaenoaea.

Eeth/aaua neiaa: Ralstonia solanacearum, eeiiiieenaoa?eae,
I-niaoeeoe-/aneee iieenaoa?eae, ieeainaoa?eae ei?a, eeieae A,
eioa?oa?iiiaaiiay aeoeaiinoue, aioeiaoanoaoe/aneia aeaenoaea.

Moskalenko N. V. Structure-function investigations of Ralstonia
solanacearum lipopolysaccharids. — Manuscript.

Thesis for a degree of candidate of biological sciences in speciality
03.00.07. — Microbiology. Zabolotny Institute of Microbiology, National
Academy of Sciences of Ukraine, Kyiv, 1999.

The dissertation is devoted to systematic investigations of Ralstonia
solanacearum lipopolysaccharides (LPS). LPS were isolated and
characterized from 17 strains of R. solanacearum, representatives of
various biovars which were differed by plant-host and geographical
origin. The seven types of O-specific polysaccharides structures were
determined, six of them were described for the first time. The
peculiarities of O-specific polysaccharides structure was show to
influence the LPS serological activity: the changes in chemical
structure of “variable” zone leads either to activation or screening the
antigenic determinants of LPS molecule.

R. solanacearum LPS was established to induce (-interferon production.
Lipid A and core-oligosaccharide were responsible for interferon
activity of LPS. R. solanacearum LPS showed an expressed antimetastatic
action.

Key words: Ralstonia solanacearum, lipopolysaccharide, O-specific
polysaccharide, core oligosaccharide, lipid A, interferoninducing
activity, antimetastatic action.

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