Чутливість ембріонів великої рогатої худоби різних стадій розвитку до дії низьких температур: Автореф. дис… канд. с.-г. наук / Мауссе Франсіско Сало

OE?A?INUeEA AEAAeAI?ss AA?A?IEO IAOE

?INOEOOO OAA?EIIEOeOAA OAAI

Iaonna O?ain?nei Naeiiii

OAeE 636.22/.28:57.08

*OOEEA?NOUe AIA??II?A AAEEEI? ?IAAOI? OOAeIAE ??CIEO NOAAe?? ?ICAEOEO
AeI Ae?? IECUeEEO OAIIA?AOO?

03.00.20 A?ioaoiieia?y

Aaoi?aoa?ao

aeena?oaoe?? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa n?euenueeiainiiaea?nueeeo iaoe

OA?E?A 1999

11

Aeena?oaoe??th ? ?oeiien.

?iaioa aeeiiaia o a?aeae?e? a?ioaoiieia?? a?aeoai?aiiy ? aaiii?
?iaeaia??? ?inoeoooo oaa?eiieoeoaa OAAI oa a eaai?aoi???
o?ainieaioaoe?? aia??ii?a AOCO “Na?oaiie” Iooe?nueeiai ?aeiio Noinueei?
iaeano?.

Iaoeiaee ea??aiee: aeieoi? n?euenueeiainiiaea?nueeeo iaoe

Aacoaeee Ieeiea Aeieo?iae/,

aee?aeoi? Oa?e?anueeiai a?ioaoiieia?/iiai

oeaio?o

Io?oe?ei? iiiiaioe :

-aeieoi? aaoa?eia?ieo iaoe, i?ioani?, Oa?ooa A?eai??e A?eai?iae/,
A?eioea?e?anueeiai aea?aeaaiiai aa?a?iiai oi?aa?neoaoo caa. eao.
aaoa?eia?iiai aeooa?noaa ? a?iaeieia??

-aeieoi? a?ieia?/ieo iaoe, noa?oee iaoeiaee ni?a?ia?oiee Aeoiaanueeee
Aaeaioei Eineiiae/ ?inoeoooo e??ia?ieia?? oa e??iiaaeeoeeie OAAI

I?ia?aeia onoaiiaa: Aea?aeaaiee iaoeiai-aeine?aeiee ?inoeooo
a?ioaoiieia??

? ooai?a i?e?ii?aai?ci?a, i. Ee?a

Caoeno aeena?oaoe?? a?aeaoaeaoueny ‘’29 ‘’ /a?aiy__ 1999 ?. i 12
aiaeei? ia can?aeaii? niaoe?ae?ciaaii? a/aii? ?aaee Ae27.821.01 i?e
A?eioea?e?anueeiio aea?aeaaiiio aa?a?iiio oi?aa?neoao?, 256400,
Ee?anueea iae., i. A?ea Oea?eaa, ie. Aie?, 8/1 ei?i. ? , aoae. ?

C aeena?oaoe??th iiaeia iciaeiieoenue o a?ae?ioaoe? A?eioea?e?anueeiai
aa?a?iiai aea?aeaaiiai oi?aa?neoaoo.

Aaoi?aoa?ao ?ic?neaiee ‘’ 25’’o?aaiy1999 ?.

A/aiee nae?aoa?

niaoe?ae?ciaaii? a/aii? ?aaee,

eaiaeeaeao a?ieia?/ieo iaoe
?iciooi?e I.?.

Caaaeueia oa?aeoa?enoeea ?iaioe

Aeooaeuei?noue oaie.

Aoaeoeai?noue canoinoaaiiy iaeiia?oeo iaoiae?a a?ioaoiieia??
a?aeoai?aiiy n.-a. oaa?ei, oaeeo ye eeiioaaiiy, oeia?ecaoe?y, aeyaeaiiy
noao? aia??ii?a oa ?i. iiaea c?inoaoe o?eueee i?e iayaiino? aaieo
aia??ii?a. ?aeeiei niiniaii caa?aaeaiiy aia??ii?a a cai?ioiueiio
aiaa?ic? ? ?o caii?iaeoaaiiy ? o?eaaea caa??aaiiy i?e iecueeeo
oaiia?aoo?ao.

Aeei?enoaiiy ?ic?iaeaieo niinia?a caii?iaeoaaiiy aia??ii?a ei??a ia
noaae?yo ?aii?o ii?oe ? aeanoioeeno i?ney aeeoieaiiy c ciie iaethoeeaea
iaio ?acoeueoaoeaia, oiaoi aea? iecueeee ??aaiue i?eaeeaeaiiy i?ney
ia?anaaeee ?aoeei??ioai. I/aaeaeii, ui aeey caii?iaeoaaiiy oaeeo
aia??ii?a iaiao?aeii aai iioei?coaaoe ie?ai? oaoiieia?/i? ia?aiao?e
?ai?oa ?ic?iaeaieo niinia?a caii?iaeoaaiiy, aai ?ic?iaeoe ?io?, a?eueo
iuaaeeea? niiniae ?o e??ieiina?aaoe?? aeey i?aeaeuaiiy ??aiy
caa?aaeaiino? ? i?eaeeaeaiiy ia?anaaeaeaieo aia??ii?a.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie.

Iiaeaia ?iaioa ia? ca’ycie c oaeeie iaoeiaeie i?ia?aiaie ye: noai?aiiy
iiaeo ?aaeei?a caii?iaeoaaiiy aia??ii?a nnaaoe?a c a?aooaaiiyi ?o
noaae?e ?icaeoeo, eeaneoeeaoe?y aia??ii?a ei?iae aeey ?o aeei?enoaiiy a
??cieo a?ioaoiieia?/iieo iaoiaeao a oaeiae iia’ycaia c i?ia?aiith
iaa/aiiy nooaeaio?a ii niaoe?aeueiinoyi a?ieia?/iiai i?io?eth.

Iaoa ? caaea/? aeine?aeaeaiue.

O ca’yceo c aeuaaeacaiei ? aeooaeueiei anoaiiaeoe i?e/eie ??cii?
/ooeeaino? aia??ii?a ei??a ??cieo noaae?e ?icaeoeo aei ae?? iecueeeo
oaiia?aoo? ? ?ic?iaeoe niin?a caii?iaeoaaiiy oaeeo ca?iaee?a. Aeey
aeinyaiaiiy iinoaaeaii? iaoe iaie ae??ooaaeeny ianooii? caaea/?:

1. Io?eiaoe aia??iie ei??a ??cieo noaae?e ?icaeoeo a?ae 8-ie ee?oeiii?
aei aeanoioeenoe i?ney aeeoieaiiy ?c ciie iaethoeeaea.

2. Aea/eoe no?ee?noue aei oaiia?aoo?iiai oieo aia??ii?a ??cieo noaae?e
?icaeoeo.

3. Aea/eoe ii?oieia?/i? a?aei?iiino? ? iniaeeaino? o?ainii?oo ?a/iaei
aeey aia??ii?a ??cieo noaae?e ?icaeoeo.

4. Ii??aiyoe caeaoi?noue aei aeaeeaaiiy aeaeiina?aiaaieo aia??ii?a ia
noaae?yo a?ae ?aiiuei? ii?oee aei aeeoieaii? aeanoioeenoe, ui
caii?iaeai? noaiaea?oiei iaoiaeii.

5. Canoinoaaoe iaoiaee e??ieiina?aaoe?? i?aai?a ? oeaiei nnaaoe?a aeey
caii?iaeoaaiiy aia??ii?a ia noaae?? aeeoieaii? aeanoioeenoe.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Cai?iiiiiaaia iiaa
eeaneo?eaoe?y aia??ii?a o a?aeiia?aeiino? c? noaae?yie ?o ?icaeoeo a?ae
8-ie ee?oeiii? aei aeanoioeenoe i?ney aeeoieaiiy ?c ciie iaethoeeaea;

iieacaii ui no?ee?noue aei oaiia?aoo?iiai oieo aia??ii?a ei??a ??cieo
noaae?e ?icaeoeo c?inoa? iinooiiai ii i??? ci?ie noaae?e ?icaeoeo.
Anoaiiaeaii, ui no?ee?noue c?inoa? c 0% aeey 8-ie ee?oeiieo aia??ii?a
aei 55% aeey ?aii?o ii?oe ? aeinyaa? 100% aeey eiiiaeoieo ii?oe
aeanoioeeno a cii? iaethoeeaea, aea iaaea? aei 63% aeey aeaioaeiaaieo
aeanoioeeno;

anoaiiaeaii, ui ia caa?aaeai?noue 8-ie ee?oeiieo aia??ii?a aieeaa?
o?eaae?noue aeniiceoe?? i?e 00 N;

iieacaii ??cieoeth i?io?eaiiy iniioe/ieo i?ioean?a o aia??ii?a ??cieo
noaae?e ?icaeoeo ;

cai?iiiiiaaii niin?a e??ieiina?aaoe?? aia??ii?a ia noaae?? i?ciuei?
aeanoioeenoe aac ciie iaethoeeaea, ui caaacia/o? ??aaiue i?eaeeaeaiiy a
2,4 ?ace aeuee ca ?acoeueoaoaie o?ainieaioaoe??, i?ae i?e aeei?enoaii?
noaiaea?oiiai iaoiaeo caii?iaeoaaiiy.

I?aeoe/ia cia/aiiy iaea?aeaieo ?acoeueoao?a.

Aeai?, io?eiai? a ?acoeueoao? ??ceiai ioieiaeaeaiiy aia??ii?a ei??a
??cieo noaae?e ?icaeoeo, c o?aooaaiiyi oa?aeoa?o i?io?eaiiy iniioe/ieo
i?ioean?a, iiaeooue aooe canoiniaai? aeey iioei?caoe?? ? noai?aiiy
?aaeei?a caii?iaeoaaiiy a?aeiia?aeiiai a?eo aia??ii?a; a a?ieia?/ieo ?
aaoa?eia?ieo aocao i?e neeaaeaii? i?ia?ai iaa/aiiy nooaeaio?a, a
iaoeiaeo ? ae?iaie/eo i?ia?aiao.

Iioei?ciaaiee iaie ?aaeei caii?iaeoaaiiy aia??ii?a aeei?enoiao?oueny i?e
caii?iaeoaaii? aeaioaeiaaieo aeanoioeeno a eaai?aoi??? o?ainieaioaoe??
aia??ii?a AOCO ‘’Na?oaiie’’ Iooe?nueeiai ?-io Noinueei? iaeano? ? a
Oa?e?anueeiio a?ioaoiieia?/iiio oeaio?? i?e aeeiiaii? iaoeiaeo ?
ae?iaie/eo i?ia?ai.

Iniaenoee aianie caeiaoaa/a.

A?eueo?noue aeine?aeaeaiue aeeiiai? aaoi?ii naiino?eii. Iaoiaee/i?
i?aeoiaee ? noaie aeine?ae?a a?aei?aoeueiaai? ni?eueii c iaoeiaei
ea??aieeii.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??.

Iniiai? iieiaeaiiy aeena?oaoe?? aoee i?aaenoaaeai? ia ?icaeyae a/aii?
?aaee Oa?e?anueeiai a?ioaoiieia?/iiai oeaio?o oa iaaiai?ai? ia
i?aeia?iaei?e iaoeiai-ae?iaie/?e eiioa?aioe?? “Oai??y oa i?aeoeea
ieai?iii? ni?aae o oaa?eiieoeoa?” (Oa?e?a, 1996).

