Біологічні особливості Melilotus officinalis (L.) Pall. при вегетативному розмноженні: Автореф. дис… канд. біол. наук / В.Є. Харченко, НАН України.

6

IAOe?IIAEUeIA AEAAeAI?ss IAOE OE?A?IE

OeAIO?AEUeIEE AIOAI?*IEE NAAe

IIAI? I.I.A?EOEA

Oa?/aiei Aieoi?iy ?aaaiiaia

OAeE: 581.522.4: 580:633.366:631.532

A?IEIA?*I? INIAEEAINO? MELILOTUS OFFICINALIS (L.)PALL. I?E AAAAOAOEAIIIO
?ICIIIAEAII?

03.00.05. — aioai?ea

Aaoi?aoa?ao

aeena?oaoei? ia caeiaoooy iaoeiaiai nooiaiy

eaiaeeaeaoa aiieia?/ieo iaoe

Ee?a – 1999

Aeena?oaoei?th ? ?oeiien.

?iaioa aeeiiaia o Eoaainueeiio nieuenueeiainiiaea?nueeiio oiiaa?neoao?.

Iaoeiaee ea?iaiee: aeieoi? aiieiai/ieo iaoe, i?ioani?

Nieieia Iaai Aeieo?iae/

Eoaainueeee aea?aeaaiee aa?a?iee oiiaa?neoao, caaiaeoth/ee eaoaae?ith
aioaiiee oa aeieiai?

Ioioeieii iiiiaioe: aeieoi? a?ieia?/ieo iaoe,

C?iai Na?oeaia Ieeiea?aia

?inoeooo aioai?ee ?i. I.A. Oieiaeiiai IAI Oe?a?ie, aieiaiee iaoeiaee
ni?a?ia?oiee

eaiaeeaeao a?ieia?/ieo iaoe

Aoeao Iao?i ?aaai?eiae/

Oeaio?aeueiee aioai?/iee naae ?i. I.I.A?eoea IAI Oe?a?ie, noa?oee
iaoeiaee ni?a?ia?oiee

I?iaiaeia onoaiiaa: Iaoe?iiaeueiee aa?a?iee oi?aa?neoao, eaoaae?a
aioai?ee,i. Ee?a.

Caoeno aiaeaoaeaoueny “5”eenoiaaea 1999 ?. i _13.00. aiaeeii ia
caniaeaiii niaoeiaeiciaaii? a/aii? ?aaee E 26.215.01 aeey caeiaoooy
iaoeiaiai nooiaiy eaiaeeaeaoa a?ieia?/ieo iaoe i?e Oeaio?aeueiiio
aioai?/iiio naae? IAI Oe?a?ie ?iai? I.I. A?eoea IAI Oe?a?ie ca
aae?anith:

252014, i. Ee?a, aoe. O?i??yc?anueea, 1.

C aeena?oaoei?th iiaeia iciaeiieoenue o aiaeiioaoei Oeaio?aeueiiai
aioai?/iiai naaeo ?iai? I.I. A?eoea IAI Oe?a?ie,

252014, i.Ee??a, aoe. O?i??yc?anueea, 1.

Aaoi?aoa?ao ?icineaiee “27“ aa?aniy 1999 ?.

A/aiee nae?aoa?

niaoeiaeiciaaii? a/aii? ?aaee

eaiaeeaeao a?ieia?/ieo iaoe
Ioce/oe A.I.

CAAAEUeIA OA?AEOA?ENOEEA ?IAIOE

Aeooaeuei?noue oaie. A Oe?a?i? ?nio? ?yae i?iaeai aeiiii?/iiai oa
aeieia?/iiai oa?aeoa?o, iaeiei ?c iai?yie?a ae??oaiiy yeeo ?
aeei?enoaiiy o ainiiaea?noaao iiaeo ia?niaeoeaieo eoeueoo?. Ialilotus
officinalis (L.)Pall. — oe?iia ei?iiaa, o?oiiae?i?aoeaia,
ia?icai?ithth/a, aioe?aae?iaeoeaia, iaaeiiinia oa e?ea?nueea ?ineeia
(A?otheia, 1973, Iieueiaee, 1989). O eoeueoo?? M. officinalis ca
ei?ioeee oa?i?i iiaeia io?eiaoe aenie? noaa?euei? a?iaea? ei?iiai? iane
oa ian?iiy, e??i oiai ?ineeie iiaeooue aooe aeei?enoai? ia caeaiee ei?i
oa aiaeii/an ae?thoue, ye aeia?eai, ai caeaoi? iaeiie/oaaoe acio (aei
150-200 ea/aa) (Noai?ia, 1962, Niaoaiei, 1987). Ai?no i?ioa?io a
ei?iia?e ian? ia nooo ?a/iaeio noaiiaeoue 160 — 190 a/ea (Ianaeeiia,
1991). E??i oiai, oea aenieia?oai?iia ?ineeia. Oae, o ia?a?aooieo ia
nooo ?a/iaeio ai?no anei?a?iiai? eeneioe neeaaea? 827 ia/100a., ea?ioeio
— 199 iea/a., a?oai?io A — 205 iea/ a, a?oai?i?a A1 — 9 iea/a, oa A2 — 1
iea/a (Aiaioiaa, 1983). M. officinalis iaaeaaaeeaee aei ?iaeth/ino?
a?oio?a, ia?aoeiaeaea? ?o a?ic??, yea ia oa?eoi??? Oe?a?ie ui??/ii
ca?eueoo?oueny ia 100 – 120 oen. aa (Oeeoea, 1998). Iaeiae
?iciianthaeaeaiiy M. officinalis o ainiiaea?noaao e?a?ie iaaeoi
iaiaaeaia, ui iiynith?oueny aeao?oeeoii ian?iiy oa iaaeineiiae?noth
?aeiiiaaieo ni?o?a, ye? iathoue ?yae iaaeie?e?a: a?oa? noaaea, iecueeo
iaeenoiai?noue oa ?i. (Naaaeaaeia, 1984).

Naeaeoe?ei? ?iaioe no?eiothoueny iaaeinoaoiueith aea/ai?noth
a?ieia?/ieo iniaeeaioae oe??? ?ineeie. E??i oiai M. officinalis ?
aeai??/iei enaiiaaiiei aioiiio?eii, e a?eueo?noue c?ace?a ?c caaeaieie
iciaeaie iinooiiai ao?a/a? ?o /anoeio o ca’yceo c ia?ao?aniei
caieeaiiyi c i?e?iaeieie oi?iaie. Oaeei /eiii, aeiiii?/ii aeioe?eueiei
aoei i?iaaaeaiiy ?ia?o, ni?yiiaaieo ia i?enei?aia ca?eueoaiiy a
iiioeyoe?yo e?eueeino? ?ineei c oe?iieie iciaeaie oa i?aeaeuaiiy
eiia??iino? ooai?aiiy iiaeo oi?i oa ni?o?a.

Aaaaoaoeaia ?iciiiaeaiiy aeicaiey? oaeaeei io?eioaaoe aaeeeo e?euee?noue
?ineei aac ci?ie aaiioeio. Aeey aeayeeo i?aaenoaaiee?a ?iaeeie Fabaceaa
(Madicago sativa, Vic?a faba oa ?i.) aaea ?ic?iaeai? iaoiaeeee
aaaaoaoeaiiai ?iciiiaeaiiy (Ianoieaoee, 1970, Iiaiaeeeia, 1987). Oiio
aeey i?enei?aiiy naeaeoe?? M. officinal?s aeioe?eueiei aoei i?iaaaeaiiy
iaoeiaeo aeine?aeaeaiue ii aea/aiith a?ieia?/ieo iniaeeainoae oe???
?ineeie i?e aaaaoaoaiiio ?iciiiaeaii?. Caaaa/aiiy ni?oiaiai ani?oeiaioo
M. officinal?s ia? caaania/eoe ca?eueoaiiy iin?aii? ieiu?, i?ae oe??th
eoeueoo?ith, ui ni?eyoeia iie?ioaiith aeieia?/iiai noaio a?oio?a
Oe?a?ie ca ?aooiie cieaeaiiy ?ioaineaiino? a?ic?eieo i?ioean?a, oa ??aiy
?oiuei? ?aae?iaeoeaiino?, ca?eueoaiith ae?iaieoeoaa ?ineeiiiai a?eea
oiui.

Ca’ycie ?iaioe c iaoeiaeie i?ia?aiaie, ieaiaie, oaiaie. Aeine?aeaeaiiy c
ieoaiue aea/aiiy a?ieia?/ieo iniaeeainoae aaaaoaoeaiiai ?iciiiaeaiiy M.
officinal?s aoei neeaaeiaith /anoeiith oaiaoe/iiai ieaio eaoaae?e
aioai?ee oa aeieia?? Eoaainueeiai aea?aeaaiia aa?a?iiai oi?aa?neoaoo ii
aeeiiaiith aea?aeaaiiai ieaio a 1991-1999 ?.?. i?iaeoo “Ei?ie ? ei?iiaee
a?eie» IOI “I?iaeiaieuenoai-95“ (iiia? aea?aeaaii? ?a?no?aoe?? UAO
1002374 P).

Iaoa ? caaea/? aeine?aeaeaiiy. Iaoa aeine?aeaeaiue iieyaaea o ?ic?iaoe?
iaoiae?a i?enei?aiiai ca?eueoaiiy a iiioeyoe?yo M. officinal?s
e?eueeino? oi?i c oe?ieie iciaeaie ia iniia? aaaaoaoeaiiai ?iciiiaeaiiy,
a?aoiaoth/e iniaeeaino? iioiaaiaco aeaii? ?ineeie, aeey i?aeaeuaiiy
eiia??iino? noai?aiiy oa caa?aaeaiiy ni?o?a c aaaeaieie iciaeaie.

?aae?caoe?y iinoaaeaii? iaoe aeiaaaea ?ica’ycaiiy oaeeo caaaeaiue:
aeyaeoe iniaeeaino? naciiiiai ?icaeoeo eieaeoe?eieo ni?oi-c?ace?a a
oiiaao oeaio?aeueii? oa i?aaeaiii-no?aeii? Oe?a?ie; aea/eoe iniaeeaino?
oei??iaiiy aeeaoe?a oa oeyoe iioei?caoe?? oeueiai i?ioeano;
i?iaiae?coaaoe ei?aeyoe?th iciae, ui oa?aeoa?ecothoue ei?aiaooai?thaaiiy
ia aeeaoeyo; aea/eoe ?icaeoie ?ineei, ui aoee ?iciiiaeai? aaaaoaoeaii;
aecia/eoe iiaeeeaino? ?ioaineo?eaoe?? aaaaoaoeaiiai ?iciiiaeaiiy ca
?aooiie canoinoaaiiy aeiooaiiai ia??iaeo niieith o ?ineei ia?oiai ?ieo
aeeooy; aecia/eoe oiiae aaaaeaiiy a eoeueoo?o in vitro ca aeiiiiiaith
iioei?caoe?? oiia io?eiaiiy eaethnieo eoeueoo? c eenoiaeo,
n?i’yaeieueieo, aiiieioeeaaeo aenieaioia ? eaoa?aeueieo a?oiueie,
aea/eoe caeaaei?noue ii?oiaaiaco eaoa?aeueieo a?oiueie a eoeueoo?? a?ae
ni?aa?aeiioaiiy o?oiai?iii?a o iiaeeaieo na?aaeiaeuao; ?ic?iaeoe
?aeiiaiaeaoe?? ii caa?aaeaiith oa i?enei?aiith ?iciiiaeaiiy oe?iieo
aaiioei?a.

Iaoeiaa iiaecia iaea?aeaieo ?acoeueoao?a. Aeine?aei? ?ineeie M.
officinal?s ni?oic?ace?a e-36672, e-36673, e-36674, e-36675, e-36676,
e-36680 c eieaeoe?? Ana?in?enueeiai ?inoeoooo ?ineeiieoeoaa ?iai?
I.?.Aaa?eiaa a oiiaao oeaio?aeueii? oa i?aaeaiii-no?aeii? Oe?a?ie o
ia?oee ??e aeeooy ia ia?aoiaeyoue aei ianiaiai oea?o?iiy. Aoee
?ic?iaeai? oeyoe ?ioaineo?eaoe?? ?ia?o ni?yiiaaieo ia i?aeaeuaiiy
eiia??iino? noai?aiiy oa caa?aaeaiiy ni?o?a ?c aaaeaieie iciaeaie ca
?aooiie canoinoaaiiy aeiooaiiai ia??iaeo niieith ?ineei ia?oiai ?ieo
aeeooy. A ?acoeueoao? aea/aiiy ?icaeoeo ?ineei M. officinal?s ia?oiai
?ieo aeeooy ?iciiiaeaieo aaaaoaoeaii anoaiiaeaii, ui c-ca iaaei?icaeoeo
ceioth/eo i?aai?a oe? ?ineeie ia caeaoi? ia?aiineoe in?iiuei-ceiiaee
ia??iae oa ooai?thaaoe ian?iiy. Aeey i?enei?aiiai ia?aoiaeo ?ineei
ia?oiai ?ieo aeeooy, ui aoee ?iciiiaeai? aaaaoaoeaii, aei oea?o?iiy aoei
cai?iiiiiaaii canoinoaaiiy oioi?iaeoeoe??, ia i?ioyc? aeiooaiiai
ia??iaeo niieith. Aoee ?ic?iaeai? oiiae aaaaeaiiy M. officinal?s a
eoeueoo?o in vitro, oaeiae nenoaia ?iaeoeoe?? eaethniaaiaco ia
eenoeiaeo, n?i’yaeieueieo, aiiieio?eaaeo aenieaioao oa eaoa?aeueieo
a?oiueeao. Aeyaeaii caeaaei?noue i?ioean?a ii?oiaaiaco a eoeueoo??
eaoa?aeueieo a?oiueie a?ae ni?aa?aeiioaiiy o?oiai?iii?a o iiaeeaieo
na?aaeiaeuao.

I?aeoe/ia cia/aiiy io?eiaieo ?acoeueoao?a. Iaoa??aee aeena?oaoe??, aoee
aeei?enoai? ni?a?ia?oieeaie eaoaae?e aioai?ee oa aeieia?? ia
aenia?eiaioaeuei?e aac? Eoaainueeiai aea?aeaaiiai aa?a?iiai oi?aa?neoaoo
i?e io?eiaii? iiaiai ni?oo: Ao?eoi aeiaoee Eoaainueeee iaeieoia?eiiaee,
yeee cayaeaii o Aea?aeaaio eii?n?th ii ni?oiaei?iaoaaiith aeey caianaiiy
o ni?oiaee ?a?no? Oe?a?ie ia 1999 ??e. ?ic?iaeaiee eiiieaen iaoiae?a
aaaaoaoeaiiai ?iciiiaeaiiy oa oioi?iaeoeoe?? i?ae /an aeiooaiiai
ia??iaeo niieith iiaea aooe aeei?enoaiee i?e ?iciiiaeaii? oe?iieo
aaiioei?a ?ioeo eoeueoo? c aeecueeeie a?ieia?/ieie iniaeeainoyie. Ca
?acoeueoaoaie aeine?aeaeaiue ii ooai?aiith eaeonieo eoeueoo? c eenoyieo,
n?i’yaeieueieo, aiiieioeeaaeo aenieaio?a oa eaoa?aeueieo a?oiueie aoei
io?eiaii aeo?aeiee iaoa??ae aeey naeaeoe?? oa nei?i/aii o?eaae?noue
naeaeoe?eiiai i?ioeano M. officinal?s. E??i oiai, cai?iiiiiaai? iaoiaee
eoeueoeaoaaiiy oa ?aaaia?aoe?? ?ineei iiaeooue

aooe canoiniaai? aeey eeiioaaiiy oe?iieo aaiioei?a, naiieeiiaeueieo
aa??aio?a oa iooaioieo oi?i, a oaeiae aei?iuoaaiiy a?a?eaeieo ca?iaee?a.