Ianya oa no?oeoo?a aeena?oaoe??. Aeena?oaoe?y aeeeaaeaia ia 135
noi??ieao iaoeiiieniiai oaenoo ? neeaaea?oueny c anooio, iaeyaeo
e?oa?aoo?e, iaoa??ae?a oa iaoiae?a aeine?aeaeaiue, ?acoeueoaoe
aeine?aeaeaiue, aeniiae?a oa i?iiiceoe?? ae?iaieoeoao. I?noeoue 16
oaaeeoeue, 13 ?enoie?a oa 7 oioia?ao?e. Nienie e?oa?aoo?e aeeth/a? 206
iaeiaioaaiiue, o oiio /ene? 113 ?iicaiiith iiaith.

Ioae?eaoe??.

Aeena?oaioii iioae?eiaaii 5 iaoeiaeo noaoae a aeo?iaeao, ui aoiaeyoue a
ia?ae?e AAE Oe?a?ie, oaeeie ye ‘’Iaoeiai-oaoi?/iee atheaoaiue’’ ? 75
?inoeoooo oaa?eiieoeoaa OAAI; ‘’I?iaeaie cii?iaeaia??? oa aaoa?eia?ii?
iaaeeoeeie‘’ ? 4, oa?e?aaneiai ciiaaoa?eia?iiai ?inoeoooo, ‘’I?iaeai
A?ieia?? ? Iaaeeoeeie‘’ ? 8, iieoaanueei? iaaeeoeeinueei? aeaaeai??.
E??i oiai, iei aoa iioae?eiaaiee oacen aeey iaoeiai? eiioa?aioeoe??,
i?enay/aii? 80-??//th c aeiy ia?iaeaeaiiy /eai-ei?aniiiaeaioa AANAI?E
O.O.Aenia?a.

INIIAIEE CI?NO ?IAIOE

Iaoa??aee oa iaoiaee aeine?aeaeaiiy.

Ia’?eoii aeine?aeaeaiue aoee aia??iie ei??a a?ae 8-ie ee?oeiii?
noaae?? ?icaeoeo aei aeanoioeenoe i?ney aeeoieaiiy ?c ciie iaethoeeaea.
Aia??iie ??cieo noaae?e ?icaeoeo io?eioaaee oeyoii i?iieaaiiy
yeoeai?iaiae?a oa ?ia?a iaoee ei??a oa oaeeoeue-aeiii??a i?ney
iiia?aaeiuei? ia?iaee oaa?ei c iaoith io?eiaiiy ?aaeoe??
noia?iaoeyoe??.

Ioe?iea aeeoo?caeaoiino? aia??ii?a i?iaiaeeeany ii?oieia?/ii, ca
?acoeueoaoaie eoeueoeaoaaiiy in vitro ? oeyoii ia?anaaeee aia??ii?a
?aoeei??ioai.Caii?iaeoaaiiy aia??ii?a i?iaiaeeeiny noaiaea?oiei iaoiaeii
ca aeiiiiiaith oa?iiaeieo, ?ici?uaiiai o ai?eiaei? iinoaeeie Aeuetha?a.

I?eioeei ae?? oeueiai i?eno?ith caniiaaiee ia ianeaiiio ioieiaeaeaii?
(Inoaoei O.?., Aacoaeee I.Ae., 1989). Oaeaee?noue ioieiaeaeaiiy i?e
oeueiio aecia/aee ca aeiiiiiaith naiiieoo/iai iioaioe?iiao?a ENI-4.

Iiiaoaeaeea ioieiaeaeaiiy i?iaiaeeeiny oeyoii i?yiiai ia?aiino
aeine?aeieo a?iia’?eo?a c 370N a oaio/ee e?ae. Oaeaee?noue
ioieiaeaeaiiy a oeueiio aeiaaeeo aoea aecia/aia ca aeiiiiiaith
i?iaaaeaieo iaie iaoaiaoe/ieo ?ic?aooie?a.

?acoeueoaoe aeine?aeaeaiue

Io?eiaiiy aia??ii?a ??cieo noaae?e ?icaeoeo.

A aeine?aeao aoei aeei?enoaii 80 ei??a-aeiii??a, ye? iiceoeaii
i?i?aaaoaaee noia?iaoeyoe?th (817 aeiaoeo o?e), a?aeiia?aeii 10,21
aeiaoeo o?e ia eiaeiiai aeiii?a. ?c ?acoeueoao?a aeaeii, ui anueiai
aoei io?eiaii 665 a?ieia?/ieo ia’?eo?a, na?aae yeeo 168 (25%)
aeyaeeenue yeoeaee?oeiaie, a 497 (75%) — aia??iiaie. Oe? aeai?
na?ae/aoue i?i aenieo aoaeoeai?noue ia?iaee aeiii??a c iaoith io?eiaiiy
?aaeoe?? noia?iaoeyoe??. Aiae?c yeino? io?eiaieo aia??ii?a oa ?o
?iciiae?e ca noaae?yie ?icaeoeo iaaaaeai? a oaaeeoe? 2. Ca aeaieie
oe??? oaaeeoe? aeaeii, ui ?c 75% io?eiaieo aia??ii?a 46% aoee a?aei?iii?
yeino?, 27% — aeia?i?, 14% — caaeia?eueii?, a 13%- iacaaeia?eueii?. Oe?
?acoeueoaoe na?ae/aoue i?i aenieo ye?noue aaouee?anueeeo
?ai?iaeoeoeaieo ee?oei. I?enooi?noue yeoeaee?oei iiynith?oueny
aa??aaaeuei?noth oaii?a iaoeyoe??. sse aeaeii ?c ?acoeueoao?a, aia??iie
iaei??? e o??? ae noaae?? ?icaeoeo iiaeia aeeo/eoe ia i?ioyc?
aeae?eueeio aei?a ano?aeueiiai oeeeeo.

Oae, 8-ie ee?oeii? aia??iie aoee aeeo/ai? ia o?eueee ia i?ioyc? 4-ai
aeiy noaoaaiai oeeeeo, ui a?aeiia?aea? ?o o?c?ieia?/iiio a?eia?, aea ?
ia i’yoee aeaiue, eiee, cacae/ae, aia??iie ei?iae ciaoiaeyoueny ia
a?eueo i?iaeaeiooeo noaae?yo ?icaeoeo. ?acoeueoaoe io?eiaiiy aia??ii?a
iiaeaii a oaaeeoe? 1.

Oaaeeoey 1.

?acoeueoaoe aeeo/aiiy aia??ii?a ei??a ??cieo noaae?e ?icaeoeo.

?acoeueoaoe noia?iaoeyoe?? (ia 1
aeiii?a)AeaiueE?euee?noueE?euee?noueE?euee?noue
aeeo/aieoiieiaiaiaeiii??a,aeiaoeoyeoeaee?oei ? aia??ii?a, oo.
(%)oeeeeoaie.O?e, oo.yeoeaee?oeiaia??ii?aanueiai4*779
17(24)53(76)705*66614(25)43(75)5762322145(26)129(74)17471817436(26)100(7
4)13681012127(25)79(75)10691010523(26)66(74)89106516(18)27(82)33anueiai8
0817168(25)497(75)665- aeeo/aiiy i?iaiaeeeiny i?ney caaith
oaa?ei-aeiii??a

Aei oe??? noaae?? ne?ae a?aeiineoe an? ca?iaeee aei iiiaioo ?iieaioaoe??
(14-17-e aeaiue i?ney in?iai?iiy), aea a aeine?aeao ie aeei?enoiaoaaee
eeoa 9-10-aeaii? aean-oioeenoe, oi/a 3 c ieo aoee io?eiai? aaea ia 8-ee
aeaiue noaoaaiai oeeeeo.

A?aeiinii aeanoioeeno i?ney aeeoieaiiy oeae aeniiaie ia ae?eniee, oae ye
aei oe??? noaae?? ?icaeoeo ie a?aeiineee an? aeanoioeenoe, ui ao?aoeee
ciio iaethoeeaea, aac o?aooaaiiy e?eueeino? ee?oei a ieo ? ?o ?ici???a.

Oaaeeoey 2.

sse?noue aia??ii?a ??cieo noaae?e ?icaeoeo.

E?euee?noue aia??ii?a, oo. (%)Noaae?y anueiaio oiio
/ene??icaeoeoa?aei?i.aeia?acaaeia. iacaae.8-ie
ee?oeiiee5621(37,5)15(27)11(19,6)9(16,1)?aiiy
ii?oea9333(35,4)35(37,6)12(13)13(13,9)Eiiiaeoia
ii?oea10142(41,5)25(24,7)20(20)14(13,8)?aiiy
aeanoioeenoa4121(51,2)7(17,1)6(14,6)7(17,1)Aeanoioeenoa,ui
?icoe?th?oueny4331(72,2)3(6,9)9(20,9)-?icoe?aia
aeanoioeenoa8851(57,9)20(23)11(12,5)6(6,8)Aeanoioeenoa i?ney
aeeoiaiiy7329(39,5)27(37)-17(23,3)ANUeIAI497(75)228(46)132(27)69(14)66(1
3)Aaoa?iaaii?noue ?icaeoeo aia??ii?a a noaoaaeo oeyoao ei??a-aeiii??a
i?ney ai?iiiaeueii? ia?iaee aoea i?aeoaa?aeaeaia ua e oei, ui ia i?ioyc?
iaeiiai e oiai ae aeiy noaoaaiai oeeeeo aoee aeeo/ai? ca?iaeee ??ciiai
a?eo.

Ia iao iiaeyae, aia??iie ??cieo noaae?e ?icaeoeo, aea aeeo/ai? ?c
noaoaaeo oeyo?a aeiii?a ia i?ioyc? iaeiiai e oiai ae aeiy noaoaaiai
oeeeeo iathoue ??ciee a?e, ui iiynith?oueny ??ciiye?ni?noth naieo
aia??ii?a, ia??aiii??i?noth i?ioeano iaoeyoe??. Oe? ?acoeueoaoe
iaaaaeai? ia iae. 1.

*ooeea?noue aia??ii?a ei??a aei ioieiaeaeaiiy aei 00N.

Io?eiai? aia??iie iii?uaee a oa?iinoao i?e oaiia?aoo?? +370N, iio?i
ioieiaeaeoaaee c aecia/aiith oaeaee?noth aei o?eniaaii? oaiia?aoo?e,
aeo?eioaaee aecia/aiee /an ? iio?i i?iaiaeeee ei?ioei/ania (24 aiae.)
eoeueoeaoaaiiy in vitro.

Ia ii/aoeo aea/aiiy /ooeeaino? aia??ii?a ei??a ??cieo noaae?e ?icaeoeo
ie aecia/aee oaoiieia?/i? ia?aiao?e, ui iiaeooue aieeaaoe ia
caa?aaeai?noue aia??ii?a i?e ioieiaeaeaii?: e?ioeaao oaiia?aoo?o
ioieiaeaeaiiy, oaeaee?noue cieaeaiiy oaiia?aoo?e, aeniiceoe?th i?e
e?ioeaa?e oaiia?aoo?? oa iayai?noue e??ii?ioaeoi?a.

Ia ii/aoeo aea/aiiy /ooeeaino? aia??ii?a aei iecueeeo oaiia?aoo?
aia??iie (a?ae 8-ie ee?oeiieo aei aeanoioeeno i?ney aeeoieaiiy ?c ciie
iaethoeeaea) ioieiaeaeoaaee oeyoii i?yiiai ia?aiino ?c oa?iinoaoiaaii?
/aoee Iao?? i?e 370 N aei e?ioeaai? oaiia?aoo?e 00 N. I?e oeueiio
ioieiaeaeaiiy i?iaiaeeeiny c ??ciith oaeaee?noth ia iaioith 1600 0 N/n.
?acoeueoaoe iiaeai? a oaaeeoe? 3.

.
Oaaeeoey 3.

Caa?aaeai?noue aia??ii?a ei?iae i?e ioieiaeaeaii? aei 00N

E?euee?noueE?euee?noue aeeoo?caeaoieo aia??ii?a, oo./ %

Caa?a- Noaae?y ?icaeoeo aia??ii?a

oo. ca ii?oieia?/iith ioe?ieithca ?acoeueoaie eoeueoeaoaaiiy
aeai?noue

%
8-ieaeine?ae64(67)0(0)a0aee?oeii?eiio?ieue4-3(75)b?aiiyaeine?ae119(82)5(
46)b55bii?oeaeiio?ieue6-5(83)bEiiiaeoiaaeine?ae1211(92)9(75)b87c
ii?oeaeiio?ieue7-6(86)b ?aiiy
aeine?ae108(80)8(80)b87caeanoioeenoaeiio?ieue7-6(86)bAeano-oa,
uiaeine?ae1311(85)10(77)b100c?icoe?th?ouenyeiio?ieue8-6(75)b?icoe?aia.ae
ine?ae1210(83)10(83)b100caeanoioeenoaeiio?ieue9-7
(87)bAeanoioeenoaaeine?ae65(83)3(50)b63di?ney aeeoie.eiio?ieue54(80)b

Oeeo?e c iaeiaeiaei e?oa?iei ?iaeaenii icia/athoue, ui ?acoeueoaoe
aeinoia??i? (P>0,95), oeeo?e c ??ciei e?oa?iei ?iaeaenii icia/athoue, ui
?acoeueoaoe ia aeinoia??i?.

Aeey a?eueo oi/iiai aecia/aiiy oaiia?aoo?iiai ae?aiaciio, a yeiio
a?aeaoaa?oueny caaeaaeue aia??ii?a a?ae oaiia?aoo?iiai oieo, ie
ioieiaeaeoaaee aia??iie a?ae 8-ie ee?oei aei eiiiaeoii? ii?oee, c +370 N
aei +200 N (e?iiaoia oaiia?aoo?a). Io?eiai? ?acoeueoaoe iiaeai? a
oaaeeoe? 4.

?c aeaieo oaaeeoe? aeieeaa?, ui caa?aaeai?noue aia??ii?a an?o noaae?e
aeinoia??ii ia a?ae??ciyeanue iaeia a?ae iaeii? ? a?ae eiio?ieth. Oaeei
/eiii, e?eoe/ia oaiia?aoo?a ioieiaeaeaiiy, yea i?ecaiaeeoue aei
caaeaae? aia??ii?a, ciaoiaeeoueny a ae?aiacii? a?ae +20 aei 00 N.

I?e/eiith ??cieoe? a /ooeeaino? aia??ii?a ??cieo noaae?e ?icaeoeo aei
??ceiai ioieiaeaeaiiy iiaeooue aooe o?c?ieia?/i? ? a?io?i?/i?
a?aei?iiino? aia??ii?a ?, cie?aia, a?aei?iiino? a e?eueeino? oa yeino?
e?i?aeieo eiiiiiaio?a oeeoiieacie ? oeeoiieaciaoe/ieo iaia?ai
aeanoiia??a.

Aeae? ie aea/aee aieea oaeaeeino? cieaeaiiy oaiia?aoo?e ia
caa?aaeai?noue aia??ii?a.

Oaaeeoey 4.

Caa?aaeai?noue aia??ii?a ei?iae ??cieo noaae?e i?e ioieiaeaeaii? aei
200N

Noaae?y

E?euee?noueE?euee?noue aeeoo?caeaoieo

aia??ii?a, oo. /%Caa?aaeai?noue?icaeoeoaia??ii?a, oo.ca ii?oie

ioe?ieithca ?acoeueoaoaie eoeueoeaoaaiiy %8-ieaeine?ae55 (100)3 (60)80
aee?oeiieeeiio?ieue4-3 (75)?aiiyaeine?ae77 (100)5 (71)86
aii?oeaeiio?ieue6-5 (83)Eiiiaeoiaaeine?ae88 (100)7 (88)100
aii?oeaeiio?ieue7-6 (86)

Aeey aeine?aeaeaiiy oeueiai oaeoi?o aia??iie ia noaae?yo a?ae 8-ie
ee?oei aei eiiiaeoii? ii?oee ioieiaeaeoaaee a?ae 370 N aei 00 N c
??ciith oaeaee?noth (1, 10 ? 30 oa.), aeo?eioaaee ia i?ioyc? 1 oaeeeie
i?e 00 N, ia?aiineee a na?aaeiaeua c oaiia?aoo?ith +370N, ioe?ithaaee
ii?oieia?/ii ? eoeueoeaoaaee. ?acoeueoaoe aeaiiai aeine?aeo iiaeai? a
oaaeeoe? 5.

Oaaeeoey 5.

Caa?aaeai?noue aia??ii?a ei??a ??cieo noaae?e ioieiaeaeaieo aei 00N c
??ciith oaeaee?noth.

Noaae?y ?icaeoeoCaa?aaeai?noue i?e oaeaeeino? ioieiaeaeaiiy,
(%) aia??ii?a 10N/ oa 100N/oa960000/oa 8-ie aeine?ae
17 (1/6) 0 (0/4) 0 (0/5)ee?oeiia eiio?ieue 75 (4/4) 75 (3/4)
75 (3/4)?aiiy aeine?ae 57 (4/7) 50 (3/6) 45 (5/11) ii?oea
eiio?ieue 83 (5/6) 83 5/6 83 (5/6)Eiiiaeoia aeine?ae 83 (5/6)
67 (4/6) 75 (9/12)ii?oea eiio?ieue 86 (6/7) 86 (6/7) 85 (6/7)

?c iiaeaieo ?acoeueoao?a oaaeeoe? ?5 aeieeaa?, ui oaeaee?noue
ioieiaeaeaiiy aia??ii?a aei 00N ia /eieoue ?noioiiai aieeao ia
caa?aaeai?noue aia??ii?a a?ae 8-ie ee?oei aei eiiiaeoieo ii?oe.

Ianooiiei ia?aiao?ii, ui iiaea coiiaethaaoe /ooeea?noue aia??ii?a, ? ?o
aeniiceoe?y i?e oaiia?aoo?? 00 N. Aea/aiiy aieeao oeueiai oaeoi?o
i?iaiaeeeiny oeyoii ia?ai?uaiiy aia??ii?a c o?c?ieia?/ieo oiia o
na?aaeiaeua c oaiia?aoo?ith 00N ? aeniiceoe?yo o iueiio 7n., 1 oa 30
oaeeei, i?ney /iai aia??iie ioe?ithaaee ii?oieia?/ii oa eoeueoeaoaaee.
?acoeueoaoe iiaeai? a oaaeeoe? 6.

C oaaeeoe? aeaeii, ui aeey aia??ii?a ia noaae?? eiiiaeoii? ii?oee ?
?icoe?aii? aeanoioeenoe ??aaiue caa?aaeaiino? ia caeaaeeoue a?ae
aeniiceoe??. Oei /anii, 8-ie ee?oeii? aia??iie aeo?eiothoue eeoa
ei?ioei/ani? aeniiceoe?? a 33% aeiaaeeao. Oeae oaeo aeicaiey?
i?eionoeoe, ui iioeiaeaeoth/o ae?th iecueeeo oaiia?aoo? ia 8-ie ee?oeii?
aia??iie /anoeiai coiiaeth? o?eaae?noue aeniiceoe??.

Oaaeeoey 6.

E?euee?noue aeeoo?caeaoieo aia??ii?a i?ney ioieiaeaeaiiy aei 0iN i?e

??cieo aeniiceoe?yo.

Noaae?y ?icaeoeoCaa?aaeai?noue aia??ii?a (%), i?e
yeniiceoe??:aia??ii?aiaioa 7 n 1 oa30 oa8-ieaeine?ae33 (2/6)0 (0/6)0
(0/5)ee?oeieiio?ieue75 (3/4)75 (3/4)75 (3/4)Eiiiaeoiaaeine?ae75 (6/8)75
(9/12)60 (6/10)ii?oeaeiio?ieue87 (6/7)87 (6/7)86
(6/7)?icoe?aiaaeine?ae80 (8/10)83 (10/12)67 (6/9)aeanoioeenoaeiio?ieue78
(7/9)78 (7/9)78 (7/9)Iiaeeeai, aecia/iith oiiaith a oeueiio aeiaaeeo ?
iayai?noue oaciaeo ia?aoiae?a neeaaeiaeo eiiiiiaio?a oeeoiieaciaoe/ieo
iaia?ai ?c ??aeeie?enoae?/iiai a oaa?aeie?enoae?/iee noai. Iaie aoea
aea/aia caoenia ae?y ae?oea?eio i?e ??ceiio ioieiaeaeaii? aia??ii?a aei
00 N. Aia??iie ia noaae?yo a?ae eiiiaeoii? ii?oee aei aeanoioeenoe i?ney
aeeoieaiiy ?c ciie iaethoeeaea iane/oaaee ae?oea?eiii a eiioeaio?aoe??
1,2 I i?e +370 N, iio?i ia?aiineee a na?aaeiaeua c oaiia?aoo?ith
oaio/iai eueiaeo, aeo?eioaaee ia i?ioyc? iaei??? oaeeeie ? iiaa?oaee a
o?c?ieia?/i? oiiae. I?ney aeaaaeaiiy ae?oea?eio 0,5 I ?ic/eiii naoa?ice
aia??iie a?aeieaaee ? ioe?ithaaee ye?noue ye ? a iiia?aaei?o aeine?aeao.
Eiio?ieueio a?oio aia??ii?a i?aeaeaaaee aiaeia?/iiio iane/aiith, iaeiae
ioieiaeaeaiiy ?o ia i?iaiaeeee (Oaaeeoey 7).

Oaaeeoey 7.