Iniaenoee aianie caeiaoaa/a a ?acoeueoao? aeine?aeaeaiue, ui aeeeaaeai?
a aeena?oaoe?? oa ioae?eaoe?yo (naiino?eii aai a ni?aaaoi?noa?), iieyaa?
a o/ano? a ?ic?iaoe? i?ia?ai oa iaoiaeee aeine?aeaeaiue, i?aai?caoe?? e
i?iaaaeaii? aeine?aeaeaiiy a?ieia?/ieo iniaeeainoae aaaaoaoeaiiai
?iciiiaeaiiy M. officinal?s.

Ai?iaaoe?y ?acoeueoao?a aeena?oaoe??. ?acoeueoaoe aeena?oaoe?? aoee
aeeeaaeai? ia iaoeiaeo eiioa?aioe?yo Eoaainueeiai aea?aeaaiiai aa?a?iiai
oi?aa?neoaoo oa E??iaia?aaenueeiai aea?aeaaiiai iaaeaaia?/iiai
oi?aa?neoaoo a 1993-1999?.?.

Ioae?eaoe??: Ca ?acoeueoaoaie aeine?aeaeaiue iioae?eiaaii 7 iaoeiaeo
i?aoeue, c ieo 4 — o oaoiaeo aeaeaiiyo, c yeeo 3 — naiino?eii, 1 — o
ni?aaaoi?noa?.

No?oeoo?a oa ianya aeena?oaoe??: Aeena?oaoe?y neeaaea?oueny ?c anooio, 3
?icae?e?a oa aeniiae?a. ?iaioa aeeeaaeaia ia 110 noi??ieao oaenoo,
aeeth/a? 8 oaaeeoeue, 10 ?enoie?a. Nienie aeei?enoaieo aeaea?ae iaoeiai?
e?oa?aoo?e i?noeoue 196 iaeiaioaaiue, o oiio /ene? ca?oa?aeieo aaoi??a —
36.

?ICAeIE 1. IAEssAe EIOA?AOO?E II A?IEIA?*IEI INIAEEAINOssI IALILOTUS
OFFICINALIS (L.)PALL. OA AAAAOAOEAIIIO ?ICIIIAEAIITH ?INEEI

Iaaaaeaii iaeyae e?oa?aoo?e c ieoaiue ii?oi-o?c?ieia?/ieo iniaeeainoae
oa iioiaaiaco I. officinalis .

?icaeyaeathoueny iaoiaee aaaaoaoeaiiai ?iciiiaeaiiy ?ineei, ye?
iioe?aii canoiniaoaaoe aeey caa?aaeaiiy oe?iieo aaiioeiieo iciae.

?ICAe?E 2. IAOA??AEE OA IAOIAeEEE AeINE?AeAEAIUe

Aeine?aeaeaiiy i?iaiaeeeenue ia eaoaae?? aioai?ee oa aeieia??
Eoaainueeiai aea?aeaaiiai aa?a?iiai oi?aa?neoaoo o 1991-1994 ?ieao oa ia
eaoaae?? i?e?iaeie/i–iaoaiaoe/ieo aeenoeeie?i E??iaia?aaenueeiai
aea?aeaaiiai iaaeaaia?/iiai oi?aa?neoaoo o 1994-1999 ?ieao. Ia`?eoaie
aeine?aeaeaiue aoee ni?oic?acee ao?eoio aeiaoiai iaeieoia?eiiaiai
e-36672, e-36673, e-36674, e-36675, e-36676, e-36680, io?eiai? c
eieaeoe?eiiai oiiaeo Ana?in?enueeiai ?inoeoooo ?ineeiieoeoaa. Aoei
anoaiiaeaii iniaeeaino? naciiiiai ?icaeoeo aeine?aeieo ni?oic?ace?a o
eiie?aoieo oiiaao i?noey i?iaaaeaiiy aeine?ae?a.

Aaaaoaoeaia ?iciiiaeaiiy aea/aeinue oeyoii aenia?eiaio?a ii oei?ai?iith
caeaieo aeeaoe?a oa aaaaeaiith aenieaio?a M. officinalis a eoeueoo?o in
vitro.

Aea/aiiy a?ieia?/ieo iniaeeainoae M. officinalis i?e aaaaoaoeaiiio
?iciiiaeaii? i?iaiaeeee ia ?ineeiao ni?oic?aceo e-36672, ine?eueee i?e
i?iaaaeaii? naeaeoe?eieo ?ia?o ni?a?ia?oieee eaoaae?e aioai?ee oa
aeieia?? Eoaainueeiai aea?aeaaiiai aa?a?iiai oi?aa?neoaoo aoee iaea?eueo
caoe?eaaeai? naia eiai ainiiaea?nueei-oe?iieie iciaeaie. Ioe?ieo ai?noo
eoia?eio i?iaiaeeee ca iaoiaeii o?iiaoia?ao??; iiooaei?noue ?icaeiaii?
ei?aiaai? nenoaie, yea ooai?eeanue ia aeeaoeyo, aea/aee ca iaoiaeii
Oo?aoeueei? (1968). ?icaeoie ii?oio?c?ieia?/ieo i?enoinoaaiue aei
ia?aianaiiy ceiiaeo oiia o ?ineei M. officinalis ia?oiai ?ieo aeeooy oa
?o ?aaaia?aio?a ioe?ithaaee ca aeiiiiiaith iaoiaeeee, ?ic?iaeaii? ia
eaoaae?? aioai?ee oa aeieia?? Eoaainueeiai aea?aeaaiiai aa?a?iiai
oi?aa?neoaoo. Aea/aiiy ii?oieia?/ieo iniaeeainoae ei?aiaai? nenoaie i?e
oei??iaii? aeeaoe?a ia i?neo i?iaiaeeee ca iaoiaeii ?ioi?no?iaa (1965).
?iaioe ii aaaaeaiith aenieaio?a M. officinalis a eoeueoo?o in vitro
i?iaiaeeee o eaai?aoi??yo eoeueoo?e ?cieueiaaieo oeaiei o anaioe/ieo
oiiaao ca caaaeueiii?eeiyoith iaoiaeeeith ?iaioe c eoeueoo?ith oeaiei.

?i?oe?aoe?th ia?aoiaeo ?ineei M. officinalis ia?oiai ?ieo aeeooy aei
oea?o?iiy i?iaiaeeee ca iaoiaeii oioi?iaeoeoe??.