Caa?aaeai?noue aia??ii?a ei??a ??cieo noaae?e iane/aieo 1,2 I ?ic/eiii

ae?oea?eio, i?e ioieiaeaeaii? aei 00N

E?euee?noueE?euee?noue aeeoo?caeao-

ieo aia??ii?a, oo., % Caa?a-

Noaae?y ?icaeoeoaia??ii?a ca ii?oieia. Ioe?ieith ca ?acoeueoaie
eoeueoeaoaaiiyaeai?noue, %Eiiiaeoia aeine?ae109 (90)7
(70)99ii?oeaeiio?ieue76 (86)5 (71)?icoe?aiaaeine?ae96(100)7
(78)94aeanoioeenoaeiio?ieue66(100)5 (83)Aeanoioeenoaaeine?ae66 (100)4
(67)83i?ney aeeoie.Eiio?ieue55 (100)4 (80)

Aeai? oaaeeoe? 7 iieacothoue, ui i?e ioieiaeaeaii? aia??ii?a, ye?
aea/aeeny, ae?oea?ei ia ci?ith? ?o caa?aaeai?noue ia noaae?yo a?ae
eiiiaeoii? ii?oee aei noaae?? aeanoioeenoe i?ney aeeoieaiiy ?c ciie
iaethoeeaea.

Caii?iaeoaaiiy aia??ii?a ??cieo noaae?e ?icaeoeo.

Aenia?eiaioe ii iecueeioaiia?aoo?i?e eiina?aaoe?? ie ii/aee aeine?aeii
ii caii?iaeoaaiith aia??ii?a an?o noaae?e ?icaeoeo, ui aea/aeeny iaie,
aeei?enoiaoth/e noaiaea?oi? iaoiaee. sse e??ii?ioaeoi? aeei?enoiaoaaee
ae?oea?ei o eiioeaio?aoe?? 1,2 I. Iane/aiiy ? aeaeaeaiiy e??ii?ioaeoi?o
i?iaiaeeeiny iaeiinooi?i/aoi. Caii?iaeoaaiiy i?iaiaeeeinue ?c
aeei?enoaiiyi oa?iiaeieo, iii?uaiiai a ai?eiaeio noaeeie Aetha?a. I?e
oeueiio ?aaeei caii?iaeoaaiiy a?aeiia?aeaa caeiio ianeaiiai
ioieiaeaeaiiy aei e?ioeaai? oaiia?aoo?e -30 2,40N.

I?ney aeaeaeaiiy e??ii?ioaeoi?o aia??iie ioe?ithaaee ii?oieia?/ii ?
iii?uaee ia ei?ioei/ania eoeueoeaoaaiiy in vitro.

O? aia??iie, ui caa?aaee aeeoo?caeaoi?noue i?ney
caii?iaeoaaiiy-a?aeoaaaiiy, ia?anaaeaeoaaeenue ei?iaai ?
oaeeoeyi-?aoeei??ioai iao??o?a?/iei iaoiaeii. ?acoeueoaoe aeine?aeo
iiaeai? a oaaeeoe? 8.

Oaaeeoey 8.

Caa?aaeai?noue aia??ii?a ei??a ??cieo noaae?e i?ney e??ieiina?aaoe??.

Noaae?y

E?euee?noueE?euee?noue aeeoo?caeaoieo

aia??ii?a, oo./%?icaeoeoaia??ii?a,

oo. ca ii?oie. ioe?ieaieca ?acoeueoaoaie eoeueoeaoaaiiy?aiiy ii?oea10
7 (70)a3 (30)cEiiiaeoia ii?oea1210 (83)a9 (75) d?aiiy aeanoioeenoa1110
(91)a8 (73) d?icoe?aia aeanoioeenoa1311 (85)a10 (77) dAeanoioeenoa,ui

?icoe?th?oueny109 (90)a8 (80) dAean-oa i?ney

aeeoieaiiy73 (43)b-*

*- aia??iie ia eoeueoeaoaaee, a a?ae?aco ia?anaaeeee ?aoeei??ioai.

Aiie i?aeoaa?aeaeothoue, ui noaiaea?oiee iaoiae caii?iaeoaaiiy ?
aoaeoeaiei i?e caii?iaeoaaii? aia??ii?a ia noaae?yo a?ae eiiiaeoii?
ii?oee aei ?icoe?aii? aeanoioeenoe. Aeey oni?oiiai caii?iaeoaaiiy ?aii?o
ii?oe ? aeae? i?iaeaeiooeo noaae?e ?icaeoeo aia??ii?a iaiao?aeii
oaeineiiaethaaoe oaoiieia?th e??ieiina?aaoe??. A iaei?e ?c ni?ia
oeueiai ie i?iaiaeeee aeine?aee ii caii?iaeoaaiith ?aii?o ii?oe ? i?ci?o
aeanoioeeno ei?iae c ??ciith oaeaee?noth c aeei?enoaiiyi a yeino?
e??ii?ioaeoi?o ae?oea?eio a 1,2I eiioeaio?aoe??. ??cia oaeaee?noue
ioieiaeaeaiiy aeinyaaeany oeyoii a?aeiia?aeiiai ?aaoethaaiiy i?eno?ith
aeey caii?iaeoaaiiy. AEeoo?caeaoi?noue ?icii?iaeaieo aia??ii?a
aecia/aeany ii?oieia?/ii oa ca ?acoeueoaoaie ei?ioei/aniiai
eoeueoeaoaaiiy. Io?eiai? ?acoeueoaoe iaaaaeai? a oaaeeoe? 9.

?acoeueoaoe oeueiai aeine?aeo na?ae/aoue i?i oa, ui oaeaee?noue
ioieiaeaeaiiy ia cae?enith? aieeao ia caa?aaeai?noue ?aii?o ii?oe.
Ioaea, aeey ?o e??ieiina?aaoe?? ne?ae ooeaoe ?io? oaoiieia?/i?
i?aeoiaee. Aeey i?ci?o aeanoioeeno niinoa??aa?oueny /?oea oaiaeaioe?y
i?aeaeuaiiy caa?aaeaiino? i?e cieaeaii? oaeaeeino? ioieiaeaeaiiy. Oae,
cieaeaiiy oaeaeeino? caii?iaeoaaiiy aei 0,150N/oa ni?eyei i?aeaeuaiith
caa?aaeaiino? aia??ii?a a 2,4 ?ace a ii??aiyii? c noaiaea?oiei ?aaeeiii
caii?aaeoaaiiy.

Oaaeeoey 9.

Caa?aaeai?noue aia??ii?a ei??a i?e caii?iaeoaaii? c ??ciith oaeaee?noth.

Noaae?yE?euee?noue aia??ii?a aeeoo?caeaoieo / caii?iaeaieo, oo,
ioieiaeaeaieo i?e oaeaeeino?: iN / oa?icaeoeo0,60,30,15?aiiy ii?oea2/9
(22)a 3/10 (30) a2/8 (25) aAeano-oa i?ney aeeoie.0/5 (0) b1/7 (14) b2/6
(33) d a aeoaeeao aeacaia caa?aaeai?noue aia??ii?a, %.

Aeey aea/aiiy aieeao e??ii?ioaeoi?a ia caa?aaeai?noue oeeo aia??ii?a, ie
aeei?enoaee ae?oea?ei a eiioeaio?aoe?yo 1,2 I , 1,8 I ? 2,4 I. Iane/aiiy
e??ii?ioaeoi?ii o an?o o?ueio aeiaaeeao i?iaiaeeee a iaeei aoai.
Aeaaaeaiiy ae?oea?eio aeey ia?oeo aeaio aeiaaee?a i?iaiaeeee a iaeei
aoai o 0,5 I ?ic/ei? naoa?ice, a aeey inoaiiueiai aeiaaeeo — a aeaa
aoaia o 0,5 ? 1,0 I ?ic/eiao naoa?ice. Caii?iaeoaaiiy i?iaiaeeee c
na?aaeiueith oaeaee?noth 0,15 0 N / oa. Io?eiai? ?acoeueoaoe iaaaaeai? a
oaaeeoe? 10.

Oaaeeoey 10.

Aieea eiioeaio?aoe?? ae?oea?eio ia caa?aaeai?noue aeanoioeeno i?ney
aeeoieaiiy i?e e??ieiina?aaoe??.

E?euee?noue aia??ii?a, oo.E?euee?noue o?eueieoEiioeaio?a-

oe?y ae?oea?eio, Icaii?i-

aeaieoaeeoo?caeao-

ieo i?ney ?ic-iy*Caa?aaea-

i?noue, %?aoeei??o?a, aie. (i?eaeea-oue, %)1,27343a0 b1,87457 a1 (25)
c2,48450 a2 (50) d — an? aia??iie aoee o?ainieaioiaai? ?aoeei??ioai

?c aeaieo oaaeeoe? 10 aeaeii, ui ca?eueoaiiy eiioeaio?aoe??
e??ii?ioaeoi?a aeinoia??ii i?aeaeuo? caa?aaeai?noue aeaioaeiaaieo
aeanoioeeno. Ca?aene aeieeaa? aeniiaie, ui i?e aeei?enoaii?
noaiaea?oiiai niiniao caii?iaeoaaiiy aeey i?aeaeuaiiy caa?aaeaiino? 9-10
— aeaiieo aia??ii?a ei??a iio??aii aeei?enoiaoaaoe ye i?aeaeuaiiy
eiioeaio?aoe?? ae?oea?eio, oae ? iia?euei? oaeaeeino? cieaeaiiy
oaiia?aoo?e.

Iniaeeaino? o?ainii?oo aiaee ? ?ic/eiaieo ?a/iaei a aia??iie ??cieo
noaae?e ?icaeoeo.

A?eueo?noue e??ii?ioaeoi??a, ui caaacia/othoue caa?aaeai?noue ee?oeiieo
no?oeoo? i?e cieaeaii? oaiia?aoo?e, ? i?iieeath/eie ?a/iaeiaie ? aeey
aoaeoeaiiai caoenoo aia??ii?a aiie iiaeii? aooe i?enooi?ie a na?aaeei?
ee?oei. Iai?aaeeueia aeei?enoaiiy e??ii?ioaeoi??a iiaea i?ecaanoe aei
caaeaae? aia??ii?a ua aei caii?iaeoaaiiy a ?acoeueoao? iniioe/iiai
e?ceno.

O ca’yceo c oeei, i?iaeaia o?ainii?oo aiaee ? i?iieeath/eo ?a/iaei /a?ac
oeeoiieaciaoe/i? iaia?aie aia??ii?a yaeythoue niaith ie?aio ? aaeueie
aaaeeeao caaea/o aeey iiyniaiiy a?aei?iiino? a /ooeeaino? aei
caii?iaeoaaiiy aia??ii?a ??cieo noaae?e ?icaeoeo.

C iaeyaeo iai?iieo i?ioean?a aeanoiia?e 8-ie ee?oeiieo aia??ii?a ei??a
ciaoiaeyoueny o ??aiioe?iiiio noaiiaeu?, oiaoi any iiaa?oiy
oeeoiieaciaoe/ii? iaia?aie aeanoiia?a aa?a o/anoue a iai?i? ?a/iaei .

Aeey aaaaoiee?oeiieo no?oeoo? ea?oeia ci?ith?oueny, oae ye a o?ainii?o?
ia caae?yi? i?aeee?oeii? eiioaeoe, a a ee?oeie aioo??oi?o oa??a oaeeo
no?oeoo? ?a/iaeie iiaeooue i?iieeaoe o?eueee /a?ac ciai?oi? oa?e. Oaea
ea?oeia oa?aeoa?ia aeey aia??ii?a nnaaoe?a, oae ye a ia??iae naiai
?icaeoeo aiie caciathoue cia/ieo no?oeoo?ieo ci?i: a?ae iaeiiee?oeiii?
ceaioe aei aaaaoiee?oeiieo ? aaaaoioa?iaeo aeaioaeiaaieo aeanoioeeno.

Aia??iie aei 8-ie ee?oeiii? noaae?? ?icaeoeo i?aaenoaaeythoue niaith
noeoii?noue aeanoiia??a ? an? aiie ciaoiaeyoueny a iaeiaeiaiio
noaiiaeu?. Noaiaoe/ii oaea no?oeoo?a iaaaaeaia ia iae.2.

Aeaia noaae?y ?icaeoeo aia??ii?a ? iaeni?eyoeea?oith aeey ??aiioe?iiiai
o?ainii?oo ?a/iaei aei an?o aeanoiia??a, ?, i/aaeaeii, ne?ae aoei a
i/?eoaaoe iaee?aueo ?acoeueoao?a i?e e??ieiina?aaoe?? oaeeo aia??ii?a.
Iaeiae, ye iieacothoue iao? aeine?aeaeaiiy ? aeine?aeaeaiiy ?ioeo
aaoi??a (Trouson A.O., Willadsen S.M., 1976), caa?aaeai?noue aia??ii?a
?aii?o noaae?e aeaeaei ia iaee?aua. Ia iao iiaeyae oea iiynith?oueny
ianooiiei.

Caaaeath/e ia aeinoaoiuei ??aee?nia aeei?enoaiiy aeey i?aeoe/iiai
a?aeoai?aiiy noaaea ?aii?o aia??ii?a ei?iae, ?o caii?iaeothoue c
aeei?enoaiiyi ?aaeei?a, ?ic?iaeaieo aeey i?ci?o ii?oe ? ?aii?o
aeanoioeeno, ye?, a naith /a?ao, oe?iei ae

ei?enoiaothoueny c aeaiith iaoith.

?aii? noaae?? aia??ii?a ?noioii a?ae??ciythoueny a?ae a?eueo
i?iaeaeiooeo noaae?e ?icaeoeo ii i?iieeiino? iaia?ai . Ia noaae??
?aiiuei? ii?oee aia??iie iaaoaathoue aeaioa?iai? no?oeoo?e, noaiaoe/ia
cia?aaeaiiy yei? iaaaaeaia ia iae.3.

Oiiae o?ainii?oo aiaee ? ae?oea?eio aeey ee?oei ciai?oiueiai ?
aioo??oiueiai oa??a ?noioii a?ae??ciythoueny. Uiaei e??ieiina?aaoe?? oea
icia/a?, ui yeui ?aaeei cieaeaiiy oaiia?aoo?e ? niin?a canoinoaaiiy
e??ii?ioaeoi?a aoaea i???ioiaaiee ia aeeaiei?ici?uai? ee?oeie, oni?o i?e
caii?iaeoaaii? aoaea aeinyaiooee.

Ia a?eueo i?ci?o noaae?yo ?icaeoeo i?io?eaiiy iai?iieo i?ioean?a a
aia??iiao ua a?eueo oneeaaeith?oueny. Iayai?noue aeae?eueeio
aioo??oi?o oa??a ? aeanoioeae? ?iaeoue oaeee aia??ii ua a?eueo
??cii??aeiei ?, i/aaeaeii, ui ee?oeie aioo??oi?o oa??a i?eeiathoue
o/anoue o o?ainii?o? ?a/iaei cia/ii iaioa.

Noaiaoe/ii iai?ii? i?ioeane i?io?eathoue ianooiiei /eiii:

1. Iaaeieeoi? na?aaeiaeua ciai?oi? ee?oeie aioo??oi? aeanoiia?e.

2. Iaaeieeoi? na?aaeiaeua o?ioiaeano aeanoioeaeue.

3. Aeanoioeaeue aioo??oi? aeanoiia?e aeanoioeaeth.

Noaia aoaeiae aia??iia oaei? noaae?? ?icaeoeo iaaaaeaia ia iae.4.

Uiaei e??ieiina?aaoe?? oea icia/a?, ui i?e noaiaea?oiiio ?aaeei?
caii?iaeoaaiiy oe? ee?oeie iiaeooue ia anoeaiooe iicaooeny
aioo??ee?oeiii? aiaee aei ianoaiiy oaiia?aoo?e aioo??ee?oeiii?
e?enoae?caoe??, ui i?ecaiaeeoue aei e?ceno a i?ioean? ?icii?iaeoaaiiy.

Aiae?c o?ainii?oo ?a/iaei o aia??iiao aoa aeei?enoaiee iaie aeey
ooi/iaiiy ?o eeaneo?eaoe?? ca noaae?yie ?icaeoeo.

Eeaneo?eaoe?y ia?aae?iieaioaoe?eieo aia??ii?a ei??a.

sse i?aaeei, eiaeia a?oia aeine?aeiee?a aeei?enoiao? aeanio ooi/iaio
eeaneo?eaoe?th. Iao? aeine?aeaeaiiy i?iaiaeeeeny ia aia??iiao ei??a,
ii/eiath/e c? noaae?? 8 ee?oei aei noaae?? aeeoieaiiy ?c ciie
iaethoeeaea. Oe? aia??iie aoee ?icaeo? ia 7 a?oi.

A oaaeeoe? 11 i?eaaaeaia eeaneo?eaoe?y aia??ii?a o a?aeiia?aeiino? c?
noaae??th ?icaeoeo, ?o a?eii o aiaeeiao i?ney caie?aeiaiiy, e?euee?noth
ee?oei ? noaiith aoaeiae. ?c cai?iiiiiaaii? iaie noaie eeaneo?eaoe??,
?aii? aia??iie aei noaae?? 8-ie aeanoiia??a (?yaeie 1 oaaeeoey 11)
aeioe?eueii eeaneo?eoaaoe ca caaaeueiith e?euee?noth ee?oei (1-ee?oeiia
— ceaioa; 2-o ee?oeiia; 4-o ? 8-ie ee?oeii? ).

C iaeyaeo iniioe/ieo i?ioean?a noaae?th ii?oee aeioe?eueii ?icaeoe ia
aea? noaae?? : ?aiii? ii?oee (16 ee?oei) ? eiiiaeoii? ii?oee (32
ee?oeie).

O ca’yceo c aneio?iii?noth iiae?eo ee?oei e?euee?noue aeanoiia??a o
ca?iaeeo ie aeaco?ii i?eaeecii, aeiaea?aeoth/enue oi/ee ci?o, ui ia
noaae?yo eiiiaeoii? ii?oee ? ?aiiuei? aeanoioeenoe iiae?e aeanoiia??a
i?iaeiaaeo? iineoe neio?iiiee oa?aeoa? (?yaeee 3 ? 4 noiaioey 3,
oaaeeoey 11), uiaei a?eueo i?iaeaeiooeo noaae?e, iiae?e iiai?noth
iineoue aneio?iiiee oa?aeoa? (?yaeee 5, 6 ? 7 noiaioey 3, oaaeeoey 11.).

Noaae?th aeanoioeenoe, ui oa?aeoa?eco?oueny aeieeiaiiyi aioo??oiuei?
ii?iaeieie — aeanoioeaeth, a?ae iiiaioo aeieeiaiiy inoaiiuei? aei
iiiaioo aeeoieaiiy ?c ciie iaethoeeaea, ie ?icaeee ia 4 noaae??.

Ia?o? o?e c ieo oa?aeoa?ecothoueny ci?iith naiiai aeanoioeaeth: ?aiiy
aeanoioeenoa, aeanoioeenoa, ui ?icoe?th?oueny ? ?icoe?aia aeanoioeenoa.
*aoaa?oa ca?iaeie, ui aeeoieany c ciie iaethoeeaea, oa?aeoa?eco?oueny
iayai?noth aaeeeiai ?ic?ineiai o?ioiaeanoo, ui neeaaea?oueny c e?oiieo
ee?oei, ? eiiiaeoiiai aia??iaeanoo, ?c yeiai aoaea ?icaeaaoeny a
iiaeaeueoiio ie?ae, ? ui neeaaea?oueny c ae??aieo, u?eueii oiaeiaaieo
ee?oei.

Cai?iiiiiaaia iaie eeaneo?eaoe?y aeanoioeeno, ui ciaoiaeeoueny a cii?
iaethoeeaea, aeaui a?ae??ciy?oueny a?ae cai?iiiiiaaii? ?ioeie

aaoi?aie (Schneider H.J.Jr., Castleberry R.S. Griffin J.L. 1980, Oeoia
?..ss. ? Na?aa?a ?. ?. 1981 ), ye? aeae?eythoue o?eueee noaae?th
?aiiuei? ? i?ciuei? aeanoioeeno.

Aeniiaee

1. Niinoa??aa?oueny aeniea a?eiaa aaoa?iaaii?noue iiioeyoe?? aia??ii?a,
io?eiaieo ?c noaoaaeo oeyo?a ei??a, ye? noia?iaoethaaee ia i?ioyc?
iaeiiai ? oiai ae aeiy noaoaaiai oeeeeo, ui iiynith?oueny
ia??aiii??i?noth i?ioeano iaoeyoe?? ? ??cieoeath a /an? caie?aeiaiiy
yeoeaee?oei.

2. Caie?aeithaai?noue yeoeaee?oei ia caeaaeeoue a?ae aeiy noaoaaiai
oeeeeo aeiii?a ? ciaoiaeeoueny a iaaeao 74-82% , a aeo?ae caaaeueii?
e?eueeino? aia??ii?a aeinoia??ii ia caeaaeeoue a?ae noaae?? ?icaeoeo,
ii/eiath/e c 8-ie ee?oeiii? noaae??, ? eieeaa?oueny a iaaeao 65-89%.

3. *ooeea?noue aia??ii?a ei??a aei ??ceiai ioieiaeaeaiiy a?ae 370 N aei
00 N caeaaeeoue a?ae noaae?e ?o ?icaeoeo: 8-ie ee?oeii? aia??iie ia
ia?aiinyoue oaeaeeiai ioieiaeaeaiiy. ?aii? ii?oee aeoaea /ooeea?, oiio
ui aeia a?eueoa iieiaeia ca?iaee?a. I?aeaeuaiith /ooeea?noth
oa?aeoa?ecothoueny ? aeaioaeiaai? aeanoioeenoe. No?ee?noue eiiiaeoieo
ii?oe ? aeanoioeeno a cii? iaethoeeaea aeniea ? aeinoia??ii ia
a?ae??ciy?oueny a?ae eiio?ieth.

4. Oaiia?aoo?a, i?e ye?e a?aeaoaa?oueny caaeaaeue aia??ii?a ei??a i?e
??ceiio ioieiaeaeaii? ? aeo?eioe? ia i?ioyc? 1 oa., ciaoiaeeoueny a
aeeaiacii? a?ae 0 aei 200 N.

5. Oaeaee?noue ioieiaeaeaiiy c 37 aei 00 N a ae?aiacii? a?ae 10 N/oa aei
960000N/oa ia ci?ith? caa?aaeaiino? aia??ii?a ??cieo noaae?e ?icaeoeo.

6. ??cia aeniiceoe?y aia??ii?a i?e 00 N (a?ae 7n aei 30 oa), ia ci?ith?
?o caa?aaeaiino? ia noaae?yo a?ae ?aiiuei? ii?oee aei ?icoe?aii?
aeanoioeenoe.