Aeine?aee caeeaaeaee ca?aeii c caaaeueiii?eeiyoeie iaoiaeeeaie
i?iaaaeaiiy a?ieia?/ieo aenia?eiaio?a (Aeini?oia, 1979). ?ic-i?uaiiy
aa??aio?a aeine?aeo i?iaiaeeee oeyoii ?aiaeii?caoe??. Eiaeai aeine?ae
iaa /ioe?ee?aoio iiaoi?thaai?noue. E?euee?ni? iieacieee ii?aoeueiaoaaee
ca aeiiiiiaith aa??aoe?eii-noaoenoe/ieo iaoiae?a. A??ia?aei?noue
aecia/aee ca e?eoa???i nooo?aino? Nouethaeaioa oa i.?.?., ye? io?eioaaee
i?e i?iaaaeaii? aeenia?niiai oa ei?aeyoe?eiiai aiae?c?a (Eaeei, 1990).
On? iaoaiaoe/i? ?ic?aooiee aeeiioaaeenue ca aeiiiiiaith i?ia?ai
noaoenoe/iiai aiae?co Arm statistic oa Microsoft Excel` 97 ia
eiii’thoa?? Pentium II-586.

?ICAeIE 3. AIIEIAI*II INIAEEAINOI MELILOTUS OFFICINALIS (L.)PALL. I?E
AAAAOAOEAIIIO ?ICIIIAEAIII

3.1 Iniaeeaino? aaaaoaoeaiiai ?iciiiaeaiiy Melilotus officinalis
(L.)Pall caeaieie aeeaoeyie c ?ineei ia?oiai oa ae?oaiai ?ie?a aeeooy

3.1.1 Iniaeeaino? ?icaeoeo aeine?aeiiai ni?oic?aceo a oiiaao
oeaio?aeueii? oa i?aaeaiii-no?aeii? Oe?a?ie.

A i?ioean? aea/aiiy iniaeeainoae naciiiiai ?icaeoeo aeine?aeieo
ni?oic?ace?a a?aeiia?aeii aei aaia?ao?/iiai iieiaeaiiy oa
aa?iee?iaoe/ieo oiia i?noey i?iaaaeaiiy aeine?ae?a aoei anoaiiaeaii, ui
ia a?aei?io a?ae iaaaaeaieo a eaoaeic? A?? aeaieo, aeine?aei? ?ineeie o
ia?oee ??e aeeooy aei ianiaiai oea?o?iiy ia ia?aoiaeeee. Aecia/ai?
?ica?aeiino? iia`ycai? c ??ciith o?eaae?noth na?oeiaiai aeiy a
Naieo-Iaoa?ao?c? (i?noeaciaoiaeaeaiiy A??) oa ia oa?eoi??? oeaio?aeueii?
oa i?aaeaiii-no?aeii? Oe?a?ie (i?noea i?iaaaeaiiy aeine?ae?a).

3.1.2.Iniaeeaino? oei??iaiiy caeaieo aeeaoe?a Melilotus officinalis
(L.)Pall c ?ineei ia?oiai oa ae?oaiai ?ieia aeeooy.

A oiae? aeine?aeaeaiue aoei ?ic?iaeaii iiaee i?aeo?ae aei ?iciiiaeaiiy
aeai??/ieo enaiiaaiieo ei?iiaeo ?ineei iii??ii? ciie ia iniia?
canoinoaaiiy aaaaoaoeaiiai ?iciiiaeaiiy oa oioi?iaeoeoe?? aei
?aaaia?aio?a c ?ineei ia?oiai ?ieo aeeooy i?ioyaii in?iiuei-ceiiaiai
ia??iaeo, ia iniia? /iai nei?i/o?oueny ia??iae ca?eueoaiiy a iiioeyoe?yo
e?eueeino? ?ineei c oe?iieie aaiioeiieie iciaeaie (ia 1 ??e).
Aeine?aeaeaiiy iniaeeainoae aaaaoaoeaiiai ?iciiiaeaiiy M. officinalis
caeaieie aeeaoeyie c ?ineei ia?oiai oa ae?oaiai ?ie?a aeeooy iieyaaei o
aecia/aii? iniaeeainoae ?aaaia?aoe?? ei?ai?a. Ei?aiaooai?aiiy ia
aeeaoeyo ii/eiaeinue o ia?oee oeaeaeaiue a?ae /ano eeiioaaiiy oa o?eaaei
aeecueei 5 oeaei?a. Aoaeoeai?noue oei??iaiiy aeeaoe?a M. officinalis
ioe?ithaaee ye a?aeiioaiiy e?eueeino? aeeaoe?a, ui ooai?eee ei?ai?, aei
?o caaaeueii? e?eueeino?. Aeenia?niee aiae?c ?acoeueoao?a aeine?aeo
na?ae/ea i?i ia?aaeuaiiy noaiaea?oiiai (Fst=2,0;2,2;3,3) e?eoa??th
nooo?aino? iaae oaeoe/iei (F=1,3). Oiio ie aaaaea?ii, ui a?e aeeooy
?ineei ia iiiaio eeiioaaiiy iaei aieeaa? ia aoaeoeai?noue oei??iaiiy.
Ne?ae a?aecia/eoe, ui o caeaieo aeeaoe?a c ?ineei M. officinalis
ia?oiai ?ieo aeeooy ei?aiaooai?aiiy i?ioiaeeei oaeaeoa oa c iaioith
?ioaineai?noth, i?ae o aeeaoe?a c ?ineei ae?oaiai ?ieo aeeooy ( oaae.
3.1).

Oaaeeoey 3.1

Caeaaei?noue aeeiai?/ieo oa a?iiao?e/ieo oa?aeoa?enoee ei?aiaooai?aiiy
ia caeaieo aeeaoeyo I. officinalis a?ae a?eo ?ineei ia iiiaio eeiioaaiiy
(a na?aaeiueiio ca 4 ?iee).

Iieaciee??e aeeooy ?ineeie ia iiiaio eeiioaaiiyA?aeoeeaiiyi.i.?.Ia?oee
(eiio?ieue)Ae?oaee

± aei eiio?ieth ± aei eiio?iethNa?aaei? ca iiaoi?iinoyiea %1.Ia??iae
a?ae ii-/aoeo aeine?aeo aei ii/aoeo ei?aia-ooai?aiiy (aei?a)

14.1±3.6

17.1±2.9

+3.0*

+21.3

2.92.Caaaeueia o?eaae?noue ?icaeoeo ei?ai?a ia aeeaoeyo (aei?a)

20.0±3.6

23.9±2.7

-3.9*

-19.5

3.73.O?eaae?noue ?inoo ei?ai?a ia aeeaoeyo (aei?a)

6.6±3.6

6.4±4.0

-0.2

-3.0

1.94.E?euee?noue ei?a-i?a ia aeeaoeyo (oo.)

7.1±4.6

11.1±3.7

-4.0*

-57.1

3.25. I?e??no noaa-eiai? /anoeie aeeaoey i?ioyaii oei??iaiiy (ni)

4.2±2.0

4.7±2.2

-0.7

-1.1

2.6I?eiioea. *?icieoey i?ae aa?iaioia aeineiaeo noaoenoe/ii ai?iaiaeia
ca e?eoa?i?i Nouethaeaioa i?e ?iaii cia/ouinoi p<0.05. Caaaeueia e?euee?noue ei?ai?a, ui ooai?thaaeenue ia aeeaoeyo c ?ineei ae?oaiai ?ieo aeeooy, aoea ia 57.1% a?eueoith, i?ae ia aeeaoeyo c ?ineei ia?oiai ?ieo aeeooy. Aeenia?niee aiae?c ?acoeueoao?a aeine?aeo na?ae/ea i?i ia?aaeuaiiy oaeoe/iiai e?eoa??th nooo?aino? iaae noaiaea?oiei (Fst=2,0;2,2;3,3) i?e ii??aiyii? aa??aio?a aeine?aeo: ca ia??iaeii ei?aiaooai?aiiy ia aeeaoeyo (Fo=4.5), ca e?euee?noth ei?ai?a (Fo=46.3) oa caaaeueiith o?eaae?noth ei?aiaooai?aiiy (Fo=4.1). Oaeei /eiii, ??e aeeooy ?ineeie ye oaeoi? aeine?aeo iaa aeniei a??ia?aeio aieeaia?noue (? <0,05) ia cacia/ai? iieacieee. Ei?aeyoe?eiee aiae?c ?acoeueoao?a aeine?aeo na?ae/ea i?i iayai?noue a??ia?aeieo ca’yce?a i?ae a?iiao?e/ieie oa aeeiai?/ieie oa?aeoa?enoeeaie ei?aiaooai?aiiy. Ia iniia? oeueiai a iaio aa??aioao aeine?aeo aoei aecia/aii a??ia?aeio iiceoeaio caeaaei?noue ia??iaeo aei ii/aoeo ei?aiaooai?aiiy a?ae ia??iaeo aei ii/aoeo eaethniooai?aiiy (r1=+0.91; r2=+0,86), iaaaoeaio caeaaei?noue e?eueeino? ei?ai?a ia aeeaoeyo a?ae ia??iae?a aei ii/aoeo eaethniooai?aiiy (r1=-0,58; r2=-0,49) oa ei?aiaooai?aiiy (r1=-0,56; r2=-0,44), a oaeiae iiceoeaio caeaaei?noue oaeaeeino? ?inoo ei?ai?a a?ae ia??iae?a aei ii/aoeo eaethniooai?aiiy (r1=+0,42; r2=+0,35) oa ei?aiaooai?aiiy (r1=+0.51; r2=+0,46). Oaeei /eiii, ca?eueoaiiy e?eueeino? ei?ai?a o aeeaoe?a c ?ineei ae?oaiai ?ieo aeeooy ii??aiyii c aeeaoeyie c ?ineei ia?oiai ?ieo aeeooy aoea coiiaeaia ca?eueoaiiyi o?eaaeino? ia??iae?a aei ii/aoeo i?ioean?a ei?aiaooai?aiiy. ?aaaia?aoe?y ei?ai?a ia aeeaoeyo c ?ineei M. officinalis i?ioiaeeea ca aeaiia oeiaie: /a?ac eaethn ia iinoei c?ico oa /a?ac aioo?ioii oeaieie ia noaae?. Ne?ae a?aecia/eoe, ui ?aaaia?aoe?y ei?ai?a /a?ac aioo??oi? oeaieie aeyaeeany iaio aoaeoeaiith, i?ae /a?ac eaethn o aeeaoe?a c ?ineei ye ia?oiai, oae ? ae?oaiai ?ie?a aeeooy (a?aeiia?aeii ia 53% oa 45%). Eiao?oe??io aaaaoaoeaii? ?ai?iaeoeoe??, io?eiaiee ia iniia? e?eueeino? aeeaoe?a, ui aeaee ei?ai?, neeaaeaa 1:65.1±11.2. I?e o?aooaaii? ian?iii? i?iaeoeoeaiino? c eeiio iaeiiai noaaea io?eioaaee a na?aaeiueiio 65.1±5.6a ian?iiy. Oaeei /eiii, eiao?oe??io ?iciiiaeaiiy noaiiaea 1:29,6±2.6. 3.1.3Aiae?c ii?oi-o?c?ieia?/ieo i?enoinoaaiue aei ia?aianaiiy iani?eyoeeaeo oiia in?iiuei-ceiiaiai ia??iaeo o ?ineei Melilotus officinalis (L.)Pall ia?oiai ?ieo aeeooy, ?icaeiaieo c ian?iiy oa ?iciiiaeaieo aaaaoaoeaiiai. A oiae? aeine?aeaeaiiy iniaeeainoae ?icaeoeo ii?oi-o?c?ieia?/ieo i?enoinoaaiue aei ia?aianaiiy iani?eyoeeaeo oiia in?iiuei-ceiiaiai ia??iaeo o ?ineei M. officinalis ia?oiai ?ieo aeeooy, ye? aoee ?iciiiaeai? aaaaoaoeaii, anoaiiaeaii, ui aeaoi?iaoe?y i?ia?aeieo aeaiaio?a ei?aiy ia a?aeaoaaeanue c i?e/eie iacia/iiai iaeiie/aiiy e?ioiaeth a caianath/?e ia?aio?i?, oiio, noaaeiaa /anoeia, yea iana iacooi? a?oiueee, caeeoaeanue ia iiaa?oi? a?oioo. Aiae?c aoaeiae iacooieo a?oiueie ieaeiueiai i?aeaocey na?ae/ea i?i a?aenooi?noue ci?i, ye? iaee a caaacia/eoe ?o caoeno a?ae iani?eyoeeaeo oiia in?iiuei-ceiiaiai ia??iaeo. Oaeei /eiii, iaea?aeaoe ian?iiy c ?ineei M. officinalis, ye? aoee ?iciiiaeai? aaaaoaoeaii, o ia?oee ??e aeeooy a iieueiaeo oiiaao aeyaeeiny iaiiaeeeaei. 3.1.4. I?enei?aiiy ia?aoiaeo aei oea?o?iiy ?ineei Melilotus officinalis (L.)Pall ia?oiai ?ieo aeeooy, ?icaeiaieo c ian?iiy oa ?iciiiaeaieo aaaaoaoeaiiai i?e canoinoaaii? oioi?iaeoeoe??. A oiae? aeine?aeaeaiue aoei anoaiiaeaii, ui o ?ac? ina?oeaiiy ?ineei M. officinalis ia?oiai ?ieo aeeooy a ?aaeei? (18 aiaeei ia aeiao) oea?o?iiy ii/eiaeinue a na?aaeiueiio /a?ac 38.0 ± 5.0 aei?a. ?ineeie ia?oiai ?ieo aeeooy, ooai?ai? c ian?iiy oa ?iciiiaeai? aaaaoaoeaii, ye? ia?aoiaeeee aei oea?o?iiy i?ae aieeaii oioi?iaeoeoe??, ca ia??iaeii oa ?ioaineai?noth cacia/aiiai i?ioeano a??ia?aeii ia a?ae??ciyeenue. I?ae /an niinoa?aaeaiue ca aeine?aeieie ?ineeiaie M. officinalis oeyoii ei?aeyoe?eiiai aiae?co aoea aecia/aia caeaaei?noue e?eueeino? nooea?oue ia ?ineei? a?ae aeo?aeii? aenioe ?ineeie ia iiiaio ii/aoeo oioi?iaeoeoe?? (r=+0.73). Eiao?oe??io ?iciiiaeaiiy ?ineei M. officinalis ia?oiai ?ieo aeeooy i?e canoinoaaii? aaaaoaoeaiiai ?iciiiaeaiiy oa oioi?iaeoeoe?? noaiiaea 1:15.9±1.2. 3.2. Canoinoaaiiy o?c?ieia?