7. Iayai?noue a na?aaeiaeu? aeey caii?iaeoaaiiy e??ii?ioaeoi?a
(ae?oea?eio) ia ci?ith? caa?aaeai?noue aia??ii?a ??cieo noaae?e
?icaeoeo i?e ??ceiio ioieiaeaeaii?.

8. I?e aeeaieiio caii?iaeoaaii? aei oaiia?aoo?e ??aeeiai acioo o
noaiaea?oiiio ?aaeei? niinoa??aa?oueny caeaaei?noue caa?aaeaiino?
aia??ii?a a?ae noaae?? ?icaeoeo. Ca?iaeee ia noaae?yo a?ae eiiiaeoii?
ii?oee aei ?icoe?aieo aeanoioeeno aeeth/ii, iathoue iaeiaeiao
caa?aaeai?noue, yea eieeaa?oueny a?ae 73 aei 80%, aeaioaeiaai?
aeanoioeenoe ia ia?aiinyoue noaiaea?oi? ?aaeeie caii?iaeoaaiiy.

9. Ca?eueoaiiy aai ciaioaiiy oaeaeeino? caii?iaeoaaiiy a 2 ?ace o

ii??aiyii? c? noaiaea?oiei ?aaeeiii aeinoia??ii ia ci?ith?
caa?aaeaiino? ?aii?o ii?oe. Niinoa??aa?oueny oaiaeaioe?y c?inoaiiy
caa?aaeaiino? aeaioaeiaaieo aeanoioeeno (a?ae 0 aei 33%) i?e ciaioaii?
oaeaeeino? caii?iaeoaaiiy a?ae 0,6 aei 0,150 N / oa.

10. Ca?eueoaiiy eiioeaio?aoe?? ae?oea?eio a?ae 1,2 I aei 2,4I
i?ecaiaeeoue aei i?aeaeuaiiy caa?aaeaiino? aeaioaeiaaieo aeanoioeeno (
a?ae 14 aei 50%).

11. Caa?oath/e oaaao ia iai?ii? i?ioeane, an? aeanoiia?e 8-ie ee?oeiieo
aia??ii?a iathoue iaeiaeia? oiiae aeey o?ainii?oo ?a/iaei.

12. Aia??iie ia noaae?? ii?oee ?icae?eythoue ia aea? noaae??: ?aiiuei?
? eiiiaeoii? ii?oee, a caeaaeiino? a?ae e?eueeino? oa??a ?
eiiiaeoiino?, a ia noaae?? aeanoioeenoe- a caeaaeiino? a?ae ?ici??o
aeanoioeaey, iayaiino? ciie iaethoeeaea ? ?ioaineaiino? ??ciiiai?oieo
iai?i?a ?a/iaei.

13. An? aia??iie aei noaae?? 8-ie aeanoiia??a aeeth/ii aeioe?eueii
iaceaaoe ca caaaeueiith e?euee?noth ee?oei ( 1-ee?oeiia-ceaioa; 2-o;
4-o; e 8-ie ee?oeii?);

-noaae?th ii?oee ?icaeoe ia aea?: ?aiiy e eiiiaeoia ii?oea;

-noaae?th aeanoioeenoe ?icaeoe ia noaae??: ?aiiuei? aeanoioeenoe,
aeanoioeenoe, ui ?icoe?th?oueny, ?icoe?aii? aeanoioeenoe, aeanoioeenoe,
ui aeeoiey?oueny ?c ciie iaethoeeaea, a oaeiae aeaioaeiaaii?
aeanoioeenoe.

I?iiiceoe?? ae?iaieoeoao

1. Cai?iiiiiaaia iaie eeaneo?eaoe?y aia??ii?a ei?iae ??cieo noaae?e
?icaeoeo iiaea aooe aeei?enoaia o aocao aeey iaa/aiiy nooaeaio?a ?
i?aeaeuaiiy eaae?o?eaoe?? niaoe?ae?no?a, o ae?iaie/eo, a oaeiae a
iaoeiaeo i?ia?aiao.

2.I?e ?ic?iaoe? iaoiaeo caii?iaeoaaiiy aia??ii?a nnaaoe?a, ?aaeei
cieaeaiiy oaiia?aoo?e ? niin?a canoinoaaiiy e??i?ioaeoi?o iiaeii? aooe
i???ioiaai? ia aeanoiia?e, ui ciaoiaeyoueny a iaio ni?eyoeeaeo oiiaao
(aeeaiei?ici?uai?), a?aoiaoth/e iai?ii? i?ioeane.

3. Iioei?ciaaiee iaie ?aaeei cieaeaiiy oaiia?aoo?e (0,150 N / oa) ?
eiioeaio?aoe?y e??ii?ioaeoi?a (2,4I.) iiaeooue aooe canoiniaai? i?e
noai?aii? e??iaaieo aia??ii?a ei??a ia noaae?? ?icaeoeo aeaioaeiaaieo
aeanoioeeno c iaoith ?o iiaeaeueoiai aeei?enoaiiy a a?ioaoiieia??
a?aeoai?aiiy aaeeei? ?iaaoi? ooaeiae.

Nienie iioae?eiaaieo ?ia?o ii oai? aeena?oaoe??

1. Aacoaeue I.Ae. Iaonna O.N. Onoie/eainoue yia?eiiia ei?iau ?acee/iuo
noaaeee ?acaeoey e ?aceiio ioeaaeaeaieth // Iaoeiai-oaoi?/iee
atheeaoaiue ?75, Oa?e?a, 1998. -C.166-170. Caeiaoaa/ i?eeiaa o/anoue o
i?iaaaeaii? an??? aenia?eiaioaeueii? oa ?ic?aooieiai? /anoeie ?iaioe, a
a?iioace, ui iaa?oioiaothoue iaea?aeai? ?acoeueoaoe, aoee cai?iiiiiaai?
iaoeiaei ea??aieeii.

2. Iaonna O.N. Iniaaiiinoe o?ainii?oa aiaeu e ?anoai?aiieo aauanoa a e
ec yia?eiiia ei?ia ?acee/ieo noaaeee ?acaeoey // Iaoeiai-oaoi?/iee
atheeaoaiue ?75, Oa?e?a. -1998. -C.171-174.

3. Iaonna O.N. Eciaiaiea nio?aiiinoe yia?eiiia ei?iau ?aiieo noaaeee
?acaeoey i?e ?aciuo nei?inoyo ioeaaeaeaiey e a?aiaiao eo yeniiceoeee i?e
00N // I?iaeaie Cii?iaeaia??? oa Aaoa?eia?ii? Iaaeeoeeie ? 4, Oa?e?a.
-1998. -N.68-70.

4. Iaonna O.N Iaaeaaaeianeee A.A. Eeneeue A.A. E aii?ino i
eeanneoeeaoeee i?aaeeiieaioaoeeiiiuo yia?eiiia ei?iau //
Iaoeiai-oaoi?/iee atheeaoaiue ?75, Oa?e?a. -1998. -C.175-178.
Oaeineiiaeaiiy i?eioeeia eeaneo?eaoe?? aia??ii?a ei??a aoei
cai?iiiiiaaii aaoi?ii iniaenoi. A iaaiai?aiiy oeueiai i?eioeeio
i?iaaaeaii eieaeoeaii ni?aaaoi??a.

5. Iaonna O.N. Eia?eiiae/ O.I. Caii?aaeeaaiea yia?eiiia ei?iau ia
noaaeeyo ?aiieo ii?oe e aeaioaee?iaaiiuo aeanoioeeno // ‘’A?niee I?iaeai
A?ieia?? ? Maaeeoeeie’’ ? 10, Oe?a?inueea Iaaee/ia Noiiaoieia?/ia
Aeaaeai?y.-1999. -C.24-28. Caeiaoaa/ii oa ni?aaaoi?ii i?iaaaeaii
caii?aaeoaaiiy aia??ii?a ei??a ? iaaiai?aiiy ?o ?acoeueoao?a.

6. Iaonna O.N. Onoie/eainoue yia?eiiia e?oiiiai ?iaaoiai neioa e
aicaeaenoaeth ieceeo oaiia?aoo? // Oai?ey e i?aeoeea ieaiaiiai aeaea a
aeeaioiiaiaenoaa. Iaoa?eaeu iaaeaeoia?iaeiie iao/ii-i?aeoe/aneie
eiioa?aioeee, iinayuaiiie 80-eaoeth ni aeiy ?iaeaeaiey
/eaia-ei??aniiiaeaioa AANOIEE Yenia?a Oaaei?a Oaaei?iae/a, Oa?e?a.
-1996. -N.70-71.

Annotation

Mausse Francisco Salomao. Cryosensitivity of bovine embryos on its
different developmental stages. — Manuscript.

Thesis for a Ph.D. degree in agrarian sciences by speciality 03.00.20 —
biotechnology — postgraduate of the institute of Animal Science of UAAS,
Kharkov, 1999.

Dissertation work served the development of bovine embryos
cryopreservation studies, creation of effective cryopreservative methods
for embryos of later stages.

On the basis of obtained results was established, that after hormonal
cow treatment for superovulation, its flushing results on high age
embryos, disparity, which is probably caused by unsynchronized process
of ovulation and different fertility length of cows. There was
established too, that embryos, cryoresistance rises on the dependence of
their age; the level of substance exchange between embryos and their
surounding environment differs according to their age, fact which has to
be taken under consideration by specialists.

There was optimized cryopreservative regimen for 9-10 days embryos.
There was propoused a new system of embryos, classification according to
their age and level of substance exchanges. Basic results of the work
received practical attachment for the management in the above mentioned
institution and in the Ahtirskii laboratory for embryo transplantation.

Key words: cow embryo, different developmental stages,
cryopreservation.

AIIOAOe?ss

Iaonna O?ain?nei Naeiiii. ‘’*ooeea?noue aia??ii?a aaeeei? ?iaaoi?
ooaeiae ??cieo noaae?e ?icaeoeo aei ae?? iecueeeo oaiia?aoo?‘’.
-?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa
n?euenueeiainiiaea?nueeeo iaoe ca niaoe?aeuei?noth 03.00.20-
a?ioaoiieia?y. -?inoeooo oaa?eiieoeoaa OAAI, Oa?e?a, 1999. Aeena?oaoe?y
i?enay/aia aea/aiith /ooeeaino? aei ae?? iecueeeo oaiia?aoo? aia??ii?a
ei??a ??cieo noaae?e ?icaeoeo ? ?ic?iaoe? ?aaeei?a caii?iaeoaaiiy
aia??ii?a i?ci?o noaae?e ?icaeoeo. Anoaiiaeaii, ui i?e aeieaaii?
ia?iaeaieo c iaoith io?eiaiiy ?aaeoe?? noia?iaoeyoe?? ei??a,
niinoa??aa?oueny aeniea a?eiaa aaoa?iaaii?noue iiioeyoe?e aia??ii?a,
io?eiaieo ?c noaoaaeo oeyo?a ei??a ia i?ioyc? iaeiiai noaoaaiai oeeeeo.