/ii-aeoeaieo ?a/iaei a?oie aoenei?a aeey noeioeyoe?? oei??iaiiy caeaieo aeeaoe?a Melilotus officinalis (L.)Pall C iaoith ?ioaineo?eaoe?? aaaaoaoeaiiai ?iciiiaeaiiy canoiniaoaaee IIE, ?IE o eiioeaio?aoe?yo a?ae 5 aei 50 ia/e. o ??cieo ni?aa?aeiioaiiyo. Ca iieacieeaie aoaeoeaiino? oei??iaiiy aeeaoe?a, caeaaeii a?ae ia??iaeo eoeueoeaoaaiiy oa eiioeaio?aoe?? o?oiai?iii?a, aeenia?niee aiae?c iieacaa ia?aaeuaiiy oaeoe/iiai e?eoa??th nooo?aino? (F=7.2) iaae noaiaea?oiei (Fst 2.0,2.2,3.3). Oaeei /eiii, ia??iae eoeueoeaoaaiiy oa eiioeaio?aoe?? o?oiai?iii?a iaee a??ia?aeiee aieea ia e?euee?noue oei??iaieo aeeaoe?a M. officinalis. Ne?ae a?aecia/eoe, ui iaeneiaeueio e?euee?noue aeeaoe?a, ia yeeo ooai?eeenue ei?ai? i?ioyaii oeaeiy eoeueoeaoaaiiy ( ia 22.3%, ii??aiyiii aei eiio?ieth), a?aei?/aii o aeeaoe?a, ia?iaeaieo ?ic/eiii IIE (20ia/e), ia ae?oaiio oeaei? a?ae ii/aoeo aeine?aeo. Ca?eueoaiiy e?eueeino? oei??iaieo aeeaoe?a M. officinalis i?e canoinoaaii? IIE oa ?IE o eiioeaio?aoe?yo a?ae 5 aei 50 ia/e aeyaeaii ia aoei. IIE o eiioeaio?aoe?? 20ia/e - caaacia/oaaea ca?eueoaiiy e?eueeino? ei?ai?a (o 2,2 ?ace), oiaoi ni?eyea ?icaeoeo a?eueo iiooaeii? ei?aiaai? nenoaie. Ia i?neo e?euee?noue ei?aiaaeo ca/aoe?a ia aeeaoeyo aoea iaioith, i?ae ia aiae? (ia 59,9%). Canoinoaaiiy IIE i?ecaiaeeei aei aaeueioaaiiy ?icaeoeo noaaeiai? /anoeie ?aaaia?aio?a (ia 72,4%) oa ca?eueoaiith ia??iaeo ia?aoiaeo aei oea?o?iiy (ia 26,6% ). I?ae aieeaii IIE caeaaei?noue ?ioaineaiino? i?ioean?a ooai?aiiy ei?ai?a ia caeaieo aeeaoeyo M. officinalis a?ae o?eaaeino? ia??iae?a aei ii/aoeo eaethniooai?aiiy oa ei?aiaooai?aiiy iaei i?inoaaeoaaeanue. A ?acoeueoao? i?iaaaeaiieo aeine?aeaeaiue aoei anoaiiaeaii, ui noeioethth/iai aoaeoo ia aoaeoeai?noue oei??ithaaiiy aeeaoe?a M. officinalis aoeneie ia aeyaeee, aea ni?eyee ?icaeoeo a?eueo iiooaeii? ei?aiaai? nenoaie. 3.3.Iniaeeaino? ooai?aiiy ei?ai?a ia caeaieo aeeaoeyo aenieieoia?eiiaeo oa iecueeieoia?eiiaeo oi?i Melilotus officinalis (L.)Pall A ?acoeueoao? i?iaaaeaieo aeine?ae?a aoei anoaiiaeaii, ui iecueeieoia?eiia? oa aenieieoia?eiia? oi?ie M. officinalis ca aoaeoeai?noth ?aaaia?aoe?? ei?ai?a ia caeaieo aeeaoeyo ( 56.7±11.1% oa 63.3%) a??ia?aeii ia a?ae??ciyeenue. ?ic/ei nieae ca i?iienii Eiiio i?eai?/oaaa ??aaiue oei??iaiiy aeeaoe?a M. officinalis (ia 23,3-30%). I?e eoeueoeaoaaii? aeeaoe?a iecueeieoia?eiiaeo oi?i M. officinalis ia nieueiaiio ?ic/ei? Eiiio ii/aoie ei?aiaooai?aiiy i?enei?thaaany (ia 15%), a o aenieieoia?eiiaeo oi?i cao?eioaaany (ia 26,7%). 3.4.Oiiae aaaaeaiiy ?ineei Melilotus officinalis (L.)Pall a eoeueoo?o in vitro I?e iaiao?aeiino? oaeaeeiai ?iciiiaeaiiy oe?iieo aaiioei?a M.officinalis ?iiae? aeioe?eueii canoiniaoaaoe iaoiaee i?e?i-eeiioaaiiy. I?enooi?noue o?oiai?iii?a o iiaeeaiiio na?aaeiaeu? coiiaeth? oa?aeoa? ii?oiaaiaco ia eaoa?aeueieo a?oiueeao M. officinalis. Oae, i?enooi?noue AAI o eiioeaio?aoe?? 1.0 ia/e ni?ey? iaaiiiaaiaco, IIE o eiioeaio?aoe?? 1.0 ia/e ni?ey? ?eciaaiaco, a aeei?enoaiiy iaio o?oiai?iii?a o eiioeaio?aoe?? 0.5 ia/e ni?ey? eaethniaaiaco. Oei aenieaioo coiiaeth? iniaeeaino? i?ie?oa?aoe?? eaethn?a. I?e i?ie?oa?aoe?? eaethn?a ia? i?noea aca?iiae?y aieeao iiaeeaiiai na?aaeiaeua oa oeio aenieaioo. I?e eoeueoeaoaaii? aenieaio?a M. officinalis iiaeeaia na?aaeiaeua IN ia? ia?aaaao ca ?ioaineai?noth i?ie?oa?aoe?? eaethn?a ii??aiyii c A5.9 . AENIIAEE 1. Eiiieaen iaoiae?a, ?ic?iaeaiee iaie ia iniia? aea/aiiy a?ieia?/ieo iniaeeainoae ?icaeoeo M. officinalis oa aaaaoaoeaiiai ?iciiiaeaiiy M. officinalis, ? ni?yiiaaiee ia i?enei?aiiy ca?eueoaiiy a iiioeyoe?yo e?eueeino? ?ineei c oe?iieie aaiioeiieie iciaeaie, ia? aaeeea i?aeoe/ia cia/aiiy. ?ic?iaeai? iaoiaee aeine?aeaeaiue aoee aeei?enoai? ni?a?ia?oieeaie eaoaae?e aioai?ee oa aeieia?? Eoaainoeiai aea?aeaaiiai aa?a?iiai oi?aa?neoaoo a ?iaioao ii noai?aiith iiaiai ni?oo: Ao?eoi aeiaoee Eoaainueeee iaeieoia?eiiaee. 2. I?iaaaeai? oaiieia?/i? niinoa?aaeaiy ca iniaeeainoyie ?icaeoeo ni?oic?ace?a e-36672, e-36673, e-36674, e-36675, e-36676, e-36680 Ao?eoio aeiaoiai (ye? iaeaaeaee aei aia?eeainueei? aeieia?/ii? a?oie oa aia?eeainueeiai aeioeio), io?eiaieo c eieaeoe?eiiai oiiaeo A??, aeicaieeee anoaiiaeoe, ui aa?iee?iaoe/i? oiiae oeaio?aeueii? oa i?aaeaiii-no?aeii? Oe?a?ie a oe?eiio ni?eyoeea? aeey ?inoo ? ?icaeoeo aeine?aeieo ?ineei, aea, ia a?aei?io a?ae aeaieo eaoaeiao A??, ianiaiai oea?o?iiy o ??e iin?ao o ieo ia a?aeaoaa?oueny. 