No?ee?noue aia??ii?a ei??a ??cieo noaae?e ?icaeoeo aei ae?? iecueeeo
oaiia?aoo? c a?eii c?inoa?. ??aaiue iai?io aiaee ? ?ic/eiaieo a i?e
?a/iaei ??ciee aeey aia??ii?a ei??a ??cieo noaae?e ?icaeoeo, ui iiaeii
aooe a?aoiaaii a?ioaoiieiaaie. Aoee iioei?ciaai? ?aaeeie caii?iaeoaaiiy
aia??ii?a ia noaae?? aeaioaeiaaieo aeanoioeeno. Aoea cai?iiiiiaaia iiaa
eeaneo?eaoe?y aia??ii?a o caeaaei?no? a?ae noaae?e ?icaeoeo ?
a?aeiia?aeiiai ??aiy iai?iieo i?ioean?a.

?acoeueoaoe ?iaioe ciaeoee canoinoaaiiy o aeuaaeacaiiio ?inoeooo? ? a
Iooe?nuee?e eaai?aoi??? o?ainieaioaoe?? aia??ii?a.

Eeth/ia? neiaa: aia??iie ei??a, ??ci? noaae?? ?icaeoeo,
caii?iaeoaaiiy.

AIIIOAOeEss

Iaonna O?ainenei Naeiiii. ‘’ *oanoaeoaeueiinoue yia?eiiia e?oiiiai
?iaaoiai neioa ?aciuo noaaeee ?acaeoey e aicaeaenoaeth ieceeo
oaiia?aoo?‘’. -?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa
naeueneioicyenoaaiiuo iaoe ii niaoeeaeueiinoe 03.00.20- aeioaoiieiaey. —
Einoeooo AEeaioiiaiaenoaa OAAI, Oa?ueeia, 1998. Aeenna?oaoeey iinayuaia
eco/aieth /oanoaeoaeueiinoe e aicaeaenoaeth ieceeo oaiia?aoo? yia?eiiia
ei?ia ?aciuo noaaeee ?acaeoey e oniaa?oainoaiaaieth ?aaeeiia
caii?aaeeaaiey yia?eiiia ia noaaeeyo iicaeieo ii?oe ?aiieo e
aeanoioeeno, aeey caii?aaeeaaiey yia?eiiia ?aiieo ii?oe e
aeaioaee?iaaiiuo aeanoioeeno, oniaa?oainoaiaaieth nenoaiu eeanneoeeaoeee
i?aaeeiieaioaoeeiiiuo yia?eiiia ei?ia.

Aeaiiay ?aaioa iniiaaia ia oii oaeoa, /oi nia?aiaiiay aeioaoiieiaey
aini?iecaaaeaiey naeueneioicyenoaaiiuo aeeaioiuo i?aaeiieaaaao
eniieueciaaiea yia?eiiia ei?ia ?acee/iuo noaaeee ?acaeoey. E?iia oiai
yooaeoeaiinoue i?eiaiaiey iiaaeoeo iaoiaeia ?aciiiaeaiey aeeaioiuo
(ii?aaeaeaiea iiea, iieo/aiea oeia?, yia?eiiaeueiuo eeiiia,
o?aaeeoeeiiiay o?ainieaioaoeey yia?eiiia e ae?.) aic?anoaao n o/aoii
iaee/ey aaiea caii?iaeaiiuo yia?eiiia.

Caii?aaeeaaiea yia?eiiia yoiai aeaea aeeaioiuo ia noaaeeyo ?aiieo ii?oe
e aeaioaee?iaaiiuo aeanoioeeno n eniieueciaaieai o?aaeeoeeiiiuo
iaoiaeia, auei i?iaaaeaii ?aciuie aaoi?aie. Iieo/aiiua eie ?acoeueoaou
iieacaee ieceee o?iaaiue nio?aiiinoe caii?iaeaiii-iooayiiuo yia?eiiia,
ii n?aaiaieth n oaeiauie, iieo/aiiuie i?e caii?aaeeaaiee yia?eiiia
iicaeieo ii?oe e ?aiieo aeanoioeeno (6,5-8 nooi/iua aeanoioeenou).

I?e caii?aaeeaaiee yia?eiiia ia noaaeeyo aeaioaee?iaaiiuo aeanoioeeno n
eniieueciaaieai noaiaea?oiuo iaoiaeia, yia?eiiu ia oniaaatho i?ieoe
i?ioeann aeaaeae?aoaoeee e ia?eiaeo oi?ie?iaaieth aioo?eeeaoi/iuo
e?enoaeeia eueaea. Yoi yaeaiea i?eaiaeeo e ia?aciaaieth ?aciuo oi?i
e?enoaeeia eueaea aioo?e eeaoie, /oi i?eaiaeeo e eeceno yia?eiia ai
a?aiy iooaeaaiey, oae eae i?e yoii eiaao ianoi oae iacuaaaiue i?ioeann
?ae?enoaeeecaoeee.

I?e/eia ieceie nio?aiiinoe oi/ii ia ii?aaeaeaia, ii i?aaeiieaaaaony, /oi
aeioeiey, oeceieiaey e niiniaiinoe caii?aaeeaaaiuo ca?iaeuoae ?aciuo
noaaeee ?acaeoey e aeaaeae?aoaoeeth, ea?atho nouanoaaiioth ?ieue a eo
ioaaoiie ?aaeoeee ia aicaeaenoaea ieceeo oaiia?aoo?. Aeaiiua aeiioacu
auoaeatho ec-ca oiai, /oi /oanoaeoaeueiinoue yia?eiiia ?aciuo noaaeee
?acaeoey e ioeaaeaeaieth e caii?aaeeaaieth ioee/aaony.

?aiaa ?ac?aaioaiiua iaoiaeu caii?aaeeaaiey yia?eiiia ieaeiieoathueo
yooaeoeaii i?eiaiythony no?iai aeey caii?aaeeaaiey yia?eiiia
ii?aaeaeaiiuo noaaeee ?acaeoey.

A nayce n oai, /oi a ?aciuo eaai?aoi?eyo, caieiathueony iaieioeyoeeae n
yia?eiiaie, eniieuecoaony ii?aaeaeaiiay nenoaia eeanneoeeaoeee
yia?eiiia, aicieeea iaiaoiaeeiinoue ooi/ieoue nenoaio eeanneoeeaoeee,
i?eieiay ca iniiao ?aiaa i?iaaaeaiioth ae?oaeie aaoi?aie. Aaaeii
ioiaoeoue, /oi i?iaaaeaia iaie eeanneoeeaoeey auea i?aaeiieaaaaiay n
o/aoii aic?anoa yia?eiiia e eioaineaiinoe i?ioaeaiey iaiaia aiaeu e
e?eii?ioaeoi?a. Eeanneoeeaoeey yia?eiiia n o/aoii o?iaiy i?ioaeaiey
iniioe/aneeo i?ioeannia eiaao aieueoia cia/aiea i?e i?iaaaeaiee eo
caii?aaeeaaiey, oae eae i?iaiice?iaaoue o?iaaiue nio?aiiinoe e
i?aaeeaaaoue ?aaeeiu eo caii?aaeeaaiey aiciiaeii oieueei n o/aoii eo
aic?anoia.

Onoaiiaeaii, /oi i?e auiuaaiee yia?eiiia aeiii?ia, ia?aaioaiiuo ia
noia?iaoeyoeeth, iaaethaeaaony aunieay aic?anoiay aaoa?iaaiiinoue
iiioeyoeee yia?eiiia, iieo/aiiuo ec iieiauo iooae ei?ia ia i?ioyaeaiee
iaeiiai iieiaiai oeeeea; onoie/eainoue yia?eiiia ?aciuo noaaeee ?acaeoey
e aicaeaenoaeth ieceeo oaiia?aoo? aic?anoaao n aic?anoii. O?iaaiue
i?ioaeaiey iaiaia aiaeu e ?anoai?aiiuo a iae aauanoa (e?eii?ioaeoi?)
?aciue aeey yia?eiiia ei?ia ?aciuo noaaeee ?acaeoey, /oi aeieaeii auoue
o/oaii aeioaoiieiaaie i?e i?aaeaa?eoaeueiii i?iaiice?iaaiee ?acoeueoaoia
caii?aaeeaaiey yia?eiiia. Onoaiiaeaii, /oi caii?aaeeaaiea yia?eiiia ia
noaaeee aeaioaee?iaaiiuo aeanoioeeno ni nei?inoue 0,150N/i e 2,4I
aeeoea?eia a ea/anoaa e?eii?ioaeoi?a, iiaeao iiauneoue o?iaaiue
nio?aiiinoe ia 50%. Oaeei ia?acii, yoio ?aaeei caii?aaeeaaiey yaeyaony
iioeiaeueiui aeey yia?eiiia aeaioaee?iaaiiuo aeanoioeeno. Auea
i?aaeeiaeaia iiaay eeanneoeeaoeey yia?eiiia a caaeneiinoe io noaaeee
?acaeoey e niioaaonoaothuaai o?iaiy i?ioaeaiey iniioe/aneeo i?ioeannia.

Caii?aaeeaaiea yia?eiiia ia noaaeee ?aiieo ii?oe ia ?acoeueoaoeaii i?e
anao iiiuoeao iaie i?iaaaeaiu: ie oaaee/aiea eiioeaio?aoeee
e?eii?ioaeoi?a (1,2I, 1,8I e 2,4I), ie nieaeaiea nei?inoae
caii?aaeeaaiey (0,60N/iei, 0,30N/iei e 0,150N/iei ) ia i?eaaee e
iiauoaieth o?iaiy eo nio?aiiinoe. I/aaeaeii, aeey ?acoeueoaoeaiiai
caii?aaeeaaiey yia?eiiia yoeo noaaeee ?acaeoey iaiaoiaeeii i?eiaiyoue
ae?oaea iao?aaeeoeeiiiua iiaeoiaeu.

?acoeueoaou ?aaiou iaoee i?eiaiaiea a auoaoeacaiiii einoeoooa e a
Aoou?neie eaai?aoi?ee o?ainieaioaoeee yia?eiiia. Eeth/aaua neiaa:
yia?eiiu ei?ia, ?aciua noaaeee ?acaeoey, caii?aaeeaaiea.

Aiioaoe?y

Iaonna O?ainenei Naeiiii. “*ooeea?noue aia??ii?a aaeeei? ?iaaoi? ooaeiae
??cieo noaae?e ?icaeoeo aei ae?? iecueeeo oaiia?aoo?. – ?oeiien.

Aeena?oaoe?y ia caeiaoooy iaoeiaiai nooiaiiy eaiaeeaeaoa
n?euenueeiainiiaea?nueeeo iaoe ca niaoe?aeuei?noth 03.00.20 –
a?ioaoiieia?y. – ?inoeooo oaa?eiieoeoaa OAAI, Oa?e?a, 1999.

Aeena?oaoe?y ienay/aia aea/aiiy /ooeeaino? aei ae?? iecueeeo oaiia?aoo?
aia??ii?a ei??a ??cieo noaae?e ?icaeoeo ? ?ic?iaoe? ?aaeei?a
caii?iaeoaaiiy aia??ii?a ??ci?o noaae?e ?icaeoeo. Anoaiiaeaii, ui i?e
aeieaaii? ii?iaeaieo c iaoith io?eiaiiy ?aaeoe?? noia?aioeyoe?? ei??a,
niinoa?eaa?ouey aeniea a?eia aaoa?iaaii?noue iiioeyoe?e aia??ii?a,
io?eiaieo ?c noaoaaeo oeyo?a ei??a ia i?ioyc? iaeiiai noaoaaiai oeeeeo.

No?ee?noue aia??ii?a ??cieo noaae?e ?icaeoeo aei ae?? iecueeeo
oaiia?aoo? c aeeii c?inoa?. ??aaiue iai?io aiaee ? ?ic/eiaieo a i?e
?a/iaei ??ciee aeey aia??ii?a ei??a ??cieo noaae?e ?icaeoeo, ui iiaeaeii
aoo? a?aoiaaii a?ioaoiieiaai. Aoee iioei?ciaai? ?aaeeie caii?iaeoaaiiy
aia??ii?a ia noaae?? aeaioaeiaaieo aeanoioeeno. Aoea cai?iiiiiaaia iiaa
eeaneo?eaoe?y aia??ii?a o caeaaei?no? a?ae noaae?e ?icaeoeo ?
a?aeiia?aeiiai ??aiy iai?iieo i?ioean?a.

?acoeueoaoe ?iaioe ciaoee canoinoaaiiy o aeuaaeacaiiio ?inoeooo? ? a
Iooe?nuee?e eaai?aoi??? o?ainieaioaoe?? aia??ii?a.

AIIIOAOeEss

Iaonna O?ainenei Naeiiii. “*oanoaeoaeueiinoue yia?eiiia e?oiiiai
?iaaoiai neioa ?aciuo noaaeee ?acaeoey e aicaeaenoaeth ieceeo
oaiia?aoo?”. -?oeiienue.

Aeenna?oaoeey ia nieneaiea o/aiie noaiaie eaiaeeaeaoa
naeueneioicyenoaaiiuo iaoe ii niaoeeaeueiinoe 03.00.20 – aeioaoiieiaey.
– Einoeooo aeeaioiiaiaenoaa OAAI, Oa?ueeia, 1999. Aeennna?oaoeey
iinayuaia eco/aieth /oanoaeoaeueiinoe e aicaeaenoaeth ieceeo oaiia?aoo?
yia?eiiia ei?ia ?aciuo noaaeee ?acaeoey e oniaa?oainoiaaieth ?aaeeiia
caii?aaeeaaiey yia?eiiia ia noaaeeyo iicaeiuo ii?oe e ?aiieo
aeanoioeeno, aeey caii?aaeeaaiey yia?eiiia ?aiieo ii?oe e
aeaioaee?iaaiiuo aeanoioeeno; oniaa?oainoaiaaiea nenoaio eeanneoeeaoeee
i?aaeeiieaioaoeeiiiuo yia?eiiia ei?ia.

Aeaiiay ?aaioa iniiaaia ia oii, /oi nia?aiaiiay aeioaoiieiaey
aini?iecaaaeaiey naeueneioicyenoaaiiuo aeeaioiuo i?aaeiieaaaao
eniieueciaaiea yia?eiiia ei?ia ?acee/iuo noaaeee ?acaeoey. E?iia oiai
yoaeoeaiinoue i?eiaiaiey iiaaeoeo iaoiaeia ?aciiiaeaiey aeeaioiuo
(ii?aaeaeaiea iiea, iieo/aiea oeia?, yia?eiiaeueiuo eeiiia,
o?aaeeoeiiiay o?ainieaioaoeey yia?eiiia e ae?.) aic?anoaao n o/aoii
iaee/ey aaiea caii?iaeaiiuo yia?eiiia.

Caii?aaeeaaiea yia?eiiia yoiai aeaea aeeaioiuo ia noaaeeyo ?aiieo ii?oe
e aeaioaee?iaaiiuo aeanoioeeno n eniieueciaaieai o?aaeeoeeiiiuo
iaoiaeia, auei i?iaaaeaii ?aciuie aaoi?aie. Iieo/aiiua eie ?acoeueoaou
iieacaee ieceee o?iaaiue nio?aiiinoe caii?iaeaiii-iooayiiuo yia?eiiia,
ii n?aaiaieth n oaeiauie, iieo/aiiuie i?e caii?aaeeaaiee yia?eiiia
iicaeiuo ii?oe e ?aiieo aeanoioeeno (6,5 –8 nooi/iua aeanoioeenou).

I?e caii?aaeeaaiee yia?eiiia ia noaaeeyo aeaioaee?iaaiiuo aeanoioeeno n
eniieueciaaieai noaiaea?oiuo iaoiaeia, yia?eiiu ia oniaaatho i?ieoe
i?ioeann aeaaeae?aoaoeee e ia?eiaeo oi?ie?iaaiey aioo?ee?enoaeeia
eueaea. Yoi yaeaiea i?eaiaeeo e oi?ie?iaaieth ?aciuo oi?i e?enoaeeia
eueaea aioo?e eeaoie, /oi caeai/eaaaony eecenii yia?eiia ai a?aiy
iooaeaaiey, oae eae i?e yoii eiaao ianoi oae iacuaaaiue i?ioeann
?ae?enoaeeecaoeee.

I?e/eia iieo/aiey ieceie nio?aiiinoe oi/ii ia ii?aaeaeaia, ii
i?aaeiieaaaaony, /oi aeioeiey, oeceieiaey e niiniaiinoe caii?aaeeaaaiuo
ca?iaeuoae ?aciuo noaaeee ?acaeoey e aeaaeae?aoaoeeth, ea?atho
nouanoaaiioth ?ieue a eo ioaaoioth ?aaeoeeth ia aicaeaenoaea ieceeo
oaiia?aoo?. Aeaiiua aeiioacu auoaeatho ec-ca oiai, /oi
/oanoaeoaeueiinoue yia?eiiia ?aciuo noaaeee ?acaeoey e ioeaaeaeaieth e
caii?aaeeaaieth ioee/aaony.

?aiaa ?ac?aaioaiiua iaoiaeu caii?aaeeaaiey yia?eiiia ieaeiieoathueo
yooaeoeaii i?eiaiythony no?iai aeey caii?aaeeaaiey yia?eiiia
ii?aaeaeaiiuo noaaeee ?acaeoey.

A nayce n oai, /oi a ?aciuo eaai?aoi?eyo caieiathueony iaieioeyoeeae n
yia?eiiaie, eniieuecoaoey ii?aaeaeaiiay nenoaia eeanneoeeaoeee
yia?eiiia, aicieeea iaiaoiaeeiinoue ooi/ieoue nenoaio eeanneoeeaoeee,
i?eieiay ca iniiao?aiia i?iaaaeaiioth ae?oaeie aaoi?aie. Aaaeii
ioiaoeoue, /oi i?iaaaeaia iaie eeanneoeeaoeey auea i?aaeiieaaaaiay n
o/aoii aic?anoa yia?eiiia, e eioaioeeaiinoe i?ioaeaiey iaiaia aiaeu e
e?eii?ioaeoi?a. Eeanneoeeaoeey yia?eiiia n o/aoii o?iaiy i?ioaeaiey
iniioe/aneeo i?ioeannia eiaao aieueoia cia/aiea i?e i?iaaaeaiee eo
caii?aaeeaaiey, oae eae i?iaiice?iaaoue o?iaaiue e i?aaeeaaaoue ?aaeeiu
eo caii?aaeeaaiey aiciiaeii oieueei n o/aoii yoiai ia?aiao?a.

Onoaiiaeaii, /oi i?e auiuaaiee yia?eiiia aeiii?ia, ia?aaioaiiuo ia
noia?iaoeyoeeth, iaaethaeaaony aunieay aic?anoiay aaoa?iaaiiinoue
iiioeyoeee yia?eiiia, iieo/aiiuo ec iieiauo iooae ei?ia ia i?ioyaeaiee
iaeiiai iieiaiai oeeeea; onoie/eainoue yia?eiiia ?aciuo noaaeee ?acaeoey
e aicaeaenoaeth ieceeo oaiia?aoo? aic?anoaao n aic?anoii. O?iaaiue
i?ioaeaiey iaiaia aiaeu e ?anoai?aiiuo a iae aaouanoa (e?eii?ioaeoi?)
?aciue aeey yia?eiiia ?aciuo noaaeee ?acaeoey, /oi aeieaeai auoue o/oaii
aeioaoiieiaaie i?e i?aaeaa?eoaeueiii i?iaiice?iaaiee ?acoeueoaoia
caii?aaeeaaiee yia?eiiia. Onoaiiaeaii, /oi caii?aaeeaaiea yia?eiiia ia
noaaeee aeaioaee?iaaiiuo aeanoioeeno ni nei?inoueth 0,150N/iei e 2,4 I
aeeoea?eia a ea/anoaa e?eii?ioaeoi?a iiaeao iiauneoue o?iaiy nio?aiiinoe
ia 50%. Oaeei ia?acii, yoio ?aaeei caii?aaeeaaiey yaeaaony iioeiaeueiui
aeey yia?eiiia ia noaaeee aeaioaee?iaaiiuo aeanoioeeno.

Caii?aaeeaaiea yia?eiiia ia noaaeee ?aiieo ii?oe ia ?acoeueoaoeaii i?e
anao iiiuoeao iaie i?iaaaeaiu: ie oaaee/aiea eiioeaio?aoeee
e?eii?ioaeoi?a (1,2 I; 1,8I e 2,4I), ie nieaeaiea nei?inoae
caii?aaeeaaiey (0,60N/iei, 0,3N0/iei e 0,15N0/iei) ia i?eaaee e
iiauoaieth o?iaiy eo nio?aiiinoe. I/aaeaeii, /oi aeey ?acoeueoaoeaiiai
caii?aaeeaaiey yia?eiiia yoeo noaaeee ?acaeoey iaiaoiaeeii i?eiaiyoue
ae?oaea iao?aaeeoeeiiiua iiaeoiaeu.

?acoeueoaou ?aaiou iaoee i?eiaiaiea a auoaoeacaiiii einoeoooa e a
Aoou?neie eaai?aoi?ee ii o?ainieaioaoeee yia?eiiia.

ANNOTATION

Mausse Francisco Salomao. Cryosensitivityof bovine embryos on its
different developmental stages. – Manuscript.

Thesis for a Ph.D. degree in agrarian sciences by speciality 03.00.20 –
biotechnology – posgraduate of the institute of Animal Science under the
power of UAAS, Kharkov, 1999.

Dissertation work served the development of bovine embryos
cryopreservation studies, creation of effective cryopreservative methods
for embryos of late stages.

On the basis of obtained results was established, that after hormonal
cow treatment for superovulation, its flushing results on high age
embryo disparity,which is probably caused by unsynchronized process of
ovulation and diffrent fertility lenght of cows. There was established
too, that embryos’ cryoresistance rises on the dependence of their age,
the level of substance exchange between embryos and their sorounding
environment differs according to their age, fact which has to be taken
under consideration by specialists.

There was optimized cryopreservative regimen for days 9-10 embryos.
There was propoused a new system of embryos’ classification according to
their age and level of substance exchanges. Basic results of the work
has recieved practical attachment for the management in the above
mentioned institution and in the Ahtirskii laboratory for embryo
transplantation.

I?aeienaii aei ae?oeo 12.05.1999

Ianya1,0 ae.a. Oe?aae 100 ?ec.

?ioii?eio ?inoeoooo oaa?eiieoeoaa, Oa?ueeia, i/i Eoeeie/?, 312120

oae. 95-31-81

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