3. Aeiaaaeaii, ui a?e ?ineeie ia iiiaio eeiioaaiiy ia iaa (56,7-63.3%) a??ia?aeiiai aieeao ia aoaeoeai?noue ?aaaia?aoe?? ei?ai?a o caeaieo aeeaoe?a M. officinalis, aea coiiaethaaa aeeiai?eo, oa?aeoa? oa iiooaei?noue ei?aiaooai?aiiy. Eeiioaaiiy ?ineei M. officinalis ia?oiai ?ieo aeeooy aeioe?eueii i?iaiaeeoe iai?ee?ioe? /a?aiy, a ?ineei ae?oaiai ?ieo aeeooy - iai?ee?ioe? o?aaiy. 4. Anoaiiaeaii, ui ?aaaia?aioe ?ineei M. officinalis ia?oiai ?ieo aeeooy ia caeaoi? ia?aiineoe iani?eyoeea? oiiae in?iiuei-ceiiaiai ia??iaeo oa aeaaaoe a?ae?inoaiiy aaaaoaoeaii? iane iaaani? o ca’yceo c iaaei?icaeoeii ceioth/eo i?aai?a. Oiio io?eiaoe ian?iiy c ieo iiaeeeai eeoa o ?ac? canoinoaaiiy aeiaeaoeiaeo caoiae?a ia iniia? oioi?iaeoeoe?? o ?ic?iaeaieo iaie oiiaao oioi?aaeeio (18-24 aiaeeie ia aeiao). 5. Anoaiiaeaii nooi?iue caeaaeiino? iiooaeiino? ?icaeiaii? ei?aiaai? nenoaie, yea ooai?thaaeanue ia caeaieo aeeaoeyo M. officinalis, oa oaeaeeino? ?? ?icaeoeo a?ae aieeao o?c?ieia?/ii-aeoeaieo ?a/iaei a?oie aoenei?a. 6. Aeine?aeaeaii, ui o iecueeieoia?eiiaeo oa aenieieoia?eiiaeo oi?i M. officinalis ei?aiaooai?aiiy caeaieo aeeaoe?a i?ioiaeeoue iaeaea ?aeaioe/ii. 7. Canoinoaaiiy aei caeaieo aeeaoe?a M. officinalis ?ic/eio IIE (o eiioeaio?aoe?? 20ia/e oa c aeniiceoe??th 5 aiaeei) ni?ey? ?icaeoeo a?eueo iiooaeii? ei?aiaai? nenoaie, aea ia aeyaey? aieeao ia aoaeoeai?noue oei??iaiiy aeeaoe?a. Ia i?neo IIE aieeaa? ia ca?eueoaiiy e?eueeino? ei?aiaaeo ca/aoe?a (ia 17.9%). 8. Ca?eueoaiiy ai?noo iiaeeaieo ?a/iaei o na?aaeiaeu? (?ic/ei Eiiia, i?nie) cieaeo? aoaeoeai?noue oei??iaiiy caeaieo aeeaoe?a M. officinalis. 9. I?e aaaaeaii? ?ineei M. officinalis a eoeueoo?o in vitro aoei anoaiiaeaii, ui i?enooi?noue AAI o eiioeaio?aoe?yo a?ae 0.5 aei 1.0 ia/e ni?eyea iaaiiiooai?aiith ia eaoa?aeueieo a?oiueeao, IIE o eiioeaio?aoe?yo a?ae 0.5 aei 1.0 ia/e- ?eciaaiaco, a iaio o?oiai?iii?a iaeii/anii o eiioeaio?aoe?? a?ae 0.1 aei 0.5 ia/e - eaethniaaiaco. Oei aenieaioa oa neeaae iiaeeaiiai na?aaeiaeua coiiaeththoue iniaeeaino? i?ie?oa?aoe?? eaethn?a. A oiae? aeine?aeaeaiue aoei aecia/aii, ui iiaeeaia na?aaeiaeua IS, ii??aiyii c A5.9, iineeth? ?ioaineai?noue eaethniaaiaco. Ioae?eaoe?? 1.Oa?/aiei A.?., Ei?i?/ia I.A. Aaaaoaoeaia ?iciiiaeaiiy ao?eoio. // Noaiiaa caiea?ianoai.- 1995.- ? 29. - N.78-80. 2.Oa?/aiei A.?. Iniaeeaino? aaaaoaoeaiiai ?iciiiaeaiiy ao?eoio aeeaoeyie c ?ineei ia?oiai oa ae?oaiai ?ie?a ?icaeoeo // Noaiiaa caiea?ianoai.- 1996.- ? 30.-N.58-60. 3.Oa?/aiei A.?. A?ieia?/i? iniaeeaino? ? ?aaaia?aoe?y ei?ai?a ia aeeaoeyo aeniei- oa iecueeieoia?eiiaeo oi?i Melilotus officinalis (L.)Pall. // Ca. iaoe. i?aoeue EAeAO.- Eoaainuee.- 1999.-?4(11).-N.26-28. 4.Oa?/aiei A.?. Canoinoaaiiy oioiia??iaee/ii? ?iaeoeoe?? oea?o?iiy aeey ?ioaineo?eaoe?? aaaaoaoeaiiai ?iciiiaeaiiy oe?iieo aaiioei?a Melilotus officinalis (L.)Pall. // Ca. iaoe. i?aoeue EAeAO.- Eoaainuee.- 1999.-?4(11).-N.23-25. 5.Oa?/aiei A.A. E aii?ino i iee?ieeiiaeueiii ?aciiiaeaiee aeiiieea ec iacooiuo ii/ae.// Iaoa?eaeu io/aoiie iao/ii-oaoie/aneie eiioa?aioeee nio?oaeieeia ENOE.- Eoaainuee.- 1995.-N.48. 6.Oa?/aiei A.A. Iniaaiiinoe ?aaaia?aoeee ei?aiae o iaeenoaaiiuo /a?aieia aeiiieea ia ianea.// Iaoa?eaeu io/aoiie iao/ii-oaoie/aneie eiioa?aioeee nio?oaeieeia ENOE.- Eoaainuee.- 1995. -N.49. 7.Oa?/aiei A.A. Iniaaiiinoe aaaaoaoeaiiai ?iciiiaeaiey aeiiieea /a?aieaie n ?anoaiee ?aciuo eao aeecie // Iaoa?eaeu io/aoiie iao/ii-oaoie/aneie eiioa?aioeee nio?oaeieeia ENOE -Eoaainuee.- 1995. -N. 43. AIIOAOe?ss Oa?/aiei A. ?. A?ieia?/i? iniaeeaino? Melilotus officinal?s (L)Pall. i?e aaaaoaoeaiiio ?iciiiaeaii?.- ?oeiien. Aeena?oaoeiy ia caeiaoooy iaoeiaiai nooiaiy eaiaeeaeaoa aiieia?/ieo iaoe ca niaoeiaeueiinoth 03.00.05 - aioai?ea. Oeaio?aeueiee aioai?/iee naae ?i. I.I.A?eoea IAI Oe?a?ie, Ee?a, 1999. Aeena?oaoeiy i?enay/aia ?ic?iaeai iaoiaeia aaaaoaoeaiiai ?iciiiaeaiiy ?ineei ?icieo ?ieia aeeooy caiaeii ?o aiieiai/ieo iniaeeainoae. ?ic?iaeaii iaoiaeeeo i?enei?aiiai io?eiaiiy naeaeoeieiiai iaoa?iaeo oa oeyoe io?eiaiiy ianiiiy c ?aaaia?aioia ?ineei ia?oiai ?ieo ?icaeoeo, nenoaio io?eiaiiy eaethnieo eoeueoo? eenoeiaeo, nii’yaeieueieo oa aiiieioeeaaeo aenieaioia, a oaeiae eaoa?aeueieo a?oiueie. Ia iniiai ?aaoeyoei? aiinoo oioiai?iiiia a iiaeeaiiio n?aaeiaeui ciaeaeaii oeyoe iiaeoeoei? ii?oiaaiaco ia eaoa?aeueieo a?oiueeao ao?eoio. Cai?iiiiiaaii iaoiaeeee aeathoue iiaeeeainoue io?eiaoe aeoiaeiee iaoa?iae aeey naeaeoei? oa i?enei?eoe naeaeoeieiee i?ioean. Ii?aoethaaiiy iaoiaeia aaaaoaoeaiiai ?iciiiaeaiiy oa ?aeiiaiaeaoei? aei ?o canoinoaaiiy i?iaiaeeee, aeoiaey/e c aiieiai/ieo iniaeeainoae M. officinalis . Iniiaii ?acoeueoaoe ?iaioe ciaeoee i?aeoe/ia ai?iaaaeaeaiiy iiae /an ?iaio c aeaaaeaiiy iiaiai ni?oo: Ao?eoi aeiaoee Eoaainueeee iaeieoia?eiiaee. Eeth/ia? neiaa: aaaaoaoeaia ?iciiiaeaiiy, eeiioaaiiiy, aeeaoe?, iiaeeaia na?aaeiaeua, o?oiai?iiie, ei?aiaooai?aiiy, eaethn, eaoa?aeuei? a?oiueee, aenieaioe, ?aaaia?aoeiy. AIIIOAOeEss Oa?/aiei A. A. Aeieiae/aneea iniaaiiinoe Melilotus officinalis (L.)Pall. i?e aaaaoaoeaiii ?aciiiaeaiee ?oeiienue. Aeenna?oaoeey ia nieneaiea o/?iie noaiaie eaiaeeaeaoa aeieiae/aneeo iaoe ii niaoeeaeueiinoe 03.00.05 - aioaieea.- Oeaio?aeueiue aioaie/aneee naae ei. I.I.A?eoei IAI Oe?aeiu, Eeaa, 1999. Aeenna?oaoeey iinayuaia eco/aieth aeieiae/aneeo iniaaiiinoae e ?ac?aaioea iaoiaeia aaaaoaoeaiiai ?iciiiaeaiiy M. officinal?s aeey onei?aiey ianniaiai oaaee/aiey a iiioeyoeeyo naeaeoeeiiiuo oi?i n oeaiiuie aaiioeie/iuie i?eciaeaie e aeaeueiaeoaai eniieueciaaiey eo a naeaeoeee. A ?acoeueoaoa eco/aiey iniaaiiinoae ?acaeoey aaaaoaoeaii-?aciiiaeaiiuo ?anoaiee, iieo/aiiuo n eeiiia, ioia?aiiuo a ia?aue e aoi?ie aiae aeecie M. officinalis, onoaiiaeaii, /oi ec-ca iaaei?acaeoey ceiothueo i?aaiia ?aaaia?aiou n eeiiia ?anoaiee ia?aiai aiaea aeecie ia niiniaiu ia?aiineoue inaiia-ceiiee ia?eiae a anoanoaaiiuo oneiaeyo, neaaeiaaoaeueii, iieo/aiea io ieo naiyi noaiiaeoueny iaaiciiaeiui. Oaeei ia?acii, aeey ?anoaiee M. officinalis ia?aiai aiaea aeecie n oeaiiuie aaiioeie/aneeie i?eciaeaie a naeaeoeeiiiii i?ioeanna, iaiaoiaeeii aaaaoaoeaiia ?aciiiaeaiea eniieueciaaoue a eiiieaena n iaoiaeaie ii onei?aieth eo ia?aoiaea e aaia?aoeaiiio ia?eiaeo. Ia iniiaaiee ii?oioeceieiae/aneeo iniaaiiinoae ?acaeoey ceiothueo i?aaiia e iioiaaiaca, enneaaeoaiuo ni?oiia?acoeia a aa?ieeeiaoe/aneeo oneiaeyo oeaio?aeueiie e thai-ainoi/iie Oe?aeiu aeey onei?aiey ia?aoiaea e oeaaoaieth aaaaoaoeaii-?aciiiaeaiiuo ?anoaiee ia?aiai aiaea aeecie auee ?ac?aaioaiu oneiaey i?aaeiieaaathuea eniieueciaaiea oioieiaeoeoeee. I?e eco/aiee ei?iaia?aciaaiey ia caeaiuo /a?aieao M. officinalis auee ?ac?aaioaiu iooe eioaineoeeaoeee aeaiiiai i?ioeanna iin?aaenoaii eniieueciaaiey oeceieiae/ii-aeoeaiuo aauanoa a?oiiu aoeneiia. Eco/aiu iniaaiiinoe oei?aiaiey caeaiuo /a?aieia M. officinalis acyouo n aunieieoia?eiiauo e ieceieoia?eiiauo oi?i. ?ac?aaioaia nenoaia iieo/aiey eaeethniuo eoeueoo? e eiaeoeoeee ?aaaia?aoeeiiiuo i?ioeannia eenoiauo, naiyaeieueiuo e aeiieioaeaauo yenieaioia, a oaeaea eaoa?aeueiuo ii/ae. Ia iniiaaiee ?aaoeyoeee niaea?aeaiey oeoiai?iiiia a ieoaoaeueiie n?aaea iaeaeaiu iooe eiaeoeoeee ii?oiaaiaca ia eaoa?aeueiuo ii/eao aeiiieea. I?aaeeiaeaiiua iaoiaeu aeatho aiciiaeiinoue iieo/eoue enoiaeiue iaoa?eae aeey naeaeoeeiiiiai i?ioeanna, e onei?eoue iieo/aiea iiauo ni?oia. ?ac?aaioea i?aaeeaaaaiuo iaoiaeia e ?aeiiaiaeaoeee ii eo eniieueciaaieth i?iaiaeeeanue, enoiaey ec aeieiae/aneeo iniaaiiinoae aeaiiie eoeueoo?u. Iniiaiua ?acoeueoaou ?aaiou iaoee i?aeoe/aneia i?eiaiaiea i?e auaaaeaiee iiaiai ni?oa: Aeiiiee ae?eoue Eoaaineee iaeieoia?eiia?e. Eeth/aaua neiaa: aaaaoaoeaiia ?aciiiaeaiea, eeiie?iaaiea, /a?aiee, ieoaoaeueiay n?aaea, oeoiai?iiiu, ei?iaia?aciaaiea, eaeethn, eaoa?aeueiua ii/ee, yenieaiou, ?aaaia?aoeey. SUMMARY Eharchenko V.E. Biological peculiarities of Melilotus officinalis (L.)Pall at vegetative propagation. - Manuscrript This is for a candidate thesis - 03.00.05. - botany.- N.N. Grishko Central Botanical Garden of the National Academy of Sciences of Ukraine, Kyiv, 1999. The dissertstion is dedicated to elaboration of methods of a vegetative propagation of M. officinalis. The aim of it wos to promote selective works to get new sorts. The perspectives of development of regenerants and plants of different ages were stadied. The methods of speeded mate up of a large quantity of studed plants and ways of receipt of seeds from the regenerants of the first year plant were worked out. The system of receipt of callus and induction of regenerative process of leaves, seed, hipocotyle exsplants and lateral buds. Ontomorphogeny and plant regeneration from lateral buds accompanied with regulation of phytohormon content in nutrient medium was worked out. It gives a chance to get ap initial material for selection and reduce the period of a selective process. The make up of the new methods and applied recommendations was carried out on the baris of biological peculiarities of these plants. The main result of the work find a practical use for getting of the new sort Melilotus officinalis . Key words: vegetative propagation, nourishment enrollment, taking root, phytohormons, clone, callus, laterali buds, explants, regeneration.

Добавить комментарий

Ваш e-mail не будет опубликован. Обязательные поля помечены *